Base de dados : MEDLINE
Pesquisa : D12.776.157.170.125 [Categoria DeCS]
Referências encontradas : 388 [refinar]
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[PMID]:28704336
[Au] Autor:Decuypere JP; Ceulemans LJ; Wylin T; Martinet W; Monbaliu D; Pirenne J; Jochmans I
[Ad] Endereço:1 Laboratory of Abdominal Transplantation, Department of Microbiology and Immunology, KU Leuven, Belgium. 2 Department of Abdominal Transplant Surgery, University Hospitals Leuven, Leuven, Belgium. 3 Laboratory of Physiopharmacology, Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium.
[Ti] Título:Plasmatic Villin 1 Is a Novel In Vivo Marker of Proximal Tubular Cell Injury During Renal Ischemia-Reperfusion.
[So] Source:Transplantation;101(11):e330-e336, 2017 Nov.
[Is] ISSN:1534-6080
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Early detection of acute kidney injury (AKI), a common condition with a high mortality risk, can be facilitated by specific and reliable biomarkers. Villin 1, a protein typically found in the brush borders of proximal tubular cells, has been detected in urine of patients with AKI, but its possible release in plasma remains unexplored. METHODS: We measured the presence of villin 1 by immunohistochemistry on kidney sections and by Western blotting in plasma samples from rats subjected to renal ischemia-reperfusion injury, pigs subjected to renal transplantation and liver transplantation patients that developed AKI. Moreover, rats were treated with necrostatin-1, an inhibitor of programmed necrosis (necroptosis), which occurs in renal tubular cells during AKI. Villin 1 levels were compared with other renal injury markers (creatinine, aspartate transaminase, and heart-type fatty acid binding protein). RESULTS: During AKI, plasmatic villin 1 levels corresponded with the severity of kidney injury and dysfunction. Its detection in plasma was associated with a redistribution in the kidney tissue. Unlike the levels of other markers, plasmatic villin 1 decreased already after a short (3 hours) treatment with necrostatin-1 during renal ischemia-reperfusion injury. The presence of plasmatic villin 1 was confirmed in patients who experienced AKI after liver transplantation. CONCLUSIONS: Villin 1 is released in plasma during AKI and shows potential as an early marker for proximal tubular injury/necrosis and warrants further investigation.
[Mh] Termos MeSH primário: Lesão Renal Aguda/sangue
Túbulos Renais Proximais/metabolismo
Proteínas dos Microfilamentos/sangue
Traumatismo por Reperfusão/sangue
[Mh] Termos MeSH secundário: Lesão Renal Aguda/etiologia
Lesão Renal Aguda/patologia
Lesão Renal Aguda/prevenção & controle
Animais
Aspartato Aminotransferases/sangue
Biomarcadores/sangue
Creatinina/sangue
Modelos Animais de Doenças
Diagnóstico Precoce
Proteína 3 Ligante de Ácido Graxo
Proteínas de Ligação a Ácido Graxo/sangue
Feminino
Imidazóis/farmacologia
Indóis/farmacologia
Transplante de Rim/efeitos adversos
Túbulos Renais Proximais/efeitos dos fármacos
Túbulos Renais Proximais/patologia
Transplante de Fígado/efeitos adversos
Necrose
Valor Preditivo dos Testes
Ratos Sprague-Dawley
Traumatismo por Reperfusão/etiologia
Traumatismo por Reperfusão/patologia
Traumatismo por Reperfusão/prevenção & controle
Sus scrofa
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (FABP3 protein, rat); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 0 (Imidazoles); 0 (Indoles); 0 (Microfilament Proteins); 0 (necrostatin-1); 0 (villin protein, rat); 0 (villin1 protein, human); AYI8EX34EU (Creatinine); EC 2.6.1.1 (Aspartate Aminotransferases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1097/TP.0000000000001876


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[PMID]:28419114
[Au] Autor:Lagerstedt L; Egea-Guerrero JJ; Bustamante A; Montaner J; Rodríguez-Rodríguez A; El Rahal A; Turck N; Quintana M; García-Armengol R; Prica CM; Andereggen E; Rinaldi L; Sarrafzadeh A; Schaller K; Sanchez JC
[Ad] Endereço:Department of Human Protein Sciences, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
[Ti] Título:H-FABP: A new biomarker to differentiate between CT-positive and CT-negative patients with mild traumatic brain injury.
