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[PMID]:28744579
[Au] Autor:Uda K; Abe K; Dehara Y; Mizobata K; Edashige Y; Nishimura R; Radkov AD; Moe LA
[Ad] Endereço:Laboratory of Biochemistry, Faculty of Science and Technology, Kochi University, Kochi, 780-8520, Japan. k-uda@kochi-u.ac.jp.
[Ti] Título:Triple serine loop region regulates the aspartate racemase activity of the serine/aspartate racemase family.
[So] Source:Amino Acids;49(10):1743-1754, 2017 10.
[Is] ISSN:1438-2199
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Recently, we cloned and characterized eleven serine and aspartate racemases (SerR and AspR, respectively) from animals. These SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis. Moreover, we have proposed that the AspR-specific triple serine loop region at amino acid positions 150-152 may be responsible for the large AspR activity. In the present study, to test this hypothesis, we prepared and characterized fourteen mutants in this region of animal SerRs and AspRs. The large AspR activity in Acropora and Crassostrea AspR was reduced to <0.04% of wild-type after substitution of the triple serine loop region. Conversely, introducing the triple serine loop region into Acropora, Crassostrea, and Penaeus SerR drastically increased the AspR activity. Those mutants showed similar or higher substrate affinity for aspartate than serine and showed 11-683-fold higher k and 28-351-fold higher k /K values for aspartate than serine racemization. Furthermore, we introduced serine residues in all combinations at position 150-152 in mouse SerR. These mutants revealed that a change in the enzyme function from SerR to AspR can be caused by introduction of Ser151 and Ser152, and addition of the third serine residue at position 150 further enhances the enzyme specificity for aspartate due to a decrease in the serine racemase and serine dehydratase activity. Here, we provide convincing evidence that the AspR gene has evolved from the SerR gene by acquisition of the triple serine loop region.
[Mh] Termos MeSH primário: Isomerases de Aminoácido
Antozoários
Proteínas de Artrópodes
Crassostrea
Mutação de Sentido Incorreto
Penaeidae
Racemases e Epimerases
[Mh] Termos MeSH secundário: Isomerases de Aminoácido/química
Isomerases de Aminoácido/genética
Substituição de Aminoácidos
Animais
Antozoários/enzimologia
Antozoários/genética
Proteínas de Artrópodes/química
Proteínas de Artrópodes/genética
Crassostrea/enzimologia
Crassostrea/genética
Camundongos
Penaeidae/enzimologia
Penaeidae/genética
Estrutura Secundária de Proteína
Racemases e Epimerases/química
Racemases e Epimerases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 5.1.- (Racemases and Epimerases); EC 5.1.1.- (Amino Acid Isomerases); EC 5.1.1.13 (aspartate racemase); EC 5.1.1.16 (serine racemase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1007/s00726-017-2472-8


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[PMID]:29339067
[Au] Autor:Sun Y; Zhang J; Xiang J
[Ad] Endereço:College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; College of Marine Life and Fisheries, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China.
[Ti] Título:Molecular characterization and function of ß-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda.
[So] Source:Gene;648:12-20, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chitin degradation is catalyzed by a two-component chitinolytic enzyme system, chitinase and ß-N-acetylglucosaminidase (NAGase). In this paper, the full-length cDNA sequence encoding NAGase (EcNAG) was obtained from Exopalaemon carinicauda. The deduced amino acid sequence of EcNAG open reading frame (ORF) contained one Glycohydro_20b2 domain and one Glyco_hydro_20 domain. Based on the cDNA sequence, the genomic structure of EcNAG was characterized and it was composed of six exons and five introns. EcNAG mRNA majorly expressed in the hepatopancreas and epidermis. During the molting stages, EcNAG mRNA expression was well-regulated and its expression reached the highest level at the molting stage E. In addition, EcNAG was recombinant expressed in Pichia pastoris and the partial enzymatic characterization of recombinant EcNAG was confirmed. After being challenged with Vibrio parahaemolyticus and Aeromonas hydrophila, the expression of EcNAG was up-regulated significantly at 6 h and reached the peak at 12 h. And then, the expression began to down-regulated and came to the normal level at 72 h. It is helpful to research the relationship between the molt-related hormones and chitinlytic enzymes.
