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[PMID]:28181387
[Au] Autor:Gato WE; Hunter DA; Byrd IC; Mays CA; Yau W; Wu J
[Ad] Endereço:Department of Chemistry, Georgia Southern University, Statesboro, GA, 30458.
[Ti] Título:Assessment of the short-term toxicity of TiO nanofiber in Sprague Dawley rats.
[So] Source:Environ Toxicol;32(6):1775-1783, 2017 Jun.
[Is] ISSN:1522-7278
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Synthetic nanomaterials have many unique chemical and physical properties, mainly due to their high specific surface area and quantum confinement effect. Specifically, titanium dioxide (TiO ) nanomaterial has high stability, anticorrosive, and photocatalytic properties. However, there are concerns over adverse biological effects resulting from bioeffects. This study was to investigate adverse effects associated with acute ingestion of TiO nanofiber (TDNF). TDNF was fabricated via electrospinning method, followed by dissolution in water. Six- to seven-week-old male Sprague Dawley rats were exposed to a total of 0, 40, and 60 ppm of TDNF for 2 weeks via oral gavage. Serum total protein and weight gain during the course of this study displayed marginal concentration-dependent alterations. These findings were followed by a global gene expression analysis to identify which transcripts might be responsive to TNDF toxicity. Differentially expressed mRNA levels were dose-dependently higher in animals exposed to TNDF. The majority of the affected genes were biochemically involved in immune response and inflammation. We believe this is due to the fact that TNDF is unable to penetrate the cell and forms phagocytosis sites that trigger inflammatory and immune response. All results taken together, short-term ingestion of TNDF produced marginal effects indicative of inflammation. Finally, the broad gene expression data were validated through quantification of immunoglobulin heavy chain alpha (Igha). Igha gene was upregulated in treated groups, showing similar expression patterns to the global gene expression data.
[Mh] Termos MeSH primário: Expressão Gênica/efeitos dos fármacos
Cadeias alfa de Imunoglobulina/genética
Nanofibras/toxicidade
Pneumonia/virologia
Titânio/toxicidade
[Mh] Termos MeSH secundário: Administração Oral
Animais
Relação Dose-Resposta a Droga
Estudo de Associação Genômica Ampla
Masculino
Pneumonia/imunologia
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin alpha-Chains); 15FIX9V2JP (titanium dioxide); D1JT611TNE (Titanium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.1002/tox.22400


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[PMID]:28069266
[Au] Autor:Vignon M; Cohen C; Faguer S; Noel LH; Guilbeau C; Rabant M; Higgins S; Hummel A; Hertig A; Francois H; Lequintrec M; Vilaine E; Knebelmann B; Pourrat J; Chauveau D; Goujon JM; Javaugue V; Touchard G; El Karoui K; Bridoux F
[Ad] Endereço:Department of Nephrology, Hôpital Necker Enfants Malades, Université Paris Descartes, Paris, France. Electronic address: marguerite.vignon@aphp.fr.
[Ti] Título:The clinicopathologic characteristics of kidney diseases related to monotypic IgA deposits.
[So] Source:Kidney Int;91(3):720-728, 2017 Mar.
[Is] ISSN:1523-1755
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Monoclonal gammopathy of renal significance (MGRS) regroups renal disorders caused by a monoclonal immunoglobulin without overt hematological malignancy. MGRS includes tubular disorders, glomerular disorders with organized deposits, and glomerular disorders with non-organized deposits, such as proliferative glomerulonephritis with monoclonal IgG deposits. Since glomerular involvement related to monotypic IgA deposits is poorly described we performed retrospective analysis and defined clinico-biological characteristics, renal pathology, and outcome in 19 referred patients. This analysis allowed distinction between 2 types of glomerulopathies, α-heavy chain deposition disease (5 patients) and glomerulonephritis with monotypic IgA deposits (14 patients) suggestive of IgA-proliferative glomerulonephritis with monoclonal immunoglobulin deposits in 12 cases. Clinicopathologic characteristics of α-heavy chain deposition disease resemble those of the γ-heavy chain disease, except for a higher frequency of extra-capillary proliferation and extra-renal involvement. IgA-proliferative glomerulonephritis with monoclonal immunoglobulin deposits should be differentiated from diseases with polytypic IgA deposits, given distinct clinical, histological, and pathophysiological features. Similarly to IgG-proliferative glomerulonephritis with monoclonal immunoglobulin deposits, overt hematological malignancy was infrequent, but sensitive serum and bone marrow studies revealed a subtle plasma cell proliferation in most patients with IgA-proliferative glomerulonephritis with monoclonal immunoglobulin deposits. Anti-myeloma agents appeared to favorably influence renal prognosis. Thus, potential progression towards symptomatic IgA multiple myeloma suggests that careful hematological follow-up is mandatory. This series expands the spectrum of renal disease in MGRS.