[So] Source:PLoS One;12(4):e0175572, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The majority of patients with mild traumatic brain injury (mTBI) will have normal Glasgow coma scale (GCS) of 15. Furthermore, only 5%-8% of them will be CT-positive for an mTBI. Having a useful biomarker would help clinicians evaluate a patient's risk of developing intracranial lesions. The S100B protein is currently the most studied and promising biomarker for this purpose. Heart fatty-acid binding protein (H-FABP) has been highlighted in brain injury models and investigated as a biomarker for stroke and severe TBI, for example. Here, we evaluate the performances of S100B and H-FABP for differentiating between CT-positive and CT-negative patients. A total of 261 patients with a GCS score of 15 and at least one clinical symptom of mTBI were recruited at three different European sites. Blood samples from 172 of them were collected ≤ 6 h after trauma. Patients underwent a CT scan and were dichotomised into CT-positive and CT-negative groups for statistical analyses. H-FABP and S100B levels were measured using commercial kits, and their capacities to detect all CT-positive scans were evaluated, with sensitivity set to 100%. For patients recruited ≤ 6 h after trauma, the CT-positive group demonstrated significantly higher levels of both H-FABP (p = 0.004) and S100B (p = 0.003) than the CT-negative group. At 100% sensitivity, specificity reached 6% (95% CI 2.8-10.7) for S100B and 29% (95% CI 21.4-37.1) for H-FABP. Similar results were obtained when including all the patients recruited, i.e. hospital arrival within 24 h of trauma onset. H-FABP out-performed S100B and thus seems to be an interesting protein for detecting all CT-positive mTBI patients with a GCS score of 15 and at least one clinical symptom.
[Mh] Termos MeSH primário: Biomarcadores/sangue
Concussão Encefálica/sangue
Concussão Encefálica/diagnóstico por imagem
Proteínas de Ligação a Ácido Graxo/sangue
Tomografia Computadorizada por Raios X/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Proteína 3 Ligante de Ácido Graxo
Feminino
Escala de Coma de Glasgow
Seres Humanos
Masculino
Meia-Idade
Admissão do Paciente
Subunidade beta da Proteína Ligante de Cálcio S100/sangue
Sensibilidade e Especificidade
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (FABP3 protein, human); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 0 (S100 Calcium Binding Protein beta Subunit); 0 (S100B protein, human)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175572


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[PMID]:28039809
[Au] Autor:Qin X; Xu A; Liu L; Sui Y; Li Y; Tan Y; Chen C; Xie Q
[Ad] Endereço:Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (MOE of China), Synergetic Innovation Center for Quantum Effects and Applications, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
[Ti] Título:Selective staining of CdS on ZnO biolabel for ultrasensitive sandwich-type amperometric immunoassay of human heart-type fatty-acid-binding protein and immunoglobulin G.
