Base de dados : MEDLINE
Pesquisa : D12.776.157.530.100.228 [Categoria DeCS]
Referências encontradas : 71 [refinar]
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[PMID]:28486145
[Au] Autor:Ren X; Wang Z; Gao B; Liu P; Li J
[Ad] Endereço:Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laborat
[Ti] Título:Effects of florfenicol on the antioxidant status, detoxification system and biomolecule damage in the swimming crab (Portunus trituberculatus).
[So] Source:Ecotoxicol Environ Saf;143:6-11, 2017 Sep.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Florfenicol (FLR) is the most commonly used antibacterial agent in aquaculture because of its wide spectrum of activity and few side-effects. We characterized the toxicokinetics of FLR in the swimming crab (Portunus trituberculatus) after intravenous (IV) dosing (20, 40 and 80mg/kg). The results showed that FLR significantly suppressed the antioxidant system of the hepatopancreas. FLR induced transcriptional expression of phase I and phase II detoxification genes (CYP3 and GST, respectively) in a dose- and clearance time-dependent manner and altered the expression of their corresponding enzymes (erythromycin N-demethylase and glutathione S-transferase, respectively). Moreover, FLR induced the transcription of ATP-binding cassette (ABC) transporter subfamily B (ABCB) and subfamily G (ABCG), although ABCG transcription was not induced by FLR at 20mg/kg. Additionally, higher FLR doses caused significant biomolecule damage during the first 48h after delivery. This study will provide an improved understanding of the exact mechanism underlying toxicity in aquatic organisms.
[Mh] Termos MeSH primário: Antibacterianos/toxicidade
Antioxidantes/metabolismo
Braquiúros/efeitos dos fármacos
Braquiúros/enzimologia
Dano ao DNA
Hepatopâncreas/efeitos dos fármacos
Tianfenicol/análogos & derivados
[Mh] Termos MeSH secundário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Animais
Aquicultura
Braquiúros/genética
Citocromo P-450 CYP3A/genética
Citocromo P-450 CYP3A/metabolismo
Relação Dose-Resposta a Droga
Glutationa Transferase/genética
Glutationa Transferase/metabolismo
Hepatopâncreas/enzimologia
Inativação Metabólica
Peroxidação de Lipídeos/efeitos dos fármacos
Frutos do Mar
Natação
Tianfenicol/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (Anti-Bacterial Agents); 0 (Antioxidants); 9J97307Y1H (florfenicol); EC 1.14.14.1 (Cytochrome P-450 CYP3A); EC 2.5.1.18 (Glutathione Transferase); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE


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[PMID]:28420650
[Au] Autor:Nikolova V; Papacleovoulou G; Bellafante E; Borges Manna L; Jansen E; Baron S; Abu-Hayyeh S; Parker M; Williamson C
[Ad] Endereço:Women's Health Academic Centre, King's College London, London, United Kingdom.
[Ti] Título:Changes in LXR signaling influence early-pregnancy lipogenesis and protect against dysregulated fetoplacental lipid homeostasis.
[So] Source:Am J Physiol Endocrinol Metab;313(4):E463-E472, 2017 Oct 01.
[Is] ISSN:1522-1555
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human pregnancy is associated with enhanced de novo lipogenesis in the early stages followed by hyperlipidemia during advanced gestation. Liver X receptors (LXRs) are oxysterol-activated nuclear receptors that stimulate de novo lipogenesis and also promote the efflux of cholesterol from extrahepatic tissues followed by its transport back to the liver for biliary excretion. Although LXR is recognized as a master regulator of triglyceride and cholesterol homeostasis, it is unknown whether it facilitates the gestational adaptations in lipid metabolism. To address this question, biochemical profiling, protein quantification, and gene expression studies were used, and gestational metabolic changes in T0901317-treated wild-type mice and mutants were investigated. Here, we show that altered LXR signaling contributes to the enhanced lipogenesis in early pregnancy by increasing the expression of hepatic and stearoyl-CoA desaturase 1 ( ). Both the pharmacological activation of LXR with T0901317 and the genetic ablation of its two isoforms disrupted the increase in hepatic fatty acid biosynthesis and the development of hypertriglyceridemia during early gestation. We also demonstrate that absence of LXR enhances maternal white adipose tissue lipolysis, causing abnormal accumulation of triglycerides, cholesterol, and free fatty acids in the fetal liver. Together, these data identify LXR as an important factor in early-pregnancy lipogenesis that is also necessary to protect against abnormalities in fetoplacental lipid homeostasis.
