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[PMID]:29329339
[Au] Autor:Granger BL
[Ad] Endereço:Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, United States of America.
[Ti] Título:Accessibility and contribution to glucan masking of natural and genetically tagged versions of yeast wall protein 1 of Candida albicans.
[So] Source:PLoS One;13(1):e0191194, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Yeast wall protein 1 (Ywp1) is an abundant glycoprotein of the cell wall of the yeast form of Candida albicans, the most prevalent fungal pathogen of humans. Antibodies that bind to the polypeptide backbone of isolated Ywp1 show little binding to intact yeast cells, presumably because the Ywp1 epitopes are masked by the polysaccharides of the mannoproteins that form the outer layer of the cell wall. Rare cells do exhibit much greater anti-Ywp1 binding, however, and one of these was isolated and characterized. No differences were seen in its Ywp1, but it exhibited greater adhesiveness, sensitivity to wall perturbing agents, and exposure of its underlying ß-1,3-glucan layer to external antibodies. The molecular basis for this greater epitope accessibility has not been determined, but has facilitated exploration of how these properties change as a function of cell growth and morphology. In addition, previously engineered strains with reduced quantities of Ywp1 in their cell walls were also found to have greater ß-1,3-glucan exposure, indicating that Ywp1 itself contributes to the masking of wall epitopes, which may be important for understanding the anti-adhesive effect of Ywp1. Ectopic production of Ywp1 by hyphae, which reduces the adhesivity of these filamentous forms of C. albicans, was similarly found to reduce exposure of the ß-1,3-glucan in their walls. To monitor Ywp1 in the cell wall irrespective of its accessibility, green fluorescent protein (Gfp) was genetically inserted into wall-anchored Ywp1 using a bifunctional cassette that also allowed production from a single transfection of a soluble, anchor-free version. The wall-anchored Ywp1-Gfp-Ywp1 accumulated in the wall of the yeast forms but not hyphae, and appeared to have properties similar to native Ywp1, including its adhesion-inhibiting effect. Some pseudohyphal walls also detectably accumulated this probe. Strains of C. albicans with tandem hemagglutinin (HA) epitopes inserted into wall-anchored Ywp1 were previously created by others, and were further explored here. As above, rare cells with much greater accessibility of the HA epitopes were isolated, and also found to exhibit greater exposure of Ywp1 and ß-1,3-glucan. The placement of the HA cassette inhibited the normal N-glycosylation and propeptide cleavage of Ywp1, but the wall-anchored Ywp1-HA-Ywp1 still accumulated in the cell wall of yeast forms. Bifunctional transformation cassettes were used to additionally tag these molecules with Gfp, generating soluble Ywp1-HA-Gfp and wall-anchored Ywp1-HA-Gfp-Ywp1 molecules. The former revealed unexpected electrophoretic properties caused by the HA insertion, while the latter further highlighted differences between the presence of a tagged Ywp1 molecule (as revealed by Gfp fluorescence) and its accessibility in the cell wall to externally applied antibodies specific for HA, Gfp and Ywp1, with accessibility being greatest in the rapidly expanding walls of budding daughter cells. These strains and results increase our understanding of cell wall properties and how C. albicans masks itself from recognition by the human immune system.
[Mh] Termos MeSH primário: Candida albicans/genética
Candida albicans/metabolismo
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
beta-Glucanas/metabolismo
[Mh] Termos MeSH secundário: Anticorpos Antifúngicos
Antígenos de Fungos/química
Antígenos de Fungos/genética
Antígenos de Fungos/metabolismo
Candida albicans/imunologia
Parede Celular/genética
Parede Celular/imunologia
Parede Celular/metabolismo
Epitopos/química
Epitopos/genética
Epitopos/metabolismo
Proteínas Fúngicas/imunologia
Glicosilação
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/imunologia
Proteínas de Fluorescência Verde/metabolismo
Hemaglutininas/genética
Hemaglutininas/imunologia
Hemaglutininas/metabolismo
Seres Humanos
Glicoproteínas de Membrana/genética
Glicoproteínas de Membrana/imunologia
Glicoproteínas de Membrana/metabolismo
Engenharia de Proteínas
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/imunologia
Proteínas Recombinantes de Fusão/metabolismo
beta-Glucanas/química
beta-Glucanas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (Antigens, Fungal); 0 (Epitopes); 0 (Fungal Proteins); 0 (Hemagglutinins); 0 (Membrane Glycoproteins); 0 (Recombinant Fusion Proteins); 0 (beta-Glucans); 0 (mannoproteins); 147336-22-9 (Green Fluorescent Proteins); 9051-97-2 (beta-1,3-glucan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191194


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[PMID]:29326275
[Au] Autor:Leonardi I; Li X; Semon A; Li D; Doron I; Putzel G; Bar A; Prieto D; Rescigno M; McGovern DPB; Pla J; Iliev ID
[Ad] Endereço:Gastroenterology and Hepatology Division, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA.
