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  1 / 2025 MEDLINE  
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[PMID]:28467300
[Au] Autor:Ku AT; Shaver TM; Rao AS; Howard JM; Rodriguez CN; Miao Q; Garcia G; Le D; Yang D; Borowiak M; Cohen DN; Chitsazzadeh V; Diwan AH; Tsai KY; Nguyen H
[Ad] Endereço:Stem Cell and Regenerative Medicine Center, Baylor College of Medicine, Houston, United States.
[Ti] Título:TCF7L1 promotes skin tumorigenesis independently of ß-catenin through induction of LCN2.
[So] Source:Elife;6, 2017 05 03.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The transcription factor is an embryonic stem cell signature gene that is upregulated in multiple aggressive cancer types, but its role in skin tumorigenesis has not yet been defined. Here we document TCF7L1 upregulation in skin squamous cell carcinoma (SCC) and demonstrate that TCF7L1 overexpression increases tumor incidence, tumor multiplicity, and malignant progression in the chemically induced mouse model of skin SCC. Additionally, we show that downregulation of TCF7L1 and its paralogue TCF7L2 reduces tumor growth in a xenograft model of human skin SCC. Using separation-of-function mutants, we show that TCF7L1 promotes tumor growth, enhances cell migration, and overrides oncogenic RAS-induced senescence independently of its interaction with ß-catenin. Through transcriptome profiling and combined gain- and loss-of-function studies, we identified LCN2 as a major downstream effector of TCF7L1 that drives tumor growth. Our findings establish a tumor-promoting role for TCF7L1 in skin and elucidate the mechanisms underlying its tumorigenic capacity.
[Mh] Termos MeSH primário: Carcinogênese
Carcinoma de Células Escamosas/fisiopatologia
Lipocalina-2/metabolismo
Neoplasias Cutâneas/fisiopatologia
Proteína 1 Semelhante ao Fator 7 de Transcrição/metabolismo
beta Catenina/metabolismo
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Perfilação da Expressão Gênica
Xenoenxertos
Seres Humanos
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (CTNNB1 protein, human); 0 (LCN2 protein, human); 0 (Lipocalin-2); 0 (TCF7L1 protein, human); 0 (Transcription Factor 7-Like 1 Protein); 0 (beta Catenin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  2 / 2025 MEDLINE  
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[PMID]:29355013
[Au] Autor:Zhang W; Li X; Hua F; Chen W; Wang W; Chu GX; Bao GH
[Ad] Endereço:Natural Products Laboratory, International Joint Lab of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, 230036 People's Republic of China.
[Ti] Título:Interaction between Ester-Type Tea Catechins and Neutrophil Gelatinase-Associated Lipocalin: Inhibitory Mechanism.
[So] Source:J Agric Food Chem;66(5):1147-1156, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tea is thought to alleviate neurotoxicity due to the antioxidative effect of ester-type tea catechins (ETC). Neutrophil gelatinase-associated lipocalin (NGAL) can sensitize ß-amyloid (Aß) induced neurotoxicity, and inhibitors of NGAL may relieve associated symptoms. As such, the interactions of ETC with NGAL were investigated by fluorescence spectrometry and molecular simulation. NGAL fluorescence is quenched regularly when being added with six processing types of tea infusion (SPTT) and ETC. Thermodynamic analyses suggest that ETC with more catechol moieties has a stronger binding capacity with NGAL especially in the presence of Fe . (-)-Epicatechin 3-O-caffeoate (ECC), a natural product isolated from Zijuan green tea, shows the strongest binding ability with NGAL (K = 15.21 ± 8.68 nM in the presence of Fe ). All ETC are effective in protecting nerve cells against H O or Aß induced injury. The inhibitory mechanism of ETC against NGAL supports its potential use in attenuation of neurotoxicity.