[Mh] Termos MeSH primário: Metabolismo dos Lipídeos
Lipogênese
Receptores X do Fígado/genética
Gravidez/metabolismo
[Mh] Termos MeSH secundário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Animais
Western Blotting
Feminino
Feto/metabolismo
Perfilação da Expressão Gênica
Homeostase
Hidrocarbonetos Fluorados/farmacologia
Receptores X do Fígado/agonistas
Receptores X do Fígado/metabolismo
Proteínas de Membrana Transportadoras/genética
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Placenta/metabolismo
Gravidez/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Estearoil-CoA Dessaturase/genética
Proteína de Ligação a Elemento Regulador de Esterol 1/genética
Sulfonamidas/farmacologia
Receptor fas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (Fas protein, mouse); 0 (Hydrocarbons, Fluorinated); 0 (Liver X Receptors); 0 (Membrane Transport Proteins); 0 (Npc1l1 protein, mouse); 0 (RNA, Messenger); 0 (Srebf1 protein, mouse); 0 (Sterol Regulatory Element Binding Protein 1); 0 (Sulfonamides); 0 (TO-901317); 0 (fas Receptor); EC 1.14.19.1 (Scd1 protein, mouse); EC 1.14.19.1 (Stearoyl-CoA Desaturase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1152/ajpendo.00449.2016


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[PMID]:27968990
[Au] Autor:Chang Z; Chen Z; Yan W; Xie G; Lu J; Wang N; Lu Q; Yao N; Yang G; Xia J; Tang X
[Ad] Endereço:Shenzhen Institute of Molecular Crop Design, Shenzhen 518107, China; Guangdong Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China.
[Ti] Título:An ABC transporter, OsABCG26, is required for anther cuticle and pollen exine formation and pollen-pistil interactions in rice.
[So] Source:Plant Sci;253:21-30, 2016 Dec.
[Is] ISSN:1873-2259
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Wax, cutin and sporopollenin are essential components for the formation of the anther cuticle and the pollen exine, respectively. Their lipid precursors are synthesized by secretory tapetal cells and transported to the anther and microspore surface for deposition. However, the molecular mechanisms involved in the formation of the anther cuticle and pollen exine are poorly understood in rice. Here, we characterized a rice male sterile mutant osabcg26. Molecular cloning and sequence analysis revealed a point mutation in the gene encoding an ATP binding cassette transporter G26 (OsABCG26). OsABCG26 was specifically expressed in the anther and pistil. Cytological analysis revealed defects in tapetal cells, lipidic Ubisch bodies, pollen exine, and anther cuticle in the osabcg26 mutant. Expression of some key genes involved in lipid metabolism and transport, such as UDT1, WDA1, CYP704B2, OsABCG15, OsC4 and OsC6, was significantly altered in osabcg26 anther, possibly due to a disturbance in the homeostasis of anther lipid metabolism and transport. Additionally, wild-type pollen tubes showed a growth defect in osabcg26 pistils, leading to low seed setting in osabcg26 cross-pollinated with the wild-type pollen. These results indicated that OsABCG26 plays an important role in anther cuticle and pollen exine formation and pollen-pistil interactions in rice.
[Mh] Termos MeSH primário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/fisiologia
Oryza/fisiologia
Tubo Polínico/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Flores/ultraestrutura
Dados de Sequência Molecular
Mutação
Oryza/ultraestrutura
Infertilidade das Plantas/genética
Proteínas de Plantas/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (Plant Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE


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[PMID]:27816548
[Au] Autor:Deng L; Wang X; Jiang L; Yang J; Zhou X; Lu Z; Hu H
[Ad] Endereço:Department of Oncology, Shanghai Sixth People's Hospital, Shanghai JiaoTong University, No 600 Yishan Road, Xuhui, Shanghai, 200233, China; Department of Hematology, Zhujiang Hospital, Southern Medical University, No 253 Industrial Avenue, Haizhu, Guangzhou, Guangdong 501282, China.