[Mh] Termos MeSH primário: Glomerulonefrite por IGA/imunologia
Glomerulonefrite/imunologia
Doença das Cadeias Pesadas/imunologia
Imunoglobulina A/análise
Rim/imunologia
Mieloma Múltiplo/imunologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores/análise
Biópsia
Proliferação Celular
Diagnóstico Diferencial
Progressão da Doença
Feminino
Imunofluorescência
França
Glomerulonefrite/tratamento farmacológico
Glomerulonefrite/patologia
Glomerulonefrite por IGA/tratamento farmacológico
Glomerulonefrite por IGA/patologia
Doença das Cadeias Pesadas/tratamento farmacológico
Doença das Cadeias Pesadas/patologia
Seres Humanos
Cadeias alfa de Imunoglobulina/análise
Cadeias gama de Imunoglobulina/análise
Rim/efeitos dos fármacos
Rim/ultraestrutura
Masculino
Meia-Idade
Mieloma Múltiplo/tratamento farmacológico
Mieloma Múltiplo/patologia
Valor Preditivo dos Testes
Prognóstico
Estudos Retrospectivos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Biomarkers); 0 (Immunoglobulin A); 0 (Immunoglobulin alpha-Chains); 0 (Immunoglobulin gamma-Chains); 0 (heavy chain disease proteins, human)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE


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[PMID]:26834022
[Au] Autor:Wilbers RH; Westerhof LB; van Raaij DR; van Adrichem M; Prakasa AD; Lozano-Torres JL; Bakker J; Smant G; Schots A
[Ad] Endereço:Laboratory of Nematology, Plant Sciences Department, Wageningen University and Research Centre, Wageningen, The Netherlands.
[Ti] Título:Co-expression of the protease furin in Nicotiana benthamiana leads to efficient processing of latent transforming growth factor-ß1 into a biologically active protein.
[So] Source:Plant Biotechnol J;14(8):1695-704, 2016 Aug.
[Is] ISSN:1467-7652
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Transforming growth factor beta (TGF-ß) is a signalling molecule that plays a key role in developmental and immunological processes in mammals. Three TGF-ß isoforms exist in humans, and each isoform has unique therapeutic potential. Plants offer a platform for the production of recombinant proteins, which is cheap and easy to scale up and has a low risk of contamination with human pathogens. TGF-ß3 has been produced in plants before using a chloroplast expression system. However, this strategy requires chemical refolding to obtain a biologically active protein. In this study, we investigated the possibility to transiently express active human TGF-ß1 in Nicotiana benthamiana plants. We successfully expressed mature TGF-ß1 in the absence of the latency-associated peptide (LAP) using different strategies, but the obtained proteins were inactive. Upon expression of LAP-TGF-ß1, we were able to show that processing of the latent complex by a furin-like protease does not occur in planta. The use of a chitinase signal peptide enhanced the expression and secretion of LAP-TGF-ß1, and co-expression of human furin enabled the proteolytic processing of latent TGF-ß1. Engineering the plant post-translational machinery by co-expressing human furin also enhanced the accumulation of biologically active TGF-ß1. This engineering step is quite remarkable, as furin requires multiple processing steps and correct localization within the secretory pathway to become active. Our data demonstrate that plants can be a suitable platform for the production of complex proteins that rely on specific proteolytic processing.
