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[PMID]:26522458
[Au] Autor:Pappa T; Ferrara AM; Refetoff S
[Ad] Endereço:The University of Chicago, MC3090, 5841 South Maryland Avenue, Chicago, IL 60637, USA. Electronic address: tpappa@medicine.bsd.uchicago.edu.
[Ti] Título:Inherited defects of thyroxine-binding proteins.
[So] Source:Best Pract Res Clin Endocrinol Metab;29(5):735-47, 2015 Oct.
[Is] ISSN:1878-1594
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Thyroid hormones (TH) are bound to three major serum transport proteins, thyroxine-binding globulin (TBG), transthyretin (TTR) and human serum albumin (HSA). TBG has the strongest affinity for TH, whereas HSA is the most abundant protein in plasma. Individuals harboring genetic variations in TH transport proteins present with altered thyroid function tests, but are clinically euthyroid and do not require treatment. Clinical awareness and early recognition of these conditions are important to prevent unnecessary therapy with possible untoward effects. This review summarizes the gene, molecular structure and properties of these TH transport proteins and provides an overview of their inherited abnormalities, clinical presentation, genetic background and pathophysiologic mechanisms.
[Mh] Termos MeSH primário: Hipertireoxinemia Disalbuminêmica Familiar/genética
Proteínas de Ligação a Tiroxina/genética
[Mh] Termos MeSH secundário: Seres Humanos
Hipertireoxinemia Disalbuminêmica Familiar/diagnóstico
Mutação
Hormônios Tireóideos/sangue
Hormônios Tireóideos/metabolismo
Proteínas de Ligação a Tiroxina/química
Proteínas de Ligação a Tiroxina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Thyroid Hormones); 0 (Thyroxine-Binding Proteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151103
[St] Status:MEDLINE


  2 / 2672 MEDLINE  
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[PMID]:25248730
[Au] Autor:Verkerk PH; van Trotsenburg AS; Hoorweg-Nijman JJ; Oostdijk W; van Tijn DA; Kempers MJ; van den Akker EL; Loeber JG; Elvers LH; Vulsma T
[Ad] Endereço:TNO, Child Health Department, Leiden.
[Ti] Título:[Neonatal screening for congenital hypothyroidism: more than 30 years of experience in the Netherlands].
[Ti] Título:Neonatale screening op congenitale hypothyreoïdie: ruim 30 jaar ervaring in Nederland..
[So] Source:Ned Tijdschr Geneeskd;158:A6564, 2014.
[Is] ISSN:1876-8784
[Cp] País de publicação:Netherlands
[La] Idioma:dut
[Ab] Resumo:OBJECTIVE: To describe the Dutch neonatal screening programme for congenital hypothyroidism (CH). DESIGN: Descriptive study. METHOD: Data on neonatal screening for CH in the period 1 January 1981 through 31 December 2011 were obtained from the Department for Vaccine Supply and Prevention Programmes of the Dutch National Institute for Public Health and the Environment (RIVM), laboratories and paediatricians to whom babies with abnormal screening results were referred. The screening procedure has been amended several times. In the period 1981-1994, only T4 and TSH were measured in heel prick blood, for example. From 1995, thyroxine-binding globulin (TBG) was added to the screening protocol. RESULTS: The participation rate was 99.7%. Before 1995 the sensitivity, specificity and positive predictive value were 94%, 99.51% and 6%, respectively. From 1995 these percentages were 98%, 99.85% and 21%, respectively. The total prevalence of CH was 1:2670 (prevalence of CH of thyroidal origin was 1:3100 and CH of central origin was 1:21,600). The percentages of patients with severe CH treated before day 15 in the periods 1981-1990, 1991-2000 and 2001-2011 were 24% (63/263), 63% (170/269) and 96% (176/184), respectively. CONCLUSION: The sensitivity and specificity of the screening procedure has considerably increased since 1995 compared with the period before 1995. In recent years patients with severe CH were treated considerably earlier than in the first years of the screening. Neonatal screening for CH may be considered as an important success for public health care.
