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[PMID]:29036223
[Au] Autor:Asano T; Ito H; Kariya Y; Hoshi K; Yoshihara A; Ugawa Y; Sekine H; Hirohata S; Yamaguchi Y; Sato S; Kobayashi H; Migita K; Ohira H; Hashimoto Y; Watanabe H
[Ad] Endereço:Department of Rheumatology, School of Medicine, Fukushima Medical University, Fukushima, Japan.
[Ti] Título:Evaluation of blood-brain barrier function by quotient alpha2 macroglobulin and its relationship with interleukin-6 and complement component 3 levels in neuropsychiatric systemic lupus erythematosus.
[So] Source:PLoS One;12(10):e0186414, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although quotient of alpha2 macroglobulin (Qα2MG) was previously reported to be useful for the evaluation of blood-brain barrier (BBB) function, it is not commonly used. We therefore evaluated BBB function among the various subsets of neuropsychiatric systemic lupus erythematosus (NPSLE) using quotient Q α2MG. Furthermore, we determined the correlation between Q α2MG and cerebrospinal (CSF) interleukin (IL)-6 level and quotient complement component 3 (Q C3). To determine intrathecal production of C3, the C3 index (Q C3/Q α2MG) was also calculated. Fifty-six patients with SLE were included in this study. Of these, 48 were diagnosed with NPSLE, consisting of 30 diffuse NPSLE patients (acute confusional state (ACS): n = 14, non-ACS: n = 16) and 18 patients with focal NPSLE. CSF IL-6 concentration, and paired serum and CSF levels of α2MG and C3, were measured by enzyme-linked immuno solvent assay (ELISA). The Q α2MG, Q C3, and C3 index were then calculated. Q α2MG, Q C3, and IL-6 concentrations in the CSF were significantly elevated in NPSLE compared with non-NPSLE. Among the subsets of NPSLE, significant increases in Q α2MG, CSF IL-6, and Q C3 were observed in ACS compared with non-ACS or focal NPSLE. There was a positive correlation between CSF IL-6 level and Q α2MG, as well as between Q C3 and Q α2MG, in diffuse NPSLE. There were no significant differences in C3 index between NPSLE and non-NPSLE, as well as among the subgroups of NPSLE. Our study suggests that BBB disruption is present in ACS, and elevated levels of IL-6 and C3 in CSF in diffuse NPSLE, especially in ACS, might result from their entry to the CSF from the systemic circulation through the damaged BBB, as well as increased intrathecal production. Furthermore, Q α2MG might be useful for the evaluation of BBB integrity.
[Mh] Termos MeSH primário: Barreira Hematoencefálica/metabolismo
Complemento C3/líquido cefalorraquidiano
Interleucina-6/líquido cefalorraquidiano
Vasculite Associada ao Lúpus do Sistema Nervoso Central/líquido cefalorraquidiano
alfa-Macroglobulinas/líquido cefalorraquidiano
[Mh] Termos MeSH secundário: Adulto
Estudos de Casos e Controles
Feminino
Glucose/líquido cefalorraquidiano
Seres Humanos
Vasculite Associada ao Lúpus do Sistema Nervoso Central/sangue
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (C3 protein, human); 0 (Complement C3); 0 (Interleukin-6); 0 (alpha-Macroglobulins); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186414


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[PMID]:28705706
[Au] Autor:Tanaka M; Kohashi K; Kushitani K; Yoshida M; Kurihara S; Kawashima M; Ueda Y; Souzaki R; Kinoshita Y; Oda Y; Takeshima Y; Hiyama E; Taguchi T; Tanaka Y
[Ad] Endereço:Department of Pathology, Kanagawa Children's Medical Center, Yokohama 232-8555, Japan. Electronic address: mio@zc4.so-net.ne.jp.
[Ti] Título:Inflammatory myofibroblastic tumors of the lung carrying a chimeric A2M-ALK gene: report of 2 infantile cases and review of the differential diagnosis of infantile pulmonary lesions.
[So] Source:Hum Pathol;66:177-182, 2017 Aug.
