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[PMID]:28453850
[Au] Autor:Mukherjee S; Mukherjee S; Maiti TK; Bhattacharya S; Sinha Babu SP
[Ad] Endereço:Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, West Bengal, India.
[Ti] Título:A Novel Ligand of Toll-like Receptor 4 From the Sheath of Wuchereria bancrofti Microfilaria Induces Proinflammatory Response in Macrophages.
[So] Source:J Infect Dis;215(6):954-965, 2017 03 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Lymphatic filariasis, frequently caused from Wuchereria bancrofti infection, is endemic in several parts of the globe and responsible for human health problems and socioeconomic loss to a large extent. Inflammatory consequences originating from host-parasite interaction play a major role in the disease pathology and allied complications. The identity of the key mediator of this process is yet unknown in filarial research. Methods: Microfilarial protein (MfP) was isolated from the sheath of W. bancrofti microfilariae through ultrafiltration, followed by chromatographic separation. Expression of signaling molecules was studied by enzyme-linked immunosorbent assay and immunoblotting. Binding of MfP to Toll-like receptor 4 (TLR4) was determined by co-immunoprecipitation, fluorescein isothiocyanate-probing, and surface plasmon resonance analysis. Results: We found that MfP (approximately 70 kDa) binds to macrophage-TLR4 and triggers nuclear factor kappa beta activation that upregulates secretion of proinflammatory cytokines. Microfilarial protein failed to induce inflammation in either TLRKO macrophage or macrophage treated with TLR4 inhibitor, indicating that MfP acts through TLR4. We have also detected phenotypic transformation of macrophages from anti-inflammatory (M2) to proinflammatory (M1) subtype after incubation with MfP. Conclusions: Microfilarial protein appears to be a new ligand of TLR4 from W. bancrofti. Determination of its functional attributions in the host-parasite relationship, especially immunopathogenesis of filarial infection, may improve our understanding.
[Mh] Termos MeSH primário: Antígenos de Helmintos/imunologia
Proteínas de Helminto/imunologia
Macrófagos/imunologia
Receptor 4 Toll-Like/imunologia
Wuchereria bancrofti/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Anti-Helmínticos/sangue
Células Cultivadas
Ensaio de Imunoadsorção Enzimática
Células HEK293
Interações Hospedeiro-Parasita
Seres Humanos
Ligantes
Camundongos
Camundongos Endogâmicos BALB C
Microfilárias/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Helminth Proteins); 0 (Ligands); 0 (Toll-Like Receptor 4)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix067


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[PMID]:29368826
[Au] Autor:Lauffer A; Lutz P; Flesher SL
[Ti] Título:Baylisascaris Procyonis Exposure Case Study.
[So] Source:W V Med J;112(6):32-3, 2016 Nov-Dec.
[Is] ISSN:0043-3284
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We report a case of exposure to raccoon feces found to be contaminated with baylisascaris procyonis. The exposure was recognized early enough by the family to allow prophylaxis with albendazole. Because of the potential fatal or neurologically catastrophic effects of this disease immediate treatment is indicated. This is started in advance of environmental studies that are done to determine if the feces is indeed contaminated.
[Mh] Termos MeSH primário: Albendazol
Anti-Helmínticos
Infecções por Ascaridida/veterinária
Ascaridídios
Guaxinins/parasitologia
[Mh] Termos MeSH secundário: Adulto
Albendazol/uso terapêutico
Animais
Anti-Helmínticos/uso terapêutico
Anticorpos Anti-Helmínticos/sangue
Ascaridídios/imunologia
Ascaridídios/isolamento & purificação
Infecções por Ascaridida/sangue
Infecções por Ascaridida/diagnóstico
Infecções por Ascaridida/tratamento farmacológico
Criança
Fezes/parasitologia
Seres Humanos
Masculino
Fatores de Risco
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Antibodies, Helminth); F4216019LN (Albendazole)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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Registro de Ensaios Clínicos
Registro de Ensaios Clínicos
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[PMID]:28464026
[Au] Autor:Diemert DJ; Freire J; Valente V; Fraga CG; Talles F; Grahek S; Campbell D; Jariwala A; Periago MV; Enk M; Gazzinelli MF; Bottazzi ME; Hamilton R; Brelsford J; Yakovleva A; Li G; Peng J; Correa-Oliveira R; Hotez P; Bethony J
[Ad] Endereço:Department of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, The George Washington University, Washington DC, United States of America.
