Base de dados : MEDLINE
Pesquisa : D12.776.097.151.743 [Categoria DeCS]
Referências encontradas : 128 [refinar]
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[PMID]:28213030
[Au] Autor:Bruschi C; Komora N; Castro SM; Saraiva J; Ferreira VB; Teixeira P
[Ad] Endereço:CBQF - Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Centro Regional do Porto da Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital Apartado 2511, 4202-401 Porto, Portugal.
[Ti] Título:High hydrostatic pressure effects on Listeria monocytogenes and L. innocua: Evidence for variability in inactivation behaviour and in resistance to pediocin bacHA-6111-2.
[So] Source:Food Microbiol;64:226-231, 2017 Jun.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effect of high hydrostatic pressure (HHP) on the survival of 14 strains of Listeria monocytogenes from food or clinical origins, selected to represent different pheno and genotypes, was evaluated. Stationary phase cells were submitted to 300, 400 and 500 MPa at 10 °C, for 5 min. A high variability in the resistance of L. monocytogenes to pressure was observed, and particularly two strains isolated from food were significantly more baroresistant than the rest. Strains of L. monocytogenes resistant to one or more antibiotics exhibited significantly higher levels of survival after the high pressure treatment at 400 MPa. No correlation was found between strains' origin or thermal tolerance and resistance to HHP. The suitability of two strains of L. innocua as surrogates of L. monocytogenes, was also investigated. These exhibited significantly higher sensitivities to HHP than observed for some L. monocytogenes. The antimicrobial effect of the antilisterial bacteriocin (bacHA-6111-2) increased after L. monocytogenes cells had been exposed to pressure. The data obtained underlines the importance of strain selection for studies aiming to evaluate HHP efficacy to ensure safety of HHP-treated foods.
[Mh] Termos MeSH primário: Pressão Hidrostática
Listeria monocytogenes/efeitos dos fármacos
Listeria monocytogenes/fisiologia
Viabilidade Microbiana
Pediocinas/farmacologia
[Mh] Termos MeSH secundário: Contagem de Colônia Microbiana
Contaminação de Alimentos/prevenção & controle
Microbiologia de Alimentos
Inocuidade dos Alimentos
Listeria monocytogenes/crescimento & desenvolvimento
Listeriose/microbiologia
Termotolerância
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pediocins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


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[PMID]:28027492
[Au] Autor:Wang X; Mao J; Chen Y; Song D; Gao Z; Zhang X; Bai Y; Saris PE; Feng H; Xu H; Qiao M
[Ad] Endereço:The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
[Ti] Título:Design of antibacterial biointerfaces by surface modification of poly (ε-caprolactone) with fusion protein containing hydrophobin and PA-1.
[So] Source:Colloids Surf B Biointerfaces;151:255-263, 2017 Mar 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Class IIa bacteriocin pediocin PA-1 has broad-spectrum activity and is a well-characterized candidate food biopreservative. Here, a simple approach is designed to extend the application of pediocin PA-1 in improving the antibacterial activity of electrospun poly(caprolactone) (PCL) grafts through combining PA-1 with HGFI, which is a self-assembled protein with characteristics allowing the modulation of surface properties of other materials originated from Grifola frondosa. Saccharomyces cerevisiae was used as the host for expression of fusion protein PA-1-linker-HGFI (pH) and his-tag purification was used to purify recombinant protein pH. An antibacterial activity assay showed the fusion protein pH retained the biological property of native PA-1. Water contact angle, X-ray photoelectron spectroscopy, immunofluorescence assay and atomic force microscopy indicated the surface properties of HGFI were greatly preserved by the fusion protein pH. Finally, antibacterial activity of pH-modified PCL substrate measurements implied the fusion protein significantly improved the bacterial-resistance of the PCL film through dressing the PCL fibers with the recombinant pH protein. This work presents a new perspective on the application of hydrophobin and pediocin PA-1 in antibacterial medical devices.
