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[PMID]:28457901
[Au] Autor:Haghighat S; Siadat SD; Rezayat Sorkhabadi SM; Akhavan Sepahi A; Sadat SM; Yazdi MH; Mahdavi M
[Ad] Endereço:Department of Microbiology, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran. Electronic address: Haghighat.s@iaups.ac.ir.
[Ti] Título:Recombinant PBP2a as a vaccine candidate against methicillin-resistant Staphylococcus aureus: Immunogenicity and protectivity.
[So] Source:Microb Pathog;108:32-39, 2017 Jul.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Methicillin-resistant Staphylococcus aureus infections are focal and development of an effective vaccine can help to control this infection. Here, recombinant PBP2a was studied in mouse model. Following the preparation of recombinant PBP2a, Balb/c mice were injected subcutaneously with 20 µg of r-PBP2a formulated in Freund's adjuvant three times with three weeks intervals with proper control group. Total and specific isotype antibodies were evaluated on sera by ELISA. Opsonophagocytic activity was also investigated on the sera samples. Intraperitonealchallenge with a sub-lethal dose of MRSA (5 × 10 CFU) was done in experimental mice. Following that, the number of bacteria from kidneys of experimental mice were determined. Survival rate was recorded for 60 days. Significant increase of antibody with high level of IgG1, IgG2a and IgG2b isotypes was demonstrated in vaccinated mice versus the control group (P < 0.005). The bacterial load in the kidneys from immunized mice was 1000 times less thancontrol group (PBS) and opsonophagocytic activity of immunized mice sera significantly increased (P < 0.0001). Finally the life span of immunized mice after bacterial challenge was extended versus control mice. These results may indicate the capacity of PBP2a as a candidate vaccine to control the MRSA infections.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Proteínas de Bactérias/imunologia
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/imunologia
Proteínas de Ligação às Penicilinas/genética
Proteínas de Ligação às Penicilinas/imunologia
Infecções Estafilocócicas/imunologia
Vacinas Antiestafilocócicas/genética
Vacinas Antiestafilocócicas/imunologia
Vacinas Sintéticas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/genética
Antígenos de Bactérias/imunologia
Carga Bacteriana
Clonagem Molecular
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Feminino
Imunidade Humoral/imunologia
Imunoglobulina G/sangue
Isotipos de Imunoglobulinas/imunologia
Rim/efeitos dos fármacos
Rim/microbiologia
Camundongos
Camundongos Endogâmicos BALB C
Alinhamento de Sequência
Análise de Sequência
Infecções Estafilocócicas/microbiologia
Infecções Estafilocócicas/prevenção & controle
Taxa de Sobrevida
Vacinação
Vacinas Sintéticas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (Immunoglobulin G); 0 (Immunoglobulin Isotypes); 0 (Penicillin-Binding Proteins); 0 (Staphylococcal Vaccines); 0 (Vaccines, Synthetic); 0 (mecA protein, Staphylococcus aureus)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  2 / 3863 MEDLINE  
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Avelino, Mariza Martins
Amaral, Waldemar Naves do
Castro, Ana Maria de
Texto completo SciELO Brasil
[PMID]:28902298
[Au] Autor:Rezende HHA; Storchilo HR; Lima JAS; Gomes AR; Gomes TC; Souza JY; Avelino MM; Amaral WND; Vinaud MC; Castro AM
[Ad] Endereço:Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Laboratório de Estudos da Relação Parasito-Hospedeiro, Goiânia, Goiás, Brazil.
[Ti] Título:Nursing infant with acquired toxoplasmosis in the first months of life - a case report.
[So] Source:Rev Inst Med Trop Sao Paulo;59:e63, 2017 Aug 24.
