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Pesquisa : D12.776.157.530.400.400.500 [Categoria DeCS]
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[PMID]:29032150
[Au] Autor:Sundararajan T; Manzardo AM; Butler MG
[Ad] Endereço:Department of Psychiatry and Behavioral Sciences, University of Kansas Medical Center, Kansas City, KS, United States.
[Ti] Título:Functional analysis of schizophrenia genes using GeneAnalytics program and integrated databases.
[So] Source:Gene;641:25-34, 2018 Jan 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Schizophrenia (SCZ) is a chronic debilitating neuropsychiatric disorder with multiple risk factors involving numerous complex genetic influences. We examined and updated a master list of clinically relevant and susceptibility genes associated with SCZ reported in the literature and genomic databases dedicated to gene discovery for characterization of SCZ genes. We used the commercially available GeneAnalytics computer-based gene analysis program and integrated genomic databases to create a molecular profile of the updated list of 608 SCZ genes to model their impact in select categories (tissues and cells, diseases, pathways, biological processes, molecular functions, phenotypes and compounds) using specialized GeneAnalytics algorithms. Genes for schizophrenia were predominantly expressed in the cerebellum, cerebral cortex, medulla oblongata, thalamus and hypothalamus. Psychiatric/behavioral disorders incorporating SCZ genes included ADHD, bipolar disorder, autism spectrum disorder and alcohol dependence as well as cancer, Alzheimer's and Parkinson's disease, sleep disturbances and inflammation. Function based analysis of major biological pathways and mechanisms associated with SCZ genes identified glutaminergic receptors (e.g., GRIA1, GRIN2, GRIK4, GRM5), serotonergic receptors (e.g., HTR2A, HTR2C), GABAergic receptors (e.g., GABRA1, GABRB2), dopaminergic receptors (e.g., DRD1, DRD2), calcium-related channels (e.g., CACNA1H, CACNA1B), solute transporters (e.g., SLC1A1, SLC6A2) and for neurodevelopment (e.g., ADCY1, MEF2C, NOTCH2, SHANK3). Biological mechanisms involving synaptic transmission, regulation of membrane potential and transmembrane ion transport were identified as leading molecular functions associated with SCZ genes. Our approach to interrogate SCZ genes and their interactions at various levels has increased our knowledge and insight into the disease process possibly opening new avenues for therapeutic intervention.
[Mh] Termos MeSH primário: Estudo de Associação Genômica Ampla
Transporte de Íons/genética
Potenciais da Membrana/genética
Esquizofrenia/genética
Transmissão Sináptica/genética
[Mh] Termos MeSH secundário: Sistemas de Transporte de Aminoácidos/genética
Canais de Cálcio/genética
Cerebelo/citologia
Córtex Cerebral/citologia
Bases de Dados Genéticas
Seres Humanos
Hipotálamo/citologia
Bulbo/citologia
Receptores Dopaminérgicos/genética
Receptores de GABA-A/genética
Receptores Ionotrópicos de Glutamato/genética
Receptores de Serotonina/genética
Tálamo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acid Transport Systems); 0 (Calcium Channels); 0 (Receptors, Dopamine); 0 (Receptors, GABA-A); 0 (Receptors, Ionotropic Glutamate); 0 (Receptors, Serotonin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


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[PMID]:28455372
[Au] Autor:Mallik B; Dwivedi MK; Mushtaq Z; Kumari M; Verma PK; Kumar V
[Ad] Endereço:Department of Biological Sciences, AB-3, Indian Institute of Science Education and Research, Bhauri, Bhopal, Madhya Pradesh 462066, India.
[Ti] Título:Regulation of neuromuscular junction organization by Rab2 and its effector ICA69 in .
[So] Source:Development;144(11):2032-2044, 2017 06 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mechanisms underlying synaptic differentiation, which involves neuronal membrane and cytoskeletal remodeling, are not completely understood. We performed a targeted RNAi-mediated screen of BAR-domain proteins and identified islet cell autoantigen 69 kDa (ICA69) as one of the key regulators of morphological differentiation of the larval neuromuscular junction (NMJ). We show that ICA69 colocalizes with α-Spectrin at the NMJ. The conserved N-BAR domain of ICA69 deforms liposomes Full-length ICA69 and the ICAC but not the N-BAR domain of ICA69 induce filopodia in cultured cells. Consistent with its cytoskeleton regulatory role, mutants show reduced α-Spectrin immunoreactivity at the larval NMJ. Manipulating levels of ICA69 or its interactor PICK1 alters the synaptic level of ionotropic glutamate receptors (iGluRs). Moreover, reducing PICK1 or Rab2 levels phenocopies mutation. Interestingly, Rab2 regulates not only synaptic iGluR but also ICA69 levels. Thus, our data suggest that: (1) ICA69 regulates NMJ organization through a pathway that involves PICK1 and Rab2, and (2) Rab2 functions genetically upstream of ICA69 and regulates NMJ organization and targeting/retention of iGluRs by regulating ICA69 levels.