[Ti] Título:Modulation of miR-185-5p expression by EBV-miR-BART6 contributes to developmental differences in ABCG4 gene expression in human megakaryocytes.
[So] Source:Int J Biochem Cell Biol;81(Pt A):105-111, 2016 Dec.
[Is] ISSN:1878-5875
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Immune thrombocytopenia (ITP) is an acquired autoimmune disorder characterized by low platelet count and bleeding, and is usually triggered by viral infections. We previously reported that 14 viral microRNAs of megakaryocytes cultured with serum from patients with ITP, including ebv-miR-BART6, are up-regulated. Previous research has reported that ebv-miR-BART6 down-regulated the expression of miR-185-5p. We therefore predicted that the ABCG4 gene, which is highly expressed in megakaryocyte progenitor cells, is a direct target of miR-185-5p. We hypothesized that ebv-miR-BART6 may play a role in development and differentiation of megakaryocytes. First, we verified the negative regulation of ABCG4 by miR-185-5p through luciferase assay analysis. Second, after transfection of ebv-miR-BART6 into megakaryocytes developing from normal cord blood mononuclear cells (MNCs), we found that the level of miR-185-5p in the ebv-miR-BART6 group was reduced to almost a third of that in the control groups, accompanied by up-regulation of ABCG4 at both the mRNA and protein levels. Meanwhile, proliferation of megakaryocytes was significantly repressed in the ebv-miR-BART6 group compared with the blank and negative control groups (14.89%±3.13%, 34.15%±2.42% and 30.96%±4.37%, respectively; P<0.001). Our results further revealed that ebv-miR-BART6 inhibited megakaryocyte colony unit formation, decreased CD41 expression and inhibited megakaryocyte polyploidization. These data suggest a new paradigm to explain the mechanisms underlying ITP, involving the regulation of megakaryocytopoiesis by viral microRNAs through the intronic hsa-microRNA.
[Mh] Termos MeSH primário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Regulação da Expressão Gênica/genética
Herpesvirus Humano 4/genética
Megacariócitos/citologia
Megacariócitos/metabolismo
MicroRNAs/genética
RNA Viral/genética
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas/genética
Proliferação Celular/genética
Regulação para Baixo/genética
Feminino
Seres Humanos
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (ABCG4 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (MIRN185 microRNA, human); 0 (MicroRNAs); 0 (RNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:27664094
[Au] Autor:Hou X; Wu W; Yin B; Liu X; Ren F
[Ad] Endereço:The Department of Human Anatomy of Jinzhou Medical University, Jinzhou, Liaoning, China.
[Ti] Título:MicroRNA-463-3p/ABCG4: A new axis in glucose-stimulated insulin secretion.
[So] Source:Obesity (Silver Spring);24(11):2368-2376, 2016 Nov.
[Is] ISSN:1930-739X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Glucose-stimulated insulin secretion (GSIS) is known to be essential in the control of metabolic fuel homeostasis, though the molecular mechanisms involved remain unclear. METHODS: MicroRNA (miRNA)-463-3p and ATP-binding cassette A4 (ABCG4) expression was analyzed by real-time PCR, and the potential role of miRNA-463-3p or ABCG4 was evaluated by overexpressing or silencing such miRNA or genes. RESULTS: The miRNA-463-3p inhibited GSIS without affecting cell viability. Further, mechanistic studies demonstrated that ABCG4 was a direct target of microRNA-463-3p and, to this effect, that ABCG4 played an important role in GSIS. The targeting was relevant in pancreatic islet ß-cells, where GSIS through the miRNA-463-3p/ABCG4 axis was observed. Interestingly, in type 2 diabetes human pancreatic islets, expression of miRNA-463-3p and insulin was upregulated and ABCG4 downregulated compared with nondiabetic controls, and their expression levels were closely correlated. CONCLUSIONS: The findings collectively establish a link between GSIS and the miRNA-463-3p/ABCG4 axis and represent a promising target for future diabetes mellitus treatments.
