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[PMID]:27932254
[Au] Autor:Lodovicho ME; Costa TR; Bernardes CP; Menaldo DL; Zoccal KF; Carone SE; Rosa JC; Pucca MB; Cerni FA; Arantes EC; Tytgat J; Faccioli LH; Pereira-Crott LS; Sampaio SV
[Ad] Endereço:Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
[Ti] Título:Investigating possible biological targets of Bj-CRP, the first cysteine-rich secretory protein (CRISP) isolated from Bothrops jararaca snake venom.
[So] Source:Toxicol Lett;265:156-169, 2017 Jan 04.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cysteine-rich secretory proteins (CRISPs) are commonly described as part of the protein content of snake venoms, nevertheless, so far, little is known about their biological targets and functions. Our study describes the isolation and characterization of Bj-CRP, the first CRISP isolated from Bothrops jararaca snake venom, also aiming at the identification of possible targets for its actions. Bj-CRP was purified using three chromatographic steps (Sephacryl S-200, Source 15Q and C18) and showed to be an acidic protein of 24.6kDa with high sequence identity to other snake venom CRISPs. This CRISP was devoid of proteolytic, hemorrhagic or coagulant activities, and it did not affect the currents from 13 voltage-gated potassium channel isoforms. Conversely, Bj-CRP induced inflammatory responses characterized by increase of leukocytes, mainly neutrophils, after 1 and 4h of its injection in the peritoneal cavity of mice, also stimulating the production of IL-6. Bj-CRP also acted on the human complement system, modulating some of the activation pathways and acting directly on important components (C3 and C4), thus inducing the generation of anaphylatoxins (C3a, C4a and C5a). Therefore, our results for Bj-CRP open up prospects for better understanding this class of toxins and its biological actions.
[Mh] Termos MeSH primário: Bothrops
Venenos de Crotalídeos/química
Peptídeos/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Anafilatoxinas/biossíntese
Anafilatoxinas/imunologia
Animais
Coagulação Sanguínea/efeitos dos fármacos
Células Cultivadas
Ativação do Complemento/efeitos dos fármacos
Eletroforese em Gel de Poliacrilamida
Hemorragia/induzido quimicamente
Seres Humanos
Técnicas In Vitro
Masculino
Camundongos Endogâmicos C57BL
Peso Molecular
Oócitos/efeitos dos fármacos
Oócitos/metabolismo
Técnicas de Patch-Clamp
Peptídeos/farmacologia
Peptídeos/toxicidade
Canais de Potássio de Abertura Dependente da Tensão da Membrana/antagonistas & inibidores
Proteínas de Répteis/isolamento & purificação
Proteínas de Répteis/farmacologia
Proteínas de Répteis/toxicidade
Venenos de Víboras/isolamento & purificação
Venenos de Víboras/farmacologia
Venenos de Víboras/toxicidade
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anaphylatoxins); 0 (Bj-CRP protein, Bothrops jararaca); 0 (Crotalid Venoms); 0 (Peptides); 0 (Potassium Channels, Voltage-Gated); 0 (Reptilian Proteins); 0 (Viper Venoms)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE


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[PMID]:27846429
[Au] Autor:Menaldo DL; Bernardes CP; Jacob-Ferreira AL; Nogueira-Santos CG; Casare-Ogasawara TM; Pereira-Crott LS; Sampaio SV
[Ad] Endereço:Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: danmenaldo@yahoo.com.br.
[Ti] Título:Effects of Bothrops atrox venom and two isolated toxins on the human complement system: Modulation of pathways and generation of anaphylatoxins.
