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[PMID]:29320815
[Au] Autor:Matin S; Shahbazi G; Namin ST; Moradpour R; Feizi F; Piri-Dogahe H
[Ad] Endereço:Department of Internal Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
[Ti] Título:Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran.
[So] Source:Korean J Parasitol;55(6):607-611, 2017 Dec.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.
[Mh] Termos MeSH primário: Aborto Habitual/etiologia
Aborto Espontâneo/etiologia
Anticorpos Antiprotozoários/sangue
Reação em Cadeia da Polimerase/métodos
Complicações Parasitárias na Gravidez/diagnóstico
Testes Sorológicos/métodos
Toxoplasma/imunologia
Toxoplasmose/diagnóstico
Toxoplasmose/parasitologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Biomarcadores/sangue
DNA de Protozoário/sangue
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Irã (Geográfico)/epidemiologia
Gravidez
Complicações Parasitárias na Gravidez/epidemiologia
Complicações Parasitárias na Gravidez/parasitologia
Toxoplasma/genética
Toxoplasmose/complicações
Toxoplasmose/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Biomarkers); 0 (DNA, Protozoan); 0 (Immunoglobulin G); 0 (Immunoglobulin M)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.6.607


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[PMID]:28453188
[Au] Autor:Moritz ED; Tonnetti L; Hewins ME; Berardi VP; Dodd RY; Stramer SL
[Ad] Endereço:Scientific Affairs Department, American Red Cross, Gaithersburg, Maryland.
[Ti] Título:Description of 15 DNA-positive and antibody-negative "window-period" blood donations identified during prospective screening for Babesia microti.
[So] Source:Transfusion;57(7):1781-1786, 2017 07.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Blood donation screening detecting only antibodies fails to identify donors in the earliest stage of infection, before a detectable immunologic response, that is, the "window period" (WP). We present data on WP donations identified during prospective screening for Babesia microti, a transfusion-transmissible parasite of increasing concern in the United States. STUDY DESIGN AND METHODS: Blood donations collected in Connecticut, Massachusetts, Minnesota, and Wisconsin were screened using polymerase chain reaction (PCR) and arrayed fluorescence immunoassay (AFIA) to detect B. microti DNA and antibodies, respectively. Parasite loads were estimated using quantitative PCR. Red blood cell (RBC) samples were inoculated into hamsters to assess infectivity. Donors screening reactive were indefinitely deferred, tested by supplemental methods, and followed to assess DNA and antibody clearance. Demographic data from WP donors (i.e., those screening PCR positive and AFIA negative) were compared to data from other positive donors. RESULTS: Of 220,479 donations screened from June 2012 to August 2016, a total of 700 were positive, of which 15 (2% of positive donations or 1 per 14,699 screened donations) were confirmed WP donations. The median estimated parasite load in WP donations was 350 parasites/mL, no different than AFIA-positive and PCR-positive donors. Parasite loads in RBC samples from WP units ranged from 14 to 11,022 parasites/mL; RBC samples from three of 10 (30%) WP donations infected hamsters. The mean age of WP donors was 48 years (range, 17-75 years); three (20%) were female. WP donor demographics did not differ significantly from demographics of other donors. CONCLUSIONS: We report one per 15,000 B. microti WP infections in blood donors in endemic areas, demonstrating the importance of nucleic acid testing to mitigate the risk of transfusion-transmitted babesiosis.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Babesia microti/isolamento & purificação
Doadores de Sangue
DNA de Protozoário/sangue
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Babesia microti/genética
Babesia microti/imunologia
Feminino
Imunofluorescência
Seres Humanos
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Estudos Prospectivos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (DNA, Protozoan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1111/trf.14103


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[PMID]:29309784
[Au] Autor:Bassi PB; de Araújo FF; Garcia GC; Vinícius da Silva M; Oliveira CJF; Bittar ER; de Souza Gomes M; Rodrigues do Amaral L; Costa E Silva MF; Nascentes GAN; Rodrigues Junior V; Martins-Filho OA; Araújo MSS; Bittar JFF
[Ad] Endereço:Universidade de Uberaba (UNIUBE), Medicina Veterinária, Mestrado em Sanidade e Produção Animal nos Trópicos - Avenida Nenê Sabino 1697/1698, 38055-500, Uberaba, MG, Brazil. Electronic address: vet.bassi@gmail.com.
