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[PMID]: | 28806780 |
[Au] Autor: | Santiago AE; Yan MB; Hazen TH; Sauder B; Meza-Segura M; Rasko DA; Kendall MM; Ruiz-Perez F; Nataro JP |
[Ad] Endereço: | Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, Virginia, United States of America. |
[Ti] Título: | The AraC Negative Regulator family modulates the activity of histone-like proteins in pathogenic bacteria. |
[So] Source: | PLoS Pathog;13(8):e1006545, 2017 Aug. | [Is] ISSN: | 1553-7374 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | The AraC Negative Regulators (ANR) comprise a large family of virulence regulators distributed among diverse clinically important Gram-negative pathogens, including Vibrio spp., Salmonella spp., Shigella spp., Yersinia spp., Citrobacter spp., and pathogenic E. coli strains. We have previously reported broad effects of the ANR members on regulators of the AraC/XylS family. Here, we interrogate possible broader effects of the ANR members on the bacterial transcriptome. Our studies focused on Aar (AggR-activated regulator), an ANR family archetype in enteroaggregative E. coli (EAEC) isolate 042. Transcriptome analysis of EAEC strain 042, 042aar and 042aar(pAar) identified more than 200 genes that were differentially expressed (+/- 1.5 fold, p<0.05). Most of those genes are located on the bacterial chromosome (195 genes, 92.85%), and are associated with regulation, transport, metabolism, and pathogenesis, based on the predicted annotation; a considerable number of Aar-regulated genes encoded for hypothetical proteins (46 genes, 21.9%) and regulatory proteins (25, 11.9%). Notably, the transcriptional expression of three histone-like regulators, H-NS (orf1292), H-NS homolog (orf2834) and StpA, was down-regulated in the absence of aar and may explain some of the effects of Aar on gene expression. By employing a bacterial two-hybrid system, LacZ reporter assays, pull-down and electrophoretic mobility shift assay (EMSA) analysis, we demonstrated that Aar binds directly to H-NS and modulates H-NS-induced gene silencing. Importantly, Aar was highly expressed in the mouse intestinal tract and was found to be necessary for maximal H-NS expression. In conclusion, this work further extends our knowledge of genes under the control of Aar and its biological relevance in vivo. |
[Mh] Termos MeSH primário: |
Fator de Transcrição AraC/metabolismo Escherichia coli Enteropatogênica/metabolismo Infecções por Escherichia coli/metabolismo Regulação Bacteriana da Expressão Gênica/fisiologia Virulência/fisiologia
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[Mh] Termos MeSH secundário: |
Animais Ensaio de Desvio de Mobilidade Eletroforética Escherichia coli Enteropatogênica/patogenicidade Proteínas de Escherichia coli/metabolismo Histonas/metabolismo Camundongos Camundongos Endogâmicos BALB C Reação em Cadeia da Polimerase
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (AraC Transcription Factor); 0 (Escherichia coli Proteins); 0 (Histones) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171002 |
[Lr] Data última revisão:
| 171002 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170815 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.ppat.1006545 |
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