[Mh] Termos MeSH primário: Autoantígenos/metabolismo
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/metabolismo
Junção Neuromuscular/metabolismo
Proteína rab2 de Ligação ao GTP/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Técnicas de Silenciamento de Genes
Larva/metabolismo
Lipossomos
Mutação/genética
Subunidades Proteicas/metabolismo
Transporte Proteico
Pseudópodes/metabolismo
Interferência de RNA
Receptores Ionotrópicos de Glutamato/metabolismo
Sinapses/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Autoantigens); 0 (Drosophila Proteins); 0 (Liposomes); 0 (Protein Subunits); 0 (Receptors, Ionotropic Glutamate); EC 3.6.5.2 (rab2 GTP-Binding Protein)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1242/dev.145920


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[PMID]:28942923
[Au] Autor:Paul A; Crow M; Raudales R; He M; Gillis J; Huang ZJ
[Ad] Endereço:Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
[Ti] Título:Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity.
[So] Source:Cell;171(3):522-539.e20, 2017 Oct 19.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding the organizational logic of neural circuits requires deciphering the biological basis of neuronal diversity and identity, but there is no consensus on how neuron types should be defined. We analyzed single-cell transcriptomes of a set of anatomically and physiologically characterized cortical GABAergic neurons and conducted a computational genomic screen for transcriptional profiles that distinguish them from one another. We discovered that cardinal GABAergic neuron types are delineated by a transcriptional architecture that encodes their synaptic communication patterns. This architecture comprises 6 categories of ∼40 gene families, including cell-adhesion molecules, transmitter-modulator receptors, ion channels, signaling proteins, neuropeptides and vesicular release components, and transcription factors. Combinatorial expression of select members across families shapes a multi-layered molecular scaffold along the cell membrane that may customize synaptic connectivity patterns and input-output signaling properties. This molecular genetic framework of neuronal identity integrates cell phenotypes along multiple axes and provides a foundation for discovering and classifying neuron types.
[Mh] Termos MeSH primário: Neurônios GABAérgicos/citologia
Perfilação da Expressão Gênica
Análise de Célula Única
[Mh] Termos MeSH secundário: Animais
Moléculas de Adesão Celular Neuronais/metabolismo
Matriz Extracelular/metabolismo
Neurônios GABAérgicos/metabolismo
Camundongos
Receptores de GABA/metabolismo
Receptores Ionotrópicos de Glutamato/metabolismo
Transdução de Sinais
Sinapses
Transcrição Genética
Zinco/metabolismo
Ácido gama-Aminobutírico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Adhesion Molecules, Neuronal); 0 (Receptors, GABA); 0 (Receptors, Ionotropic Glutamate); 56-12-2 (gamma-Aminobutyric Acid); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE


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[PMID]:28912112
[Au] Autor:Wang Y; Chen Q; Guo J; Li J; Wang J; Wen M; Zhao H; Ren B
[Ad] Endereço:Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, Jilin, China; Key Laboratory of Vegetation Ecology, MOE, Northeast Normal University, Changchun, China.
[Ti] Título:Molecular basis of peripheral olfactory sensing during oviposition in the behavior of the parasitic wasp Anastatus japonicus.