[Mh] Termos MeSH primário: Furina/metabolismo
Proteínas Recombinantes de Fusão/metabolismo
Tabaco/genética
Fator de Crescimento Transformador beta1/metabolismo
Fator de Crescimento Transformador beta1/farmacologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Células Epiteliais/efeitos dos fármacos
Furina/genética
Seres Humanos
Cadeias alfa de Imunoglobulina/genética
Cadeias alfa de Imunoglobulina/metabolismo
Vison
Folhas de Planta/genética
Folhas de Planta/metabolismo
Plantas Geneticamente Modificadas
Redobramento de Proteína
Sinais Direcionadores de Proteínas/genética
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/farmacologia
Tabaco/metabolismo
Fator de Crescimento Transformador beta1/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin alpha-Chains); 0 (Protein Sorting Signals); 0 (Recombinant Fusion Proteins); 0 (Transforming Growth Factor beta1); EC 3.4.21.75 (Furin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE
[do] DOI:10.1111/pbi.12530


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[PMID]:26528943
[Au] Autor:Campos-Rodríguez R; Godínez-Victoria M; Arciniega-Martínez IM; Reséndiz-Albor AA; Reyna-Garfias H; Cruz-Hernández TR; Drago-Serrano ME
[Ad] Endereço:Escuela Superior de Meidicina, Instituto Politécnico Nacional, Sección de Estudios de Posgrado e Investigación, Mexico, Mexico.
[Ti] Título:Protective Effect of Moderate Exercise for BALB/c Mice with Salmonella Typhimurium Infection.
[So] Source:Int J Sports Med;37(1):63-70, 2016 Jan.
[Is] ISSN:1439-3964
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Moderate exercise enhances resistance to pathogen-associated infections. However, its influence on intestinal IgA levels and resistance to Salmonella typhimurium in mice has not been reported. The aim of this study was to assess the impact of moderate exercise on bacterial resistance and the intestinal-IgA response in a murine typhoid model. Sedentary and exercised (under a protocol of moderate swimming) BALB/c mice were orally infected with Salmonella typhimurium and sacrificed on days 7 or 14 post-infection (n=5 per group). Compared with infected sedentary mice, infected exercised animals had i) lower intestinal and systemic bacterial loads; ii) higher total and specific intestinal-IgA levels, iii) a higher percentage of IgA plasma cells in lamina propria; iv) a higher level on day 7 and lower level on day 14 of intestinal α- and J-chain mRNA and plasma corticosterone, v) unchanged mRNA expression of intestinal pIgR, and vi) a higher mRNA expression of liver pIgR, α-chain and J-chain on day 7. Hence, it is likely that an increase in corticosterone levels (stress response) induced by moderate exercise increased intestinal IgA levels by enabling greater liver expression of pIgR mRNA, leading to a rise in IgA transcytosis from the liver to intestine. The overall effect of these changes is an enhanced resistance to infection.
[Mh] Termos MeSH primário: Resistência à Doença/fisiologia
Imunoglobulina A/metabolismo
Intestinos/metabolismo
Condicionamento Físico Animal/fisiologia
Infecções por Salmonella/prevenção & controle
Salmonella typhimurium
[Mh] Termos MeSH secundário: Animais
Carga Bacteriana
Corticosterona/sangue
Modelos Animais de Doenças
Cadeias J de Imunoglobulina/metabolismo
Cadeias alfa de Imunoglobulina/metabolismo
Intestinos/microbiologia
Fígado/metabolismo
Masculino
Camundongos Endogâmicos BALB C
RNA Mensageiro/metabolismo
Receptores de Imunoglobulina Polimérica/metabolismo
Natação/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immunoglobulin A); 0 (Immunoglobulin J-Chains); 0 (Immunoglobulin alpha-Chains); 0 (RNA, Messenger); 0 (Receptors, Polymeric Immunoglobulin); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151104
[St] Status:MEDLINE
[do] DOI:10.1055/s-0035-1559697


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[PMID]:26485749
[Au] Autor:Evans JA; Jenner EL; Carr Smith HD; Berlanga O; Harding SJ
[Ti] Título:Quantification of ß-region IgA monoclonal proteins - should we include immunochemical Hevylite® measurements? Point.