[Is] ISSN:1678-9946
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Toxoplasmosis is caused by Toxoplasma gondii and the probability of this infection occurring in the first months of life is usually low because its transmission is related to eating habits. A 6-month-old nursing infant was diagnosed with acute toxoplasmosis, which was identified through anti- T. gondii IgA, IgM and low-avidity IgG serologic assays, polymerase chain reaction (PCR) and mouse bioassay test although its mother was seronegative. This serological divergence between mother and child led us to interview the mother regarding epidemiological factors. During this interview, she reported that she had given her 2-month-old baby a piece of undercooked beef to suck on. After some time, the baby presented fever and cervical lymphadenitis. This report emphasizes the importance of serological surveys of toxoplasmosis in nursing infants presenting with fever and lymphadenitis, in view of the possible acquisition of toxoplasmosis in the first months of life.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Contaminação de Alimentos
Produtos da Carne/parasitologia
Toxoplasma/imunologia
Toxoplasmose/diagnóstico
Toxoplasmose/transmissão
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Isotipos de Imunoglobulinas/sangue
Lactente
Masculino
Camundongos
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:CASE REPORTS
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Immunoglobulin Isotypes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE


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[PMID]:28813656
[Au] Autor:Bournazos S; Ravetch JV
[Ad] Endereço:Laboratory of Molecular Genetics and Immunology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
[Ti] Título:Fcγ Receptor Function and the Design of Vaccination Strategies.
[So] Source:Immunity;47(2):224-233, 2017 Aug 15.
[Is] ISSN:1097-4180
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Through specific interactions with distinct types of Fcγ receptors (FcγRs), the Fc domain of immunoglobulin G (IgG) mediates a wide spectrum of immunological functions that influence both innate and adaptive responses. Recent studies indicate that IgG Fc-FcγR interactions are dynamically regulated during an immune response through the control of the Fc-associated glycan structure and Ig subclass composition on the one hand and selective FcγR expression on immune cells on the other, which together determine the capacity of IgG to interact in a cell-type-specific manner with specific members of the FcγR family. Here, we present a framework that synthesizes the current understanding of the contribution of FcγR pathways to the induction and regulation of antibody and T cell responses. Within this context, we discuss vaccination strategies to elicit broad and potent immune responses based on the immunomodulatory properties of Fc-FcγR interactions.
[Mh] Termos MeSH primário: Fragmentos Fc das Imunoglobulinas/metabolismo
Isotipos de Imunoglobulinas/metabolismo
Receptores de IgG/metabolismo
Linfócitos T Reguladores/imunologia
Vacinas/imunologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Isotipos de Imunoglobulinas/imunologia
Imunomodulação
Receptores de IgG/imunologia
Transdução de Sinais
Vacinação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Immunoglobulin Fc Fragments); 0 (Immunoglobulin Isotypes); 0 (Receptors, IgG); 0 (Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE


  4 / 3863 MEDLINE  
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[PMID]:28760881
[Au] Autor:Iacoangeli A; Lui A; Haines A; Ohta Y; Flajnik M; Hsu E
[Ad] Endereço:Tisch Multiple Sclerosis Research Center of New York, New York, NY 10019.
[Ti] Título:Evidence for Ig Light Chain Isotype Exclusion in Shark B Lymphocytes Suggests Ordered Mechanisms.
[So] Source:J Immunol;199(5):1875-1885, 2017 Sep 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Unlike most vertebrates, the shark IgL gene organization precludes secondary rearrangements that delete self-reactive VJ rearranged genes. Nurse sharks express four L chain isotypes, κ, λ, σ, and σ-2, encoded by 35 functional minigenes or clusters. The sequence of gene activation/expression and receptor editing of these isotypes have not been studied. We therefore investigated the extent of isotypic exclusion in separated B cell subpopulations. Surface Ig (sIg)κ-expressing cells, isolated with mAb LK14 that recognizes Cκ, carry predominantly nonproductive rearrangements of other L chain isotypes. Conversely, after depletion with LK14, sIgM cells contained largely nonproductive κ and enrichment for in-frame VJ of the others. Because some isotypic inclusion was observed at the mRNA level, expression in the BCR was examined. Functional λ mRNA was obtained, as expected, from the LK14-depleted population, but was also in sIgκ splenocytes. Whereas λ somatic mutants from the depleted sample displayed evidence of positive selection, the λ genes in sIgκ cells accumulated bystander mutations indicating a failure to express their products at the cell surface in association with the BCR H chain. In conclusion, a shark B cell expresses one L chain isotype at the surface and other isotypes as nonproductive VJ, sterile transcripts, or in-frame VJ whose products may not associate with the H chain. Based on the mRNA content found in the B cell subpopulations, an order of L chain gene activation is suggested as: σ-2 followed by κ, then σ and λ.