[Mh] Termos MeSH primário: Hipotireoidismo Congênito/diagnóstico
Triagem Neonatal/métodos
[Mh] Termos MeSH secundário: Hipotireoidismo Congênito/sangue
Feminino
Seres Humanos
Recém-Nascido
Masculino
Triagem Neonatal/normas
Países Baixos/epidemiologia
Prevalência
Sensibilidade e Especificidade
Tireotropina/sangue
Tiroxina/sangue
Proteínas de Ligação a Tiroxina/análise
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Thyroxine-Binding Proteins); 9002-71-5 (Thyrotropin); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:140924
[Lr] Data última revisão:
140924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140925
[St] Status:MEDLINE


  3 / 2672 MEDLINE  
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[PMID]:25436710
[Au] Autor:Bílek R; Stárka L; Zamrazil V
[Ad] Endereço:Institute of Endocrinology, Národní 8, 116 94 Prague 1, Czech Republic. rbilek@endo.cz
[Ti] Título:Dysthyronemia in normal concentrations of thyrotropin--analytical and clinical consequences.
[So] Source:Horm Mol Biol Clin Investig;13(2):13-7, 2013 Jun.
[Is] ISSN:1868-1891
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This article discusses the conditions that may lead to a phenomenon called dysthyronemia. Here, the thyroid gland has concentration of thyrotropin in circulation within the reference range, but the concentrations of free or total fractions of thyroid hormones are outside the reference range. Normal values of thyrotropin (TSH) and increased values of THs are referred to as hyperthyroxinemia, while normal values of thyrotropin and decreased values of thyroid hormone are hypothyroxinemia. As shown by our observations, it is a relatively frequent situation in the parallel determinations of TSH and free thyroxine, when results verging on hyperthyroxinemia were found in 7% of cases (6.74%, n=259,590), and also in the parallel sets of TSH and total triiodothyronine when hypotriiodothyroninemia reached 8.5% (8.48%, n=73,143). We are assuming that the main cause of hyperthyroxinemia in the free thyroxine and TSH system is the presence of autoantibodies against thyroxine in patients with autoimmune thyroid disease. The reason of hypotriiodothyroninemia in the system of triiodothyronine and TSH is a decreased concentration of thyroid binding globulin in postmenopausal women. Manufacturers of immunoanalytical kits should take into account the potential adverse effects of autoantibodies against thyroid hormones when measuring the results of immunoassay determination of the free fraction of these hormones.
[Mh] Termos MeSH primário: Hipertireoxinemia/sangue
Hormônios Tireóideos/sangue
Tireotropina/sangue
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Hipertireoxinemia/diagnóstico
Masculino
Meia-Idade
Valores de Referência
Glândula Tireoide/metabolismo
Glândula Tireoide/patologia
Tiroxina/sangue
Proteínas de Ligação a Tiroxina/metabolismo
Tri-Iodotironina/sangue
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Thyroid Hormones); 0 (Thyroxine-Binding Proteins); 06LU7C9H1V (Triiodothyronine); 9002-71-5 (Thyrotropin); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:141202
[Lr] Data última revisão:
141202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141202
[St] Status:MEDLINE


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[PMID]:24086088
[Au] Autor:Roef G; Taes Y; Toye K; Goemaere S; Fiers T; Verstraete A; Kaufman JM
[Ad] Endereço:Departments of Endocrinology and Metabolic Bone Diseases.
[Ti] Título:Heredity and lifestyle in the determination of between-subject variation in thyroid hormone levels in euthyroid men.
[So] Source:Eur J Endocrinol;169(6):835-44, 2013 Dec.
