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Pesquisa : D12.776.157.125.090.500 [Categoria DeCS]
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[PMID]:28539338
[Au] Autor:Beggs MR; Appel I; Svenningsen P; Skjødt K; Alexander RT; Dimke H
[Ad] Endereço:Membrane Protein Disease Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, Canada.
[Ti] Título:Expression of transcellular and paracellular calcium and magnesium transport proteins in renal and intestinal epithelia during lactation.
[So] Source:Am J Physiol Renal Physiol;313(3):F629-F640, 2017 Sep 01.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Significant alterations in maternal calcium (Ca ) and magnesium (Mg ) balance occur during lactation. Ca is the primary divalent cation mobilized into breast milk by demineralization of the skeleton and alterations in intestinal and renal Ca transport. Mg is also concentrated in breast milk, but the underlying mechanisms are not well understood. To determine the molecular alterations in Ca and Mg transport in the intestine and kidney during lactation, three groups of female mice consisting of either nonpregnant controls, lactating mice, or mice undergoing involution were examined. The fractional excretion of Ca , but not Mg , rose significantly during lactation. Renal 1-α hydroxylase and 24-OHase mRNA levels increased markedly, as did plasma 1,25 dihydroxyvitamin D levels. This was accompanied by significant increases in intestinal expression of and in lactating mice. However, no alterations in the expression of cation-permeable claudin-2, claudin-12, or claudins-15 were found in the intestine. In the kidney, increased expression of and was observed during lactation, while no changes in claudins involved in Ca and Mg transport (claudin-2, claudin-14, claudin-16, or claudin-19) were found. Consistent with the mRNA expression, expression of both calbindin-D and transient receptor potential vanilloid 5 (TRPV5) proteins increased. Colonic expression increased during lactation, while renal remained unaltered. In conclusion, proteins involved in transcellular Ca and Mg transport pathways increase during lactation, while expression of paracellular transport proteins remained unchanged. Increased fractional Ca excretion can be explained by vitamin D-dependent intestinal hyperabsorption and bone demineralization, despite enhanced transcellular Ca uptake by the kidney.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Células Epiteliais/metabolismo
Mucosa Intestinal/metabolismo
Rim/metabolismo
Lactação/metabolismo
Magnésio/metabolismo
Glândulas Mamárias Animais/metabolismo
Proteínas de Membrana Transportadoras/metabolismo
[Mh] Termos MeSH secundário: 25-Hidroxivitamina D3 1-alfa-Hidroxilase/genética
25-Hidroxivitamina D3 1-alfa-Hidroxilase/metabolismo
Adaptação Fisiológica
Animais
Transporte Biológico
Calbindina 1/genética
Calbindina 1/metabolismo
Cálcio/urina
Canais de Cálcio/genética
Canais de Cálcio/metabolismo
Claudinas/genética
Claudinas/metabolismo
Feminino
Absorção Intestinal
Mucosa Intestinal/citologia
Rim/citologia
Proteínas de Membrana Transportadoras/genética
Camundongos
Reabsorção Renal
Proteína G de Ligação ao Cálcio S100/genética
Proteína G de Ligação ao Cálcio S100/metabolismo
Canais de Cátion TRPM/genética
Canais de Cátion TRPM/metabolismo
Canais de Cátion TRPV/genética
Canais de Cátion TRPV/metabolismo
Fatores de Tempo
Vitamina D/análogos & derivados
Vitamina D/sangue
Vitamina D3 24-Hidroxilase/genética
Vitamina D3 24-Hidroxilase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calb1 protein, mouse); 0 (Calbindin 1); 0 (Calcium Channels); 0 (Claudins); 0 (Membrane Transport Proteins); 0 (S100 Calcium Binding Protein G); 0 (S100g protein, mouse); 0 (TRPM Cation Channels); 0 (TRPV Cation Channels); 0 (Trpm6 protein, mouse); 0 (Trpv5 protein, mouse); 0 (Trpv6 protein, mouse); 1406-16-2 (Vitamin D); 66772-14-3 (1,25-dihydroxyvitamin D); EC 1.14.13.13 (25-Hydroxyvitamin D3 1-alpha-Hydroxylase); EC 1.14.15.16 (Cyp24a1 protein, mouse); EC 1.14.15.16 (Vitamin D3 24-Hydroxylase); I38ZP9992A (Magnesium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00680.2016


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[PMID]:27454846
[Au] Autor:Ishiguro K; Watanabe O; Nakamura M; Yamamura T; Ando T; Goto H; Hirooka Y
[Ad] Endereço:Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya, Aichi 466-8550, Japan. Electronic address: kio@med.nagoya-u.ac.jp.