[Mh] Termos MeSH primário: Acetilglucosaminidase/genética
Proteínas de Artrópodes/genética
Muda/genética
Palaemonidae/genética
[Mh] Termos MeSH secundário: Acetilglucosaminidase/classificação
Acetilglucosaminidase/metabolismo
Aeromonas hydrophila/fisiologia
Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/classificação
Proteínas de Artrópodes/metabolismo
Sequência de Bases
Epiderme/crescimento & desenvolvimento
Epiderme/metabolismo
Epiderme/microbiologia
Doenças dos Peixes/genética
Doenças dos Peixes/microbiologia
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica no Desenvolvimento
Regulação Enzimológica da Expressão Gênica
Hepatopâncreas/crescimento & desenvolvimento
Hepatopâncreas/metabolismo
Hepatopâncreas/microbiologia
Palaemonidae/crescimento & desenvolvimento
Palaemonidae/microbiologia
Filogenia
Homologia de Sequência de Aminoácidos
Vibrio parahaemolyticus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.2.1.52 (Acetylglucosaminidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:29372972
[Au] Autor:Mayor TY; Galimova YA; Sheveleva NG; Sukhanova LV; Kirilchik SV
[Ti] Título:[Molecular phylogenetic analysis of Diacyclops and Acanthocyclops (Copepoda: Cyclopoida) from Lake Baikal].
[So] Source:Genetika;53(2):233-9, 2017 Feb.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Lake Baikal is inhabited by a relatively large number of cyclopid species, many of which are endemics. Two genera, Diacyclops Kiefer, 1927 and Acanthocyclops Kiefer, 1927, are the most specious in the lake. Taxonomic discrimination of the majority of representatives of these genera is difficult owing to their high morphological similarities and poor standard description. In this study, a molecular phylogenetic analysis of Lake Baikal members of the Diacyclops/Acanthocyclops group is performed on the basis of mitochondrial cytochrome c oxidase subunit I (COI) gene. It is shown that a fragment of COI 1000 bp long is sufficient for intragenus discrimination of the cyclopids of Lake Baikal. The issues of Diacyclops/Acanthocyclops taxonomy are reflected in the obtained molecular data. Two distinct phylogenetic groups of Diacyclops genus with uncertain taxonomic status are revealed.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/genética
Copépodes/genética
DNA Mitocondrial/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Lagos
Sibéria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (DNA, Mitochondrial); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


  4 / 2217 MEDLINE  
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[PMID]:29258861
[Au] Autor:Carcamo-Noriega EN; Olamendi-Portugal T; Restano-Cassulini R; Rowe A; Uribe-Romero SJ; Becerril B; Possani LD
[Ad] Endereço:Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, UNAM, Av. Universidad 2001, Apartado Postal 510-3, Cuernavaca, Morelos 62210, Mexico. Electronic address: edsoncar@ibt.unam.mx.
[Ti] Título:Intraspecific variation of Centruroides sculpturatus scorpion venom from two regions of Arizona.
[So] Source:Arch Biochem Biophys;638:52-57, 2018 01 15.
[Is] ISSN:1096-0384
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated geographic variability in the venom of Centruroides sculpturatus scorpions from different biotopes. Venom from scorpions collected from two different regions in Arizona; Santa Rita Foothills (SR) and Yarnell (Yar) were analyzed. We found differences between venoms, mainly in the two most abundant peptides; SR (CsEv2e and CsEv1f) and Yar (CsEv2 and CsEv1c) identified as natural variants of CsEv1 and CsEv2. Sequence analyses of these peptides revealed conservative amino acid changes between variants, which may underlie biological activity against arthropods. A third peptide (CsEv6) was highly abundant in the Yar venom compared to the SR venom. CsEv6 is a 67 amino acid peptide with 8 cysteines. CsEv6 did not exhibit toxicity to the three animal models tested. However, both venoms shared similarities in peptides that are predicted to deter predators. For example, both venoms expressed CsEI (lethal to chick) in similar abundance, while CsEd and CsEM1a (toxic to mammals) displayed only moderate variation in their abundance. Electrophysiological evaluation of CsEd and CsEM1a showed that both toxins act on the human sodium-channel subtype 1.6 (hNav 1.6). Complete sequencing revealed that both toxins are structurally similar to beta-toxins isolated from different Centruroides species that also target hNav 1.6.
[Mh] Termos MeSH primário: Proteínas de Artrópodes
Variação Genética
Venenos de Escorpião
Escorpiões
[Mh] Termos MeSH secundário: Animais
Arizona
Proteínas de Artrópodes/química
Proteínas de Artrópodes/genética
Proteínas de Artrópodes/toxicidade
Células CHO
Galinhas
Cricetulus
Gryllidae
Células HEK293
Seres Humanos
Camundongos
Canal de Sódio Disparado por Voltagem NAV1.6/genética
Canal de Sódio Disparado por Voltagem NAV1.6/metabolismo
Venenos de Escorpião/química
Venenos de Escorpião/genética
Venenos de Escorpião/toxicidade
Escorpiões/química
Escorpiões/genética
Análise de Sequência de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Centruroides toxin); 0 (NAV1.6 Voltage-Gated Sodium Channel); 0 (SCN8A protein, human); 0 (Scorpion Venoms); 141322-33-0 (toxin 2, Centruroides noxius)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE


  5 / 2217 MEDLINE  
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[PMID]:29288727
[Au] Autor:Li Q; Yang H; He L; Wang Q
[Ad] Endereço:College of Ecological Engineering, Guizhou University of Engineering Science, College Road, Bijie City, Guizhou Province 551700, China.
