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[PMID]:28741159
[Au] Autor:Schmitz EJ; Herwig-Carl MC; Holz FG; Loeffler KU
[Ad] Endereço:Department of Ophthalmology, University Eye Hospital Bonn, University of Bonn, Ernst-Abbe Str. 2, 53127, Bonn, Germany. eva.schmitz@ukb.uni-bonn.de.
[Ti] Título:Sebaceous gland carcinoma of the ocular adnexa - variability in clinical and histological appearance with analysis of immunohistochemical staining patterns.
[So] Source:Graefes Arch Clin Exp Ophthalmol;255(11):2277-2285, 2017 Nov.
[Is] ISSN:1435-702X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The purpose of the study was to evaluate the characteristics of sebaceous gland carcinoma (SGC) of the ocular adnexae, which is due to a high variability in clinical, histological and immunohistochemical characteristics often challenging to diagnose. METHODS: Records of six patients with SGC were reviewed, who underwent surgical excision and who were histologically diagnosed with SGC. For comparison, there were specimens from four patients with basal cell carcinoma (BCC) and four patients with squamous cell carcinoma (SCC). Histological and immunohistochemical analysis included stains for HE, cytokeratins (CKpan, Cam5.2), epithelial membrane antigen (EMA), androgen receptor (AR441), perforin and adipophilin. RESULTS: SGC's were located in the upper (n = 2) or lower (n = 4) eyelid and were associated with various presenting clinical signs including chalazion-like lesions with pyogenic granuloma (n = 1), papillomatous conjunctival tumors (n = 3), a hyperkeratotic exophytic neoplasm (n = 1) and an ulcerating crusted lesion resembling chronic blepharitis (n = 1). The treatment was tumor resection, followed (if necessary) by adjuvant therapy with topical Mitomycin C (n = 2). Histologic characteristics included basophilic pleomorphic cells with vacuolated cytoplasm, prominent nucleoli, mitotic figures and in some cases pagetoid spread (n = 2). CKpan, EMA and Cam5.2 showed strong positive immunoreactivity in all specimens (SGC, BCC, SCC). Perforin immunostaining showed a varying, but overall weak, non-specific cytoplasmatic staining reaction in all lesions. AR441 positivity was noted with variable intensity in almost all lesions and in particular in pagetoid spread in contrast to non-tumor cells. Adipophilin showed an annular staining of lipid granules in immature sebaceous cells in SGC in contrast to a more granular staining pattern in BCC and SCC. CONCLUSION: SGCs display a variety of clinical signs and may mimic many other lesions. Tumor resection, followed by histological and immunohistochemical analysis, leads to the diagnosis and initiation of the proper treatment regimen. Herein, immunohistochemistry showed an unequivocal profile in SGC and did not allow for an exact differentiation from BCC and SCC by immunohistochemical means only. An extended evaluation of HE stains remains essential. However, immunohistochemistry can make relevant contributions to the diagnosis of SGC, especially in cases of inconclusive histology, by positive staining for adipophilin in immature sebaceous cells or by AR441 labeling in cases of pagetoid spread.
[Mh] Termos MeSH primário: Adenocarcinoma Sebáceo/patologia
Biomarcadores Tumorais/metabolismo
Neoplasias Palpebrais/patologia
Estadiamento de Neoplasias
Neoplasias das Glândulas Sebáceas/patologia
Glândulas Sebáceas/patologia
[Mh] Termos MeSH secundário: Adenocarcinoma Sebáceo/metabolismo
Adulto
Idoso
Idoso de 80 Anos ou mais
Neoplasias Palpebrais/metabolismo
Feminino
Seguimentos
Seres Humanos
Imuno-Histoquímica
Masculino
Meia-Idade
Perilipina-2/metabolismo
Estudos Retrospectivos
Neoplasias das Glândulas Sebáceas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Perilipin-2)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1007/s00417-017-3738-2


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[PMID]:28966044
[Au] Autor:Yan Y; Wang H; Hu M; Jiang L; Wang Y; Liu P; Liang X; Liu J; Li C; Lindström-Battle A; Lam SM; Shui G; Deng WM; Jiao R
[Ad] Endereço:Sino-French Hoffmann Institute, School of Basic Sciences, Guangzhou Medical University, Dongfengxi Road 195, Guangzhou 510182, China; State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, the Chinese Academy of Sciences, Datun Road 15, Beijing 100101, China.