[So] Source:Insect Biochem Mol Biol;89:58-70, 2017 Oct.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Anastatus japonicus is a parasitic wasp and natural enemy of the litchi pest Tessaratoma papillosa, and for decades in China, A. japonicus has been mass-reared inside the eggs of Antheraea pernyi to control T. papillosa. A series of experiments was performed to explore the olfactory mechanism underlying the oviposition behavior of A. japonicus. First, a transcriptomic analysis was performed on the antennae of A. japonicus, and the resulting assemblies led to the generation of 70,473 unigenes. Subsequently, 21,368 unigenes were matched to known proteins, 48 odorant receptors (ORs) (including Orco) and 13 antennal ionotropic receptors (IRs) (including the co-receptors IR8a and IR25a) were identified and predicted to form complete open reading frames (ORFs). The FPKM (fragments per Kb per million reads) values and RT-PCR results showed that AjapOrco, AjapOR10, AjapOR27, AjapOR33 and AjapOR35 were either highly abundant or expressed specifically in the olfactory organs. Furthermore, AjapOrco silencing resulted in a significant decrease in both the parasitism rate and the host-seeking time of A. japonicus, whereas dsRNA injection showed that IR8a and IR25a did not produce significant behavioral changes, suggesting that the oviposition behavior of A. japonicus is more reliant on OR-based pathways than IR-based pathways. Our previous GC-MS data derived twenty-nine compounds which were abundent from these host plants and host insects. We performed electrophysiological and oviposition assays on A. japonicus, and eight odorants were found to elicit a significant electroantennogram (EAG) response. Among these odorants, ß-Caryophyllene, Undecane, (E)-α-Farnesene (+)-Aromadendrene and Cis-3-Hexen-ol had strong attractant effects on oviposition, whereas 2-Ethyl-1-Hexan-ol, Ethyl Acetate and α-Caryophyllene had a strong repellant effects. Thus, these chemicals might influence oviposition guidance/repulsion behavior in A. japonicus. To further explore the target ORs that are tuned to the functional odorants, the nine candidate ORs described above were silenced by RNA interference, and the results showed that a large decrease in the EAG response of all the tested functional odorants in the AjapOrco-silencing group. In addition, the AjapOR35-silencing group showed a significant decrease in the EAG response to ß-Caryophyllene and (E)-α-Farnesene, indicating that AjapOR35 is tuned to these two oviposition attractants ß-Caryophyllene and (E)-α-Farnesene. Further binary-choice oviposition assays showed that the oviposition attractant effect of ß-Caryophyllene and (E)-α-Farnesene vanished after AjapOR35 was silenced, indicating that the emission of these attractants from host plants can guide A. japonicus to locate eggs for ovipositioning and indicated that AjapOR35 is correlated with the olfactory detection oviposition behavior of this species. This study provides a better understanding of the molecular basis and functional chemicals underlying the oviposition behavior of A. japonicus, and the results may help improve biocontrol approaches.
[Mh] Termos MeSH primário: Oviposição
Receptores Ionotrópicos de Glutamato/metabolismo
Receptores Odorantes/metabolismo
Olfato
Vespas/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Ionotropic Glutamate); 0 (Receptors, Odorant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


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[PMID]:28582411
[Au] Autor:Komisarczuk AZ; Grotmol S; Nilsen F
[Ad] Endereço:Sea Lice Research Centre, Department of Biology, University of Bergen, Bergen, Norway.
[Ti] Título:Ionotropic receptors signal host recognition in the salmon louse (Lepeophtheirus salmonis, Copepoda).
[So] Source:PLoS One;12(6):e0178812, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A remarkable feature of many parasites is a high degree of host specificity but the mechanisms behind are poorly understood. A major challenge for parasites is to identify and infect a suitable host. Many species show a high degree of host specificity, being able to survive only on one or a few related host species. To facilitate transmission, parasite's behavior and reproduction has been fine tuned to maximize the likelihood of infection of a suitable host. For some species chemical cues that trigger or attract the parasite in question have been identified but how metazoan parasites themselves receive these signals remains unknown. In the present study we show that ionotropic receptors (IRs) in the salmon louse are likely responsible for identification of a specific host. By using RNAi to knock down the expression level of different co-receptors, a significant change of infectivity and settlement of lice larvae was achieved on Atlantic salmon. More remarkably, knock down of the IRs changed the host specificity of the salmon louse and lice larvae settled at a significant rate on host that the wild type lice rejected within minutes. To our knowledge, this has never before been demonstrated for any metazoan parasite. Our results show that the parasites are able to identify the host quickly upon settlement, settle and initiate the parasitic life style if they are on the right host. This novel discovery opens up for utilizing the host recognition system for future parasite control.
