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Pesquisa : D12.776.157.065 [Categoria DeCS]
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[PMID]:28542629
[Au] Autor:Ortega MS; Kurian JJ; McKenna R; Hansen PJ
[Ad] Endereço:Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program and Genetics Institute, University of Florida, Gainesville, Florida, United States of America.
[Ti] Título:Characteristics of candidate genes associated with embryonic development in the cow: Evidence for a role for WBP1 in development to the blastocyst stage.
[So] Source:PLoS One;12(5):e0178041, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The goal was to gain understanding of how 12 genes containing SNP previously related to embryo competence to become a blastocyst (BRINP3, C1QB, HSPA1L, IRF9, MON1B, PARM1, PCCB, PMM2, SLC18A2, TBC1D24, TTLL3 and WBP1) participate in embryonic development. Gene expression was evaluated in matured oocytes and embryos. BRINP3 and C1QB were not detected at any stage. For most other genes, transcript abundance declined as the embryo developed to the blastocyst stage. Exceptions were for PARM1 and WBP1, where steady-state mRNA increased at the 9-16 cell stage. The SNP in WBP1 caused large differences in the predicted three-dimensional structure of the protein while the SNP in PARM1 caused smaller changes. The mutation in WBP1 causes an amino acid substitution located close to a P-P-X-Y motif involved in protein-protein interactions. Moreover, the observation that the reference allele varies between mammalian species indicates that the locus has not been conserved during mammalian evolution. Knockdown of mRNA for WBP1 decreased the percent of putative zygotes becoming blastocysts and reduced the number of trophectoderm cells and immunoreactive CDX2 in the resulting blastocysts. WBP1 is an important gene for embryonic development in the cow. Further research to identify how the SNP in WBP1 affects processes leading to differentiation of the embryo into TE and ICM lineages is warranted.
[Mh] Termos MeSH primário: Blastocisto/metabolismo
Peptídeos e Proteínas de Sinalização Intracelular/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteína de Ligação a Androgênios/química
Proteína de Ligação a Androgênios/genética
Proteína de Ligação a Androgênios/metabolismo
Animais
Blastocisto/citologia
Bovinos
Células Cultivadas
Embrião de Mamíferos/metabolismo
Desenvolvimento Embrionário
Fertilização In Vitro
Regulação da Expressão Gênica no Desenvolvimento
Peptídeos e Proteínas de Sinalização Intracelular/antagonistas & inibidores
Peptídeos e Proteínas de Sinalização Intracelular/classificação
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
Oligonucleotídeos Antissenso/metabolismo
Oócitos/citologia
Oócitos/metabolismo
Filogenia
Polimorfismo de Nucleotídeo Único
Estrutura Terciária de Proteína
RNA Mensageiro/metabolismo
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 0 (Intracellular Signaling Peptides and Proteins); 0 (Oligonucleotides, Antisense); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178041


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[PMID]:28299766
[Au] Autor:Jin G; Liu J; Qin Q; Gao S; Zhang F; Ma Y; Ding C; Dong L; Yin H; Wang Y
[Ad] Endereço:Central Laboratory, Shanxi Provincial People's Hospital, Affiliate of Shanxi Medical University, Taiyuan, China.
[Ti] Título:Increased Level of c-kit in Semen of Infertile Patients with Varicocele.
[So] Source:Urol J;14(2):3023-3027, 2017 Mar 16.
[Is] ISSN:1735-546X
[Cp] País de publicação:Iran
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Varicocele is the most common risk factor for male infertility, however, not all males with varicocele experience infertility. In fact, most patients with varicocele have normal spermatogenesis. The molecular mechanism of varicocele-associated infertility is yet to be completely understood. The aim of this study is to assess the association of a number of fertility regulatory factors on varicocele associated infertility and to throw light on the mechanism of varicocele-associated infertility. MATERIALS AND METHODS: Semen from 30 infertile patients with varicocele and 30 fertile men with varicocele were collected. The concentrations of the following factors in seminal plasma were determined by ELISA: follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), androgen binding protein (ABP), transferrin (Trf), inhibin B (INHB) and stem cell factor (SCF). The expression level of c-kit in seminal precipitate of patients with varicocele was detected by real-time PCR. RESULTS: The concentrations of sexual hormones, FSH, LH and T, had no differences between infertile patients with varicocele and fertile men with varicocele (P > 0.05). Factors secreted by Sertoli cells, ABP, Trf, INHB andSCF, showed no significant differences between the two groups (P > 0.05). Interestingly, the expression of c-kit was significant higher in infertile patients with varicocele than that in fertile men with varicocele (P < 0.01). CONCLUSION: Neither the sexual hormones nor the Sertoli cells was responsible for the infertility induced by varicocele.The aberrant expression of c-kit in infertile patients with varicocele may provide new insight into the mechanism of varicocele-associated infertility.
