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  1 / 14063 MEDLINE  
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[PMID]:29447197
[Au] Autor:Fujita T; Kozuka-Hata H; Hori Y; Takeuchi J; Kubo T; Oyama M
[Ad] Endereço:Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
[Ti] Título:Shotgun proteomics deciphered age/division of labor-related functional specification of three honeybee (Apis mellifera L.) exocrine glands.
[So] Source:PLoS One;13(2):e0191344, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The honeybee (Apis mellifera L.) uses various chemical signals produced by the worker exocrine glands to maintain the functioning of its colony. The roles of worker postcerebral glands (PcGs), thoracic glands (TGs), and mandibular glands (MGs) and the functional changes they undergo according to the division of labor from nursing to foraging are not as well studied. To comprehensively characterize the molecular roles of these glands in workers and their changes according to the division of labor of workers, we analyzed the proteomes of PcGs, TGs, and MGs from nurse bees and foragers using shotgun proteomics technology. We identified approximately 2000 proteins from each of the nurse bee or forager glands and highlighted the features of these glands at the molecular level by semiquantitative enrichment analyses of frequently detected, gland-selective, and labor-selective proteins. First, we found the high potential to produce lipids in PcGs and MGs, suggesting their relation to pheromone production. Second, we also found the proton pumps abundant in TGs and propose some transporters possibly related to the saliva production. Finally, our data unveiled candidate enzymes involved in labor-dependent acid production in MGs.
[Mh] Termos MeSH primário: Abelhas/genética
Glândulas Exócrinas/fisiologia
Proteômica/métodos
[Mh] Termos MeSH secundário: Fatores Etários
Sequência de Aminoácidos
Animais
Abelhas/metabolismo
Comportamento Animal/fisiologia
Glândulas Exócrinas/citologia
Glândulas Exócrinas/metabolismo
Proteínas de Insetos/metabolismo
Feromônios/metabolismo
Proteoma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Pheromones); 0 (Proteome)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191344


  2 / 14063 MEDLINE  
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[PMID]:28465230
[Au] Autor:Zhu F; Xiao S; Zhang Y; Shao Y; Tang F; Chen S; Bai X
[Ad] Endereço:Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi 661101, Yunnan, China. Electronic address: 18287322700@163.com.
[Ti] Título:Molecular characterization and expression analysis of Turtle protein in silkworm that is associated with Nosema bombycis infection.
[So] Source:Infect Genet Evol;52:67-74, 2017 Aug.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this report, we describe the cloning and characterization of a member of the immunoglobulin superfamily (IgSF); i.e., Turtle. The cDNA of Turtle was cloned from the silkworm Bombyx mori using the rapid amplification of cDNA ends (RACE) technique. Three isoforms of Bombyx Turtle were obtained, including Bmtutl-464, Bmtutl-519, and Bmtutl-810. The three isoforms had identical 27-amino acid signal peptides and four extracellular immunoglobulin (Ig) domains (IgI-IgIV). Sequence similarity and phylogenic analysis indicated that Bmtutl-810 belongs to the group of insect Turtle isoforms and shares 76.2% identity with Drosophila Turtle. Quantitative real-time PCR analysis revealed that the Bombyx Turtle isoforms were expressed throughout the entire development period, the highest levels of expression of Bmtutl-464 and Bmtutl-519 were observed at the second instar larvae stage, whereas that of Bmtutl-810 peaked at the embryonic stage. The ubiquitous expression of Bmtutl-464, Bmtutl-519, and Bmtutl-810 were observed in all studied tissues, except for Bmtutl-519 in the silk gland. The expression level of Bmtutl-464 was highest in the ovary, whereas that of Bmtutl-519 and Bmtutl-810 was highest in the hemolymph. Bmtutl-519 was upregulated in BmN cells infected by Nosema bombycis, We speculated that Bombyx Turtle was not only involved in neural development in silkworm, as well as Drosophila Turtle, but was also involved in the regulation of other biological functions. For example, Bmtutl-519 might be involved in N. bombycis infection and may play an important role in the immune response of silkworms to N. bombycis infection.
[Mh] Termos MeSH primário: Bombyx/crescimento & desenvolvimento
Clonagem Molecular/métodos
Expressão Gênica
Imunoglobulinas/genética
Imunoglobulinas/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Bombyx/genética
Bombyx/metabolismo
Linhagem Celular
Regulação da Expressão Gênica no Desenvolvimento
Imunoglobulinas/química
Proteínas de Insetos/química
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
Filogenia
Domínios Proteicos
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulins); 0 (Insect Proteins); 0 (Protein Isoforms)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  3 / 14063 MEDLINE  
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[PMID]:29335414
[Au] Autor:Zhang SQ; Che LH; Li Y; Dan Liang; Pang H; Slipinski A; Zhang P
[Ad] Endereço:State Key Laboratory of Biocontrol, College of Ecology and Evolution, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510006, China.
[Ti] Título:Evolutionary history of Coleoptera revealed by extensive sampling of genes and species.
[So] Source:Nat Commun;9(1):205, 2018 01 15.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Beetles (Coleoptera) are the most diverse and species-rich group of insects, and a robust, time-calibrated phylogeny is fundamental to understanding macroevolutionary processes that underlie their diversity. Here we infer the phylogeny and divergence times of all major lineages of Coleoptera by analyzing 95 protein-coding genes in 373 beetle species, including ~67% of the currently recognized families. The subordinal relationships are strongly supported as Polyphaga (Adephaga (Archostemata, Myxophaga)). The series and superfamilies of Polyphaga are mostly monophyletic. The species-poor Nosodendridae is robustly recovered in a novel position sister to Staphyliniformia, Bostrichiformia, and Cucujiformia. Our divergence time analyses suggest that the crown group of extant beetles occurred ~297 million years ago (Mya) and that ~64% of families originated in the Cretaceous. Most of the herbivorous families experienced a significant increase in diversification rate during the Cretaceous, thus suggesting that the rise of angiosperms in the Cretaceous may have been an 'evolutionary impetus' driving the hyperdiversity of herbivorous beetles.
[Mh] Termos MeSH primário: Coleópteros/genética
Evolução Molecular
Variação Genética
Proteínas de Insetos/genética
[Mh] Termos MeSH secundário: Animais
Coleópteros/classificação
Proteínas de Insetos/classificação
Filogenia
Especificidade da Espécie
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insect Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02644-4