[Ti] Título:Safety and immunogenicity of the Na-GST-1 hookworm vaccine in Brazilian and American adults.
[So] Source:PLoS Negl Trop Dis;11(5):e0005574, 2017 05.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Necator americanus Glutathione-S-Transferase-1 (Na-GST-1) plays a role in the digestion of host hemoglobin by adult N. americanus hookworms. Vaccination of laboratory animals with recombinant Na-GST-1 is associated with significant protection from challenge infection. Recombinant Na-GST-1 was expressed in Pichia pastoris and adsorbed to aluminum hydroxide adjuvant (Alhydrogel) according to current Good Manufacturing Practice. Two Phase 1 trials were conducted in 142 healthy adult volunteers in the United States and Brazil, first in hookworm-naïve individuals and then in residents of a N. americanus endemic area in Brazil. Volunteers received one of three doses of recombinant Na-GST-1 (10, 30, or 100 µg) adjuvanted with Alhydrogel, adjuvanted with Alhydrogel and co-administered with an aqueous formulation of Glucopyranosyl Lipid A (GLA-AF), or the hepatitis B vaccine. Vaccinations were administered via intramuscular injection on days 0, 56, and 112. Na-GST-1/Alhydrogel was well tolerated in both hookworm-naïve and hookworm-exposed adults, with the most common adverse events being mild to moderate injection site pain and tenderness, and mild headache and nausea; no vaccine-related severe or serious adverse events were observed. Antigen-specific IgG antibodies were induced in a dose-dependent fashion, with increasing levels observed after each vaccination in both trials. The addition of GLA-AF to Na-GST-1/Alhydrogel did not result in significant increases in specific IgG responses. In both the US and Brazil studies, the predominant IgG subclass induced against Na-GST-1 was IgG1, with lesser amounts of IgG3. Vaccination of both hookworm-naïve and hookworm-exposed adults with recombinant Na-GST-1 was safe, well tolerated, and resulted in significant antigen-specific IgG responses. Based on these results, this vaccine will be advanced into clinical trials in children and eventual efficacy studies. TRIAL REGISTRATION: ClinicalTrials.gov (NCT01261130 for the Brazil trial and NCT01385189 for the US trial).
[Mh] Termos MeSH primário: Ancylostomatoidea/imunologia
Antígenos de Helmintos/imunologia
Glutationa Transferase/imunologia
Infecções por Uncinaria/prevenção & controle
Vacinas Sintéticas/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Adolescente
Adulto
Hidróxido de Alumínio/administração & dosagem
Animais
Anticorpos Anti-Helmínticos/sangue
Brasil
Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/epidemiologia
Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/patologia
Feminino
Glucosídeos/administração & dosagem
Voluntários Saudáveis
Vacinas contra Hepatite B/administração & dosagem
Infecções por Uncinaria/imunologia
Seres Humanos
Imunoglobulina G/sangue
Injeções Intramusculares
Lipídeo A/administração & dosagem
Masculino
Meia-Idade
Resultado do Tratamento
Estados Unidos
Vacinas Sintéticas/administração & dosagem
Vacinas Sintéticas/efeitos adversos
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE I; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Glucosides); 0 (Hepatitis B Vaccines); 0 (Immunoglobulin G); 0 (Lipid A); 0 (Vaccines, Synthetic); 0 (glucopyranosyl lipid-A); 5QB0T2IUN0 (Aluminum Hydroxide); EC 2.5.1.18 (Glutathione Transferase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005574


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[PMID]:28972982
[Au] Autor:Dieye Y; Storey HL; Barrett KL; Gerth-Guyette E; Di Giorgio L; Golden A; Faulx D; Kalnoky M; Ndiaye MKN; Sy N; Mané M; Faye B; Sarr M; Dioukhane EM; Peck RB; Guinot P; de Los Santos T
[Ad] Endereço:PATH, Seattle, Washington, United States of America.
[Ti] Título:Feasibility of utilizing the SD BIOLINE Onchocerciasis IgG4 rapid test in onchocerciasis surveillance in Senegal.