[So] Source:Mol Immunol;80:91-100, 2016 Dec.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The complement system plays important biological roles, including the activation of inflammatory processes in response to the generation of proteolytic fragments of its components. Here we evaluated the effects of Bothrops atrox venom and two of its toxins (the P-I metalloprotease Batroxase and the acidic phospholipase A BatroxPLA ) on the human complement system, evaluating their effects on the classical (CP), lectin (LP) and alternative (AP) pathways, as well as on different complement components associated to the generation of anaphylatoxins. Primarily, the venom and both toxins modulated the hemolytic activity of the complement CP, with the venom and Batroxase reducing this activity and BatroxPLA increasing it. ELISA deposition assays indicated that B. atrox venom and Batroxase were also capable of modulating all three activation pathways (CP, LP and AP), reducing their activity after incubation with normal human serum (NHS), while BatroxPLA apparently only interfered with AP. Additionally, the venom and Batroxase, but not BatroxPLA , promoted significant degradation of the components C3, C4, Factor B and C1-Inhibitor, as shown by Western blot and SDS-PAGE analyses, also generating anaphylatoxins C3a, C4a and C5a. Therefore, B. atrox venom and Batroxase were able to activate the complement system by direct proteolytic action on several components, generating anaphylatoxins and affecting the activation pathways, while BatroxPLA only interfered with the hemolysis induced by CP and the C3 deposition related to AP. Our results indicate that Batroxase and possibly other metalloproteases should be the main toxins in B. atrox venom to induce pronounced effects on the complement system.
[Mh] Termos MeSH primário: Anafilatoxinas/metabolismo
Bothrops
Ativação do Complemento/efeitos dos fármacos
Proteínas do Sistema Complemento/imunologia
Venenos de Crotalídeos/toxicidade
[Mh] Termos MeSH secundário: Animais
Western Blotting
Ensaio de Imunoadsorção Enzimática
Seres Humanos
Imunoensaio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anaphylatoxins); 0 (Crotalid Venoms); 9007-36-7 (Complement System Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE


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[PMID]:27280846
[Au] Autor:Javaux C; Stordeur P; Azarkan M; Mascart F; Baeyens-Volant D
[Ad] Endereço:Protein Chemistry Unit, Faculté de Médecine, Université Libre de Bruxelles (ULB), Brussels, Belgium. Electronic address: cjavaux@ulb.ac.be.
[Ti] Título:Isolation of a thiol-dependent serine protease in peanut and investigation of its role in the complement and the allergic reaction.
[So] Source:Mol Immunol;75:133-43, 2016 07.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A serine protease activity was detected in aqueous peanuts seeds extracts, partially purified and characterized as a thiol-dependent serine protease. The potential role of this proteolytic activity on allergic reaction to peanuts was prospected through complement activation studies in human plasma and serum, and MDCK cells to investigate a possible occludin degradation in tight junctions. The peanut protease activity induced the production of anaphylatoxins C3a and C5a, and of the terminal membrane attack complex SC5b-9 whatever the complement activation pathway. The protease activity was also involved in the partial digestion of occludin within tight junctions, with for result, an increase of the epithelial permeability to antigen absorption.
[Mh] Termos MeSH primário: Anafilatoxinas/imunologia
Arachis/enzimologia
Hipersensibilidade a Amendoim/enzimologia
Serina Proteases/imunologia
[Mh] Termos MeSH secundário: Anafilatoxinas/química
Animais
Arachis/química
Cromatografia de Afinidade
Cães
Seres Humanos
Células Madin Darby de Rim Canino
Serina Proteases/química
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Compostos de Sulfidrila
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anaphylatoxins); 0 (Sulfhydryl Compounds); EC 3.4.- (Serine Proteases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160610
[St] Status:MEDLINE


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[PMID]:26934108
[Au] Autor:Li MF; Hu YH
[Ad] Endereço:Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
[Ti] Título:C5a of Cynoglossus semilaevis has anaphylatoxin-like properties and promotes antibacterial and antiviral defense.
[So] Source:Dev Comp Immunol;60:139-48, 2016 Jul.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Activation of the complement system leads to the cleavage of component factor C5 into C5a and C5b. C5a can induce chemotaxis and inflammatory responses in mammals. The function of C5a in fish is poorly understood. In this study, we report the identification and analysis of a C5 homologue, CsC5, from tongue sole (Cynoglossus semilaevis). CsC5 is composed of 1683 amino acid residues that include an anaphylatoxin homologous domain. Expression of CsC5 could be detected in a variety of tissues and was up-regulated by bacterial or viral pathogen infection. Purified recombinant CsC5a (rCsC5a) could bind to peripheral blood leukocytes (PBL) and stimulate PBL chemotaxis, proliferation, respiratory burst, acid phosphatase activity, and phagocytosis. Tongue sole administered rCsC5a exhibited enhanced resistance against bacterial and viral infections. These results indicate that CsC5a is an anaphylatoxin with a role in innate immune defense against bacterial and viral infections.