[Mh] Termos MeSH primário: Infertilidade Masculina/genética
Infertilidade Masculina/metabolismo
Proteínas Proto-Oncogênicas c-kit/genética
Sêmen/metabolismo
Varicocele/genética
Varicocele/metabolismo
[Mh] Termos MeSH secundário: Adulto
Proteína de Ligação a Androgênios/metabolismo
Estudos de Casos e Controles
Hormônio Foliculoestimulante/metabolismo
Expressão Gênica
Seres Humanos
Infertilidade Masculina/etiologia
Inibinas/metabolismo
Hormônio Luteinizante/metabolismo
Masculino
Análise do Sêmen
Fator de Células-Tronco/metabolismo
Testosterona/metabolismo
Transferrina/metabolismo
Varicocele/complicações
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 0 (Stem Cell Factor); 0 (Transferrin); 0 (inhibin B); 3XMK78S47O (Testosterone); 57285-09-3 (Inhibins); 9002-67-9 (Luteinizing Hormone); 9002-68-0 (Follicle Stimulating Hormone); EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE


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[PMID]:28233584
[Au] Autor:Gao Y; Qin L; Yang Y; Dong X; Zhao Z; Zhang G; Zhao Z
[Ad] Endereço:Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, Jilin 136100, China; College of Animal Science and Technology, Jilin Agriculture University, Xin Cheng Street 2888, Changchun, Jilin 130118, China.
[Ti] Título:PDPN gene promotes the proliferation of immature Bovine Sertoli cells in vitro.
[So] Source:Anim Reprod Sci;179:35-43, 2017 Apr.
[Is] ISSN:1873-2232
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Podoplanin (PDPN) is a transmembrane receptor which is involved in various physiological and pathological processes, such as cell motility, invasion, tumor metastasis and blood vessels formation. Although there are reports on the involvement of PDPN in Sertoli cells in human and mice, the role of PDPN on the development of bovine Sertoli cells has not been reported. In the present study, Sertoli cells were isolated from 1-day-old bovine testes by two steps enzyme digestion method. Feulgen staining of satellite karyosomes and inhibin immunofluorescence staining suggested that the isolated immature Sertoli cells were very pure. Transfection with overexpression plasmid pBI-CMV3-PDPN and interference shRNA plasmid indicated that PDPN could significantly promote Sertoli cells cycle progression, cells proliferation and androgen-binding protein (ABP) production. Our results indicated that PDPN gene plays a significant role in the proliferation and maturation of bovine Sertoli cells.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Proliferação Celular/fisiologia
Regulação da Expressão Gênica/fisiologia
Glicoproteínas de Membrana/metabolismo
Células de Sertoli/fisiologia
[Mh] Termos MeSH secundário: Proteína de Ligação a Androgênios/genética
Proteína de Ligação a Androgênios/metabolismo
Animais
Células Cultivadas
Masculino
Glicoproteínas de Membrana/genética
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Regulação para Cima/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 0 (Membrane Glycoproteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE


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[PMID]:28159752
[Au] Autor:Chung AG; Belone PM; Bímová BV; Karn RC; Laukaitis CM
[Ad] Endereço:Department of Medicine, College of Medicine, University of Arizona, Tucson, Arizona 85724.
[Ti] Título:Studies of an Knockout Corroborate a Role for Salivary ABP in Mouse Communication.
[So] Source:Genetics;205(4):1517-1527, 2017 Apr.
