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Pesquisa : D12.776.157.530.400.150.585.792 [Categoria DeCS]
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[PMID]:28882644
[Au] Autor:Weon H; Kim TW; Youn DH
[Ad] Endereço:Department of Oral Physiology, BioCure Laboratory, School of Dentistry, Kyungpook National University, 2177 Dalgubeol Blvd, Jung-gu, Daegu 41940, Republic of Korea.
[Ti] Título:Postsynaptic N-type or P/Q-type calcium channels mediate long-term potentiation by group I metabotropic glutamate receptors in the trigeminal oralis.
[So] Source:Life Sci;188:110-117, 2017 Nov 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Both N-type and P/Q-type voltage-gated Ca channels (VGCCs) are involved in the induction of long-term potentiation (LTP), the long-lasting increase of synaptic strength, in the central nervous system. To provide further information on the roles of N-type and P/Q-type VGCCs in the induction of LTP at excitatory synapses of trigeminal primary afferents in the spinal trigeminal subnucleus oralis (Vo), we investigated whether they contribute to the induction of LTP by activation of group I metabotropic glutamate receptors (mGluRs). MAIN METHODS: (S)-3,5-Dihydroxyphenylglycine (DHPG; 10µM for 5min), the group I mGluR agonist, was used to induce LTP of excitatory postsynaptic currents that were evoked in the Vo neurons by stimulating the trigeminal track. KEY FINDINGS: Weak blockade of the N-type or P/Q-type VGCCs by ω-conotoxin GVIA or ω-agatoxin IVA, respectively, which inhibited only 20-40% of Ca currents recorded in isolated trigeminal ganglion neurons but had no effect on the basal excitatory synaptic transmission, completely blocked the induction of LTP. In contrast, stronger blockade of the channels, which inhibited >50% of Ca currents and about 30% of basal synaptic transmission, resulted in the development of long-term depression (LTD), the long-lasting decrease of synaptic strength. Interestingly, the postsynaptic mechanism of DHPG-induced LTP, which was determined by paired-pulse ratio, disappeared when LTP was blocked, or LTD occurred, while a presynaptic mechanism still remained. SIGNIFICANCE: Our data suggest that postsynaptic N-type and P/Q-type VGCCs mediate the DHPG-induced LTP at the trigeminal afferent synapses in the Vo.
[Mh] Termos MeSH primário: Canais de Cálcio Tipo N/fisiologia
Canais de Cálcio Tipo P/fisiologia
Canais de Cálcio Tipo Q/fisiologia
Potenciação de Longa Duração/fisiologia
Receptores de Glutamato Metabotrópico/fisiologia
Núcleo Espinal do Trigêmeo/fisiologia
[Mh] Termos MeSH secundário: Agatoxinas/farmacologia
Animais
Agonistas dos Canais de Cálcio/farmacologia
Bloqueadores dos Canais de Cálcio
Cromonas/farmacologia
Feminino
Potenciação de Longa Duração/efeitos dos fármacos
Depressão Sináptica de Longo Prazo/efeitos dos fármacos
Depressão Sináptica de Longo Prazo/fisiologia
Masculino
Terminações Pré-Sinápticas/fisiologia
Ratos
Receptores de Glutamato Metabotrópico/agonistas
Potenciais Sinápticos/fisiologia
Transmissão Sináptica/efeitos dos fármacos
Núcleo Espinal do Trigêmeo/efeitos dos fármacos
ômega-Conotoxinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 ((S)-3,7-dihydroxychroman-4-one); 0 (Agatoxins); 0 (Calcium Channel Agonists); 0 (Calcium Channel Blockers); 0 (Calcium Channels, N-Type); 0 (Calcium Channels, P-Type); 0 (Calcium Channels, Q-Type); 0 (Chromones); 0 (Receptors, Metabotropic Glutamate); 0 (omega-Conotoxins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE


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[PMID]:27702646
[Au] Autor:Martin-Cortecero J; Nuñez A
[Ad] Endereço:Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
[Ti] Título:Sensory responses in the medial prefrontal cortex of anesthetized rats. Implications for sensory processing.
