Base de dados : MEDLINE
Pesquisa : D12.776.124.486 [Categoria DeCS]
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[PMID]:29355526
[Au] Autor:Bosc E; Nastri J; Lefort V; Valli M; Contiguiba F; Pioli R; Furlan M; Bolzani VDS; El Amri C; Reboud-Ravaux M
[Ad] Endereço:Sorbonne Université, UPMC Univ Paris 06-CNRS, IBPS, UMR 8256, Inserm ERL1164, B2A, 7 Quai Saint Bernard, F75005 Paris, France.
[Ti] Título:Piperlongumine and some of its analogs inhibit selectively the human immunoproteasome over the constitutive proteasome.
[So] Source:Biochem Biophys Res Commun;496(3):961-966, 2018 02 12.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The natural small molecule piperlongumine A is toxic selectively to cancer cells in vitro and in vivo. This toxicity has been correlated with cancer cell ROS, DNA damage and apoptotic cell death increases. We demonstrate here a new mechanistic property of piperlongumine: it inhibits selectively human immunoproteasome with no noticeable inhibition of human constitutive proteasome. This result suggests that immunoproteasome inhibition, a mechanism independent of ROS elevation, may also partly play a role in the anticancer effects observed with piperlongumine. Structure-activity relationships of piperlongumine analogs suggest that the lactam (piperidonic) ring of piperlongumine A may be replaced by the linear olefin -NHCO-CH =CH to improve both in vitro inhibitory efficiency against immunoproteasome and cellular toxicity.
[Mh] Termos MeSH primário: Apoptose/imunologia
Dioxolanos/química
Dioxolanos/imunologia
Imunoproteínas/química
Imunoproteínas/imunologia
Complexo de Endopeptidases do Proteassoma/química
Complexo de Endopeptidases do Proteassoma/imunologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Dioxolanos/administração & dosagem
Relação Dose-Resposta a Droga
Avaliação Pré-Clínica de Medicamentos
Células HeLa
Seres Humanos
Ligação Proteica
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dioxolanes); 0 (Immunoproteins); EC 3.4.25.1 (Proteasome Endopeptidase Complex); HN39MC8KIO (piperlonguminine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE


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[PMID]:28233060
[Au] Autor:Gaur R; Alam SI; Kamboj DV
[Ad] Endereço:Biotechnology Division, Defence Research and Development Establishment, Gwalior, 474002, India.
[Ti] Título:Immunoproteomic Analysis of Antibody Response of Rabbit Host Against Heat-Killed Francisella tularensis Live Vaccine Strain.
[So] Source:Curr Microbiol;74(4):499-507, 2017 Apr.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease Control and Prevention (CDC), vaccines and therapeutics are immediately required against this pathogen. In this study, an immunoproteomic approach based on the techniques of 2-dimensional gel electrophoresis (2DE) and immunoblotting combined with mass spectrometry (MS) was used for elucidation of immunogenic components and putative vaccine candidates. Whole-cell soluble protein extract of F. tularensis LVS (Ft LVS) was separated by 2DE, and immunoblots were developed with sera raised in rabbit after immunization with heat-killed Ft LVS. A total of 28 immunoreactive proteins were identified by tandem mass spectrometry. Rabbit immunoproteome of F. tularensis was compared with those previously reported using sera from human patients and in murine model. Out of 28 immunoreactive proteins identified in this study, 12 and 17 overlapping proteins were recognized by human and murine sera, respectively. Nine proteins were found immunogenic in all the three hosts, while eight new immunogenic proteins were found in this study. Identified immunoreactive proteins may find application in design and development of protein subunit vaccine for tularemia.
[Mh] Termos MeSH primário: Formação de Anticorpos/fisiologia
Vacinas Bacterianas/imunologia
Francisella tularensis/imunologia
Temperatura Alta
Imunoproteínas/análise
[Mh] Termos MeSH secundário: Animais
Eletroforese em Gel Bidimensional
Immunoblotting
Proteômica
Coelhos
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Vaccines); 0 (Immunoproteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-017-1217-y


  3 / 82 MEDLINE  
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[PMID]:27639588
[Au] Autor:Chen X; Wang Y; Fu M; Lei H; Cheng Q; Zhang X
[Ad] Endereço:Department of Neurology, Fujian Provincial Hospital, Fujian Medical University Provincial Clinical College, Fuzhou, China.