[Mh] Termos MeSH primário: Complemento C5a/fisiologia
Doenças dos Peixes/imunologia
Proteínas de Peixes/fisiologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/farmacologia
Sequência de Aminoácidos
Anafilatoxinas/farmacologia
Animais
Células Cultivadas
Quimiotaxia
Complemento C5a/farmacologia
Sequência Conservada
Escherichia coli/imunologia
Doenças dos Peixes/metabolismo
Doenças dos Peixes/microbiologia
Proteínas de Peixes/farmacologia
Linguados
Imunidade Inata/efeitos dos fármacos
Rim/imunologia
Rim/metabolismo
Rim/microbiologia
Leucócitos Mononucleares/efeitos dos fármacos
Leucócitos Mononucleares/imunologia
Leucócitos Mononucleares/microbiologia
Especificidade de Órgãos
Fagocitose/efeitos dos fármacos
Baço/imunologia
Baço/metabolismo
Baço/microbiologia
Vibrio/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Anaphylatoxins); 0 (Fish Proteins); 80295-54-1 (Complement C5a)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160303
[St] Status:MEDLINE


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[PMID]:26616865
[Au] Autor:Horellou P; Wang M; Keo V; Chrétien P; Serguera C; Waters P; Deiva K
[Ad] Endereço:Inserm, U 1184, Center for Immunology of Viral Infections and Autoimmune Diseases, Université Paris-Sud 11, CEA, DSV/iMETI, Division of Immuno-Virology, IDMIT, Faculté de médecine - 63, rue Gabriel Péri, 94276 Le Kremlin-Bicêtre Cedex, France. Electronic address: philippe.horellou@inserm.fr.
[Ti] Título:Increased interleukin-6 correlates with myelin oligodendrocyte glycoprotein antibodies in pediatric monophasic demyelinating diseases and multiple sclerosis.
[So] Source:J Neuroimmunol;289:1-7, 2015 Dec 15.
[Is] ISSN:1872-8421
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Acquired demyelinating syndromes (ADS) in children evolve either as a monophasic disease diagnosed as acute demyelinating encephalomyelitis (ADEM), transverse myelitis (TM) or optic neuritis (ON), or a multiphasic one with several relapses most often leading to the diagnosis of multiple sclerosis (MS) or neuromyelitis optica (NMO). These neuroinflammatory disorders are increasingly associated with autoantibodies against proteins such as aquaporin-4 in rare instances, and more frequently against myelin oligodendrocyte glycoprotein (MOG). Recently, in adult NMO patients, C5a levels were shown to be elevated in cerebrospinal fluid (CSF) during acute exacerbation. We investigated the CSF levels of anaphylatoxins and pro-inflammatory cytokines, and plasma MOG antibodies in onset samples from children with ADS. Thirty four children presenting with a first episode of ADS, 17 with monophasic ADS (9 with ADEM, 4 with TM and 4 with ON) and 17 with MS, who had paired blood and CSF samples at onset were included and compared to 12 patients with other non-inflammatory neurological disorders (OND). Cytokines and anaphylatoxins in CSF were measured by Cytometric Bead Array immunoassay. MOG antibody titers in plasma were tested by flow cytometry using a stable cell line expressing full-length human MOG. We found a significant increase in C5a levels in the CSF of patients with monophasic ADS (n=17) compared to OND (n=12, p=0.0036) and to MS (n=17, p=0.0371). The C5a levels in MS were higher than in OND without reaching significance (p=0.2). CSF IL-6 levels were significantly increased in monophasic ADS compared to OND (p=0.0027) and to MS (p=0.0046). MOG antibody plasma levels were significantly higher in monophasic ADS (p<0.0001) and, to a lesser extent, in MS compared to OND (p=0.0023). Plasma MOG antibodies and CSF IL-6 levels were significantly correlated (r=0.51, p=0.018). CSF C5a and IL-6 levels are increased in monophasic ADS but not in MS when compared to OND, suggesting that these markers may help to predict monophasic or relapsing fate of ADS at onset. MOG antibody titers, which were higher in monophasic ADS than in MS, correlated with IL-6 levels, but not with C5a, suggesting an association between MOG antibodies and neuroinflammation in pediatric ADS.