[Ti] Título:S100G expression and function in fibroblasts on colitis induction.
[So] Source:Int Immunopharmacol;39:92-96, 2016 Oct.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Supplementation with interleukin (IL)-10, an important anti-inflammatory cytokine, has shown disappointing efficacy for inflammatory bowel diseases (IBD). IL-10 may down-regulate the expression of other anti-inflammatory mediators following colitis induction. We used a colitis model characterized by hapten-protein visualization, which indicates the site of hapten-protein formation after colitis induction for histological and gene expression analyses. Under IL-10 deficiency, following colitis induction inflammatory changes were reduced, and S100G expression was elevated. S100G was expressed in fibroblasts, and S100G expression was down-regulated by IL-10. S100G suppressed the production of monocyte chemotactic protein-1 (MCP-1) through the inhibition of NF-κB activation. Therefore, S100G, also known as Calbindin-D9k, may be an important anti-inflammatory mediator in fibroblasts following colitis induction, and down-regulation of S100G expression might be one reason for the insufficient performance of IL-10 supplementation.
[Mh] Termos MeSH primário: Quimiocina CCL2/metabolismo
Colite/metabolismo
Colo/patologia
Fibroblastos/metabolismo
Proteína G de Ligação ao Cálcio S100/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Modelos Animais de Doenças
Regulação da Expressão Gênica
Seres Humanos
Interleucina-10/genética
Interleucina-10/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
NF-kappa B/metabolismo
Proteína G de Ligação ao Cálcio S100/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokine CCL2); 0 (NF-kappa B); 0 (S100 Calcium Binding Protein G); 0 (S100G protein, human); 130068-27-8 (Interleukin-10)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170813
[Lr] Data última revisão:
170813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160726
[St] Status:MEDLINE


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[PMID]:27142230
[Au] Autor:Emam MA; Abouelroos ME; Gad FA
[Ad] Endereço:Histology and Cytology Dept., Faculty of Veterinary Medicine, Benha University, 13736, Egypt. Electronic address: mahmoud.hussein@fvtm.bu.edu.eg.
[Ti] Título:Expression of calbindin-D9k and vitamin D receptor in the uterus of Egyptian buffalo during follicular and luteal phases.
[So] Source:Acta Histochem;118(5):471-7, 2016 Jun.
[Is] ISSN:1618-0372
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Uteri of mature Egyptian buffalo cows (5-10 years old) were collected at follicular (n=12) and luteal (n=16) phases of estrous cycle to investigate the expression of calbindin-D9k (CaPB-9k) and vitamin D receptor (VDR). This study was done using avidin-biotin immunohistochemistry method. In addition, blood levels of calcium (Ca), vitamin D3 (Vit D), estrogen (E2) and progesterone (P4) were measured. The immunohistochemical findings restricted the expressions of CaBP-9k and VDR to the luminal and glandular epithelia of the endometrium implicating the importance of CaBP-9K and VDR in the function of endometrial epithelium, especially the glandular one, in order to prepare a receptive uterus. On the other hand, the myometrium did not express CaBP-9k or VDR that denies the potential role of CaBP-9k and VDR in the uterine contractility during the estrous cycle of Egyptian buffalo. All of Ca, Vit D, and P4 blood levels significantly (P<0.05) increased during luteal phase however, blood level of E2 significantly (P<0.05) increased during follicular phase. The expressions of CaBP-9k and VDR in the uterus of Egyptian buffalo were significantly (P<0.05) higher during luteal (P4 dominant) phase than during the follicular (E2 dominant) phase indicating that P4 up-regulates the expressions of CaBP-9k and VDR. In view of these observations, this study represents the first characterization of CaBP-9K and VDR expression in the uterus of Egyptian buffalo and suggests the pivotal role of CaBP-9k and VDR in the uterine receptivity. Furthermore, it demonstrates the regulatory role of P4 for expressions of CaBP-9k and VDR in buffalo uterus.