[Is] ISSN:1943-2631
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The house mouse ( ) gene family is comprised of 64 paralogs, 30 and 34 , encoding the alpha (ABPA) and beta-gamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, and , by replacing them with the resistance gene. The knockout genotype (-/-) showed no or transcripts in submandibular gland complementary DNA (cDNA) libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the -/- genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands. We also observed a decrease in BG26 protein in the -/- animals, suggesting monomer instability. Overall, we observed no major phenotypic changes in the -/- genotype, compared with their +/+ and +/- siblings raised in a laboratory setting, including normal growth curves, tissue histology, fecundity, and longevity. The only difference is that male and female C57BL/6 mice preferred saliva of the opposite sex containing ABP statistically significantly more than saliva of the opposite sex without ABP in a Y-maze test. These results show for the first time that mice can sense the presence of ABP between saliva targets with and without ABPs, and that they spend more time investigating the target containing ABP.
[Mh] Termos MeSH primário: Proteína de Ligação a Androgênios/genética
Fenótipo
Glândulas Salivares/metabolismo
[Mh] Termos MeSH secundário: Proteína de Ligação a Androgênios/metabolismo
Animais
Feminino
Fertilidade
Longevidade
Masculino
Preferência de Acasalamento Animal
Aprendizagem em Labirinto
Camundongos
Proteoma
Saliva/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 0 (Proteome)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE
[do] DOI:10.1534/genetics.116.194571


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[PMID]:27444581
[Au] Autor:Heidargholizadeh S; Aydos SE; Yukselten Y; Ozkavukcu S; Sunguroglu A; Aydos K
[Ad] Endereço:Department of Medical Biology, School of Medicine, Ankara University, Ankara, Turkey.
[Ti] Título:A differential cytokine expression profile before and after rFSH treatment in Sertoli cell cultures of men with nonobstructive azoospermia.
[So] Source:Andrologia;49(4), 2017 May.
[Is] ISSN:1439-0272
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:To evaluate the effects of follicle-stimulating hormone (FSH) treatment on cytokine gene expression in cultured Sertoli cells from men with nonobstructive azoospermia, a total of 15 azoospermic men diagnosed as obstructive azoospermia (OA) (n = 5) and nonobstructive azoospermia (NOA) (n = 10) were included in the study. NOA patients were split into two further subgroups: nFSH and hFSH serum FSH levels. Expression of cytokine gene panel (88 genes), FSHR and ABP was evaluated by real-time PCR array analysis. FSHR protein level was measured by the Western blot. In primary cultures of Sertoli cells, seven genes were found to be increased and 13 were decreased in NOA group, when compared to OA (p < .05). When rFSH was introduced into the culture media, expression of 12 genes in the NOA group restored a comparable level to those of the control OA group. Sertoli cells in all groups responded rFSH administration with increased expression of ABP. Our results suggest that FSH treatment may have positive effects on Sertoli cells of nonobstructive azoospermic patients via changing the expression levels of certain genes and restoring their levels in normal Sertoli cell population. Some cytokine levels can be considered as a potential candidate for detecting NOA patients. ABP is a good marker for cell viability and functionality in primary Sertoli cell culture.
[Mh] Termos MeSH primário: Azoospermia/metabolismo
Citocinas/metabolismo
Hormônio Foliculoestimulante Humano/farmacologia
Células de Sertoli/efeitos dos fármacos
Espermatogênese
[Mh] Termos MeSH secundário: Proteína de Ligação a Androgênios/análise
Azoospermia/sangue
Sobrevivência Celular
Hormônio Foliculoestimulante Humano/sangue
Seres Humanos
Masculino
Cultura Primária de Células
Reação em Cadeia da Polimerase em Tempo Real
Receptores do FSH/análise
Proteínas Recombinantes/farmacologia
Células de Sertoli/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 0 (Cytokines); 0 (Follicle Stimulating Hormone, Human); 0 (Receptors, FSH); 0 (Recombinant Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE
[do] DOI:10.1111/and.12647


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[PMID]:27154732
[Au] Autor:Duan L; Zhu J; Wang K; Zhou G; Yang Y; Cui L; Huang H; Cheng X; Ba Y
[Ad] Endereço:Department of Occupational and Environmental Health, Institute of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China.
[Ti] Título:Does Fluoride Affect Serum Testosterone and Androgen Binding Protein with Age-Specificity? A Population-Based Cross-Sectional Study in Chinese Male Farmers.