[Ti] Título:Characterization of the Es-DDX52 involved in the spermatogonial mitosis and spermatid differentiation in Chinese mitten crab (Eriocheir sinensis).
[So] Source:Gene;646:106-119, 2018 Mar 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spermatogenesis involves a series of process including exiting from the mitotic cell cycle, entry into meiosis, completion of complex differentiation programs, and producing spermatozoa. Expression of various genes in an ordered manner, and interactions between various genes and their protein products, primarily controlled at the post-transcriptional level with DEAD-box RNA helicases playing a crucial role in germ cell development, are required for production of fertile sperm. Many members of this family have been deeply studied in spermatogenesis, such as DDX3X, DDX25 and DDX4, but few data are available on DDX52. In this study, we analyzed the expression patterns of Es-DDX52, Es-DDX6, Es-Vasa and Es-XRN1 both at mRNA and protein levels in different tissues and during gonadal development. It showed that Es-vasa, Es-DDX6 and Es-Xrn1, components of cytoplasmic foci P-bodies, have the similar transcriptional expression pattern, while Es-DDX52 has the reverse tendency. Furthermore, Es-DDX6 and Es-XRN1 proteins have the same localization in testicular tissues. Es-DDX52 mainly distributed in the cytoplasm of spermatogonia, only localized in the nucleus of early and middle spermatid and shifted to pre-acrosome vesicle (later developed into apical cap and acrosome tube) at both mRNA and protein levels. These results indicated that Es-DDX52 may participate in regulation of P-bodies and microtubules by Es-XRN1, and involved in the mitosis of spermatonia and spermatid differentiation.
[Mh] Termos MeSH primário: Braquiúros/metabolismo
RNA Helicases DEAD-box/genética
RNA Helicases DEAD-box/metabolismo
Espermátides/citologia
Espermatogônias/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/metabolismo
Diferenciação Celular
Clonagem Molecular
RNA Helicases DEAD-box/química
Regulação da Expressão Gênica
Masculino
Mitose
Modelos Moleculares
Filogenia
Espermátides/metabolismo
Espermatogênese
Espermatogônias/metabolismo
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:29228007
[Au] Autor:Thomas RG; Rivera Reyes BM; Gaston BM; Rivera Acosta NB; Bederman IR; Smith LA; Sutton MT; Wang B; Hunt JF; Bonfield TL
[Ad] Endereço:Department of Pediatrics, Division of Pulmonology, University Hospitals Cleveland Medical Center, Rainbow Babies and Children's Hospital, Cleveland, Ohio, United States of America.
[Ti] Título:Conjugation of nitrated acetaminophen to Der p1 amplifies peripheral blood monocyte response to Der p1.
[So] Source:PLoS One;12(12):e0188614, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: An association of acetaminophen use and asthma was observed in the International Study of Asthma and Allergies in Childhood study. However there are no clear mechanisms to explain an association between acetaminophen use and immunologic pathology. In acidic conditions like those in the stomach and inflamed airway, tyrosine residues are nitrated by nitrous and peroxynitrous acids. The resulting nitrotyrosine is structurally similar to 2,4-dinitrophenol and 2,4-dinitrochlorobenzene, known haptens that enhance immune responses by covalently binding proteins. Nitrated acetaminophen shares similar molecular structure. OBJECTIVE: We hypothesized the acetaminophen phenol ring undergoes nitration under acidic conditions, producing 3-nitro-acetaminophen which augments allergic responses by acting as a hapten for environmental allergens. METHODS: 3-nitro-acetaminophen was formed from acetaminophen in the presence of acidified nitrite, purified by high performance liquid chromatography, and assayed by gas-chromatography mass spectrometry. Purified 3-nitro-acetaminophen was reacted with Dermatophagoides pteronyssinus (Der p1) and analyzed by mass spectrometry to identify the modification site. Human peripheral blood mononuclear cells proliferation response was measured in response to 3-nitro-acetaminophen and to 3-nitro-acetaminophen-modified Der p1. RESULTS: Acetaminophen was modified by nitrous acid forming 3-nitro-acetaminophen over a range of different acidic conditions consistent with airway inflammation and stomach acidity. The Der p1 protein-hapten adduct creation was confirmed by liquid chromatography-mass spectrometry proteomics modifying cysteine 132. Peripheral blood mononuclear cells exposed to 3-nitro-acetaminophen-modified Der p1 had increased proliferation and cytokine production compared to acetaminophen and Der p1 alone (n = 7; p < 0.05). CONCLUSION: These data suggests 3-nitro-acetaminophen formation and reaction with Der p1 provides a mechanism by which stomach acid or infection-induced low airway pH in patients could enhance the allergic response to proteins such as Der p1.