[So] Source:Neuroscience;339:109-123, 2016 Dec 17.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The medial prefrontal cortex (mPFC) plays a key role in higher functions such as memory and attention. In order to demonstrate sensory responses in the mPFC, we used electrophysiological recordings of urethane-anesthetized rats to record somatosensory-evoked potentials (SEPs) or auditory-evoked potentials (AEPs) elicited by whisker deflections and click stimulation, respectively. Contralateral whisker stimulation or auditory stimuli were also applied to study sensory interference in the mPFC. Interference with other sensory stimuli or recent stimulation history reduced whisker responses in the infralimbic and prelimbic cortices of the ventral mPFC. This effect could be mediated by activation of parvalbumin (PV) interneurons since the effect was blocked by the P/Q calcium channel antagonist ω-agatoxin. In contrast, sensory interference or the recent stimulation history was not detected by the dorsal mPFC or the primary somatosensory cortex. Results obtained from retrograde tracer injections in the dorsal and ventral regions of the mPFC indicated that somatosensory and auditory sensory inputs may arrive at the dorsal mPFC through secondary sensory cortical areas, and through the insular and temporal cortical areas. The ventral mPFC may receive sensory information through the strong anatomical connections between the dorsal and ventral mPFC areas. In conclusion, results suggest mPFC plays an important role in sensory processing, which may have important implications in attentional and memory processes.
[Mh] Termos MeSH primário: Percepção Auditiva/fisiologia
Córtex Pré-Frontal/fisiologia
Percepção do Tato/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica/efeitos dos fármacos
Adaptação Fisiológica/fisiologia
Agatoxinas/farmacologia
Anestésicos Intravenosos/farmacologia
Animais
Percepção Auditiva/efeitos dos fármacos
Bloqueadores dos Canais de Cálcio/farmacologia
Canais de Cálcio Tipo P/metabolismo
Canais de Cálcio Tipo Q/metabolismo
Potenciais Evocados Auditivos/efeitos dos fármacos
Potenciais Evocados Auditivos/fisiologia
Potenciais Somatossensoriais Evocados/efeitos dos fármacos
Potenciais Somatossensoriais Evocados/fisiologia
Feminino
Masculino
Neurônios/citologia
Neurônios/efeitos dos fármacos
Neurônios/metabolismo
Córtex Pré-Frontal/citologia
Córtex Pré-Frontal/efeitos dos fármacos
Ratos Sprague-Dawley
Percepção do Tato/efeitos dos fármacos
Uretana/farmacologia
Vibrissas/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Agatoxins); 0 (Anesthetics, Intravenous); 0 (Calcium Channel Blockers); 0 (Calcium Channels, P-Type); 0 (Calcium Channels, Q-Type); 3IN71E75Z5 (Urethane)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


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[PMID]:27586650
[Au] Autor:Rodriguez-Tapia E; Perez-Medina A; Bian X; Galligan JJ
[Ad] Endereço:The Neuroscience Program, Michigan State University, East Lansing, Michigan; and.
[Ti] Título:Upregulation of L-type calcium channels in colonic inhibitory motoneurons of P/Q-type calcium channel-deficient mice.
[So] Source:Am J Physiol Gastrointest Liver Physiol;311(4):G763-G774, 2016 Oct 01.
[Is] ISSN:1522-1547
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enteric inhibitory motoneurons use nitric oxide and a purine neurotransmitter to relax gastrointestinal smooth muscle. Enteric P/Q-type Ca channels contribute to excitatory neuromuscular transmission; their contribution to inhibitory transmission is less clear. We used the colon from tottering mice (tg/tg, loss of function mutation in the α1A pore-forming subunit of P/Q-type Ca channels) to test the hypothesis that P/Q-type Ca channels contribute to inhibitory neuromuscular transmission and colonic propulsive motility. Fecal pellet output in vivo and the colonic migrating motor complex (ex vivo) were measured. Neurogenic circular muscle relaxations and inhibitory junction potentials (IJPs) were also measured ex vivo. Colonic propulsive motility in vivo and ex vivo was impaired in tg/tg mice. IJPs were either unchanged or somewhat larger in tissues from tg/tg compared with wild-type (WT) mice. Nifedipine (L-type Ca channel antagonist) inhibited IJPs by 35 and 14% in tissues from tg/tg and WT mice, respectively. The contribution of N- and R-type channels to neuromuscular transmission was larger in tissues from tg/tg compared with WT mice. The resting membrane potential of circular muscle cells was similar in tissues from tg/tg and WT mice. Neurogenic relaxations of circular muscle from tg/tg and WT mice were similar. These results demonstrate that a functional deficit in P/Q-type channels does not alter propulsive colonic motility. Myenteric neuron L-type Ca channel function increases to compensate for loss of functional P/Q-type Ca channels. This compensation maintains inhibitory neuromuscular transmission and normal colonic motility.