[Mh] Termos MeSH primário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/fisiologia
Glucose/metabolismo
Insulina/secreção
Ilhotas Pancreáticas/metabolismo
MicroRNAs/fisiologia
[Mh] Termos MeSH secundário: Diabetes Mellitus Tipo 2/metabolismo
Regulação para Baixo
Seres Humanos
Reação em Cadeia da Polimerase em Tempo Real
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCG4 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (Insulin); 0 (MicroRNAs); 0 (microRNA463 microRNA, human); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE
[do] DOI:10.1002/oby.21655


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[PMID]:27271688
[Au] Autor:Yim S; Khare D; Kang J; Hwang JU; Liang W; Martinoia E; Zhang D; Kang B; Lee Y
[Ad] Endereço:Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Korea.
[Ti] Título:Postmeiotic development of pollen surface layers requires two Arabidopsis ABCG-type transporters.
[So] Source:Plant Cell Rep;35(9):1863-73, 2016 Sep.
[Is] ISSN:1432-203X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Two Arabidopsis ABC transporters, ABCG1 and ABCG16, are expressed in the tapetal layer, specifically after postmeiotic microspore release, and play important roles in pollen surface development. The male gametophytic cells of terrestrial plants, the pollen grains, travel far before fertilization, and thus require strong protective layers, which take the form of a pollen coat and a pollen wall. The protective surface structures are generated by the tapetum, the tissue surrounding the developing gametophytes. Many ABC transporters, including Arabidopsis thaliana ABCG1 and ABCG16, have been shown to play essential roles in the development of such protective layers. However, the details of the mechanism of their function remain to be clarified. In this study, we show that ABCG1 and ABCG16 are localized at the plasma membrane of tapetal cells, specifically after postmeiotic microspore release, and play critical roles in the postmeiotic stages of male gametophyte development. Consistent with this stage-specific expression, the abcg1 abcg16 double knockout mutant exhibited defects in pollen development after postmeiotic microspore release; their microspores lacked intact nexine and intine layers, exhibited defects in pollen mitosis I, displayed ectopic deposits of arabinogalactan proteins, failed to complete cytokinesis, and lacked sperm cells. Interestingly, the double mutant exhibited abnormalities in the internal structures of tapetal cells, too; the storage organelles of tapetal cells, tapetosomes and elaioplasts, were morphologically altered. Thus, this work reveals that the lack of ABCG1 and ABCG16 at the tapetal cell membrane causes a broad range of defects in pollen, as well as in tapetal cells themselves. Furthermore, these results suggest that normal pollen surface development is necessary for normal development of the pollen cytoplasm.
[Mh] Termos MeSH primário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Proteínas de Arabidopsis/metabolismo
Arabidopsis/citologia
Arabidopsis/metabolismo
Meiose
Proteínas de Membrana/metabolismo
Pólen/citologia
Pólen/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Arabidopsis/crescimento & desenvolvimento
Arabidopsis/ultraestrutura
Membrana Celular/metabolismo
Parede Celular/metabolismo
Mitose
Mucoproteínas/metabolismo
Mutação/genética
Proteínas de Plantas/metabolismo
Pólen/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCG1 protein, Arabidopsis); 0 (ABCG16 protein, Arabidopsis); 0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (Arabidopsis Proteins); 0 (Membrane Proteins); 0 (Mucoproteins); 0 (Plant Proteins); 0 (arabinogalactan proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE
[do] DOI:10.1007/s00299-016-2001-3


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[PMID]:27228027
[Au] Autor:Hegyi Z; Homolya L
[Ad] Endereço:Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
[Ti] Título:Functional Cooperativity between ABCG4 and ABCG1 Isoforms.
[So] Source:PLoS One;11(5):e0156516, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ABCG4 belongs to the ABCG subfamily, the members of which are half transporters composed of a single transmembrane and a single nucleotide-binding domain. ABCG proteins have a reverse domain topology as compared to other mammalian ABC transporters, and have to form functional dimers, since the catalytic sites for ATP binding and hydrolysis, as well as the transmembrane domains are composed of distinct parts of the monomers. Here we demonstrate that ABCG4 can form homodimers, but also heterodimers with its closest relative, ABCG1. Both the full-length and the short isoforms of ABCG1 can dimerize with ABCG4, whereas the ABCG2 multidrug transporter is unable to form a heterodimer with ABCG4. We also show that contrary to that reported in some previous studies, ABCG4 is predominantly localized to the plasma membrane. While both ABCG1 and ABCG4 have been suggested to be involved in lipid transport or regulation, in accordance with our previous results regarding the long version of ABCG1, here we document that the expression of both the short isoform of ABCG1 as well as ABCG4 induce apoptosis in various cell types. This apoptotic effect, as a functional read-out, allowed us to demonstrate that the dimerization between these half transporters is not only a physical interaction but functional cooperativity. Given that ABCG4 is predominantly expressed in microglial-like cells and endothelial cells in the brain, our finding of ABCG4-induced apoptosis may implicate a new role for this protein in the clearance mechanisms within the central nervous system.