[So] Source:PLoS Negl Trop Dis;11(10):e0005884, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As effective onchocerciasis control efforts in Africa transition to elimination efforts, different diagnostic tools are required to support country programs. Senegal, with its long standing, successful control program, is transitioning to using the SD BIOLINE Onchocerciasis IgG4 (Ov16) rapid test over traditional skin snip microscopy. The aim of this study is to demonstrate the feasibility of integrating the Ov16 rapid test into onchocerciasis surveillance activities in Senegal, based on the following attributes of acceptability, usability, and cost. A cross-sectional study was conducted in 13 villages in southeastern Senegal in May 2016. Individuals 5 years and older were invited to participate in a demographic questionnaire, an Ov16 rapid test, a skin snip biopsy, and an acceptability interview. Rapid test technicians were interviewed and a costing analysis was conducted. Of 1,173 participants, 1,169 (99.7%) agreed to the rapid test while 383 (32.7%) agreed to skin snip microscopy. The sero-positivity rate of the rapid test among those tested was 2.6% with zero positives 10 years and younger. None of the 383 skin snips were positive for Ov microfilaria. Community members appreciated that the rapid test was performed quickly, was not painful, and provided reliable results. The total costs for this surveillance activity was $22,272.83, with a cost per test conducted at $3.14 for rapid test, $7.58 for skin snip microscopy, and $13.43 for shared costs. If no participants had refused skin snip microscopy, the total cost per method with shared costs would have been around $16 per person tested. In this area with low onchocerciasis sero-positivity, there was high acceptability and perceived value of the rapid test by community members and technicians. This study provides evidence of the feasibility of implementing the Ov16 rapid test in Senegal and may be informative to other country programs transitioning to Ov16 serologic tools.
[Mh] Termos MeSH primário: Anticorpos Anti-Helmínticos/sangue
Imunoglobulina G/sangue
Onchocerca volvulus/imunologia
Oncocercose/diagnóstico
Vigilância da População/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Criança
Pré-Escolar
Estudos Transversais
Estudos de Viabilidade
Feminino
Custos de Cuidados de Saúde
Seres Humanos
Masculino
Meia-Idade
Oncocercose/sangue
Oncocercose/economia
Oncocercose/epidemiologia
Aceitação pelo Paciente de Cuidados de Saúde
Senegal/epidemiologia
Testes Sorológicos/economia
Testes Sorológicos/métodos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Immunoglobulin G)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005884


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[PMID]:28934436
[Au] Autor:Norice-Tra CT; Ribeiro J; Bennuru S; Fay MP; Tyagi R; Mitreva M; Nutman TB
[Ad] Endereço:Laboratory of Parasitic Diseases.
[Ti] Título:Insights Into Onchocerca volvulus Population Biology Through Multilocus Immunophenotyping.
[So] Source:J Infect Dis;216(6):736-743, 2017 Sep 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have developed a serologically based immunophenotyping approach to study Onchocerca volvulus (Ov) population diversity. Using genomic sequence data and polymerase chain reaction-based genotyping, we identified nonsynonymous single-nucleotide polymorphisms (SNPs) in the genes of 16 major immunogenic Ov proteins: Ov-CHI-1/Ov-CHI-2, Ov16, Ov-FAR-1, Ov-CPI-1, Ov-B20, Ov-ASP-1, Ov-TMY-1, OvSOD1, OvGST1, Ov-CAL-1, M3/M4, Ov-RAL-1, Ov-RAL-2, Ov-ALT-1, Ov-FBA-1, and Ov-B8. We assessed the immunoreactivity of onchocerciasis patient sera (n = 152) from the Americas, West Africa, Central Africa, and East Africa against peptides derived from 10 of these proteins containing SNPs. Statistically significant variation in immunoreactivity among the regions was seen in SNP-containing peptides derived from 8 of 10 proteins tested: OVOC1192(1-15), OVOC9988(28-42), OVOC9225(320-334), OVOC7453(22-36), OVOC11517(14-28), OVOC3177(283-297), OVOC7911(594-608), and OVOC12628(174-188). Our data show that differences in immunoreactivity to variant antigenic peptides may be used to characterize Ov populations, thereby elucidating features of Ov population biology previously inaccessible because of the limited availability of parasite material.
[Mh] Termos MeSH primário: Genética Populacional
Proteínas de Helminto/genética
Imunofenotipagem
Onchocerca volvulus/genética
Oncocercose/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Anticorpos Anti-Helmínticos/sangue
Formação de Anticorpos
Antígenos de Helmintos/sangue
Criança
DNA de Protozoário/genética
Feminino
Técnicas de Genotipagem
Seres Humanos
Masculino
Meia-Idade
Onchocerca volvulus/isolamento & purificação
Oncocercose/imunologia
Polimorfismo de Nucleotídeo Único
Análise de Sequência de DNA
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (DNA, Protozoan); 0 (Helminth Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix394


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[PMID]:28922418
[Au] Autor:Lau CL; Sheridan S; Ryan S; Roineau M; Andreosso A; Fuimaono S; Tufa J; Graves PM
[Ad] Endereço:Department of Global Health, Research School of Population Health, The Australian National University, Canberra, Australia.