[Mh] Termos MeSH primário: Búfalos/fisiologia
Receptores de Calcitriol/metabolismo
Proteína G de Ligação ao Cálcio S100/metabolismo
Útero/metabolismo
[Mh] Termos MeSH secundário: Animais
Ciclo Estral
Feminino
Fase Folicular
Fase Luteal
Útero/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Calcitriol); 0 (S100 Calcium Binding Protein G)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170116
[Lr] Data última revisão:
170116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160505
[St] Status:MEDLINE


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[PMID]:26614488
[Au] Autor:Augustyniak R; Stanek J; Colaux H; Bodenhausen G; Kozminski W; Herrmann T; Ferrage F
[Ad] Endereço:Département de chimie, Ecole Normale Supérieure - PSL Research University, 24 rue Lhomond, 75005, Paris, France.
[Ti] Título:Nuclear overhauser spectroscopy of chiral CHD methylene groups.
[So] Source:J Biomol NMR;64(1):27-37, 2016 Jan.
[Is] ISSN:1573-5001
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nuclear magnetic resonance spectroscopy (NMR) can provide a great deal of information about structure and dynamics of biomolecules. The quality of an NMR structure strongly depends on the number of experimental observables and on their accurate conversion into geometric restraints. When distance restraints are derived from nuclear Overhauser effect spectroscopy (NOESY), stereo-specific assignments of prochiral atoms can contribute significantly to the accuracy of NMR structures of proteins and nucleic acids. Here we introduce a series of NOESY-based pulse sequences that can assist in the assignment of chiral CHD methylene protons in random fractionally deuterated proteins. Partial deuteration suppresses spin-diffusion between the two protons of CH2 groups that normally impedes the distinction of cross-relaxation networks for these two protons in NOESY spectra. Three and four-dimensional spectra allow one to distinguish cross-relaxation pathways involving either of the two methylene protons so that one can obtain stereospecific assignments. In addition, the analysis provides a large number of stereospecific distance restraints. Non-uniform sampling was used to ensure optimal signal resolution in 4D spectra and reduce ambiguities of the assignments. Automatic assignment procedures were modified for efficient and accurate stereospecific assignments during automated structure calculations based on 3D spectra. The protocol was applied to calcium-loaded calbindin D9k. A large number of stereospecific assignments lead to a significant improvement of the accuracy of the structure.
[Mh] Termos MeSH primário: Ressonância Magnética Nuclear Biomolecular/métodos
Proteínas/química
[Mh] Termos MeSH secundário: Modelos Moleculares
Conformação Molecular
Proteína G de Ligação ao Cálcio S100/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Proteins); 0 (S100 Calcium Binding Protein G)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:171021
[Lr] Data última revisão:
171021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151129
[St] Status:MEDLINE
[do] DOI:10.1007/s10858-015-0002-0


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[PMID]:26582761
[Au] Autor:Lee CT; Ng HY; Lee YT; Lai LW; Lien YH
[Ad] Endereço:Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang-Gung Memorial Hospital, Kaohsiung, Taiwan, and Chang-Gung University, College of Medicine, Taoyuan, Taiwan; Kidney Research Center, Chang Gung Memorial Hospital, Chang-Gung University, College of Medicine, Taoyuan, Taiwan;
[Ti] Título:The role of calbindin-D28k on renal calcium and magnesium handling during treatment with loop and thiazide diuretics.