[Mh] Termos MeSH primário: Membro 1 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Apoptose/fisiologia
Membrana Celular/metabolismo
Multimerização Proteica/fisiologia
[Mh] Termos MeSH secundário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Membro 1 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Transporte Biológico Ativo/fisiologia
Encéfalo/citologia
Encéfalo/metabolismo
Membrana Celular/genética
Células Endoteliais/citologia
Células Endoteliais/metabolismo
Células HEK293
Seres Humanos
Microglia/citologia
Microglia/metabolismo
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCG1 protein, human); 0 (ABCG4 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (ATP Binding Cassette Transporter, Sub-Family G, Member 1); 0 (Protein Isoforms)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160527
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0156516


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[PMID]:27196068
[Au] Autor:Sano O; Tsujita M; Shimizu Y; Kato R; Kobayashi A; Kioka N; Remaley AT; Michikawa M; Ueda K; Matsuo M
[Ad] Endereço:Laboratory of Cellular Biochemistry, Division of Applied Life Sciences, Kyoto University Graduate School of Agriculture, Kyoto, 606-8502, Japan.
[Ti] Título:ABCG1 and ABCG4 Suppress γ-Secretase Activity and Amyloid ß Production.
[So] Source:PLoS One;11(5):e0155400, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ATP-binding cassette G1 (ABCG1) and ABCG4, expressed in neurons and glia in the central nervous system, mediate cholesterol efflux to lipid acceptors. The relationship between cholesterol level in the central nervous system and Alzheimer's disease has been reported. In this study, we examined the effects of ABCG1 and ABCG4 on amyloid precursor protein (APP) processing, the product of which, amyloid ß (Aß), is involved in the pathogenesis of Alzheimer's disease. Expression of ABCG1 or ABCG4 in human embryonic kidney 293 cells that stably expressed Swedish-type mutant APP increased cellular and cell surface APP levels. Products of cleavage from APP by α-secretase and by ß-secretase also increased. The levels of secreted Aß, however, decreased in the presence of ABCG1 and ABCG4, but not ABCG4-KM, a nonfunctional Walker-A lysine mutant. In contrast, secreted Aß levels increased in differentiated SH-SY5Y neuron-like cells in which ABCG1 and ABCG4 were suppressed. Furthermore, Aß42 peptide in the cerebrospinal fluid from Abcg1 null mice significantly increased compared to the wild type mice. To examine the underlying mechanism, we analyzed the activity and distribution of γ-secretase. ABCG1 and ABCG4 suppressed γ-secretase activity and disturbed γ-secretase localization in the raft domains where γ-secretase functions. These results suggest that ABCG1 and ABCG4 alter the distribution of γ-secretase on the plasma membrane, leading to the decreased γ-secretase activity and suppressed Aß secretion. ABCG1 and ABCG4 may inhibit the development of Alzheimer's disease and can be targets for the treatment of Alzheimer's disease.
[Mh] Termos MeSH primário: Membro 1 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Secretases da Proteína Precursora do Amiloide/metabolismo
Peptídeos beta-Amiloides/biossíntese
[Mh] Termos MeSH secundário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Membro 1 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Doença de Alzheimer/genética
Doença de Alzheimer/metabolismo
Secretases da Proteína Precursora do Amiloide/genética
Animais
Caveolina 1/metabolismo
Linhagem Celular Tumoral
Membrana Celular/metabolismo
Inativação Gênica
Células HEK293
Seres Humanos
Lisina
Glicoproteínas de Membrana/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Mutação
Interferência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCG1 protein, human); 0 (ABCG4 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (ATP Binding Cassette Transporter, Sub-Family G, Member 1); 0 (Amyloid beta-Peptides); 0 (CAV1 protein, human); 0 (Caveolin 1); 0 (Membrane Glycoproteins); 0 (nicastrin protein); EC 3.4.- (Amyloid Precursor Protein Secretases); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160520
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0155400


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[PMID]:27102667
[Au] Autor:Shibata Y; Ojika M; Sugiyama A; Yazaki K; Jones DA; Kawakita K; Takemoto D
[Ad] Endereço:Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
[Ti] Título:The Full-Size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 Function in Pre- and Postinvasion Defense against Phytophthora infestans in Nicotiana benthamiana.
