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[PMID]:29430664
[Au] Autor:Budzynska PM; Kyläniemi MK; Lassila O; Nera KP; Alinikula J
[Ad] Endereço:Department of Medical Microbiology and Immunology, Institute of Biomedicine, University of Turku, Turku, Finland.
[Ti] Título:BLIMP-1 is insufficient to induce antibody secretion in the absence of IRF4 in DT40 cells.
[So] Source:Scand J Immunol;87(3), 2018 Mar.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Differentiation of B cells into antibody-secreting cells (ASCs), plasmablasts and plasma cells is regulated by a network of transcription factors. Within this network, factors including PAX5 and BCL6 prevent ASC differentiation and maintain the B cell phenotype. In contrast, BLIMP-1 and high IRF4 expression promote plasma cell differentiation. BLIMP-1 is thought to induce immunoglobulin secretion, whereas IRF4 is needed for the survival of ASCs. The role of IRF4 in the regulation of antibody secretion has remained controversial. To study the role of IRF4 in the regulation of antibody secretion, we have created a double knockout (DKO) DT40 B cell line deficient in both IRF4 and BCL6. Although BCL6-deficient DT40 B cell line had upregulated BLIMP-1 expression and secreted antibodies, the DKO cell line did not. Even enforced BLIMP-1 expression in DKO cells or IRF4-deficient cells could not induce IgM secretion while in WT DT40 cells, it could. However, enforced IRF4 expression in DKO cells induced strong IgM secretion. Our findings support a model where IRF4 expression in addition to BLIMP-1 expression is required to induce robust antibody secretion.
[Mh] Termos MeSH primário: Anticorpos/imunologia
Formação de Anticorpos/genética
Proteínas Aviárias/genética
Linfócitos B/imunologia
Fatores Reguladores de Interferon/genética
Fator 1 de Ligação ao Domínio I Regulador Positivo/genética
[Mh] Termos MeSH secundário: Animais
Linfócitos B/citologia
Diferenciação Celular/imunologia
Linhagem Celular
Proliferação Celular
Galinhas
Técnicas de Inativação de Genes
Imunoglobulina M/biossíntese
Imunoglobulina M/imunologia
Fator de Transcrição PAX5/genética
Fator 1 de Ligação ao Domínio I Regulador Positivo/imunologia
Proteínas Proto-Oncogênicas c-bcl-6/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Avian Proteins); 0 (IRF4 protein, Gallus gallus); 0 (Immunoglobulin M); 0 (Interferon Regulatory Factors); 0 (PAX5 Transcription Factor); 0 (Proto-Oncogene Proteins c-bcl-6); EC 2.1.1.- (Positive Regulatory Domain I-Binding Factor 1)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12646


  2 / 48726 MEDLINE  
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[PMID]:29424453
[Au] Autor:Esenboga S; Cagdas D; Ozgur TT; Gur Cetinkaya P; Turkdemir LM; Sanal O; VanDerBurg M; Tezcan I
[Ad] Endereço:Department of Pediatrics, Division of Immunology, Hacettepe University Faculty of Medicine, Ankara, Turkey.
[Ti] Título:Clinical and genetic features of the patients with X-Linked agammaglobulinemia from Turkey: Single-centre experience.
[So] Source:Scand J Immunol;87(3), 2018 Mar.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:X-linked agammaglobulinemia is a primary immunodeficiency disorder resulting from BTK gene mutations. There are many studies in the literature suggesting contradictory ideas about phenotype-genotype correlation. The aim of this study was to identify the mutations and clinical findings of patients with XLA in Turkey, to determine long-term complications related to the disease and to analyse the phenotype-genotype correlation. Thirty-two patients with XLA diagnosed between 1985 and 2016 in Pediatric Immunology Department of Hacettepe University Ihsan Dogramaci Children's Hospital were investigated. A clinical survey including clinical features of the patients was completed, and thirty-two patients from 26 different families were included in the study. Getting early diagnosis and regular assessment with imaging techniques seem to be the most important issues for improving the health status of the patients with XLA. Early molecular analysis gives chance for definitive diagnosis and genetic counselling, but not for predicting the clinical severity and prognosis.