[Is] ISSN:1532-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We report 2 infantile cases of pulmonary tumor carrying a chimeric A2M-ALK gene. A2M-ALK is a newly identified anaplastic lymphoma kinase (ALK)-related chimeric gene from a tumor diagnosed as fetal lung interstitial tumor (FLIT). FLIT is a recently recognized infantile pulmonary lesion defined as a mass-like lesion that morphologically resembles the fetal lung. Grossly, FLIT characteristically appears as a well-circumscribed spongy mass, whereas the tumors in these patients were solid and firm. Histologically, the tumors showed intrapulmonary lesions composed of densely proliferating polygonal or spindle-shaped mesenchymal cells with diffuse and dense infiltrations of inflammatory cells forming microcystic or micropapillary structures lined by thyroid transcription factor 1-positive pneumocytes, favoring inflammatory myofibroblastic tumor rather than FLIT. The proliferating cells were immunoreactive for ALK, and A2M-ALK was identified in both tumors with reverse-transcription polymerase chain reaction. The dense infiltration of inflammatory cells, immunoreactivity for ALK, and identification of an ALK-related chimeric gene suggested a diagnosis of inflammatory myofibroblastic tumor. Histologically, most reported FLITs show sparse inflammatory infiltrates and a relatively low density of interstitial cells in the septa, although prominent infiltration of inflammatory cells and high cellularity of interstitial cells are seen in some FLITs. The present cases suggest that ALK rearrangements, including the chimeric A2M-ALK gene, may be present in these infantile pulmonary lesions, especially those with inflammatory cell infiltration. We propose that these infantile pulmonary lesions containing a chimeric A2M-ALK gene be categorized as a specific type of inflammatory myofibroblastic tumor that develops exclusively in neonates and infants.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/genética
Inflamação/genética
Neoplasias Pulmonares/genética
Miofibroblastos
Proteínas de Fusão Oncogênicas/genética
Receptores Proteína Tirosina Quinases/genética
alfa-Macroglobulinas/genética
[Mh] Termos MeSH secundário: Biomarcadores Tumorais/análise
Biópsia
Proliferação Celular
Diagnóstico Diferencial
Seres Humanos
Imuno-Histoquímica
Hibridização in Situ Fluorescente
Lactente
Inflamação/enzimologia
Inflamação/patologia
Inflamação/cirurgia
Neoplasias Pulmonares/enzimologia
Neoplasias Pulmonares/patologia
Neoplasias Pulmonares/cirurgia
Masculino
Miofibroblastos/enzimologia
Miofibroblastos/patologia
Valor Preditivo dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Oncogene Proteins, Fusion); 0 (alpha-Macroglobulins); EC 2.7.10.1 (A2M-ALK fusion protein, human); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE


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[PMID]:28683140
[Au] Autor:Hollmann AK; Dammann I; Wemheuer WM; Wemheuer WE; Chilla A; Tipold A; Schulz-Schaeffer WJ; Beck J; Schütz E; Brenig B
[Ad] Endereço:University of Goettingen, Institute of Veterinary Medicine, Goettingen, Germany.
[Ti] Título:Morgagnian cataract resulting from a naturally occurring nonsense mutation elucidates a role of CPAMD8 in mammalian lens development.
[So] Source:PLoS One;12(7):e0180665, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To investigate the genetic basis of hereditary lens opacities we analyzed 31 cases of bilateral congenital cataract in Red Holstein Friesian cattle. A genome-wide association study revealed a significant association on bovine chromosome 7 at positions 6,166,179 and 12,429,691. Whole genome re-sequencing of one case and four relatives showed a nonsense mutation (g.5995966C>T) in the PZP-like, alpha-2-macroglobulin domain containing 8 (CPAMD8) gene leading to a premature stop codon (CPAMD8 p.Gln74*) associated with cataract development in cattle. With immunohistochemistry we confirmed a physiological expression of CPAMD8 in the ciliary body epithelium of the eye in unaffected cattle, while the protein was not detectable in the ciliary body of cattle with cataracts. RNA expression of CPAMD8 was detected in healthy adult, fetal and cataractous lenses.