[So] Source:Plant Cell;28(5):1163-81, 2016 May.
[Is] ISSN:1532-298X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sesquiterpenoid capsidiol is the major phytoalexin produced by Nicotiana and Capsicum species. Capsidiol is produced in plant tissues attacked by pathogens and plays a major role in postinvasion defense by inhibiting pathogen growth. Using virus-induced gene silencing-based screening, we identified two Nicotiana benthamiana (wild tobacco) genes encoding functionally redundant full-size ABCG (PDR-type) transporters, Nb-ABCG1/PDR1 and Nb-ABCG2/PDR2, which are essential for resistance to the potato late blight pathogen Phytophthora infestans Silencing of Nb-ABCG1/2 compromised secretion of capsidiol, revealing Nb-ABCG1/2 as probable exporters of capsidiol. Accumulation of plasma membrane-localized Nb-ABCG1 and Nb-ABCG2 was observed at the site of pathogen penetration. Silencing of EAS (encoding 5-epi-aristolochene synthase), a gene for capsidiol biosynthesis, reduced resistance to P. infestans, but penetration by P. infestans was not affected. By contrast, Nb-ABCG1/2-silenced plants showed reduced penetration defense, indicating that Nb-ABCG1/2 are involved in preinvasion defense against P. infestans Plastidic GGPPS1 (geranylgeranyl diphosphate synthase) was also found to be required for preinvasion defense, thereby suggesting that plastid-produced diterpene(s) are the antimicrobial compounds active in preinvasion defense. These findings suggest that N. benthamiana ABCG1/2 are involved in the export of both antimicrobial diterpene(s) for preinvasion defense and capsidiol for postinvasion defense against P. infestans.
[Mh] Termos MeSH primário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Phytophthora infestans/patogenicidade
Proteínas de Plantas/metabolismo
Tabaco/metabolismo
Tabaco/microbiologia
[Mh] Termos MeSH secundário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Regulação da Expressão Gênica de Plantas/genética
Doenças das Plantas/genética
Doenças das Plantas/microbiologia
Proteínas de Plantas/genética
Tabaco/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (Plant Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160423
[St] Status:MEDLINE
[do] DOI:10.1105/tpc.15.00721


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[PMID]:26906115
[Au] Autor:Zhao G; Shi J; Liang W; Zhang D
[Ad] Endereço:a Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University (SJTU)-University of Adelaide Joint Center for Agriculture and Health, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Sh
[Ti] Título:ATP binding cassette G transporters and plant male reproduction.
[So] Source:Plant Signal Behav;11(3):e1136764, 2016.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The function of ATP Binding Cassette G (ABCG) transporters in the regulation of plant vegetative organs development has been well characterized in various plant species. In contrast, their function in reproductive development particularly male reproductive development received considerably less attention till some ABCG transporters was reported to be associated with anther and pollen wall development in Arabidopsis thaliana and rice (Oryza sativa) during the past decade. This mini-review summarizes current knowledge of ABCG transporters regarding to their roles in male reproduction and underlying genetic and biochemical mechanisms, which makes it evident that ABCG transporters represent one of those conserved and divergent components closely related to male reproduction in plants. This mini-review also discusses the current challenges and future perspectives in this particular field.
[Mh] Termos MeSH primário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/fisiologia
Arabidopsis/fisiologia
Oryza/fisiologia
[Mh] Termos MeSH secundário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Arabidopsis/genética
Arabidopsis/metabolismo
Flores/genética
Flores/crescimento & desenvolvimento
Flores/metabolismo
Regulação da Expressão Gênica de Plantas
Modelos Biológicos
Oryza/genética
Oryza/metabolismo
Pólen/genética
Pólen/crescimento & desenvolvimento
Pólen/metabolismo
Reprodução/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family G)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.1080/15592324.2015.1136764



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