  4 / 14063 MEDLINE  
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[PMID]:28459996
[Au] Autor:Qiu L; Zhang B; Liu L; Wang X; Lei C; Lin Y; Zhao J; Ma W
[Ad] Endereço:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China.
[Ti] Título:The Role of p38 MAPK, JNK, and ERK in Antibacterial Responses of Chilo suppressalis (Lepidoptera: Crambidae).
[So] Source:J Econ Entomol;110(4):1460-1464, 2017 08 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mitogen-activated protein kinases (MAPKs) are conserved signal transduction pathways and broadly responsible for bacterial infection from yeast to mammals, and virus, fungi, and bacteria, specifically Bacillus thuringiensis, to insects. But little is known about the MAPK pathways in antibacterial responses in Chilo suppressalis (Walker), an important lepidopteran pest of rice. In this study, we used the bacteria of Bacillus thuringiensis, Escherichia coli, and Staphyloccocus aureus to infect C. suppressalis larvae, and the responses of MAPK pathways were analyzed. The results showed that E. coli infection induced the up-regulated expression of Csp38 and CsERK1 at 24 h postinfection (pi). Meanwhile, injection of B. thuringiensis and S. aureus resulted in strong activation of CsJNK phosphorylation at 3 h pi. These results suggest that MAPK signaling pathways play important functional roles in antibacterial responses in C. suppressalis larvae.
[Mh] Termos MeSH primário: Bacillus thuringiensis/fisiologia
Escherichia coli/fisiologia
Imunidade Inata
Proteínas de Insetos/genética
Proteínas Quinases Ativadas por Mitógeno/genética
Mariposas/imunologia
Staphylococcus aureus/fisiologia
[Mh] Termos MeSH secundário: Animais
MAP Quinases Reguladas por Sinal Extracelular/genética
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Proteínas de Insetos/metabolismo
Proteínas Quinases JNK Ativadas por Mitógeno/genética
Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo
Larva/genética
Larva/crescimento & desenvolvimento
Larva/imunologia
Larva/microbiologia
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Mariposas/genética
Mariposas/crescimento & desenvolvimento
Mariposas/microbiologia
Transdução de Sinais
Proteínas Quinases p38 Ativadas por Mitógeno/genética
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insect Proteins); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox126