[Ti] Título:HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.
[So] Source:Dev Cell;43(1):99-111.e5, 2017 Oct 09.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Age-dependent ectopic fat accumulation (EFA) in animals contributes to the progression of tissue aging and diseases such as obesity, diabetes, and cancer. However, the primary causes of age-dependent EFA remain largely elusive. Here, we characterize the occurrence of age-dependent EFA in Drosophila and identify HDAC6, a cytosolic histone deacetylase, as a suppressor of EFA. Loss of HDAC6 leads to significant age-dependent EFA, lipid composition imbalance, and reduced animal longevity on a high-fat diet. The EFA and longevity phenotypes are ameliorated by a reduction of the lipid-droplet-resident protein PLIN2. We show that HDAC6 is associated physically with the chaperone protein dHsc4/Hsc70 to maintain the proteostasis of PLIN2. These findings indicate that proteostasis collapse serves as an intrinsic cue to cause age-dependent EFA. Our study suggests that manipulation of proteostasis could be an alternative approach to the treatment of age-related metabolic diseases such as obesity and diabetes.
[Mh] Termos MeSH primário: Proteínas de Drosophila/metabolismo
Drosophila melanogaster/metabolismo
Gorduras/metabolismo
Histona Desacetilases/metabolismo
Gotículas Lipídicas/metabolismo
Perilipina-2/metabolismo
[Mh] Termos MeSH secundário: Envelhecimento
Animais
Autofagia/fisiologia
Citosol/metabolismo
Dieta Hiperlipídica
Drosophila melanogaster/genética
Desacetilase 6 de Histona
Longevidade/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (Fats); 0 (Perilipin-2); EC 3.5.1.98 (HDAC6 protein, Drosophila); EC 3.5.1.98 (Histone Deacetylase 6); EC 3.5.1.98 (Histone Deacetylases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


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[PMID]:28336294
[Au] Autor:Akoumi A; Haffar T; Mousterji M; Kiss RS; Bousette N
[Ad] Endereço:Universite de Montréal, Department of Biomedical Science, Canada; Montreal Heart Institute, Canada.
[Ti] Título:Palmitate mediated diacylglycerol accumulation causes endoplasmic reticulum stress, Plin2 degradation, and cell death in H9C2 cardiomyoblasts.
[So] Source:Exp Cell Res;354(2):85-94, 2017 May 15.
[Is] ISSN:1090-2422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have previously shown that palmitate causes ER stress in primary cardiomyocytes and this was associated with a diffuse lipid staining histology. In contrast, oleate, which was non-toxic, led to the formation of abundant, clearly delineated lipid droplets. The aberrant lipid histology in palmitate treated cells led us to hypothesize that perhaps there was an impairment in lipid droplet formation, which could lead to accumulation of lipids in the ER and consequent ER stress. To test this hypothesis we treated H9C2s (a cardiomyoblast cell line) with either 300µM oleate or palmitate for 8h. We found that palmitate resulted in significantly less lipid droplet abundance despite elevated intracellular lipid accumulation. Next we showed that palmitate was packaged primarily as diacylglycerol (DAG), in contrast oleate formed primarily triacylglycerol (TAG). Furthermore, the palmitate induced DAG accumulated mostly in the ER, while oleate treatment resulted in accumulation of TAG primarily in lipid droplets. The palmitate-induced accumulation of lipid in the ER was associated with a strong ER stress response. Interestingly, we found that ER stress induced by either palmitate, tunicamycin, or thapsigargin led to the degradation of Plin2, an important lipid droplet binding protein. In contrast palmitate had little effect on either Plin3 or Plin5. Furthermore, we found that acute MG132 administration significantly attenuated palmitate mediated ER stress and cell death. This protection was associated with a moderate attenuation of Plin2 degradation.