[Mh] Termos MeSH primário: Catequina/farmacologia
Lipocalina-2/farmacologia
Chá/química
[Mh] Termos MeSH secundário: Catequina/metabolismo
Interações Medicamentosas
Ésteres
Peróxido de Hidrogênio/farmacologia
Quelantes de Ferro
Lipocalina-2/antagonistas & inibidores
Lipocalina-2/metabolismo
Modelos Moleculares
Neurônios/efeitos dos fármacos
Fármacos Neuroprotetores
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Esters); 0 (Iron Chelating Agents); 0 (Lipocalin-2); 0 (Neuroprotective Agents); 0 (Tea); 8R1V1STN48 (Catechin); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05399


  3 / 2025 MEDLINE  
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[PMID]:29254288
[Au] Autor:Dyszkiewicz-Konwinska M; Bryja A; Jopek K; Budna J; Khozmi R; Jeseta M; Bukowska D; Antosik P; Bruska M; Nowicki M; Zabel M; Kempisty B
[Ad] Endereço:Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, Poznan, Poland.
[Ti] Título:Expression of genes responsible for cell morphogenesis involved in differentiation in porcine buccal pouch mucosal cells during long-term primary culture and real-time proliferation in vitro.
[So] Source:J Biol Regul Homeost Agents;31(4):855-864, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:Recently, using experimental animal model, we demonstrated that porcine buccal pouch mucosal cells reflect increased proliferation capability during primary cultivation in vitro. Although the histological structure and morphogenesis in oral cavity is well recognized, the molecular mechanisms which regulate this process still need further investigation. This study was aimed to analyze the molecular marker expression profile involved in morphogenesis and differentiation capacity of porcine buccal pouch mucosal cells during their long-term primary cultivation in vitro. The experiment was performed on buccal pouch mucosal cells isolated from 80 pubertal crossbred Landrace gilts. After collection, the cells were treated enzymatically and transferred into a primary in vitro culture (IVC) system and cultured for 30 days. The cells were collected for RNA isolation after 7, 15 and 30 days of IVC and were checked for their real-time proliferative status using the RTCA system. We found an increased expression of FN1 and SOX9 genes when calculated against ACTB after 7, and 30 days of IVC, (P less than 0.01, P less than 0.001, respectively). The CXCL12 mRNA was down-regulated after 7, 15 and 30 days of IVC, but not statistically significant. Similar expression profile was observed when calculated against HPRT, however, DAB2 was found to be higher expressed at day 15 of IVC, (P less than 0.05). The cell index measured during real-time cell proliferation was substantially increased between 96 h and 147h of IVC and reached the log phase. Since FN1 and SOX9 revealed significant increase of expression after long-term culture in vitro, it is suggested that expression of these differentiation and stemness genes is accompanied by cell proliferation. Moreover, FN1 and SOX9 might be recognized as new markers of buccal pouch mucosal cell proliferation and differentiation in pigs in in vitro primary culture model.
[Mh] Termos MeSH primário: Células Epiteliais/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Lipocalina-2/genética
Morfogênese/genética
Mucosa Bucal/metabolismo
Fatores de Transcrição SOX9/genética
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Proliferação Celular
Quimiocina CXCL12/genética
Quimiocina CXCL12/metabolismo
Células Epiteliais/citologia
Feminino
Perfilação da Expressão Gênica
Hipoxantina Fosforribosiltransferase/genética
Hipoxantina Fosforribosiltransferase/metabolismo
Lipocalina-2/metabolismo
Mucosa Bucal/citologia
Mucosa Bucal/crescimento & desenvolvimento
Cultura Primária de Células
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Fatores de Transcrição SOX9/metabolismo
Transdução de Sinais
Suínos
Proteínas Supressoras de Tumor/genética
Proteínas Supressoras de Tumor/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokine CXCL12); 0 (Lipocalin-2); 0 (RNA, Messenger); 0 (SOX9 Transcription Factor); 0 (Tumor Suppressor Proteins); EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  4 / 2025 MEDLINE  
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[PMID]:29304176
[Au] Autor:Maier HT; Ashraf MI; Denecke C; Weiss S; Augustin F; Messner F; Vallant N; Böcklein M; Margreiter C; Göbel G; Pratschke J; Öfner-Velano D; Aigner F
[Ad] Endereço:Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Innsbruck, Austria.