[Ti] Título:Detecting and confirming residual hotspots of lymphatic filariasis transmission in American Samoa 8 years after stopping mass drug administration.
[So] Source:PLoS Negl Trop Dis;11(9):e0005914, 2017 Sep.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Global Programme to Eliminate Lymphatic Filariasis (LF) aims to eliminate the disease as a public health problem by 2020 by conducting mass drug administration (MDA) and controlling morbidity. Once elimination targets have been reached, surveillance is critical for ensuring that programmatic gains are sustained, and challenges include timely identification of residual areas of transmission. WHO guidelines encourage cost-efficient surveillance, such as integration with other population-based surveys. In American Samoa, where LF is caused by Wuchereria bancrofti, and Aedes polynesiensis is the main vector, the LF elimination program has made significant progress. Seven rounds of MDA (albendazole and diethycarbamazine) were completed from 2000 to 2006, and Transmission Assessment Surveys were passed in 2010/2011 and 2015. However, a seroprevalence study using an adult serum bank collected in 2010 detected two potential residual foci of transmission, with Og4C3 antigen (Ag) prevalence of 30.8% and 15.6%. We conducted a follow up study in 2014 to verify if transmission was truly occurring by comparing seroprevalence between residents of suspected hotspots and residents of other villages. In adults from non-hotspot villages (N = 602), seroprevalence of Ag (ICT or Og4C3), Bm14 antibody (Ab) and Wb123 Ab were 1.2% (95% CI 0.6-2.6%), 9.6% (95% CI 7.5%-12.3%), and 10.5% (95% CI 7.6-14.3%), respectively. Comparatively, adult residents of Fagali'i (N = 38) had significantly higher seroprevalence of Ag (26.9%, 95% CI 17.3-39.4%), Bm14 Ab (43.4%, 95% CI 32.4-55.0%), and Wb123 Ab 55.2% (95% CI 39.6-69.8%). Adult residents of Ili'ili/Vaitogi/Futiga (N = 113) also had higher prevalence of Ag and Ab, but differences were not statistically significant. The presence of transmission was demonstrated by 1.1% Ag prevalence (95% CI 0.2% to 3.1%) in 283 children aged 7-13 years who lived in one of the suspected hotspots; and microfilaraemia in four individuals, all of whom lived in the suspected hotspots, including a 9 year old child. Our results provide field evidence that integrating LF surveillance with other surveys is effective and feasible for identifying potential hotspots, and conducting surveillance at worksites provides an efficient method of sampling large populations of adults.
[Mh] Termos MeSH primário: Filariose Linfática/epidemiologia
Filariose Linfática/transmissão
Filaricidas/administração & dosagem
Wuchereria bancrofti/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Aedes/imunologia
Albendazol/administração & dosagem
Albendazol/uso terapêutico
Samoa Americana/epidemiologia
Animais
Anticorpos Anti-Helmínticos/sangue
Antígenos de Helmintos/sangue
Criança
Pré-Escolar
Dietilcarbamazina/administração & dosagem
Dietilcarbamazina/uso terapêutico
Erradicação de Doenças
Esquema de Medicação
Filariose Linfática/imunologia
Filariose Linfática/parasitologia
Feminino
Filaricidas/uso terapêutico
Seguimentos
Seres Humanos
Masculino
Meia-Idade
Prevalência
Estudos Soroepidemiológicos
Wuchereria bancrofti/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Filaricides); F4216019LN (Albendazole); V867Q8X3ZD (Diethylcarbamazine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005914


  7 / 6526 MEDLINE  
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[PMID]:28819487
[Au] Autor:de Souza DK; Owusu IO; Otchere J; Adimazoya M; Frempong K; Ahorlu CS; Boakye DA; Wilson MD
[Ad] Endereço:Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Ghana.
[Ti] Título:An evaluation of Wb123 antibody elisa in individuals treated with ivermectin and albendazole, and implementation challenges in Africa.