[Mh] Termos MeSH primário: Agamaglobulinemia/diagnóstico
Agamaglobulinemia/genética
Anticorpos/sangue
Doenças Genéticas Ligadas ao Cromossomo X/diagnóstico
Doenças Genéticas Ligadas ao Cromossomo X/genética
Proteínas Tirosina Quinases/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Agamaglobulinemia/patologia
Infecções Bacterianas/imunologia
Criança
Pré-Escolar
Doenças Genéticas Ligadas ao Cromossomo X/patologia
Seres Humanos
Imunoglobulina A/sangue
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Lactente
Estudos Retrospectivos
Turquia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (Immunoglobulin M); EC 2.7.10.1 (Agammaglobulinaemia tyrosine kinase); EC 2.7.10.1 (Protein-Tyrosine Kinases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12647


  3 / 48726 MEDLINE  
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[PMID]:29320815
[Au] Autor:Matin S; Shahbazi G; Namin ST; Moradpour R; Feizi F; Piri-Dogahe H
[Ad] Endereço:Department of Internal Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
[Ti] Título:Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran.
[So] Source:Korean J Parasitol;55(6):607-611, 2017 Dec.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.
[Mh] Termos MeSH primário: Aborto Habitual/etiologia
Aborto Espontâneo/etiologia
Anticorpos Antiprotozoários/sangue
Reação em Cadeia da Polimerase/métodos
Complicações Parasitárias na Gravidez/diagnóstico
Testes Sorológicos/métodos
Toxoplasma/imunologia
Toxoplasmose/diagnóstico
Toxoplasmose/parasitologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Biomarcadores/sangue
DNA de Protozoário/sangue
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Irã (Geográfico)/epidemiologia
Gravidez
Complicações Parasitárias na Gravidez/epidemiologia
Complicações Parasitárias na Gravidez/parasitologia
Toxoplasma/genética
Toxoplasmose/complicações
Toxoplasmose/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Biomarkers); 0 (DNA, Protozoan); 0 (Immunoglobulin G); 0 (Immunoglobulin M)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.6.607


  4 / 48726 MEDLINE  
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[PMID]:28453840
[Au] Autor:Young VP; Mariano MC; Tu CC; Allaire KM; Avdic S; Slobedman B; Spencer JV
[Ad] Endereço:Department of Biology, University of San Francisco, California, USA.
[Ti] Título:Modulation of the Host Environment by Human Cytomegalovirus with Viral Interleukin 10 in Peripheral Blood.
[So] Source:J Infect Dis;215(6):874-882, 2017 03 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Human cytomegalovirus (HCMV) is a herpesvirus with both lytic and latent life cycles. Human cytomegalovirus encodes 2 viral cytokines that are orthologs of human cellular interleukin 10 (cIL-10). Both cytomegalovirus interleukin 10 (cmvIL-10) and Latency-associated cytomegalovirus interleukin 10 (LAcmvIL-10) (collectively vIL-10) are expressed during lytic infection and cause immunosuppressive effects that impede virus clearance. LAcmvIL-10 is also expressed during latent infection of myeloid progenitor cells and monocytes and facilitates persistence. Here, we investigated whether vIL-10 could be detected during natural infection. Methods: Plasma from healthy blood donors was tested by enzyme-linked immunosorbent assay for anti-HCMV immunoglobulin G and immunoglobulin M and for cIL-10 and vIL-10 levels using a novel vIL-10 assay that detects cmvIL-10 and LAcmvIL-10, with no cross-reactivity to cIL-10. Results: vIL-10 was evident in HCMV+ donors (n = 19 of 26), at levels ranging 31-547 pg/mL. By comparison, cIL-10 was detected at lower levels ranging 3-69 pg/mL. There was a strong correlation between vIL-10 and cIL-10 levels (P = .01). Antibodies against vIL-10 were also detected and neutralized vIL-10 activity. Conclusions: vIL-10 was detected in peripheral blood of healthy blood donors. These findings suggest that vIL-10 may play a key role in sensing or modifying the host environment during latency and, therefore, may be a potential target for intervention strategies.