[Ti] Título:CX3CR1 mononuclear phagocytes control immunity to intestinal fungi.
[So] Source:Science;359(6372):232-236, 2018 01 12.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intestinal fungi are an important component of the microbiota, and recent studies have unveiled their potential in modulating host immune homeostasis and inflammatory disease. Nonetheless, the mechanisms governing immunity to gut fungal communities (mycobiota) remain unknown. We identified CX3CR1 mononuclear phagocytes (MNPs) as being essential for the initiation of innate and adaptive immune responses to intestinal fungi. CX3CR1 MNPs express antifungal receptors and activate antifungal responses in a Syk-dependent manner. Genetic ablation of CX3CR1 MNPs in mice led to changes in gut fungal communities and to severe colitis that was rescued by antifungal treatment. In Crohn's disease patients, a missense mutation in the gene encoding CX3CR1 was identified and found to be associated with impaired antifungal responses. These results unravel a role of CX3CR1 MNPs in mediating interactions between intestinal mycobiota and host immunity at steady state and during inflammatory disease.
[Mh] Termos MeSH primário: Receptor 1 de Quimiocina CX3C/análise
Receptor 1 de Quimiocina CX3C/genética
Candida albicans/imunologia
Microbioma Gastrointestinal/imunologia
Intestinos/microbiologia
Micobioma/imunologia
Fagócitos/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antifúngicos/biossíntese
Anticorpos Antifúngicos/sangue
Candida albicans/crescimento & desenvolvimento
Colite/tratamento farmacológico
Colite/microbiologia
Doença de Crohn/genética
Doença de Crohn/imunologia
Células Dendríticas/imunologia
Microbioma Gastrointestinal/fisiologia
Seres Humanos
Imunidade nas Mucosas
Imunoglobulina G/biossíntese
Imunoglobulina G/sangue
Intestinos/imunologia
Camundongos
Mutação de Sentido Incorreto
Micobioma/fisiologia
Fagócitos/microbiologia
Linfócitos T Reguladores/imunologia
Células Th17/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (CX3C Chemokine Receptor 1); 0 (CX3CR1 protein, human); 0 (Cx3cr1 protein, mouse); 0 (Immunoglobulin G)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1126/science.aao1503


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[PMID]:28945819
[Au] Autor:Weissenbacher-Lang C; Nedorost N; Knecht C; Hennig-Pauka I; Huber M; Voglmayr T; Weissenböck H
[Ad] Endereço:Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.
[Ti] Título:Comparison of Pneumocystis nucleic acid and antibody profiles and their associations with other respiratory pathogens in two Austrian pig herds.
[So] Source:PLoS One;12(9):e0185387, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pneumocystis carinii f. sp. suis (PCS) nucleic acid and antibody profiles on two Austrian-farrow-to-finish farms were investigated. Furthermore, associations with other respiratory pathogens were evaluated. Respiratory specimen and sera from pigs of five age classes between the 1st week and the 3rd month of life as well as samples from sows were analyzed. On Farm A, PCS infection occurred early in life. The suckling piglets were already infected in the 1st week of life and the pigs remained positive until the 3rd month of life. On Farm B, pigs were infected later, between 3 and 4 months of age. The maximum PCS nucleic acid load on Farm A was 8.3 log10 genome copies/mL BALF, whereas on Farm B the PCS burden was significantly lower, with 4.0 log10 genome copies/mL BALF. Anti-PCS antibodies were detected in sows, as maternal antibodies in suckling piglets and as an immunological reaction to infection. On both farms, PCS infection was accompanied by several co-infections. On Farm A, there were concurrent infections with PRRSV, a virulent strain of Haemophilus parasuis, and Mycoplasma hyopneumoniae. On Farm B, PCS was accompanied by infections with swine influenza virus, Mycoplasma hyopneumoniae, and a non-virulent strain of Haemophilus parasuis. The results clearly show that the PCS profiles can vary between farms. Younger pigs may be more susceptible as they had higher PCS burdens. It is possible that PCS may contribute to a respiratory disease in pigs and further investigation of its potential role is warranted.