[Mh] Termos MeSH primário: Copépodes/fisiologia
Especificidade de Hospedeiro
Larva/fisiologia
Percepção Olfatória/fisiologia
Receptores Ionotrópicos de Glutamato/metabolismo
Salmo salar/parasitologia
[Mh] Termos MeSH secundário: Animais
Ectoparasitoses/parasitologia
Feminino
Doenças dos Peixes/parasitologia
Expressão Gênica
Interações Hospedeiro-Parasita
Masculino
Perciformes/parasitologia
Interferência de RNA
RNA de Cadeia Dupla/genética
RNA de Cadeia Dupla/metabolismo
Receptores Ionotrópicos de Glutamato/antagonistas & inibidores
Receptores Ionotrópicos de Glutamato/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Double-Stranded); 0 (Receptors, Ionotropic Glutamate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178812


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[PMID]:28475556
[Au] Autor:Lecker I; Wang DS; Kaneshwaran K; Mazer CD; Orser BA
[Ad] Endereço:From the Departments of Physiology (I.L., D.-S.W., K.K., C.D.M., B.A.O.) and Anesthesia (C.D.M., B.A.O.), University of Toronto; Department of Anesthesia, Keenan Research Centre for Biomedical Science and the Li Ka Shing Knowledge Institute, St. Michael's Hospital (C.D.M.); and Department of Anesthesia, Sunnybrook Health Sciences Centre (B.A.O.), Toronto, Ontario, Canada.
[Ti] Título:High Concentrations of Tranexamic Acid Inhibit Ionotropic Glutamate Receptors.
[So] Source:Anesthesiology;127(1):89-97, 2017 Jul.
[Is] ISSN:1528-1175
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The antifibrinolytic drug tranexamic acid is structurally similar to the amino acid glycine and may cause seizures and myoclonus by acting as a competitive antagonist of glycine receptors. Glycine is an obligatory co-agonist of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Thus, it is plausible that tranexamic acid inhibits NMDA receptors by acting as a competitive antagonist at the glycine binding site. The aim of this study was to determine whether tranexamic acid inhibits NMDA receptors, as well as α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and kainate subtypes of ionotropic glutamate receptors. METHODS: Tranexamic acid modulation of NMDA, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, and kainate receptors was studied using whole cell voltage-clamp recordings of current from cultured mouse hippocampal neurons. RESULTS: Tranexamic acid rapidly and reversibly inhibited NMDA receptors (half maximal inhibitory concentration = 241 ± 45 mM, mean ± SD; 95% CI, 200 to 281; n = 5) and shifted the glycine concentration-response curve for NMDA-evoked current to the right. Tranexamic acid also inhibited α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (half maximal inhibitory concentration = 231 ± 91 mM; 95% CI, 148 to 314; n = 5 to 6) and kainate receptors (half maximal inhibitory concentration = 90 ± 24 mM; 95% CI, 68 to 112; n = 5). CONCLUSIONS: Tranexamic acid inhibits NMDA receptors likely by reducing the binding of the co-agonist glycine and also inhibits α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and kainate receptors. Receptor blockade occurs at high millimolar concentrations of tranexamic acid, similar to the concentrations that occur after topical application to peripheral tissues. Glutamate receptors in tissues including bone, heart, and nerves play various physiologic roles, and tranexamic acid inhibition of these receptors may contribute to adverse drug effects.
[Mh] Termos MeSH primário: Antifibrinolíticos/farmacologia
Receptores Ionotrópicos de Glutamato/efeitos dos fármacos
Ácido Tranexâmico/farmacologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Relação Dose-Resposta a Droga
Hipocampo/efeitos dos fármacos
Camundongos
Modelos Animais
Neurônios/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifibrinolytic Agents); 0 (Receptors, Ionotropic Glutamate); 6T84R30KC1 (Tranexamic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1097/ALN.0000000000001665


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[PMID]:28423037
[Au] Autor:Wei HS; Li KB; Zhang S; Cao YZ; Yin J
[Ad] Endereço:State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus.
[So] Source:PLoS One;12(4):e0174036, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Loxostege sticticalis Linnaeus is an economically important agricultural pest, and the larvae cause great damage to crops, especially in Northern China. However, effective and environmentally friendly chemical methods for controlling this pest have not been discovered to date. In the present study, we performed HiSeq2500 sequencing of transcriptomes of the male and female adult antennae, adult legs and third instar larvae, and we identified 54 candidate odorant receptors (ORs), including 1 odorant receptor coreceptor (Orco) and 5 pheromone receptors (PRs), 18 ionotropic receptors (IRs), 13 gustatory receptors (GRs), 34 odorant binding proteins (OBPs), including 1 general odorant binding protein (GOBP1) and 3 pheromone binding proteins (PBPs), 10 chemosensory proteins (CSPs) and 2 sensory neuron membrane proteins (SNMPs). The results of RNA-Seq and RT-qPCR analyses showed the expression levels of most genes in the antennae were higher than that in the legs and larvae. Furthermore, PR4, OR1-4, 7-11, 13-15, 23, 29-32, 34, 41, 43, 47/IR7d.2/GR5b, 45, 7/PBP2-3, GOBP1, OBP3, 8 showed female antennae-biased expression, while PR1/OBP2, 7/IR75d/CSP2 showed male antennae-biased expression. However, IR1, 7d.3, 68a/OBP11, 20-22, 28/CSP9 had larvae enriched expression, and OBP15, 17, 25, 29/CSP5 were mainly expressed in the legs. The results shown above indicated that these genes might play a key role in foraging, seeking mates and host recognition in the L. sticticalis. Our findings will provide the basic knowledge for further studies on the molecular mechanisms of the olfactory system of L. sticticalis and potential novel targets for pest control strategies.