[Mh] Termos MeSH primário: Subpopulações de Linfócitos B/fisiologia
Linfócitos B/fisiologia
Proteínas de Peixes/genética
Rearranjo Gênico de Cadeia Leve de Linfócito B
Isotipos de Imunoglobulinas/genética
Cadeias Leves de Imunoglobulina/genética
Receptores de Antígenos de Linfócitos B/genética
Tubarões/imunologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Feminino
Regulação da Expressão Gênica
Loci Gênicos
Estruturas Genéticas
Switching de Imunoglobulina
Masculino
RNA Mensageiro
Vertebrados
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Immunoglobulin Isotypes); 0 (Immunoglobulin Light Chains); 0 (RNA, Messenger); 0 (Receptors, Antigen, B-Cell)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1700762


  5 / 3863 MEDLINE  
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[PMID]:28739769
[Au] Autor:Tang K; Sui LL; Xu G; Zhang T; Liu Q; Liu XF
[Ad] Endereço:Department of Hepatobiliary Surgery, Affiliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai, P.R. China.
[Ti] Título:Effects of Different Palliative Jaundice Reducing Methods on Immunologic Functions in Patients with Advanced Malignant Obstructive Jaundice.
[So] Source:Anticancer Res;37(8):4665-4670, 2017 08.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: This study aimed to investigate the effects of three treatment methods on the immunological function of patients with advanced malignant obstructive jaundice (MOJ). PATIENTS AND METHODS: Patients with advanced MOJ were randomly divided into three groups according to biliary drainage methods. Detection of levels of multi-indices were investigated in different time periods. RESULTS: After drainage, the levels of complement 3 (C3) and complement 4 (C4) were increased. Forteen days post-operation, the levels of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) in the group undergoing palliative surgery decreased significantly compared to those in both percutaneous transhepatic cholangio drainage (PTCD) and endoscopic retrograde biliary drainage (ERBD) groups. The level of serum endotoxin in the group undergoing palliative surgery decreased gradually. CONCLUSION: Palliative surgery for reducing jaundice is superior to PTCD and ERBD in improving immune function of patients with MOJ.
[Mh] Termos MeSH primário: Imunidade
Icterícia Obstrutiva/etiologia
Icterícia Obstrutiva/terapia
Neoplasias/complicações
Cuidados Paliativos
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Bilirrubina/sangue
Complemento C3/imunologia
Complemento C4/imunologia
Drenagem/métodos
Endotoxinas/sangue
Feminino
Seres Humanos
Isotipos de Imunoglobulinas/sangue
Isotipos de Imunoglobulinas/imunologia
Icterícia Obstrutiva/diagnóstico
Masculino
Meia-Idade
Imagem Multimodal
Neoplasias/diagnóstico
Cuidados Paliativos/métodos
Estudos Retrospectivos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complement C3); 0 (Complement C4); 0 (Endotoxins); 0 (Immunoglobulin Isotypes); RFM9X3LJ49 (Bilirubin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  6 / 3863 MEDLINE  
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[PMID]:28686710
[Au] Autor:Silva SL; Albuquerque A; Amaral AJ; Li QZ; Mota C; Cheynier R; Victorino RMM; Pereira-Santos MC; Sousa AE
[Ad] Endereço:Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa. Lisboa, Portugal.
[Ti] Título:Autoimmunity and allergy control in adults submitted to complete thymectomy early in infancy.
[So] Source:PLoS One;12(7):e0180385, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The contribution of the decline in thymic activity for the emergence of autoimmunity is still debatable. Immune-competent adults submitted to complete thymectomy early in life provide a unique model to address this question. We applied here strict criteria to identify adults lacking thymic activity based on sjTREC levels, to exclude thymic rebound and/or ectopic thymuses. In agreement, they featured severe naïve CD4 T-cell depletion and contraction of T-cell receptor diversity. Notwithstanding this, there was neither increased incidence of autoimmune disease in comparison with age-matched controls nor significant changes in their IgG/IgA/IgM/IgE autoreactivity profiles, as assessed through extensive arrays. We reasoned that the observed relative preservation of the regulatory T-cell compartment, including maintenance of naïve regulatory CD4 T-cells, may contribute to limit the emergence of autoimmunity upon thymectomy. Our findings have implications in other clinical settings with impaired thymic activity, and are particularly relevant to studies of autoimmunity in ageing.