[Ti] Título:Prediction of delayed graft function and long-term graft survival by serum and urinary neutrophil gelatinase-associated lipocalin during the early postoperative phase after kidney transplantation.
[So] Source:PLoS One;13(1):e0189932, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neutrophil gelatinase-associated lipocalin (NGAL) has emerged as an early marker protein for kidney dysfunction in various clinical settings. In this prospective study we evaluated serial changes of serum and urinary NGAL within the first 7 days after kidney transplantation in 170 consecutive recipients. The main focus of this study was to assess the performance of serum and urinary NGAL in the prediction of delayed graft function (DGF) and two-year graft and patient survival. Serum and urine samples of 170 patients undergoing primary kidney transplantation from October 2010 to December 2012 were prospectively collected from day 0 to 7. NGAL was analyzed by ELISA. Multivariate regression models, receiver-operating characteristics (ROC), and areas under ROC curves (AUC) were used to identify predictors of DGF. DGF occurred in 52 patients (30.6%). Serum (AUC = 0.869) and urinary NGAL (AUC = 0.872) on postoperative day (POD) 2 could accurately predict DGF compared to serum creatinine (AUC = 0.619). Multivariate analyses revealed donor age, serum and urinary NGAL significantly associated with DGF (p<0.001). Recipient age was the only significant factor in a cox regression model influencing two-year graft and patient survival. In conclusion, serum and urinary NGAL are early predictors of DGF after kidney transplantation.
[Mh] Termos MeSH primário: Função Retardada do Enxerto
Sobrevivência de Enxerto
Transplante de Rim
Lipocalina-2/sangue
Lipocalina-2/urina
[Mh] Termos MeSH secundário: Adulto
Idoso
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Masculino
Meia-Idade
Período Pós-Operatório
Estudos Prospectivos
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (LCN2 protein, human); 0 (Lipocalin-2)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189932


  5 / 2025 MEDLINE  
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[PMID]:27775820
[Au] Autor:Das S; Maras JS; Hussain MS; Sharma S; David P; Sukriti S; Shasthry SM; Maiwall R; Trehanpati N; Singh TP; Sarin SK
[Ad] Endereço:Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India.
[Ti] Título:Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.
[So] Source:Hepatology;65(2):631-646, 2017 02.
[Is] ISSN:1527-3350
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Albumin is a potent scavenger of reactive oxygen species (ROS). However, modifications in albumin structure may reduce its antioxidant properties and modulate its immune-regulatory functions. We examined alterations in circulating albumin in severe alcoholic hepatitis (SAH) patients and their contribution to neutrophil activation, intracellular stress, and alteration in associated molecular pathways. Albumin modifications and plasma oxidative stress were assessed in SAH patients (n = 90), alcoholic cirrhosis patients (n = 60), and healthy controls (n = 30) using liquid chromatography/mass spectrometry and spectrophotometry. Activation and intracellular ROS were measured in healthy neutrophils after treatment with purified albumin from the study groups. Gene expression of SAH neutrophils was analyzed and compared to gene expression from healthy neutrophils after stimulation with purified albumin from SAH patient plasma. SAH-albumin showed the highest albumin oxidative state (P < 0.05) and prominent alteration as human nonmercaptalbumin 2 (P < 0.05). Plasma oxidative stress (advanced oxidative protein product) was higher in SAH versus alcoholic cirrhosis patients and healthy controls (P < 0.05). Neutrophil gelatinase-associated lipocalin, myeloperoxidase, and intracellular ROS levels were highest in SAH-albumin-treated neutrophils (P < 0.05). Genes associated with neutrophil activation, ROS production, intracellular antioxidation, and leukocyte migration plus genes for proinflammatory cytokines and various toll-like receptors were overexpressed in SAH neutrophils compared to healthy neutrophils (P < 0.05). Expression of the above-mentioned genes in SAH-albumin-stimulated healthy neutrophils was comparable with SAH patient neutrophils, except for genes associated with apoptosis, endoplasmic reticulum stress, and autophagy (P < 0.05). CONCLUSIONS: In patients with SAH, there is a significant increase in albumin oxidation, and albumin acts as a pro-oxidant; this promotes oxidative stress and inflammation in SAH patients through activation of neutrophils. (Hepatology 2017;65:631-646).