[Ti] Título:Parasitological and immunological evaluation of cattle experimentally infected with Trypanosoma vivax.
[So] Source:Exp Parasitol;185:98-106, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Trypanosoma vivax infection causes relevant economical impact due to high morbidity and mortality leading to negative impact on local livestock. Despite parasitological and serological methods are used for the diagnosis of T. vivax infection, gaps regarding sensitivity and specificity of these methods still represent a challenge. The present study aimed to compare the kinetics of parasitological and serological parameters in cattle experimentally infected with T. vivax along with immunophenotypic analysis of whole blood leukocytes. Based on the parasitemia profile the analysis were performed in three distinct periods, referred as pre-patent, patent and post-treatment. Distinct kinetics of anti-T. vivax IgM and IgG were observed during the pre-patent, patent and post-treatment periods. Increased levels of WC1 γδ T-cells were observed throughout the infection with strong correlations with other biomarkers observed during post-treatment period. Our findings demonstrated that there is a important participation of Monocytes:CD14 ; NK-cells:CD335 and WC1 γδ T-cells that coincide with the peak of parasitemia and also with the adaptive immunity, specially CD4 T-cells in T. vivax infection. The knowledge of the immune response is important not only for understanding the biology of the parasite in the host, but for the design of new treatment strategies for trypanosome infections.
[Mh] Termos MeSH primário: Doenças dos Bovinos/imunologia
Parasitemia/veterinária
Trypanosoma vivax/imunologia
Tripanossomíase Africana/veterinária
[Mh] Termos MeSH secundário: Imunidade Adaptativa
Animais
Anticorpos Antiprotozoários/sangue
Biomarcadores/análise
Bovinos
Doenças dos Bovinos/tratamento farmacológico
Doenças dos Bovinos/parasitologia
Diminazena/uso terapêutico
Técnica Indireta de Fluorescência para Anticorpo/veterinária
Imunidade Inata
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Imunofenotipagem/veterinária
Leucócitos/classificação
Leucócitos/imunologia
Masculino
Parasitemia/tratamento farmacológico
Parasitemia/imunologia
Parasitemia/parasitologia
Distribuição Aleatória
Tripanossomicidas/uso terapêutico
Tripanossomíase Africana/tratamento farmacológico
Tripanossomíase Africana/imunologia
Tripanossomíase Africana/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Biomarkers); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (Trypanocidal Agents); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


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[PMID]:29309783
[Au] Autor:Picchio MS; Sánchez VR; Arcon N; Soto AS; Perrone Sibilia M; Aldirico MLA; Urrutia M; Moretta R; Fenoy IM; Goldman A; Martin V
[Ad] Endereço:Laboratorio de Inmunología, Vacunas y Alergia, CESyMA, Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Campus Migueletes, Martín de Irigoyen 3100 C.P., 1650 San Martín, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290
[Ti] Título:Vaccine potential of antigen cocktails composed of recombinant Toxoplasma gondii TgPI-1, ROP2 and GRA4 proteins against chronic toxoplasmosis in C3H mice.
[So] Source:Exp Parasitol;185:62-70, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The development of an effective and safe vaccine to prevent Toxoplasma gondii infection is an important aim due to the great clinical and economic impact of this parasitosis. We have previously demonstrated that immunization with the serine protease inhibitor-1 (TgPI-1) confers partial protection to C3H/HeN and C57BL/6 mice. In order to improve the level of protection, in this work, we combined this novel antigen with ROP2 and/or GRA4 recombinant proteins (rTgPI-1+rROP2, rTgPI-1+rGRA4, rTgPI-1+rROP2+rGRA4) to explore the best combination against chronic toxoplasmosis in C3H/HeN mice. All tested vaccine formulations, administered following a homologous prime-boost protocol that combines intradermal and intranasal routes, conferred partial protection as measured by the reduction of brain cyst burden following oral challenge with tissue cysts of Me49 T. gondii strain. The highest level of protection was achieved by the mixture of rTgPI-1 and rROP2 proteins with an average parasite burden reduction of 50% compared to the unvaccinated control group. The vaccine-induced protective effect was related to the elicitation of systemic cellular and humoral immune responses that included antigen-specific spleen cell proliferation, the release of Th1/Th2 cytokines, and the generation of antigen-specific antibodies in serum. Additionally, mucosal immune responses were also induced, characterized by secretion of antigen-specific IgA antibodies in intestinal lavages and specific mesenteric lymph node cell proliferation. Our results demonstrate that rTgPI-1+rROP2 antigens seem a promising mixture to be combined with other immunogenic proteins in a multiantigenic vaccine formulation against toxoplasmosis.
