Base de dados : MEDLINE
Pesquisa : D12.776.124.790 [Categoria DeCS]
Referências encontradas : 4197 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 420 ir para página                         

  1 / 4197 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:29267496
[Au] Autor:Wu L; Wang X; Chen F; Lv X; Sun W; Guo Y; Hou H; Ji H; Wei W; Gong L
[Ad] Endereço:Department of Ultrasonography, Tianjin Medical University General Hospital, Tianjin, China.
[Ti] Título:T cell subsets and immunoglobulin G levels are associated with the infection status of systemic lupus erythematosus patients.
[So] Source:Braz J Med Biol Res;51(2):e4547, 2017 Dec 11.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Systemic lupus erythematosus (SLE) is a chronic, autoimmune disorder that affects nearly all organs and tissues. As knowledge about the mechanism of SLE has increased, some immunosuppressive agents have become routinely used in clinical care, and infections have become one of the direct causes of mortality in SLE patients. To identify the risk factors indicative of infection in SLE patients, a case control study of our hospital's medical records between 2011 and 2013 was performed. We reviewed the records of 117 SLE patients with infection and 61 SLE patients without infection. Changes in the levels of T cell subsets, immunoglobulin G (IgG), complement C3, complement C4, globulin, and anti-double-stranded DNA (anti-ds-DNA) were detected. CD4+ and CD4+/CD8+ T cell levels were significantly lower and CD8+ T cell levels were significantly greater in SLE patients with infection than in SLE patients without infection. Additionally, the concentrations of IgG in SLE patients with infection were significantly lower than those in SLE patients without infection. However, complement C3, complement C4, globulin, and anti-ds-DNA levels were not significantly different in SLE patients with and without infection. Therefore, clinical testing for T cell subsets and IgG is potentially useful for identifying the presence of infection in SLE patients and for distinguishing a lupus flare from an acute infection.
[Mh] Termos MeSH primário: Imunoglobulina G/sangue
Infecção/sangue
Infecção/patologia
Lúpus Eritematoso Sistêmico/sangue
Lúpus Eritematoso Sistêmico/patologia
Subpopulações de Linfócitos T/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Anticorpos Antinucleares/sangue
Complemento C3/análise
Complemento C4/análise
Ensaio de Imunoadsorção Enzimática
Feminino
Citometria de Fluxo
Seres Humanos
Infecção/imunologia
Lúpus Eritematoso Sistêmico/imunologia
Masculino
Meia-Idade
Nefelometria e Turbidimetria
Reação em Cadeia da Polimerase
Fatores de Risco
Soroglobulinas/análise
Estatísticas não Paramétricas
Subpopulações de Linfócitos T/imunologia
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Antinuclear); 0 (Complement C3); 0 (Complement C4); 0 (Immunoglobulin G); 0 (Serum Globulins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


  2 / 4197 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28435998
[Au] Autor:Ganesh R; Suresh N; Vasanthi T; Sathiyasekaran M; Thulasiraman R
[Ad] Endereço:Kanchi Kamakoti CHILDS Trust Hospital and The CHILDS Trust Medical Research Foundation, 12-A, Nageswara Road, Nungambakkam, Chennai, 600 034, India. ganeped79@rediffmail.com.
[Ti] Título:A 6-year-old boy with Wilson disease-A diagnostic dilemma.
[So] Source:Indian J Gastroenterol;36(2):149-154, 2017 Mar.