[Mh] Termos MeSH primário: Canais de Cálcio Tipo L/metabolismo
Canais de Cálcio Tipo P/metabolismo
Canais de Cálcio Tipo Q/metabolismo
Colo/inervação
Neurônios Motores/metabolismo
Regulação para Cima
[Mh] Termos MeSH secundário: Animais
Canais de Cálcio Tipo L/genética
Canais de Cálcio Tipo P/genética
Canais de Cálcio Tipo Q/genética
Motilidade Gastrointestinal/fisiologia
Potenciais da Membrana/fisiologia
Camundongos
Camundongos Knockout
Músculo Liso/fisiologia
Transmissão Sináptica/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Channels, L-Type); 0 (Calcium Channels, P-Type); 0 (Calcium Channels, Q-Type)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171001
[Lr] Data última revisão:
171001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160903
[St] Status:MEDLINE
[do] DOI:10.1152/ajpgi.00263.2016


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[PMID]:27475316
[Au] Autor:Kaur D; Raheja D
[Ad] Endereço:Department of Neurology, Penn State Hershey Medical Center, EC 037, Hershey, PA 17033, USA.
[Ti] Título:A case of encephalomyeloradiculopathy in a non-carcinomatous patient associated with P/Q type voltage gated calcium channel antibodies.
[So] Source:J Clin Neurosci;33:232-233, 2016 Nov.
[Is] ISSN:1532-2653
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:The presence of P/Q type voltage gated calcium channel (VGCC) antibodies has been strongly correlated with Lambert Eaton Syndrome (LES), present in 90% of non-immunocompromised patients with LES. However, there have been case reports which have shown its association between paraneoplastic syndrome affecting both central nervous system and the peripheral nervous system causing encephalomyelitis and sensory neuronopathy/neuropathy. We present a case of a young man, who presented with encephalomyelitis, and was further noted to have superimposed cervical polyradiculopathy associated with P/Q type VGCC antibodies.
[Mh] Termos MeSH primário: Autoanticorpos/imunologia
Canais de Cálcio Tipo P/imunologia
Canais de Cálcio Tipo Q/imunologia
Encefalomielite/imunologia
Polirradiculopatia/imunologia
[Mh] Termos MeSH secundário: Autoanticorpos/sangue
Encefalomielite/complicações
Seres Humanos
Masculino
Polirradiculopatia/complicações
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autoantibodies); 0 (Calcium Channels, P-Type); 0 (Calcium Channels, Q-Type)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170210
[Lr] Data última revisão:
170210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160801
[St] Status:MEDLINE


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[PMID]:27349310
[Au] Autor:Antoniotti S; Ruffinatti FA; Torriano S; Luganini A; D'Alessandro R; Lovisolo D
[Ad] Endereço:Department of Life Sciences and Systems Biology, University of Torino, via Accademia Albertina 13, 10123, Torino, Italy; NIS Centre for Nanostructured Interfaces and Surfaces, University of Torino, Italy.
[Ti] Título:REST levels affect the functional expression of voltage dependent calcium channels and the migratory activity in immortalized GnRH neurons.
[So] Source:Neurosci Lett;629:19-25, 2016 08 26.
[Is] ISSN:1872-7972
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The repressor element-1 silencing transcription factor (REST) has emerged as a key controller of neuronal differentiation and has been shown to play a critical role in the expression of the neuronal phenotype; however, much has still to be learned about its role at specific developmental stages and about the functional targets affected. Among these targets, calcium signaling mechanisms are critically dependent on the developmental stage and their full expression is a hallmark of the mature, functional neuron. We have analyzed the role played by REST in GN11 cells, an immortalized cell line derived from gonadotropin hormone releasing hormone (GnRH) neurons at an early developmental stage, electrically non-excitable and with a strong migratory activity. We show for the first time that functional voltage-dependent calcium channels are expressed in wild type GN11 cells; down-regulation of REST by a silencing approach shifts these cells towards a more differentiated phenotype, increasing the functional expression of P/Q-type channels and reducing their migratory potential.