[Mh] Termos MeSH primário: Catarata/veterinária
Códon sem Sentido
Cristalino/crescimento & desenvolvimento
alfa-Macroglobulinas/fisiologia
[Mh] Termos MeSH secundário: Animais
Catarata/genética
Bovinos
Mapeamento Cromossômico
Feminino
Imuno-Histoquímica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon, Nonsense); 0 (alpha-Macroglobulins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180665


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[PMID]:28253193
[Au] Autor:Goulas T; Garcia-Ferrer I; Marrero A; Marino-Puertas L; Duquerroy S; Gomis-Rüth FX
[Ad] Endereço:.
[Ti] Título:Structural and functional insight into pan-endopeptidase inhibition by α2-macroglobulins.
[So] Source:Biol Chem;398(9):975-994, 2017 Aug 28.
[Is] ISSN:1437-4315
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Peptidases must be exquisitely regulated to prevent erroneous cleavage and one control is provided by protein inhibitors. These are usually specific for particular peptidases or families and sterically block the active-site cleft of target enzymes using lock-and-key mechanisms. In contrast, members of the +1400-residue multi-domain α2-macroglobulin inhibitor family (α2Ms) are directed against a broad spectrum of endopeptidases of disparate specificities and catalytic types, and they inhibit their targets without disturbing their active sites. This is achieved by irreversible trap mechanisms resulting from large conformational rearrangement upon cleavage in a promiscuous bait region through the prey endopeptidase. After decades of research, high-resolution structural details of these mechanisms have begun to emerge for tetrameric and monomeric α2Ms, which use 'Venus-flytrap' and 'snap-trap' mechanisms, respectively. In the former, represented by archetypal human α2M, inhibition is exerted through physical entrapment in a large cage, in which preys are still active against small substrates and inhibitors that can enter the cage through several apertures. In the latter, represented by a bacterial α2M from Escherichia coli, covalent linkage and steric hindrance of the prey inhibit activity, but only against very large substrates.
[Mh] Termos MeSH primário: Endopeptidases/metabolismo
Inibidores de Proteases/química
Inibidores de Proteases/farmacologia
alfa-Macroglobulinas/química
alfa-Macroglobulinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Endopeptidases/química
Seres Humanos
Multimerização Proteica
Estrutura Quaternária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Protease Inhibitors); 0 (alpha-Macroglobulins); EC 3.4.- (Endopeptidases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE


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[PMID]:28249975
[Au] Autor:Sousa R; Serrano P; Gomes Dias J; Oliveira JC; Oliveira A
[Ad] Endereço:Centro Hospitalar do Porto, Hospital de Santo António, Largo Professor Abel Salazar; 4099-001 Porto, Portugal.
[Ti] Título:Improving the accuracy of synovial fluid analysis in the diagnosis of prosthetic joint infection with simple and inexpensive biomarkers: C-reactive protein and adenosine deaminase.
[So] Source:Bone Joint J;99-B(3):351-357, 2017 Mar.
[Is] ISSN:2049-4408
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The aims of this study were to increase the diagnostic accuracy of the analysis of synovial fluid in the differentiation of prosthetic joint infection (PJI) by the addition of inexpensive biomarkers such as the levels of C-reactive protein (CRP), adenosine deaminase (ADA), alpha-2-macrogloblulin (α2M) and procalcitonin. PATIENTS AND METHODS: Between January 2013 and December 2015, synovial fluid and removed implants were requested from 143 revision total joint arthroplasties. A total of 55 patients met inclusion criteria of the receipt of sufficient synovial fluid, tissue samples and removed implants for analysis. The diagnosis of PJI followed the definition from a recent International Consensus Meeting to create two groups of patients; septic and aseptic. Using receiver operating characteristic curves we determined the cutoff values and diagnostic accuracy for each marker. RESULTS: There were 23 PJIs and 32 patients with aseptic loosening. The levels of total leucocyte count, proportion of