Base de dados : MEDLINE
Pesquisa : D12.776.097.151.785 [Categoria DeCS]
Referências encontradas : 330 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 33 ir para página                         

  1 / 330 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28973027
[Au] Autor:Paskevicius S; Starkevic U; Misiunas A; Vitkauskiene A; Gleba Y; Razanskiene A
[Ad] Endereço:Nomads UAB, Gelezinio vilko 29A, Vilnius, Lithuania.
[Ti] Título:Plant-expressed pyocins for control of Pseudomonas aeruginosa.
[So] Source:PLoS One;12(10):e0185782, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The emergence, persistence and spread of antibiotic-resistant human pathogenic bacteria heralds a growing global health crisis. Drug-resistant strains of gram-negative bacteria, such as Pseudomonas aeruginosa, are especially dangerous and the medical and economic burden they impose underscore the critical need for finding new antimicrobials. Recent studies have demonstrated that plant-expressed bacteriocins of the colicins family can be efficient antibacterials against all major enteropathogenic strains of E. coli. We extended our studies of colicin-like bacteriocins to pyocins, which are produced by strains of P. aeruginosa for ecological advantage against other strains of the same species. Using a plant-based transient expression system, we expressed six different pyocins, namely S5, PaeM, L1, L2, L3 and one new pyocin, PaeM4, and purified them to homogeneity. Among these pyocins, PaeM4 demonstrated the broadest spectrum of activity by controlling 53 of 100 tested clinical isolates of P. aeruginosa. The activity of plant-made pyocins was confirmed in the agar drop, liquid culture susceptibility and biofilm assays, and in the Galleria mellonella animal infection model.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bacteriocinas/farmacologia
Extratos Vegetais/farmacologia
Pseudomonas aeruginosa/efeitos dos fármacos
Piocinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Biofilmes/efeitos dos fármacos
Mariposas/microbiologia
Pseudomonas aeruginosa/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacteriocins); 0 (Plant Extracts); 0 (Pyocins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185782


  2 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28052848
[Au] Autor:Chen F; Chen G; Liu Y; Jin Y; Cheng Z; Liu Y; Yang L; Jin S; Wu W
[Ad] Endereço:State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
[Ti] Título:Pseudomonas aeruginosa Oligoribonuclease Contributes to Tolerance to Ciprofloxacin by Regulating Pyocin Biosynthesis.
[So] Source:Antimicrob Agents Chemother;61(3), 2017 Mar.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial oligoribonuclease (Orn) is a conserved 3'-to-5' exonuclease. In , it has been demonstrated that Orn plays a major role in the hydrolysis of pGpG, which is required for cyclic-di-GMP homeostasis. Meanwhile, Orn is involved in the degradation of nanoRNAs, which can alter global gene expression by serving as transcription initiation primers. Previously, we found that Orn is required for the type III secretion system and pathogenesis of , indicating a role of Orn in the bacterial response to environmental stimuli. Here we report that Orn is required for the tolerance of to ciprofloxacin. Transcriptome analysis of an mutant revealed the upregulation of pyocin biosynthesis genes. Mutation of genes involved in pyocin biosynthesis in the background of an mutant restored bacterial tolerance to ciprofloxacin. We further demonstrate that the upregulation of pyocin biosynthesis genes is due to RecA-mediated autoproteolysis of PrtR, which is the major negative regulator of pyocin biosynthesis genes. In addition, the SOS response genes were upregulated in the mutant, indicating a DNA damage stress. Therefore, our results revealed a novel role of Orn in bacterial tolerance to ciprofloxacin.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Tolerância a Medicamentos/genética
Exorribonucleases/genética
Regulação Bacteriana da Expressão Gênica
Pseudomonas aeruginosa/genética
Piocinas/biossíntese
Transcriptoma
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Proteínas de Bactérias/metabolismo
Ciprofloxacino/farmacologia
Exorribonucleases/metabolismo
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/crescimento & desenvolvimento
Pseudomonas aeruginosa/metabolismo
Recombinases Rec A/genética
Recombinases Rec A/metabolismo
Proteínas Repressoras/genética
Proteínas Repressoras/metabolismo
Resposta SOS (Genética)
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Pyocins); 0 (Repressor Proteins); 0 (prtR protein, Pseudomonas aeruginosa); 5E8K9I0O4U (Ciprofloxacin); EC 2.7.7.- (Rec A Recombinases); EC 3.1.- (Exoribonucleases); EC 3.1.- (oligoribonuclease)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE


  3 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27252387
[Au] Autor:McCaughey LC; Josts I; Grinter R; White P; Byron O; Tucker NP; Matthews JM; Kleanthous C; Whitchurch CB; Walker D
[Ad] Endereço:The Ithree Institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, U.K. Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of
[Ti] Título:Discovery, characterization and in vivo activity of pyocin SD2, a protein antibiotic from Pseudomonas aeruginosa.
[So] Source:Biochem J;473(15):2345-58, 2016 Aug 01.
[Is] ISSN:1470-8728
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Increasing rates of antibiotic resistance among Gram-negative pathogens such as Pseudomonas aeruginosa means alternative approaches to antibiotic development are urgently required. Pyocins, produced by P. aeruginosa for intraspecies competition, are highly potent protein antibiotics known to actively translocate across the outer membrane of P. aeruginosa. Understanding and exploiting the mechanisms by which pyocins target, penetrate and kill P. aeruginosa is a promising approach to antibiotic development. In this work we show the therapeutic potential of a newly identified tRNase pyocin, pyocin SD2, by demonstrating its activity in vivo in a murine model of P. aeruginosa lung infection. In addition, we propose a mechanism of cell targeting and translocation for pyocin SD2 across the P. aeruginosa outer membrane. Pyocin SD2 is concentrated at the cell surface, via binding to the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide (LPS), from where it can efficiently locate its outer membrane receptor FpvAI. This strategy of utilizing both the CPA and a protein receptor for cell targeting is common among pyocins as we show that pyocins S2, S5 and SD3 also bind to the CPA. Additional data indicate a key role for an unstructured N-terminal region of pyocin SD2 in the subsequent translocation of the pyocin into the cell. These results greatly improve our understanding of how pyocins target and translocate across the outer membrane of P. aeruginosa. This knowledge could be useful for the development of novel anti-pseudomonal therapeutics and will also support the development of pyocin SD2 as a therapeutic in its own right.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Pseudomonas aeruginosa/química
Piocinas/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/farmacologia
Dicroísmo Circular
Clonagem Molecular
Pneumopatias/tratamento farmacológico
Camundongos
Piocinas/química
Piocinas/farmacologia
Espalhamento a Baixo Ângulo
Espectrofotometria Ultravioleta
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Pyocins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160603
[St] Status:MEDLINE
[do] DOI:10.1042/BCJ20160470