[So] Source:Biosens Bioelectron;91:321-327, 2017 May 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We report on an ultrasensitive metal-labeled amperometric immunoassay of proteins, which is based on the selective staining of nanocrystalline cadmium sulfide (CdS) on ZnO nanocrystals and in-situ microliter-droplet anodic stripping voltammetry (ASV) detection on the immunoelectrode. Briefly, antibody 1 (Ab ), bovine serum albumin (BSA), antigen and ZnO-multiwalled carbon nanotubes (MWCNTs) labeled antibody 2 (Ab -ZnO-MWCNTs) were successively anchored on a ß-cyclodextrin-graphene sheets (CD-GS) nanocomposite modified glassy carbon electrode (GCE), forming a sandwich-type immunoelectrode (Ab -ZnO-MWCNTs/antigen/BSA/Ab /CD-GS/GCE). CdS was selectively grown on the catalytic ZnO surfaces through chemical reaction of Cd(NO ) and thioacetamide (ZnO-label/CdS-staining), due to the presence of an activated cadmium hydroxide complex on ZnO surfaces that can decompose thioacetamide. A beforehand cathodic "potential control" in air and then injection of 7µL of 0.1M aqueous HNO on the immunoelectrode allow dissolution of the stained CdS and simultaneous cathodic preconcentration of atomic Cd onto the electrode surface, thus the following in-situ ASV detection can be used for immunoassay with enhanced sensitivity. Under optimized conditions, human immunoglobulin G (IgG) and human heart-type fatty-acid-binding protein (FABP) are analyzed by this method with ultrahigh sensitivity, excellent selectivity and small reagent-consumption, and the limits of detection (LODs, S/N=3) are 0.4fgmL for IgG and 0.3fgmL for FABP (equivalent to 73 FABP molecules in the 6µL sample employed).
[Mh] Termos MeSH primário: Anticorpos Imobilizados/química
Compostos de Cádmio/química
Técnicas Eletroquímicas/instrumentação
Proteínas de Ligação a Ácido Graxo/sangue
Imunoglobulina G/sangue
Nanopartículas/química
Sulfetos/química
Óxido de Zinco/química
[Mh] Termos MeSH secundário: Técnicas Biossensoriais/instrumentação
Eletrodos
Desenho de Equipamento
Proteína 3 Ligante de Ácido Graxo
Seres Humanos
Imunoensaio/instrumentação
Limite de Detecção
Nanopartículas/ultraestrutura
Nanotubos de Carbono/química
Nanotubos de Carbono/ultraestrutura
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Immobilized); 0 (Cadmium Compounds); 0 (FABP3 protein, human); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 0 (Immunoglobulin G); 0 (Nanotubes, Carbon); 0 (Sulfides); 057EZR4Z7Q (cadmium sulfide); SOI2LOH54Z (Zinc Oxide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170101
[St] Status:MEDLINE


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[PMID]:27864793
[Au] Autor:Baran A; Swiderska M; Bacharewicz-Szczerbicka J; Mysliwiec H; Flisiak I
[Ad] Endereço:Department of Dermatology and Venereology, Medical University of Bialystok, Zurawia 14 St, 15-540, Bialystok, Poland. aannabaran@wp.pl.
[Ti] Título:Serum Fatty Acid-Binding Protein 4 is Increased in Patients with Psoriasis.
[So] Source:Lipids;52(1):51-60, 2017 Jan.
[Is] ISSN:1558-9307
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Psoriasis is associated with metabolic syndrome and cardiovascular disease. Fatty acid-binding proteins (FABP) have been recognized as predictors of these systemic disorders. The aim of this study was to assess correlations between levels of serum heart and adipocyte fatty acid-binding proteins (FABP3, FABP4) and disease severity, indicators of inflammation or metabolic disturbances, and topical treatment in psoriatic patients. Thirty-seven patients with relapse of plaque-type psoriasis and 16 healthy volunteers were recruited. Blood samples were collected before and after 14 days of therapy. Serum FABP concentrations were examined by enzyme-linked immunosorbent assay for correlation with Psoriasis Area and Severity Index (PASI), body mass index (BMI), inflammatory or metabolic parameters, and treatment used. The median FABP4 serum levels were significantly increased (p = 0.038) in psoriatic patients, while FABP3 levels did not differ (p = 0.47) compared to the controls. No significant correlations were noted between the proteins and PASI, C-reactive protein (CRP), BMI, or levels of glucose or lipids. FABP3 significantly correlated with white blood count (p = 0.03) and aspartate aminotransferase (p = 0.04). After topical treatment, there was no significant change in serum FABP3 [11.5 (4.9-30.3) vs. 12.9 (3.5-30.3) ng/ml] (p = 0.96), whereas FABP4 was decreased [27,286 (20,344-32,257) vs. 23,034 (18,320-29,874) pg/ml] (p = 0.12), losing its basal significance. FABP4 may be a marker of psoriasis, and FABP3 may be associated with inflammation or liver disorders in psoriatic patients. FABP do not appear to be useful for determining disease severity or the effectiveness of antipsoriatic treatment.