polymorphonuclear leucocytes (PMNs), CRP, ADA and α2M in the synovial fluid were all significantly higher in those with a PJI than in those with aseptic loosening. The levels of procalcitonin were comparable in the two groups. Cutoff values for the optimal performance in the diagnosis of infection were: total leucocyte count > 1463 cells/µL (sensitivity (Sens) 100%, specificity (Spec) 71.9%, positive predictive value (PPV) 71.9%, negative predictive value (NPV) 100%); proportion of PMNs > 81% (Sens 78.3%, Spec 75.0%, PPV 69.2%, NPV 82.8%); CRP > 6.7mg/L (Sens 78.3%, Spec 93.8%, PPV 90.0%, NPV 85.7%); ADA > 61U/L (Sens 78.3%, Spec 96.9%, PPV 94.7%, NPV 86.1%) and α2M > 958 mg/L (Sens 47.8%, Spec 96.9%, PPV 91.7%, NPV 72.1%). The addition of a raised level of CRP or ADA to the total leukocyte count increased the specificity: total leukocyte count > 1463 cells/µL and CRP > 6.7mg/L (Sens 78.3%, Spec 100%, PPV 100%, NPV 86.5%) or with ADA > 61U/L (Sens 78.3%, Spec 96.9%, PPV 94.7%, NPV 86.1%). CONCLUSION: The total leucocyte count in the synovial fluid offers great negative predictive value in the diagnosis of PJI and the addition of more specific markers such as CRP and ADA improves the positive predictive value. Thus the addition of simple and inexpensive markers to the measurement of the leucocyte count in the synovial fluid may reduce the number of equivocal results which demand more expensive investigation. Cite this article: 2017;99-B:351-7.
[Mh] Termos MeSH primário: Prótese de Quadril/efeitos adversos
Prótese do Joelho/efeitos adversos
Infecções Relacionadas à Prótese/diagnóstico
Líquido Sinovial/química
[Mh] Termos MeSH secundário: Adenosina Desaminase/análise
Idoso
Artroplastia de Quadril
Artroplastia do Joelho
Biomarcadores/análise
Proteína C-Reativa/análise
Calcitonina/análise
Feminino
Seres Humanos
Contagem de Leucócitos
Masculino
Meia-Idade
Valor Preditivo dos Testes
Cuidados Pré-Operatórios/métodos
Estudos Prospectivos
Falha de Prótese/etiologia
Infecções Relacionadas à Prótese/cirurgia
Curva ROC
Reoperação
Sensibilidade e Especificidade
Líquido Sinovial/citologia
alfa-Macroglobulinas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (A2M protein, human); 0 (Biomarkers); 0 (alpha-Macroglobulins); 9007-12-9 (Calcitonin); 9007-41-4 (C-Reactive Protein); EC 3.5.4.4 (Adenosine Deaminase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1302/0301-620X.99B3.BJJ-2016-0684.R1


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[PMID]:28215695
[Au] Autor:Huangfu C; Ma Y; Jia J; Lv M; Zhu F; Ma X; Zhao X; Zhang J
[Ad] Endereço:Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
[Ti] Título:Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-Macroglobulin from Cohn Fraction IV.
[So] Source:Biologicals;46:139-142, 2017 Mar.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5.88 log , 7.50 log , 4.88 log , and 5.63 log respectively within 2 h. By pasteurization with 40% glucose vesicular stomatitis virus was inactivated more than 5.88 log within 1 h. Only 2.71 log reduction was achieved for encephalomyocarditis virus after 10 h. 40% glucose protected α2-M activity and viruses simultaneously from pasteurization. Other viral inactivation methods need to be incorporated to ensure viral safety of this manufacturing process of α2-Macroglobulin.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/metabolismo
Glucose/farmacologia
Temperatura Alta
Pasteurização/métodos
Inativação de Vírus/efeitos dos fármacos
alfa-Macroglobulinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Vírus da Encefalomiocardite/fisiologia
Herpesvirus Suídeo 1/fisiologia
Seres Humanos
Parvovirus Suíno/fisiologia
Reprodutibilidade dos Testes
Vírus Sindbis/fisiologia
Suínos
Fatores de Tempo
Células Vero
Vírus da Estomatite Vesicular Indiana/efeitos dos fármacos
Vírus da Estomatite Vesicular Indiana/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (Cohn fraction IV); 0 (alpha-Macroglobulins); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


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[PMID]:28124491
[Au] Autor:Appeltant R; Beek J; Maes D; Bijttebier J; Van Steendam K; Nauwynck H; Van Soom A
[Ad] Endereço:Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki, Japan.
