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  1 / 721 MEDLINE  
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[PMID]:28587519
[Au] Autor:Liu J; Zhang J; Guo L; Zhao W; Hu X; Wei X
[Ad] Endereço:a Guanghua School of Stomatology, Hospital of Stomatology , Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology , Guangzhou , PR China.
[Ti] Título:Inactivation of a putative efflux pump (LmrB) in Streptococcus mutans results in altered biofilm structure and increased exopolysaccharide synthesis: implications for biofilm resistance.
[So] Source:Biofouling;33(6):481-493, 2017 Jul.
[Is] ISSN:1029-2454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Efflux pumps are a mechanism associated with biofilm formation and resistance. There is limited information regarding efflux pumps in Streptococcus mutans, a major pathogen in dental caries. The aim of this study was to investigate potential roles of a putative efflux pump (LmrB) in S. mutans biofilm formation and susceptibility. Upon lmrB inactivation and antimicrobial exposure, the biofilm structure and expression of other efflux pumps were examined using confocal laser scanning microscopy (CLSM) and qRT-PCR. lmrB inactivation resulted in biofilm structural changes, increased EPS formation and EPS-related gene transcription (p < 0.05), but no improvement in susceptibility was observed. The expression of most efflux pump genes increased upon lmrB inactivation when exposed to antimicrobials (p < 0.05), suggesting a feedback mechanism that activated the transcription of other efflux pumps to compensate for the loss of lmrB. These observations imply that sole inactivation of lmrB is not an effective solution to control biofilms.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Biofilmes/efeitos dos fármacos
Farmacorresistência Bacteriana/genética
Expressão Gênica/efeitos dos fármacos
Genes Bacterianos
Polissacarídeos Bacterianos/biossíntese
Streptococcus mutans/efeitos dos fármacos
[Mh] Termos MeSH secundário: Biofilmes/crescimento & desenvolvimento
Cárie Dentária/microbiologia
Bombas de Íon/genética
Microscopia Confocal
Mutação
Streptococcus mutans/genética
Streptococcus mutans/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Ion Pumps); 0 (Polysaccharides, Bacterial); 0 (exopolysaccharide, Streptococcus)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1080/08927014.2017.1323206


  2 / 721 MEDLINE  
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[PMID]:28420099
[Au] Autor:Orlov SN; Klimanova EA; Tverskoi AM; Vladychenskaya EA; Smolyaninova LV; Lopina OD
[Ad] Endereço:Laboratory of Biological Membranes, Faculty of Biology, M.V. Lomonosov Moscow State University, 1/12 Leninskie Gory, Moscow 119234, Russia. sergeinorlov@yandex.ru.
[Ti] Título:Na⁺ ,K⁺ -Dependent and -Independent Signaling Triggered by Cardiotonic Steroids: Facts and Artifacts.
[So] Source:Molecules;22(4), 2017 Apr 14.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Na⁺,K⁺-ATPase is the only known receptor of cardiotonic steroids (CTS) whose interaction with catalytic α-subunits leads to inhibition of this enzyme. As predicted, CTS affect numerous cellular functions related to the maintenance of the transmembrane gradient of monovalent cations, such as electrical membrane potential, cell volume, transepithelial movement of salt and osmotically-obliged water, symport of Na⁺ with inorganic phosphate, glucose, amino acids, nucleotides, etc. During the last two decades, it was shown that side-by-side with these canonical Na⁺ /K⁺ -dependent cellular responses, long-term exposure to CTS affects transcription, translation, tight junction, cell adhesion and exhibits tissue-specific impact on cell survival and death. It was also shown that CTS trigger diverse signaling cascades via conformational transitions of the Na⁺,K⁺-ATPase α-subunit that, in turn, results in the activation of membrane-associated non-receptor tyrosine kinase Src, phosphatidylinositol 3-kinase and the inositol 1,4,5-triphosphate receptor. These findings allowed researchers to propose that endogenous CTS might be considered as a novel class of steroid hormones. We focus our review on the analysis of the relative impact Na⁺ ,K⁺ -mediated and -independent pathways in cellular responses evoked by CTS.