[Ti] Título:Plasma Immunoproteasome Predicts Early Hemorrhagic Transformation in Acute Ischemic Stroke Patients.
[So] Source:J Stroke Cerebrovasc Dis;26(1):49-56, 2017 Jan.
[Is] ISSN:1532-8511
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND PURPOSE: Currently, blood biomarkers associated with an increased hemorrhagic transformation (HT) risk remain uncertain. We aimed to determine the significance of immunoproteasome as predictors of early HT in acute ischemic stroke patients. METHODS: This study enrolled 316 patients with ischemic stroke. HT was assessed by computed tomography examination performed on day 5 ± 2 after stroke onset or immediately in case of clinical deterioration (CD). Plasma immunoproteasome subunits low molecular mass peptide 2 (LMP2), multicatalytic endopeptidase complex-like 1 (MECL-1), LMP7, interleukin-1ß (IL1ß), and high-sensitivity C-reactive protein (Hs-CRP) were measured with quantitative sandwich enzyme-linked immunosorbent assay kits. Factors associated with HT were analyzed using a multivariate logistic regression analysis. RESULTS: There were 42 (13.3%, 42 of 316) patients who experienced HT. Compared with those patients without HT, plasma LMP2, MECL-1, LMP7, IL1ß, and Hs-CRP concentrations on admission were significantly increased in patients with subsequent HT (P < .001). These protein concentrations increased with hemorrhage severity. Patients with CD caused by HT had the highest levels of LMP2 (1679.5 [1394.6-136.6] pg/mL), MECL-1 (992.5 [849.7-1075.8] pg/mL), LMP7 (822.6 [748.6-1009.5] pg/mL), IL1ß (113.2 [90.6-194.5] pg/mL), and Hs-CRP (30.0 [12.8-75.6] mg/L) (P < .05). Logistic regression analysis showed cardioembolism, LMP2, MECL-1, and LMP7 as independent predictors of HT (P < .05). Receiver operating characteristic curve analysis demonstrated LMP2 ≥ 988.3 pg/mL, MECL-1 ≥ 584.7 pg/mL, and LMP7 ≥ 509.0 pg/mL as independent factors associated with HT (P < .001). CONCLUSION: Evaluation of plasma levels of immunoproteasome could be helpful in the early prediction of HT in acute ischemic stroke patients.
[Mh] Termos MeSH primário: Imunoproteínas/metabolismo
Hemorragias Intracranianas/sangue
Hemorragias Intracranianas/diagnóstico
Hemorragias Intracranianas/etiologia
Complexo de Endopeptidases do Proteassoma/sangue
Acidente Vascular Cerebral/complicações
[Mh] Termos MeSH secundário: Idoso
Isquemia Encefálica/complicações
Proteína C-Reativa/metabolismo
Cisteína Endopeptidases/sangue
Citocinas/sangue
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Modelos Logísticos
Masculino
Meia-Idade
Acidente Vascular Cerebral/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Immunoproteins); 144416-78-4 (LMP-2 protein); 9007-41-4 (C-Reactive Protein); EC 3.4.22.- (Cysteine Endopeptidases); EC 3.4.25.1 (Proteasome Endopeptidase Complex)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160919
[St] Status:MEDLINE


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[PMID]:27506451
[Au] Autor:Farini A; Sitzia C; Cassani B; Cassinelli L; Rigoni R; Colleoni F; Fusco N; Gatti S; Bella P; Villa C; Napolitano F; Maiavacca R; Bosari S; Villa A; Torrente Y
[Ad] Endereço:Stem Cell Laboratory, Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Unit of Neurology, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Milan, Italy.
[Ti] Título:Therapeutic Potential of Immunoproteasome Inhibition in Duchenne Muscular Dystrophy.
[So] Source:Mol Ther;24(11):1898-1912, 2016 Nov.