[Mh] Termos MeSH primário: Antibacterianos/química
Caproatos/química
Lactonas/química
Pediocinas/química
[Mh] Termos MeSH secundário: Animais
Anticorpos/química
Bacteriocinas/química
Biofilmes
Equipamentos e Provisões
Conservantes de Alimentos
Grifola/química
Concentração de Íons de Hidrogênio
Camundongos
Microscopia de Força Atômica
Microscopia Eletrônica de Varredura
Microscopia de Fluorescência
Espectroscopia Fotoeletrônica
Proteínas Recombinantes de Fusão/química
Saccharomyces cerevisiae
Propriedades de Superfície
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antibodies); 0 (Bacteriocins); 0 (Caproates); 0 (Food Preservatives); 0 (Lactones); 0 (Pediocins); 0 (Recombinant Fusion Proteins); 111745-56-3 (pediocin PA-1); 56RE988L1R (caprolactone)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161228
[St] Status:MEDLINE


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[PMID]:27522409
[Au] Autor:Amado IR; Fuciños C; Fajardo P; Pastrana L
[Ad] Endereço:Departamento de Química Analítica y Alimentaria, Facultad de Ciencias de Ourense, Universidad de Vigo, As Lagoas s/n 32004, Spain. Electronic address: sabelara@uvigo.es.
[Ti] Título:Pediocin SA-1: A selective bacteriocin for controlling Listeria monocytogenes in maize silages.
[So] Source:J Dairy Sci;99(10):8070-8080, 2016 Oct.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we assessed the potential as silage additive of a bacteriocin produced by Pediococcus acidilactici Northern Regional Research Laboratory (NRRL) B-5627 (pediocin SA-1). Maize was inoculated either with a bacterial starter alone (I) or in combination with the bacteriocin (IP), and untreated silage served as control. We monitored the products of fermentation (ethanol, and lactic and acetic acids), the microbial population, and the presence of the indicator strain Listeria monocytogenes Colección Española de Cultivos Tipo (CECT) 4032 (1×10(5) cfu/g) after 1, 2, 5, 8, 16, and 30d of ensiling. Our results indicated antilisterial activity of the bacteriocin, anticipating the disappearance of L. monocytogenes in IP compared with I and control silages. The PCR-denaturing gradient gel electrophoresis analysis revealed the addition of the bacteriocin did not affect the bacterial communities of the spontaneous fermentation, and the inoculant-containing bacteria (Lactobacillus plantarum, Lactobacillus buchneri, and Enterococcus faecium) were found in addition to the bacterial communities of untreated maize silages in I and IP silages. Both treatments increased the concentration of antimicrobial compounds (acetic acid, ethanol, and 1,2-propanodiol) and led to lower residual sugar contents compared with the control, which would provide enhanced aerobic stability. The fact that the identified species L. plantarum, L. buchneri, and E. faecium produce some of these inhibitory compounds, together with their persistence throughout the 30d of fermentation, suggest these bacteria could actively participate in the ensiling process. According to these results, pediocin SA-1 could be used as an additive to control the presence of L. monocytogenes in maize silages selectively, while improving their fermentative quality and eventually their aerobic stability.
[Mh] Termos MeSH primário: Listeria monocytogenes/efeitos dos fármacos
Pediocinas/farmacologia
Silagem/microbiologia
Zea mays/microbiologia
[Mh] Termos MeSH secundário: Ácido Acético/análise
Antibacterianos/farmacologia
DNA Bacteriano/isolamento & purificação
Enterococcus faecium/metabolismo
Etanol/análise
Fermentação
Concentração de Íons de Hidrogênio
Lactobacillus delbrueckii/metabolismo
Lactobacillus plantarum/metabolismo
Pediococcus acidilactici/metabolismo
Silagem/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Pediocins); 3K9958V90M (Ethanol); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160815
[St] Status:MEDLINE


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[PMID]:27378130
[Au] Autor:Meena S; Mehla J; Kumar R; Sood SK
[Ad] Endereço:Division of Animal Biochemistry, National Dairy Research Institute, Karnal, Haryana, 132001, India. sunitameena1188@gmail.com.