[Mh] Termos MeSH primário: Infecções por Citomegalovirus/sangue
Citomegalovirus/imunologia
Interleucina-10/sangue
Proteínas Virais/sangue
[Mh] Termos MeSH secundário: Anticorpos Antivirais/sangue
Reações Cruzadas
Infecções por Citomegalovirus/imunologia
Ensaio de Imunoadsorção Enzimática
Voluntários Saudáveis
Seres Humanos
Tolerância Imunológica
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Interleucina-10/imunologia
Monócitos/imunologia
Proteínas Virais/imunologia
Latência Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (CMV IL-10 protein, Cytomegalovirus); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (Viral Proteins); 130068-27-8 (Interleukin-10)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix043


  5 / 48726 MEDLINE  
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[PMID]:29342381
[Au] Autor:Kyle RA; Larson DR; Therneau TM; Dispenzieri A; Kumar S; Cerhan JR; Rajkumar SV
[Ad] Endereço:From the Divisions of Hematology (R.A.K., A.D., S.K., S.V.R.), Biostatistics (D.R.L., T.M.T.), and Epidemiology (J.R.C.), Mayo Clinic, Rochester, MN.
[Ti] Título:Long-Term Follow-up of Monoclonal Gammopathy of Undetermined Significance.
[So] Source:N Engl J Med;378(3):241-249, 2018 01 18.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Monoclonal gammopathy of undetermined significance (MGUS) occurs in approximately 3% of persons 50 years of age or older. METHODS: We studied 1384 patients who were residing in southeastern Minnesota and in whom MGUS was diagnosed at the Mayo Clinic in the period from 1960 through 1994; the median follow-up was 34.1 years (range, 0.0 to 43.6). The primary end point was progression to multiple myeloma or another plasma-cell or lymphoid disorder. RESULTS: During 14,130 person-years of follow-up, MGUS progressed in 147 patients (11%), a rate that was 6.5 times (95% confidence interval [CI], 5.5 to 7.7) as high as the rate in the control population. The risk of progression without accounting for death due to competing causes was 10% at 10 years, 18% at 20 years, 28% at 30 years, 36% at 35 years, and 36% at 40 years. Among patients with IgM MGUS, the presence of two adverse risk factors - namely, an abnormal serum free light-chain ratio (ratio of kappa to lambda free light chains) and a high serum monoclonal protein (M protein) level (≥1.5 g per deciliter) - was associated with a risk of progression at 20 years of 55%, as compared with 41% among patients who had one adverse risk factor and 19% among patients who had neither risk factor. Among patients with non-IgM MGUS, the risk of progression at 20 years was 30% among those who had the two risk factors, 20% among those who had one risk factor, and 7% among those who had neither risk factor. Patients with MGUS had shorter survival than was expected in the control population of Minnesota residents of matched age and sex (median, 8.1 vs. 12.4 years; P<0.001). CONCLUSIONS: Significant differences were noted in the risk of progression between patients with IgM MGUS and those with non-IgM MGUS. Overall survival was shorter among patients with MGUS than was expected in a matched control population. (Funded by the National Cancer Institute.).
[Mh] Termos MeSH primário: Progressão da Doença
Gamopatia Monoclonal de Significância Indeterminada/complicações
[Mh] Termos MeSH secundário: Adulto
Idoso
Exame de Medula Óssea
Feminino
Seguimentos
Glicoproteínas/sangue
Seres Humanos
Cadeias Leves de Imunoglobulina/sangue
Imunoglobulina M/sangue
Masculino
Meia-Idade
Gamopatia Monoclonal de Significância Indeterminada/mortalidade
Gamopatia Monoclonal de Significância Indeterminada/patologia
Mieloma Múltiplo/etiologia
Prognóstico
Modelos de Riscos Proporcionais
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Glycoproteins); 0 (Immunoglobulin Light Chains); 0 (Immunoglobulin M); 0 (protein M (glycoprotein))
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMoa1709974


  6 / 48726 MEDLINE  
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[PMID]:28457619
[Au] Autor:D'Arco C; Dattwyler RJ; Arnaboldi PM
[Ad] Endereço:Department of Microbiology and Immunology, School of Medicine, New York Medical College, Valhalla, NY 10595, United States.