[Is] ISSN:1525-0024
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Duchenne muscular dystrophy is an inherited fatal genetic disease characterized by mutations in dystrophin gene, causing membrane fragility leading to myofiber necrosis and inflammatory cell recruitment in dystrophic muscles. The resulting environment enriched in proinflammatory cytokines, like IFN-γ and TNF-α, determines the transformation of myofiber constitutive proteasome into the immunoproteasome, a multisubunit complex involved in the activation of cell-mediate immunity. This event has a fundamental role in producing peptides for antigen presentation by MHC class I, for the immune response and also for cytokine production and T-cell differentiation. Here, we characterized for the first time the presence of T-lymphocytes activated against revertant dystrophin epitopes, in the animal model of Duchenne muscular dystrophy, the mdx mice. Moreover, we specifically blocked i-proteasome subunit LMP7, which was up-regulated in dystrophic skeletal muscles, and we demonstrated the rescue of the dystrophin expression and the amelioration of the dystrophic phenotype. The i-proteasome blocking lowered myofiber MHC class I expression and self-antigen presentation to T cells, thus reducing the specific antidystrophin T cell response, the muscular cell infiltrate, and proinflammatory cytokine production, together with muscle force recovery. We suggest that i-proteasome inhibition should be considered as new promising therapeutic approach for Duchenne muscular dystrophy pathology.
[Mh] Termos MeSH primário: Imunoproteínas/antagonistas & inibidores
Distrofia Muscular de Duchenne/tratamento farmacológico
Inibidores de Proteassoma/administração & dosagem
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Modelos Animais de Doenças
Terapia Genética
Camundongos
Camundongos Endogâmicos mdx
Distrofia Muscular de Duchenne/imunologia
Oligopeptídeos/administração & dosagem
Oligopeptídeos/farmacologia
Complexo de Endopeptidases do Proteassoma/metabolismo
Inibidores de Proteassoma/farmacologia
Linfócitos T/efeitos dos fármacos
Linfócitos T/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoproteins); 0 (Oligopeptides); 0 (PR-957); 0 (Proteasome Inhibitors); EC 3.4.25.1 (LMP7 protein); EC 3.4.25.1 (Proteasome Endopeptidase Complex)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160811
[St] Status:MEDLINE
[do] DOI:10.1038/mt.2016.162


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[PMID]:27390057
[Au] Autor:Silva N; Patrício E; Bettencourt P; Guimarães JT
[Ad] Endereço:Unidade I&D Cardiovascular do Porto, Faculdade de Medicina da Universidade do Porto, Porto, Portugal. nunosilva.box@gmail.com.
[Ti] Título:Evaluation of Innate Immunity Biomarkers on Admission and at Discharge From an Acute Heart Failure Episode.
[So] Source:J Clin Lab Anal;30(6):1183-1190, 2016 Nov.
[Is] ISSN:1098-2825
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The involvement of the immune system in heart failure (HF) has been demonstrated. Evidence shows that innate immunity can have a role in the remodeling process and progression of HF. With previous studies showing the prognostic value of some innate immunity markers and their relevance in this condition, we aim to evaluate how these markers vary on hospitalization due to an acute episode of HF and at discharge. METHODS: About 154 patients admitted with acute HF were prospectively recruited. Patients were evaluated on admission and at discharge from the hospital. Patients with infection were separately analyzed. Innate immunity, inflammatory, and cardiac biomarkers were measured and were compared between groups and between admission and discharge and with reference values of biological variation. RESULTS: Median patients' age was 78 years, and half of the patients were men. The median duration of hospitalization was 6 days. C3 and C4 protein levels significantly increased (P < 0.001) between admission and discharge, as well as eosinophils (P < 0.001) and BNP levels decreased (P < 0.001). Variation in all these variables was independent of infection and biological variation. CONCLUSION: Our results show that innate immunity markers such as C3 and C4 increase after treatment for acute HF, supporting the hypothesis that they can be involved in the resolution of the acute episode.