[So] Source:Am J Physiol Renal Physiol;310(3):F230-6, 2016 Feb 01.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Calbindin-D28k (CBD-28k) is a calcium binding protein located in the distal convoluted tubule (DCT) and plays an important role in active calcium transport in the kidney. Loop and thiazide diuretics affect renal Ca and Mg handling: both cause Mg wasting, but have opposite effects on Ca excretion as loop diuretics increase, but thiazides decrease, Ca excretion. To understand the role of CBD-28k in renal Ca and Mg handling in response to diuretics treatment, we investigated renal Ca and Mg excretion and gene expression of DCT Ca and Mg transport molecules in wild-type (WT) and CBD-28k knockout (KO) mice. Mice were treated with chlorothiazide (CTZ; 50 mg · kg(-1) · day(-1)) or furosemide (FSM; 30 mg · kg(-1) · day(-1)) for 3 days. To avoid volume depletion, salt was supplemented in the drinking water. Urine Ca excretion was reduced in WT, but not in KO mice, by CTZ. FSM induced similar hypercalciuria in both groups. DCT Ca transport molecules, including transient receptor potential vanilloid 5 (TRPV5), TRPV6, and CBD-9k, were upregulated by CTZ and FSM in WT, but not in KO mice. Urine Mg excretion was increased and transient receptor potential subfamily M, member 6 (TRPM6) was upregulated by both CTZ and FSM in WT and KO mice. In conclusion, CBD-28k plays an important role in gene expression of DCT Ca, but not Mg, transport molecules, which may be related to its being a Ca, but not a Mg, intracellular sensor. The lack of upregulation of DCT Ca transport molecules by thiazides in the KO mice indicates that the DCT Ca transport system is critical for Ca conservation by thiazides.
[Mh] Termos MeSH primário: Calbindina 1/metabolismo
Cálcio/metabolismo
Clorotiazida/farmacologia
Furosemida/farmacologia
Túbulos Renais Distais/efeitos dos fármacos
Magnésio/metabolismo
Eliminação Renal/efeitos dos fármacos
Inibidores de Simportadores de Cloreto de Sódio/farmacologia
Inibidores de Simportadores de Cloreto de Sódio e Potássio/farmacologia
[Mh] Termos MeSH secundário: Animais
Western Blotting
Calbindina 1/deficiência
Calbindina 1/genética
Cálcio/urina
Canais de Cálcio/genética
Canais de Cálcio/metabolismo
Imunofluorescência
Regulação da Expressão Gênica
Genótipo
Túbulos Renais Distais/metabolismo
Magnésio/urina
Masculino
Camundongos Endogâmicos C57BL
Camundongos Knockout
Fenótipo
Reação em Cadeia da Polimerase em Tempo Real
Proteína G de Ligação ao Cálcio S100/genética
Proteína G de Ligação ao Cálcio S100/metabolismo
Canais de Cátion TRPV/genética
Canais de Cátion TRPV/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Calb1 protein, mouse); 0 (Calbindin 1); 0 (Calcium Channels); 0 (S100 Calcium Binding Protein G); 0 (S100g protein, mouse); 0 (Sodium Chloride Symporter Inhibitors); 0 (Sodium Potassium Chloride Symporter Inhibitors); 0 (TRPV Cation Channels); 0 (Trpv5 protein, mouse); 0 (Trpv6 protein, mouse); 77W477J15H (Chlorothiazide); 7LXU5N7ZO5 (Furosemide); I38ZP9992A (Magnesium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151120
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00057.2015


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[PMID]:26397986
[Au] Autor:Hess MW; de Baaij JH; Gommers LM; Hoenderop JG; Bindels RJ
[Ad] Endereço:Department of Physiology, Radboud Institute for Molecular Life Sciences, Radboud university medical center, Nijmegen, The Netherlands.
[Ti] Título:Dietary Inulin Fibers Prevent Proton-Pump Inhibitor (PPI)-Induced Hypocalcemia in Mice.