[Mh] Termos MeSH primário: Antígenos de Protozoários/imunologia
Vacinas Protozoárias/normas
Toxoplasma/imunologia
Toxoplasmose Animal/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/sangue
Linhagem Celular
Doença Crônica
Citocinas/metabolismo
Feminino
Fibroblastos/parasitologia
Prepúcio do Pênis/citologia
Seres Humanos
Imunoglobulina A Secretora/análise
Imunoglobulina G/sangue
Mucosa Intestinal/imunologia
Masculino
Proteínas de Membrana/imunologia
Camundongos
Camundongos Endogâmicos C3H
Proteínas de Protozoários/imunologia
Baço/citologia
Baço/imunologia
Vacinas Sintéticas/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Cytokines); 0 (GRA4 protein, Toxoplasma gondii); 0 (Immunoglobulin A, Secretory); 0 (Immunoglobulin G); 0 (Membrane Proteins); 0 (Protozoan Proteins); 0 (Protozoan Vaccines); 0 (ROP 2 protein, Toxoplasma gondii); 0 (TgPI protein, Toxoplasma gondii); 0 (Vaccines, Synthetic)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


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Costa, Sandra Cecília Botelho
Texto completo
[PMID]:29315305
[Au] Autor:Martins MFDS; Pereira MB; Ferreira JJG; França AO; Cominetti MC; Ferreira EC; Dorval MEMC; Rossi CL; Mazon SB; de Almeida EA; Costa SCB; Marcon GEB
[Ad] Endereço:Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
[Ti] Título:Serological and molecular inquiry of Chagas disease in an Afro-descendant settlement in Mato Grosso do Sul State, Brazil.
[So] Source:PLoS One;13(1):e0189448, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Furnas do Dionísio is a Brazilian Afro-descendant settlement in the city of Jaraguari, 21.4 miles from Campo Grande, Mato Grosso do Sul, Brazil. Approximately 96 families live in this quilombola (Maroon) settlement, also known in Brazil as a remnant community of descendants of African slaves. Recent studies found 20% of households were infested by triatomines, 18% of insects captured in the community were infected by Trypanosoma cruzi, and 22.7% of dogs presented T. cruzi antibodies. The low prevalence of Chagas disease observed in humans in Mato Grosso do Sul State is attributed to its arrival via colonist migration and subsequent transplacental transmission. In order to gain a better understanding of the T. cruzi cycle in residents of the study community, serological and molecular tests were carried out to diagnose Chagas disease. In the present study, 175 residents between 2 and 80 years old were included. A total of 175 participants were interviewed and 170 provided blood samples, which were tested for T. cruzi antibodies with serological tests. Molecular diagnosis was performed in 167 participants by PCR (KDNA) and NPCR (satellite DNA) tests. One of the 170 samples tested positive for all serological tests performed. The overall frequency of Chagas disease in the community was low (0.6%). Interview responses revealed that 66.3% knew of triatomine insects and 65.7% reported having had no contact with them. Physical improvements to residences, together with vector surveillance and control by the State and municipal governments and local ecological conservation contribute to the low frequency of the Chagas disease in this quilombola community.
[Mh] Termos MeSH primário: Grupo com Ancestrais do Continente Africano
Doença de Chagas/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Anticorpos Antiprotozoários/sangue
Brasil/epidemiologia
Doença de Chagas/sangue
Doença de Chagas/genética
Criança
Feminino
Seres Humanos
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Estudos Soroepidemiológicos
Trypanosoma cruzi/genética
Trypanosoma cruzi/imunologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189448


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[PMID]:29267280
[Au] Autor:Rial MS; Scalise ML; Arrúa EC; Esteva MI; Salomon CJ; Fichera LE
[Ad] Endereço:Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben", ANLIS "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina.