[Is] ISSN:1479-683X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Variation in thyroid hormone (TH) concentrations between subjects is greater than in a single subject over a prolonged period of time, suggesting an individual set point for thyroid function. We have previously shown that TH levels within normal range are associated with clinical indices such as bone mass, BMI, and heart rate. The aim of this study on young men was therefore to gain insight into the determinants of variation in TH levels among healthy subjects. METHODS: Healthy male siblings (n=941, 25-45 years) were recruited in a cross-sectional, population-based study; a history or treatment of thyroid disease and thyroid auto-immunity were exclusion criteria. A complete assessment of TH status was performed (TSH, free thyroxine (FT4), free triiodothyronine (FT3), thyroperoxidase, and thyroglobulin antibodies, reverse T3 (rT3), thyroid-binding globulin (TBG), and urinary iodine levels). Genotyping was performed by TaqMan and KASP (KBiosciences) genotyping assays. RESULTS: (F)T4, rT3, and TBG had heritability estimates between 80 and 90%. Estimates were lower for (F)T3 (60%) and lowest for TSH (49%). Significant associations were observed between different single-nucleotide polymorphisms (SNPs) in the thyroid pathway and TSH, FT4, ratio FT3:FT4, and rT3. Nevertheless, these SNPs only explain a limited part of the heredity. As to age and lifestyle-related factors, (F)T3 was negatively related to age and education level, positively to smoking and BMI (all P<0.0001) but not substantially to urinary iodine concentrations. Smoking was also negatively related to TSH and positively to FT4. CONCLUSION: Both genetic and lifestyle-related factors play a role in determining between-subject variation in TH levels in euthyroid young men, although genetic factors seem most important.
[Mh] Termos MeSH primário: Hereditariedade
Estilo de Vida
Glândula Tireoide/metabolismo
Hormônios Tireóideos/sangue
[Mh] Termos MeSH secundário: Adulto
Fatores Etários
Bélgica
Estudos Transversais
Escolaridade
Genótipo
Seres Humanos
Iodeto Peroxidase/sangue
Masculino
Meia-Idade
Valores de Referência
Fumar/sangue
Tireoglobulina/sangue
Testes de Função Tireóidea
Tireotropina/sangue
Tiroxina/sangue
Proteínas de Ligação a Tiroxina/metabolismo
Tri-Iodotironina/sangue
Tri-Iodotironina Reversa/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Thyroid Hormones); 0 (Thyroxine-Binding Proteins); 06LU7C9H1V (Triiodothyronine); 5817-39-0 (Triiodothyronine, Reverse); 9002-71-5 (Thyrotropin); 9010-34-8 (Thyroglobulin); EC 1.11.1.8 (Iodide Peroxidase); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1312
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131003
[St] Status:MEDLINE
[do] DOI:10.1530/EJE-13-0265


  5 / 2672 MEDLINE  
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[PMID]:22956557
[Au] Autor:Roef G; Lapauw B; Goemaere S; Zmierczak HG; Toye K; Kaufman JM; Taes Y
[Ad] Endereço:Department of Endocrinology and Metabolic Bone Diseases, Ghent University Hospital, Belgium. greet.roef@ugent.be
[Ti] Título:Body composition and metabolic parameters are associated with variation in thyroid hormone levels among euthyroid young men.
[So] Source:Eur J Endocrinol;167(5):719-26, 2012 Nov.
