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[PMID]: | 28381288 |
[Au] Autor: | Adibi JJ; Buckley JP; Lee MK; Williams PL; Just AC; Zhao Y; Bhat HK; Whyatt RM |
[Ad] Endereço: | Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, 130 Desoto Street, Parran Hall 5132, Pittsburgh, PA, 15261, USA. adibij@pitt.edu. |
[Ti] Título: | Maternal urinary phthalates and sex-specific placental mRNA levels in an urban birth cohort. |
[So] Source: | Environ Health;16(1):35, 2017 Apr 05. | [Is] ISSN: | 1476-069X |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: Prenatal urinary concentrations of phthalates in women participants in an urban birth cohort were associated with outcomes in their children related to neurodevelopment, autoimmune disease risk, and fat mass at 3,5,7, and 8 years of life. Placental biomarkers and outcomes at birth may offer biologic insight into these associations. This is the first study to address these associations with candidate genes from the phthalate and placenta literature, accounting for sex differences, and using absolute quantitation methods for mRNA levels. METHODS: We measured candidate mRNAs in 180 placentas sampled at birth (HSD17B1, AHR, CGA, CYP19A1, SLC27A4, PTGS2, PPARG, CYP11A1) by quantitative PCR and an absolute standard curve. We estimated associations of log mRNA with quartiles of urinary phthalate monoesters using linear mixed models. Phthalate metabolites (N = 358) and mRNAs (N = 180) were transformed to a z-score and modeled as independent, correlated vectors in relation to large for gestational age (LGA) and gestational diabetes mellitus (GDM). RESULTS: CGA was associated with 4 out of 6 urinary phthalates. CGA was 2.0 log units lower at the 3 vs. 1 quartile of mono-n-butyl phthalate (MnBP) (95% confidence interval (CI): -3.5, -0.5) in male placentas, but 0.6 log units higher (95% CI: -0.8, 1.9) in female placentas (sex interaction p = 0.01). There was an inverse association of MnBP with PPARG in male placentas (-1.1 log units at highest vs. lowest quartile, 95% CI: -2.0, -0.1). CY19A1, CYP11A1, CGA were associated with one or more of the following in a sex-specific manner: monobenzyl phthalate (MBzP), MnBP, mono-iso-butyl phthalate (MiBP). These 3 mRNAs were lower by 1.4-fold (95% CI: -2.4, -1.0) in male GDM placentas vs. female and non-GDM placentas (p-value for interaction = 0.04). The metabolites MnBP/MiBP were 16% higher (95% CI: 0, 22) in GDM pregnancies. CONCLUSIONS: Prenatal concentrations of certain phthalates and outcomes at birth were modestly associated with molecular changes in fetal placental tissue during pregnancy. Associations were stronger in male vs. female placentas, and associations with MnBP and MiBP were stronger than other metabolites. Placental mRNAs are being pursued further as potential mediators of exposure-induced risks to the health of the child. |
[Mh] Termos MeSH primário: |
Poluentes Ambientais/urina Exposição Materna Ácidos Ftálicos/urina Placenta/metabolismo RNA Mensageiro/metabolismo
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[Mh] Termos MeSH secundário: |
Adulto Aromatase/genética Enzima de Clivagem da Cadeia Lateral do Colesterol/genética Estradiol Desidrogenases/genética Proteínas de Transporte de Ácido Graxo/genética Feminino Expressão Gênica Subunidade alfa de Hormônios Glicoproteicos/genética Seres Humanos Masculino PPAR gama/genética Gravidez Receptores de Hidrocarboneto Arílico/genética Caracteres Sexuais População Urbana Adulto Jovem
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Environmental Pollutants); 0 (Fatty Acid Transport Proteins); 0 (Glycoprotein Hormones, alpha Subunit); 0 (PPAR gamma); 0 (Phthalic Acids); 0 (RNA, Messenger); 0 (Receptors, Aryl Hydrocarbon); 0 (SLC27A4 protein, human); EC 1.1.1.62 (Estradiol Dehydrogenases); EC 1.1.1.62 (HSD17B1 protein, human); EC 1.14.14.1 (Aromatase); EC 1.14.14.1 (CYP19A1 protein, human); EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171003 |
[Lr] Data última revisão:
| 171003 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170407 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1186/s12940-017-0241-5 |
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