[Mh] Termos MeSH primário: Glicosídeos Cardíacos/farmacologia
Transdução de Sinais/efeitos dos fármacos
ATPase Trocadora de Sódio-Potássio/metabolismo
[Mh] Termos MeSH secundário: Animais
Glicosídeos Cardíacos/química
Adesão Celular/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Bombas de Íon/metabolismo
Extratos Vegetais/química
Extratos Vegetais/farmacologia
ATPase Trocadora de Sódio-Potássio/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cardiac Glycosides); 0 (Ion Pumps); 0 (Plant Extracts); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE


  3 / 721 MEDLINE  
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[PMID]:28220999
[Au] Autor:Søvik E; LaMora A; Seehra G; Barron AB; Duncan JG; Ben-Shahar Y
[Ad] Endereço:Department of Biology, Washington University, St. Louis, MO, USA.
[Ti] Título:Drosophila divalent metal ion transporter Malvolio is required in dopaminergic neurons for feeding decisions.
[So] Source:Genes Brain Behav;16(5):506-514, 2017 Jun.
[Is] ISSN:1601-183X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Members of the natural resistance-associated macrophage protein (NRAMP) family are evolutionarily conserved metal ion transporters that play an essential role in regulating intracellular divalent cation homeostasis in both prokaryotes and eukaryotes. Malvolio (Mvl), the sole NRAMP family member in insects, plays a role in food choice behaviors in Drosophila and other species. However, the specific physiological and cellular processes that require the action of Mvl for appropriate feeding decisions remain elusive. Here, we show that normal food choice requires Mvl function specifically in the dopaminergic system, and can be rescued by supplementing food with manganese. Collectively, our data indicate that the action of the Mvl transporter affects food choice behavior via the regulation of dopaminergic innervation of the mushroom bodies, a principle brain region associated with decision-making in insects. Our studies suggest that the homeostatic regulation of the intraneuronal levels of divalent cations plays an important role in the development and function of the dopaminergic system and associated behaviors.
[Mh] Termos MeSH primário: Comportamento de Escolha
Neurônios Dopaminérgicos/metabolismo
Proteínas de Drosophila/genética
Drosophila/metabolismo
Comportamento Alimentar
Bombas de Íon/genética
[Mh] Termos MeSH secundário: Animais
Neurônios Dopaminérgicos/efeitos dos fármacos
Neurônios Dopaminérgicos/fisiologia
Drosophila/genética
Drosophila/fisiologia
Proteínas de Drosophila/metabolismo
Bombas de Íon/metabolismo
Manganês/metabolismo
Manganês/farmacologia
Corpos Pedunculados/citologia
Corpos Pedunculados/metabolismo
Corpos Pedunculados/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (Ion Pumps); 0 (malvolio protein, Drosophila); 42Z2K6ZL8P (Manganese)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1111/gbb.12375


  4 / 721 MEDLINE  
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[PMID]:28175292
[Au] Autor:Zeng H; Liu J; Ling J
[Ad] Endereço:Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou, China.
[Ti] Título:Efflux inhibitor suppresses Streptococcus mutans virulence properties.
[So] Source:FEMS Microbiol Lett;364(7), 2017 Apr 01.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It is well established that efflux pumps play important roles in bacterial pathogenicity and efflux inhibitors (EIs) have been proved to be effective in suppressing bacterial virulence properties. However, little is known regarding the EI of Streptococcus mutans, a well-known caries-inducing bacterium. In this study, we identified the EI of S. mutans through ethidium bromide efflux assay and investigated how EI affected S. mutans virulence regarding the cariogenicity and stress response. Results indicated that reserpine, the identified EI, suppressed acid tolerance, mutacin production and transformation efficiency of S. mutans, and modified biofilm architecture and extracellular polysaccharide distribution. Suppressed glycosyltransferase activity was also noted after reserpine exposure. The data from quantitative real-time-PCR demonstrated that reserpine significantly altered the expression profile of quorum-sensing and virulence-associated genes. These findings suggest that reserpine represents a promising adjunct anticariogenic agent in that it suppresses virulence properties of S. mutans.