[So] Source:Pan Afr Med J;27:65, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:The development of antibody testing for the diagnosis of lymphatic filariasis (LF) is intended to enhance the monitoring and evaluation activities of the Global Program for the Elimination of LF. This is due to the fact that antibody tests are expected to be the most sensitive at detecting exposure to LF compared to antigen that takes longer to develop. To this end a new antibody-based enzyme linked immunosorbent assay (ELISA) to antigen Wb123 has been developed and further designed into a point of care rapid diagnostic test, under evaluation. In pre-treatment surveys, individuals were tested for antigen using the immuno-chromatographic test (ICT) card, and night blood microfilariae, after which all positives were treated using Ivermectin and Albendazole. The Wb123 ELISA was tested in antigen positive individuals, three months after they were treated. Samples were also tested for ICT and night blood microfilariae. The results revealed a reduction in microfilariae and ICT prevalence after treatment. Antigen and antibody prevalence increased with age. However, there was no correlation with the antibody responses observed. The mean WB123 antibody titers were higher among ICT positives, but not significantly different from ICT negative persons. While the Wb123 is targeted for use in untreated populations, further evaluations and guidelines will be required to define its use in populations that have undergone treatment for the control of LF.
[Mh] Termos MeSH primário: Anti-Helmínticos/administração & dosagem
Anticorpos Anti-Helmínticos/sangue
Filariose Linfática/diagnóstico
Wuchereria bancrofti/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Fatores Etários
Idoso
Idoso de 80 Anos ou mais
Albendazol/administração & dosagem
Animais
Antígenos de Helmintos/sangue
Criança
Estudos Transversais
Filariose Linfática/tratamento farmacológico
Filariose Linfática/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Seres Humanos
Imunocromatografia/métodos
Ivermectina/administração & dosagem
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Antibodies, Helminth); 0 (Antigens, Helminth); 70288-86-7 (Ivermectin); F4216019LN (Albendazole)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.27.65.11004


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[PMID]:28759601
[Au] Autor:Farmer A; Beltran T; Choi YS
[Ad] Endereço:Infectious Disease Service, Department of Medicine, Womack Army Medical Center, Fort Bragg, North Carolina, United States of America.
[Ti] Título:Prevalence of Toxocara species infection in the U.S.: Results from the National Health and Nutrition Examination Survey, 2011-2014.
[So] Source:PLoS Negl Trop Dis;11(7):e0005818, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Toxocariasis is one of the most common neglected infections of poverty in the U.S. with a reported National Health and Nutrition Examination Survey (NHANES) III (1988-1994) seroprevalence of 13.9% based on enzyme immunoassay testing. We reviewed NHANES data from 2011-2014 to assess current levels. Sera collected from NHANES 2011-2014 participants six years and older were tested for exposure using rTc-CTL-1 antigen, a more sensitive and specific recombinant antigen for IgG antibodies for Toxocara spp. These results were subdivided into children (age 6-17) and adults (age ≥ 18) and then compared between various sociodemographic characteristics. Given prior associations of Toxocara exposure with atopic disease and lead exposure, we also reviewed laboratory values including complete blood counts and blood and urine lead levels. Data from 13,509 individuals with Toxocara antibody results were examined including 3337 children (15.2%) and 10172 adults (84.8%). Overall seroprevalence was 5.1%. In adults increased antibody positivity occurred with non-White ethnicity, male gender, less than college-level education and lower income. Among children, increased antibody positivity was solely related to a lack of health insurance. Additionally, seropositivity was associated with increased blood lead and eosinophil levels in adults and both blood and urine lead levels in children. Relative to NHANES III (1988-1994), current data suggest an overall decrease in Toxocara spp. seroprevalence from 13.9% to 5.1%, however this may be artificially lowered due to difference in testing methods used. Persistent disparities appear to be associated with at-risk populations such as minority ethnicity and low socioeconomic status.
[Mh] Termos MeSH primário: Anticorpos Anti-Helmínticos/sangue
Toxocaríase/sangue
Toxocaríase/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Contagem de Células Sanguíneas
Criança
Estudos Transversais
Feminino
Seres Humanos
Imunoglobulina G/sangue
Chumbo/sangue
Chumbo/urina
Modelos Lineares
Masculino
Meia-Idade
Análise Multivariada
Inquéritos Nutricionais
Pobreza
Fatores de Risco
Estudos Soroepidemiológicos
Toxocara
Estados Unidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Immunoglobulin G); 2P299V784P (Lead)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170827
[Lr] Data última revisão:
170827
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005818


  9 / 6526 MEDLINE  
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[PMID]:28750771
[Au] Autor:Costábile A; Marín M; Castillo E
[Ad] Endereço:Sección Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP 11400, Montevideo, Uruguay. Electronic address: aliciacostabile@gmail.com.