[So] Source:Clin Chem Lab Med;54(6):1053-7, 2016 Jun 01.
[Is] ISSN:1437-4331
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Accurate measurement of IgA monoclonal proteins presents a significant challenge to laboratory staff. IgA heavy/light chain (Hevylite, HLC) analysis is an alternative methodology for monoclonal protein assessment, giving an independent measure of IgAκ and IgAλ concentrations. Clonality is assessed by calculating the ratio of involved immunoglobulin to background uninvolved immunoglobulin concentrations (e.g. IgAκ/IgAλ in an IgAκ patient). Here we discuss the challenges faced by the laboratory in IgA monoclonal protein assessment, and compare the performance of Hevylite assays with electrophoresis and total IgA results. We present data which validates the use of Hevylite for response assessment: in most cases, Hevylite provides comparable response assignment to that provided by serum protein electrophoresis (SPE) and total IgA; in other cases Hevylite provides additional information, such as detection of residual disease or relapse.
[Mh] Termos MeSH primário: Imunoensaio/métodos
Imunoglobulina A/sangue
[Mh] Termos MeSH secundário: Seres Humanos
Cadeias alfa de Imunoglobulina/sangue
Cadeias kappa de Imunoglobulina/sangue
Cadeias lambda de Imunoglobulina/sangue
Paraproteinemias/diagnóstico
Paraproteinemias/imunologia
Paraproteínas/análise
Recidiva
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin A); 0 (Immunoglobulin alpha-Chains); 0 (Immunoglobulin kappa-Chains); 0 (Immunoglobulin lambda-Chains); 0 (Paraproteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151021
[St] Status:MEDLINE


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[PMID]:25391515
[Au] Autor:Serone E; Daleno C; Principi N; Porretti L; Iacoacci V; Gargioli C; Magrini A; Massoud R; D'Addabbo P; Cattalini M; Giambra V; Plebani A; Esposito S; Frezza D
[Ti] Título:The change in Ig regulation from children to adults disconnects the correlation with the 3'RR hs1.2 polymorphism.
[So] Source:BMC Immunol;15:45, 2014 Nov 13.
[Is] ISSN:1471-2172
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In the immune system, the serum levels of immunoglobulin (Ig) increase gradually during ageing. Through B cell development, the Ig heavy chain expression is modulated by a regulatory region at the 3' of the constant alpha gene (3'RR), in single copy in rodents and, due to a large duplication, in two copies in apes. The human 3'RR1 and 3'RR2 are both characterized by three enhancers, the central of which, namely hs1.2, is highly polymorphic. Human hs1.2 has four different variants with unique binding sites for transcription factors (e.g. NF-kB and SP1) and shows variable allelic frequencies in populations with immune disorders. In previous works, we have reported that in several autoimmune diseases the *2 allele of hs1.2 is genetically associated to high level of IgM in peripheral blood. In subjects with altered levels of circulating Ig, an increased level was associated to *2 allele of hs1.2 and low levels corresponded to high frequency of *1 allele. RESULTS: We have correlated the allelic frequencies of hs1.2 with IgM, IgG and IgA serum concentrations in two cohorts of healthy people of different age and after three years follow-up in children homozygous for the allele. Here we show that when the expression levels of Ig in children are low and medium, the frequencies of *1 and *2 alleles are the same. Instead, when the Ig expression levels are high, there is a significantly higher frequency of the allele *2. The follow-up of children homozygous for *1 and *2 alleles showed that the increase or decrease of circulating Ig was not dependent on the number of circulating mature B cells. CONCLUSIONS: These data support the idea that under physiologic condition there is a switch of regulative pathways involved in the maturation of Ig during ageing. This mechanism is evidenced by hs1.2 variants that in children but not in adults participate to Ig production, coordinating the three class levels.