[Mh] Termos MeSH primário: Bombas de Íon/antagonistas & inibidores
Reserpina/farmacologia
Streptococcus mutans/efeitos dos fármacos
Streptococcus mutans/patogenicidade
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Bacteriocinas/metabolismo
Biofilmes/efeitos dos fármacos
Cárie Dentária/tratamento farmacológico
Cárie Dentária/microbiologia
Glicosiltransferases/antagonistas & inibidores
Testes de Sensibilidade Microbiana
Percepção de Quorum/efeitos dos fármacos
Reação em Cadeia da Polimerase em Tempo Real
Streptococcus mutans/genética
Virulência/efeitos dos fármacos
Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacteriocins); 0 (Ion Pumps); 0 (mutacin III, Streptococcus mutans); 8B1QWR724A (Reserpine); EC 2.4.- (Glycosyltransferases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1093/femsle/fnx033


  5 / 721 MEDLINE  
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[PMID]:27986916
[Au] Autor:Li Z; Wang X; Chen J; Gao J; Zhou X; Kuai B
[Ad] Endereço:State Key Laboratory of Genetic Engineering and Fudan Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
[Ti] Título:CCX1, a Putative Cation/Ca2+ Exchanger, Participates in Regulation of Reactive Oxygen Species Homeostasis and Leaf Senescence.
[So] Source:Plant Cell Physiol;57(12):2611-2619, 2016 Dec.
[Is] ISSN:1471-9053
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The major developmental significance of leaf senescence is the massive recycling of nutrients from senescing leaves to nascent organs, including seeds, to meet the requirement of their rapid development, so-called nutrient remobilization. The efficiency of nutrient remobilization is associated with the activity of diverse transporters. A large number of transporters are up-regulated during leaf senescence in Arabidopsis, many of which participate in regulating leaf senescence via different signaling pathways. Here, we report that a member of the cation/Ca exchanger family, CCX1, is highly induced during leaf senescence in Arabidopsis. Although single mutation of CCX1 did not change the senescence phenotype, double mutation of CCX1 and CCX4 resulted in a subtle but significant stay-green phenotype during natural and dark-induced leaf senescence, suggesting that some members of the cation/Ca exchanger family act redundantly in mediating leaf senescence. Consistently, overexpression of CCX1 accelerated leaf senescence. Moreover, the ccx1ccx4 double mutant was more tolerant to H O , whereas CCX1-overexpressing lines showed an elevated response to H O treatment, presumably due to an overaccumulation of reactive oxygen species (ROS), indicating that CCX1 may promote leaf senescence via modulating ROS homeostasis. Notably, both ccx1-1 and ccx1ccx4 were sensitive to Ca deprivation, implying that CCX1 may also be involved in modulating Ca signaling and consequently affecting the initiation of leaf senescence.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/genética
Cálcio/metabolismo
Cátions/metabolismo
Regulação da Expressão Gênica de Plantas
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Antiporters
Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Senescência Celular
Escuridão
Expressão Gênica
Homeostase
Peróxido de Hidrogênio/metabolismo
Bombas de Íon/genética
Bombas de Íon/metabolismo
Mutação
Fenótipo
Folhas de Planta/genética
Folhas de Planta/metabolismo
Plantas Geneticamente Modificadas
Sementes/genética
Sementes/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiporters); 0 (Arabidopsis Proteins); 0 (CAX7 calcium exchanger 7, Arabidopsis thaliana); 0 (Cations); 0 (Ion Pumps); 0 (Reactive Oxygen Species); BBX060AN9V (Hydrogen Peroxide); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE


  6 / 721 MEDLINE  
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[PMID]:27525850
[Au] Autor:Yang P; Hua C; Zhou F; Zhang BJ; Cai XN; Chen QZ; Wang RL
[Ad] Endereço:School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing, China.