[Is] ISSN:0975-0711
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:A 6-year-old boy presented with 2 months history of progressive abdominal distension and jaundice. He was deeply icteric with ascites, hepatosplenomegaly, hyperbilirubinemia, raised transaminases, and coagulopathy. Viral markers and slit lamp examination for Kayser-Fleischer ring were negative. Serum ceruloplasmin and 24-h urinary copper post-D-pencillamine challenge were normal. Anti-smooth muscle antibody was positive 1:20, and liver biopsy showed micronodular cirrhosis with abundant Mallory hyaline and stainable copper deposits. The liver histology was indicative of Indian childhood cirrhosis, whereas the presence of autoantibodies, elevated transaminases, and increased globulin was suggestive of autoimmune hepatitis. Gene studies identified p.R969Q mutation in ATP7B gene, which solved the dilemma and confirmed the diagnosis of Wilson disease (WD). We report a clinicopathological conference of this boy to highlight the challenges faced by pediatricians in the diagnosis of Wilson disease. á…Ÿ.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/genética
Proteínas de Transporte de Cátions/genética
Degeneração Hepatolenticular/diagnóstico
Degeneração Hepatolenticular/genética
Mutação
[Mh] Termos MeSH secundário: Autoanticorpos/sangue
Biomarcadores/sangue
Biomarcadores/metabolismo
Biópsia
Criança
Cobre/metabolismo
ATPases Transportadoras de Cobre
Testes Genéticos
Degeneração Hepatolenticular/patologia
Seres Humanos
Fígado/metabolismo
Fígado/patologia
Masculino
Músculo Liso/imunologia
Soroglobulinas/metabolismo
Transaminases/sangue
[Pt] Tipo de publicação:CASE REPORTS; CONGRESSES
[Nm] Nome de substância:
0 (Autoantibodies); 0 (Biomarkers); 0 (Cation Transport Proteins); 0 (Serum Globulins); 789U1901C5 (Copper); EC 2.6.1.- (Transaminases); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.3.54 (ATP7B protein, human); EC 3.6.3.54 (Copper-transporting ATPases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE
[do] DOI:10.1007/s12664-017-0746-4


  3 / 4197 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28369140
[Au] Autor:Yan T; Ke Y; Chen Y; Xu C; Yu C; Li Y
[Ad] Endereço:Department of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
[Ti] Título:Serological characteristics of autoimmune pancreatitis and its differential diagnosis from pancreatic cancer by using a combination of carbohydrate antigen 19-9, globulin, eosinophils and hemoglobin.
[So] Source:PLoS One;12(4):e0174735, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Autoimmune pancreatitis (AIP) is a special type of chronic pancreatitis, which may be misdiagnosed as pancreatic carcinoma. This study aims to verify new biomarkers for AIP and propose a serological pattern to differentiate AIP from pancreatic adenocarcinoma with routinely performed tests. In this study, data of serum samples were collected and compared between 25 patients with AIP and 100 patients with pancreatic carcinoma. Receiver operating characteristic analysis and logistic regression was performed to evaluate the diagnostic effect of serum parameters in differentiating AIP from pancreatic carcinoma alone or in combination. Among several serum markers observed in the two groups, carbohydrate antigen 19-9 (Ca19-9), globulin, eosinophils and hemoglobin were selected as the independent markers. Serum levels of Globulin, Eosinophil percentage in AIP group were significantly higher than in pancreatic cancer group (P<0.05), while hemoglobin and tumor marker CA19-9 levels were lower (P <0.05). The combination of these markers identified patients with AIP with 92% sensitivity and 79% specificity, which indicated relatively high diagnostic value. Elevated serum eosinophils, globulin, together with decreased hemoglobin level can be used as a preoperative indicator for AIP and can help to initiate diagnosis of AIP in time.
[Mh] Termos MeSH primário: Adenocarcinoma/diagnóstico
Doenças Autoimunes/diagnóstico
Antígeno CA-19-9/sangue
Eosinófilos/citologia
Hemoglobinas/análise
Neoplasias Pancreáticas/diagnóstico
Pancreatite/diagnóstico
[Mh] Termos MeSH secundário: Adenocarcinoma/sangue
Idoso
Doenças Autoimunes/sangue
Bilirrubina/sangue
Biomarcadores Tumorais/sangue
Estudos de Casos e Controles
Diagnóstico Diferencial
Feminino
Globulinas/análise
Seres Humanos
Contagem de Leucócitos
Fígado/enzimologia
Masculino
Meia-Idade
Neoplasias Pancreáticas/sangue
Pancreatite/sangue
Estudos Retrospectivos
Soroglobulinas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (CA-19-9 Antigen); 0 (Globulins); 0 (Hemoglobins); 0 (Serum Globulins); RFM9X3LJ49 (Bilirubin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174735


  4 / 4197 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28351295
[Au] Autor:Nagyová V; Arfuso F; Rizzo M; Nagy O; Piccione G
[Ad] Endereço:Clinic for Ruminants, University of Veterinary Medicine and Pharmacy in Kosice, Slovak Republic (Nagyová, Nagy).