[Is] ISSN:1479-683X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Thyroid disorders affect metabolism and body composition. Existing literature has been conflicting on whether this is also the case for thyroid hormone levels within the euthyroid range. Therefore, we have investigated the relationship between thyroid hormone concentrations and body composition together with metabolic parameters in a population of healthy euthyroid men. METHODS: Healthy male siblings (n=941, 25-45 years, median BMI 24.6) were recruited in a cross-sectional, population-based study; a history or treatment of thyroid disease and thyroid autoimmunity were exclusion criteria. Body composition and muscle cross-sectional area were assessed by dual-energy X-ray absorptiometry and peripheral quantitative computed tomography. Total (triiodothyronine (T(3); TT(3)) thyroxine and (T(4); TT(4))) and free thyroid hormones (FT(3) and FT(4)), TSH, and reverse T(3) (rT(3)) and thyroid-binding globulin (TBG) were determined using immunoassays. RESULTS: BMI was positively associated with (F)T(3) (P<0.0001). Whole body fat mass displayed positive associations with TT(3) and with (F)T(4) and TBG (P≤0.0006). Positive associations were further observed between leptin and (F)T(3), TT(4), and TBG (P≤0.0002). Inverse associations between lean mass and muscle cross-sectional area and (F)T(3), (F)T(4), and TBG were observed (P≤0.0003). Higher levels of (F)T(3) and TBG were associated with lower insulin sensitivity, assessed by homeostatic model assessment of insulin resistance (IR; P≤0.0001). No associations between TSH and body composition or metabolic parameters were seen. CONCLUSION: We show that a less favorable body composition (with higher fat and lower muscle mass and accompanying higher leptin concentrations) and IR are associated with higher thyroid hormone levels in healthy young men with well characterized euthyroidism.
[Mh] Termos MeSH primário: Tecido Adiposo
Composição Corporal
Índice de Massa Corporal
Resistência à Insulina
Leptina/sangue
Hormônios Tireóideos/sangue
[Mh] Termos MeSH secundário: Adulto
Biomarcadores/sangue
Proteínas de Transporte/sangue
Fatores de Confusão (Epidemiologia)
Estudos Transversais
Estradiol/sangue
Seres Humanos
Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina/metabolismo
Fator de Crescimento Insulin-Like I/metabolismo
Masculino
Proteínas de Membrana/sangue
Meia-Idade
Globulina de Ligação a Hormônio Sexual/metabolismo
Testosterona/sangue
Tireoglobulina/sangue
Tireotropina/sangue
Tiroxina/sangue
Proteínas de Ligação a Tiroxina/metabolismo
Tri-Iodotironina/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Carrier Proteins); 0 (IGFBP3 protein, human); 0 (Insulin-Like Growth Factor Binding Protein 3); 0 (Leptin); 0 (Membrane Proteins); 0 (Sex Hormone-Binding Globulin); 0 (Thyroid Hormones); 0 (Thyroxine-Binding Proteins); 0 (thyroid hormone-binding proteins); 06LU7C9H1V (Triiodothyronine); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); 67763-96-6 (Insulin-Like Growth Factor I); 9002-71-5 (Thyrotropin); 9010-34-8 (Thyroglobulin); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1212
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120908
[St] Status:MEDLINE
[do] DOI:10.1530/EJE-12-0447


  6 / 2672 MEDLINE  
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[PMID]:21910767
[Au] Autor:Lang MF; Salinin S; Ridder DA; Kleesiek J; Hroudova J; Berger S; Schütz G; Schwaninger M
[Ad] Endereço:Institute of Pharmacology, University of Heidelberg, Heidelberg, Germany.
[Ti] Título:A transgenic approach to identify thyroxine transporter-expressing structures in brain development.
[So] Source:J Neuroendocrinol;23(12):1194-203, 2011 Dec.