[Ti] Título:Molecular evolutionary analysis of the high-affinity K+ transporter gene family in angiosperms.
[So] Source:Genet Mol Res;15(3), 2016 Jul 15.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The high-affinity K(+) transporter (HKT) family comprises a group of multifunctional cation transporters widely distributed in organisms ranging from Bacteria to Eukarya. In angiosperms, the HKT family consists primarily of nine types, whose evolutionary relationships are not fully understood. The available sequences from 31 plant species were used to perform a comprehensive evolutionary analysis, including an examination of selection pressure and estimating phylogenetic tree and gene duplication events. Our results show that a gene duplication in the HKT1;5/HKT1;4 cluster might have led to the divergence of the HKT1;5 and HKT1;4 subfamilies. Additionally, maximum likelihood analysis revealed that the HKT family has undergone a strong purifying selection. An analysis of the amino acids provided strong statistical evidence for a functional divergence between subfamilies 1 and 2. Our study was the first to provide evidence of this functional divergence between these two subfamilies. Analysis of co-evolution in HKT identified 25 co-evolved groups. These findings expanded our understanding of the evolutionary mechanisms driving functional diversification of HKT proteins.
[Mh] Termos MeSH primário: Evolução Molecular
Bombas de Íon/genética
Magnoliopsida/genética
Proteínas de Plantas/genética
Potássio/metabolismo
[Mh] Termos MeSH secundário: Duplicação Gênica
Bombas de Íon/metabolismo
Magnoliopsida/classificação
Filogenia
Proteínas de Plantas/metabolismo
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ion Pumps); 0 (Plant Proteins); RWP5GA015D (Potassium)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15038781


  7 / 721 MEDLINE  
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[PMID]:27105427
[Au] Autor:Atia J; McCloskey C; Shmygol AS; Rand DA; van den Berg HA; Blanks AM
[Ad] Endereço:Division of Reproductive Health, Warwick Medical School, University of Warwick, Coventry, United Kingdom.
[Ti] Título:Reconstruction of Cell Surface Densities of Ion Pumps, Exchangers, and Channels from mRNA Expression, Conductance Kinetics, Whole-Cell Calcium, and Current-Clamp Voltage Recordings, with an Application to Human Uterine Smooth Muscle Cells.
[So] Source:PLoS Comput Biol;12(4):e1004828, 2016 Apr.
[Is] ISSN:1553-7358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Uterine smooth muscle cells remain quiescent throughout most of gestation, only generating spontaneous action potentials immediately prior to, and during, labor. This study presents a method that combines transcriptomics with biophysical recordings to characterise the conductance repertoire of these cells, the 'conductance repertoire' being the total complement of ion channels and transporters expressed by an electrically active cell. Transcriptomic analysis provides a set of potential electrogenic entities, of which the conductance repertoire is a subset. Each entity within the conductance repertoire was modeled independently and its gating parameter values were fixed using the available biophysical data. The only remaining free parameters were the surface densities for each entity. We characterise the space of combinations of surface densities (density vectors) consistent with experimentally observed membrane potential and calcium waveforms. This yields insights on the functional redundancy of the system as well as its behavioral versatility. Our approach couples high-throughput transcriptomic data with physiological behaviors in health and disease, and provides a formal method to link genotype to phenotype in excitable systems. We accurately predict current densities and chart functional redundancy. For example, we find that to evoke the observed voltage waveform, the BK channel is functionally redundant whereas hERG is essential. Furthermore, our analysis suggests that activation of calcium-activated chloride conductances by intracellular calcium release is the key factor underlying spontaneous depolarisations.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Modelos Biológicos
Miócitos de Músculo Liso/metabolismo
Miométrio/metabolismo
[Mh] Termos MeSH secundário: Potenciais de Ação
Fenômenos Biofísicos
Membrana Celular/metabolismo
Biologia Computacional
Simulação por Computador
Feminino
Perfilação da Expressão Gênica
Seres Humanos
Ativação do Canal Iônico
Canais Iônicos/genética
Canais Iônicos/metabolismo
Bombas de Íon/genética
Bombas de Íon/metabolismo
Transporte de Íons
Cinética
Potenciais da Membrana
Miométrio/citologia
Técnicas de Patch-Clamp
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ion Channels); 0 (Ion Pumps); 0 (RNA, Messenger); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160423
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pcbi.1004828


  8 / 721 MEDLINE  
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[PMID]:27055221
[Au] Autor:Nielsen E; Ivarsen A; Erlandsen M; Hjortdal J
[Ad] Endereço:*Department of Ophthalmology, Aarhus University Hospital, Aarhus, Denmark; and †Section of Biostatistics, Department of Public Health, Aarhus University Hospital, Aarhus, Denmark.