[Mh] Termos MeSH primário: Antralina/administração & dosagem
Proteínas de Ligação a Ácido Graxo/sangue
Psoríase/tratamento farmacológico
Ácido Salicílico/administração & dosagem
[Mh] Termos MeSH secundário: Administração Tópica
Adulto
Idoso
Idoso de 80 Anos ou mais
Antralina/farmacologia
Proteína 3 Ligante de Ácido Graxo
Feminino
Seres Humanos
Masculino
Meia-Idade
Estudos Prospectivos
Psoríase/metabolismo
Ácido Salicílico/farmacologia
Índice de Gravidade de Doença
Resultado do Tratamento
Regulação para Cima/efeitos dos fármacos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FABP3 protein, human); 0 (FABP4 protein, human); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); O414PZ4LPZ (Salicylic Acid); U8CJK0JH5M (Anthralin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE
[do] DOI:10.1007/s11745-016-4211-4


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[PMID]:27859493
[Au] Autor:Tiburcio TC; Willebrords J; da Silva TC; Pereira IV; Nogueira MS; Crespo Yanguas S; Maes M; Silva ED; Dagli ML; de Castro IA; Oliveira CP; Vinken M; Cogliati B
[Ad] Endereço:Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
[Ti] Título:Connexin32 deficiency is associated with liver injury, inflammation and oxidative stress in experimental non-alcoholic steatohepatitis.
[So] Source:Clin Exp Pharmacol Physiol;44(2):197-206, 2017 Feb.
[Is] ISSN:1440-1681
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Non-alcoholic steatohepatitis is a highly prevalent liver pathology featured by hepatocellular fat deposition and inflammation. Connexin32, which is the major building block of hepatocellular gap junctions, has a protective role in hepatocarcinogenesis and is downregulated in chronic liver diseases. However, the role of connexin32 in non-alcoholic steatohepatitis remains unclear. Connexin32 mice and their wild-type littermates were fed a choline-deficient high-fat diet. The manifestation of non-alcoholic steatohepatitis was evaluated based on a battery of clinically relevant read-outs, including histopathological examination, diverse indicators of inflammation and liver damage, in-depth lipid analysis, assessment of oxidative stress, insulin and glucose tolerance, liver regeneration and lipid-related biomarkers. Overall, more pronounced liver damage, inflammation and oxidative stress were observed in connexin32 mice compared to wild-type animals. No differences were found in insulin and glucose tolerance measurements and liver regeneration. However, two lipid-related genes, srebf1 and fabp3, were upregulated in Cx32 mice in comparison with wild-type animals. These findings suggest that connexin32-based signalling is not directly involved in steatosis as such, but rather in the sequelae of this process, which underlie progression of non-alcoholic steatohepatitis.