[Mh] Termos MeSH primário: Diglicerídeos/metabolismo
Estresse do Retículo Endoplasmático/efeitos dos fármacos
Miócitos Cardíacos/metabolismo
Miócitos Cardíacos/patologia
Ácido Palmítico/farmacologia
Perilipina-2/metabolismo
[Mh] Termos MeSH secundário: Animais
Morte Celular/efeitos dos fármacos
Linhagem Celular
Retículo Endoplasmático/efeitos dos fármacos
Retículo Endoplasmático/metabolismo
Ácidos Graxos/metabolismo
Leupeptinas/farmacologia
Gotículas Lipídicas/efeitos dos fármacos
Gotículas Lipídicas/metabolismo
Camundongos
Miócitos Cardíacos/efeitos dos fármacos
Ácido Oleico/farmacologia
Oxirredução/efeitos dos fármacos
Inibidores de Proteassoma/farmacologia
Proteólise/efeitos dos fármacos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1,2-diacylglycerol); 0 (Diglycerides); 0 (Fatty Acids); 0 (Leupeptins); 0 (Perilipin-2); 0 (Plin2 protein, rat); 0 (Proteasome Inhibitors); 2UMI9U37CP (Oleic Acid); 2V16EO95H1 (Palmitic Acid); RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


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[PMID]:28128380
[Au] Autor:Dossi CG; Cadagan C; San Martín M; Espinosa A; González-Mañán D; Silva D; Mancilla RA; Tapia GS
[Ad] Endereço:Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile. gtapia@med.uchile.cl.
[Ti] Título:Effects of rosa mosqueta oil supplementation in lipogenic markers associated with prevention of liver steatosis.
[So] Source:Food Funct;8(2):832-841, 2017 Feb 22.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Rosa mosqueta (RM) oil is rich in α-linolenic acid (ALA) - a precursor of eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), and it has a high antioxidant activity due to its abundant content of tocopherols. Additionally, it has been observed that RM oil administration prevents hepatic steatosis. Thus, the aim of this study was to demonstrate the antilipogenic mechanism related to RM oil administration in a high-fat diet (HFD) fed mice model by evaluating markers associated with the regulation of lipid droplet metabolism (PLIN2, PLIN5 and PPAR-γ), and proteins associated with lipogenesis (FAS and SREBP-1c). C57BL/6J mice were fed either a control diet or a HFD, with and without RM oil supplementation for 12 weeks. The results showed that RM oil supplementation decreases hepatic PLIN2 and PPAR-γ mRNA expression and SREBP-1c, FAS and PLIN2 protein levels, whereas we did not find changes in the level of PLIN5 among the groups. These results suggest that modulation of lipogenic markers could be one of the mechanisms, through which RM oil supplementation prevents the hepatic steatosis induced by HFD consumption in a mice model.
[Mh] Termos MeSH primário: Fígado Gorduroso/prevenção & controle
Óleos Vegetais/administração & dosagem
Rosa/química
[Mh] Termos MeSH secundário: Animais
Dieta Hiperlipídica/efeitos adversos
Suplementos Nutricionais/análise
Fígado Gorduroso/genética
Fígado Gorduroso/metabolismo
Seres Humanos
Lipogênese
Fígado/efeitos dos fármacos
Fígado/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
PPAR gama/genética
PPAR gama/metabolismo
Perilipina-2/genética
Perilipina-2/metabolismo
Perilipina-5/genética
Perilipina-5/metabolismo
Proteína de Ligação a Elemento Regulador de Esterol 1/genética
Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (PPAR gamma); 0 (Perilipin-2); 0 (Perilipin-5); 0 (Plant Oils); 0 (Plin2 protein, mouse); 0 (Plin5 protein, mouse); 0 (Sterol Regulatory Element Binding Protein 1)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1039/c6fo01762b


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[PMID]:28109910
[Au] Autor:Lin LC; Gao AC; Lai CH; Hsieh JT; Lin H
[Ad] Endereço:Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan; Department of Urology, University of Texas Southwestern Medical Center Dallas, TX, USA.