[Ti] Título:Evaluation of Endothelial Pump Function in Fuchs Endothelial Dystrophy Before and After Endothelial Keratoplasty.
[So] Source:Cornea;35(6):878-83, 2016 Jun.
[Is] ISSN:1536-4798
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate the endothelial pump function in vivo after Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: In a prospective controlled trial, a group of 17 patients with Fuchs endothelial corneal dystrophy (FECD) eligible for DSAEK surgery and a group of 15 patients with cataract but with normal corneas eligible for cataract surgery (controls) were formed. A low oxygen-permeable contact lens was used to induce corneal edema. Changes in central corneal thickness were monitored as an indirect measure of endothelial cell pump function. Experiments were performed before surgery and repeated 12 months after surgery. RESULTS: Comparing the FECD and control groups before surgery, there was 24.8% (13.5-36.1) more edema in the FECD group after 2 hours (P < 0.001) and 19.9% (8.6-31.3) more edema in the FECD group after 3 hours (P < 0.001). In the FECD group, there was 15% (3.1-26.9) less edema after DSAEK than before surgery (P = 0.015) after 3 hours. Comparing the DSAEK-treated eyes with the control eyes 12 months after surgery; there was 12.8% (3.5-22.1) more edema in the DSAEK group after 2 hours (P = 0.007), but after 3 hours, the percentages of edema were similar (P = 0.11). CONCLUSIONS: Twelve months after DSAEK surgery, the grafted endothelium cleared the induced edema as fast as the control group, indicating a viable and near-normal endothelial pump function. However, significant differences in the deswelling patterns were detected, which may be caused by the added corneal stroma after DSAEK.
[Mh] Termos MeSH primário: Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior
Epitélio Posterior/fisiologia
Distrofia Endotelial de Fuchs/fisiopatologia
Distrofia Endotelial de Fuchs/cirurgia
Bombas de Íon/fisiologia
[Mh] Termos MeSH secundário: Idoso
Catarata
Extração de Catarata
Edema da Córnea/fisiopatologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Estudos Prospectivos
ATPase Trocadora de Sódio-Potássio/metabolismo
[Pt] Tipo de publicação:CONTROLLED CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ion Pumps); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160408
[St] Status:MEDLINE
[do] DOI:10.1097/ICO.0000000000000821


  9 / 721 MEDLINE  
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[PMID]:26965126
[Au] Autor:Pawela C; DeYoe E; Pashaie R
[Ad] Endereço:Biophysics Department, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA.
[Ti] Título:Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex.
[So] Source:Methods Mol Biol;1408:227-41, 2016.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Optogenetics is rapidly gaining acceptance as a preferred method to study specific neuronal cell types using light. Optogenetic neuromodulation requires the introduction of a cell-specific viral vector encoding for a light activating ion channel or ion pump and the utilization of a system to deliver light stimulation to brain. Here, we describe a two-part methodology starting with a procedure to inject an optogenetic AAV virus into rat cortex followed by a second procedure to surgically implant an optical cannula for light delivery to the deeper cortical layers.