[Mh] Termos MeSH primário: Envelhecimento/imunologia
Imunocompetência
Isotipos de Imunoglobulinas/biossíntese
Linfócitos T Reguladores/imunologia
Timectomia/reabilitação
Timo/cirurgia
[Mh] Termos MeSH secundário: Adulto
Doenças Autoimunes/prevenção & controle
Contagem de Linfócito CD4
Linfócitos T CD4-Positivos/citologia
Linfócitos T CD4-Positivos/imunologia
Feminino
Expressão Gênica
Seres Humanos
Hipersensibilidade/prevenção & controle
Isotipos de Imunoglobulinas/genética
Lactente
Estudos Longitudinais
Masculino
Receptores de Antígenos de Linfócitos T/deficiência
Receptores de Antígenos de Linfócitos T/genética
Receptores de Antígenos de Linfócitos T/imunologia
Linfócitos T Reguladores/citologia
Timo/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin Isotypes); 0 (Receptors, Antigen, T-Cell)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180385


  7 / 3863 MEDLINE  
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[PMID]:28628107
[Au] Autor:Jonsson S; Sveinbjornsson G; de Lapuente Portilla AL; Swaminathan B; Plomp R; Dekkers G; Ajore R; Ali M; Bentlage AEH; Elmér E; Eyjolfsson GI; Gudjonsson SA; Gullberg U; Gylfason A; Halldorsson BV; Hansson M; Holm H; Johansson Å; Johnsson E; Jonasdottir A; Ludviksson BR; Oddsson A; Olafsson I; Olafsson S; Sigurdardottir O; Sigurdsson A; Stefansdottir L; Masson G; Sulem P; Wuhrer M; Wihlborg AK; Thorleifsson G; Gudbjartsson DF; Thorsteinsdottir U; Vidarsson G; Jonsdottir I; Nilsson B; Stefansson K
[Ad] Endereço:deCODE Genetics/Amgen, Inc., Reykjavik, Iceland.
[Ti] Título:Identification of sequence variants influencing immunoglobulin levels.
[So] Source:Nat Genet;49(8):1182-1191, 2017 Aug.
[Is] ISSN:1546-1718
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Immunoglobulins are the effector molecules of the adaptive humoral immune system. In a genome-wide association study of 19,219 individuals, we found 38 new variants and replicated 5 known variants associating with IgA, IgG or IgM levels or with composite immunoglobulin traits, accounted for by 32 loci. Variants at these loci also affect the risk of autoimmune diseases and blood malignancies and influence blood cell development. Notable associations include a rare variant at RUNX3 decreasing IgA levels by shifting isoform proportions (rs188468174[C>T]: P = 8.3 × 10 , ß = -0.90 s.d.), a rare in-frame deletion in FCGR2B abolishing IgG binding to the encoded receptor (p.Asn106del: P = 4.2 × 10 , ß = 1.03 s.d.), four IGH locus variants influencing class switching, and ten new associations with the HLA region. Our results provide new insight into the regulation of humoral immunity.
[Mh] Termos MeSH primário: Variação Genética
Imunoglobulinas/genética
[Mh] Termos MeSH secundário: Estudos de Coortes
Feminino
Predisposição Genética para Doença
Estudo de Associação Genômica Ampla
Hematopoese/genética
Seres Humanos
Islândia
Imunidade Humoral/genética
Switching de Imunoglobulina/genética
Isotipos de Imunoglobulinas/genética
Masculino
Polimorfismo de Nucleotídeo Único
Suécia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin Isotypes); 0 (Immunoglobulins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1038/ng.3897


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[PMID]:28446565
[Au] Autor:Belmar NA; Chan SW; Fox MI; Samayoa JA; Stickler MM; Tran NN; Akamatsu Y; Hollenbaugh D; Harding FA; Alvarez HM
[Ad] Endereço:Oncology Biologics Department, AbbVie Biotherapeutics Inc., Redwood City, CA 94063.