[Is] ISSN:1365-2826
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transporters are essential in thyroid hormone metabolism. Thyroxine (T4) is transported by solute carrier organic anion transporter 1c1 (SLCO1C1, OATP14) into the adult brain, where T4 is converted to 3,5,3'-triiodothyronine (T3). In adults, SLCO1C1 expression is found in two brain barrier structures: the blood-brain barrier (BBB) and choroid plexus. However, little is known about how T4 is transported in the developing brain, when the BBB is not yet completely formed. We employed bacterial artificial chromosome recombineering to generate transgenic mice carrying Cre recombinase in the Slco1c1 locus (Slco1c1-Cre mice). In Slco1c1-Cre mice Cre was expressed at the sites that have been previously reported for SLCO1C1 in adults. To trace Cre expression during development, we crossed Slco1c1-Cre transgenic mice with Rosa26 reporter mice. ß-galactosidase staining showed Cre activity in neurones of various brain structures, such as cortical layer 2/3 and the hippocampus, suggesting transient Slco1c1 expression during brain development. At embryonic day15, SLCO1C1 was expressed at the same site as TBR2, a marker of neuronal progenitors. Neurones that express SLCO1C1 during their development could be T4 sensitive. In support of this hypothesis, hypothyroxinaemia induced by propylthiouracil treatment of dams decreased the number of ß-galactosidase-positive neurones in cortical layer 2/3 of newborn Slco1c1-Cre/Rosa26 mice. In conclusion, by generating Slco1c1-Cre transgenic mice, we demonstrated that SLCO1C1 is expressed in the neuronal cell lineage during brain development. Expression of SLCO1C1 may underlie the extraordinary sensitivity of specific neuronal populations to hypothyroxinaemia.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Técnicas de Transferência de Genes
Proteínas de Transporte de Cátions Orgânicos/genética
Proteínas de Transporte de Cátions Orgânicos/metabolismo
[Mh] Termos MeSH secundário: Envelhecimento/genética
Envelhecimento/metabolismo
Animais
Encéfalo/embriologia
Encéfalo/crescimento & desenvolvimento
Células Cultivadas
Feminino
Hipotireoidismo/genética
Hipotireoidismo/metabolismo
Integrases/genética
Integrases/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Gravidez
Proteínas de Ligação a Tiroxina/genética
Proteínas de Ligação a Tiroxina/metabolismo
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Oatp2 protein, mouse); 0 (Organic Cation Transport Proteins); 0 (Thyroxine-Binding Proteins); EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:111118
[Lr] Data última revisão:
111118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110914
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-2826.2011.02216.x


  7 / 2672 MEDLINE  
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[PMID]:21885471
[Au] Autor:Wang Z; Lao HM; Liu T; Li LQ; Li M; Wu YS
[Ad] Endereço:Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
[Ti] Título:Labelled antibody-based one-step time-resolved fluoroimmunoassay for measurement of free thyroxine in serum.
[So] Source:Ann Clin Biochem;48(Pt 6):550-7, 2011 Nov.
[Is] ISSN:1758-1001
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Valid assays measuring free thyroxine (FT4) must perform without bias despite large variations in the concentrations and affinities of serum thyroxine-binding proteins in the population. We developed a new, rapid one-step labelled-antibody time-resolved fluoroimmunoassay (TRFIA) for FT4. METHODS: Based on the heterologous combination of anti-T4 monoclonal antibody and triiodothyronine-immunoglobulin G conjugate, a one-step TRFIA for FT4 detection was established and compared with the two-step DELFIA(®) Free Thyroxine Assay. Matrix interference caused by endogenous binders and exogenous non-esterified fatty acids (NEFA) was also accessed in the proposed assay. RESULTS: The developed method generally took only one hour, had a detection limit of 0.6 pmol/L and a large linear range of 2.5-120 pmol/L. The inter- and intra-assay coefficients of variation were 3.5-6.6% and 4.4-9.8%, respectively. Results from 110 specimens showed apparent agreement with that from the DELFIA(®) FT4 Assay with the square of the correlation coefficient of 0.975. This assay indicated that there was no significant dependence on endogenous binders and displayed potential interference by exogenous NEFA up to 5 mmol/L. CONCLUSIONS: The proposed one-step heterologous TRFIA FT4 assay possesses simplicity, accuracy, high sensitivity and exhibits great potential for FT4 measurement. The combination of heterologous immunoassay with TRFIA may be advantageous for FT4 immunoassay development.