[So] Source:Biol Trace Elem Res;174(2):294-299, 2016 Dec.
[Is] ISSN:1559-0720
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many studies have demonstrated that exposure to excess fluoride was associated with a variety of diseases. Little is known about the variation of testosterone (T) levels caused by fluoride exposure. The aim of this study is to explore the association of fluoride exposure and age with serum T and androgen-binding protein (ABP) levels in male farmers. A cross-sectional study was conducted in a county of Henan Province, China, including high fluoride exposure from drinking water villages and control villages. Male farmers aged 18-55 years old who lived in these villages were recruited by cluster sampling and divided into a higher fluoride exposure group (HFG) and a lower fluoride exposure group (LFG) according to the level of urinary fluoride. Levels of T and ABP in serum were measured using chemiluminescence immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) respectively. Markedly lower T levels were observed in male farmers from the HFG than in those from the LFG (t = 2.496, P < 0.05). Furthermore, younger farmers, 18-29 and 30-39 years old, may be the most likely to have lower T levels when exposed to fluoride (P < 0.05). No significant differences were observed in serum ABP levels in all male farmers between the two groups with different fluoride exposure. These results supported that excess fluoride exposure decreased serum T levels of male farmers with age-specificity.
[Mh] Termos MeSH primário: Envelhecimento/sangue
Agricultura
Proteína de Ligação a Androgênios/sangue
Fluoretos/sangue
Exposição Ocupacional
Testosterona/sangue
[Mh] Termos MeSH secundário: Adolescente
Adulto
China
Estudos Transversais
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 3XMK78S47O (Testosterone); Q80VPU408O (Fluorides)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160508
[St] Status:MEDLINE


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[PMID]:26851377
[Au] Autor:Zheng W; Pan S; Wang G; Wang YJ; Liu Q; Gu J; Yuan Y; Liu XZ; Liu ZP; Bian JC
[Ad] Endereço:College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, China.
[Ti] Título:Zearalenone impairs the male reproductive system functions via inducing structural and functional alterations of sertoli cells.
[So] Source:Environ Toxicol Pharmacol;42:146-55, 2016 Mar.
[Is] ISSN:1872-7077
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the effects of ZEA on the cytoskeletal structure, and factors specifically expressed by Sertoli cells. Primary Sertoli cells from rats aged 18-21 days were exposed to increasing ZEA concentrations (0, 5, 10, 20 µg mL(-1)) for 24 h. The results of immunofluorescence showed disruption of α-tubulin filaments and F-actin bundles, and damage to the nucleus of Sertoli cells on exposure to ZEA. In the control group, the protein level expression of androgen-binding protein (ABP), transferrin, vimentin, N-cadherin, and follicle-stimulating hormone receptor (FSHR) were decreased significantly (p<0.05, p<0.01). The mRNA levels of ABP, transferrin, vimentin, N-cadherin, and FSHR varied significantly in the experimental group (p<0.05). The results of enzyme-linked immunosorbent assay indicated a significant decrease in the levels of inhibin-ß and transferrin in the cultural supernatants (p<0.05). Additionally, the ultrastructural analysis indicated the absence of mitochondria and Golgi apparatus, and presence of vacuoles in the cytoplasm. These findings showed that ZEA treatment can damage the cytoskeletal structure and affect specific secretory functions of Sertoli cells, which may be an underlying cause of ZEA-induced reproductive toxicity.
[Mh] Termos MeSH primário: Estrogênios não Esteroides/toxicidade
Zearalenona/toxicidade
[Mh] Termos MeSH secundário: Proteína de Ligação a Androgênios/metabolismo
Animais
Caderinas/metabolismo
Inibinas/metabolismo
Masculino
RNA Mensageiro/metabolismo
Ratos
Receptores do FSH/metabolismo
Células de Sertoli/efeitos dos fármacos
Vimentina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 0 (Cadherins); 0 (Estrogens, Non-Steroidal); 0 (RNA, Messenger); 0 (Receptors, FSH); 0 (Vimentin); 0 (inhibin B); 57285-09-3 (Inhibins); 5W827M159J (Zearalenone)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160207
[St] Status:MEDLINE


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[PMID]:26039262
[Au] Autor:Chinta G; Ramya Chandar Charles M; Klopcic I; Sollner Dolenc M; Periyasamy L; Selvaraj Coumar M
[Ad] Endereço:Interdisciplinary Program in Life Sciences, Pondicherry University, Kalapet, Puducherry, India.