[Ti] Título:Elucidating the impact of low doses of nano-formulated benznidazole in acute experimental Chagas disease.
[So] Source:PLoS Negl Trop Dis;11(12):e0006119, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Chagas disease is a neglected parasitic infection caused by the protozoan Trypanosoma cruzi (T. cruzi) that affects more than 6 million people, mainly in Latin America. Benznidazole is still the drug of choice in many countries to treat it in spite of its dosage regimen and adverse side effects such as such as allergic dermatitis, peripheral neuropathy and anorexia. Thus, novel, safer, and more efficacious treatments for such neglected infection are urgently required. METHODOLOGY: In this study, the efficacy of orally administered low doses of benznidazole (BNZ) nanoparticles was evaluated during the acute phase in mice infected with T. cruzi Nicaragua (TcN) that were immunosuppressed during the chronic stage of the disease. Moreover, the production of T. cruzi-specific antibodies, cardiac tissue inflammation and reactive oxygen species generation by Vero cells treated with both BNZ nanoparticles (BNZ-nps) and raw BNZ (R-BNZ) were also evaluated. PRINCIPAL FINDINGS: T. cruzi infected mice treated with 10, 25 or 50 mg/kg/day of BNZ-nps survived until euthanasia (92 days post infection (dpi)), while only 15% of infected untreated mice survived until the end of the experiment. PCR analysis of blood samples taken after induction of immunosuppression showed that a dosage of 25 mg/kg/day rendered 40% of the mice PCR-negative. The histological analysis of heart tissue showed a significant decrease in inflammation after treatments with 25 and 50 mg/kg/day, while a similar inflammatory damage was observed in both infected mice treated with R-BNZ (50 mg/kg/day) and untreated mice. In addition, only BNZ-nps treated mice led to lower levels of T. cruzi-specific antibodies to 50-100%. Finally, mammalian Vero cells treated with BNZ-nps or R-BNZ lead to a significant increase in ROS production. CONCLUSIONS: Based on these findings, this research highlights the in-vitro/in-vivo efficacy of nanoformulated BNZ against T. cruzi acute infections in immunosuppressed and non-immunosuppressed mice and provides further evidence for the optimization of dosage regimens to treat Chagas disease.
[Mh] Termos MeSH primário: Doença de Chagas/tratamento farmacológico
Nanopartículas/uso terapêutico
Nitroimidazóis/uso terapêutico
Tripanossomicidas/uso terapêutico
Trypanosoma cruzi/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/sangue
Anticorpos Antiprotozoários/imunologia
Linhagem Celular
Cercopithecus aethiops
Doença de Chagas/parasitologia
DNA de Protozoário/sangue
DNA de Protozoário/genética
Modelos Animais de Doenças
Portadores de Fármacos/uso terapêutico
Feminino
Coração/parasitologia
Inflamação/parasitologia
Camundongos
Camundongos Endogâmicos C3H
Espécies Reativas de Oxigênio/metabolismo
Trypanosoma cruzi/imunologia
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (DNA, Protozoan); 0 (Drug Carriers); 0 (Nitroimidazoles); 0 (Reactive Oxygen Species); 0 (Trypanocidal Agents); YC42NRJ1ZD (benzonidazole)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006119


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[PMID]:29281708
[Au] Autor:Scaria PV; Chen B; Rowe CG; Jones DS; Barnafo E; Fischer ER; Anderson C; MacDonald NJ; Lambert L; Rausch KM; Narum DL; Duffy PE
[Ad] Endereço:Laboratory of Malaria Immunology and Vaccinology, NIAID, National Institutes of Health, Rockville, Maryland, United States of America.
[Ti] Título:Protein-protein conjugate nanoparticles for malaria antigen delivery and enhanced immunogenicity.