  4 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27119970
[Au] Autor:Hocquet D; Petitjean M; Rohmer L; Valot B; Kulasekara HD; Bedel E; Bertrand X; Plésiat P; Köhler T; Pantel A; Jacobs MA; Hoffman LR; Miller SI
[Ad] Endereço:UMR CNRS 6249, Chrono-environnement, Université de Bourgogne-Franche-Comté, Besançon, France. dhocquet@chu-besancon.fr.
[Ti] Título:Pyomelanin-producing Pseudomonas aeruginosa selected during chronic infections have a large chromosomal deletion which confers resistance to pyocins.
[So] Source:Environ Microbiol;18(10):3482-3493, 2016 Oct.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:When bacterial lineages make the transition from free-living to permanent association with hosts, they can undergo massive gene losses, for which the selective forces within host tissues are unknown. We identified here melanogenic clinical isolates of Pseudomonas aeruginosa with large chromosomal deletions (66 to 270 kbp) and characterized them to investigate how they were selected. When compared with their wild-type parents, melanogenic mutants (i) exhibited a lower fitness in growth conditions found in human tissues, such as hyperosmolarity and presence of aminoglycoside antibiotics, (ii) narrowed their metabolic spectrum with a growth disadvantage with particular carbon sources, including aromatic amino acids and acyclic terpenes, suggesting a reduction of metabolic flexibility. Despite an impaired fitness in rich media, melanogenic mutants can inhibit their wild-type parents and compete with them in coculture. Surprisingly, melanogenic mutants became highly resistant to two intraspecific toxins, the S-pyocins AP41 and S1. Our results suggest that pyocins produced within a population of infecting P. aeruginosa may have selected for bacterial mutants that underwent massive gene losses and that were adapted to the life in diverse bacterial communities in the human host. Intraspecific interactions may therefore be an important factor driving the continuing evolution of pathogens during host infections.
[Mh] Termos MeSH primário: Deleção Cromossômica
Farmacorresistência Bacteriana
Melaninas/metabolismo
Infecções por Pseudomonas/microbiologia
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/metabolismo
Piocinas/farmacologia
[Mh] Termos MeSH secundário: Cromossomos Bacterianos/genética
Cromossomos Bacterianos/metabolismo
Seres Humanos
Pseudomonas aeruginosa/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Melanins); 0 (Pyocins); 0 (pyomelanin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171001
[Lr] Data última revisão:
171001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160428
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13336


  5 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27075392
[Au] Autor:Turnbull L; Toyofuku M; Hynen AL; Kurosawa M; Pessi G; Petty NK; Osvath SR; Cárcamo-Oyarce G; Gloag ES; Shimoni R; Omasits U; Ito S; Yap X; Monahan LG; Cavaliere R; Ahrens CH; Charles IG; Nomura N; Eberl L; Whitchurch CB
[Ad] Endereço:The ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
[Ti] Título:Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms.
[So] Source:Nat Commun;7:11220, 2016 Apr 14.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many bacteria produce extracellular and surface-associated components such as membrane vesicles (MVs), extracellular DNA and moonlighting cytosolic proteins for which the biogenesis and export pathways are not fully understood. Here we show that the explosive cell lysis of a sub-population of cells accounts for the liberation of cytosolic content in Pseudomonas aeruginosa biofilms. Super-resolution microscopy reveals that explosive cell lysis also produces shattered membrane fragments that rapidly form MVs. A prophage endolysin encoded within the R- and F-pyocin gene cluster is essential for explosive cell lysis. Endolysin-deficient mutants are defective in MV production and biofilm development, consistent with a crucial role in the biogenesis of MVs and liberation of extracellular DNA and other biofilm matrix components. Our findings reveal that explosive cell lysis, mediated through the activity of a cryptic prophage endolysin, acts as a mechanism for the production of bacterial MVs.
[Mh] Termos MeSH primário: Bacteriólise
Biofilmes
Membrana Celular/metabolismo
Biogênese de Organelas
Pseudomonas aeruginosa/fisiologia
[Mh] Termos MeSH secundário: Bacteriólise/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Membrana Celular/efeitos dos fármacos
DNA Bacteriano/metabolismo
Endopeptidases/farmacologia
Espaço Extracelular/metabolismo
Pseudomonas aeruginosa/efeitos dos fármacos
Piocinas/farmacologia
Quinolonas/farmacologia
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2-heptyl-3-hydroxy-4-quinolone); 0 (DNA, Bacterial); 0 (Pyocins); 0 (Quinolones); EC 3.4.- (Endopeptidases); EC 3.4.99.- (endolysin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160415
[St] Status:MEDLINE
[do] DOI:10.1038/ncomms11220