[Mh] Termos MeSH primário: Tiroxina/sangue
[Mh] Termos MeSH secundário: Anticorpos Imobilizados
Calibragem
Estudos de Casos e Controles
Síndromes do Eutireóideo Doente/sangue
Síndromes do Eutireóideo Doente/diagnóstico
Ácidos Graxos não Esterificados/química
Técnica Direta de Fluorescência para Anticorpo/métodos
Técnica Direta de Fluorescência para Anticorpo/normas
Seres Humanos
Hipertireoidismo/sangue
Hipertireoidismo/diagnóstico
Hipotireoidismo/sangue
Hipotireoidismo/diagnóstico
Imunoglobulina G/química
Limite de Detecção
Padrões de Referência
Proteínas de Ligação a Tiroxina/química
Tri-Iodotironina/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Immobilized); 0 (Fatty Acids, Nonesterified); 0 (Immunoglobulin G); 0 (Thyroxine-Binding Proteins); 06LU7C9H1V (Triiodothyronine); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1202
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110903
[St] Status:MEDLINE
[do] DOI:10.1258/acb.2011.010242


  8 / 2672 MEDLINE  
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[PMID]:21842831
[Au] Autor:Simon E; Bytingsvik J; Jonker W; Leonards PE; de Boer J; Jenssen BM; Lie E; Aars J; Hamers T; Lamoree MH
[Ad] Endereço:Institute for Environmental Studies (IVM), VU University Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands.
[Ti] Título:Blood plasma sample preparation method for the assessment of thyroid hormone-disrupting potency in effect-directed analysis.
[So] Source:Environ Sci Technol;45(18):7936-44, 2011 Sep 15.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A sample preparation method combining solid-phase extraction (SPE) and liquid-liquid extraction (LLE) was developed to be used in Effect-Directed Analysis (EDA) of blood plasma. Until now such a method was not available. It can be used for extraction of a broad range of thyroid hormone (TH)-disruptors from plasma with high recoveries. Validation of the method using spiked cow plasma showed good recoveries for hydroxylated polybrominated diphenyl ethers (OH-PBDEs; 93.8 ± 19.5%), hydroxylated polychlorinated biphenyls (OH-PCBs; 93.8 ± 15.5%), other halogenated phenols (OHPs; 107 ± 8.1%), and for short-chain (<8 C-atoms) perfluoroalkyl substances (PFASs; 85.2 ± 24.6%). In the same extracts, the potency of the compound classes spiked to the cow plasma to competitively bind to transthyretin (TTR) was recovered by 84.9 ± 8.8%. Furthermore, the SPE-LLE method efficiently removed endogenous THs from the extracts, thereby eliminating their possible contribution to the binding assay response. The SPE-LLE method was applied to polar bear plasma samples to investigate its applicability in future EDA studies focusing on TH-disrupting compounds in this top predator species that is exposed to relatively high levels of bioaccumulating pollutants. A first screening revealed TTR-binding potency in the polar bear plasma extracts, which could be explained for 60-85% by the presence of OH-PCBs.
[Mh] Termos MeSH primário: Disruptores Endócrinos/metabolismo
Plasma/química
Proteínas de Ligação a Tiroxina/metabolismo
[Mh] Termos MeSH secundário: Animais
Ligação Competitiva
Bovinos/sangue
Disruptores Endócrinos/análise
Feminino
Fluorcarbonetos/análise
Fluorcarbonetos/metabolismo
Éteres Difenil Halogenados/análise
Éteres Difenil Halogenados/metabolismo
Fenóis/análise
Fenóis/metabolismo
Bifenilos Policlorados/análise
Bifenilos Policlorados/metabolismo
Reprodutibilidade dos Testes
Extração em Fase Sólida
Tiroxina/metabolismo
Ursidae/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Endocrine Disruptors); 0 (Fluorocarbons); 0 (Halogenated Diphenyl Ethers); 0 (Phenols); 0 (Thyroxine-Binding Proteins); DFC2HB4I0K (Polychlorinated Biphenyls); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110817
[St] Status:MEDLINE
[do] DOI:10.1021/es2016389


  9 / 2672 MEDLINE  
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[PMID]:21842493
[Au] Autor:Yang W; Shen S; Mu L; Yu H
[Ad] Endereço:Xuzhou Normal University, Jiangsu Key Laboratory of Green Synthetic Chemistry for Functional Materials, Xuzhou, Peoples Republic of China. yangwh70@126.com
[Ti] Título:Structure-activity relationship study on the binding of PBDEs with thyroxine transport proteins.