[So] Source:PLoS One;12(12):e0190312, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemical conjugation of polysaccharide to carrier proteins has been a successful strategy to generate potent vaccines against bacterial pathogens. We developed a similar approach for poorly immunogenic malaria protein antigens. Our lead candidates in clinical trials are the malaria transmission blocking vaccine antigens, Pfs25 and Pfs230D1, individually conjugated to the carrier protein Exoprotein A (EPA) through thioether chemistry. These conjugates form nanoparticles that show enhanced immunogenicity compared to unconjugated antigens. In this study, we examined the broad applicability of this technology as a vaccine development platform, by comparing the immunogenicity of conjugates prepared by four different chemistries using different malaria antigens (PfCSP, Pfs25 and Pfs230D1), and carriers such as EPA, TT and CRM197. Several conjugates were synthesized using thioether, amide, ADH and glutaraldehyde chemistries, characterized for average molecular weight and molecular weight distribution, and evaluated in mice for humoral immunogenicity. Conjugates made with the different chemistries, or with different carriers, showed no significant difference in immunogenicity towards the conjugated antigens. Since particle size can influence immunogenicity, we tested conjugates with different average size in the range of 16-73 nm diameter, and observed greater immunogenicity of smaller particles, with significant differences between 16 and 73 nm particles. These results demonstrate the multiple options with respect to carriers and chemistries that are available for protein-protein conjugate vaccine development.
[Mh] Termos MeSH primário: Antígenos de Protozoários/administração & dosagem
Nanopartículas
Proteínas/química
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/biossíntese
Antígenos de Protozoários/imunologia
Camundongos
Tamanho da Partícula
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190312


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[PMID]:29246832
[Au] Autor:Marques T; Silva GC; Henrique Paiva PM; Nascentes GAN; Ramirez LE; Norris K; Meira WSF
[Ad] Endereço:Programa de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
[Ti] Título:Use of Tc-rCRP as a target for lytic antibody titration after experimental Trypanosoma cruzi infection.
[So] Source:Exp Parasitol;184:103-108, 2018 Jan.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Experimental Chagas disease has been used as a model to identify several host/parasite interaction factors involved in immune responses to Trypanosoma cruzi infection. One of the factors inherent to this parasite is the complement regulatory protein (Tc-CRP), a major epitope that induces production of lytic antibodies during T. cruzi infections. Previous studies have evaluated the function of Tc-CRP as an antigenic marker via ELISAs, which demonstrated high sensitivity and specificity when compared to other methods. Therefore, this study aimed to assess and compare the levels of lytic antibodies induced by this protein following experimental infection using different T. cruzi strains. Our results demonstrated that infections induced by strains isolated from vectors resulted in subpatent parasitaemia and low reactivity, as assessed by Tc-rCRP ELISAs. On the other hand, mice inoculated with T. cruzi strains isolated from patients developed patent parasitaemia, and presented elevated lytic antibodies titres, as measured by Tc-rCRP ELISA. In addition, comparison between different mouse lineages demonstrated that Balb/c mice were more reactive than C57BL/6 mice in almost all types of infections, except those infected by the AQ-4 strain. Parasites from the Hel strain generated the greatest lytic antibody response in all evaluated models. Therefore, application of sensitive techniques for monitoring immune responses would enable us to establish growth curves for lytic antibodies during the course of the infection, and allow us to discriminate between T. cruzi strains that originate from different hosts.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/análise
Antígenos de Protozoários/imunologia
Doença de Chagas/imunologia
Proteínas de Protozoários/imunologia
Trypanosoma cruzi/imunologia
[Mh] Termos MeSH secundário: Animais
Biomarcadores/análise
Ensaio de Imunoadsorção Enzimática
Epitopos/imunologia
Interações Hospedeiro-Parasita/imunologia
Seres Humanos
Insetos Vetores/parasitologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Parasitemia/imunologia
Parasitemia/parasitologia
Sensibilidade e Especificidade
Triatominae/parasitologia
Trypanosoma cruzi/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Biomarkers); 0 (Epitopes); 0 (Protozoan Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


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[PMID]:28468666
[Au] Autor:Silva JCR; Ferreira F; Dias RA; Ajzenberg D; Marvulo MFV; Magalhães FJR; Filho CDFL; Oliveira S; Soares HS; Feitosa TF; Aizawa J; Alves LC; Mota RA; Dubey JP; Gennari SM; Pena HFJ
[Ad] Endereço:Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.
[Ti] Título:Cat-rodent Toxoplasma gondii Type II-variant circulation and limited genetic diversity on the Island of Fernando de Noronha, Brazil.