[Ti] Título:In Silico and In Vitro Investigation of the Piperine's Male Contraceptive Effect: Docking and Molecular Dynamics Simulation Studies in Androgen-Binding Protein and Androgen Receptor.
[So] Source:Planta Med;81(10):804-12, 2015 Jul.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Understanding the molecular mechanism of action of traditional medicines is an important step towards developing marketable drugs from them. Piperine, an active constituent present in the Piper species, is used extensively in Ayurvedic medicines (practiced on the Indian subcontinent). Among others, piperine is known to possess a male contraceptive effect; however, the molecular mechanism of action for this effect is not very clear. In this regard, detailed docking and molecular dynamics simulation studies of piperine with the androgen-binding protein and androgen receptors were carried out. Androgen receptors control male sexual behavior and fertility, while the androgen-binding protein binds testosterone and maintains its concentration at optimal levels to stimulate spermatogenesis in the testis. It was found that piperine docks to the androgen-binding protein, similar to dihydrotestosterone, and to androgen receptors, similar to cyproterone acetate (antagonist). Also, the piperine-androgen-binding protein and piperine-androgen receptors interactions were found to be stable throughout 30 ns of molecular dynamics simulation. Further, two independent simulations for 10 ns each also confirmed the stability of these interactions. Detailed analysis of the piperine-androgen-binding protein interactions shows that piperine interacts with Ser42 of the androgen-binding protein and could block the binding with its natural ligands dihydrotestosterone/testosterone. Moreover, piperine interacts with Thr577 of the androgen receptors in a manner similar to the antagonist cyproterone acetate. Based on the in silico results, piperine was tested in the MDA-kb2 cell line using the luciferase reporter gene assay and was found to antagonize the effect of dihydrotestosterone at nanomolar concentrations. Further detailed biochemical experiments could help to develop piperine as an effective male contraceptive agent in the future.
[Mh] Termos MeSH primário: Alcaloides/química
Alcaloides/farmacologia
Proteína de Ligação a Androgênios/metabolismo
Benzodioxóis/química
Benzodioxóis/farmacologia
Anticoncepcionais Masculinos/farmacologia
Piperidinas/química
Piperidinas/farmacologia
Alcamidas Poli-Insaturadas/química
Alcamidas Poli-Insaturadas/farmacologia
Receptores Androgênicos/metabolismo
[Mh] Termos MeSH secundário: Alcaloides/metabolismo
Proteína de Ligação a Androgênios/química
Benzodioxóis/metabolismo
Domínio Catalítico
Linhagem Celular/efeitos dos fármacos
Simulação por Computador
Anticoncepcionais Masculinos/química
Di-Hidrotestosterona/farmacologia
Seres Humanos
Ligações de Hidrogênio
Masculino
Metribolona/química
Metribolona/metabolismo
Metribolona/farmacologia
Simulação de Acoplamento Molecular
Simulação de Dinâmica Molecular
Piperidinas/metabolismo
Alcamidas Poli-Insaturadas/metabolismo
Conformação Proteica
Receptores Androgênicos/química
Serina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AR protein, human); 0 (Alkaloids); 0 (Androgen-Binding Protein); 0 (Benzodioxoles); 0 (Contraceptive Agents, Male); 0 (Piperidines); 0 (Polyunsaturated Alkamides); 0 (Receptors, Androgen); 08J2K08A3Y (Dihydrotestosterone); 2C323EGI97 (Metribolone); 452VLY9402 (Serine); U71XL721QK (piperine)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150710
[Lr] Data última revisão:
150710
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150604
[St] Status:MEDLINE
[do] DOI:10.1055/s-0035-1546082


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[PMID]:25745956
[Au] Autor:Ma Y; Yang HZ; Xu LM; Huang YR; Dai HL; Kang XN
[Ad] Endereço:1] Department of Biobank, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 1, 1630 DongFang Road, Shanghai. 200127, China [2] Department of Urology, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 7, 1630 DongFang Road, Shanghai. 200127, China.