[Ti] Título:Common Mechanism of Cross-Resistance Development in Pathogenic Bacteria Bacillus cereus Against Alamethicin and Pediocin Involves Alteration in Lipid Composition.
[So] Source:Curr Microbiol;73(4):534-41, 2016 Oct.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To understand the mechanism of development of cross-resistance in food pathogen Bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. Pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followed by cation exchange chromatography with 14.01-fold purity and 14.4 % recovery. B. cereus strains alamethicin-resistant strains (IC50 3.23 µg/ml) were selected from sensitive population with IC50 2.37 µg/ml. The development of resistance in B. cereus against alamethicin was associated with decrease in alamethicin-membrane interaction observed by in vitro assay. Resistant strain of B. cereus was found to harbour one additional general lipid as compared to sensitive strain, one amino group lacking phospholipid and one amino group containing phospholipid (ACP). In addition, ACP content was increased in resistant mutant (29.7 %) as compared to sensitive strain (14.56 %). The alamethicin-resistant mutant B. cereus also showed increased IC50 (58.8 AU/ml) for pediocin as compared to sensitive strain (IC50 47.8 AU/ml). Cross-resistance to pediocin and alamethicin in resistant mutant of B. cereus suggested a common mechanism of resistance. Therefore, this understanding could result in the development of peptide which will be effective against the resistant strains that share same mechanism of resistance.
[Mh] Termos MeSH primário: Alameticina/farmacologia
Antibacterianos/farmacologia
Bacillus cereus/efeitos dos fármacos
Bacillus cereus/metabolismo
Farmacorresistência Bacteriana
Pediocinas/farmacologia
Fosfolipídeos/metabolismo
[Mh] Termos MeSH secundário: Alameticina/isolamento & purificação
Alameticina/metabolismo
Antibacterianos/isolamento & purificação
Antibacterianos/metabolismo
Bacillus cereus/química
Bacillus cereus/genética
Pediocinas/isolamento & purificação
Pediocinas/metabolismo
Pediococcus/química
Pediococcus/metabolismo
Fosfolipídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Pediocins); 0 (Phospholipids); 27061-78-5 (Alamethicin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160706
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-016-1090-0


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[PMID]:26687333
[Au] Autor:Ma X; Wang G; Li D; Hao Y
[Ad] Endereço:Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qing Hua East Road, Hai Dian District, Beijing, 100083, China.
[Ti] Título:Microcin V Production in Lactobacillus plantarum LB-B1 Using Heterologous Leader Peptide from Pediocin PA-1.
[So] Source:Curr Microbiol;72(3):357-62, 2016 Mar.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lactobacillus strains producing bacteriocins have attracted highly attention as probiotic cultures in animal nutrition since the use of antibiotics was forbidden in the livestock industry. Lactobacillus plantarum LB-B1 isolated from the fermented dairy product can produce pediocin PA-1, which has a strong inhibition of Listeria but hardly any influence on Gram-negative spoilage agents. In this work, L. plantarum LB-B1 was selected as the host to express microcin V using the leader peptide of pediocin PA-1. Well-diffusion assay combined with Tricine-SDS-polyacrylamide gel showed that microcin V could be successfully expressed and secreted in L. plantarum LB-B1. Meanwhile, the production of microcin V did not affect the secretion of pediocin PA-1. It is worthwhile noted that the supernatant from L. plantarum 8148-ColV had a more effective inhibition of Listeria than that from the control strain L. plantarum 8148. Furthermore, this supernatant also unexpectedly produced antibacterial activity against Staphylococcus aureus. Taken altogether, these results suggested that pediocin PA-1 and microcin V in the supernatant could generate synergistic effect, which not only enhanced the antibacterial ability but also expanded the antibacterial spectrum. Therefore, the recombinant strain has a great potential application as a probiotic to reduce the level of enteric pathogens in livestock industry.