[Mh] Termos MeSH primário: Hepatite Alcoólica/sangue
Hepatite Alcoólica/patologia
Estresse Oxidativo/fisiologia
Espécies Reativas de Oxigênio/sangue
[Mh] Termos MeSH secundário: Adulto
Cromatografia Líquida
Estudos Transversais
Feminino
Seres Humanos
Lipocalina-2/metabolismo
Testes de Função Hepática
Masculino
Espectrometria de Massas
Meia-Idade
Ativação de Neutrófilo
Reação em Cadeia da Polimerase em Tempo Real/métodos
Valores de Referência
Índice de Gravidade de Doença
Estatísticas não Paramétricas
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (LCN2 protein, human); 0 (Lipocalin-2); 0 (Reactive Oxygen Species)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180121
[Lr] Data última revisão:
180121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1002/hep.28897


  6 / 2025 MEDLINE  
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[PMID]:29258482
[Au] Autor:Oh SM; Park G; Lee SH; Seo CS; Shin HK; Oh DS
[Ad] Endereço:The K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea.
[Ti] Título:Assessing the recovery from prerenal and renal acute kidney injury after treatment with single herbal medicine via activity of the biomarkers HMGB1, NGAL and KIM-1 in kidney proximal tubular cells treated by cisplatin with different doses and exposure times.
[So] Source:BMC Complement Altern Med;17(1):544, 2017 Dec 19.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Acute kidney injury (AKI) is an initial factor in many kidney disorders. Pre- and intra-renal AKI biomarkers have recently been reported. Recovery from AKI by herbal medicine has rarely been reported. Thus, this study aimed to investigate the dose- and time-dependent effects of herbal medicines to protect against AKI in cisplatin-induced human kidney 2 (HK-2) cells by assessing the activities of high-mobility group box protein 1 (HMGB1), neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1). METHODS: Proximal tubular HK-2 cell lines were treated with either 400 µM of cisplatin for 6 h or 10 µM of cisplatin for 24 h and then exposed to ten types of single herbal medicines, including Nelumbo nymphaea (NY) at a dose of 100 µg/mL. The AKI biomarkers HMGB1, NGAL and KIM-1 were repeatedly measured by an ELISA assay at 2, 4, and 6 h in the group treated with 400 µM of cisplatin to confirm necrotic cell death and at 6, 24, and 48 h in the group treated with 10 µM of cisplatin to examine apoptotic cell death. Recovery confirm was conducted through in vivo study using ICR mice for 3 day NY or Paeonia suffruticosa intake. RESULTS: Cisplatin treatment at a concentration of 10 µM decreased cell viability. Treatment with 400 µM of cisplatin reduced HMBG1 activity and resulted in lactate dehydrogenase release. In longer exposure durations (up to 48 h), NGAL and KIM-1 exhibited activity from 24 h onward. Additionally, NY treatment resulted in an approximately 50% change in all three biomarkers. The time-dependent profiles of HMGB1, NGAL and KIM-1 activities up to 48 h were notably different; HMGB1 exhibited a 7-fold change at 6 h, and NGAL and KIM-1 exhibited 1.7-fold changes at 24 h, respectively. Consistently, serum and urine NGAL and KIM-1 activities were all reduced in ICR mice. CONCLUSIONS: Several single herbal medicines, including NY, have a potential as effectors of AKI due to their ability to inhibit the activation of HMGB1, NGAL and KIM-1 in an in vitro AKI-mimicked condition and simple in vivo confirm. Furthermore, an in vivo proof-of-concept study is needed.