  6 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26905630
[Au] Autor:Inglis RF; Scanlan P; Buckling A
[Ad] Endereço:Department of Biology, Washington University in St Louis, St Louis, MO, USA.
[Ti] Título:Iron availability shapes the evolution of bacteriocin resistance in Pseudomonas aeruginosa.
[So] Source:ISME J;10(8):2060-6, 2016 08.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The evolution of bacterial resistance to conventional antimicrobials is a widely documented phenomenon with gravely important consequences for public health. However, bacteria also produce a vast repertoire of natural antimicrobials, presumably in order to kill competing species. Bacteriocins are a common class of protein-based antimicrobials that have been shown to have an important role in the ecology and evolution of bacterial communities. Relative to the evolution of antibiotic resistance, little is known about how novel resistance to these toxic compounds evolves. In this study, we present results illustrating that, although resistance is able to evolve, it remains critically dependent on the environmental context. Resistance to bacteriocins, in particular the pyocin S2, evolves readily when iron is present but less so when iron is limiting, because the receptor for this pyocin is also required for iron uptake during iron limitation. This suggests that although resistance to bacteriocins can easily evolve, environmental conditions will determine how and when resistance occurs.
[Mh] Termos MeSH primário: Bacteriocinas/farmacologia
Ferro/metabolismo
Pseudomonas aeruginosa/efeitos dos fármacos
[Mh] Termos MeSH secundário: Evolução Biológica
Farmacorresistência Bacteriana
Meio Ambiente
Ferro/deficiência
Pseudomonas aeruginosa/genética
Pseudomonas aeruginosa/fisiologia
Piocinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacteriocins); 0 (Pyocins); E1UOL152H7 (Iron)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2016.15


  7 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26860427
[Au] Autor:Dingemans J; Ghequire MG; Craggs M; De Mot R; Cornelis P
[Ad] Endereço:Department of Bioengineering Sciences, Research group Microbiology and VIB Department of Structural Biology, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, 1050, Belgium.
[Ti] Título:Identification and functional analysis of a bacteriocin, pyocin S6, with ribonuclease activity from a Pseudomonas aeruginosa cystic fibrosis clinical isolate.
[So] Source:Microbiologyopen;5(3):413-23, 2016 Jun.
[Is] ISSN:2045-8827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:S-type pyocins are bacteriocins produced by Pseudomonas aeruginosa isolates to antagonize or kill other strains of the same species. They have a modular organization comprising a receptor-binding domain recognizing a surface constituent of the target bacterium, a domain for translocation through the periplasm, and a killing or toxic domain with DNase, tRNase, or pore-forming activity. Pyocins S2, S3, S4, and S5 recognize TonB-dependent ferri-siderophore receptors in the outer membrane. We here describe a new nuclease bacteriocin, pyocin S6, encoded in the genome of a P. aeruginosa cystic fibrosis (CF) clinical isolate, CF_PA39. Similarly to pyocins S1 and S2, the S6 toxin-immunity gene tandem was recruited to the genomic region encoding exotoxin A. The pyocin S6 receptor-binding and translocation domains are identical to those of pyocin S1, whereas the killing domain is similar to the 16S ribonuclease domain of Escherichia coli colicin E3. The cytotoxic activity was abolished in pyocin S6 forms with a mutation in the colicin E3-equivalent catalytic motif. The CF_PA39 S6 immunity gene displays a higher expression level than the gene encoding the killing protein, the latter being only detected when bacteria are grown under iron-limiting conditions. In the S1-pyocinogenic strain P. aeruginosa ATCC 25324 and pyocin S2 producer P. aeruginosa PAO1, a remnant of the pyocin S6 killing domain and an intact S6-type immunity gene are located downstream of their respective pyocin operons. Strain PAO1 is insensitive for pyocin S6, and its S6-type immunity gene provides protection against pyocin S6 activity. Purified pyocin S6 inhibits one-fifth of 110 P. aeruginosa CF clinical isolates tested, showing clearer inhibition zones when the target cells are grown under iron limitation. In this panel, about half of the CF clinical isolates were found to host the S6 genes. The pyocin S6 locus is also present in the genome of some non-CF clinical isolates.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Fibrose Cística/microbiologia
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/patogenicidade
Piocinas/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antibacterianos/química
Proteínas da Membrana Bacteriana Externa/metabolismo
Bacteriocinas/genética
Sequência de Bases
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Seres Humanos
Testes de Sensibilidade Microbiana
Estrutura Terciária de Proteína
Pseudomonas aeruginosa/genética
Pseudomonas aeruginosa/isolamento & purificação
Piocinas/química
Piocinas/imunologia
RNA Ribossômico 16S/genética
RNA Ribossômico 16S/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Outer Membrane Proteins); 0 (Bacteriocins); 0 (Pyocins); 0 (RNA, Ribosomal, 16S); 148937-59-1 (pyocin S1)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160211
[St] Status:MEDLINE
[do] DOI:10.1002/mbo3.339