[Mh] Termos MeSH primário: Córtex Cerebelar/fisiologia
Vetores Genéticos/genética
Optogenética/métodos
Estimulação Luminosa/métodos
Vírus/genética
[Mh] Termos MeSH secundário: Animais
Córtex Cerebelar/efeitos da radiação
Córtex Cerebelar/cirurgia
Desenho de Equipamento
Técnicas de Transferência de Genes/instrumentação
Vetores Genéticos/administração & dosagem
Canais Iônicos/genética
Bombas de Íon/genética
Luz
Optogenética/instrumentação
Estimulação Luminosa/instrumentação
Ratos
Rodopsina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Ion Channels); 0 (Ion Pumps); 9009-81-8 (Rhodopsin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-3512-3_15


  10 / 721 MEDLINE  
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[PMID]:26929409
[Au] Autor:Inoue K; Nomura Y; Kandori H
[Ad] Endereço:From the Department of Frontier Materials and OptoBioTechnology Research Center, Nagoya Institute of Technology, Showa-ku, Nagoya 466-8555, Japan and PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
[Ti] Título:Asymmetric Functional Conversion of Eubacterial Light-driven Ion Pumps.
[So] Source:J Biol Chem;291(19):9883-93, 2016 May 06.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In addition to the well-known light-driven outward proton pumps, novel ion-pumping rhodopsins functioning as outward Na(+) and inward Cl(-) pumps have been recently found in eubacteria. They convert light energy into transmembrane electrochemical potential difference, similar to the prototypical archaeal H(+) pump bacteriorhodopsin (BR) and Cl(-) pump halorhodopsin (HR). The H(+), Na(+), and Cl(-) pumps possess the conserved respective DTE, NDQ, and NTQ motifs in the helix C, which likely serve as their functional determinants. To verify this hypothesis, we attempted functional interconversion between selected pumps from each category by mutagenesis. Introduction of the proton-pumping motif resulted in successful Na(+) → H(+) functional conversion. Introduction of the respective characteristic motifs with several additional mutations leads to successful Na(+) → Cl(-) and Cl(-) → H(+) functional conversions, whereas remaining conversions (H(+) → Na(+), H(+) → Cl(-), Cl(-) → Na(+)) were unsuccessful when mutagenesis of 4-6 residues was used. Phylogenetic analysis suggests that a H(+) pump is the common ancestor of all of these rhodopsins, from which Cl(-) pumps emerged followed by Na(+) pumps. We propose that successful functional conversions of these ion pumps are achieved exclusively when mutagenesis reverses the evolutionary amino acid sequence changes. Dependence of the observed functional conversions on the direction of evolution strongly suggests that the essential structural mechanism of an ancestral function is retained even after the gain of a new function during natural evolution, which can be evoked by a few mutations. By contrast, the gain of a new function needs accumulation of multiple mutations, which may not be easily reproduced by limited mutagenesis in vitro.
[Mh] Termos MeSH primário: Bacteriorodopsinas/metabolismo
Eubacterium/metabolismo
Halorrodopsinas/metabolismo
Bombas de Íon/metabolismo
Transporte de Íons/efeitos da radiação
Luz
[Mh] Termos MeSH secundário: Fenômenos Fisiológicos Bacterianos
Bacteriorodopsinas/genética
Bacteriorodopsinas/efeitos da radiação
Cloretos/metabolismo
Eubacterium/efeitos da radiação
Halorrodopsinas/genética
Halorrodopsinas/efeitos da radiação
Bombas de Íon/química
Bombas de Íon/efeitos da radiação
Mutação/genética
Filogenia
Sódio/metabolismo
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chlorides); 0 (Halorhodopsins); 0 (Ion Pumps); 53026-44-1 (Bacteriorhodopsins); 9NEZ333N27 (Sodium)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170506
[Lr] Data última revisão:
170506
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.716498



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