[Mh] Termos MeSH primário: Canais de Cálcio Tipo P/metabolismo
Canais de Cálcio Tipo Q/metabolismo
Movimento Celular
Hormônio Liberador de Gonadotropina/metabolismo
Neurônios/metabolismo
Neurônios/fisiologia
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Animais
Sinalização do Cálcio
Técnicas de Cultura de Células
Proliferação Celular
Técnicas de Silenciamento de Genes
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Calcium Channels, P-Type); 0 (Calcium Channels, Q-Type); 0 (RE1-silencing transcription factor); 0 (Repressor Proteins); 33515-09-2 (Gonadotropin-Releasing Hormone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160629
[St] Status:MEDLINE


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[PMID]:26235044
[Au] Autor:Xiong D; Wang Y; Tang C; Fang Y; Zou J; Tian C
[Ad] Endereço:The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, China.
[Ti] Título:VdCrz1 is involved in microsclerotia formation and required for full virulence in Verticillium dahliae.
[So] Source:Fungal Genet Biol;82:201-12, 2015 Sep.
[Is] ISSN:1096-0937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Calcium signaling plays crucial roles in ion stress tolerance, sporulation and pathogenicity in fungi. Although the signaling pathway mediated by calcineurin and the calcineurin-responsive zinc finger transcription factor Crz1 is well characterized in other fungi, this pathway is not well characterized in the phytopathogenic fungus, Verticillium dahliae. To better understand the role of this calcineurin-dependent transcription factor in V. dahliae, an ortholog of CRZ1, VdCrz1, was identified and characterized functionally. Transcriptional analysis of VdCrz1 and GFP expression driven by the VdCrz1 promoter indicated that VdCrz1 was involved in microsclerotia development. After targeted deletion of VdCrz1, microsclerotia formation and melanin accumulation were impaired. Furthermore, the ΔVdCrz1 mutants were hypersensitive to high concentrations of Ca(2+) and cell wall-perturbing agents, such as sodium dodecyl sulfate. The addition of Mg(2+) to the medium restores the microsclerotia formation in ΔVdCrz1 mutants. The ΔVdCrz1 mutants exhibited delayed Verticillium wilt symptoms on smoke tree. These results suggest that VdCrz1 plays important roles in Ca(2+) signaling, cell wall integrity, microsclerotia development and full virulence in V. dahliae.
[Mh] Termos MeSH primário: Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Verticillium/genética
Verticillium/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Canais de Cálcio Tipo P/genética
Canais de Cálcio Tipo P/metabolismo
Núcleo Celular/metabolismo
Proteínas Fúngicas/química
Perfilação da Expressão Gênica
Regulação Fúngica da Expressão Gênica
Dados de Sequência Molecular
Mutação
Fenótipo
Filogenia
Doenças das Plantas/microbiologia
Transporte Proteico
Tolerância a Sal/genética
Alinhamento de Sequência
Fatores de Transcrição/genética
Verticillium/patogenicidade
Virulência/genética
Dedos de Zinco/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Calcium Channels, P-Type); 0 (Fungal Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150831
[Lr] Data última revisão:
150831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150804
[St] Status:MEDLINE


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[PMID]:25975857
[Au] Autor:Kruse JL; Lapid MI; Lennon VA; Klein CJ; Toole OO; Pittock SJ; Strand EA; Frye MA; McKeon A
[Ad] Endereço:Department of Psychiatry and Psychology, College of Medicine, Mayo Clinic, Rochester, MN.
[Ti] Título:Psychiatric Autoimmunity: N-Methyl-D-Aspartate Receptor IgG and Beyond.
[So] Source:Psychosomatics;56(3):227-41, 2015 May-Jun.