[Ti] Título:Hampered cumulus expansion of porcine cumulus-oocyte complexes by excessive presence of alpha -macroglobulin is likely mediated via inhibition of zinc-dependent metalloproteases.
[So] Source:Anim Sci J;88(9):1279-1290, 2017 Sep.
[Is] ISSN:1740-0929
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:In vitro maturation (IVM) in serum causes hampered expansion of porcine cumulus-oocyte complexes (COCs) due to excessive alpha -macroglobulin (A2M). This study investigated two hypotheses that could explain the effect of A2M: (i) binding of epidermal growth factor (EGF) to A2M, followed by its decreased availability; and (ii) inhibition of zinc-dependent metalloproteases. Cumulus expansion was evaluated based on the diameter of the COCs, the proportion of COCs participating in a floating cloud and the proportion of COCs with loss of cumulus cells. The first hypothesis of decreased EGF availability was tested by increasing the EGF concentration (20 and 50 ng/mL vs. 10 ng/mL), but was not confirmed because cumulus expansion did not improve. To verify the second hypothesis of inhibited zinc-dependent metalloproteases, the effect of tissue inhibitor of metalloproteases-3 (TIMP-3) on cumulus expansion during IVM with and without A2M was investigated. To immuno-neutralize A2M, serum was pre-incubated with A2M antibodies. Impaired cumulus expansion because of TIMP-3 could only be observed during IVM in 10% of serum with A2M antibodies. No effect of TIMP-3 was observed in medium without A2M antibodies. These results indicate that A2M and TIMP-3 share a common target, a zinc-dependent metalloprotease. Future research is directed toward the identification of the protease involved.
[Mh] Termos MeSH primário: Técnicas de Maturação in Vitro de Oócitos
Metaloproteases/antagonistas & inibidores
Oócitos/fisiologia
Zinco
alfa-Macroglobulinas/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Suínos
Inibidor Tecidual de Metaloproteinase-3/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tissue Inhibitor of Metalloproteinase-3); 0 (alpha-Macroglobulins); EC 3.4.- (Metalloproteases); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1111/asj.12767


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[PMID]:28106476
[Au] Autor:Delaby C; Bros P; Vialaret J; Moulinier A; Delatour V; Gabelle A; Lehmann S; Hirtz C
[Ad] Endereço:Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.
[Ti] Título:Quantification of hepcidin-25 in human cerebrospinal fluid using LC-MS/MS.
[So] Source:Bioanalysis;9(4):337-347, 2017 Feb.
[Is] ISSN:1757-6199
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: Hepcidin, the main iron metabolism regulator, can be detected in various biological fluids. Here, we describe a quantitative method of LC-MS/MS to quantify the 25 amino acid isoform of hepcidin (hepcidin-25) in human cerebrospinal fluid (CSF). Results & methodology: Samples were prepared through protein precipitation followed by solid phase extraction (SPE) and injected into a triple-quadrupole mass spectrometer. Validation of our method included determination of LOQ (0.1 ng/ml), repeatability, intermediate precision, recovery and linearity (up to 25 ng/ml). Hepcidin-25 was subsequently quantified in 36 human CSF samples and its concentration ranged from 0.21 to 3.54 ng/ml. CONCLUSION: This is the first time that hepcidin-25 can be reliably quantified in human CSF. This may open interesting perspectives for the management of iron-related neurological disorders.
[Mh] Termos MeSH primário: Cromatografia Líquida
Hepcidinas/líquido cefalorraquidiano
Espectrometria de Massas em Tandem
[Mh] Termos MeSH secundário: Animais
Cabras
Hepcidinas/sangue
Seres Humanos
Ferro/química
Limite de Detecção
Modelos Lineares
Doenças do Sistema Nervoso/líquido cefalorraquidiano
Doenças do Sistema Nervoso/diagnóstico
Reprodutibilidade dos Testes
Extração em Fase Sólida
alfa-Macroglobulinas/líquido cefalorraquidiano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hepcidins); 0 (alpha-Macroglobulins); 0 (hepcidin 25, human); E1UOL152H7 (Iron)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.4155/bio-2016-0240


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[PMID]:28087279
[Au] Autor:Li J; Liu X; Xiang Y; Ding X; Wang T; Liu Y; Yin M; Tan C; Deng F; Chen L
[Ad] Endereço:Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China.