[Mh] Termos MeSH primário: Lesão Renal Aguda/tratamento farmacológico
Preparações de Plantas/farmacologia
Preparações de Plantas/uso terapêutico
Substâncias Protetoras/farmacologia
Substâncias Protetoras/uso terapêutico
[Mh] Termos MeSH secundário: Lesão Renal Aguda/induzido quimicamente
Lesão Renal Aguda/fisiopatologia
Animais
Morte Celular/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Cisplatino/efeitos adversos
Proteína HMGB1/metabolismo
Receptor Celular 1 do Vírus da Hepatite A/metabolismo
Seres Humanos
Lipocalina-2/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos ICR
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HAVCR1 protein, human); 0 (HMGB1 Protein); 0 (Hepatitis A Virus Cellular Receptor 1); 0 (Lipocalin-2); 0 (Plant Preparations); 0 (Protective Agents); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2055-y


  7 / 2025 MEDLINE  
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[PMID]:29175302
[Au] Autor:Muñoz D; Serrano MK; Hernandez ME; Haller R; Swanson T; Slaton JW; Sinha AA; Wilson MJ
[Ad] Endereço:Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, Mexico; Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, Mexico.
[Ti] Título:Matrix metalloproteinase and heparin-stimulated serine proteinase activities in post-prostate massage urine of men with prostate cancer.
[So] Source:Exp Mol Pathol;103(3):300-305, 2017 12.
[Is] ISSN:1096-0945
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Proteinases secreted by the prostate gland have a reproductive function in cleaving proteins in the ejaculate and in the female reproductive tract, but some may have a fundamental role in disease and pathological processes including cancer. The purpose of this study was to determine if there were differences in proteinase activities in urine samples collected following prostate massage of men positive (CaP) or negative (no evidence of malignancy, NEM) for biopsy determined prostate cancer. Matrix metalloproteinase (MMP) and serine proteinase activities were detected using protein substrate zymography. There were no differences in activities of MMP-2, proMMP-9, and MMP-9/NGAL (neutrophil gelatinase associated lipocalin) complex (gelatin substrate) in men with detected prostate cancer, although the latter two were somewhat diminished. A caseinolytic activity of about 75kDa inhibited by calcium did not differ between the NEM and CaP groups. Heparin stimulated calcium sensitive gelatinolytic activities of approximately 22, 42, and 60kDa, but did not affect activities of MMP-2, MMP-9, or the 75kDa caseinolytic activity. The 22, 42, and 60kDa activities appear to be serine proteinases since they were inhibited by benzamidine. There was a significant decrease in the 22kDa heparin-stimulated serine proteinase activity in urines of men with cancer. Proteinase expression and activities, perhaps in combination with other potential markers, may prove useful in urine for detection and evaluation of prostate cancer.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/urina
Metaloproteinase 2 da Matriz/urina
Metaloproteinase 9 da Matriz/urina
Neoplasias da Próstata/urina
Serina Proteases/urina
[Mh] Termos MeSH secundário: Idoso
Benzamidinas/administração & dosagem
Cálcio/metabolismo
Heparina/química
Seres Humanos
Lipocalina-2/urina
Masculino
Meia-Idade
Neoplasias da Próstata/enzimologia
Neoplasias da Próstata/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzamidines); 0 (Biomarkers, Tumor); 0 (LCN2 protein, human); 0 (Lipocalin-2); 9005-49-6 (Heparin); EC 3.4.- (Serine Proteases); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.35 (MMP9 protein, human); EC 3.4.24.35 (Matrix Metalloproteinase 9); KUE3ZY3J1F (benzamidine); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171214
[Lr] Data última revisão:
171214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  8 / 2025 MEDLINE  
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[PMID]:28449706
[Au] Autor:Borton MA; Sabag-Daigle A; Wu J; Solden LM; O'Banion BS; Daly RA; Wolfe RA; Gonzalez JF; Wysocki VH; Ahmer BMM; Wrighton KC
[Ad] Endereço:Department of Microbiology, The Ohio State University, 484 W. 12th Avenue, 440 Biological Sciences Building, Columbus, OH, 43210, USA.
[Ti] Título:Chemical and pathogen-induced inflammation disrupt the murine intestinal microbiome.
[So] Source:Microbiome;5(1):47, 2017 04 27.