[Ti] Título:Spatio-temporal expression of Mesocestoides corti McVAL2 during strobilar development.
[So] Source:Exp Parasitol;181:30-39, 2017 Oct.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:VAL proteins belong to a diverse superfamily containing the CAP domain, with members described for various eukaryotic organisms, including parasites. They are implicated in diverse biological activities and, as secreted proteins, may be related in host - parasite interactions. For this reason they have been proposed as vaccine candidates against nematode infections. However, little is known about their function in cestodes. In M. corti, four partial cDNA sequences coding for members of the CAP superfamily were previously isolated. In this work we characterize the expression of McVAL2 in the larvae and segmented worms of M. corti, describing mRNA and protein localization using fluorescent microscopy. We also optimized real time PCR analysis for quantification of mRNA expression through the different stages of strobilar development. We show that McVAL2 is differentially located, depending on the developmental stage, and can be used as a molecular marker for the neuroendocrine system in the larvae. The dynamic and stage-specific expression patterns of McVAL2, combined with the large number of VAL proteins found in the genomes of parasitic flatworms, suggest varied roles for the VAL protein family in the biology of these parasites.
[Mh] Termos MeSH primário: Proteínas de Helminto/metabolismo
Mesocestoides/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anticorpos Anti-Helmínticos/metabolismo
DNA Complementar/química
DNA Complementar/genética
Equidae
Feminino
Expressão Gênica
Cabras
Proteínas de Helminto/química
Proteínas de Helminto/genética
Proteínas de Helminto/imunologia
Hibridização In Situ
Larva/genética
Larva/metabolismo
Masculino
Mesocestoides/crescimento & desenvolvimento
Mesocestoides/imunologia
Camundongos
RNA Mensageiro/análise
RNA Mensageiro/isolamento & purificação
Coelhos
Reação em Cadeia da Polimerase em Tempo Real
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (DNA, Complementary); 0 (Helminth Proteins); 0 (RNA, Messenger); 0 (Recombinant Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE


  10 / 6526 MEDLINE  
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[PMID]:28749939
[Au] Autor:Pedram B; Pasquetto V; Drame PM; Ji Y; Gonzalez-Moa MJ; Baldwin RK; Nutman TB; Biamonte MA
[Ad] Endereço:Drugs & Diagnostics for Tropical Diseases, San Diego, California, United States of America.
[Ti] Título:A novel rapid test for detecting antibody responses to Loa loa infections.
[So] Source:PLoS Negl Trop Dis;11(7):e0005741, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ivermectin-based mass drug administration (MDA) programs have achieved remarkable success towards the elimination of onchocerciasis and lymphatic filariasis. However, their full implementation has been hindered in Central Africa by the occurrence of ivermectin-related severe adverse events (SAEs) in a subset of individuals with high circulating levels of Loa loa microfilariae. Extending MDA to areas with coincident L. loa infection is problematic, and inexpensive point-of-care tests for L. loa are acutely needed. Herein, we present a lateral flow assay (LFA) to identify subjects with a serological response to Ll-SXP-1, a specific and validated marker of L. loa. The test was evaluated on serum samples from patients infected with L. loa (n = 109) and other helminths (n = 204), as well as on uninfected controls (n = 77). When read with the naked eye, the test was 94% sensitive for L. loa infection and was 100% specific when sera from healthy endemic and non-endemic controls or from those with S. stercoralis infections were used as the comparators. When sera of patients with O. volvulus, W. bancrofti, or M. perstans were used as the comparators, the specificity of the LFA was 82%, 87%, and 88%, respectively. A companion smartphone reader allowed measurement of the test line intensities and establishment of cutoff values. With a cutoff of 600 Units, the assay sensitivity decreased to 71%, but the specificity increased to 96% for O. volvulus, 100% for W. bancrofti, and 100% for M. perstans-infected individuals. The LFA may find applications in refining the current maps of L. loa prevalence, which are needed to eliminate onchocerciasis and lymphatic filariasis from the African continent.
[Mh] Termos MeSH primário: Anticorpos Anti-Helmínticos/sangue
Testes Diagnósticos de Rotina/métodos
Imunocromatografia/métodos
Loa/imunologia
Loíase/diagnóstico
Sistemas Automatizados de Assistência Junto ao Leito
[Mh] Termos MeSH secundário: África Central
Animais
Seres Humanos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005741



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