[Mh] Termos MeSH primário: Acetaminofen/química
Antígenos de Dermatophagoides/imunologia
Proteínas de Artrópodes/imunologia
Cisteína Endopeptidases/imunologia
Monócitos/imunologia
Nitratos/química
[Mh] Termos MeSH secundário: Animais
Antígenos de Dermatophagoides/química
Proteínas de Artrópodes/química
Asma/imunologia
Cisteína Endopeptidases/química
Dermatophagoides pteronyssinus/imunologia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Dermatophagoides); 0 (Arthropod Proteins); 0 (Nitrates); 362O9ITL9D (Acetaminophen); EC 3.4.22.- (Cysteine Endopeptidases); EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188614


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[PMID]:29237574
[Au] Autor:Freire CA; Maraschi AC; Lara AF; Amado EM; Prodocimo V
[Ad] Endereço:Departamento de Fisiologia, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, PR CEP 81531-990, Brazil. Electronic address: cafreire@ufpr.br.
[Ti] Título:Late rise in hemolymph osmolality in Macrobrachium acanthurus (diadromous freshwater shrimp) exposed to brackish water: Early reduction in branchial Na /K pump activity but stable muscle HSP70 expression.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;216:69-74, 2018 Feb.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Some Macrobrachium shrimps (Caridea, Palaemonidae) are diadromous; freshwater adults are truly euryhaline, while larvae need saline water for development. Branchial Na /K -ATPase (NKA) and carbonic anhydrase (CA) are involved in NaCl absorption in freshwater. This study aimed at verifying the time course of the osmoregulatory response of adult Macrobrachium acanthurus to high salinity brackish water (20‰), from the first 30min to 5days. The goal was to detect possible transition from hyper- to hyporegulation, the putative involvement of branchial NKA and CA, or the induction of muscular HSP70 expression. Hemolymph osmotic and ionic concentrations remained relatively stable and close to control levels until ~9h of exposure, but later increased consistently (~50%). A fast reduction in NKA activity (3-6h) was observed; these shrimps seem to shut off salt absorption already in the first hours. Later on, especially after 24h, hemolymph concentrations rise but HSP70 expression is not induced, possibly because constitutive levels are already sufficient to prevent protein damage. Time-dependent response mechanisms effective in high salinity brackish water, resulting in salt loading avoidance and suggestive of hyporegulation should be further investigated in decapods that evolutionary invaded freshwater.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/biossíntese
Regulação da Expressão Gênica
Proteínas de Choque Térmico HSP70/biossíntese
Hemolinfa/metabolismo
Músculos/metabolismo
Palaemonidae/metabolismo
ATPase Trocadora de Sódio-Potássio/biossíntese
[Mh] Termos MeSH secundário: Animais
Concentração Osmolar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (HSP70 Heat-Shock Proteins); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


  8 / 2217 MEDLINE  
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[PMID]:29180238
[Au] Autor:Asaro A; Paggi RA; Del Valle JC; López Mañanes AA
[Ad] Endereço:Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, Argentina.
[Ti] Título:Glucose homeostasis in the euryhaline crab Cytograpsus angulatus: Effects of the salinity in the amylase, maltase and sucrase activities in the hepatopancreas and in the carbohydrate reserves in different tissues.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;216:39-47, 2018 Feb.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We studied the existence, biochemical characteristics and response to different environmental salinities of amylase, maltase and sucrase activity in the intertidal euryhaline crab Cyrtograpsus angulatus (Dana, 1852) along with the response to distinct salinities of glycogen and free glucose content in storage organs. Amylase, maltase and sucrase activities were kept over a broad range of pH and temperature and exhibited Michaelis-Menten kinetics. Zymography showed the existence of two amylase forms in crabs exposed to 35 (osmoconformation) and low (6-10psu; hyper-regulation) or high (40psu) (hypo-regulation) salinities. Carbohydrases activity in the hepatopancreas and glycemia were not affected in crab exposed to different environmental salinities. In 6 and 40psu, the glycogen content in anterior gills was lower than in 35psu. In 6, 10 and 40psu, glycogen concentration in hepatopancreas, muscle and posterior gills were similar to that in 35psu. Free glucose concentration in chela muscle was higher in 6 and 40psu than in 35psu. The existence and biochemical characteristics of carbohydrases activity and the adjustments in concentration of glycogen in anterior gills and free glucose in chela muscle suggests the ability to perform complete hydrolysis of glycogenic substrates and to keep glucose homeostasis in relation to acclimation to different salinity conditions.