[Mh] Termos MeSH primário: Conexinas/deficiência
Citocinas/metabolismo
Fígado
Hepatopatia Gordurosa não Alcoólica/metabolismo
Estresse Oxidativo
[Mh] Termos MeSH secundário: Animais
Conexinas/genética
Citocinas/sangue
Proteína 3 Ligante de Ácido Graxo
Proteínas de Ligação a Ácido Graxo/genética
Junções Comunicantes/metabolismo
Metabolismo dos Lipídeos/genética
Lipídeos/sangue
Fígado/imunologia
Fígado/metabolismo
Fígado/ultraestrutura
Regeneração Hepática
Masculino
Camundongos Endogâmicos C57BL
Camundongos Knockout
Hepatopatia Gordurosa não Alcoólica/imunologia
Hepatopatia Gordurosa não Alcoólica/patologia
Estresse Oxidativo/genética
Proteína de Ligação a Elemento Regulador de Esterol 1/genética
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Connexins); 0 (Cytokines); 0 (Fabp3 protein, mouse); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 0 (Lipids); 0 (Srebf1 protein, mouse); 0 (Sterol Regulatory Element Binding Protein 1); 0 (connexin 32)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1111/1440-1681.12701


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[PMID]:27780314
[Au] Autor:Zhang Y; Huang R; Zhou W; Zhao Q; Lü Z
[Ad] Endereço:Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
[Ti] Título:miR-192-5p mediates hypoxia/reoxygenation-induced apoptosis in H9c2 cardiomyocytes via targeting of FABP3.
[So] Source:J Biochem Mol Toxicol;31(4), 2017 Apr.
[Is] ISSN:1099-0461
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Myocardial ischemia/reperfusion (I/R) injury is a leading cause of morbidity and mortality. In this study, we investigated the role of miR-192-5p in hypoxia/reoxygenation (H/R)-induced cardiomyocyte apoptosis. H9c2 cardiomyocytes were subjected to H/R and tested for miR-192-5p expression. Overexpression and knockdown experiments were performed to determine the effects of manipulating miR-192-5p on apoptotic responses. H/R-treated H9c2 cells exhibited a 2.2-fold increase in miR-192-5p levels. Overexpression of miR-192-5p significantly augmented apoptosis in H9c2 cells after H/R, which was accompanied by a significant increase in the ratio of Bax/Bcl-2. In contrast, delivery of anti-miR-192-5p inhibitors significantly reduced apoptosis induced by H/R. FABP3 was identified to be a functional target of miR-192-5p. Restoration of FABP3 prevented apoptosis in miR-192-5p-transfected H9c2 cells, whereas downregulation of FABP3 enhanced apoptosis in H/R-exposed H9c2 cells. In conclusion, miR-192-5p mediates H/R-induced apoptosis in cardiomyocytes by targeting FABP3 and represents a potential target for prevention of myocardial I/R injury.
[Mh] Termos MeSH primário: Apoptose
Hipóxia Celular
Proteínas de Ligação a Ácido Graxo
MicroRNAs
Miócitos Cardíacos/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Proteína 3 Ligante de Ácido Graxo
Traumatismo por Reperfusão Miocárdica/metabolismo
Traumatismo por Reperfusão Miocárdica/fisiopatologia
Miócitos Cardíacos/fisiologia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FABP3 protein, rat); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 0 (MIRN192 microRNA, human); 0 (MicroRNAs)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1002/jbt.21873


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[PMID]:27545084
[Au] Autor:Kakoti A; Goswami P
[Ad] Endereço:Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
[Ti] Título:Multifaceted analyses of the interactions between human heart type fatty acid binding protein and its specific aptamers.
[So] Source:Biochim Biophys Acta;1861(1 Pt A):3289-3299, 2017 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Aptamer-protein interaction studies have been mainly confined to dissociation constant (K ) determination. A combinatorial approach involving limited proteolysis mass spectroscopy, molecular docking and CD studies is reported here to elucidate the specific interactions involved. METHODS: To generate aptamers specific for human FABP3, SELEX was performed incorporating counter SELEX cycles against control FABPs and GST tag, followed by their characterization by EMSA, CD and SVD analysis. Based on computationally obtained aptamer-protein complex models, the interacting aptamer, and protein residues were predicted and supported by limited proteolysis experiments. RESULTS: Two aptamers N13 and N53 specific for human fatty acid binding protein (FABP3) were isolated with corresponding K of 0.0743±0.0142µM and 0.3337±0.1485µM for FABP3 interactions. Both aptamers possess stable B-DNA structures at salt concentration of 100mM and pH range (6-9). The N13 aptamer led interaction involved 3 salt bridges and 2 hydrogen bonds, whereas N53 had 2 salt bridges with 8 hydrogen and 7 hydrophobic interactions. CONCLUSIONS: The aptamers generated are the first to be reported against human FABP3. The higher interaction footprint of N53 incited synergistic conformational changes in both N53 and FABP3 during interaction, leading to a decline in binding affinity in comparison to N13 which corroborated to the calculated K values. GENERAL SIGNIFICANCE: This combinatorial method may be used to retrieve the possible specific binding modes and interaction patterns involved in large aptamer-protein complexes. Thus the method can be exploited to identify the optimum aptamer length for in-depth structure-function studies and its tailored applications.