[Ti] Título:Induction of neuroendocrine differentiation in castration resistant prostate cancer cells by adipocyte differentiation-related protein (ADRP) delivered by exosomes.
[So] Source:Cancer Lett;391:74-82, 2017 04 10.
[Is] ISSN:1872-7980
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Although overall mortality rate of prostate cancer (PCa) declines in recent years, castration-resistant prostate cancer (CRPC) remains incurable. Clinical evidence indicates that CRPC recurred from hormonal therapy exhibits neuroendocrine differentiated (NED) phenotypes, which could contribute to therapeutic resistance and poor survival. Understanding the onset of NED could lead us to develop new therapeutic strategies for CRPC. Although PCa is known as a lipid-enriched tumor, its role in CRPC development is not fully understood. In this study, we demonstrated that IL-6 or androgen deprivation therapy (ADT)-induced lipid accumulation is associated with NED phenotypes. IL-6 or ADT can induce NED in PCa cells via peroxisome proliferator-activated receptor γ (PPARγ, a major lipogenic transcription factor) and adipocyte differentiation-related protein (ADRP, a major component of adiposome). In addition, ADRP protein can be detected in exosomes released from these cells and these exosomes are capable of inducing NED of PCa cells in a paracrine fashion. Understanding the role of PPARγ/ADRP in NED could provide new target(s) for CRPC therapy.
[Mh] Termos MeSH primário: Adipócitos/metabolismo
Exossomos/metabolismo
Perilipina-2/genética
Neoplasias de Próstata Resistentes à Castração/genética
[Mh] Termos MeSH secundário: Diferenciação Celular
Seres Humanos
Masculino
Perilipina-2/metabolismo
Neoplasias de Próstata Resistentes à Castração/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Perilipin-2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171029
[Lr] Data última revisão:
171029
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE


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[PMID]:27939924
[Au] Autor:Chen X; Firdaus SJ; Howard AD; Soulages JL; Arrese EL
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
[Ti] Título:Clues on the function of Manduca sexta perilipin 2 inferred from developmental and nutrition-dependent changes in its expression.
[So] Source:Insect Biochem Mol Biol;81:19-31, 2017 Feb.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cellular triglycerides (TG) are stored in cytosolic lipid droplets (LDs). Perilipins (PLIN) are a group of LD-proteins that play important roles in the assembly and transport of LDs and in TG metabolism. Two members of the PLIN family are found in insects (PLIN1 & 2 or Lsd1 & 2). We have cloned and expressed Manduca sexta PLIN2 (MsPLIN2), and studied developmental and nutritional changes in the expression of PLIN2. Nutritional changes induced fast alterations in PLIN2 mRNA and protein levels in fat body and midgut of the feeding larvae. The relationship observed between PLIN2 expression and TG synthesis in both larval fat body and midgut suggests that PLIN2 is needed when tissues are accumulating TG. However, when the fat body was storing TG at maximal capacity, MsPLIN2 levels declined. This unexpected finding suggests the occurrence of alternative mechanism/s to shield TG from the action of lipases in M. sexta LDs. In addition, it implies that the cellular level of lipid storage could be modulating MsPLIN2 expression and/or degradation. The study also confirmed that MsPLIN2 was most abundant in the adult fat body, which is characterized by a high rate of TG hydrolysis and lipid mobilization. Whether MsPLIN2 is directly involved in lipolysis and/or the secretion of lipids in the fat body of adult of M. sexta is unknown at this time. Nonetheless, the coexistence of high PLIN2 and lipolysis levels suggests a complex role for MsPLIN2. Altogether, we found that MsPLIN2 is needed when the synthesis of glycerides, DG and TG, is active even if the insect is accumulating or consuming TG.