[Ti] Título:Alpha-2-macroglobulin and heparin cofactor II and the vulnerability of carotid atherosclerotic plaques: An iTRAQ-based analysis.
[So] Source:Biochem Biophys Res Commun;483(3):964-971, 2017 Feb 12.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rupture of carotid atherosclerotic plaque may cause stroke, while few biomarker in clinic can evaluate carotid plaque vulnerability. In this study, we divided the recruited participants into no plaque, stable plaque, and vulnerable plaque group according to carotid ultrasonography, and screened the differentially expressed proteins in plasma of these participants using isobaric tags for relative and absolute quantitation labeling coupled with liquid chromatography-tandem mass spectrometry. 28 proteins were identified differentially expressed, among which alpha-2-macroglobulin (α2M) and heparin cofactor II (HCII) were found to be at hub position in the interactions of these proteins by STRING analysis and were selected for enzyme-linked immunosorbent assay measurement to assess their relevance with carotid plaques vulnerability and diagnostic efficiency. The plasma level of α2M was found positively correlated, while HCII level was negatively correlated with higher vulnerability of carotid plaques. Both proteins were efficient in differentiating stable and vulnerable carotid plaques. These findings provide potential new targets for the research of carotid plaque vulnerability. Plasma α2M and HCII may be potential biomarkers for evaluation of the vulnerability of carotid plaques if further studied.
[Mh] Termos MeSH primário: Estenose das Carótidas/sangue
Cofator II da Heparina/metabolismo
Placa Aterosclerótica/sangue
alfa-Macroglobulinas/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores/sangue
Análise Química do Sangue/métodos
Feminino
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (A2M protein, human); 0 (Biomarkers); 0 (SERPIND1 protein, human); 0 (alpha-Macroglobulins); 81604-65-1 (Heparin Cofactor II)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE


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[PMID]:28027986
[Au] Autor:Ponprateep S; Vatanavicharn T; Lo CF; Tassanakajon A; Rimphanitchayakit V
[Ad] Endereço:Department of Chemistry, Faculty of Science, Srinakharinwirot University, 114 Sukhumvit 23 Road, Bangkok 10110, Thailand. Electronic address: sirikwanp@g.swu.ac.th.
[Ti] Título:Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai.
[So] Source:Fish Shellfish Immunol;62:68-74, 2017 Mar.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The shrimp multifunctional protein alpha-2-macroglobulin (A2M) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. Herein, the function of LvA2M from Litopenaeus vannamei on the prophenoloxidase (proPO) system is reported. The recombinant (r)LvA2M produced strongly and specifically inhibited trypsin and the PO activity in shrimp plasma in a dose-dependent manner. Silencing of LvA2M led to an increase in the PO activity in shrimp plasma although the expression of proPO-associated genes, proPO-activating enzyme (PPAE) and prophenoloxidase (proPO) but not the proPO-activating factor (PPAF) was down-regulated. In Vibrio parahaemolyticus AHPND-infected shrimp, the LvA2M activity was suppressed in an early phase of infection while the PO activity was increased. Thus, the proPO-activating system was regulated by the LvA2M.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/genética
Imunidade Inata
Penaeidae/genética
Vibrio parahaemolyticus/fisiologia
alfa-Macroglobulinas/genética
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/metabolismo
Catecol Oxidase/genética
Catecol Oxidase/metabolismo
Precursores Enzimáticos/genética
Precursores Enzimáticos/metabolismo
Expressão Gênica
Técnicas de Silenciamento de Genes
Penaeidae/imunologia
Penaeidae/microbiologia
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Serina Proteases/genética
Serina Proteases/metabolismo
alfa-Macroglobulinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Enzyme Precursors); 0 (Recombinant Proteins); 0 (alpha-Macroglobulins); EC 1.10.3.- (pro-phenoloxidase); EC 1.10.3.1 (Catechol Oxidase); EC 3.4.- (Serine Proteases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE



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