[Is] ISSN:2049-2618
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Salmonella is one of the most significant food-borne pathogens to affect humans and agriculture. While it is well documented that Salmonella infection triggers host inflammation, the impacts on the gut environment are largely unknown. A CBA/J mouse model was used to evaluate intestinal responses to Salmonella-induced inflammation. In parallel, we evaluated chemically induced inflammation by dextran sodium sulfate (DSS) and a non-inflammation control. We profiled gut microbial diversity by sequencing 16S ribosomal ribonucleic acid (rRNA) genes from fecal and cecal samples. These data were correlated to the inflammation marker lipocalin-2 and short-chain fatty acid concentrations. RESULTS: We demonstrated that inflammation, chemically or biologically induced, restructures the chemical and microbial environment of the gut over a 16-day period. We observed that the ten mice within the Salmonella treatment group had a variable Salmonella relative abundance, with three high responding mice dominated by >46% Salmonella at later time points and the remaining seven mice denoted as low responders. These low- and high-responding Salmonella groups, along with the chemical DSS treatment, established an inflammation gradient with chemical and low levels of Salmonella having at least 3 log-fold lower lipocalin-2 concentration than the high-responding Salmonella mice. Total short-chain fatty acid and individual butyrate concentrations each negatively correlated with inflammation levels. Microbial communities were also structured along this inflammation gradient. Low levels of inflammation, regardless of chemical or biological induction, enriched for Akkermansia spp. in the Verrucomicrobiaceae and members of the Bacteroidetes family S24-7. Relative to the control or low inflammation groups, high levels of Salmonella drastically decreased the overall microbial diversity, specifically driven by the reduction of Alistipes and Lachnospiraceae in the Bacteroidetes and Firmicutes phyla, respectively. Conversely, members of the Enterobacteriaceae and Lactobacillus were positively correlated to high levels of Salmonella-induced inflammation. CONCLUSIONS: Our results show that enteropathogenic infection and intestinal inflammation are interrelated factors modulating gut homeostasis. These findings may prove informative with regard to prophylactic or therapeutic strategies to prevent disruption of microbial communities, or promote their restoration.
[Mh] Termos MeSH primário: Bactérias/classificação
Microbioma Gastrointestinal
Lipocalina-2/metabolismo
Salmonelose Animal/imunologia
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Animais
Bactérias/genética
Bactérias/isolamento & purificação
Ceco/microbiologia
DNA Bacteriano/genética
DNA Ribossômico/genética
Sulfato de Dextrana/efeitos adversos
Fezes/microbiologia
Camundongos
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Lipocalin-2); 0 (RNA, Ribosomal, 16S); 126469-30-5 (Lcn2 protein, mouse); 9042-14-2 (Dextran Sulfate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s40168-017-0264-8


  9 / 2025 MEDLINE  
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[PMID]:27777380
[Au] Autor:Halade GV; Kain V; Black LM; Prabhu SD; Ingle KA
[Ad] Endereço:Division of Cardiovascular Disease, Department of Medicine, The University of Alabama at Birmingham, AL 35233, USA.
[Ti] Título:Aging dysregulates D- and E-series resolvins to modulate cardiosplenic and cardiorenal network following myocardial infarction.
[So] Source:Aging (Albany NY);8(11):2611-2634, 2016 Oct 18.