[Mh] Termos MeSH primário: Doenças Desmielinizantes
Imunoglobulina G/sangue
Interleucina-6/imunologia
Esclerose Múltipla
Glicoproteína Mielina-Oligodendrócito/imunologia
[Mh] Termos MeSH secundário: Adolescente
Anafilatoxinas/líquido cefalorraquidiano
Anafilatoxinas/imunologia
Anafilatoxinas/metabolismo
Criança
Citocinas/metabolismo
Doenças Desmielinizantes/sangue
Doenças Desmielinizantes/líquido cefalorraquidiano
Doenças Desmielinizantes/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Interleucina-6/sangue
Interleucina-6/líquido cefalorraquidiano
Estudos Longitudinais
Masculino
Esclerose Múltipla/sangue
Esclerose Múltipla/líquido cefalorraquidiano
Esclerose Múltipla/imunologia
Glicoproteína Mielina-Oligodendrócito/sangue
Glicoproteína Mielina-Oligodendrócito/líquido cefalorraquidiano
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anaphylatoxins); 0 (Cytokines); 0 (Immunoglobulin G); 0 (Interleukin-6); 0 (Myelin-Oligodendrocyte Glycoprotein)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:151130
[Lr] Data última revisão:
151130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151201
[St] Status:MEDLINE


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[PMID]:26289385
[Au] Autor:Khan MA; Assiri AM; Broering DC
[Ad] Endereço:Department of Comparative Medicine, King Faisal Specialist Hospital & Research Centre, P.O. Box 3354, Riyadh, 11211 MBC-03, Kingdom of Saudi Arabia. mkhan26@kfshrc.edu.sa.
[Ti] Título:Complement mediators: key regulators of airway tissue remodeling in asthma.
[So] Source:J Transl Med;13:272, 2015 Aug 20.
[Is] ISSN:1479-5876
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The complement mediators are the major effectors of the immune balance, which operates at the interface between the innate and adaptive immunity, and is vital for many immunoregulatory functions. Activation of the complement cascade through the classical, alternative or lectin pathways thus generating opsonins like C3b and C5b, anaphylatoxins C3a and C5a, chemotaxin, and inflammatory mediators, which leads to cellular death. Complement mediators that accelerate the airway remodeling are not well defined; however, an uncontrolled Th2-driven adaptive immune response has been linked to the major pathophysiologic features of asthma, including bronchoconstriction, airway hyperresponsiveness, and airway inflammation. The mechanisms leading to complement mediated airway tissue remodeling, and the effect of therapy on preventing and/or reversing it are not clearly understood. This review highlights complement-mediated inflammation, and the mechanism through it triggers the airway tissue injury and remodeling in the airway epithelium that could serve as potential targets for developing a new drug to rescue the asthma patients.