[Ti] Título:Borrelia burgdorferi-specific IgA in Lyme Disease.
[So] Source:EBioMedicine;19:91-97, 2017 May.
[Is] ISSN:2352-3964
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The laboratory diagnosis of Lyme disease is currently dependent on the detection of IgM and IgG antibodies against Borrelia burgdorferi, the causative agent of the disease. The significance of serum IgA against B. burgdorferi remains unclear. The production of intrathecal IgA has been noted in patients with the late Lyme disease manifestation, neuroborreliosis, but production of antigen-specific IgA during early disease has not been evaluated. In the current study, we assessed serum IgA binding to the B. burgdorferi peptide antigens, C6, the target of the FDA-cleared C6 EIA, and FlaB(211-223)-modVlsE(275-291), a peptide containing a Borrelia flagellin epitope linked to a modified VlsE sequence, in patients with early and late Lyme disease. Specific IgA was detected in 59 of 152 serum samples (38.8%) from early Lyme disease patients. Approximately 50% of early Lyme disease patients who were seropositive for peptide-specific IgM and/or IgG were also seropositive for peptide-specific IgA. In a subpopulation of patients, high peptide-specific IgA could be correlated with disseminated disease, defined as multiple erythema migrans lesions, and neurological disease complications. These results suggest that there may be an association between elevated levels of antigen-specific IgA and particular disease manifestations in some patients with early Lyme disease.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/sangue
Borrelia burgdorferi/imunologia
Imunoglobulina A/sangue
Doença de Lyme/imunologia
[Mh] Termos MeSH secundário: Antígenos de Bactérias/imunologia
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Doença de Lyme/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (Immunoglobulin M)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  7 / 48726 MEDLINE  
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[PMID]:29274210
[Au] Autor:Bamba S; Zoungrana J; Nikièma Z; Sondo AK; Ndiaye JL; Bretagne S
[Ad] Endereço:Laboratory of Parasitology-Mycology, Higher Institute of Health Sciences, Polytechnic University, Rue Alwata Diawara, Bobo-Dioulasso, BP 1091, Burkina Faso
[Ti] Título:Impact of alternative treatment approach for cerebral toxoplasmosis among HIV/AIDS patients from a resource-poor setting in Burkina Faso
[So] Source:Ann Parasitol;63(3):173­181, 2017.
[Is] ISSN:2299-0631
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Cerebral toxoplasmosis is caused by the protozoan Toxoplasma gondii because of reactivation of latent tissue cysts in the Acquired Immunodeficiency Syndrome (AIDS) patients with severe immunosuppression. The objective of this study was to evaluate the benefit of co-trimoxazole in presumptive and prevention of cerebral toxoplasmosis in Human Immunodeficiency Virus (HIV)/AIDS patients at Bobo-Dioulasso Hospital in Burkina Faso from June 2012 to October 2014. ELISA and ELFA were performed on serum for the quantitative determination of IgG and IgM anti-T. gondii, respectively. The seroprevalence of toxoplasmosis was 29.3%. No IgM antibodies for T. gondii were found. Six patients with Toxoplasma-specific antibodies presented cerebral toxoplasmosis. All patients were infected by HIV-1 with the median of CD4+ T lymphocytes at 141 cells/µl. No patient was under antiretroviral therapy. No case of cerebral toxoplasmosis was noted in patients receiving co-trimoxazole in prevention. Presumptive treatment of cerebral toxoplasmosis with co-trimoxazole was effective in all patients with a significant clinical improvement in 83.3%. These results attest the benefit of cotrimoxazole in cerebral toxoplasmosis treatment in countries where drug resources are limited when sulfadiazine is not available. Ours finding highlight the importance of establishing toxoplasmosis chemoprophylaxis to HIV with severe immunosuppression patients and positive Toxoplasma serology.