[Mh] Termos MeSH primário: Bacteriocinas/genética
Bacteriocinas/metabolismo
Lactobacillus plantarum/metabolismo
Sinais Direcionadores de Proteínas
[Mh] Termos MeSH secundário: Lactobacillus plantarum/genética
Listeria/efeitos dos fármacos
Listeria/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
Pediocinas
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacteriocins); 0 (Pediocins); 0 (Protein Sorting Signals); 0 (Recombinant Proteins); 111745-56-3 (pediocin PA-1); 1403-96-9 (microcin)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151222
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-015-0927-2


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[PMID]:26162534
[Au] Autor:Fernandez B; Savard P; Fliss I
[Ad] Endereço:STELA Dairy Research Center, Nutrition and Functional Foods Institute, Université Laval, G1K 7P4, Québec, QC, Canada.
[Ti] Título:Survival and Metabolic Activity of Pediocin Producer Pediococcus acidilactici UL5: Its Impact on Intestinal Microbiota and Listeria monocytogenes in a Model of the Human Terminal Ileum.
[So] Source:Microb Ecol;72(4):931-942, 2016 11.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pediococcus acidilactici UL5 is a promising probiotic candidate due to its high survival rate under gastric and duodenal conditions and to its ability to produce the antilisterial bacteriocin pediocin PA-1. Its survival, metabolic activity, and impact on Listeria monocytogenes in a continuous stirred tank reactor containing immobilized human intestinal microbiota were studied over a period of 32 days of feeding a nutrient medium simulating ileal chyme. The impact of P. acidilactici UL5 on different bacterial groups of intestinal origin as well as its survival and its impact on L. monocytogenes were quantified using quantitative polymerase chain reaction coupling with propidium monoazide (PMA-qPCR), which was shown to detect and quantify viable bacteria only. P. acidilactici UL5 and its non-pediocin-producing mutant had no effect on the microbiota, but the producing strain induced an increase in the production of acetic and propionic acids. P. acidilactici survived but appeared to be a poor competitor with intestinal microbiota, dropping by 1.3 and 2.8 log after 8 h of fermentation to 10 colony-forming units (cfu) mL . A 1.64 log but non-significant reduction of Listeria was observed when P. acidilactici UL5 was added at 10 cfu mL . P. acidilactici UL5 isolated from the reactor after 3 days was still able to produce the active bacteriocin. These data demonstrate that P. acidilactici UL5 is capable of surviving transit through the ileum without losing its ability to produce pediocin PA-1 but seems to not be enough competitive with the great diversity of organisms found in the ileum.
[Mh] Termos MeSH primário: Microbioma Gastrointestinal/fisiologia
Íleo/microbiologia
Listeria monocytogenes/crescimento & desenvolvimento
Pediocinas/metabolismo
Pediococcus acidilactici/metabolismo
Probióticos/metabolismo
[Mh] Termos MeSH secundário: Azidas/farmacologia
Fermentação
Seres Humanos
Propídio/análogos & derivados
Propídio/farmacologia
RNA Ribossômico 16S/genética
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Azides); 0 (Pediocins); 0 (RNA, Ribosomal, 16S); 0 (propidium monoazide); 111745-56-3 (pediocin PA-1); 36015-30-2 (Propidium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150712
[St] Status:MEDLINE


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[PMID]:26147827
[Au] Autor:Back A; Borges F; Mangavel C; Paris C; Rondags E; Kapel R; Aymes A; Rogniaux H; Pavlovic M; van Heel AJ; Kuipers OP; Revol-Junelles AM; Cailliez-Grimal C
[Ad] Endereço:Laboratoire d'Ingénierie des Biomolécules (LIBio), ENSAIA, Université de Lorraine, 2 Avenue de la Forêt de Haye, VandÅ“uvre-lès-Nancy, 54518, France.
[Ti] Título:Recombinant pediocin in Lactococcus lactis: increased production by propeptide fusion and improved potency by co-production with PedC.