[Mh] Termos MeSH primário: Pneumocystis carinii/patogenicidade
Pneumonia por Pneumocystis/veterinária
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Animais Recém-Nascidos
Anticorpos Antifúngicos/análise
Anticorpos Antifúngicos/sangue
Áustria
Coinfecção/imunologia
Coinfecção/microbiologia
Coinfecção/veterinária
Estudos Transversais
DNA Bacteriano/análise
DNA Bacteriano/sangue
DNA Bacteriano/genética
DNA Fúngico/análise
DNA Fúngico/sangue
DNA Fúngico/genética
DNA Viral/análise
DNA Viral/sangue
DNA Viral/genética
Feminino
Infecções por Haemophilus/imunologia
Infecções por Haemophilus/microbiologia
Infecções por Haemophilus/veterinária
Haemophilus parasuis/genética
Haemophilus parasuis/isolamento & purificação
Masculino
Infecções por Orthomyxoviridae/imunologia
Infecções por Orthomyxoviridae/microbiologia
Infecções por Orthomyxoviridae/veterinária
Pneumocystis carinii/genética
Pneumocystis carinii/imunologia
Pneumonia Suína Micoplasmática/imunologia
Pneumonia Suína Micoplasmática/microbiologia
Pneumonia por Pneumocystis/imunologia
Pneumonia por Pneumocystis/microbiologia
Síndrome Respiratória e Reprodutiva Suína/imunologia
Síndrome Respiratória e Reprodutiva Suína/microbiologia
Síndrome Respiratória e Reprodutiva Suína/virologia
Sus scrofa
Suínos
Doenças dos Suínos/imunologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (DNA, Bacterial); 0 (DNA, Fungal); 0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185387


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Burger, Eva
Texto completo
[PMID]:28854184
[Au] Autor:Della Terra PP; Rodrigues AM; Fernandes GF; Nishikaku AS; Burger E; de Camargo ZP
[Ad] Endereço:Department of Medicine, Discipline of Infectious Diseases, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
[Ti] Título:Exploring virulence and immunogenicity in the emerging pathogen Sporothrix brasiliensis.
[So] Source:PLoS Negl Trop Dis;11(8):e0005903, 2017 Aug.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sporotrichosis is a polymorphic chronic infection of humans and animals classically acquired after traumatic inoculation with soil and plant material contaminated with Sporothrix spp. propagules. An alternative and successful route of transmission is bites and scratches from diseased cats, through which Sporothrix yeasts are inoculated into mammalian tissue. The development of a murine model of subcutaneous sporotrichosis mimicking the alternative route of transmission is essential to understanding disease pathogenesis and the development of novel therapeutic strategies. To explore the impact of horizontal transmission in animals (e.g., cat-cat) and zoonotic transmission on Sporothrix fitness, the left hind footpads of BALB/c mice were inoculated with 5×106 yeasts (n = 11 S. brasiliensis, n = 2 S. schenckii, or n = 1 S. globosa). Twenty days post-infection, our model reproduced both the pathophysiology and symptomology of sporotrichosis with suppurating subcutaneous nodules that progressed proximally along lymphatic channels. Across the main pathogenic members of the S. schenckii clade, S. brasiliensis was usually more virulent than S. schenckii and S. globosa. However, the virulence in S. brasiliensis was strain-dependent, and we demonstrated that highly virulent isolates disseminate from the left hind footpad to the liver, spleen, kidneys, lungs, heart, and brain of infected animals, inducing significant and chronic weight loss (losing up to 15% of their body weight). The weight loss correlated with host death between 2 and 16 weeks post-infection. Histopathological features included necrosis, suppurative inflammation, and polymorphonuclear and mononuclear inflammatory infiltrates. Immunoblot using specific antisera and homologous exoantigen investigated the humoral response. Antigenic profiles were isolate-specific, supporting the hypothesis that different Sporothrix species can elicit a heterogeneous humoral response over time, but cross reaction was observed between S. brasiliensis and S. schenckii proteomes. Despite great diversity in the immunoblot profiles, antibodies were mainly derived against 3-carboxymuconate cyclase, a glycoprotein oscillating between 60 and 70 kDa (gp60-gp70) and a 100-kDa molecule in nearly 100% of the assays. Thus, our data broaden the current view of virulence and immunogenicity in the Sporothrix-sporotrichosis system, substantially expanding the possibilities for comparative genomic with isolates bearing divergent virulence traits and helping uncover the molecular mechanisms and evolutionary pressures underpinning the emergence of Sporothrix virulence.