[Ti] Título:Murinization and H Chain Isotype Matching of the Anti-GITR Antibody DTA-1 Reduces Immunogenicity-Mediated Anaphylaxis in C57BL/6 Mice.
[So] Source:J Immunol;198(11):4502-4512, 2017 Jun 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent advances in immuno-oncology have shown that the immune system can be activated to induce long-term, durable antitumor responses. For immuno-oncology drug development, immune activation is often explored using rat Abs in immunocompetent mouse models. Although these models can be used to show efficacy, antidrug immune responses to experimental protein-based therapeutics can arise. Immunogenicity of surrogate Abs may therefore represent an important obstacle to the evaluation of the antitumor efficacy of immunomodulator Abs in syngeneic models. A recent publication has shown that anti-glucocorticoid-induced TNFR family-related protein agonistic Ab DTA-1 (rat or murinized IgG2a) can induce the development of anaphylaxis in C57BL/6 mice upon repeated i.p. dosing because of an anti-idiotypic anti-drug Ab immune response. This study was undertaken to address the impact of the immunogenicity derived from the Fc and variable domains. To this end, chimerized (rat V domains/mouse constant regions) and murinized (95% mouse sequence) DTA-1-based surrogate Abs with a murine IgG2c H chain isotype were created. Chimerization and murinization of DTA-1 did not affect receptor binding and glucocorticoid-induced TNFR family-related protein-induced T cell agonistic properties. Similar in vivo antitumor efficacy and intratumoral CD8 /regulatory T cells were also observed. Finally, treatment of C57BL/6 mice with the chimerized and murinized DTA-1 Abs on a C57BL/6-matched IgG2c isotype resulted in reduced development and severity of anaphylaxis as measured by decline of body temperature, behavioral effects, serum IL-4, IgE, and anti-drug Ab levels. These results suggest that careful murinization and selection of a strain-matched H chain isotype are critical to generate ideal surrogate Abs for testing immuno-oncology mechanisms in vivo.
[Mh] Termos MeSH primário: Anafilaxia/imunologia
Proteína Relacionada a TNFR Induzida por Glucocorticoide/imunologia
Isotipos de Imunoglobulinas/imunologia
[Mh] Termos MeSH secundário: Animais
Linfócitos T CD8-Positivos/imunologia
Linhagem Celular Tumoral
Interleucina-4/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Ratos
Receptores de IgG/imunologia
Linfócitos T Reguladores/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucocorticoid-Induced TNFR-Related Protein); 0 (IgG2c receptor); 0 (Immunoglobulin Isotypes); 0 (Receptors, IgG); 0 (Tnfrsf18 protein, mouse); 207137-56-2 (Interleukin-4)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601512


  9 / 3863 MEDLINE  
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[PMID]:28416601
[Au] Autor:Boyer F; Boutouil H; Dalloul I; Dalloul Z; Cook-Moreau J; Aldigier JC; Carrion C; Herve B; Scaon E; Cogné M; Péron S
[Ad] Endereço:Université de Limoges, Contrôle de la Réponse Immune B et Lymphoproliférations, UMR 7276, F-87000 Limoges, France.
[Ti] Título:CSReport: A New Computational Tool Designed for Automatic Analysis of Class Switch Recombination Junctions Sequenced by High-Throughput Sequencing.
[So] Source:J Immunol;198(10):4148-4155, 2017 May 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:B cells ensure humoral immune responses due to the production of Ag-specific memory B cells and Ab-secreting plasma cells. In secondary lymphoid organs, Ag-driven B cell activation induces terminal maturation and Ig isotype class switch (class switch recombination [CSR]). CSR creates a virtually unique locus in every B cell clone by intrachromosomal recombination between two switch (S) regions upstream of each C region gene. Amount and structural features of CSR junctions reveal valuable information about the CSR mechanism, and analysis of CSR junctions is useful in basic and clinical research studies of B cell functions. To provide an automated tool able to analyze large data sets of CSR junction sequences produced by high-throughput sequencing (HTS), we designed CSReport, a software program dedicated to support analysis of CSR recombination junctions sequenced with a HTS-based protocol (Ion Torrent technology). CSReport was assessed using simulated data sets of CSR junctions and then used for analysis of Sµ-Sα and Sµ-Sγ1 junctions from CH12F3 cells and primary murine B cells, respectively. CSReport identifies junction segment breakpoints on reference sequences and junction structure (blunt-ended junctions or junctions with insertions or microhomology). Besides the ability to analyze unprecedentedly large libraries of junction sequences, CSReport will provide a unified framework for CSR junction studies. Our results show that CSReport is an accurate tool for analysis of sequences from our HTS-based protocol for CSR junctions, thereby facilitating and accelerating their study.