[Mh] Termos MeSH primário: Aptâmeros de Nucleotídeos/metabolismo
Proteínas de Ligação a Ácido Graxo/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/metabolismo
Aptâmeros de Nucleotídeos/química
Aptâmeros de Nucleotídeos/genética
Sequência de Bases
Dicroísmo Circular
Biologia Computacional
Ensaio de Desvio de Mobilidade Eletroforética
Proteína 3 Ligante de Ácido Graxo
Proteínas de Ligação a Ácido Graxo/química
Seres Humanos
Simulação de Acoplamento Molecular
Desnaturação de Ácido Nucleico
Nucleotídeos/metabolismo
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/metabolismo
Ligação Proteica
Proteólise
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids); 0 (Aptamers, Nucleotide); 0 (FABP3 protein, human); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 0 (Nucleotides); 0 (Peptide Fragments)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160823
[St] Status:MEDLINE


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[PMID]:27150386
[Au] Autor:Abass MA; Arafa MH; El-Shal AS; Atteia HH
[Ad] Endereço:1 Department of Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Skarkia, Egypt.
[Ti] Título:Asymmetric dimethylarginine and heart-type fatty acid-binding protein 3 are risk markers of cardiotoxicity in carbon monoxide poisoning cases in Zagazig university hospitals.
[So] Source:Hum Exp Toxicol;36(3):247-255, 2017 Mar.
[Is] ISSN:1477-0903
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Carbon monoxide (CO) poisoning is a leading cause of toxicity-related mortality and morbidity worldwide. Recent studies focused on CO-induced cardiovascular toxicity. Oxidative stress plays an important role in the pathophysiology of CO toxicity. The aim of this study was to elucidate the relationship between cardiac damage biomarkers and oxidative stress biomarkers in patients with CO-induced cardiotoxicity. This study was carried out on 36 CO-poisoned patients admitted to Zagazig University Hospitals. Forty healthy individuals (age- and sex-matched) were selected as a control group. Clinical examination and electrocardiography (ECG) were performed for CO-poisoned patients. These patients have been investigated for carboxyhaemoglobin percent (COHB%) and cardiac damage biomarkers; cardiac troponin I (cTn-I), heart-type fatty acid-binding protein 3 (H-FABP3). Oxidative stress biomarkers comprising malondialdehyde (MDA), asymmetric dimethylarginine (ADMA), and total antioxidant capacity (TAC) have been also assessed. All biomarkers have been assessed on admission (0 h) and 6 h after treatment of CO-poisoned patients with high-flow oxygen and compared with those of the control groups. ECG findings were abnormal in 31 patients (86.11%), where sinus tachycardia was the commonest finding (58.33%). There was a statistically significant increase of COHB%, MDA, ADMA, and H-FABP3 levels, and a significant decrease of TAC level in CO-poisoned patients compared to controls with no significant changes in cTn-I. Six hours following treatment, all measured parameters were significantly improved except for cTn-I, which was significantly increased when compared with admission status (0 h). Furthermore, H-FABP3 showed a significant positive correlation with COHB%, MDA, ADMA, and a negative correlation with TAC, while cTn-I was significantly correlated with COHB% only. ADMA and MDA seem to be the strongest determinants for the prediction of H-FABP3 changes and hence cardiovascular toxicity. Thus, cardiac damage in patients with CO poisoning could be partially mediated by CO-induced oxidative stress, where H-FABP3 level was directly and strongly associated with MDA and ADMA levels.