[Mh] Termos MeSH primário: Sporothrix/imunologia
Sporothrix/patogenicidade
Esporotricose/imunologia
Esporotricose/patologia
[Mh] Termos MeSH secundário: Estruturas Animais/microbiologia
Estruturas Animais/patologia
Animais
Anticorpos Antifúngicos/sangue
Antígenos de Fungos/imunologia
Peso Corporal
Modelos Animais de Doenças
Histocitoquímica
Immunoblotting
Camundongos Endogâmicos BALB C
Análise de Sobrevida
Fatores de Tempo
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (Antigens, Fungal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005903


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[PMID]:28749993
[Au] Autor:Chen G; Wang W; Chen H; Dai W; Peng X; Li X; Tang X; Xu L; Shen Z
[Ad] Endereço:Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province, China.
[Ti] Título:Functional characterization of an aquaporin from a microsporidium, Nosema bombycis.
[So] Source:PLoS One;12(7):e0181703, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microsporidia are a diverse group of eukaryotic organisms, capable of causing parasitic infections in both vertebrates and invertebrates. During the germination process, there is an increase in the osmotic pressure of microsporidian spores. As part of this study, we cloned a homologous aquaporin gene in Nosema bombycis, and named it Nosema bombycis aquaporin (NbAQP). Sequence analysis revealed that the NbAQP contains an open reading frame with a length of 750 bp and encodes a polypeptide of 249 amino acids. Amino acid sequence homology was greater than 50% that of five aquaporins from other microsporidian species. Indirect immunofluorescence (IFA) and immunogold electron microscopy showed NbAQP to be located predominantly in the spore wall of N. bombycis spores. The results of qRT-PCR analysis revealed that NbAQP expression remained high 0 h after inoculation and decreased sharply to 24 h, increased gradually from 2 days and peaked at 6 days. After expression of NbAQP in Xenopus laevis oocytes, it was observed that NbAQP can promote rapid penetration of water into oocytes. The associated permeation rate was 2-3 times that of the water-injected and uninjected oocytes. Antibody blocking experiments showed that the inhibition rate of spore germination was approximately 28% after antibody blocking. The difference in germination rate between the control group and the NbAQP group was significant (P < 0.05). This study shows for the first time that N. bombycis contains functional water channel proteins and provides a platform suitable for further research into the mechanisms underlying the regulation of NbAQP protein expression. Further study of NbAQP and their inhibitors may have significance for prevention of microsporidiosis.
[Mh] Termos MeSH primário: Aquaporinas/fisiologia
Proteínas Fúngicas/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anticorpos Antifúngicos/farmacologia
Antifúngicos/farmacologia
Aquaporinas/antagonistas & inibidores
Células Cultivadas
Proteínas Fúngicas/antagonistas & inibidores
Modelos Moleculares
Nosema/efeitos dos fármacos
Nosema/metabolismo
Filogenia
Domínios Proteicos
Coelhos
Homologia de Sequência de Aminoácidos
Esporos Fúngicos/efeitos dos fármacos
Esporos Fúngicos/metabolismo
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (Antifungal Agents); 0 (Aquaporins); 0 (Fungal Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181703


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[PMID]:28547648
[Au] Autor:Rinawi F; Assa A; Bashir H; Peleg S; Shamir R
[Ad] Endereço:Institute of Gastroenterology, Nutrition and Liver Diseases, Schneider Children's Medical Center of Israel, 14 Kaplan St., 49202, Petach Tikva, Israel. firasytr@gmail.com.
[Ti] Título:Clinical and Phenotypic Differences in Inflammatory Bowel Disease Among Arab and Jewish Children in Israel.
[So] Source:Dig Dis Sci;62(8):2095-2101, 2017 Aug.