[Mh] Termos MeSH primário: Sequenciamento de Nucleotídeos em Larga Escala
Switching de Imunoglobulina/genética
Recombinação Genética
Software
[Mh] Termos MeSH secundário: Linfócitos B/imunologia
Quebras de DNA de Cadeia Dupla
Isotipos de Imunoglobulinas/genética
Região de Troca de Imunoglobulinas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin Isotypes)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601924


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[PMID]:28321612
[Au] Autor:Suri D; Bhattad S; Sharma A; Gupta A; Rawat A; Sehgal S; Singh S; Gupta S
[Ad] Endereço:Pediatric Allergy and Immunology Unit, Advanced Pediatrics Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India. surideepti@gmail.com.
[Ti] Título:Serial Serum Immunoglobulin G (IgG) Trough Levels in Patients with X-linked Agammaglobulinemia on Replacement Therapy with Intravenous Immunoglobulin: Its Correlation with Infections in Indian Children.
[So] Source:J Clin Immunol;37(3):311-318, 2017 Apr.
[Is] ISSN:1573-2592
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Patients with primary antibody deficiency (PAD) are being increasingly diagnosed in the developing world. However, care of these children continues to remain suboptimal due to financial and social constraints. Immunoglobulin (Ig) trough level is an important predicting factor for infections in children on replacement immunoglobulin therapy. There are no data on this aspect from the developing world. Therefore, we studied serial immunoglobulin G (IgG) trough levels in 14 children with X-linked agammaglobulinemia (XLA) receiving replacement intravenous immunoglobulin (IVIG). Infections during the course of enrolment were documented prospectively. Mean age at the time of diagnosis was 5.1 years (range 2-11 years). Mean time from onset of symptoms and initiation of therapy was 3.3 years. Two children had established chronic lung disease prior to enrolment. Total numbers of major and minor infections were 7 and 40, respectively. At a mean dose of 414 mg/kg/month of IVIG, mean trough IgG level was 435 mg/dl. Median IgG trough levels during the episodes of major and minor infections were 244 and 335 mg/dl, respectively. An escalation in IVIG dose of 100 mg/kg produced an increase in serum IgG levels by 53.6 mg/dl. Median trough IgG level of 354 mg/dl was found to be protective with 64% sensitivity and 75% specificity. A median dose of 397 mg/kg was required to keep children free of infections. Despite financial constraints and several challenges in the context of a developing country, children with XLA have good outcome on replacement immunoglobulin therapy. Furthermore, mean biological trough IgG levels are much lower than reported in for Western patients; however, studies involving larger number of subjects are required in future to draw firm conclusions.
[Mh] Termos MeSH primário: Agamaglobulinemia/sangue
Agamaglobulinemia/tratamento farmacológico
Doenças Genéticas Ligadas ao Cromossomo X/sangue
Doenças Genéticas Ligadas ao Cromossomo X/tratamento farmacológico
Imunoglobulina G/sangue
Imunoglobulinas Intravenosas/uso terapêutico
[Mh] Termos MeSH secundário: Adolescente
Agamaglobulinemia/complicações
Agamaglobulinemia/diagnóstico
Criança
Pré-Escolar
Feminino
Seguimentos
Doenças Genéticas Ligadas ao Cromossomo X/complicações
Doenças Genéticas Ligadas ao Cromossomo X/diagnóstico
Seres Humanos
Isotipos de Imunoglobulinas/sangue
Índia
Infecção/etiologia
Masculino
Fenótipo
Curva ROC
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin G); 0 (Immunoglobulin Isotypes); 0 (Immunoglobulins, Intravenous)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171022
[Lr] Data última revisão:
171022
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1007/s10875-017-0379-5



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