[Mh] Termos MeSH primário: Arginina/análogos & derivados
Biomarcadores/metabolismo
Intoxicação por Monóxido de Carbono/metabolismo
Proteínas de Ligação a Ácido Graxo/metabolismo
Coração/efeitos dos fármacos
Coração/fisiopatologia
[Mh] Termos MeSH secundário: Adulto
Arginina/metabolismo
Intoxicação por Monóxido de Carbono/fisiopatologia
Carboxihemoglobina/metabolismo
Estudos de Casos e Controles
Egito
Eletrocardiografia
Proteína 3 Ligante de Ácido Graxo
Feminino
Seres Humanos
Masculino
Fatores de Risco
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (FABP3 protein, human); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 63CV1GEK3Y (N,N-dimethylarginine); 9061-29-4 (Carboxyhemoglobin); 94ZLA3W45F (Arginine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE
[do] DOI:10.1177/0960327116646621


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[PMID]:27966485
[Au] Autor:Garcia-Rua V; Feijoo-Bandin S; Garcia-Vence M; Aragon-Herrera A; Bravo SB; Rodriguez-Penas D; Mosquera-Leal A; Lear PV; Parrington J; Alonso J; Rosello-Lleti E; Portoles M; Rivera M; Gonzalez-Juanatey JR; Lago F
[Ad] Endereço:Cellular and Molecular Cardiology Research Unit and Department of Cardiology, Institute of Biomedical Research and University Clinical Hospital, 15706, Santiago de Compostela, Spain.
[Ti] Título:Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level.
[So] Source:J Biosci;41(4):643-658, 2016 Dec.
[Is] ISSN:0973-7138
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Two-pore channels (TPCs or TPCNs) are novel voltage-gated ion channels that have been postulated to act as Ca2+ and/or Na+ channels expressed exclusively in acidic organelles such as endosomes and lysosomes. TPCNs participate in the regulation of diverse biological processes and recently have been proposed to be involved in the pathophysiology of metabolic disorders such as obesity, fatty liver disease and type 2 diabetes mellitus. Due to the importance of these pathologies in the development of cardiovascular diseases, we aimed to study the possible role of two-pore channel 1 (TPCN1) in the regulation of cardiac metabolism. To explore the cardiac function of TPCN1, we developed proteomic approaches as 2-DE-MALDI-MS and LC-MALDI-MS in the cardiac left ventricle of TPCN1 KO and WT mice, and found alterations in several proteins implicated in glucose and fatty acid metabolism in TPCN1 KO vs. WT mice. The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process. Finally, in vitro experiments performed in neonatal rat cardiomyocytes, in which TPCN1 was silenced using siRNAs, confirmed that the downregulation of TPCN1 gene expression increased 2-deoxy-D-[3H]-glucose uptake and GLUT4 mobilization into cell peripherals in cardiac cells. Our results are the first to suggest a potential role for TPCNs in cardiac metabolism regulation.