[Mh] Termos MeSH primário: Eosinófilos/patologia
Insuficiência Cardíaca
Hospitalização
Sistema Imunitário/fisiopatologia
Imunoproteínas/metabolismo
[Mh] Termos MeSH secundário: Doença Aguda
Idoso
Idoso de 80 Anos ou mais
Biomarcadores/metabolismo
Complemento C3/metabolismo
Complemento C4/metabolismo
Ecocardiografia
Feminino
Insuficiência Cardíaca/imunologia
Insuficiência Cardíaca/metabolismo
Insuficiência Cardíaca/terapia
Seres Humanos
Imunidade Inata/fisiologia
Masculino
Peptídeo Natriurético Encefálico/metabolismo
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Biomarkers); 0 (Complement C3); 0 (Complement C4); 0 (Immunoproteins); 114471-18-0 (Natriuretic Peptide, Brain)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE
[do] DOI:10.1002/jcla.22001


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[PMID]:27198787
[Au] Autor:Lima BS; Fialho LC; Pires SF; Tafuri WL; Andrade HM
[Ad] Endereço:Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, 31279-910 Belo Horizonte, Minas Gerais, Brazil. Electronic address: brunasoaressl@yahoo.com.br.
[Ti] Título:Immunoproteomic and bioinformatic approaches to identify secreted Leishmania amazonensis, L. braziliensis, and L. infantum proteins with specific reactivity using canine serum.
[So] Source:Vet Parasitol;223:115-9, 2016 Jun 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Leishmania spp have a wide range of hosts, and each host can harbor several Leishmania species. Dogs, for example, are frequently infected by Leishmania infantum, where they constitute its main reservoir, but they also serve as hosts for L. braziliensis and L. amazonensis. Serological tests for antibody detection are valuable tools for diagnosis of L. infantum infection due to the high levels of antibodies induced, unlike what is observed in L. amazonensis and L. braziliensis infections. Likewise, serology-based antigen-detection can be useful as an approach to diagnose any Leishmania species infection using different corporal fluid samples. Immunogenic and secreted proteins constitute powerful targets for diagnostic methods in antigen detection. As such, we performed immunoproteomic (2-DE, western blot and mass spectrometry) and bioinformatic screening to search for reactive and secreted proteins from L. amazonensis, L. braziliensis, and L. infantum. Twenty-eight non-redundant proteins were identified, among which, six were reactive only in L. amazonensis extracts, 10 in L. braziliensis extracts, and seven in L. infantum extracts. After bioinformatic analysis, seven proteins were predicted to be secreted, two of which were reactive only in L. amazonensis extracts (52kDa PDI and the glucose-regulated protein 78), one in L. braziliensis extracts (pyruvate dehydrogenase E1 beta subunit) and three in L. infantum extracts (two conserved hypothetical proteins and elongation factor 1-beta). We propose that proteins can be suitable targets for diagnostic methods based on antigen detection.
[Mh] Termos MeSH primário: Biologia Computacional/métodos
Doenças do Cão/parasitologia
Imunoproteínas/isolamento & purificação
Leishmania/classificação
Leishmaniose/veterinária
Proteômica/métodos
[Mh] Termos MeSH secundário: Animais
Doenças do Cão/diagnóstico
Cães
Leishmania/isolamento & purificação
Leishmaniose/diagnóstico
Leishmaniose/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoproteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160521
[St] Status:MEDLINE


  7 / 82 MEDLINE  
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[PMID]:27160601
[Au] Autor:Dierking K; Yang W; Schulenburg H
[Ad] Endereço:Department of Evolutionary Ecology and Genetics, University of Kiel, Kiel 24098, Germany.
[Ti] Título:Antimicrobial effectors in the nematode Caenorhabditis elegans: an outgroup to the Arthropoda.
[So] Source:Philos Trans R Soc Lond B Biol Sci;371(1695), 2016 05 26.