[Is] ISSN:1545-7206
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Descriptions of psychiatric autoimmunity beyond N-methyl-D-aspartate (NMDA) receptor encephalitis are sparse. OBJECTIVE: To report the autoimmune psychiatric spectrum currently recognized in Mayo Clinic practice. METHODS: Medical record review, testing of stored serum and cerebrospinal fluid for IgGs reactive with synaptic receptors and ion channels, neuronal nuclear and cytoplasmic antigens (including glutamic acid decarboxylase 65-kDa isoform) and case-control comparison were conducted. Patients were categorized into group 1, all adult psychiatric inpatients tested for neural autoantibodies (2002-2011; n = 213), and group 2, all Mayo NMDA receptor IgG-positive patients (2009-2013; n = 13); healthy control subjects were also included (n = 173). RESULTS: In group 1, at least 1 serum autoantibody (but not NMDA receptor IgG) was detected in 36 of 213 psychiatric inpatients. In total, 12 patients were determined retrospectively to have high-likelihood autoimmune encephalitic diagnoses. The most commonly detected autoantibody specificities were voltage-gated potassium channel ([Kv1] VGKC) complex (6) and calcium channel (P/Q type or N type; 5). Symptoms seen were as follows: depressive (8), anxious (7), psychotic (7), disorganized (5), suicidal (3), manic (1) and catatonic (1). In group 2, among 13 NMDA receptor IgG-positive patients, 12 had encephalitis; their psychiatric symptoms were as follows: depressive (9), catatonic (9), disorganized (8), anxious (8), psychotic (7), manic (6), and suicidal (3). Catatonic symptoms were more common in the 12 NMDA receptor IgG-positive patients than in the 12 group 1 patients with high likelihood of encephalitis (p = 0.002). Antibody positivities were usually low positive in value among healthy controls (12 of 16 vs 3 of 12 group 1 encephalitis cases, p = 0.025). NMDA receptor IgG was not detected in any healthy control subject. CONCLUSIONS: A spectrum of psychiatric autoimmunity beyond NMDA-R IgG may be under-recognized. Diagnosis is facilitated by combining results of comprehensive psychiatric, laboratory, radiologic, and electrophysiologic evaluations.
[Mh] Termos MeSH primário: Encefalite Antirreceptor de N-Metil-D-Aspartato/imunologia
Autoanticorpos/imunologia
Canais de Cálcio Tipo N/imunologia
Transtornos Mentais/imunologia
Canais de Potássio de Abertura Dependente da Tensão da Membrana/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Encefalite Antirreceptor de N-Metil-D-Aspartato/psicologia
Transtornos de Ansiedade/imunologia
Transtornos de Ansiedade/psicologia
Doenças Autoimunes/imunologia
Doenças Autoimunes/psicologia
Autoimunidade/imunologia
Transtorno Bipolar/imunologia
Transtorno Bipolar/psicologia
Canais de Cálcio Tipo P/imunologia
Canais de Cálcio Tipo Q/imunologia
Estudos de Casos e Controles
Catatonia/imunologia
Catatonia/psicologia
Transtorno Depressivo/imunologia
Transtorno Depressivo/psicologia
Feminino
Seres Humanos
Imunoglobulina G/imunologia
Masculino
Transtornos Mentais/psicologia
Meia-Idade
Transtornos Psicóticos/imunologia
Transtornos Psicóticos/psicologia
Receptores de N-Metil-D-Aspartato/imunologia
Esquizofrenia Hebefrênica/imunologia
Esquizofrenia Hebefrênica/psicologia
Ideação Suicida
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autoantibodies); 0 (Calcium Channels, N-Type); 0 (Calcium Channels, P-Type); 0 (Calcium Channels, Q-Type); 0 (Immunoglobulin G); 0 (Potassium Channels, Voltage-Gated); 0 (Receptors, N-Methyl-D-Aspartate)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150516
[St] Status:MEDLINE


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[PMID]:25707622
[Au] Autor:Zhang Y; Li H; Pu Y; Gong S; Liu C; Jiang X; Tao J
[Ad] Endereço:Department of Neurobiology, Medical College of Soochow University, Suzhou, China; Department of Geriatrics and Institute of Neuroscience, The Second Affiliated Hospital of Soochow University, Suzhou, China.
[Ti] Título:Melatonin-mediated inhibition of Purkinje neuron P-type Ca²âº channels in vitro induces neuronal hyperexcitability through the phosphatidylinositol 3-kinase-dependent protein kinase C delta pathway.
[So] Source:J Pineal Res;58(3):321-34, 2015 Apr.