[Mh] Termos MeSH primário: Amilases/metabolismo
Proteínas de Artrópodes/metabolismo
Braquiúros/metabolismo
Glucose/metabolismo
Hepatopâncreas/metabolismo
Salinidade
Sacarase/metabolismo
alfa-Glucosidases/metabolismo
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.2.1.- (Amylases); EC 3.2.1.20 (alpha-Glucosidases); EC 3.2.1.48 (Sucrase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


  9 / 2217 MEDLINE  
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[PMID]:28449954
[Au] Autor:Wang F; Lu X; Guo F; Gong H; Zhang H; Zhou Y; Cao J; Zhou J
[Ad] Endereço:Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
[Ti] Título:The immunomodulatory protein RH36 is relating to blood-feeding success and oviposition in hard ticks.
[So] Source:Vet Parasitol;240:49-59, 2017 Jun 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:An immunomodulatory protein designated RH36 was identified in the tick Rhipicephalus haemaphysaloides. The cDNA sequence of RH36 has 844bp and encodes a deduced protein with a predicted molecular weight of 24kDa. Bioinformatics analysis indicated that RH36 presented a degree of similarity of 34.36% with the immunomodulatory protein p36 from the tick Dermacentor andersoni. The recombinant RH36 (rRH36) expressed in Sf9 insect cells suppressed the T-lymphocyte mitogen-driven in vitro proliferation of splenocytes and the expression of several cytokines such as IL-2, IL-12, and TNF-α. Furthermore, the proliferation of splenocytes isolated from rRH36-inoculated mice was significantly lower than that in control mice, suggesting that rRH36 could directly suppress immune responses in vivo. In addition, microarray analysis of splenocytes indicated that the expression of several immunomodulatory genes was downregulated by rRH36. The silencing of the RH36 gene by RNAi led to a 37.5% decrease in the tick attachment rate 24h after placement into the rabbit ears, whereas vaccination with RH36 caused a 53.06% decrease in the tick engorgement rate. Unexpectedly, RNAi induced a significant decrease in the oviposition rate, ovary weight at day 12 after engorgement, and egg-hatching rate. The effects of RH36 on blood feeding and oviposition were further confirmed by vaccination tests using the recombinant protein. These results indicate that RH36 is a novel member of immunosuppressant proteins and affects tick blood feeding and oviposition.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/metabolismo
Dermacentor/fisiologia
Comportamento Alimentar/fisiologia
Regulação da Expressão Gênica/fisiologia
Oviposição/fisiologia
Rhipicephalus/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/genética
Sequência de Bases
Proliferação Celular
Feminino
Camundongos
Camundongos Endogâmicos BALB C
Oviposição/genética
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Coelhos
Glândulas Salivares/metabolismo
Baço/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


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[PMID]:28456485
[Au] Autor:Lanz MJ; Gonzalez MM; Efaw BJ; Harbeck RJ
[Ad] Endereço:AAADRS Clinical Research Center, Coral Gables, Florida. Electronic address: mjlanzmd@gmail.com.
[Ti] Título:Higher fractional exhaled nitric oxide and Der p 1 exposure in children with asthma living in tropical environments.
[So] Source:Ann Allergy Asthma Immunol;118(6):731-732, 2017 06.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Antígenos de Dermatophagoides/análise
Antígenos de Dermatophagoides/imunologia
Proteínas de Artrópodes/análise
Proteínas de Artrópodes/imunologia
Asma/imunologia
Cisteína Endopeptidases/análise
Cisteína Endopeptidases/imunologia
Óxido Nítrico/análise
Clima Tropical
[Mh] Termos MeSH secundário: Adolescente
Testes Respiratórios
Criança
Expiração
Feminino
Seres Humanos
Hipersensibilidade/etiologia
Hipersensibilidade/imunologia
Masculino
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Dermatophagoides); 0 (Arthropod Proteins); 31C4KY9ESH (Nitric Oxide); EC 3.4.22.- (Cysteine Endopeptidases); EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE



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