[Mh] Termos MeSH primário: Infecções por HIV/complicações
Toxoplasmose Cerebral/complicações
Toxoplasmose Cerebral/tratamento farmacológico
Combinação Trimetoprima e Sulfametoxazol/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Antibacterianos/economia
Antibacterianos/uso terapêutico
Burkina Faso/epidemiologia
Estudos Transversais
Feminino
Infecções por HIV/epidemiologia
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Masculino
Meia-Idade
Fatores Socioeconômicos
Toxoplasmose Cerebral/sangue
Toxoplasmose Cerebral/epidemiologia
Combinação Trimetoprima e Sulfametoxazol/economia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 8064-90-2 (Trimethoprim, Sulfamethoxazole Drug Combination)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE
[do] DOI:10.17420/ap6303.103


  8 / 48726 MEDLINE  
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[PMID]:28453178
[Au] Autor:Nasrallah GK; Al Absi ES; Ghandour R; Ali NH; Taleb S; Hedaya L; Ali F; Huwaidy M; Husseini A
[Ad] Endereço:Department of Biomedical Sciences.
[Ti] Título:Seroprevalence of hepatitis E virus among blood donors in Qatar (2013-2016).
[So] Source:Transfusion;57(7):1801-1807, 2017 07.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hepatitis E virus (HEV) is an RNA virus transmitted mainly through zoonotic transmission or fecal-oral route. More than 80% of Qatar's population are expatriates, including many coming from hyperendemic countries; thus, it is important to estimate the seroprevalence and to compare between different nationalities. The results can be useful in alerting blood banks to the importance of HEV screening. STUDY DESIGN AND METHODS: Samples from 5854 blood donations provided by Hamad Medical Corporation were tested in the period between June 2013 to June 2016. Samples were tested for the presence of anti-HEV immunoglobulin (Ig)G and IgM antibodies and viral RNA using real-time polymerase chain reaction (PCR). Descriptive statistics, bivariate analysis, and multivariate logistic regression were used. RESULTS: Anti-HEV seroprevalence was 20.7%. A total of 1198 and 38 donations tested positive for IgG and IgM antibodies, respectively. Of the IgM-positive donations four tested positive by PCR. A significant association was detected between HEV seroprevalence with age and nationality. CONCLUSION: The seroprevalence of anti-HEV was high in Qatar. Since HEV IgM and RNA were detected, this suggests the possibility of HEV transmission by transfusion. Blood banks in Qatar and the region should consider screening for HEV, especially when transfusion is intended to pregnant women or immunocompromised patients.
[Mh] Termos MeSH primário: Doadores de Sangue
Anticorpos Anti-Hepatite/sangue
Vírus da Hepatite E/isolamento & purificação
[Mh] Termos MeSH secundário: Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Modelos Logísticos
Estudos Soroepidemiológicos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hepatitis Antibodies); 0 (Immunoglobulin G); 0 (Immunoglobulin M)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1111/trf.14116


  9 / 48726 MEDLINE  
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[PMID]:28742378
[Au] Autor:Arroyo G; Ortiz Barrientos KA; Lange K; Nave F; Miss Mas G; Lam Aguilar P; Soto Galindo MA
[Ad] Endereço:1 Department of Citohistología, Universidad de San Carlos de Guatemala , Guatemala, Guatemala .
[Ti] Título:Effect of the Various Steps in the Processing of Human Milk in the Concentrations of IgA, IgM, and Lactoferrin.
[So] Source:Breastfeed Med;12(7):443-445, 2017 09.