[So] Source:Microb Biotechnol;9(4):466-77, 2016 07.
[Is] ISSN:1751-7915
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe the impact of two propeptides and PedC on the production yield and the potency of recombinant pediocins produced in Lactococcus lactis. On the one hand, the sequences encoding the propeptides SD or LEISSTCDA were inserted between the sequence encoding the signal peptide of Usp45 and the structural gene of the mature pediocin PA-1. On the other hand, the putative thiol-disulfide oxidoreductase PedC was coexpressed with pediocin. The concentration of recombinant pediocins produced in supernatants was determined by enzyme-linked immunosorbent assay. The potency of recombinant pediocins was investigated by measuring the minimal inhibitory concentration by agar well diffusion assay. The results show that propeptides SD or LEISSTCDA lead to an improved secretion of recombinant pediocins with apparently no effect on the antibacterial potency and that PedC increases the potency of recombinant pediocin. To our knowledge, this study reveals for the first time that pediocin tolerates fusions at the N-terminal end. Furthermore, it reveals that only expressing the pediocin structural gene in a heterologous host is not sufficient to get an optimal potency and requires the accessory protein PedC. In addition, it can be speculated that PedC catalyses the correct formation of disulfide bonds in pediocin.
[Mh] Termos MeSH primário: Lactococcus lactis/genética
Lactococcus lactis/metabolismo
Engenharia Metabólica
Pediocinas/genética
Pediocinas/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Mh] Termos MeSH secundário: Ensaio de Imunoadsorção Enzimática
Redes e Vias Metabólicas/genética
Testes de Sensibilidade Microbiana
Pediocinas/análise
Proteínas Recombinantes/análise
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pediocins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171127
[Lr] Data última revisão:
171127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150707
[St] Status:MEDLINE
[do] DOI:10.1111/1751-7915.12285


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[PMID]:26596086
[Au] Autor:Klyachko EV; Morozkina EV; Zaitchik BTs; Benevolensky SV
[Ti] Título:[Distiller Yeasts Producing Antibacterial Peptides].
[So] Source:Prikl Biokhim Mikrobiol;51(5):495-501, 2015 Sep-Oct.
[Is] ISSN:0555-1099
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:A new method of controlling lactic acid bacteria contamination was developed with the use of recombinant Saccharomyces cerevisiae strains producing antibacterial peptides. Genes encoding the antibacterial peptides pediocin and plantaricin with codons preferable for S. cerevisiae were synthesized, and a system was constructed for their secretory expression. Recombinant S. cerevisiae strains producing antibacterial peptides effectively inhibit the growth of Lactobacillus sakei, Pediacoccus pentasaceus, Pediacoccus acidilactici, etc. The application of distiller yeasts producing antibacterial peptides enhances the ethanol yield in cases of bacterial contamination. Recombinant yeasts producing the antibacterial peptides pediocin and plantaricin can successfully substitute the available industrial yeast strains upon ethanol production.
[Mh] Termos MeSH primário: Etanol/síntese química
Biossíntese Peptídica
Peptídeos/genética
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Bacteriocinas/biossíntese
Bacteriocinas/genética
Fermentação
Lactobacillus/efeitos dos fármacos
Pediocinas
Peptídeos/farmacologia
Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacteriocins); 0 (Pediocins); 0 (Peptides); 0 (pediocin 5); 131463-18-8 (plantaricin A); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151125
[St] Status:MEDLINE


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[PMID]:26299998
[Au] Autor:Sun L; Song H; Zheng W
[Ti] Título:Improvement of Antimicrobial Activity of Pediocin PA-1 by Site-directed Mutagenesis in C-terminal Domain.
[So] Source:Protein Pept Lett;22(11):1007-12, 2015.