[Mh] Termos MeSH primário: Antenas de Artrópodes/metabolismo
Proteínas de Insetos/genética
Lepidópteros/genética
Receptores Ionotrópicos de Glutamato/genética
Receptores Odorantes/genética
Receptores de Feromonas/genética
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Feminino
Perfilação da Expressão Gênica
Ontologia Genética
Proteínas de Insetos/metabolismo
Larva/genética
Lepidópteros/classificação
Masculino
Anotação de Sequência Molecular
Filogenia
Receptores Ionotrópicos de Glutamato/metabolismo
Receptores Odorantes/metabolismo
Receptores de Feromonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Receptors, Ionotropic Glutamate); 0 (Receptors, Odorant); 0 (Receptors, Pheromone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174036


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[PMID]:28399284
[Au] Autor:Chen Q; Man Y; Li J; Pei D; Wu W
[Ad] Endereço:College of Science, National University of Defense Technology, Changsha, Hunan, China.
[Ti] Título:Olfactory Ionotropic Receptors in Mosquito Aedes albopictus (Diptera: Culicidae).
[So] Source:J Med Entomol;54(5):1229-1235, 2017 Sep 01.
[Is] ISSN:1938-2928
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ionotropic glutamate receptors (iGluRs) are a conserved family of ligand-gated ion channels that primarily function to mediate neuronal communication at synapses. A variant subfamily of iGluRs, the ionotropic receptors (IRs), was recently identified in insects and proved with the function in odorant recognition. Ionotropic receptors participate in a distinct olfactory signaling pathway that is independent of olfactory receptors activity. In the present study, we identify 102 putative IR genes, dubbed as AalbIr genes, in mosquito Aedes albopictus (Skuse) by in silico comparative sequence analysis. Among AalbIr genes, 19 show expression in the female antenna by RT-PCR. These putative olfactory AalbIRs share four conservative hydrophobic domains of amino acids, similar to the transmembrane and ion channel pore regions found in conventional iGluRs. To determine the potential function of these olfactory AalbIRs in host-seeking, we compared their transcript expression levels in the antennae of blood-fed females with that of non-blood-fed females by quantitative real-time RT-PCR. Three AalbIr genes showed downregulation when the mosquito finished a bloodmeal. These results may help to improve our understanding of the IR-mediated olfactory signaling in mosquitoes.
[Mh] Termos MeSH primário: Aedes/metabolismo
Antenas de Artrópodes/metabolismo
Receptores Ionotrópicos de Glutamato/metabolismo
Receptores Odorantes/metabolismo
[Mh] Termos MeSH secundário: Aedes/genética
Sequência de Aminoácidos
Animais
Proteínas de Drosophila/genética
Comportamento Alimentar
Feminino
Filogenia
Reação em Cadeia da Polimerase em Tempo Real
Receptores Ionotrópicos de Glutamato/genética
Receptores Odorantes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (Receptors, Ionotropic Glutamate); 0 (Receptors, Odorant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1093/jme/tjx063


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[PMID]:28348379
[Au] Autor:Devor A; Andreassen OA; Wang Y; Mäki-Marttunen T; Smeland OB; Fan CC; Schork AJ; Holland D; Thompson WK; Witoelar A; Chen CH; Desikan RS; McEvoy LK; Djurovic S; Greengard P; Svenningsson P; Einevoll GT; Dale AM
[Ad] Endereço:Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA.
[Ti] Título:Genetic evidence for role of integration of fast and slow neurotransmission in schizophrenia.
[So] Source:Mol Psychiatry;22(6):792-801, 2017 Jun.