[Is] ISSN:1600-079X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although melatonin receptors are widely expressed in the mammalian central nervous system and peripheral tissues, there are limited data regarding the functions of melatonin in cerebellar Purkinje cells. Here, we identified a novel functional role of melatonin in modulating P-type Ca(2+) channels and action-potential firing in rat Purkinje neurons. Melatonin at 0.1 µm reversibly decreased peak currents (I(Ba)) by 32.9%. This effect was melatonin receptor 1 (MT(R1)) dependent and was associated with a hyperpolarizing shift in the voltage dependence of inactivation. Pertussis toxin pretreatment, intracellular application of QEHA peptide, and a selective antibody raised against the Gß subunit prevented the inhibitory effects of melatonin. Pretreatment with phosphatidylinositol 3-kinase (PI3K) inhibitors abolished the melatonin-induced decrease in I(Ba). Surprisingly, melatonin responses were not regulated by Akt, a common downstream target of PI3K. Melatonin treatment significantly increased protein kinase C (PKC) activity 2.1-fold. Antagonists of PKC, but not of protein kinase A, abolished the melatonin-induced decrease in I(Ba). Melatonin application increased the membrane abundance of PKCδ, and PKCδ inhibition (either pharmacologically or genetically) abolished the melatonin-induced IBa response. Functionally, melatonin increased spontaneous action-potential firing by 53.0%; knockdown of MT(R1) and blockade of P-type channels abolished this effect. Thus, our results suggest that melatonin inhibits P-type channels through MT(R1) activation, which is coupled sequentially to the ßγ subunits of G(i/o)-protein and to downstream PI3K-dependent PKCδ signaling. This likely contributes to its physiological functions, including spontaneous firing of cerebellar Purkinje neurons.
[Mh] Termos MeSH primário: Canais de Cálcio Tipo P/efeitos dos fármacos
Melatonina/farmacologia
Fosfatidilinositol 3-Quinase/metabolismo
Proteína Quinase C-delta/metabolismo
Células de Purkinje/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Canais de Cálcio Tipo P/metabolismo
Células de Purkinje/metabolismo
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Calcium Channels, P-Type); EC 2.7.1.137 (Phosphatidylinositol 3-Kinase); EC 2.7.11.13 (Protein Kinase C-delta); JL5DK93RCL (Melatonin)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150316
[Lr] Data última revisão:
150316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150225
[St] Status:MEDLINE
[do] DOI:10.1111/jpi.12218


  9 / 400 MEDLINE  
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[PMID]:25472955
[Au] Autor:Molina-Campos E; Xu Y; Atchison WD
[Ad] Endereço:Department of Pharmacology and Toxicology (Y.X., W.D.A.) and Genetics Program (E.M.-C, W.D.A.), Michigan State University, East Lansing, Michigan.
[Ti] Título:Age-dependent contribution of P/Q- and R-type Ca2+ channels to neuromuscular transmission in lethargic mice.
[So] Source:J Pharmacol Exp Ther;352(2):395-404, 2015 Feb.
[Is] ISSN:1521-0103
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ß-Subunits of voltage-gated calcium channels (VGCCs) regulate assembly and membrane localization of the pore-forming α1-subunit and strongly influence channel function. ß4-Subunits normally coassociate with α1A-subunits which comprise P/Q-type (Cav2.1) VGCCs. These control acetylcholine (ACh) release at adult mammalian neuromuscular junctions (NMJs). The naturally occurring lethargic (lh) mutation of the ß4-subunit in mice causes loss of the α1-binding site, possibly affecting P/Q-type channel expression or function, and thereby ACh release. End-plate potentials and miniature end-plate potentials were recorded at hemidiaphragm NMJs of 5-7-week and 3-5-month-old lh and wild-type (wt) mice. Sensitivity to antagonists of P/Q- [ω-agatoxin IVA (ω-Aga-IVA)], L- (nimodipine), N- (ω-conotoxin GVIA), and R-type [C192H274N52O60S7 (SNX-482)] VGCCs was compared in juvenile and adult lh and wt mice. Quantal content (m) of adult, but not juvenile, lh mice was reduced compared to wt. ω-Aga-IVA (~60%) and SNX-482 (~ 45%) significantly reduced m in adult lh mice. Only Aga-IVA affected wt adults. In juvenile lh mice, ω-Aga-IVA and SNX-482 decreased m by >75% and ~20%, respectively. Neither ω-conotoxin GVIA nor nimodipine affected ACh release in any group. Immunolabeling revealed α1E and α1A, ß1, and ß3 staining at adult lh, but not wt NMJs. Therefore, in lh mice, when the ß-subunit that normally coassociates with α1A to form P/Q channels is missing, P/Q-type channels partner with other ß-subunits. However, overall participation of P/Q-type channels is reduced and compensated for by R-type channels. R-type VGCC participation is age-dependent, but is less effective than P/Q-type at sustaining NMJ function.