[Is] ISSN:1471-2970
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nematodes and arthropods likely form the taxon Ecdysozoa. Information on antimicrobial effectors from the model nematode Caenorhabditis elegans may thus shed light on the evolutionary origin of these defences in arthropods. This nematode species possesses an extensive armory of putative antimicrobial effector proteins, such as lysozymes, caenopores (or saposin-like proteins), defensin-like peptides, caenacins and neuropeptide-like proteins, in addition to the production of reactive oxygen species and autophagy. As C. elegans is a bacterivore that lives in microbe-rich environments, some of its effector peptides and proteins likely function in both digestion of bacterial food and pathogen elimination. In this review, we provide an overview of C. elegans immune effector proteins and mechanisms. We summarize the experimental evidence of their antimicrobial function and involvement in the response to pathogen infection. We further evaluate the microbe-induced expression of effector genes using WormExp, a recently established database for C. elegans gene expression analysis. We emphasize the need for further analysis at the protein level to demonstrate an antimicrobial activity of these molecules both in vitro and in vivoThis article is part of the themed issue 'Evolutionary ecology of arthropod antimicrobial peptides'.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/genética
Proteínas de Caenorhabditis elegans/genética
Caenorhabditis elegans/genética
Caenorhabditis elegans/microbiologia
Interações Hospedeiro-Patógeno
[Mh] Termos MeSH secundário: Animais
Peptídeos Catiônicos Antimicrobianos/metabolismo
Caenorhabditis elegans/virologia
Proteínas de Caenorhabditis elegans/metabolismo
Imunoproteínas/genética
Imunoproteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Caenorhabditis elegans Proteins); 0 (Immunoproteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE


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[PMID]:27160598
[Au] Autor:Makarova O; Rodríguez-Rojas A; Eravci M; Weise C; Dobson A; Johnston P; Rolff J
[Ad] Endereço:Institut für Biologie, Evolutionary Biology, Freie Universität Berlin, Königin-Luise-Straße 1-3, Berlin 14195, Germany.
[Ti] Título:Antimicrobial defence and persistent infection in insects revisited.
[So] Source:Philos Trans R Soc Lond B Biol Sci;371(1695), 2016 05 26.
[Is] ISSN:1471-2970
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Insects show long-lasting antimicrobial immune responses that follow the initial fast-acting cellular processes. These immune responses are discussed to provide a form of phrophylaxis and/or to serve as a safety measure against persisting infections. The duration and components of such long-lasting responses have rarely been studied in detail, a necessary prerequisite to understand their adaptive value. Here, we present a 21 day proteomic time course of the mealworm beetle Tenebrio molitor immune-challenged with heat-killed Staphylococcus aureus The most upregulated peptides are antimicrobial peptides (AMPs), many of which are still highly abundant 21 days after infection. The identified AMPs included toll and imd-mediated AMPs, a significant number of which have no known function against S. aureus or other Gram-positive bacteria. The proteome reflects the selective arena for bacterial infections. The results also corroborate the notion of synergistic interactions in vivo that are difficult to model in vitroThis article is part of the themed issue 'Evolutionary ecology of arthropod antimicrobial peptides'.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/genética
Imunidade Inata
Proteínas de Insetos/genética
Staphylococcus aureus/fisiologia
Tenebrio/imunologia
Tenebrio/microbiologia
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/metabolismo
Peptídeos Catiônicos Antimicrobianos/metabolismo
Imunoproteínas/genética
Imunoproteínas/metabolismo
Proteínas de Insetos/metabolismo
Proteômica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antimicrobial Cationic Peptides); 0 (Immunoproteins); 0 (Insect Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE


  9 / 82 MEDLINE  
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PubMed Central Texto completo
Texto completo
[PMID]:27089967
[Au] Autor:Yi L; Wang Y; Ma Z; Lin HX; Xu B; Grenier D; Fan HJ; Lu CP
[Ad] Endereço:Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
[Ti] Título:Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation.