[Mh] Termos MeSH primário: Corpo Adiposo/metabolismo
Metabolismo dos Lipídeos
Manduca/metabolismo
Perilipina-2/metabolismo
[Mh] Termos MeSH secundário: Animais
Trato Gastrointestinal/metabolismo
Manduca/crescimento & desenvolvimento
Análise de Sequência de DNA
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Perilipin-2); 0 (Triglycerides)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27886404
[Au] Autor:Fujimoto M; Matsuzaki I; Yamamoto Y; Yoshizawa A; Warigaya K; Iwahashi Y; Kojima F; Furukawa F; Murata SI
[Ad] Endereço:Department of Diagnostic Pathology, Wakayama Medical University, Wakayama, Japan.
[Ti] Título:Adipophilin expression in cutaneous malignant melanoma.
[So] Source:J Cutan Pathol;44(3):228-236, 2017 Mar.
[Is] ISSN:1600-0560
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The lipogenic pathway is upregulated in cancer cells, including melanomas. However, the pathological significance of cellular lipids in melanocytic lesions has yet to be determined. In this study, we evaluated intracytoplasmic lipid droplets in melanocytic nevi (MNs) and malignant melanomas via immunohistochemical analysis of adipophilin (ADP), which coats lipid droplets. METHODS: One hundred primary cutaneous melanocytic lesions [33 MNs, 17 melanomas in situ (MIS), and 50 invasive melanomas (IMs)] were immunostained for ADP. The intensity score (IS) and proportion score (PS) of ADP staining in each case was recorded semiquantitatively on a scale of 0 to 3+. RESULTS: High ADP expression (IS2/3+ and PS2/3+) was observed in 27 primary cutaneous melanocytic lesions that consisted of 23 IMs, three MISs, and one MN. Consequently, high ADP expression was associated with malignancy (38.8% vs. 3.0%; p < 0.0001). Among the IMs, high ADP expression was more prevalent in pT3/4 than pT1/2 (63.3% vs. 23.8%; p = 0.01) and Stage 3/4 than Stage 1/2 (76.9% vs. 36.8%; p = 0.02). CONCLUSIONS: The majority of the melanocytic lesions with high ADP expression were malignant melanomas in our cohort. Therefore, ADP expression may serve as a sensitive diagnostic marker for malignant melanoma.
[Mh] Termos MeSH primário: Melanoma/diagnóstico
Perilipina-2/biossíntese
Neoplasias Cutâneas/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Idoso
Biomarcadores Tumorais
Feminino
Seres Humanos
Imuno-Histoquímica
Masculino
Meia-Idade
Nevo Pigmentado/diagnóstico
Perilipina-2/análise
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (PLIN2 protein, human); 0 (Perilipin-2)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE
[do] DOI:10.1111/cup.12868


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[PMID]:27646609
[Au] Autor:Westhoff CC; Mrozinski J; Riedel I; Heid HW; Moll R
[Ad] Endereço:Institute of Pathology, Philipps University of Marburg and University Hospital Giessen and Marburg, Baldingerstrasse, 35043, Marburg, Germany. westhoff@med.uni-marburg.de.
[Ti] Título:Perilipin 1 is a highly specific marker for adipocytic differentiation in sarcomas with intermediate sensitivity.
[So] Source:J Cancer Res Clin Oncol;143(2):225-232, 2017 Feb.