[Is] ISSN:1573-2568
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Data on inflammatory bowel disease (IBD) phenotypes among the Arab population in Israel or in the neighboring Arab countries is scarce. AIM: We aimed to assess differences in disease phenotype among Arab and Jewish children living in Israel. METHODS: We performed a retrospective chart review of pediatric IBD cases, which were diagnosed at the Schneider Children's Medical Center and Ha'Emek Medical Center in Israel between 2000 and 2014. Demographic, clinical, and phenotypic variables were compared between Arabs and Jews from Eastern (Sephardic) and Western (Ashkenazi) origin. RESULTS: Seventy-one Arab children with IBD were compared with 165 Ashkenazi and 158 Sephardic Jewish children. Age and gender did not differ between groups. Sephardic and Ashkenazi Jewish Crohn's disease (CD) patients had significantly more stenotic behavior (24 and 26 vs. 5%, p = 0.03) and less fistulzing perianal disease (15 and 11 vs. 31%, p = 0.014) compared with Arab patients. Arab children with ulcerative colitis (UC) had more severe disease at diagnosis compared to Sephardic and Ashkenazi Jews reflected by higher Pediatric UC Activity Index (45 vs. 35 and 35, respectively, p = 0.03). Arab patients had significantly lower proportion of anti-Saccharomyces cerevisiae antibodies positivity (in CD) and perinuclear anti-neutrophil cytoplasmic antibodies positivity (in UC) than both Sephardic and Ashkenazi Jewish children (23 vs. 53 and 65%, p = 0.002 and 35 vs. 60 and 75%, respectively, p = 0.002). CONCLUSION: Arab and Jewish children with IBD differ in disease characteristics and severity. Whether genetic or environmental factors are the cause for these differences is yet to be determined.
[Mh] Termos MeSH primário: Árabes/estatística & dados numéricos
Colite Ulcerativa/etnologia
Doença de Crohn/etnologia
Judeus/estatística & dados numéricos
Fenótipo
[Mh] Termos MeSH secundário: Adolescente
Anticorpos Anticitoplasma de Neutrófilos/sangue
Anticorpos Antifúngicos/sangue
Criança
Pré-Escolar
Colite Ulcerativa/sangue
Colite Ulcerativa/patologia
Doença de Crohn/sangue
Doença de Crohn/patologia
Feminino
Seres Humanos
Israel
Masculino
Estudos Retrospectivos
Saccharomyces cerevisiae/imunologia
Índice de Gravidade de Doença
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Antineutrophil Cytoplasmic); 0 (Antibodies, Fungal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1007/s10620-017-4623-x


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[PMID]:28426751
[Au] Autor:Han B; Polonais V; Sugi T; Yakubu R; Takvorian PM; Cali A; Maier K; Long M; Levy M; Tanowitz HB; Pan G; Delbac F; Zhou Z; Weiss LM
[Ad] Endereço:State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, P. R. China.
[Ti] Título:The role of microsporidian polar tube protein 4 (PTP4) in host cell infection.
[So] Source:PLoS Pathog;13(4):e1006341, 2017 Apr.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microsporidia have been identified as pathogens that have important effects on our health, food security and economy. A key to the success of these obligate intracellular pathogens is their unique invasion organelle, the polar tube, which delivers the nucleus containing sporoplasm into host cells during invasion. Due to the size of the polar tube, the rapidity of polar tube discharge and sporoplasm passage, and the absence of genetic techniques for the manipulation of microsporidia, study of this organelle has been difficult and there is relatively little known regarding polar tube formation and the function of the proteins making up this structure. Herein, we have characterized polar tube protein 4 (PTP4) from the microsporidium Encephalitozoon hellem and found that a monoclonal antibody to PTP4 labels the tip of the polar tube suggesting that PTP4 might be involved in a direct interaction with host cell proteins during invasion. Further analyses employing indirect immunofluorescence (IFA), enzyme-linked immunosorbent (ELISA) and fluorescence-activated cell sorting (FACS) assays confirmed that PTP4 binds to mammalian cells. The addition of either recombinant PTP4 protein or anti-PTP4 antibody reduced microsporidian infection of its host cells in vitro. Proteomic analysis of PTP4 bound to host cell membranes purified by immunoprecipitation identified transferrin receptor 1 (TfR1) as a potential host cell interacting partner for PTP4. Additional experiments revealed that knocking out TfR1, adding TfR1 recombinant protein into cell culture, or adding anti-TfR1 antibody into cell culture significantly reduced microsporidian infection rates. These results indicate that PTP4 is an important protein competent of the polar tube involved in the mechanism of host cell infection utilized by these pathogens.
