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[PMID]: | 28931058 |
[Au] Autor: | Moura ARSS; Kretz CB; Ferreira IE; Nunes AMPB; de Moraes JC; Reis MG; McBride AJA; Wang X; Campos LC |
[Ad] Endereço: | Laboratório de Patologia e Biologia Molecular, Instituto Gonçalo Moniz, FIOCRUZ-BA, Salvador, Bahia, Brazil. |
[Ti] Título: | Molecular characterization of Neisseria meningitidis isolates recovered from 11-19-year-old meningococcal carriers in Salvador, Brazil. |
[So] Source: | PLoS One;12(9):e0185038, 2017. | [Is] ISSN: | 1932-6203 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Characterization of meningococci isolated from the pharynx is essential towards understanding the dynamics of meningococcal carriage and disease. Meningococcal isolates, collected from adolescents resident in Salvador, Brazil during 2014, were characterized by multilocus sequence typing, genotyping or whole-genome sequencing. Most were nongroupable (61.0%), followed by genogroups B (11.9%) and Y (8.5%). We identified 34 different sequence types (STs), eight were new STs, distributed among 14 clonal complexes (cc), cc1136 represented 20.3% of the nongroupable isolates. The porA and fetA genotypes included P1.18,25-37 (11.9%), P1.18-1,3 (10.2%); F5-5 (23.7%), F4-66 (16.9%) and F1-7 (13.6%). The porB class 3 protein and the fHbp subfamily A (variants 2 and 3) genotypes were found in 93.0 and 71.0% of the isolates, respectively. NHBA was present in all isolates, and while most lacked NadA (94.9%), we detected the hyperinvasive lineages B:P1.19,15:F5-1:ST-639 (cc32); C:P1.22,14-6:F3-9:ST-3780 (cc103) and W:P1.5,2:F1-1:ST-11 (cc11). This is the first report on the genetic diversity and vaccine antigen prevalence among N. meningitidis carriage isolates in the Northeast of Brazil. This study highlights the need for ongoing characterization of meningococcal isolates following the introduction of vaccines and for determining public health intervention strategies. |
[Mh] Termos MeSH primário: |
Neisseria meningitidis/genética Neisseria meningitidis/isolamento & purificação Filogenia
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[Mh] Termos MeSH secundário: |
Adesinas Bacterianas/genética Adolescente Antígenos de Bactérias/genética Proteínas da Membrana Bacteriana Externa/genética Proteínas de Bactérias/genética Brasil Proteínas de Transporte/genética Portador Sadio Criança Variação Genética Genótipo Seres Humanos Tipagem de Sequências Multilocus Porinas/genética Adulto Jovem
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Adhesins, Bacterial); 0 (Antigens, Bacterial); 0 (Bacterial Outer Membrane Proteins); 0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (FrpB protein, bacteria); 0 (NHBA protein, Neisseria meningitidis); 0 (NadA protein, Neisseria meningitidis); 0 (Porins); 0 (factor H-binding protein, Neisseria meningitidis); 0 (porin protein, Neisseria) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171017 |
[Lr] Data última revisão:
| 171017 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170921 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.pone.0185038 |
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