[Mh] Termos MeSH primário: Envelhecimento/metabolismo
Canais de Cálcio Tipo P/metabolismo
Canais de Cálcio Tipo Q/metabolismo
Canais de Cálcio Tipo R/metabolismo
Canais de Cálcio/genética
Junção Neuromuscular/metabolismo
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Potenciais de Ação/efeitos dos fármacos
Envelhecimento/genética
Animais
Bloqueadores dos Canais de Cálcio/farmacologia
Cerebelo/efeitos dos fármacos
Cerebelo/metabolismo
Camundongos Mutantes
Placa Motora/efeitos dos fármacos
Placa Motora/metabolismo
Mutação
Junção Neuromuscular/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Cacnb4 protein, mouse); 0 (Calcium Channel Blockers); 0 (Calcium Channels); 0 (Calcium Channels, P-Type); 0 (Calcium Channels, Q-Type); 0 (Calcium Channels, R-Type); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141205
[St] Status:MEDLINE
[do] DOI:10.1124/jpet.114.216143


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[PMID]:25444432
[Au] Autor:Okada A; Koike H; Nakamura T; Motomura M; Sobue G
[Ad] Endereço:Department of Neurology, Nagoya University Graduate School of Medicine, 65 Tsurumai-Cho, Showa-Ku, Nagoya 466-8550, Japan.
[Ti] Título:Efficacy of intravenous immunoglobulin for treatment of Lambert-Eaton myasthenic syndrome without anti-presynaptic P/Q-type voltage-gated calcium channel antibodies: a case report.
[So] Source:Neuromuscul Disord;25(1):70-2, 2015 Jan.
[Is] ISSN:1873-2364
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We evaluated the efficacy of intravenous immunoglobulin (IVIg) in a patient with Lambert-Eaton myasthenic syndrome (LEMS). Comprehensive clinical and electrophysiological testing was performed on a 34-year-old woman with progressive limb weakness, before and after IVIg treatment. Neurological examination revealed muscle weakness, predominantly in the proximal parts of the limbs. Muscle weakness improved following a short period of maximum voluntary muscle contraction. A repetitive low-rate (3-Hz) nerve stimulation test of the abductor hallucis was normal, but high-rate (20-Hz) stimulation induced an incremental response. Anti-presynaptic P/Q-type voltage-gated calcium channel (P/Q-VGCC) antibodies were absent in the patient's serum. Whole body computed tomography revealed no tumors. We diagnosed seronegative LEMS without tumor and treated the patient with IVIg. Both clinical and electrophysiological indices improved gradually after treatment. This case study indicates that treatment with IVIg is equally effective for LEMS that is seronegative or seropositive for P/Q-VGCC antibodies.
[Mh] Termos MeSH primário: Canais de Cálcio Tipo P/imunologia
Canais de Cálcio Tipo Q/imunologia
Imunoglobulinas Intravenosas/uso terapêutico
Síndrome Miastênica de Lambert-Eaton/diagnóstico
Síndrome Miastênica de Lambert-Eaton/tratamento farmacológico
Síndrome Miastênica de Lambert-Eaton/imunologia
[Mh] Termos MeSH secundário: Administração Intravenosa
Adulto
Autoanticorpos
Canais de Cálcio Tipo P/sangue
Canais de Cálcio Tipo Q/sangue
Feminino
Seres Humanos
Imunoglobulinas Intravenosas/administração & dosagem
Músculo Esquelético/fisiopatologia
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Autoantibodies); 0 (Calcium Channels, P-Type); 0 (Calcium Channels, Q-Type); 0 (Immunoglobulins, Intravenous)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141229
[Lr] Data última revisão:
141229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE



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