  5 / 14063 MEDLINE  
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[PMID]:28449035
[Au] Autor:Zhao JJ; Zhang Y; Fan DS; Feng JN
[Ad] Endereço:Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, Northwest A&F University, Yangling 712100, Shaanxi, China.
[Ti] Título:Identification and Expression Profiling of Odorant-Binding Proteins and Chemosensory Proteins of Daktulosphaira vitifoliae (Hemiptera: Phylloxeridae).
[So] Source:J Econ Entomol;110(4):1813-1820, 2017 08 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In insects, odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are primary peripheral olfactory proteins playing critical roles in odorant detection. In this study, we present the first identification of OBPs and CSPs from the transcriptome of grape phylloxera Daktulosphaira vitifoliae Fitch, an important pest that damages both roots and leaves of grapes. The OBPs contained six conserved cysteine residues and the CSPs contained four conserved cysteine residues in this insect. Phylogenetic analysis showed that most of the olfactory proteins were closely related to OBPs and CSPs from other aphids. However, DviOBP7 and DviCSP9 were different because they were classified into different independent branches, respectively. Real-time polymerase chain reaction (RT-PCR) was used to examine the tissue expression of these transcripts. DviOBP1, DviOBP6, and DviOBP7 were uniquely or primarily expressed in antennae and not in the body. DviOBP2 was more abundantly expressed in the body than in the antennae. The expression levels of OBPs and CSPs of phylloxera varied depending upon where they were expressed in different body tissues.
[Mh] Termos MeSH primário: Hemípteros/genética
Proteínas de Insetos/genética
Receptores Odorantes/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
China
Clonagem Molecular
DNA Complementar/genética
Hemípteros/metabolismo
Proteínas de Insetos/química
Proteínas de Insetos/metabolismo
Filogenia
RNA/genética
Reação em Cadeia da Polimerase em Tempo Real
Receptores Odorantes/química
Receptores Odorantes/metabolismo
Alinhamento de Sequência
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Insect Proteins); 0 (Receptors, Odorant); 0 (odorant-binding protein); 63231-63-0 (RNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox121


  6 / 14063 MEDLINE  
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[PMID]:29381065
[Au] Autor:Lin N; Chen S; Zhang H; Li J; Fu L
[Ti] Título:Quantification of Major Royal Jelly Protein 1 in Fresh Royal Jelly by Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry.
[So] Source:J Agric Food Chem;66(5):1270-1278, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Major royal jelly protein 1 (MRJP1) is the most abundant protein in royal jelly (RJ), and the level of MRJP1 has been suggested as a promising parameter for standardization and evaluation of RJ authenticity in quality. Here, a quantitative method was developed for the quantification of MRJP1 in RJ based on a signature peptide and a stable isotope-labeled internal standard peptide FFDYDFGSDER*(R*, C , N ) by ultraperformance liquid chromatography-tandem mass spectrometry. Recoveries of the established method ranged from 85.33 to 95.80%, and both the intra- and interday precision were RSD < 4.97%. Quantification results showed that content of MRJP1 in fresh RJ was 41.96-55.01 mg/g. Abnormal levels of MRJP1 were found in three commercial RJs and implied that these samples were of low quality and might be adulterated. Results of the present work suggested that the developed method could be successfully applied to quantify MRJP1 in RJ and also could evaluate the quality of RJ.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Ácidos Graxos/química
Glicoproteínas/análise
Proteínas de Insetos/análise
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Isótopos de Carbono
Marcação por Isótopo
Isótopos de Nitrogênio
Fragmentos de Peptídeos/química
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Fatty Acids); 0 (Glycoproteins); 0 (Insect Proteins); 0 (MRJP1 protein, Apis mellifera); 0 (Nitrogen Isotopes); 0 (Peptide Fragments); L497I37F0C (royal jelly)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05698