[So] Source:Environ Toxicol Chem;30(11):2431-9, 2011 Nov.
[Is] ISSN:1552-8618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Molecular docking and three-dimensional quantitative structure-activity relationships (3D-QSAR) were used to develop models to predict binding affinity of polybrominated diphenyl ether (PBDE) compounds to the human transthyretin (TTR). Based on the molecular conformations derived from the molecular docking, predictive comparative molecular similarity indices analysis (CoMSIA) models were developed. The results of CoMSIA models were as follows: leave-one-out (LOO) cross-validated squared coefficient q² (LOO) = 0.827 (full model, for all 28 compounds); q² (LOO) = 0.752 (split model, for 22 compounds in the training set); leave-many-out (LMO) cross-validated squared coefficient q² (LMO, two groups) = 0.723 ± 0.100 (full model, for all 28 compounds); q² (LMO, five groups) = 0.795 ± 0.030 (full model, for all 28 compounds); and the predictive squared correlation coefficient r²(pred) = 0.928 (for six compounds in the test set). The developed CoMSIA models can be used to infer the activities of compounds with similar structural characteristics. In addition, the interaction mechanism between hydroxylated polybrominated diphenyl ethers (HO-PBDEs) and the TTR was explored. Hydrogen bonding with amino acid residues Asp74, Ala29, and Asn27 may be an important determinant for HO-PBDEs binding to TTR. Among them, forming hydrogen bonds with amino acid residues Asp74 might exert a more important function.
[Mh] Termos MeSH primário: Éteres Difenil Halogenados/metabolismo
Modelos Químicos
Relação Quantitativa Estrutura-Atividade
Proteínas de Ligação a Tiroxina/metabolismo
[Mh] Termos MeSH secundário: Éteres Difenil Halogenados/química
Seres Humanos
Ligações de Hidrogênio
Conformação Molecular
Ligação Proteica
Proteínas de Ligação a Tiroxina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Halogenated Diphenyl Ethers); 0 (Thyroxine-Binding Proteins)
[Em] Mês de entrada:1201
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110816
[St] Status:MEDLINE
[do] DOI:10.1002/etc.645


  10 / 2672 MEDLINE  
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[PMID]:21722857
[Au] Autor:Tortosa F
[Ti] Título:[Subclinical thyroid dysfunction in pregnancy].
[Ti] Título:Disfunción tiroidea subclínica en la gestación..
[So] Source:Endocrinol Nutr;58(6):255-7, 2011 Jun-Jul.
[Is] ISSN:1579-2021
[Cp] País de publicação:Spain
[La] Idioma:spa
[Mh] Termos MeSH primário: Hipertireoidismo/diagnóstico
Hipotireoidismo/diagnóstico
Complicações na Gravidez/diagnóstico
Tireotropina/sangue
[Mh] Termos MeSH secundário: Animais
Estrogênios/fisiologia
Feminino
Seres Humanos
Hipertireoidismo/sangue
Hipertireoidismo/fisiopatologia
Hipotireoidismo/sangue
Hipotireoidismo/fisiopatologia
Absorção Intestinal
Iodo/deficiência
Iodo/farmacocinética
Tamanho do Órgão
Gravidez
Complicações na Gravidez/sangue
Complicações na Gravidez/fisiopatologia
Valores de Referência
Glândula Tireoide/fisiopatologia
Hormônios Tireóideos/sangue
Proteínas de Ligação a Tiroxina/análise
[Pt] Tipo de publicação:EDITORIAL
[Nm] Nome de substância:
0 (Estrogens); 0 (Thyroid Hormones); 0 (Thyroxine-Binding Proteins); 9002-71-5 (Thyrotropin); 9679TC07X4 (Iodine)
[Em] Mês de entrada:1111
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110705
[St] Status:MEDLINE
[do] DOI:10.1016/j.endonu.2011.05.001



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