[Is] ISSN:1945-4589
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Post-myocardial infarction (MI), overactive inflammation is the hallmark of aging, however, the mechanism is unclear. We hypothesized that excess influx of omega 6 fatty acids may impair resolution, thus impacting the cardiosplenic and cardiorenal network post-MI. Young and aging mice were fed on standard lab chow (LC) and excess fatty acid (safflower oil; SO)-enriched diet for 2 months and were then subjected to MI surgery. Despite similar infarct areas and left ventricle (LV) dysfunction post-MI, splenic mass spectrometry data revealed higher levels of arachidonic acid (AA) derived pro-inflammatory metabolites in young-SO, but minimal formation of docosanoids, D- and E- series resolvins in SO-fed aged mice. The aged mice receiving excess intake of fatty acids exhibit; 1) decreased lipoxygenases (5-,12-, and 15) in the infarcted LV; 2) lower levels of 14HDHA, RvD1, RvD5, protectin D1, 7(S)maresin1, 8-,11-,18-HEPE and RvE3 with high levels of tetranor-12-HETEs; 3) dual population of macrophages (CD11b /F480 and CD11b /F480 ) with increased pro-inflammatory (CD11bp F4/80 Ly6C ) phenotype and; 4) increased kidney injury marker NGAL with increased expression of TNF-α and IL-1ß indicating MI-induced non-resolving response compared with LC-group. Thus, excess fatty acid intake magnifies the post-MI chemokine signaling and inflames the cardiosplenic and cardiorenal network towards a non-resolving microenvironment in aging.
[Mh] Termos MeSH primário: Envelhecimento/metabolismo
Ácidos Docosa-Hexaenoicos/metabolismo
Ácido Eicosapentaenoico/metabolismo
Infarto do Miocárdio/metabolismo
[Mh] Termos MeSH secundário: Animais
Interleucina-1beta/metabolismo
Lipocalina-2/metabolismo
Masculino
Camundongos
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-1beta); 0 (Lipocalin-2); 0 (Tumor Necrosis Factor-alpha); 25167-62-8 (Docosahexaenoic Acids); AAN7QOV9EA (Eicosapentaenoic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.18632/aging.101077


  10 / 2025 MEDLINE  
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[PMID]:28954270
[Au] Autor:Wu J; Shao X; Lu K; Zhou J; Ren M; Xie X; Liu J; Xu Y; Ding Y; Shen X; Zhu C
[Ti] Título:Urinary RBP and NGAL Levels are Associated with Nephropathy in Patients with Type 2 Diabetes.
[So] Source:Cell Physiol Biochem;42(2):594-602, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: The diagnosis of type 2 diabetic nephropathy (T2DN) patients is important to prevent the long-term damaging effects of kidney loss in patients with diabetes and is decisive for patient outcomes. The aim of this study was to explore urine retinol binding protein (RBP) and neutrophil gelatinase-associated lipocalin (NGAL) in T2DN patients with and without albuminuria. METHODS: A total of 293 T2DN patients were divided into three groups according to their urine albumin/urine creatinine ratio (UACR): normoalbuminuria group (UACR<30 mg/g, n=100), microalbuminuria group (UACR 30-300 mg/g, n=100) and macroalbuminuria group (UACR>300 mg/g, n=93); 50 non-diabetic subjects were recruited as the control group. The levels of urine RBP, NGAL, TNF-α and IL-18 in T2DN patients and non-diabetic subjects were measured using ELISA assays. RESULTS: We first analyzed the clinical characteristics of the control and T2DN groups and found that urine NGAL, RBP, TNF-α and IL-18 levels were significantly increased and significantly correlated with the degree of albuminuria. In addition, univariate linear regression analysis showed that urine RBP was associated with UACR, BMI, Scr, BUN, TG, disease duration, SBP, NGAL, TNF-α and IL-18 levels, and urine NGAL was positively correlated with UACR, Scr, BUN, RBP, TNF-α and IL-18 levels. CONCLUSION: The results indicate that urine levels of NGAL and RBP may be independently associated with albuminuria in T2DN and may serve as novel biomarkers for the identification of T2DN.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/complicações
Diabetes Mellitus Tipo 2/urina
Nefropatias Diabéticas/complicações
Nefropatias Diabéticas/urina
Lipocalina-2/urina
Proteínas de Ligação ao Retinol/urina
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Albuminúria/complicações
Albuminúria/urina
Biomarcadores/urina
Nefropatias Diabéticas/diagnóstico
Feminino
Seres Humanos
Interleucina-18/urina
Masculino
Meia-Idade
Fator de Necrose Tumoral alfa/urina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Interleukin-18); 0 (LCN2 protein, human); 0 (Lipocalin-2); 0 (Retinol-Binding Proteins); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1159/000477860



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