[Is] ISSN:1432-1335
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Liposarcomas are the most common soft tissue sarcomas of adults. The identification of lipoblastic cells in soft tissue sarcomas is mandatory for the diagnosis of most subtypes of liposarcomas but may be difficult in conventional histology. The present study focuses on the expression and possible diagnostic impact of two PAT family proteins, perilipin 1/perilipin and perilipin 2/adipophilin in human liposarcomas. METHODS: Eighty-seven cases of liposarcomas and 30 cases of non-lipomatous sarcomas were investigated immunohistochemically for perilipin 1 and 2 using entire tissue sections. Statistical analyses were performed using appropriate tests. RESULTS: Most liposarcomas and non-lipomatous sarcomas displayed positivity for perilipin 2. In contrast, while more than two-thirds of liposarcomas presented perilipin 1 positivity, all non-lipomatous sarcomas studied were negative for this marker, with statistical significance (p < 0.001). Perilipin 1 expression increased with adipocytic differentiation of liposarcoma subtypes showing statistical significance (p < 0.001). Non-lipomatous sarcomas demonstrated variable expression levels of perilipin 2. The expression level of perilipin 2 appeared to be correlated with tumor cell degeneration, e.g., through hypoxia. CONCLUSIONS: Perilipin 2 is not well suitable for distinction between liposarcomas and non-lipomatous sarcomas. However, perilipin 1 appeared to be a highly specific marker for liposarcoma and adipocytic differentiation in sarcomas with intermediate sensitivity.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Lipossarcoma/metabolismo
Perilipina-1/metabolismo
[Mh] Termos MeSH secundário: Adipócitos
Diferenciação Celular
Seres Humanos
Perilipina-2/metabolismo
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (PLIN1 protein, human); 0 (PLIN2 protein, human); 0 (Perilipin-1); 0 (Perilipin-2)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE
[do] DOI:10.1007/s00432-016-2263-8


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[PMID]:27591428
[Au] Autor:El-Ekiaby NM; Mekky RY; Riad SE; Elhelw DS; El-Sayed M; Esmat G; Abdelaziz AI
[Ad] Endereço:Department of Pharmacology and Toxicology, Molecular Pathology Research Group, German University in Cairo, New Cairo City-Main Entrance Al Tagamoa Al Khames, Cairo, Egypt.
[Ti] Título:miR-148a and miR-30a limit HCV-dependent suppression of the lipid droplet protein, ADRP, in HCV infected cell models.
[So] Source:J Med Virol;89(4):653-659, 2017 Apr.
[Is] ISSN:1096-9071
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hepatitis C Virus (HCV) promotes lipid droplet (LD) formation and perturbs the expression of the LD associated PAT proteins ADRP and TIP47, to promote its own lifecycle. HCV enhances TIP47 and suppresses ADRP by displacing it from LD surface in infected cell models. We have previously shown that suppression of TIP47 by miR-148a and miR-30a decreased intracellular LDs and HCV RNA. Thus, this study aimed at examining whether this microRNA-mediated suppression of HCV would limit HCV-dependent displacement of ADRP from LDs. ADRP expression was examined in 21 HCV-infected liver biopsies and 9 healthy donor liver tissues as well as in HCV-infected Huh7 cells using qRT-PCR. miR-148a and miR-30a expression was manipulated using specific oligos in JFH-1 infected, oleic acid treated cells, to study their impact on ADRP expression using qRT-PCR, and immunofluorescence microscopy. Intracellular HCV RNA was assessed using qRT-PCR. ADRP is down regulated in patients as well as HCVcc-JFH-I infected cell models. Forcing the expression of both miRNAs induced ADRP on the mRNA and protein levels. This study shows that HCV suppresses hepatic ADRP expression in infected patients and cell lines. Forcing the expression of miR-148a and miR-30a limits the suppressive effect of HCV on ADRP. J. Med. Virol. 89:653-659, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Hepacivirus/patogenicidade
Interações Hospedeiro-Patógeno
MicroRNAs/metabolismo
Perilipina-2/antagonistas & inibidores
[Mh] Termos MeSH secundário: Adulto
Biópsia
Linhagem Celular
Feminino
Perfilação da Expressão Gênica
Hepatite C/patologia
Hepatócitos/virologia
Seres Humanos
Fígado/patologia
Masculino
Microscopia de Fluorescência
Meia-Idade
Perilipina-3/metabolismo
RNA Viral/análise
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN148 microRNA, human); 0 (MIRN30 microRNA, human); 0 (MicroRNAs); 0 (PLIN2 protein, human); 0 (PLIN3 protein, human); 0 (Perilipin-2); 0 (Perilipin-3); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160904
[St] Status:MEDLINE
[do] DOI:10.1002/jmv.24677


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[PMID]:27467545
[Au] Autor:Fujimoto M; Yoshizawa A; Sumiyoshi S; Sonobe M; Menju T; Hirata M; Momose M; Date H; Haga H
[Ad] Endereço:Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto, Japan.