  7 / 14063 MEDLINE  
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[PMID]:29317625
[Au] Autor:Hall MP; Woodroofe CC; Wood MG; Que I; Van't Root M; Ridwan Y; Shi C; Kirkland TA; Encell LP; Wood KV; Löwik C; Mezzanotte L
[Ad] Endereço:Promega Corporation, Madison, WI, 53711, USA.
[Ti] Título:Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging.
[So] Source:Nat Commun;9(1):132, 2018 01 09.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The sensitivity of bioluminescence imaging in animals is primarily dependent on the amount of photons emitted by the luciferase enzyme at wavelengths greater than 620 nm where tissue penetration is high. This area of work has been dominated by firefly luciferase and its substrate, D-luciferin, due to the system's peak emission (~ 600 nm), high signal to noise ratio, and generally favorable biodistribution of D-luciferin in mice. Here we report on the development of a codon optimized mutant of click beetle red luciferase that produces substantially more light output than firefly luciferase when the two enzymes are compared in transplanted cells within the skin of black fur mice or in deep brain. The mutant enzyme utilizes two new naphthyl-luciferin substrates to produce near infrared emission (730 nm and 743 nm). The stable luminescence signal and near infrared emission enable unprecedented sensitivity and accuracy for performing deep tissue multispectral tomography in mice.
[Mh] Termos MeSH primário: Benzotiazóis/metabolismo
Coleópteros/enzimologia
Proteínas de Insetos/metabolismo
Luciferases/metabolismo
[Mh] Termos MeSH secundário: Animais
Benzotiazóis/química
Células HEK293
Seres Humanos
Proteínas de Insetos/genética
Luciferases/genética
Luminescência
Medições Luminescentes/métodos
Células MCF-7
Camundongos Endogâmicos C57BL
Camundongos Nus
Microscopia de Fluorescência
Mutação
Espectroscopia de Luz Próxima ao Infravermelho
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzothiazoles); 0 (D-luciferin); 0 (Insect Proteins); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02542-9


  8 / 14063 MEDLINE  
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[PMID]:27774769
[Au] Autor:Kay S; Skowronski D; Hunt BG
[Ad] Endereço:Department of Entomology, University of Georgia, Griffin, Georgia, USA.
[Ti] Título:Developmental DNA methyltransferase expression in the fire ant Solenopsis invicta.
[So] Source:Insect Sci;25(1):57-65, 2018 Feb.
[Is] ISSN:1744-7917
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:DNA methylation is accomplished in animals by 2 classes of enzymes known as DNA methyltransferases, DNMT3 and DNMT1, which perform de novo methylation and maintenance methylation, respectively. Several studies of hymenopteran eusocial insects suggest that DNA methylation is capable of influencing developmental plasticity. However, fundamental questions remain about the patterning of DNA methylation during the course of insect development. In this study, we performed quantitative real-time PCR (qPCR) on transcripts from the single-copy orthologs of DNMT1 and DNMT3 in the red imported fire ant, Solenopsis invicta. In particular, we assessed the expression of S. invicta Dnmt1 and Dnmt3 mRNA during 7 stages of worker development, among behaviorally distinct adults, and among male and female gonads. Dnmt3 was most highly expressed during embryonic development, whereas Dnmt1 was similarly expressed throughout the course of development. Moreover, Dnmt1 and Dnmt3 were highly expressed in testes and ovaries. Neither Dnmt was significantly differentially expressed among heads of behaviorally distinct adult castes. Our results support the hypothesis that extensive patterning of DNA methylation occurs during gametogenesis and embryogenesis in the insect order Hymenoptera.
[Mh] Termos MeSH primário: Formigas/enzimologia
Formigas/crescimento & desenvolvimento
DNA (Citosina-5-)-Metiltransferases/metabolismo
Metilação de DNA
[Mh] Termos MeSH secundário: Animais
Feminino
Proteínas de Insetos/metabolismo
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1111/1744-7917.12413