[So] Source:Vet Res;47:50, 2016 Apr 18.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Streptococcus equi ssp. zooepidemicus (S. equi spp. zooepidemicus) is an opportunistic pathogen that causes major economic losses in the swine industry in China and is also a threat for human health. Biofilm formation by this bacterium has been previously reported. In this study, we used an immunoproteomic approach to search for immunogenic proteins expressed by biofilm-grown S. equi spp. zooepidemicus. Seventeen immunoreactive proteins were found, of which nine common immunoreactive proteins were identified in planktonic and biofilm-grown bacteria. The immunogenicity and protective efficacy of the S. equi spp. zooepidemicus immunoreactive GroEL chaperone protein was further investigated in mice. The protein was expressed in vivo and elicited high antibody titers following S. equi spp. zooepidemicus infections of mice. An animal challenge experiment with S. equi spp. zooepidemicus showed that 75% of mice immunized with the GroEL protein were protected. Using in vitro biofilm inhibition assays, evidence was obtained that the chaperonin GroEL may represent a promising target for the prevention and treatment of persistent S. equi spp. zooepidemicus biofilm infections. In summary, our results suggest that the recombinant GroEL protein, which is involved in biofilm formation, may efficiently stimulate an immune response, which protects against S. equi spp. zooepidemicus infections. It may therefore be a candidate of interest to be included in vaccines against S. equi spp. zooepidemicus infections.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Biofilmes
Chaperonina 60/genética
Streptococcus equi/fisiologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos
Proteínas de Bactérias/imunologia
Chaperonina 60/imunologia
Feminino
Imunização
Imunoproteínas/genética
Imunoproteínas/imunologia
Camundongos
Camundongos Endogâmicos ICR
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Streptococcus equi/genética
Streptococcus equi/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Bacterial Proteins); 0 (Chaperonin 60); 0 (Immunoproteins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160420
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-016-0334-0


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[PMID]:26756824
[Au] Autor:Kammerl IE; Dann A; Mossina A; Brech D; Lukas C; Vosyka O; Nathan P; Conlon TM; Wagner DE; Overkleeft HS; Prasse A; Rosas IO; Straub T; Krauss-Etschmann S; Königshoff M; Preissler G; Winter H; Lindner M; Hatz R; Behr J; Heinzelmann K; Yildirim AÖ; Noessner E; Eickelberg O; Meiners S
[Ad] Endereço:1 Comprehensive Pneumology Center, University Hospital, Ludwig-Maximilians University, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
[Ti] Título:Impairment of Immunoproteasome Function by Cigarette Smoke and in Chronic Obstructive Pulmonary Disease.
[So] Source:Am J Respir Crit Care Med;193(11):1230-41, 2016 Jun 01.
[Is] ISSN:1535-4970
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Patients with chronic obstructive pulmonary disease (COPD) and in particular smokers are more susceptible to respiratory infections contributing to acute exacerbations of disease. The immunoproteasome is a specialized type of proteasome destined to improve major histocompatibility complex (MHC) class I-mediated antigen presentation for the resolution of intracellular infections. OBJECTIVES: To characterize immunoproteasome function in COPD and its regulation by cigarette smoke. METHODS: Immunoproteasome expression and activity were determined in bronchoalveolar lavage (BAL) and lungs of human donors and patients with COPD or idiopathic pulmonary fibrosis (IPF), as well as in cigarette smoke-exposed mice. Smoke-mediated alterations of immunoproteasome activity and MHC I surface expression were analyzed in human blood-derived macrophages. Immunoproteasome-specific MHC I antigen presentation was evaluated in spleen and lung immune cells that had been smoke-exposed in vitro or in vivo. MEASUREMENTS AND MAIN RESULTS: Immunoproteasome and MHC I mRNA expression was reduced in BAL cells of patients with COPD and in isolated alveolar macrophages of patients with COPD or IPF. Exposure of immune cells to cigarette smoke extract in vitro reduced immunoproteasome activity and impaired immunoproteasome-specific MHC I antigen presentation. In vivo, acute cigarette smoke exposure dynamically regulated immunoproteasome function and MHC I antigen presentation in mouse BAL cells. End-stage COPD lungs showed markedly impaired immunoproteasome activities. CONCLUSIONS: We here show that the activity of the immunoproteasome is impaired by cigarette smoke resulting in reduced MHC I antigen presentation. Regulation of immunoproteasome function by cigarette smoke may thus alter adaptive immune responses and add to prolonged infections and exacerbations in COPD and IPF.
[Mh] Termos MeSH primário: Imunoproteínas/fisiologia
Doença Pulmonar Obstrutiva Crônica/fisiopatologia
Fumaça/efeitos adversos
Fumar/fisiopatologia
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Animais
Modelos Animais de Doenças
Feminino
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Meia-Idade
Tabaco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoproteins); 0 (Smoke)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160113
[St] Status:MEDLINE
[do] DOI:10.1164/rccm.201506-1122OC



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