[Ti] Título:Testosterone regulates the autophagic clearance of androgen binding protein in rat Sertoli cells.
[So] Source:Sci Rep;5:8894, 2015 Mar 09.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dysregulation of androgen-binding protein (ABP) is associated with a number of endocrine and andrology diseases. However, the ABP metabolism in Sertoli cells is largely unknown. We report that autophagy degrades ABP in rat Sertoli cells, and the autophagic clearance of ABP is regulated by testosterone, which prolongs the ABP biological half-life by inhibiting autophagy. Further studies identified that the autophagic clearance of ABP might be selectively regulated by testosterone, independent of stress (hypoxia)-induced autophagic degradation. These data demonstrate that testosterone up-regulates ABP expression at least partially by suppressing the autophagic degradation. We report a novel finding with respect to the mechanisms by which ABP is cleared, and by which the process is regulated in Sertoli cells.
[Mh] Termos MeSH primário: Proteína de Ligação a Androgênios/metabolismo
Autofagia/fisiologia
Células de Sertoli/citologia
Células de Sertoli/fisiologia
Testosterona/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Masculino
Taxa de Depuração Metabólica
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 3XMK78S47O (Testosterone)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150318
[Lr] Data última revisão:
150318
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150310
[St] Status:MEDLINE
[do] DOI:10.1038/srep08894


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[PMID]:25531410
[Au] Autor:Karn RC; Chung AG; Laukaitis CM
[Ad] Endereço:College of Medicine, University of Arizona, Tucson, Arizona, 85724, United States of America.
[Ti] Título:Did androgen-binding protein paralogs undergo neo- and/or Subfunctionalization as the Abp gene region expanded in the mouse genome?
[So] Source:PLoS One;9(12):e115454, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Androgen-binding protein (Abp) region of the mouse genome contains 30 Abpa genes encoding alpha subunits and 34 Abpbg genes encoding betagamma subunits, their products forming dimers composed of an alpha and a betagamma subunit. We endeavored to determine how many Abp genes are expressed as proteins in tears and saliva, and as transcripts in the exocrine glands producing them. Using standard PCR, we amplified Abp transcripts from cDNA libraries of C57BL/6 mice and found fifteen Abp gene transcripts in the lacrimal gland and five in the submandibular gland. Proteomic analyses identified proteins corresponding to eleven of the lacrimal gland transcripts, all of them different from the three salivary ABPs reported previously. Our qPCR results showed that five of the six transcripts that lacked corresponding proteins are expressed at very low levels compared to those transcripts with proteins. We found 1) no overlap in the repertoires of expressed Abp paralogs in lacrimal gland/tears and salivary glands/saliva; 2) substantial sex-limited expression of lacrimal gland/tear expressed-paralogs in males but no sex-limited expression in females; and 3) that the lacrimal gland/tear expressed-paralogs are found exclusively in ancestral clades 1, 2 and 3 of the five clades described previously while the salivary glands/saliva expressed-paralogs are found only in clade 5. The number of instances of extremely low levels of transcription without corresponding protein production in paralogs specific to tears and saliva suggested the role of subfunctionalization, a derived condition wherein genes that may have been expressed highly in both glands ancestrally were down-regulated subsequent to duplication. Thus, evidence for subfunctionalization can be seen in our data and we argue that the partitioning of paralog expression between lacrimal and salivary glands that we report here occurred as the result of adaptive evolution.
[Mh] Termos MeSH primário: Proteína de Ligação a Androgênios/genética
Proteína de Ligação a Androgênios/metabolismo
Genoma
Aparelho Lacrimal/metabolismo
Saliva/metabolismo
Glândula Submandibular/metabolismo
Lágrimas/metabolismo
[Mh] Termos MeSH secundário: Proteína de Ligação a Androgênios/classificação
Animais
Western Blotting
Células Cultivadas
Evolução Molecular
Feminino
Perfilação da Expressão Gênica
Aparelho Lacrimal/citologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Proteômica
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Saliva/citologia
Seleção Genética
Glândula Submandibular/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Androgen-Binding Protein); 0 (RNA, Messenger)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141223
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0115454



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