  9 / 14063 MEDLINE  
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[PMID]:29355529
[Au] Autor:Wang X; Wang JG; Geng YY; Wang JJ; Zhang XM; Yang SS; Jiang W; Liu WQ
[Ad] Endereço:State Key Laboratories of Agrobiotechnology, College of Biological Sciences, China Agricultural University, PR China.
[Ti] Título:An enhanced anti-tumor effect of apoptin-cecropin B on human hepatoma cells by using bacterial magnetic particle gene delivery system.
[So] Source:Biochem Biophys Res Commun;496(2):719-725, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gene therapy of cancer, due to the limit of its efficiency and safety, has not been widely used in clinical. Recently, bacterial magnetic particles (BMPs), which are membrane-bound nanocrystals found in magnetotactic bacteria, have been exploited as a new gene delivery system. However, its application on gene therapy remains to be explored. In our previous study, we found that a combination of cecropin B (ABPs) and apoptin (VP3) could serve as an effective gene therapeutic agent. Thus, in this study, we used BMPs to deliver the co-expression plasmid of these two gene, namely pVAX1-VA, and evaluated its therapeutic effect on human hepatocellular carcinoma (HepG2). Our results showed that BMPs significantly improved the efficiency of gene transfection (almost 3-fold than Lipofectamine 2000 at 48 h, P < .001), which led to stronger apoptosis (in a peak almost 2-fold than Lipofectamine 2000-pVAX1-VA, P < .01) and growth inhibition of HepG2 cells. More importantly, compared with Lipofectamine 2000-pVAX1-VA group, BMP-pVAX1-VA strikingly inhibited tumor growth (0.60 ±â€¯0.09 g vs. 0.88 ±â€¯0.11 g, P < .05) in nude mouse tumor models and increased the tumor-infiltrating lymphocytes considerably without apparent cytotoxicity. These findings suggest that BMPs could be an attractive gene delivery system for gene therapy and provide a potential available treatment for human hepatocellular carcinoma and maybe some other kinds of tumors.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/genética
Carcinoma Hepatocelular/terapia
Técnicas de Transferência de Genes
Vetores Genéticos/administração & dosagem
Proteínas de Insetos/genética
Neoplasias Hepáticas/terapia
Magnetossomos/química
Magnetospirillum/química
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/metabolismo
Carcinoma Hepatocelular/genética
Carcinoma Hepatocelular/patologia
Portadores de Fármacos/química
Feminino
Terapia Genética/métodos
Vetores Genéticos/genética
Vetores Genéticos/uso terapêutico
Células Hep G2
Seres Humanos
Neoplasias Hepáticas/genética
Neoplasias Hepáticas/patologia
Camundongos Endogâmicos BALB C
Camundongos Nus
Transfecção/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Capsid Proteins); 0 (Drug Carriers); 0 (Insect Proteins); 0 (VP3 protein, Chicken anemia virus); 80451-05-4 (cecropin B protein, Insecta)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE


  10 / 14063 MEDLINE  
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[PMID]:29265467
[Au] Autor:Guan J; Zhang J; Yuan S; Yang B; Clark KD; Ling E; Huang W
[Ad] Endereço:Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
[Ti] Título:Analysis of the functions of the signal peptidase complex in the midgut of Tribolium castaneum.
[So] Source:Arch Insect Biochem Physiol;97(3), 2018 Mar.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Signal peptidase complexes (SPCs) are conserved from bacteria to human beings, and are typically composed of four to five subunits. There are four genes encoding SPC proteins in the red flour beetle, Tribolium castaneum. To understand their importance to insect development, double-stranded RNA for each SPC gene was injected into red flour beetles at the early larval and adult stages. Knockdown of all four signal peptidase genes was lethal to larvae. Moreover, larvae had difficulty with old cuticle ecdysis. Knockdown of TcSPC12 alone did not affect pupal or adult development. When TcSPC12, TcSPC18, and TcSPC25 were knocked down in larvae, the melanization of hemocytes and midguts was observed. When knocked down in larvae and adults, TcSPC18 induced severe cell apoptosis in midguts, and the adult midgut lost the ability to maintain crypts after knockdown of TcSPC18, indicating its importance to midgut cell proliferation and differentiation. Knockdown of TcSPC22 or TcSPC25 also resulted in many apoptotic cells in the midguts. However, TcSPC12 appeared to be unimportant for midgut development. We conclude that TcSPC18 is essential for maintaining the adult midgut crypts.
[Mh] Termos MeSH primário: Proteínas de Membrana/metabolismo
Serina Endopeptidases/metabolismo
Tribolium/enzimologia
[Mh] Termos MeSH secundário: Animais
Feminino
Trato Gastrointestinal/enzimologia
Hemócitos/metabolismo
Proteínas de Insetos/metabolismo
Melaninas/metabolismo
Interferência de RNA
Tribolium/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Melanins); 0 (Membrane Proteins); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.89 (type I signal peptidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21441



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