[Mh] Termos MeSH primário: Elementos Facilitadores Genéticos/genética
Cadeias alfa de Imunoglobulina/genética
Polimorfismo Genético
[Mh] Termos MeSH secundário: Adulto
Criança
Pré-Escolar
Feminino
Seguimentos
Frequência do Gene
Seres Humanos
Cadeias alfa de Imunoglobulina/sangue
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin alpha-Chains)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141114
[St] Status:MEDLINE
[do] DOI:10.1186/s12865-014-0045-0


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[PMID]:24979072
[Au] Autor:Insenser M; Montes-Nieto R; Martínez-García MÁ; Durán EF; Santiuste C; Gómez V; Kline JA; Escobar-Morreale HF; Jiménez D
[Ad] Endereço:Diabetes, Obesity and Human Reproduction Research Group, Department of Endocrinology & Nutrition, Hospital Universitario Ramón y Cajal & Universidad de Alcalá & Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) & Centro de Investigación Biomédica en Red Diabetes y Enfermeda
[Ti] Título:Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: a nontargeted proteomic study.
[So] Source:PLoS One;9(6):e100902, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Risk stratification of patients with pulmonary embolism (PE) may identify patients at high risk of early death who may benefit from more intensive surveillance or aggressive therapy. Nontargeted proteomics may identify biomarkers useful for the risk stratification of patients with acute symptomatic pulmonary embolism (PE). We studied 6 patients presenting with low-risk PE and 6 patients presenting with intermediate (n = 3) or high-risk (n = 3) PE. Two-dimensional difference gel electrophoresis was used to compare their plasma protein abundances. Candidate protein markers were identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry. A panel of four biomarkers (haptoglobin, hemopexin, α2-macroglobulin, and Ig α1-chain C region) showed differences in plasma abundance among patients with acute symptomatic PE of different severity. Haptoglobin and hemopexin were decreased, whereas α2-macroglobulin and Ig α1-chain C region were increased, in patients with high or intermediate-risk PE compared with low-risk PE patient. In a separate clinical population consisting of 104 adults with acute PE, serum haptoglobin concentrations had an 85% chance of correctly identifying patients with high-risk PE according to receiving operating characteristics curve analysis. Moreover, serum haptoglobin concentrations ≤1 g/l showed an 80% sensitivity and a 96% specificity for the diagnosis of high-risk PE. Nontargeted proteomics identified protein biomarkers for the severity of PE that are involved in iron metabolism pathways and acute-phase response. Among them, reduced serum haptoglobin concentrations show a high accuracy for the biochemical detection of high-risk PE.
[Mh] Termos MeSH primário: Haptoglobinas/metabolismo
Embolia Pulmonar/sangue
Embolia Pulmonar/diagnóstico
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Biomarcadores/sangue
Eletroforese em Gel Bidimensional
Feminino
Hemopexina/metabolismo
Seres Humanos
Cadeias alfa de Imunoglobulina/sangue
Masculino
Prognóstico
Proteoma/metabolismo
Embolia Pulmonar/patologia
Risco
Sensibilidade e Especificidade
Índice de Gravidade de Doença
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
alfa-Macroglobulinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Haptoglobins); 0 (Immunoglobulin alpha-Chains); 0 (Proteome); 0 (alpha-Macroglobulins); 9013-71-2 (Hemopexin)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140701
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0100902


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[PMID]:24738839
[Au] Autor:Carneiro LG; Nouh H; Salih E
[Ad] Endereço:Department of Periodontology and Oral Biology, School of Dental Medicine, Boston University, Boston, MA, USA.
[Ti] Título:Quantitative gingival crevicular fluid proteome in health and periodontal disease using stable isotope chemistries and mass spectrometry.
[So] Source:J Clin Periodontol;41(8):733-47, 2014 Aug.