[So] Source:PLoS One;10(9):e0138881, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Proton-pump inhibitor-induced hypomagnesemia (PPIH) is the most recognized side effect of proton-pump inhibitors (PPIs). Additionally, PPIH is associated with hypocalcemia and hypokalemia. It is hypothesized that PPIs reduce epithelial proton secretion and thereby increase the pH in the colon, which may explain the reduced absorption of and Mg2+ and Ca2+. Fermentation of dietary oligofructose-enriched inulin fibers by the microflora leads to acidification of the intestinal lumen and by this enhances mineral uptake. This study aimed, therefore, to improve mineral absorption by application of dietary inulin to counteract PPIH. METHODS: Here, C57BL/J6 mice were supplemented with omeprazole and/or inulin. Subsequently, Mg2+ and Ca2+ homeostasis was assessed by means of serum, urine and fecal electrolyte measurements. Moreover, the mRNA levels of magnesiotropic and calciotropic genes were examined in the large intestine and kidney by real-time PCR. RESULTS: Treatment with omeprazole significantly reduced serum Mg2+ and Ca2+ levels. However, concomitant addition of dietary inulin fibers normalized serum Ca2+ but not serum Mg2+ concentrations. Inulin abolished enhanced expression of Trpv6 and S100g in the colon by omeprazole. Additionally, intestinal and renal mRNA levels of the Trpm6 gene were reduced after inulin intake. CONCLUSIONS: This study suggests that dietary inulin counteracts reduced intestinal Ca2+ absorption upon PPI treatment. In contrast, inulin did not increase intestinal absorption of Mg2+ sufficiently to recover serum Mg2+. The clinical potential of dietary inulin treatment should be the subject of future studies.
[Mh] Termos MeSH primário: Fibras na Dieta/administração & dosagem
Hipocalcemia/prevenção & controle
Inulina/administração & dosagem
Omeprazol/efeitos adversos
Inibidores da Bomba de Prótons/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Cálcio/sangue
Avaliação Pré-Clínica de Medicamentos
Ácidos Graxos/biossíntese
Hipocalcemia/sangue
Hipocalcemia/induzido quimicamente
Absorção Intestinal/efeitos dos fármacos
Magnésio/sangue
Masculino
Camundongos Endogâmicos C57BL
Proteína G de Ligação ao Cálcio S100/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dietary Fiber); 0 (Fatty Acids); 0 (Proton Pump Inhibitors); 0 (S100 Calcium Binding Protein G); 0 (S100g protein, mouse); 9005-80-5 (Inulin); I38ZP9992A (Magnesium); KG60484QX9 (Omeprazole); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150924
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0138881


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[PMID]:25880209
[Au] Autor:Herling TW; Arosio P; Müller T; Linse S; Knowles TP
[Ad] Endereço:Department of Chemistry, University of Cambridge, Cambridge, UK. tpjk2@cam.ac.uk.
[Ti] Título:A microfluidic platform for quantitative measurements of effective protein charges and single ion binding in solution.
[So] Source:Phys Chem Chem Phys;17(18):12161-7, 2015 May 14.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The charge state of proteins in solution is a key biophysical parameter that modulates both long and short range macromolecular interactions. However, unlike in the case of many small molecules, the effective charges of complex biomolecules in solution cannot in general be predicted reliably from their chemical structures alone. Here we present an approach for quantifying the effective charges of solvated biomolecules from independent measurements of their electrophoretic mobilities and diffusion coefficients in free solution within a microfluidic device. We illustrate the potential of this approach by determining the effective charges of a charge-ladder family of mutants of the calcium binding protein calbindin D9k in solution under native conditions. Furthermore, we explore ion-binding under native conditions, and demonstrate the ability to detect the chelation of a single calcium ion through the change that ion binding imparts on the effective charge of calbindin D9k. Our findings highlight the difference between the dry sequence charge and the effective charge of proteins in solution, and open up a route towards rapid and quantitative charge measurements in small volumes in the condensed phase.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Dispositivos Lab-On-A-Chip
Proteína G de Ligação ao Cálcio S100/química
Proteína G de Ligação ao Cálcio S100/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Desenho de Equipamento
Íons/metabolismo
Modelos Moleculares
Ligação Proteica
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ions); 0 (S100 Calcium Binding Protein G); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150430
[Lr] Data última revisão:
150430
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150417
[St] Status:MEDLINE
[do] DOI:10.1039/c5cp00746a


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[PMID]:25862956
[Au] Autor:Camacho L; Basavarajappa MS; Chang CW; Han T; Kobets T; Koturbash I; Surratt G; Lewis SM; Vanlandingham MM; Fuscoe JC; Gamboa da Costa G; Pogribny IP; Delclos KB
[Ad] Endereço:Division of Biochemical Toxicology, National Center for Toxicological Research, Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA. Electronic address: luisa.camacho@fda.hhs.gov.
[Ti] Título:Effects of oral exposure to bisphenol A on gene expression and global genomic DNA methylation in the prostate, female mammary gland, and uterus of NCTR Sprague-Dawley rats.
[So] Source:Food Chem Toxicol;81:92-103, 2015 Jul.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bisphenol A (BPA), an industrial chemical used in the manufacture of polycarbonate and epoxy resins, binds to the nuclear estrogen receptor with an affinity 4-5 orders of magnitude lower than that of estradiol. We reported previously that "high BPA" [100,000 and 300,000 µg/kg body weight (bw)/day], but not "low BPA" (2.5-2700 µg/kg bw/day), induced clear adverse effects in NCTR Sprague-Dawley rats gavaged daily from gestation day 6 through postnatal day (PND) 90. The "high BPA" effects partially overlapped those of ethinyl estradiol (EE2, 0.5 and 5.0 µg/kg bw/day). To evaluate further the potential of "low BPA" to induce biological effects, here we assessed the global genomic DNA methylation and gene expression in the prostate and female mammary glands, tissues identified previously as potential targets of BPA, and uterus, a sensitive estrogen-responsive tissue. Both doses of EE2 modulated gene expression, including of known estrogen-responsive genes, and PND 4 global gene expression data showed a partial overlap of the "high BPA" effects with those of EE2. The "low BPA" doses modulated the expression of several genes; however, the absence of a dose response reduces the likelihood that these changes were causally linked to the treatment. These results are consistent with the toxicity outcomes.
[Mh] Termos MeSH primário: Compostos Benzidrílicos/administração & dosagem
Compostos Benzidrílicos/toxicidade
Metilação de DNA/efeitos dos fármacos
Glândulas Mamárias Animais/efeitos dos fármacos
Fenóis/administração & dosagem
Fenóis/toxicidade
Próstata/efeitos dos fármacos
Útero/efeitos dos fármacos
[Mh] Termos MeSH secundário: Administração Oral
Animais
Cromatografia Líquida
Complemento C3/genética
Complemento C3/metabolismo
Relação Dose-Resposta a Droga
Etinilestradiol/administração & dosagem
Etinilestradiol/toxicidade
Feminino
Expressão Gênica
Genômica/métodos
Masculino
Glândulas Mamárias Animais/metabolismo
Metiltransferases/metabolismo
Gravidez
Efeitos Tardios da Exposição Pré-Natal/patologia
Próstata/metabolismo
Ligação Proteica
Ratos
Ratos Sprague-Dawley
Receptores Estrogênicos/genética
Receptores Estrogênicos/metabolismo
Receptores de Progesterona/genética
Receptores de Progesterona/metabolismo
Proteína G de Ligação ao Cálcio S100/genética
Proteína G de Ligação ao Cálcio S100/metabolismo
Espectrometria de Massas em Tandem
Útero/metabolismo
Fator A de Crescimento do Endotélio Vascular/genética
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Benzhydryl Compounds); 0 (Complement C3); 0 (Phenols); 0 (Receptors, Estrogen); 0 (Receptors, Progesterone); 0 (S100 Calcium Binding Protein G); 0 (Vascular Endothelial Growth Factor A); 0 (vascular endothelial growth factor A, rat); 423D2T571U (Ethinyl Estradiol); EC 2.1.1.- (Methyltransferases); MLT3645I99 (bisphenol A)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150412
[St] Status:MEDLINE


  9 / 4509 MEDLINE  
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[PMID]:25674214
[Au] Autor:Wang W; Knosp E; Tai G; Zhao Y; Liang Q; Guo Y
[Ad] Endereço:Department of Breast Surgery, China-Japan Union Hospital, Jilin University 126 Xiantai Blvd, Changchun 130033, P.R. China.
[Ti] Título:Differential effects of estrogen and estrogen receptor antagonist, ICI 182 780, on the expression of calbindin-D9k in rat pituitary prolactinoma GH3 cells.
[So] Source:Int J Clin Exp Pathol;7(12):8498-505, 2014.
[Is] ISSN:1936-2625
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To detect the effects of 17ß-estradiol (E2) on the expression of calbindin-D9k (CaBP-9k) in pituitary GH3 cells, and to determine the antagonistic effect of a selective estrogen receptor (ER) antagonist (ICI 182 780) on CaBP-9k expression. METHODS: A rat pituitary prolactinoma cell line (GH3 cells) was used in an in vitro model. The localization of CaBP-9k in GH3 cells was observed by immunofluorescence. GH3 cells were cultured with the addition of E2 medium for 24 hours. The levels of CaBP-9k mRNA and protein expression in different groups were analyzed by RT-PCR and Western blot analysis. The ER antagonist, ICI 182 780, was added to GH3 cells before E2 (10(-8) M) at a concentration of 10(-6) M to investigate the regulation of an ER-mediated pathway on CaBP-9k expression. RESULTS: E2 had a stimulatory effect on CaBP-9k expression of GH3 cells in a dose-dependent manner; the level of CaBP-9k expression was higher when treated with a higher concentration of E2. ICI 182 780 suppressed the stimulatory effect of E2 on CaBP-9k expression in GH3 cells. The level of CaBP-9k expression was significantly reduced by co-administration of E2 with ICI 182 780 in GH3 cells. The immunoprecipitation results confirmed that CaBP-9k interacts directly with ERα, and E2 increases the interaction between CaBP-9k and ERα. CONCLUSION: Estrogen induces CaBP-9k expression via an ERα-mediated pathway and CaBP-9k directly combines with ERα, suggesting that CaBP-9k is involved in the biological effects mediated by an ER pathway in GH3 cells.
[Mh] Termos MeSH primário: Estradiol/análogos & derivados
Estradiol/farmacologia
Antagonistas do Receptor de Estrogênio/farmacologia
Neoplasias Hipofisárias/metabolismo
Prolactinoma/metabolismo
Proteína G de Ligação ao Cálcio S100/biossíntese
[Mh] Termos MeSH secundário: Animais
Western Blotting
Linhagem Celular Tumoral
Imunofluorescência
Imunoprecipitação
Ratos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Estrogen Receptor Antagonists); 0 (S100 Calcium Binding Protein G); 22X328QOC4 (fulvestrant); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150214
[Lr] Data última revisão:
150214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150213
[St] Status:MEDLINE


  10 / 4509 MEDLINE  
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[PMID]:25251030
[Au] Autor:Kiyota Y; Takeda-Shitaka M
[Ad] Endereço:School of Pharmacy, Kitasato University , 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
[Ti] Título:Molecular recognition study on the binding of calcium to calbindin D9k based on 3D reference interaction site model theory.
[So] Source:J Phys Chem B;118(39):11496-503, 2014 Oct 02.
[Is] ISSN:1520-5207
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ca(2+)-binding proteins are widely distributed throughout cells and play various important roles. Calbindin D9k is a member of the EF-hand Ca(2+)-binding protein family. In this study, we examined the binding of Ca(2+) to calbindin D9k in terms of the free energy of solvation, as obtained by 3D reference interaction site model theory, which describes the statistical mechanics of liquids. We also investigated the main structural biological factor using spatial decomposition analysis in which the solvation free energy values are decomposed into the residue. We found some characteristic residues that contribute to stabilization of the holo-structure (Ca(2+)-binding structure). These results indicated that, in the holo-structure, these residues are newly exposed to solvent. Subsequently, the gain in solvation free energy, involving a conformational change and exposure to solvent, forms the driving force for binding of the Ca(2+) ion to the EF-hand.
[Mh] Termos MeSH primário: Cálcio/química
Modelos Teóricos
Proteína G de Ligação ao Cálcio S100/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Cálcio/metabolismo
Espectroscopia de Ressonância Magnética
Simulação de Dinâmica Molecular
Ligação Proteica
Estrutura Terciária de Proteína
Proteína G de Ligação ao Cálcio S100/metabolismo
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (S100 Calcium Binding Protein G); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:141003
[Lr] Data última revisão:
141003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140925
[St] Status:MEDLINE
[do] DOI:10.1021/jp504822r



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