[Ti] Título:Adipophilin expression in lung adenocarcinoma is associated with apocrine-like features and poor clinical prognosis: an immunohistochemical study of 328 cases.
[So] Source:Histopathology;70(2):232-241, 2017 Jan.
[Is] ISSN:1365-2559
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The lipogenic pathway is up-regulated in proliferating cells. However, the clinical impact of neoplastic steatogenesis in lung cancer is unclear. The aim of the present study was to evaluate the association of intracytoplasmic lipids with the clinicopathological features of lung adenocarcinoma (ADC), by immunohistochemical analysis of adipophilin (ADP), a coating protein found on intracytoplasmic lipid droplets. METHODS AND RESULTS: Tissue microarrays consisting of 328 primary lung ADCs surgically resected at Kyoto University Hospital were immunostained for ADP. Subsequently, correlations between ADP expression and clinical, molecular and survival data were performed. Fifty-one (15.5%) cases were ADP-positive. The presence of vascular invasion (P = 0.003), predominantly solid histology (P < 0.001), poorly differentiated type (P < 0.001), wild-type EGFR (P = 0.002), ALK fusion (P < 0.001), strong/diffuse mitochondrial staining (P < 0.001), a lack of surfactant protein B expression (P = 0.014) and a high Ki67 index (P < 0.001) were significantly correlated with ADP-positive ADC. In contrast, there were no correlations between ADP-positive ADC and sex, age, smoking history, tumour stage, thyroid transcription factor-1 expression, or KRAS mutational status. ADP-positive ADCs had apocrine-like features (P < 0.001). Patients with ADP-positive ADC had worse disease-free and overall survival (P = 0.047 and P = 0.013, respectively) than those with ADP-negative ADC. CONCLUSIONS: ADP was expressed in a small proportion of lung ADCs. ADP-positive lung ADC was significantly associated with apocrine-like features, wild-type EGFR, and poor prognosis, suggesting that ADP-positive lung ADC could be a distinct subtype of lung adenocarcinoma, induced by up-regulation of the lipogenic pathway.
[Mh] Termos MeSH primário: Adenocarcinoma/patologia
Biomarcadores Tumorais/análise
Neoplasias Pulmonares/patologia
Perilipina-2/biossíntese
[Mh] Termos MeSH secundário: Adenocarcinoma/mortalidade
Adulto
Idoso
Idoso de 80 Anos ou mais
Intervalo Livre de Doença
Feminino
Seres Humanos
Imuno-Histoquímica
Estimativa de Kaplan-Meier
Neoplasias Pulmonares/mortalidade
Masculino
Meia-Idade
Perilipina-2/análise
Prognóstico
Modelos de Riscos Proporcionais
Análise Serial de Tecidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (PLIN2 protein, human); 0 (Perilipin-2)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160729
[St] Status:MEDLINE
[do] DOI:10.1111/his.13048



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