[Is] ISSN:1600-051X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIM: Application of quantitative stable isotope-labelling chemistries and mass spectrometry (MS) to determine alterations in gingival crevicular fluid (GCF) proteome in periodontal disease. MATERIAL AND METHODS: Quantitative proteome of GCF from 40 healthy individuals versus 40 patients with periodontal disease was established using 320 GCF samples and stable isotope-labelling reagents, ICAT and mTRAQ, with MS technology and validated by enzyme-linked immunosorbent methods. RESULTS: We have identified 238 distinct proteins of which 180 were quantified in GCF of both healthy and periodontal patients with additional 26 and 32 distinct proteins that were found only in GCF of healthy or periodontal patients. In addition, 42 pathogenic bacterial proteins and 11 yeast proteins were quantified. The data highlighted a series of proteins not quantified previously by large-scale MS approaches in GCF with relevance to periodontal disease, such as host-derived Ig alpha-2 chain C, Kallikrein-4, S100-A9, transmembrane proteinase 13, peptidase S1 domain, several collagen types and pathogenic bacterial proteins, e.g. formamidase, leucine aminopeptidase and virulence factor OMP85. CONCLUSIONS: The innovative analytical approaches provided detailed novel changes in both host and microbial derived GCF proteomes of periodontal patients. The study defined 50 host and 16 pathogenic bacterial proteins significantly elevated in periodontal disease most of which were novel with significant potential for application in the clinical arena of periodontal disease.
[Mh] Termos MeSH primário: Líquido do Sulco Gengival/química
Doenças Periodontais/metabolismo
Proteoma/análise
[Mh] Termos MeSH secundário: Adulto
Albuminas/análise
Amidoidrolases/análise
Proteínas da Membrana Bacteriana Externa/análise
Proteínas de Bactérias/análise
Calgranulina B/análise
Cromatografia Líquida
Colágeno/análise
Eletroforese em Gel de Poliacrilamida
Feminino
Proteínas Fúngicas/análise
Seres Humanos
Cadeias alfa de Imunoglobulina/análise
Isótopos
Calicreínas/análise
Leucil Aminopeptidase/análise
Masculino
Espectrometria de Massas
Proteínas de Membrana/análise
Meia-Idade
Doenças Periodontais/microbiologia
Serina Endopeptidases/análise
Albumina Sérica/análise
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Albumins); 0 (Bacterial Outer Membrane Proteins); 0 (Bacterial Proteins); 0 (Calgranulin B); 0 (Fungal Proteins); 0 (Immunoglobulin alpha-Chains); 0 (Isotopes); 0 (Membrane Proteins); 0 (Proteome); 0 (Serum Albumin); 9007-34-5 (Collagen); EC 3.4.11.1 (Leucyl Aminopeptidase); EC 3.4.21.- (Kallikreins); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.- (TMPRSS13 protein, human); EC 3.4.21.- (kallikrein 4); EC 3.5.- (Amidohydrolases); EC 3.5.1.49 (formamidase)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:140418
[St] Status:MEDLINE
[do] DOI:10.1111/jcpe.12262


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[PMID]:24185710
[Au] Autor:Duan Z; Zheng H; Xu S; Jiang Y; Liu H; Li M; Hu D; Li W; Bode AM; Dong Z; Cao Y
[Ad] Endereço:1] Laboratory of Tumor Molecular Biology, Cancer Research Institute, Central South University, Changsha, China [2] Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha, China [3] Key Laboratory of Carcinogenesis, Ministry of Health, Changsha, China.
[Ti] Título:Activation of the Ig Iα1 promoter by the transcription factor Ets-1 triggers Ig Iα1-Cα1 germline transcription in epithelial cancer cells.
[So] Source:Cell Mol Immunol;11(2):197-205, 2014 Mar.
[Is] ISSN:2042-0226
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Immunoglobulins (Igs) are known to be synthesized and secreted only by B lymphocytes. Class switch recombination (CSR) is a key event that enables B cells to express Igs, and one of the crucial steps for CSR initiation is the germline transcription of Ig genes. Surprisingly, recent studies have demonstrated that the Ig genes are also expressed in some epithelial cancer cells; however, the mechanisms underlying how cancer cells initiate CSR and express Igs are still unknown. In this study, we confirmed that the Ig Iα1 promoter in cancer cell lines was activated by the Ets-1 transcription factor, and the activity of the Ig Iα1 promoter and Ig Iα1-Cα1 germline transcription were attenuated after knockdown of Ets-1 by specific small interfering RNAs (siRNA). Furthermore, the expression of Ets-1 and Igα heavy chain in cancer cells was dose dependently upregulated by TGF-ß1. These results indicate that activation of the Ig Iα1 promoter by the transcription factor Ets-1 is a critical pathway and provides a novel mechanism for Ig expression in non-B cell cancers.