[Ti] Título:Stability of ovine serum protein fractions under different storage conditions.
[So] Source:J Vet Diagn Invest;29(3):312-315, 2017 May.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We evaluated the effect of different storage conditions on the concentration of serum total proteins and their electrophoretic fractions in sheep. Blood samples were collected from 12 female Comisana sheep, and each serum obtained was divided into 4 aliquots. The first aliquot was analyzed immediately after separation (T0); the second was refrigerated at 4°C and analyzed after 24 h (T1), 48 h (T2), and 72 h (T3); the third was stored at -20°C immediately after centrifugation and analyzed after 2 days (T4); and the last aliquot was stored at -20°C after 24-h refrigeration and analyzed after 1 wk (T5). The concentrations of serum total proteins and their electrophoretic fractions (albumin, α-, ß1-, ß2-, and γ-globulins) was determined. A statistically significant ( p < 0.05) effect of storage conditions on all serum electrophoretic protein fractions and on the albumin-to-globulin (A/G) ratio was found. The values of albumin, α-globulins, and A/G ratio increased at T4 with respect to T0. The levels of α-globulins were higher at T1 than T0; the ß1-globulins levels changed at T3, whereas the ß2- and γ-globulins values as well as the A/G ratio changed at T2 and T3. At T4, we found an increase in the serum concentrations of albumin, α -globulins, and on the A/G ratio, and a decrease of γ fraction. At T5, the concentration of α-globulins was higher than at T0. Hence, we recommend electrophoretic analysis of fresh ovine serum samples or of samples refrigerated for no more than 24 h to obtain accurate data.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/análise
Albumina Sérica/análise
Soroglobulinas/análise
Ovinos/sangue
gama-Globulinas/análise
[Mh] Termos MeSH secundário: Animais
Análise Química do Sangue/veterinária
Estabilidade de Medicamentos
Eletroforese em Gel de Ágar/veterinária
Feminino
Albumina Sérica Humana
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (Serum Albumin); 0 (Serum Globulins); 0 (gamma-Globulins); 6D53G0FD0Z (plasma protein fraction); ZIF514RVZR (Serum Albumin, Human)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1177/1040638717700220


  5 / 4197 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27814784
[Au] Autor:Toiyama Y; Yasuda H; Ohi M; Yoshiyama S; Araki T; Tanaka K; Inoue Y; Mohri Y; Kusunoki M
[Ad] Endereço:Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Graduate School of Medicine, Mie University, Mie, Japan. Electronic address: ytoi0725@clin.medic.mie-u.ac.jp.
[Ti] Título:Clinical impact of preoperative albumin to globulin ratio in gastric cancer patients with curative intent.
[So] Source:Am J Surg;213(1):120-126, 2017 Jan.
[Is] ISSN:1879-1883
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Low albumin-to-globulin ratio (AGR) is associated with increased cancer mortality in patients with various cancers. However, there are no reports regarding the relation between pretreatment AGR and clinicopathological data, including oncological outcomes, in gastric cancer (GC) patients with curative intent. METHODS: Clinicopathological findings including preoperative laboratory data from 384 curative GC patients were assessed as indicators of early recurrence and poor prognosis in this retrospective study. AGR was calculated as albumin/(total protein - albumin)]. RESULTS: Several pathological categories related to tumor progression were associated with low AGR levels. Among preoperative serum markers, AGR was an independent predictor of early recurrence in curative GC patients. By multivariate analysis using both preoperative serum markers and postoperative clinicopathological categories, 2 pathological factors, lymph node metastasis and serosal invasion, and low AGR were independently associated with early recurrence. Furthermore, GC patients with low AGR showed significantly poor disease-free survival in the lymph node metastasis and serosal invasion groups. CONCLUSIONS: AGR was a novel independent predictor of early recurrence in curative GC patients.