[Mh] Termos MeSH primário: Canais de Cálcio/genética
Proteínas de Ligação a Ácido Graxo/biossíntese
Transportador de Glucose Tipo 4/biossíntese
Fosfoglicerato Quinase/biossíntese
Fosfopiruvato Hidratase/biossíntese
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Canais de Cálcio/biossíntese
Doenças Cardiovasculares/etiologia
Doenças Cardiovasculares/genética
Doenças Cardiovasculares/patologia
Diabetes Mellitus Tipo 2/complicações
Diabetes Mellitus Tipo 2/genética
Diabetes Mellitus Tipo 2/patologia
Proteína 3 Ligante de Ácido Graxo
Proteínas de Ligação a Ácido Graxo/genética
Regulação da Expressão Gênica
Transportador de Glucose Tipo 4/genética
Seres Humanos
Metabolismo dos Lipídeos/genética
Camundongos
Camundongos Knockout
Miocárdio/metabolismo
Miocárdio/patologia
Miócitos Cardíacos/metabolismo
Miócitos Cardíacos/patologia
Fosfoglicerato Quinase/genética
Fosfopiruvato Hidratase/genética
Proteômica
Ratos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Channels); 0 (Fabp3 protein, mouse); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 0 (Glucose Transporter Type 4); 0 (Slc2a4 protein, mouse); 0 (TPCN1 protein, mouse); EC 2.7.2.3 (Pgk1 protein, mouse); EC 2.7.2.3 (Phosphoglycerate Kinase); EC 4.2.1.11 (Phosphopyruvate Hydratase); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE


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[PMID]:27916210
[Au] Autor:Qian HY; Huang J; Yang YJ; Yang YM; Li ZZ; Zhang JM
[Ad] Endereço:Center for Coronary Heart Disease, Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
[Ti] Título:Heart-type Fatty Acid Binding Protein in the Assessment of Acute Pulmonary Embolism.
[So] Source:Am J Med Sci;352(6):557-562, 2016 Dec.
[Is] ISSN:1538-2990
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To explore the predictive value of heart-type fatty acid binding protein (H-FABP) in the stratification and prognosis of patients with acute pulmonary embolism (APE). METHODS: According to risk stratification, 69 patients with APE admitted into the emergency department within 24 hours after onset were divided into the following 3 groups: high-risk group, moderate-risk group and low-risk group. H-FABP- and cardiac troponin I (cTNI)-positive rates of all groups were analyzed and compared, and the correlation between major adverse events (death, endotracheal intubation and cardiopulmonary resuscitation) and the cardiac markers (heart rate, arterial partial pressure of oxygen, right ventricular dimension, pulmonary arterial pressure, etc.) during the in-hospital period were statistically analyzed. Then the prognosis (death, embolic pulmonary hypertension, right heart failure and recurrence of APE) at 6 months after onset of APE was followed-up on and compared between groups. RESULTS: The admission time of high-risk group patients was earlier than non-high-risk group (7.1 ± 2.9 versus 13.5 ± 6.7 versus 15.2 ± 10.7 hours, P = 0.001), had larger right ventricular dimension (33.1 ± 10.4 versus 26.7 ± 7.3 versus 20.5 ± 8.9mm, P = 0.002) and higher pulmonary arterial pressure (45.8 ± 14.6 versus 29.4 ± 13.9 versus 23.1 ± 12.6mmHg, P = 0.001). The major adverse events during in-hospital period, including death, endotracheal intubation and cardiopulmonary resuscitation, were more prevalent in the high-risk group than those in the other 2 risk groups. Further analysis indicated that the positive rate of H-FABP was remarkably higher than cTNI (52/69, 75.4% versus 28/69, 40.6%, P = 0.003). The H-FABP (r = 0.881, P = 0.020) was significantly correlated to the major adverse events; however, this was not so regarding cTNI (r = 0.115, P = 0.059). At 6 months after onset of APE, the follow-up data indicated that cTNI and H-FABP were both significantly correlated with the major adverse events. CONCLUSIONS: The positive rate of H-FABP was higher than cTNI during the 24 hours after the onset of APE. The H-FABP was significantly correlated to the major adverse events during hospitalization and to the primary prognosis at 6 months after onset of APE.
[Mh] Termos MeSH primário: Proteínas de Ligação a Ácido Graxo/sangue
Embolia Pulmonar/sangue
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores/sangue
China/epidemiologia
Proteína 3 Ligante de Ácido Graxo
Feminino
Seres Humanos
Masculino
Meia-Idade
Prognóstico
Embolia Pulmonar/diagnóstico
Embolia Pulmonar/mortalidade
Medição de Risco
Troponina I/sangue
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (FABP3 protein, human); 0 (Fatty Acid Binding Protein 3); 0 (Fatty Acid-Binding Proteins); 0 (Troponin I)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE



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