[Is] ISSN:1556-8342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Human milk immune components are unique and important for the development of the newborn. Milk processing at the Human Milk Banks (HMB), however, causes partial destruction of immune proteins. The objective of this study was to determine the effects that heating during the milk processing procedure at the HMB had on the concentrations of IgA, IgM, and lactoferrin at three critical points in time. MATERIALS AND METHODS: Fifty milk samples (150 mL) were collected from voluntary donors at the HMB at the Hospital Nacional Pedro de Bethancourt, located in Antigua Guatemala. Samples from three critical points in time during the milk processing procedure were selected for analysis: freezing/thawing I, freezing/thawing II, and pasteurization. IgA, IgM, and lactoferrin concentrations were determined during each critical point and compared with a baseline concentration. RESULTS: After milk processing, IgA, IgM, and lactoferrin mean concentrations were reduced by 30.0%, 36.0%, and 70.0%, respectively (p < 0.001). Reduction of biological activity was mainly attributed to pasteurization for IgA and lactoferrin (p < 0.001); the first freezing/thawing processes before pasteurization showed no significant reduction difference between mean concentrations of IgA (p = 0.160) and lactoferrin (p = 0.345) but showed a significant effect on IgM concentration (p = 0.016), and the second freezing/thawing procedure only showed a significant effect on IgA (p < 0.001). CONCLUSIONS: The effects of milk processing on the immune proteins that were evaluated in this study demonstrated a significant reduction.
[Mh] Termos MeSH primário: Conservação de Alimentos/métodos
Imunoglobulina A/análise
Imunoglobulina M/análise
Lactoferrina/análise
Bancos de Leite
Leite Humano/química
[Mh] Termos MeSH secundário: Feminino
Congelamento
Seres Humanos
Valor Nutritivo
Pasteurização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immunoglobulin A); 0 (Immunoglobulin M); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1089/bfm.2016.0154


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[PMID]:29241957
[Au] Autor:Luo YD; Zhang QL; Yao SJ; Lin DQ
[Ad] Endereço:Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
[Ti] Título:Evaluation of adsorption selectivity of immunoglobulins M, A and G and purification of immunoglobulin M with mixed-mode resins.
[So] Source:J Chromatogr A;1533:77-86, 2018 Jan 19.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study investigated adsorption selectivity of immunoglobulin M (IgM), immunoglobulin A (IgA) and immunoglobulin (IgG) on four mixed-mode resins with the functional ligands of 4-mercatoethyl-pyridine (MEP), 2-mercapto-1-methylimidazole (MMI), 5-aminobenzimidazole (ABI) and tryptophan-5-aminobenzimidazole (W-ABI), respectively. IgM purification processes with mixed-mode resins were also proposed. All resins showed typical pH-dependent adsorption, and high adsorption capacity was found at pH 5.0-8.0 with low adsorption capacity under acidic conditions. Meanwhile, high selectivity of IgM/IgA and IgM/IgG was obtained with ABI-4FF and MMI-4FF resins at pH 4.0-5.0, which was used to develop a method for IgM, IgA and IgG separation by controlling loading and elution pH. Capture of monoclonal IgM from cell culture supernatant with ABI-4FF resins was studied and high purity (∼99%) and good recovery (80.8%) were obtained. Moreover, IgM direct separation from human serum with combined two-step chromatography (ABI-4FF and MMI-4FF) was investigated, and IgM purity of 65.2% and a purification factor of 28.3 were obtained after optimization. The antibody activity of IgM was maintained after purification. The results demonstrated that mixed-mode chromatography with specially-designed ligands is a promising way to improve adsorption selectivity and process efficiency of IgM purification from complex feedstock.
[Mh] Termos MeSH primário: Técnicas de Química Analítica/métodos
Cromatografia
Imunoglobulina M/isolamento & purificação
Resinas Sintéticas/química
[Mh] Termos MeSH secundário: Adsorção
Células Cultivadas
Técnicas de Química Analítica/normas
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Imunoglobulina A/metabolismo
Imunoglobulina G/metabolismo
Imunoglobulina M/metabolismo
Ligantes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (Ligands); 0 (Resins, Synthetic)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE



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