[Mh] Termos MeSH primário: Albumina Sérica/metabolismo
Soroglobulinas/metabolismo
Neoplasias Gástricas/sangue
Neoplasias Gástricas/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Intervalo Livre de Doença
Feminino
Seres Humanos
Masculino
Meia-Idade
Valor Preditivo dos Testes
Período Pré-Operatório
Estudos Retrospectivos
Fatores de Risco
Neoplasias Gástricas/mortalidade
Taxa de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Serum Albumin); 0 (Serum Globulins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


  6 / 4197 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27705753
[Au] Autor:Franco M; Contreras C; Place NJ; Bozinovic F; Nespolo RF
[Ad] Endereço:Facultad de Ciencias Naturales y Matemáticas, Universidad de Ibagué, Carrera 22 Calle 67, Ibagué, Colombia.
[Ti] Título:Leptin levels, seasonality and thermal acclimation in the Microbiotherid marsupial Dromiciops gliroides: Does photoperiod play a role?
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;203:233-240, 2017 Jan.
[Is] ISSN:1531-4332
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mammals of the Neotropics are characterized by a marked annual cycle of activity, which is accompanied by several physiological changes at the levels of the whole organism, organs and tissues. The physiological characterization of these cycles is important, as it gives insight on the mechanisms by which animals adjust adaptively to seasonality. Here we studied the seasonal changes in blood biochemical parameters in the relict South American marsupial Dromiciops gliroides ("monito del monte" or "little mountain monkey"), under semi-natural conditions. We manipulated thermal conditions in order to characterize the effects of temperature and season on a battery of biochemical parameters, body mass and adiposity. Our results indicate that monitos experience an annual cycle in body mass and adiposity (measured as leptin levels), reaching a maximum in winter and a minimum in summer. Blood biochemistry confirms that the nutritional condition of animals is reduced in summer instead of winter (as generally reported). This was coincident with a reduction of several biochemical parameters in summer, such as betahydroxybutyrate, cholesterol, total protein concentration and globulins. Monitos seem to initiate winter preparation during autumn and reach maximum body reserves in winter. Hibernation lasts until spring, at which time they use fat reserves and become reproductively active. Sexual maturation during summer would be the strongest energetic bottleneck, which explains the reductions in body mass and other parameters in this season. Overall, this study suggests that monitos anticipate the cold season by a complex interaction of photoperiodic and thermal cues.
[Mh] Termos MeSH primário: Aclimatação
Adiposidade
Leptina/sangue
Marsupiais/fisiologia
Estado Nutricional
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico/sangue
Animais
Chile
Colesterol/sangue
Hibernação
Marsupiais/sangue
Marsupiais/crescimento & desenvolvimento
Fotoperíodo
Estações do Ano
Soroglobulinas/análise
Ganho de Peso
Perda de Peso
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Leptin); 0 (Serum Globulins); 97C5T2UQ7J (Cholesterol); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  7 / 4197 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28480357
[Au] Autor:A G; S D
[Ad] Endereço:Biochemistry Department, Veterinary Medical Faculty, Yuzuncu Yil University, Van, 65080, Turkey.
[Ti] Título:THE SERUM PROTEIN FRACTIONS IN THYMOQUINONE TREATED RATS.
[So] Source:Afr J Tradit Complement Altern Med;13(6):27-31, 2016.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: TQ has been used as treatment and preventive agent for many diseases over the years. The goal of this study was to investigate the effects of TQ supplement on fractions of serum proteins. MATERIALS AND METHODS: Fourteen male Wistar-Albino rats (200-250 g weight) were used as material for two groups; (control (C) and thymoquinone (TQ) respectively. Each group contained seven rats. The control group had only corn oil, while the TQ group was dissolved in corn oil. 30 mg/kg/day were given by oral gavage for four weeks. The serum protein fractions were identified using cellulose acetate technique. RESULTS: The total protein level and albumin, α-1, α-2 fractions and A/G ratio have showed no difference between groups (p>0.05). ß-globulin fractions of TQ group were higher than control's (p<0.05). In addition, it was observed that the γ-globulin levels of TQ group were lower than that of the control group's (p<0.05). CONCLUSION: From the results, it was observed that the changes of these fractions may have originated from elevation or decline synthesis, or activities of containing proteins.