[Mh] Termos MeSH primário: Remodelação das Vias Aéreas
Asma/imunologia
Ativação do Complemento
Complemento C3a/imunologia
Complemento C5a/imunologia
[Mh] Termos MeSH secundário: Anafilatoxinas/imunologia
Animais
Asma/patologia
Fatores Quimiotáticos/imunologia
Seres Humanos
Imunidade Inata
Inflamação/imunologia
Mediadores da Inflamação/imunologia
Interleucina-13/imunologia
Proteínas Opsonizantes/imunologia
Células Th2/citologia
Fator de Crescimento Transformador beta/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anaphylatoxins); 0 (Chemotactic Factors); 0 (Inflammation Mediators); 0 (Interleukin-13); 0 (Opsonin Proteins); 0 (Transforming Growth Factor beta); 80295-42-7 (Complement C3a); 80295-54-1 (Complement C5a)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150821
[St] Status:MEDLINE
[do] DOI:10.1186/s12967-015-0565-2


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[PMID]:26256795
[Au] Autor:Kumar PS; Mauriello CT; Hair PS; Rister NS; Lawrence C; Raafat RH; Cunnion KM
[Ad] Endereço:855 W. Brambleton Avenue, Eastern Virginia Medical School, Norfolk, VA 23507, USA. Electronic address: Parvathi.kumar@chkd.org.
[Ti] Título:Glucose-based dialysis fluids inhibit innate defense against Staphylococcus aureus.
[So] Source:Mol Immunol;67(2 Pt B):575-83, 2015 Oct.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Staphylococcus aureus peritonitis is a serious complication of Chronic Peritoneal Dialysis (CPD) and associated with a higher risk for severe and recurrent infections compared with other bacteria. We have previously shown that complement-mediated effectors essential for optimal opsonophagocytosis of S. aureus are inhibited by high glucose concentrations. Since most commonly used peritoneal dialysis (PD) fluids are glucose-based, we hypothesized that glucose-based PD fluids likely inhibit complement host defenses against S. aureus. METHODS: Commercially available PD fluids were tested: glucose-based (Dianeal), Dianeal supplemented with amino acids, icodextrin-based (Extraneal) and amino acid-based (Nutrineal). Control PD fluid was generated to simulate Dianeal excluding the glucose. Three commercially available glucose concentrations were tested: Dianeal 1.5% (15 gm/1000 ml), Dianeal 2.5% (25 gm/1000 ml) and Dianeal 4.25% (42.5 gm/1000 ml). Complement effectors against S. aureus were analyzed including opsonization with C3-fragments, anaphylatoxin generation, and phagocytosis efficiency. We also evaluated clinical strains, including MRSA strains, and specific complement activation pathways. RESULTS: Glucose-based PD fluids inhibited complement opsonization of S. aureus (≥7-fold reduction) and inhibited S. aureus-induced generation of anaphylatoxins C3a and C5a (>10-fold reduction) compared to non-glucose based PD fluids. Dianeal 1.5%, 2.5% and 4.25%, all similarly inhibited C3-mediated opsonization. Glucose-based PD fluids showed a ≥4-fold reduction in opsonization of clinical strains of S.aureus, including MRSA strains. Decreased opsonization of S.aureus in the glucose-based PD fluid compared with non-glucose based fluids correlated with decreased phagocytosis by neutrophils. CONCLUSION: Complement-mediated opsonophagocytosis of S. aureus and anaphylatoxin generation were severely inhibited in glucose-based PD fluids compared with non-glucose-based PD fluids. By inhibiting complement host defenses, glucose-based PD fluids may increase the risk of and severity of S. aureus peritonitis for CPD patients using these fluids.
[Mh] Termos MeSH primário: Soluções para Diálise/farmacologia
Glucose/farmacologia
Imunidade Inata/efeitos dos fármacos
Diálise Renal
Staphylococcus aureus/imunologia
[Mh] Termos MeSH secundário: Anafilatoxinas/imunologia
Complemento C3/imunologia
Via Alternativa do Complemento/efeitos dos fármacos
Seres Humanos
Proteínas Opsonizantes/metabolismo
Fagocitose/efeitos dos fármacos
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anaphylatoxins); 0 (Complement C3); 0 (Dialysis Solutions); 0 (Opsonin Proteins); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150909
[Lr] Data última revisão:
150909
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150811
[St] Status:MEDLINE


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[PMID]:26018222
[Au] Autor:Xu M; Liu B; Wu MF; Chen HT; Cianflone K; Wang ZL
[Ad] Endereço:Department of Obstetrics and Gynaecology, the First Affiliated Hospital of Sun Yat-sen University , Guangdong , China.
[Ti] Título:Relationships among acylation-stimulating protein, insulin resistance, lipometabolism, and fetal growth in gestational diabetes mellitus women.
[So] Source:J Obstet Gynaecol;35(4):341-5, 2015 May.
[Is] ISSN:1364-6893
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the potential relationship between acylation-stimulating protein (ASP), insulin resistance, lipometabolism, the intrauterine metabolic environment and fetal growth in well-controlled gestational diabetes mellitus (GDM) women. A total of 55 well-controlled GDM women, 66 pregnant women with normal glucose tolerance (NGT) and their newborns, were included in this study. Fasting maternal and cord blood ASP, serum lipid profiles, glucose level, insulin level, HOMA-IR, in addition to neonatal anthropometry data, were measured. Maternal blood ASP in GDM is higher than that in NGT. In the GDM group, maternal blood ASP has a positive correlation with TG, FFA and HOMA-IR. Maternal and cord blood ASP levels of LGA fetuses correlate with elevated birth weight and SF4. Similarly, cord blood ASP levels of LGA fetuses also correlate with birth weight and SF4 in the NGT group. The maternal blood ASP level of GDM mothers is associated with lipometabolism, insulin resistance and LGA fetal growth. Nevertheless, the cord blood ASP level correlates with FFA of GDM mothers, LGA fetal growth of GDM and NGT mothers. ASP may be a biomarker for evaluating insulin resistance of GDM and LGA fetal growth.
[Mh] Termos MeSH primário: Complemento C3a/metabolismo
Diabetes Gestacional
Sangue Fetal/metabolismo
Desenvolvimento Fetal
Resistência à Insulina
Metabolismo dos Lipídeos
[Mh] Termos MeSH secundário: Adulto
Anafilatoxinas/metabolismo
Peso ao Nascer
Glicemia/metabolismo
Índice de Massa Corporal
Estudos de Casos e Controles
China
Diabetes Gestacional/diagnóstico
Diabetes Gestacional/metabolismo
Feminino
Teste de Tolerância a Glucose/métodos
Seres Humanos
Recém-Nascido
Gravidez
Estatística como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anaphylatoxins); 0 (Blood Glucose); 0 (complement C3a, des-Arg-(77)-); 80295-42-7 (Complement C3a)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150529
[St] Status:MEDLINE
[do] DOI:10.3109/01443615.2014.960376


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[PMID]:25934649
[Au] Autor:Sakashita M; Yamada T; Imoto Y; Hirota T; Tamari M; Ito Y; Kubo S; Osawa Y; Takahashi N; Fujieda S
[Ad] Endereço:Department of Otorhinolaryngology, University of Fukui, Fukui 910-1193, Japan.
[Ti] Título:Long-term sublingual immunotherapy for Japanese cedar pollinosis and the levels of IL-17A and complement components 3a and 5a.
[So] Source:Cytokine;75(1):181-5, 2015 Sep.
[Is] ISSN:1096-0023
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Allergen-specific immunotherapy is the only treatment that can alter the natural course of allergic disease. We performed long-term sublingual immunotherapy (SLIT) for patients with seasonal allergic rhinitis caused by Japanese cedar pollen (SAR-JCP), screened molecules as candidate biomarkers, and investigated serum IL-17A and complement components 3a (C3a) and C5a in order to evaluate whether these molecules show changes correlated to symptom scores. In this study, we found that the long-term SLIT reduced the serum levels of IL-17A and C3a and C5a. The levels of C3a in the patients significantly decreased from year 1 compared with those at the baseline, and their levels of IL-17A significantly decreased from year 2 compared with those at baseline. The levels of IL-17A, C3a, and C5a at year 4 of SLIT were significantly lower than not only those at baseline, but also those at year 1. A significant positive correlation was found between the symptom medication scores and the levels of IL-17A at year 4. The symptom medication scores in the group in which IL-17A levels decreased at year 4 were significantly lower than those in the group without such a decrease. The serum level of IL-17A might prove useful as a biological parameter to ascertain the effectiveness of SLIT for patients with SAR-JCP. It is necessary to produce new therapeutics for non-responders in whom serum IL-17A levels are still higher against long-term SLIT.