[Mh] Termos MeSH primário: Carcinoma/imunologia
Imunoglobulina A/metabolismo
Regiões Constantes de Imunoglobulina/metabolismo
Cadeias alfa de Imunoglobulina/metabolismo
Proteína Proto-Oncogênica c-ets-1/metabolismo
[Mh] Termos MeSH secundário: Células Epiteliais/imunologia
Regulação Neoplásica da Expressão Gênica/genética
Células HeLa
Seres Humanos
Imunoglobulina A/genética
Regiões Constantes de Imunoglobulina/genética
Cadeias alfa de Imunoglobulina/genética
Especificidade de Órgãos
Regiões Promotoras Genéticas/genética
Proteína Proto-Oncogênica c-ets-1/genética
RNA Interferente Pequeno/genética
Ativação Transcricional/genética
Fator de Crescimento Transformador beta1/imunologia
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (ETS1 protein, human); 0 (Immunoglobulin A); 0 (Immunoglobulin Constant Regions); 0 (Immunoglobulin alpha-Chains); 0 (Proto-Oncogene Protein c-ets-1); 0 (RNA, Small Interfering); 0 (Transforming Growth Factor beta1)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131105
[St] Status:MEDLINE
[do] DOI:10.1038/cmi.2013.52


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[PMID]:23913047
[Au] Autor:Castello A; Gaya M; Tucholski J; Oellerich T; Lu KH; Tafuri A; Pawson T; Wienands J; Engelke M; Batista FD
[Ad] Endereço:Lymphocyte Interaction Laboratory, London Research Institute-Cancer Research UK, London, UK.
[Ti] Título:Nck-mediated recruitment of BCAP to the BCR regulates the PI(3)K-Akt pathway in B cells.
[So] Source:Nat Immunol;14(9):966-75, 2013 Sep.
[Is] ISSN:1529-2916
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The adaptor Nck links receptor signaling to cytoskeleton regulation. Here we found that Nck also controlled the phosphatidylinositol-3-OH kinase (PI(3)K)-kinase Akt pathway by recruiting the adaptor BCAP after activation of B cells. Nck bound directly to the B cell antigen receptor (BCR) via the non-immunoreceptor tyrosine-based activation motif (ITAM) phosphorylated tyrosine residue at position 204 in the tail of the immunoglobulin-α component. Genetic ablation of Nck resulted in defective BCR signaling, which led to hampered survival and proliferation of B cells in vivo. Indeed, antibody responses in Nck-deficient mice were also considerably impaired. Thus, we demonstrate a previously unknown adaptor function for Nck in recruiting BCAP to sites of BCR signaling and thereby modulating the PI(3)K-Akt pathway in B cells.
[Mh] Termos MeSH primário: Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Linfócitos B/metabolismo
Proteínas Oncogênicas/metabolismo
Fosfatidilinositol 3-Quinases/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Receptores de Antígenos de Linfócitos B/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/deficiência
Proteínas Adaptadoras de Transdução de Sinal/genética
Animais
Linfócitos B/imunologia
Feminino
Cadeias alfa de Imunoglobulina/química
Cadeias alfa de Imunoglobulina/metabolismo
Masculino
Camundongos
Camundongos Knockout
Proteínas Oncogênicas/deficiência
Proteínas Oncogênicas/genética
Fosforilação
Ligação Proteica
Linfócitos T/imunologia
Linfócitos T/metabolismo
Tirosina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Immunoglobulin alpha-Chains); 0 (Nck protein); 0 (Oncogene Proteins); 0 (Pik3ap1 protein, mouse); 0 (Receptors, Antigen, B-Cell); 42HK56048U (Tyrosine); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1311
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130806
[St] Status:MEDLINE
[do] DOI:10.1038/ni.2685



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