[Mh] Termos MeSH primário: Benzoquinonas/farmacologia
Proteínas Sanguíneas/efeitos dos fármacos
Suplementos Nutricionais
Albumina Sérica/efeitos dos fármacos
Soroglobulinas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Masculino
Ratos
Ratos Wistar
Albumina Sérica Humana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoquinones); 0 (Blood Proteins); 0 (Serum Albumin); 0 (Serum Globulins); 490-91-5 (thymoquinone); 6D53G0FD0Z (plasma protein fraction); ZIF514RVZR (Serum Albumin, Human)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v13i6.6


  8 / 4197 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28080899
[Au] Autor:Knafo SE; Norton TM; Mitchell M; Stevenson DJ; Hyslop N; Poppenga R; Oliva M; Chen T; Cray C; Gibbs SE; Durden L; Stedman N; Divers S; Dierenfeld E
[Ti] Título:HEALTH AND NUTRITIONAL ASSESSMENT OF FREE-RANGING EASTERN INDIGO SNAKES (DRYMARCHON COUPERI) IN GEORGIA, UNITED STATES.
[So] Source:J Zoo Wildl Med;47(4):1000-1012, 2016 Dec.
[Is] ISSN:1042-7260
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Clinical pathology and nutritional parameters are useful in evaluating and monitoring threatened and endangered wildlife populations, but reference ranges for most snake species are lacking. From 2001 to 2005, health assessments were performed on 58 eastern indigo snakes (EIS) (Drymarchon couperi) captured in the wild in southeastern Georgia, United States. Health and nutritional assessments performed included hematology, serum biochemistry, fat-soluble vitamins, heavy metals, pesticide contaminants, parasitology, and surveys of other pathogens. Significant differences in total solids, packed cell volume, glucose, blood urea nitrogen, albumin : globulin ratio, amylase, triglycerides, and bile acids between males and females were observed. Additionally, there was a significant difference between liver and kidney concentrations for vitamins A and E. As previously noted in captive EIS, total Ca was elevated in comparison to concentrations reported in other snake species. Parasitism was a common finding in sampled EIS, but the overall health status of this free-ranging population appeared good. A winter-time dermatitis was found in most snakes, which resolved in the summer months. This study represents the first health and nutritional assessment of free-ranging EIS, and provides needed data to guide monitoring and conservation efforts.
[Mh] Termos MeSH primário: Distribuição Animal
Animais Selvagens
Estado Nutricional/fisiologia
Serpentes/fisiologia
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Aspartato Aminotransferases/sangue
Glicemia
Estudos Transversais
Eletrólitos/sangue
Feminino
Georgia
Masculino
Valores de Referência
Soroglobulinas
Serpentes/sangue
Ácido Úrico/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Electrolytes); 0 (Serum Globulins); 268B43MJ25 (Uric Acid); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1638/2015-0127.1


  9 / 4197 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27645784
[Au] Autor:Simmons M; Putnak R; Sun P; Burgess T; Marasco WA
[Ad] Endereço:Viral and Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland. monika.simmons.civ@mail.mil.
[Ti] Título:Antibody Prophylaxis Against Dengue Virus 2 Infection in Non-Human Primates.