[Mh] Termos MeSH primário: Anticorpos Antifúngicos/imunologia
Encephalitozoon/genética
Encefalitozoonose/microbiologia
Proteínas Fúngicas/metabolismo
Proteômica
[Mh] Termos MeSH secundário: Animais
Membrana Celular/metabolismo
Cricetinae
Cricetulus
Encephalitozoon/imunologia
Encephalitozoon/patogenicidade
Encephalitozoon/ultraestrutura
Encefalitozoonose/patologia
Proteínas Fúngicas/genética
Organelas/metabolismo
Organelas/ultraestrutura
Coelhos
Receptores da Transferrina/genética
Receptores da Transferrina/metabolismo
Proteínas Recombinantes
Esporos Fúngicos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (Fungal Proteins); 0 (PTP protein, Encephalitozoon hellem); 0 (Receptors, Transferrin); 0 (Recombinant Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006341


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[PMID]:28380017
[Au] Autor:Chow NA; Lindsley MD; McCotter OZ; Kangiser D; Wohrle RD; Clifford WR; Yaglom HD; Adams LE; Komatsu K; Durkin MM; Baker RJ; Shubitz LF; Derado G; Chiller TM; Litvintseva AP
[Ad] Endereço:Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
[Ti] Título:Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species.
[So] Source:PLoS One;12(4):e0175081, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coccidioides is a soil-dwelling fungus that causes coccidioidomycosis, a disease also known as Valley fever, which affects humans and a variety of animal species. Recent findings of Coccidioides in new, unexpected areas of the United States have demonstrated the need for a better understanding of its geographic distribution. Large serological studies on animals could provide important information on the geographic distribution of this pathogen. To facilitate such studies, we used protein A/G, a recombinant protein that binds IgG antibodies from a variety of mammalian species, to develop an enzyme immunoassay (EIA) that detects IgG antibodies against Coccidioides in a highly sensitive and high-throughput manner. We showed the potential of this assay to be adapted to multiple animal species by testing a collection of serum and/or plasma samples from dogs, mice, and humans with or without confirmed coccidioidomycosis. We then evaluated the performance of the assay in dogs, using sera from dogs residing in a highly endemic area, and found seropositivity rates significantly higher than those in dogs of non-endemic areas. We further evaluated the specificity of the assay in dogs infected with other fungal pathogens known to cross-react with Coccidioides. Finally, we used the assay to perform a cross-sectional serosurvey investigating dogs from Washington, a state in which infection with Coccidioides has recently been documented. In summary, we have developed a Coccidioides EIA for the detection of antibodies in canines that is more sensitive and has higher throughput than currently available methods, and by testing this assay in mice and humans, we have shown a proof of principle of its adaptability for other animal species.
[Mh] Termos MeSH primário: Anticorpos Antifúngicos/imunologia
Coccidioides/imunologia
Coccidioidomicose/veterinária
Doenças do Cão/diagnóstico
Técnicas Imunoenzimáticas/veterinária
[Mh] Termos MeSH secundário: Animais
Coccidioidomicose/diagnóstico
Coccidioidomicose/epidemiologia
Coccidioidomicose/imunologia
Estudos Transversais
Doenças do Cão/epidemiologia
Doenças do Cão/imunologia
Doenças do Cão/microbiologia
Cães
Imunodifusão/veterinária
Técnicas Imunoenzimáticas/métodos
Washington/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175081


  9 / 3687 MEDLINE  
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[PMID]:28329526
[Au] Autor:Carvalho S; Machado S; Sampaio R; Guedes M; Vasconcelos J; Semedo D; Selores M
[Ad] Endereço:Department of Dermatology, Centro Hospitalar do Porto, Oporto, Portugal. carvalhosandrine@gmail.com.
[Ti] Título:Chronic granulomatous disease as a risk factor for cutaneous lupus in childhood.
[So] Source:Dermatol Online J;23(3), 2017 Mar 15.