  8 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26812402
[Au] Autor:Wang D; Yu JM; Dorosky RJ; Pierson LS; Pierson EA
[Ad] Endereço:Earth and Environmental Sciences, Los Alamos National Laboratory, Los Alamos, NM, 87544, United States of America.
[Ti] Título:The Phenazine 2-Hydroxy-Phenazine-1-Carboxylic Acid Promotes Extracellular DNA Release and Has Broad Transcriptomic Consequences in Pseudomonas chlororaphis 30-84.
[So] Source:PLoS One;11(1):e0148003, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enhanced production of 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) by the biological control strain Pseudomonas chlororaphis 30-84 derivative 30-84O* was shown previously to promote cell adhesion and alter the three-dimensional structure of surface-attached biofilms compared to the wild type. The current study demonstrates that production of 2-OH-PCA promotes the release of extracellular DNA, which is correlated with the production of structured biofilm matrix. Moreover, the essential role of the extracellular DNA in maintaining the mass and structure of the 30-84 biofilm matrix is demonstrated. To better understand the role of different phenazines in biofilm matrix production and gene expression, transcriptomic analyses were conducted comparing gene expression patterns of populations of wild type, 30-84O* and a derivative of 30-84 producing only PCA (30-84PCA) to a phenazine defective mutant (30-84ZN) when grown in static cultures. RNA-Seq analyses identified a group of 802 genes that were differentially expressed by the phenazine producing derivatives compared to 30-84ZN, including 240 genes shared by the two 2-OH-PCA producing derivatives, the wild type and 30-84O*. A gene cluster encoding a bacteriophage-derived pyocin and its lysis cassette was upregulated in 2-OH-PCA producing derivatives. A holin encoded in this gene cluster was found to contribute to the release of eDNA in 30-84 biofilm matrices, demonstrating that the influence of 2-OH-PCA on eDNA production is due in part to cell autolysis as a result of pyocin production and release. The results expand the current understanding of the functions different phenazines play in the survival of bacteria in biofilm-forming communities.
[Mh] Termos MeSH primário: DNA Bacteriano/metabolismo
Pseudomonas/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Biofilmes
Perfilação da Expressão Gênica
Família Multigênica
Fenazinas/química
Fenazinas/isolamento & purificação
Fenazinas/metabolismo
Pseudomonas/genética
Pseudomonas/fisiologia
Piocinas/metabolismo
RNA/química
RNA/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de RNA
Espectrofotometria
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Phenazines); 0 (Pyocins); 2538-68-3 (1-phenazinecarboxylic acid); 63231-63-0 (RNA)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160204
[Lr] Data última revisão:
160204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160127
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0148003