[Is] ISSN:1476-5578
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The most recent genome-wide association studies (GWAS) of schizophrenia (SCZ) identified hundreds of risk variants potentially implicated in the disease. Further, novel statistical methodology designed for polygenic architecture revealed more potential risk variants. This can provide a link between individual genetic factors and the mechanistic underpinnings of SCZ. Intriguingly, a large number of genes coding for ionotropic and metabotropic receptors for various neurotransmitters-glutamate, γ-aminobutyric acid (GABA), dopamine, serotonin, acetylcholine and opioids-and numerous ion channels were associated with SCZ. Here, we review these findings from the standpoint of classical neurobiological knowledge of neuronal synaptic transmission and regulation of electrical excitability. We show that a substantial proportion of the identified genes are involved in intracellular cascades known to integrate 'slow' (G-protein-coupled receptors) and 'fast' (ionotropic receptors) neurotransmission converging on the protein DARPP-32. Inspection of the Human Brain Transcriptome Project database confirms that that these genes are indeed expressed in the brain, with the expression profile following specific developmental trajectories, underscoring their relevance to brain organization and function. These findings extend the existing pathophysiology hypothesis by suggesting a unifying role of dysregulation in neuronal excitability and synaptic integration in SCZ. This emergent model supports the concept of SCZ as an 'associative' disorder-a breakdown in the communication across different slow and fast neurotransmitter systems through intracellular signaling pathways-and may unify a number of currently competing hypotheses of SCZ pathophysiology.
[Mh] Termos MeSH primário: Receptores Ionotrópicos de Glutamato/genética
Receptores de Glutamato Metabotrópico/genética
Esquizofrenia/genética
[Mh] Termos MeSH secundário: Encéfalo/metabolismo
Dopamina/metabolismo
Fosfoproteína 32 Regulada por cAMP e Dopamina/metabolismo
Predisposição Genética para Doença/genética
Estudo de Associação Genômica Ampla
Genótipo
Seres Humanos
Herança Multifatorial/genética
Polimorfismo de Nucleotídeo Único/genética
Receptores Ionotrópicos de Glutamato/metabolismo
Receptores de Glutamato Metabotrópico/metabolismo
Fatores de Risco
Transdução de Sinais/genética
Transmissão Sináptica/genética
Ácido gama-Aminobutírico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Dopamine and cAMP-Regulated Phosphoprotein 32); 0 (Receptors, Ionotropic Glutamate); 0 (Receptors, Metabotropic Glutamate); 56-12-2 (gamma-Aminobutyric Acid); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1038/mp.2017.33


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[PMID]:28334209
[Au] Autor:Zbinden M; Berthod C; Montagné N; Machon J; Léger N; Chertemps T; Rabet N; Shillito B; Ravaux J
[Ad] Endereço:Sorbonne Universités, Univ Paris 06, UMR CNRS MNHN 7208 Biologie des Organismes Aquatiques et Ecosystèmes (BOREA), Equipe Adaptation aux Milieux Extrêmes, Bât. A, 4e étage, 7 Quai St Bernard, 75005 Paris, France.
[Ti] Título:Comparative Study of Chemosensory Organs of Shrimp From Hydrothermal Vent and Coastal Environments.
[So] Source:Chem Senses;42(4):319-331, 2017 May 01.
[Is] ISSN:1464-3553
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The detection of chemical signals is involved in a variety of crustacean behaviors, such as social interactions, search and evaluation of food and navigation in the environment. At hydrothermal vents, endemic shrimp may use the chemical signature of vent fluids to locate active edifices, however little is known on their sensory perception in these remote deep-sea habitats. Here, we present the first comparative description of the sensilla on the antennules and antennae of 4 hydrothermal vent shrimp (Rimicaris exoculata, Mirocaris fortunata, Chorocaris chacei, and Alvinocaris markensis) and of a closely related coastal shrimp (Palaemon elegans). These observations revealed no specific adaptation regarding the size or number of aesthetascs (specialized unimodal olfactory sensilla) between hydrothermal and coastal species. We also identified partial sequences of the ionotropic receptor IR25a, a co-receptor putatively involved in olfaction, in 3 coastal and 4 hydrothermal shrimp species, and showed that it is mainly expressed in the lateral flagella of the antennules that bear the unimodal chemosensilla aesthetascs.
[Mh] Termos MeSH primário: Decápodes (Crustáceos)/fisiologia
Fontes Hidrotermais
Sensilas/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Animais
Receptores Ionotrópicos de Glutamato/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Ionotropic Glutamate)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/chemse/bjx007



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