[Is] ISSN:1087-2108
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic granulomatous disease (CGD) is a primaryimmunodeficiency disorder that affects the phagocyticcells of the innate immune system. It is characterizedby recurrent or persistent infections with granulomaformation. Lupus-like lesions have been reported incarriers of CGD and less frequently, in patients withCGD. Immunological study in these patients areusually negative. We describe the case of an 8-yearoldboy with CGD who developed chronic and acutecutaneous lupus erythematous with angular cheilitis,oral ulcers, Raynaud phenomenon, and positiveserologies for antinuclear, anticentromere, and anti-Saccharomyces cerevisiae antibodies.
[Mh] Termos MeSH primário: Dermatoses Faciais/diagnóstico
Dermatoses do Pé/diagnóstico
Doença Granulomatosa Crônica/imunologia
Lúpus Eritematoso Cutâneo/diagnóstico
[Mh] Termos MeSH secundário: Anticorpos Antinucleares/imunologia
Anticorpos Antifúngicos/imunologia
Queilite/complicações
Queilite/diagnóstico
Queilite/imunologia
Criança
Dermatoses Faciais/complicações
Dermatoses Faciais/imunologia
Dermatoses Faciais/patologia
Dermatoses do Pé/complicações
Dermatoses do Pé/imunologia
Dermatoses do Pé/patologia
Doença Granulomatosa Crônica/complicações
Seres Humanos
Lúpus Eritematoso Cutâneo/complicações
Lúpus Eritematoso Cutâneo/imunologia
Lúpus Eritematoso Cutâneo/patologia
Masculino
Úlceras Orais/complicações
Úlceras Orais/diagnóstico
Úlceras Orais/imunologia
Doença de Raynaud/complicações
Doença de Raynaud/diagnóstico
Doença de Raynaud/imunologia
Fatores de Risco
Saccharomyces cerevisiae/imunologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Antinuclear); 0 (Antibodies, Fungal); 0 (anticentromere antibody)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE


  10 / 3687 MEDLINE  
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[PMID]:28295653
[Au] Autor:Mendes JF; Klafke GB; Albano APN; Cabana ÂL; Teles AJ; de Camargo ZP; Xavier MO; Meireles MCA
[Ad] Endereço:Center of Diagnosis in Veterinary Mycology, Department of Veterinary Preventive, Faculty of Veterinary, University Federal of Pelotas (UFPel), Pelotas, RS, Brazil.
[Ti] Título:Paracoccidioidomycosis infection in domestic and wild mammals by Paracoccidioides lutzii.
[So] Source:Mycoses;60(6):402-406, 2017 Jun.
[Is] ISSN:1439-0507
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Paracoccidioidomycosis (PCM) is a systemic mycosis that occurs in several Latin American countries, especially in Brazil. It is caused by the thermo-dimorphic fungus Paracoccidioides spp. Serological studies to detect animal infection represent an excellent strategy for data on the agent's ecology. Although the state of Rio Grande do Sul (RS) is an endemic area for PCM in humans, there is scarce information available on the ecology of the agent in the region. This study aimed to investigate the infection by Paracoccidioides lutzii in animals living in RS, Brazil. A total of 85 wild mammals, 200 horses and 196 domestic dogs, previously tested for infection by P. brasiliensis, were included in this study. Serum samples from the animals were tested by ELISA to detect anti- P. lutzii antibodies. From the 481 animals tested, 105 (21.8%) were seropositive for IgG anti-P. lutzii. Of these, 54 were also positive for P. brasiliensis. A total of 11 horses (10.5%), 30 dogs (28.8%) and 10 wild mammals (9.5%) were positive only for P. lutzii (n=51). The detection of anti-P. lutzii antibodies in animals of RS suggests that the fungus can be found in southern Brazil, despite being described mainly in the midwest and southeast of the country.
[Mh] Termos MeSH primário: Mamíferos/microbiologia
Paracoccidioides/isolamento & purificação
Paracoccidioidomicose/veterinária
[Mh] Termos MeSH secundário: Animais
Anticorpos Antifúngicos/sangue
Antígenos de Fungos/imunologia
Brasil/epidemiologia
Cães/microbiologia
Ensaio de Imunoadsorção Enzimática
Cavalos/microbiologia
Seres Humanos
Paracoccidioidomicose/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (Antigens, Fungal)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1111/myc.12608



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