[So] Source:Parasit Vectors;10(1):220, 2017 May 03.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In Brazil, studies on animals and humans in mainland areas have shown that most strains of Toxoplasma gondii are pathogenic to mice and exhibit great genetic variability. RESULTS: In this study, using a set of 11 PCR-RFLP and 15 microsatellite markers, we isolated and genetically characterised T. gondii strains from one cat and three rats on Fernando de Noronha Island. The cat had antibodies to T. gondii, which were revealed using a modified agglutination test (MAT, cut-off 1:25) and the seroprevalence among the 46 rodents was 15.2%. Viable T. gondii was isolated from one cat (TgCatBrFN1), two brown rats (TgRatnoBrFN1 and TgRatnoBrFN2) and one black rat (TgRatraBrFN1). Unlike the strains from mainland Brazil, these isolates were not pathogenic to outbred mice. The genotypes of these strains were compared with strains previously isolated on the island and in mainland Brazil. The analysis based on microsatellite data showed a limited genetic diversity of T. gondii on Fernando de Noronha Island with the majority of strains clustered into the following three groups: type II, III, and Caribbean 1. CONCLUSIONS: There was little variation among strains within the same group, suggesting that the majority of strains circulating on Fernando de Noronha are derived from only a few strains that were recently introduced to the island, likely from imported cats. Except for the strain belonging to the Caribbean 1 group that originates from northeast Brazil, there was little evidence that strains from the other groups were introduced to Fernando de Noronha via mainland Brazil.
[Mh] Termos MeSH primário: Doenças do Gato/parasitologia
Variação Genética
Doenças dos Roedores/parasitologia
Toxoplasma/genética
Toxoplasma/isolamento & purificação
Toxoplasmose Animal/parasitologia
[Mh] Termos MeSH secundário: Testes de Aglutinação
Animais
Animais Selvagens
Anticorpos Antiprotozoários/sangue
Brasil/epidemiologia
Gatos/parasitologia
Genótipo
Seres Humanos
Ilhas
Camundongos
Repetições de Microssatélites
Reação em Cadeia da Polimerase/veterinária
Polimorfismo de Fragmento de Restrição
Ratos
Roedores/parasitologia
Estudos Soroepidemiológicos
Toxoplasma/imunologia
Toxoplasma/patogenicidade
Toxoplasmose Animal/epidemiologia
Toxoplasmose Animal/imunologia
Toxoplasmose Animal/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2150-4


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[PMID]:29228997
[Au] Autor:Santoro A; Tagel M; Must K; Laine M; Lassen B; Jokelainen P
[Ad] Endereço:Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy.
[Ti] Título:Toxoplasma gondii seroprevalence in breeding pigs in Estonia.
[So] Source:Acta Vet Scand;59(1):82, 2017 Dec 11.
[Is] ISSN:1751-0147
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Toxoplasma gondii is a widespread occurring parasite infecting warm-blooded animals, including pigs and humans. The aims of this study were to estimate the prevalence of anti-T. gondii antibodies and to evaluate risk factors for T. gondii seropositivity in breeding pigs raised in Estonia. Sera from 382 pigs were tested with a commercial direct agglutination test, using a cut-off titer of 40 for seropositivity, for the presence of anti-T. gondii immunoglobulin G antibodies. RESULTS: Twenty-two (5.8%) of the 382 pigs tested seropositive for T. gondii, and 6 of the 14 herds had at least one seropositive pig. The proportion of seropositive pigs within the herds ranged between 0 and 43%. Gender appeared as a significant factor, with sows having 5.6 times higher odds to be seropositive to T. gondii than boars. Seroprevalence did not increase with age. CONCLUSIONS: Anti-T. gondii antibodies were present in a substantial proportion of breeding pig herds in Estonia. On the other hand, the presence of herds without seropositive pigs illustrates that porcine T. gondii infections can be avoided even in a country where the parasite is endemic and common in several other host species.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Doenças dos Suínos/epidemiologia
Toxoplasmose Animal/epidemiologia
[Mh] Termos MeSH secundário: Animais
Estônia/epidemiologia
Imunoglobulina G/sangue
Estudos Soroepidemiológicos
Suínos
Toxoplasma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Immunoglobulin G)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1186/s13028-017-0349-1



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