[Mh] Termos MeSH primário: Complemento C3a/imunologia
Complemento C5a/imunologia
Dessensibilização Imunológica/métodos
Interleucina-17/sangue
Pólen/imunologia
Rinite Alérgica Sazonal/terapia
Imunoterapia Sublingual
[Mh] Termos MeSH secundário: Administração Sublingual
Adulto
Idoso
Alérgenos/imunologia
Anafilatoxinas
Cryptomeria
Ensaio de Imunoadsorção Enzimática
Feminino
Regulação da Expressão Gênica
Seres Humanos
Imunoterapia
Masculino
Meia-Idade
Rinite Alérgica/terapia
Rinite Alérgica Sazonal/imunologia
Fatores de Tempo
[Pt] Tipo de publicação:CLINICAL STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Allergens); 0 (Anaphylatoxins); 0 (IL17A protein, human); 0 (Interleukin-17); 80295-42-7 (Complement C3a); 80295-54-1 (Complement C5a)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150810
[Lr] Data última revisão:
150810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150503
[St] Status:MEDLINE


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[PMID]:25909494
[Au] Autor:Melillo D; Varriale S; Giacomelli S; Natale L; Bargelloni L; Oreste U; Pinto MR; Coscia MR
[Ad] Endereço:Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli (SZN), Italy.
[Ti] Título:Evolution of the complement system C3 gene in Antarctic teleosts.
[So] Source:Mol Immunol;66(2):299-309, 2015 Aug.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Notothenioidei are typical Antarctic teleosts evolved to adapt to the very low temperatures of the Antarctic seas. Aim of the present paper is to investigate sequence and structure of C3, the third component of the complement system of the notothenioid Trematomus bernacchii and Chionodraco hamatus. We determined the complete nucleotide sequence of two C3 isoforms of T. bernacchii and a single C3 isoform of C. hamatus. These sequences were aligned against other homologous teleost sequences to check for the presence of diversifying selection. Evidence for positive selection was observed in the evolutionary lineage of Antarctic teleost C3 sequences, especially in that of C. hamatus, the most recently diverged species. Adaptive selection affected numerous amino acid positions including three residues located in the anaphylatoxin domain. In an attempt to evaluate the link between sequence variants and specific structural features, we constructed molecular models of Antarctic teleost C3s, of their proteolytic fragments C3b and C3a, and of the corresponding molecules of the phylogenetically related temperate species Epinephelus coioides, using human crystallographic structures as templates. Subsequently, we compared dynamic features of these models by molecular dynamics simulations and found that the Antarctic C3s models show higher flexibility, which likely allows for more pronounced movements of both the TED domain in C3b and the carboxyl-terminal region of C3a. As such dynamic features are associated to positively selected sites, it appears that Antarctic teleost C3 molecules positively evolved toward an increased flexibility, to cope with low kinetic energy levels of the Antarctic marine environment.
[Mh] Termos MeSH primário: Anafilatoxinas/imunologia
Complemento C3/imunologia
Evolução Molecular
Proteínas de Peixes/imunologia
Perciformes/imunologia
Filogenia
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Adaptação Fisiológica/imunologia
Anafilatoxinas/química
Anafilatoxinas/genética
Animais
Regiões Antárticas
Sequência de Bases
Temperatura Baixa
Complemento C3/química
Complemento C3/genética
Proteínas de Peixes/química
Proteínas de Peixes/genética
Expressão Gênica
Seres Humanos
Simulação de Dinâmica Molecular
Dados de Sequência Molecular
Perciformes/classificação
Perciformes/genética
Isoformas de Proteínas/química
Isoformas de Proteínas/genética
Isoformas de Proteínas/imunologia
Estrutura Terciária de Proteína
Proteólise
Seleção Genética
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anaphylatoxins); 0 (Complement C3); 0 (Fish Proteins); 0 (Protein Isoforms)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:150608
[Lr] Data última revisão:
150608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150425
[St] Status:MEDLINE



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