[Is] ISSN:1875-5305
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Pediocin PA-1 is a well-known Class IIa bacteriocin which shows strong inhibitory effect against Listeria monocytogenes. In this work, in order to improve the antimicrobial activity, eight single- site mutants and six combination mutants on nine interesting sites in the C-terminal region of pediocin PA-1 were constructed and expressed in Escherichia coli heterologously. Ten mutants demonstrated enhancement activity when performed the agar diffusion test to the indicator strain L. monocytogenes. The substitution of glycine in position 29 to alanine showed the most distinct increase of antimicrobial activity which clarified that 29G acted as a significant role as to guide the pediocin PA-1 molecule to dip into receptor membrane. Combination mutants of sites 29G to 32A illustrated that a hydrophobic tip of the hairpin-like structure and a smaller bundle of the α-helix domain facilitate the penetrating of pediocin PA-1 into a hydrophobic domain of the membrane-embedded subunits of the mannose-phosphotransferase system (MPTs).
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Bacteriocinas/genética
Bacteriocinas/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Anti-Infecciosos/farmacologia
Bacteriocinas/química
Listeria monocytogenes/efeitos dos fármacos
Dados de Sequência Molecular
Mutagênese Sítio-Dirigida
Pediocinas
Estrutura Terciária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Bacteriocins); 0 (Pediocins); 111745-56-3 (pediocin PA-1)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150825
[St] Status:MEDLINE


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[PMID]:25961806
[Au] Autor:Oppegård C; Fimland G; Anonsen JH; Nissen-Meyer J
[Ad] Endereço:†Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, P.O. Box 1066, Blindern, 0316 Oslo, Norway.
[Ti] Título:The Pediocin PA-1 Accessory Protein Ensures Correct Disulfide Bond Formation in the Antimicrobial Peptide Pediocin PA-1.
[So] Source:Biochemistry;54(19):2967-74, 2015 May 19.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peptides, in contrast to proteins, are generally not large enough to form stable and well-defined three-dimensional structures. However, peptides are still able to form correct disulfide bonds. Using pediocin-like bacteriocins, we have examined how this may be achieved. Some pediocin-like bacteriocins, such as pediocin PA-1 and sakacin P[N24C+44C], have four cysteines. There are three possible ways by which the four cysteines may combine to form two disulfide bonds, and the three variants are expected to be produced in approximately equal amounts if their formation is random. Pediocin PA-1 and sakacin P[N24C+44C] with correct disulfide bonds were the main products when they were secreted by the pediocin PA-1 ABC transporter and accessory protein, but when they were secreted by the corresponding secretion machinery for sakacin A, a pediocin-like bacteriocin with one disulfide bond (two cysteines), peptides with all three possible disulfide bonds were produced in approximately equal amounts. All five cysteines in the pediocin PA-1 ABC transporter and the two cysteines (that form a CxxC motif) in the accessory protein were individually replaced with serines to examine their involvement in disulfide bond formation in pediocin PA-1. The Cys86Ser mutation in the accessory protein caused a 2-fold decrease in the amount of pediocin PA-1 with correct disulfide bonds, while the Cys83Ser mutation nearly abolished the production of pediocin PA-1 and resulted in the production of all three disufide bond variants in equal amounts. The Cys19Ser mutation in the ABC transporter completely abolished secretion of pediocin PA-1, suggesting that Cys19 is in the proteolytic active site and involved in cleaving the prebacteriocin. Replacing the other four cysteines in the ABC transporter with serines caused a slight reduction in the overall amount of secreted pediocin PA-1, but the relative amount with the correct disulfide bonds remained large. These results indicate that the pediocin PA-1 accessory protein has a chaperone-like activity in that it ensures the formation of the correct disulfide bond in pediocin PA-1.
[Mh] Termos MeSH primário: Bacteriocinas/química
Peptídeos/química
[Mh] Termos MeSH secundário: Antibacterianos/química
Bacteriocinas/genética
Dissulfetos/química
Mutação
Pediocinas
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacteriocins); 0 (Disulfides); 0 (Pediocins); 0 (Peptides); 111745-56-3 (pediocin PA-1); 146240-19-9 (sakacin P protein, Lactobacillus)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150512
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.5b00164



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