  9 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26708985
[Au] Autor:Godino A; Príncipe A; Fischer S
[Ad] Endereço:Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36-Km 601-5800, Río Cuarto, Córdoba, Argentina. Electronic address: agodino@exa.unrc.edu.ar.
[Ti] Título:A ptsP deficiency in PGPR Pseudomonas fluorescens SF39a affects bacteriocin production and bacterial fitness in the wheat rhizosphere.
[So] Source:Res Microbiol;167(3):178-89, 2016 Apr.
[Is] ISSN:1769-7123
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Pseudomonas fluorescens SF39a is a plant-growth-promoting bacterium isolated from wheat rhizosphere. In this report, we demonstrate that this native strain secretes bacteriocins that inhibit growth of phytopathogenic strains of the genera Pseudomonas and Xanthomonas. An S-type pyocin gene was detected in the genome of strain SF39a and named pys. A non-polar pys::Km mutant was constructed. The bacteriocin production was impaired in this mutant. To identify genes involved in bacteriocin regulation, random transposon mutagenesis was carried out. A miniTn5Km1 mutant, called P. fluorescens SF39a-451, showed strongly reduced bacteriocin production. This phenotype was caused by inactivation of the ptsP gene which encodes a phosphoenolpyruvate phosphotransferase (EI(Ntr)) of the nitrogen-related phosphotransferase system (PTS(Ntr)). In addition, this mutant showed a decrease in biofilm formation and protease production, and an increase in surface motility and pyoverdine production compared with the wild-type strain. Moreover, we investigated the ability of strain SF39a-451 to colonize the wheat rhizosphere under greenhouse conditions. Interestingly, the mutant was less competitive than the wild-type strain in the rhizosphere. To our knowledge, this study provides the first evidence of both the relevance of the ptsP gene in bacteriocin production and functional characterization of a pyocin S in P. fluorescens.
[Mh] Termos MeSH primário: Fosfotransferases/metabolismo
Pseudomonas fluorescens/enzimologia
Pseudomonas fluorescens/metabolismo
Piocinas/metabolismo
Microbiologia do Solo
Triticum/microbiologia
[Mh] Termos MeSH secundário: Elementos de DNA Transponíveis
Deleção de Genes
Mutagênese Insercional
Fosfotransferases/genética
Pseudomonas fluorescens/genética
Rizosfera
Xanthomonas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (Pyocins); EC 2.7.- (Phosphotransferases)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151229
[St] Status:MEDLINE


  10 / 330 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:26276409
[Au] Autor:France MT; Remold SK
[Ad] Endereço:Department of Biological Sciences, University of Idaho, 875 Perimeter Drive MS 3051, Moscow, ID, 83844, USA. michael.t.france@gmail.com.
[Ti] Título:Interference Competition Among Household Strains of Pseudomonas.
[So] Source:Microb Ecol;72(4):821-830, 2016 11.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial species exhibit biogeographical patterns like those observed in larger organisms. The distribution of bacterial species is driven by environmental selection through abiotic and biotic factors as well dispersal limitations. We asked whether interference competition, a biotic factor, could explain variability in habitat use by Pseudomonas species in the human home. To answer this question, we screened almost 8000 directional, pairwise interactions between 89 Pseudomonas strains including members of the Pseudomonas aeruginosa (n = 29), Pseudomonas fluorescens (n = 21), and Pseudomonas putida (n = 39) species groups for the presence of killing. This diverse set of Pseudomonas strains includes those isolated from several different habitats within the home environment and includes combinations of strains that were isolated from different spatial scales. The use of this strain set not only allowed us to analyze the commonality and phylogenetic scale of interference competition within the genus Pseudomonas but also allowed us to investigate the influence of spatial scale on this trait. Overall, the probability of killing was found to decrease with increasing phylogenetic distance, making it unlikely that interference competition accounts for previously observed differential habitat use among Pseudomonas species and species groups. Strikingly, conspecific P. aeruginosa killing accounted for the vast majority of the observed killing, and this killing was found to differ across the habitat type and spatial scale of the strains' isolation. These data suggest that interference competition likely plays a large role in the within-species dynamics of P. aeruginosa but not other household Pseudomonas species.
[Mh] Termos MeSH primário: Interações Microbianas/fisiologia
Pseudomonas aeruginosa/crescimento & desenvolvimento
Pseudomonas fluorescens/crescimento & desenvolvimento
Pseudomonas putida/crescimento & desenvolvimento
Distribuição Espacial da População
[Mh] Termos MeSH secundário: Bacteriocinas/metabolismo
Ecossistema
Seres Humanos
Filogenia
Pseudomonas aeruginosa/classificação
Pseudomonas aeruginosa/isolamento & purificação
Pseudomonas fluorescens/classificação
Pseudomonas fluorescens/isolamento & purificação
Pseudomonas putida/classificação
Pseudomonas putida/isolamento & purificação
Piocinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Bacteriocins); 0 (Pyocins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150816
[St] Status:MEDLINE



página 1 de 33 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde