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  1 / 11167 MEDLINE  
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[PMID]:29444082
[Au] Autor:Khan MI; Su YK; Zou J; Yang LW; Chou RH; Yu C
[Ad] Endereço:National Tsing Hua University, Chemistry Department, Hsinchu, Taiwan.
[Ti] Título:S100B as an antagonist to block the interaction between S100A1 and the RAGE V domain.
[So] Source:PLoS One;13(2):e0190545, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ca2+-binding human S100A1 protein is a type of S100 protein. S100A1 is a significant mediator during inflammation when Ca2+ binds to its EF-hand motifs. Receptors for advanced glycation end products (RAGE) correspond to 5 domains: the cytoplasmic, transmembrane, C2, C1, and V domains. The V domain of RAGE is one of the most important target proteins for S100A1. It binds to the hydrophobic surface and triggers signaling transduction cascades that induce cell growth, cell proliferation, and tumorigenesis. We used nuclear magnetic resonance (NMR) spectroscopy to characterize the interaction between S100A1 and the RAGE V domain. We found that S100B could interact with S100A1 via NMR 1H-15N HSQC titrations. We used the HADDOCK program to generate the following two binary complexes based on the NMR titration results: S100A1-RAGE V domain and S100A1-S100B. After overlapping these two complex structures, we found that S100B plays a crucial role in blocking the interaction site between RAGE V domain and S100A1. A cell proliferation assay WST-1 also supported our results. This report could potentially be useful for new protein development for cancer treatment.
[Mh] Termos MeSH primário: Antígenos de Neoplasias/metabolismo
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Subunidade beta da Proteína Ligante de Cálcio S100/fisiologia
Proteínas S100/metabolismo
[Mh] Termos MeSH secundário: Cálcio/metabolismo
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Ressonância Magnética Nuclear Biomolecular
Ligação Proteica
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Neoplasm); 0 (S100 Calcium Binding Protein beta Subunit); 0 (S100 Proteins); 0 (S100A1 protein); 0 (S100B protein, human); EC 2.7.11.22 (MOK protein, human); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190545


  2 / 11167 MEDLINE  
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[PMID]:29429162
[Au] Autor:Gong QX; Li H; Zhang ZH; Fan QH
[Ad] Endereço:Department of Pathology, the First Affiliated Hospital to Nanjing Medical University, Nanjing 210029, China.
[Ti] Título:[Pulmonary microcystic fibromyxoma: report of a case with review of literature].
[So] Source:Zhonghua Bing Li Xue Za Zhi;47(2):110-113, 2018 Feb 08.
[Is] ISSN:0529-5807
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To study the clinicopathologic features, diagnosis and differential diagnosis of pulmonary microcystic fibromyxoma. In March 2014, at the First Affiliated Hospital to Nanjing Medical University a 58-year-old female patient of pulmonary microcystic fibromyxoma was collected. The clinicopathologic, immunohistochemical and genetic profile of a case of pulmonary microcystic fibromyxoma were studied, and the relevant literature reviewed. The patient was a 58-year-old female who presented with cough and sputum for 1 month. CT scan disclosed a 15 mm nodule in her right middle lobe of lung. The patient underwent a wedge resection with negative margin. Grossly, a well-demarcated peripheral lung nodule was detected, measuring 1.5 cm×1.5 cm×1.0 cm, with myxoid tan-white cut surface containing microcysts. Microscopically, the tumor was composed of bland spindled to stellate-shaped cells widely spaced within prominent fibromyxoid stroma with prominent cystic change. No mitosis or necrosis was present. There were inconspicuous slim curvilinear capillaries and occasional collection of stromal lymphocytes and plasma cells. Immunohistochemically, the tumor cells were positive for vimentin, but negative for CD34, SMA, desmin, S-100 protein, ALK, CKpan, EMA, calretinin and TTF1. Fluorescence in situ hybridization did not show chromosomal translocation involving EWSR1, DDIT3 or FUS genes. The patient was recurrence or metastasis free after follow-up for 38 months. Pulmonary microcystic fibromyxoma is a rare benign lesion that should be differentiated from other lung tumors with myxoid characteristics.
[Mh] Termos MeSH primário: Fibroma/química
Fibroma/patologia
Neoplasias Pulmonares/química
Neoplasias Pulmonares/patologia
[Mh] Termos MeSH secundário: Proteínas de Ligação a Calmodulina/análise
Diagnóstico Diferencial
Feminino
Seres Humanos
Hibridização in Situ Fluorescente
Meia-Idade
Proteína EWS de Ligação a RNA/análise
Proteínas S100/análise
Tomografia Computadorizada por Raios X
Vimentina/análise
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Calmodulin-Binding Proteins); 0 (EWSR1 protein, human); 0 (RNA-Binding Protein EWS); 0 (S100 Proteins); 0 (Vimentin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-5807.2018.02.006


  3 / 11167 MEDLINE  
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[PMID]:29429160
[Au] Autor:Zhao M; Wang YB; Yan YJ; Wang W; Ru GQ; He XL
[Ad] Endereço:Department of Pathology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310014, China.
[Ti] Título:[Clinicopathologic features of atypical spindle cell lipomatous tumor].
[So] Source:Zhonghua Bing Li Xue Za Zhi;47(2):99-104, 2018 Feb 08.
[Is] ISSN:0529-5807
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the clinicopathologic characteristics, immunophenotype, differential and diagnostic features of atypical spindle cell lipomatous tumor (ASLT). Three cases of ASLT were collected from January 2010 to March 2017 at Zhejiang Provincial People's Hospital. The clinical and imaging features, histomorphology, immunophenotype and prognosis were analyzed. Fluorescence in situ hybridization (FISH) was used to detect MDM2 gene amplification, and relevant literature was reviewed. All three patients were adult males, aged 38, 43 and 54 years, respectively. One tumor originated in the subcutaneous soft tissue in the head and neck, one was located in the left primary bronchus and one in the latissimus dorsi muscle. Grossly, all three tumors were circumscribed and ranged from 4.0 to 5.8 cm in size. Microscopically, all showed a focally infiltrative front. These tumors were composed of variable proportions of spindle-shaped and adipocytic cells in a background of variable fibrous and edematous matrix. Scattered lipoblasts were easily seen. One tumor was composed predominately of spindle tumor cells, one of adipocytic cells, and one of equally mixed cell populations. The spindle tumor cells were generally bland-appearing with focal nuclear enlargement and hyperchromasia noted in one case. Mitosis was not seen in neither the spindle cells nor the adipocytic cells. By immunohistochemistry, diffuse and strong reactivity to CD34 of the spindle cells was noted in all cases, definite loss of Rb expression was noted in one of three cases, and S-100 protein was expressed only in the adipocytic cells. INI-1 was intact and Ki-67 index was 1% to 3%. All other markers including CDK4, MDM2, STAT6, SOX10, CD99, bcl-2, ß-catenin, CD117, GFAP, CK, EMA, SMA and desmin were negative. FISH of MDM2 was done in two cases, and both showed no amplification. The ASLT in the head and neck had two recurrences during 17 months of follow-up, whereas the tumor in the latissimus dorsi was free of disease during 33 months of follow-up. ASLT is a rare subtype of low-grade adipocytic neoplasm and is distinctive from atypical lipomatous tumor/well-differentiated liposarcoma. The histomorpholgy of ASLT has significant heterogeneity and forms a continuous spectrum. ASLT needs to be distinguished from a series of benign and malignant soft tissue tumors.
[Mh] Termos MeSH primário: Neoplasias Brônquicas/patologia
Neoplasias de Cabeça e Pescoço/patologia
Lipoma/patologia
Neoplasias Musculares/patologia
[Mh] Termos MeSH secundário: Adulto
Neoplasias Brônquicas/química
Neoplasias de Cabeça e Pescoço/química
Seres Humanos
Imuno-Histoquímica
Hibridização in Situ Fluorescente
Lipoma/química
Lipossarcoma/química
Lipossarcoma/patologia
Masculino
Meia-Idade
Neoplasias Musculares/química
Recidiva Local de Neoplasia
Proteínas S100/análise
Fator de Transcrição STAT6/análise
Músculos Superficiais do Dorso
beta Catenina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CTNNB1 protein, human); 0 (S100 Proteins); 0 (STAT6 Transcription Factor); 0 (STAT6 protein, human); 0 (beta Catenin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-5807.2018.02.004


  4 / 11167 MEDLINE  
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[PMID]:29443763
[Au] Autor:Zhang Z; Yang Z; Pan Q; Qin X; Deng Y; Cao Y
[Ad] Endereço:Jinhua Eye Hospital, Jinhua, ZheJiang.
[Ti] Título:Epibulbar complex cartilaginous choristoma: A distinctive clinicopathological case series and literature review.
[So] Source:Medicine (Baltimore);97(7):e9902, 2018 Feb.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To newly describe the clinical and histopathological characteristics of epibulbar complex cartilaginous choristoma incidentally observed in a series of pterygium excision patients.Noncomparative case series with chart review of 8 patients.During a 4-year period, we identified 8 cases of conventional unilateral nasal subpterygial cartilaginous choristoma in 1799 pterygium patients and analyzed their clinicopathological features. The incidence rate of this entity is 0.44% in pterygium patients. All of the cartilaginous choristomas were buried deep in the caruncle, covered by the pterygium, and embedded in tenon facia tissue. Its clinicopathological characteristics include hyaline cartilaginous tissue that is surrounded by fibrous connective tissue and smooth muscle bundles. S-100 protein-staining specifically revealed chondrocytes embedded within chondroid matrix.Epibulbar complex cartilaginous choristoma covered by pterygia and predominantly observed in the older population is rare. The lesions were buried deep in the caruncle, covered by the pterygium and embedded in tenon fascia tissue. These findings are inconsistent with those in previous reports.
[Mh] Termos MeSH primário: Coristoma
Túnica Conjuntiva
Procedimentos Cirúrgicos Oftalmológicos
Pterígio
[Mh] Termos MeSH secundário: Fatores Etários
China/epidemiologia
Coristoma/epidemiologia
Coristoma/patologia
Coristoma/cirurgia
Feminino
Seres Humanos
Cartilagem Hialina/patologia
Achados Incidentais
Masculino
Meia-Idade
Procedimentos Cirúrgicos Oftalmológicos/métodos
Procedimentos Cirúrgicos Oftalmológicos/estatística & dados numéricos
Pterígio/epidemiologia
Pterígio/patologia
Pterígio/cirurgia
Fatores de Risco
Proteínas S100/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (S100 Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009902


  5 / 11167 MEDLINE  
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[PMID]:29443751
[Au] Autor:Zhang K; Qu S; Li J; Cheng Y; Shi J; Liu T
[Ad] Endereço:Department of General Surgery.
[Ti] Título:A case report of rectal schwannoma treated with laparoscopic proctectomy.
[So] Source:Medicine (Baltimore);97(7):e9866, 2018 Feb.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Schwannomas of gastrointestinal tracts are rare and difficult to detect preoperatively because of negative results of endoscopic and imaging examinations. Here, we reported a case of rectal schwannoma, which was diagnosed by immunohistochemical staining after laparoscopic protectomy. PATIENT CONCERNS: A 61-year-old woman complained of a 1-month history of difficulty in defecation and irregularly abdominal discomfort during her physical checkup in our hospital. DIAGNOSES: Immunohistochemical staining results after laparoscopic protectomy revealed a strong positive reaction for S-100 protein. Therefore, rectal schwannoma was confirmed. INTERVENTIONS: Treatment with laparoscopic protectomy was given. OUTCOMES: Symptoms resolved completely after 12 days of the surgery, and was regular followed-up in outpatient clinic. LESSONS: Schwannomas are difficult to identify preoperatively, and immunohistochemical staining for S-100 protein is an effective method to diagnose it.
[Mh] Termos MeSH primário: Procedimentos Cirúrgicos do Sistema Digestório/métodos
Laparoscopia/métodos
Neurilemoma
Neoplasias Retais
Proteínas S100/análise
[Mh] Termos MeSH secundário: Biópsia/métodos
Feminino
Seres Humanos
Imuno-Histoquímica
Meia-Idade
Neurilemoma/patologia
Neurilemoma/fisiopatologia
Neurilemoma/cirurgia
Neoplasias Retais/patologia
Neoplasias Retais/fisiopatologia
Neoplasias Retais/cirurgia
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (S100 Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009866


  6 / 11167 MEDLINE  
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[PMID]:29281699
[Au] Autor:Kim M; Yoo J; Kim J; Park J; Han E; Jang W; Chae H; Lee JH; Park YM; Kim Y
[Ad] Endereço:Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
[Ti] Título:Association of FLG single nucleotide variations with clinical phenotypes of atopic dermatitis.
[So] Source:PLoS One;12(12):e0190077, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: FLG encodes a large protein called profilaggrin, which plays a key role in maintaining an effective skin barrier against the environment. In this study, we identified FLG single nucleotide variations (FLG-SNVs) and evaluated the association of FLG-SNVs with clinical phenotypes including atopic dermatitis (AD)-associated minor clinical features, presence of specific allergic sensitization, and serum parameters. METHODS: Eighty-one Korean patients with AD were enrolled. AD-associated minor clinical features as well as allergic rhinitis and asthma were diagnosed by specialists. FLG-SNVs were identified by Sanger sequencing of entire exons through long-range PCR. Allergic sensitization to a specific allergen was evaluated by multiple allergen simultaneous test. Serologic parameters such as serum eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN) were measured. RESULTS: A total of seventy-three SNVs and 4 LOF mutations were successfully genotyped. rs71626704 and rs76413899 were significantly associated with a history of asthma and cheilitis (P = 0.002 and P = 0.033, respectively), however, the associations were not found statistically significant after adjustment by multiple comparisons. In addition, we detected haplotype blocks which were correlated with non-specific hand or foot dermatitis and scalp scale. We identified FLG-SNVs which were associated with sensitization to environmental allergens; rs62623409 and rs71625199 (P = 0.038 and P = 0.008, respectively). Patients with FLG P478S TT and history of allergic rhinitis showed a higher EDN level, and among those patients, the ones with asthma showed a higher ECP level. CONCLUSION: This study revealed the association of FLG-SNVs with AD-associated minor clinical features. We firstly identified rs71625199 which was associated with higher environmental allergic sensitization. We also suggest that FLG P478S is a kind of disease modifier which affects serologic parameters such as EDN and ECP.
[Mh] Termos MeSH primário: Dermatite Atópica/genética
Variação Genética
Proteínas S100/genética
[Mh] Termos MeSH secundário: Seres Humanos
Mutação
Fenótipo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (S100 Proteins); 0 (filaggrin-2 protein, human)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190077


  7 / 11167 MEDLINE  
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[PMID]:29325250
[Au] Autor:Fu Y; Guan WY; Wu HY; Wu HY; Fan ZW; Ye Q; Meng FQ
[Ad] Endereço:Department of Pathology, Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
[Ti] Título:[Myofibroma/myofibromatosis: a clinicopathologic analysis of 9 cases].
[So] Source:Zhonghua Bing Li Xue Za Zhi;47(1):45-50, 2018 Jan 08.
[Is] ISSN:0529-5807
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the clinical and histological features, diagnosis and differential diagnosis of myofibroma/myofibromatosis. The clinical data and pathology features of nine cases of myofibroma/myofibromatosis were collected from August 2011 to November 2016 in Affiliated Drum Tower Hospital, Nanjing University Medical School and Children's Hospital of Nanjing Medical University. Immunohistochemistry(IHC), PDGFRB molecular analysis and ETV6-NTRK3 gene fusion were performed and relevant literature reviewed. There were 7 males and 2 females, with age ranging from 3 days to 18 years (mean 5 years). The tumors were located in head and neck (eight cases) and trunk (one case). Clinically, the tumors presented as freely movable nodules. Microscopically, they appeared biphasic with alternating light- and dark-staining areas. The light-staining area consisted mainly of plump myoid spindle cells with eosinophilic cytoplasm arranged in nodules, short fascicles, or whorls.The dark-staining area was composed of round or polygonal cells with slightly hyperchromatic nuclei or small spindle cells arranged around a distinct hemangiopericytoma-like vascular pattern. IHC showed the tumor cells in the light-staining area were strongly positive for vimentin and SMA, while cells in dark-staining area were strongly positive for vimentin, and weakly for SMA. Tumor cells were negative for desmin, S-100 protein, h-Caldesmon, CD34 and STAT6. Analysis of PDGFRB mutations was performed in seven cases. Two cases showed 12 exon point mutation c. 1681 c>T(p.R561C), one case showed 14 exon point mutation c. 1998C>G (p.N666K). ETV6-NTRK3 gene fusion was not detected by fluorescence in situ hybridization in four patients under three years old. All cases were followed for 6 to 68 months, with two recurrences. Myofibroma/myofibromatosis is an uncommon benign myofibroblastic tumor of infancy and childhood. The tumor can appear biphasic, and may show PDGFRB point mutation which is of potential diagnostic value.
[Mh] Termos MeSH primário: Miofibroma
Miofibromatose
[Mh] Termos MeSH secundário: Adolescente
Antígenos CD34/análise
Proteínas de Ligação a Calmodulina/análise
Criança
Pré-Escolar
Desmina/análise
Diagnóstico Diferencial
Éxons
Feminino
Hemangiopericitoma/irrigação sanguínea
Seres Humanos
Imuno-Histoquímica
Hibridização in Situ Fluorescente
Masculino
Mutação
Miofibroma/diagnóstico
Miofibroma/genética
Miofibroma/patologia
Miofibromatose/diagnóstico
Miofibromatose/genética
Miofibromatose/patologia
Receptor beta de Fator de Crescimento Derivado de Plaquetas/análise
Receptor beta de Fator de Crescimento Derivado de Plaquetas/genética
Proteínas S100/análise
Fator de Transcrição STAT6/análise
Vimentina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD34); 0 (Calmodulin-Binding Proteins); 0 (Desmin); 0 (S100 Proteins); 0 (STAT6 Transcription Factor); 0 (STAT6 protein, human); 0 (Vimentin); EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-5807.2018.01.009


  8 / 11167 MEDLINE  
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[PMID]:29224278
[Au] Autor:Zhao M; LaoI QY; Zhao DH; Ma J; Ru GQ; He XL; Wang Z; Wang J
[Ad] Endereço:Department of Pathology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
[Ti] Título:[Clinicopathologic and molecular genetic characterizations of biphenotypic sinonasal sarcoma].
[So] Source:Zhonghua Bing Li Xue Za Zhi;46(12):841-846, 2017 Dec 08.
[Is] ISSN:0529-5807
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the clinicopathologic characteristics, immunophenotypes, molecular genetics, and diagnostic and differential diagnostic features of biphenotypic sinonasal sarcoma (BSNS). Three cases of BSNS were retrieved, the histomorphology, immunophenotype and molecular genetics were analyzed with review of literature. There were 2 male and 1 female patient aged 45, 29 and 40 years, respectively.Computed tomography and magnetic resonance imaging examinations showed a large polypoid mass occupying the sinonasal cavity in all 3 patients. Microscopically, these tumors were un-circumscribed and composed of cellular spindle-shaped cells arranged in long and interlaced fascicles. A hemangiopericytoma-like growth pattern was frequently identified. The overlying hyperplastic respiratory epithelium invaginated down into the tumor forming a cystic (2 cases), glandular (1 case) structures and inverted in a papilloma-like (1 case)pattern, and foci of eosinophilic metaplasia were also noted in 2 of the three cases. The tumor nuclei were bland-appearing, mitoses were scarce and necrosis was absent. Immunohistochemically, the tumor cells showed co-expression of neural and myogenic markers in all the 3 cases, including that 3/3 showed diffuse and strong positivity of S-100 protein, 3/3 positivity of smooth muscle actin (1 diffuse and 2 focal), 1/2 diffuse positivity of calponin, 1/3 focal positivity of desmin, and 1/1 focal positivity of MyoD1.In addition, 1 detected for ß-catenin showed focal nuclear positivity. None of the 3 showed positivity to cytokeratin, CD34 or SOX10 in the tumor cells.Ki-67 showed an index <5%, 10% and <2%, respectively. Fluorescence in situ hybridization analysis showed rearrangements of PAX3 gene in all 3 cases. In case 3, reverse transcription polymerase chain reaction, followed by Sanger sequencing, demonstrated an in-frame fusion between PAX3 and FOXO1.Follow-up information (range 3-15 months)showed no evidence of local recurrence or distant metastasis in three cases. BSNS is a newly described entity which can be readily confused with a variety of benign and malignant spindle cell tumors encountered in the sinonasal cavity; immunohistochemistry co-expression of neural and myogenic markers and PAX3 gene rearrangement can help distinguish this tumor from its many mimickers.
[Mh] Termos MeSH primário: Neoplasias dos Seios Paranasais/genética
Neoplasias dos Seios Paranasais/patologia
Sarcoma/genética
Sarcoma/patologia
[Mh] Termos MeSH secundário: Adulto
Biomarcadores Tumorais/análise
Núcleo Celular
Desmina/análise
Diagnóstico Diferencial
Feminino
Rearranjo Gênico
Hemangiopericitoma/patologia
Seres Humanos
Imuno-Histoquímica
Imunofenotipagem
Hibridização in Situ Fluorescente
Queratinas/análise
Masculino
Meia-Idade
Recidiva Local de Neoplasia
Fator de Transcrição PAX3/genética
Neoplasias dos Seios Paranasais/química
Neoplasias dos Seios Paranasais/imunologia
Proteínas S100/análise
Fatores de Transcrição SOXE/análise
Sarcoma/química
Sarcoma/imunologia
beta Catenina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (CTNNB1 protein, human); 0 (Desmin); 0 (PAX3 Transcription Factor); 0 (PAX3 protein, human); 0 (S100 Proteins); 0 (SOXE Transcription Factors); 0 (beta Catenin); 68238-35-7 (Keratins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-5807.2017.12.006


  9 / 11167 MEDLINE  
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[PMID]:29304038
[Au] Autor:Madhankumar AB; Mrowczynski OD; Slagle-Webb B; Ravi V; Bourcier AJ; Payne R; Harbaugh KS; Rizk E; Connor JR
[Ad] Endereço:Department of Neurosurgery, Pennsylvania State University College of Medicine, Hershey, PA, United States of America.
[Ti] Título:Tumor targeted delivery of doxorubicin in malignant peripheral nerve sheath tumors.
[So] Source:PLoS One;13(1):e0181529, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peripheral nerve sheath tumors are benign tumors that have the potential to transform into malignant peripheral nerve sheath tumors (MPNSTs). Interleukin-13 receptor alpha 2 (IL13Rα2) is a cancer associated receptor expressed in glioblastoma and other invasive cancers. We analyzed IL13Rα2 expression in several MPNST cell lines including the STS26T cell line, as well as in several peripheral nerve sheath tumors to utilize the IL13Rα2 receptor as a target for therapy. In our studies, we demonstrated the selective expression of IL13Rα2 in several peripheral nerve sheath tumors by immunohistochemistry (IHC) and immunoblots. We established a sciatic nerve MPNST mouse model in NIH III nude mice using a luciferase transfected STS26T MPNST cell line. Similarly, analysis of the mouse sciatic nerves after tumor induction revealed significant expression of IL13Rα2 by IHC when compared to a normal sciatic nerve. IL13 conjugated liposomal doxorubicin was formulated and shown to bind and internalized in the MPNST cell culture model demonstrating cytotoxic effect. Our subsequent in vivo investigation in the STS26T MPNST sciatic nerve tumor model indicated that IL13 conjugated liposomal doxorubicin (IL13LIPDXR) was more effective in inhibiting tumor progression compared to unconjugated liposomal doxorubicin (LIPDXR). This further supports that IL13 receptor targeted nanoliposomes is a potential approach for treating MPNSTs.
[Mh] Termos MeSH primário: Antibióticos Antineoplásicos/administração & dosagem
Doxorrubicina/análogos & derivados
Neoplasias da Bainha Neural/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antibióticos Antineoplásicos/farmacocinética
Linhagem Celular Tumoral
Doxorrubicina/administração & dosagem
Doxorrubicina/farmacocinética
Sistemas de Liberação de Medicamentos
Seres Humanos
Imuno-Histoquímica
Interleucina-13/administração & dosagem
Subunidade alfa2 de Receptor de Interleucina-13/metabolismo
Antígeno Ki-67/metabolismo
Camundongos
Camundongos Nus
Neoplasias da Bainha Neural/imunologia
Neoplasias da Bainha Neural/metabolismo
Polietilenoglicóis/administração & dosagem
Polietilenoglicóis/farmacocinética
Proteínas S100/metabolismo
Neuropatia Ciática/tratamento farmacológico
Neuropatia Ciática/imunologia
Neuropatia Ciática/metabolismo
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (Interleukin-13); 0 (Interleukin-13 Receptor alpha2 Subunit); 0 (Ki-67 Antigen); 0 (Mki67 protein, mouse); 0 (S100 Proteins); 0 (liposomal doxorubicin); 30IQX730WE (Polyethylene Glycols); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181529


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[PMID]:29248577
[Au] Autor:Chen G; Sun W; Hua X; Zeng W; Yang L
[Ad] Endereço:Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China. Electronic address: gangchenwhu@sina.com.
[Ti] Título:Long non-coding RNA FOXD2-AS1 aggravates nasopharyngeal carcinoma carcinogenesis by modulating miR-363-5p/S100A1 pathway.
[So] Source:Gene;645:76-84, 2018 Mar 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Long non-coding RNAs (lncRNAs) have been wildly verified to modulate multiple tumorigenesis, especially nasopharyngeal carcinoma (NPC). In present study, we aim to investigate the role of lncRNA FOXD2-AS1 in the NPC carcinogenesis. It was indicated that FOXD2-AS1 was markedly increased in NPC tissues and cells in comparison to their corresponding controls. Moreover, the aberrant overexpression of FOXD2-AS1 indicated the poor prognosis of NPC patients. Silence of FOXD2-AS1 was able to repress NPC cell growth in vitro while overexpression of FOXD2-AS1 inversed this process. Moreover, in vivo tumor xenografts were established using CNE-1/SUNE-1 cells to investigate the function of FOXD2-AS1 in NSCLC tumorigenesis. Rescue assay was performed to further confirm that FOXD2-AS1 contributed to NPC progression by regulating miR-363-5p/S100A1 signal pathway. Taken together, our study discovered the oncogenic role of FOXD2-AS1 in clinical specimens and cellular experiments, showing the potential FOXD2-AS1/miR-363-5p/S100A1 pathway. This results and findings provide a novel insight for NPC tumorigenesis.
[Mh] Termos MeSH primário: Carcinoma/genética
MicroRNAs/genética
Neoplasias Nasofaríngeas/genética
RNA Longo não Codificante/genética
Proteínas S100/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Sobrevivência Celular
Progressão da Doença
Perfilação da Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Camundongos
Transplante de Neoplasias
Prognóstico
Transdução de Sinais
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN363 microRNA, human); 0 (MicroRNAs); 0 (RNA, Long Noncoding); 0 (S100 Proteins); 0 (S100A1 protein); 0 (long non-coding RNA FOXD2-AS1, human)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE



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