[So] Source:Am J Trop Med Hyg;95(5):1148-1156, 2016 Nov 02.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Passive immunization with anti-dengue virus (DENV) immune serum globulin (ISG) or monoclonal antibodies (Mabs) may serve to supplement or replace vaccination for short-term dengue immune prophylaxis. In the present study, we sought to establish proof-of-concept by evaluating several DENV-neutralizing antibodies for their ability to protect rhesus macaques against viremia following live virus challenge, including human anti-dengue ISG, and a human Mab (Mab11/wt) and its genetically engineered variant (Mab11/mutFc) that is unable to bind to cells with Fc gamma receptors (FcγR) and potentiate antibody-dependent enhancement (ADE). In the first experiment, groups of animals received ISG or Mab11/wt at low doses (3-10 mg/kg) or a saline control followed by challenge with DENV-2 at day 10 or 30. After passive immunization, only low-titered circulating virus-neutralizing antibody titers were measured in both groups, which were undetectable by day 30. After challenge at day 10, a reduction in viremia duration compared with the control was seen only in the ISG group (75%). However, after a day 30 challenge, no reduction in viremia was observed in both immunized groups. In a second experiment to test the effect of higher antibody doses on short-term protection, groups received either ISG, Mab11/wt, Mab11/mutFc (each at 25 mg/kg) or saline followed by challenge with DENV-2 on day 10. Increased virus-neutralizing antibody titers were detected in all groups at day 5 postinjection, with geometric mean titers (GMTs) of 464 (ISG), 313 (Mab11/wt), and 309 (Mab11/mutFc). After challenge, there was complete protection against viremia in the group that received ISG, and a reduction in viremia duration of 89% and 83% in groups that received Mab11/wt and Mab11/mutFc, respectively. An in vitro ADE assay in Fcγ receptor-bearing K562 cells with sera collected immediately before challenge showed increased DENV-2 infection levels in the presence of both ISG and Mab11/wt, which peaked at a serum dilution of 1:90, but not in Mab11/mutFc containing sera. The results suggest that antibody prophylaxis for dengue might be beneficial in eliminating or reducing viral loads thereby minimizing disease progression. Our results also suggest that blocking FcγR interactions through Mab11 Fc engineering may further prevent ADE.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/uso terapêutico
Anticorpos Antivirais/sangue
Dengue/prevenção & controle
Imunização Passiva
Soroglobulinas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anticorpos Facilitadores
Dengue/sangue
Vírus da Dengue
Modelos Animais de Doenças
Seres Humanos
Imunoglobulina G/uso terapêutico
Células K562
Macaca mulatta/sangue
Macaca mulatta/imunologia
Macaca mulatta/virologia
Receptores de IgG/imunologia
Carga Viral
Viremia/sangue
Viremia/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Immunoglobulin G); 0 (Receptors, IgG); 0 (Serum Globulins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


  10 / 4197 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27430519
[Au] Autor:Shumyak S; Yang LJ; Han S; Zhuang H; Reeves WH
[Ad] Endereço:Division of Rheumatology & Clinical Immunology, University of Florida, Gainesville, FL 32610. United States.
[Ti] Título:"Lupoid hepatitis" in SLE patients and mice with experimental lupus.
[So] Source:Clin Immunol;172:65-71, 2016 Nov.
[Is] ISSN:1521-7035
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The unusual subset of patients with severe hepatitis, hypergammaglobulinemia, arthritis, and LE cells in the blood reported by Henry Kunkel and others suggested to these investigators that "lupoid" hepatitis might share pathogenic mechanisms with SLE. More than half a century later, the etiology of autoimmune hepatitis remains unclear. The occurrence of autoimmune hepatitis in a small fraction (about 3%) of SLE patients in our lupus cohort and in two mouse models of SLE supports their conclusion that lupoid hepatitis may be share pathogenic mechanisms with SLE. The development of autoimmune hepatitis in mice with pristane-induced lupus provides an opportunity to further explore the potential link between these two autoimmune disorders.
[Mh] Termos MeSH primário: Hepatite Autoimune
Lúpus Eritematoso Sistêmico
[Mh] Termos MeSH secundário: Actinas/imunologia
Adulto
Idoso
Animais
Autoanticorpos/sangue
Modelos Animais de Doenças
Feminino
Hepatite Autoimune/sangue
Hepatite Autoimune/imunologia
Hepatite Autoimune/patologia
Seres Humanos
Imunoglobulina G/sangue
Fígado/patologia
Lúpus Eritematoso Sistêmico/sangue
Lúpus Eritematoso Sistêmico/induzido quimicamente
Lúpus Eritematoso Sistêmico/imunologia
Lúpus Eritematoso Sistêmico/patologia
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Meia-Idade
Músculo Liso/imunologia
Soroglobulinas/análise
Terpenos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Autoantibodies); 0 (Immunoglobulin G); 0 (Serum Globulins); 0 (Terpenes); 26HZV48DT1 (pristane)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE



página 1 de 420 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde