Base de dados : MEDLINE
Pesquisa : D12.776.157.530.450.074.500.299 [Categoria DeCS]
Referências encontradas : 117 [refinar]
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  1 / 117 MEDLINE  
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[PMID]:28510138
[Au] Autor:Rosenkranz AA; Slastnikova TA; Khramtsov YV; Karyagina TS; Georgiev GP; Sobolev AS
[Ad] Endereço:Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia.
[Ti] Título:Antitumor efficacy of Auger electron emitter In delivered by modular nanotransporter into the nuclei of cells with folate receptor overexpression.
[So] Source:Dokl Biochem Biophys;473(1):85-87, 2017 Mar.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:A new modular nanotransporter (MNT) for the delivery of anticancer agents into the nuclei of cells with folate receptor overexpression was created. An effective method for acceding labeling of this MNT with Auger electron emitter In has been developed. A significant therapeutic effect was observed after a single intratumoral injection of the new In-labeled MNT to mice grafted with human cervical carcinoma characterized by folate receptor overexpression.
[Mh] Termos MeSH primário: Núcleo Celular/metabolismo
Elétrons
Transportadores de Ácido Fólico/metabolismo
Regulação Neoplásica da Expressão Gênica
Radioisótopos de Índio/metabolismo
Radioisótopos de Índio/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Células HeLa
Seres Humanos
Radioisótopos de Índio/administração & dosagem
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Folic Acid Transporters); 0 (Indium Radioisotopes)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.1134/S1607672917020016


  2 / 117 MEDLINE  
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[PMID]:28231500
[Au] Autor:Thapa RK; Kim JH; Jeong JH; Shin BS; Choi HG; Yong CS; Kim JO
[Ad] Endereço:College of Pharmacy, Yeungnam University, 280 Daehak-Ro, Gyeongsan, 712-749, Republic of Korea.
[Ti] Título:Silver nanoparticle-embedded graphene oxide-methotrexate for targeted cancer treatment.
[So] Source:Colloids Surf B Biointerfaces;153:95-103, 2017 May 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Combination therapies are widely investigated cancer treatment modalities. Carbon based systems such as graphene oxide (GO), plasmonic nanoparticles such as silver nanoparticles (AgNPs), and the folate analog, methotrexate (MTX), have been separately studied for their potential anticancer effects. In this study, we combined these systems to develop AgNPs-embedded GO with conjugated MTX (MTX-GO/AgNPs) and studied their folate receptor-targeted anticancer effects. Results revealed successful formation of AgNPs on GO along with MTX conjugation as suggested by UV/visible, TEM, AFM, FTIR, and XRD analysis. Folate receptor-positive MCF-7 cells were more prone to cytotoxic effects of MTX-GO/AgNPs compared to folate receptor-negative HepG2 cells. Folic acid analog MTX interacts with folate receptors expressed in MCF-7 cells, improving cellular uptake and subsequent anticancer effects of the system. Importantly, AgNPs enhanced the total ROS production within the treated cells leading to improve cellular apoptosis, as evidenced by western blot. Moreover, near infrared (NIR)-induced photothermal effects of GO improved the anticancer activity of the system. Therefore, the combinational therapy system MTX-GO/AgNPs can be potentially applied for effective folate receptor-targeted treatment of cancers.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Grafite/farmacologia
Nanopartículas Metálicas/química
Metotrexato/farmacologia
Óxidos/farmacologia
Prata/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Apoptose/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Transportadores de Ácido Fólico/antagonistas & inibidores
Transportadores de Ácido Fólico/metabolismo
Grafite/química
Células Hep G2
Seres Humanos
Células MCF-7
Metotrexato/química
Estrutura Molecular
Óxidos/química
Tamanho da Partícula
Espécies Reativas de Oxigênio/metabolismo
Prata/química
Relação Estrutura-Atividade
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Folic Acid Transporters); 0 (Oxides); 0 (Reactive Oxygen Species); 3M4G523W1G (Silver); 7782-42-5 (Graphite); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE


  3 / 117 MEDLINE  
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[PMID]:28103309
[Au] Autor:Castaño E; Caviedes L; Hirsch S; Llanos M; Iñiguez G; Ronco AM
[Ad] Endereço:Laboratory of Nutrition and Metabolic Regulation, Human Nutrition Unit, Institute of Nutrition and Food Technology, Dr. Fernando Monckeberg Barros (INTA), University of Chile, Santiago, Chile.
[Ti] Título:Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels.
[So] Source:PLoS One;12(1):e0170389, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Folate deficiency during pregnancy has been related to low birth weight, preterm (PT) birth and other health risks in the offspring; however, it is unknown whether prematurity is related to low folate transport through the placenta due to altered expression of specific folate transporters. We determined placental expression (mRNA and protein concentrations by RT-qPCR and WB respectively) of specific folate transporters: RFC, PCFT/HCP1 and FOLR1 in chorionic (fetal) and basal (maternal) plates of placentas of PT pregnancies (PT, 32-36 weeks, n = 51). Term placentas were used as controls (T, 37-41 weeks, n = 47). Folates and vitamin B12 levels were measured by electrochemiluminescence in umbilical cord blood of newborns. FOLR1 mRNA expression was lower and protein concentration higher in PT placentas (both plates) relative to the control group (p <0.05). In addition, gestational age was positively correlated with mRNA expression (Rho = 0.7), and negatively with protein concentration (Rho = -0.7 for chorionic and -0.43 for basal plate). PCFT/HCP1 mRNA was lower in PT placentas, without changes in protein levels. RFC did not differ in PT placentas compared to controls. PT newborns presented higher cord blood folate level (p = 0.049) along with lower vitamin B12 concentration compared to controls (p = 0.037).In conclusion, placental FOLR1 mRNA was positively associated with gestational age. Conversely, FOLR1 protein concentrations along with folate/vitamin B12 ratio in cord blood were negatively associated with gestational age. Placental FOLR1 is likely the main placental folate transporter to the fetus in newborns.
[Mh] Termos MeSH primário: Sangue Fetal/metabolismo
Transportadores de Ácido Fólico/metabolismo
Ácido Fólico/sangue
Placenta/metabolismo
Vitamina B 12/sangue
[Mh] Termos MeSH secundário: Adulto
Feminino
Receptor 1 de Folato/genética
Receptor 1 de Folato/metabolismo
Transportadores de Ácido Fólico/genética
Seres Humanos
Recém-Nascido
Recém-Nascido Prematuro
Gravidez
Nascimento Prematuro/sangue
Nascimento Prematuro/genética
Nascimento Prematuro/metabolismo
Transportador de Folato Acoplado a Próton/genética
Transportador de Folato Acoplado a Próton/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Proteína Carregadora de Folato Reduzido/genética
Proteína Carregadora de Folato Reduzido/metabolismo
Nascimento a Termo/sangue
Nascimento a Termo/genética
Nascimento a Termo/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FOLR1 protein, human); 0 (Folate Receptor 1); 0 (Folic Acid Transporters); 0 (Proton-Coupled Folate Transporter); 0 (RNA, Messenger); 0 (Reduced Folate Carrier Protein); 0 (SLC19A1 protein, human); 0 (SLC46A1 protein, human); 935E97BOY8 (Folic Acid); P6YC3EG204 (Vitamin B 12)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170389


  4 / 117 MEDLINE  
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[PMID]:28095689
[Au] Autor:Chen J; Klem S; Jones AK; Orr B; Banaszak Holl MM
[Ad] Endereço:Department of Chemistry, ‡Department of Biomedical Engineering, §Department of Physics, and ∥Program in Macromolecular Science and Engineering, University of Michigan , Ann Arbor, Michigan 48109, United States.
[Ti] Título:Folate-Binding Protein Self-Aggregation Drives Agglomeration of Folic Acid Targeted Iron Oxide Nanoparticles.
[So] Source:Bioconjug Chem;28(1):81-87, 2017 Jan 18.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Folate-conjugated nanomaterials have been widely investigated for drug and imaging-agent delivery. In this work, two folic acid (FA) conjugated iron oxide particles (IOP), a ∼40 nm diameter FA-IOP and a ∼450 nm diameter FA-IOP(FA-SeraMag), were synthesized. Both particles aggregated in the presence of serum folate-binding protein (FBP) at physiological concentration and buffer conditions. Mixing 0.01% w/w FA-conjugated iron oxide particles with FBP-induced agglomeration generated an average hydrodynamic particle diameter of 3800 ± 1100 nm for ∼40 nm FA-IOP and 4030 ± 1100 nm for FA-SeraMag as measured by dynamic light scattering (DLS). The presence of excess human serum albumin (HSA) (600 µM) did not prevent agglomeration of the ∼40 nm FA-IOP; however, it did inhibit agglomeration of FA-SeraMag. Atomic force microscopy measurement provided additional insight into particle morphology with the detection of individual particles in the agglomerate. This behavior is an example of a triggered cascade. A protein structural change is induced by FA binding, and the structural change favors aggregation of the ∼4 nm diameter FBPs on the particle surface; this further triggers the agglomeration of both the ∼40 and ∼450 nm diameter IOPs.
[Mh] Termos MeSH primário: Compostos Férricos/metabolismo
Transportadores de Ácido Fólico/metabolismo
Ácido Fólico/metabolismo
Nanopartículas Metálicas
[Mh] Termos MeSH secundário: Seres Humanos
Microscopia de Força Atômica
Albumina Sérica/metabolismo
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ferric Compounds); 0 (Folic Acid Transporters); 0 (Serum Albumin); 1K09F3G675 (ferric oxide); 935E97BOY8 (Folic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.6b00526


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[PMID]:28202841
[Au] Autor:Tsuji A; Noguchi R; Nakamura T; Shibata K
[Ad] Endereço:Department of Nutrition, School of Human Cultures, The University of Shiga Prefecture.
[Ti] Título:Folic Acid Deficiency Does Not Adversely Affect Oocyte Meiosis in Mice.
[So] Source:J Nutr Sci Vitaminol (Tokyo);62(6):375-379, 2016.
[Is] ISSN:1881-7742
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Spindle defect and chromosome misalignment occuring in oocyte meiosis induce nondisjunction. Nondisjunction causes Down syndrome, also known as trisomy 21. Folic acid (FA) is an essential nutrient composition for fetal growth and development. It has been reported that FA nutritional status is associated with the risk of Down syndrome. However, to our knowledge, little is known about the effect of FA deficiency on abnormal oocytes (spindle defects, chromosome misalignments and immature oocyte) in vivo. In the present study, we investigate the effects of FA deficiency on oocyte meiosis in female mice. In order to induce FA deficiency in mice, female Crl:CD1 mice were fed a FA-free diet for 58 d. The diet also contained an antibiotic which has functions on limiting FA formation by intestinal microorganisms. The level of FA deficiency was determined by measuring the concentration of FA in the liver, hemocyte, uterus, ovary, and urine. FA concentrations in these samples from the FA-deficient group were 50-90% lower. Despite this, the frequency of abnormal oocytes was no different between the FA-deficient and control groups (20.0% vs 14.6%). According to the past research, FA transporter was strongly expressed in oocytes. Hence, it is possible that FA-free diets may not affect the concentration of oocyte FA in mice. To sum up these data, our study concluded that FA deficiency did not adversely affect oocyte meiosis.
[Mh] Termos MeSH primário: Deficiência de Ácido Fólico/patologia
Meiose
Oócitos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Dieta
Feminino
Ácido Fólico/farmacologia
Ácido Fólico/urina
Deficiência de Ácido Fólico/tratamento farmacológico
Transportadores de Ácido Fólico/genética
Transportadores de Ácido Fólico/metabolismo
Hemócitos/metabolismo
Fígado/metabolismo
Camundongos
Oócitos/citologia
Ovário/metabolismo
Útero/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Folic Acid Transporters); 935E97BOY8 (Folic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.3177/jnsv.62.375


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[PMID]:27886088
[Au] Autor:Adamo G; Grimaldi N; Campora S; Bulone D; Bondì ML; Al-Sheikhly M; Sabatino MA; Dispenza C; Ghersi G
[Ad] Endereço:Dipartimento di Scienze e Tecnologie Molecolari e Biomolecolari, Università degli Studi di Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy. giorgia.adamo@unipa.it.
[Ti] Título:Multi-Functional Nanogels for Tumor Targeting and Redox-Sensitive Drug and siRNA Delivery.
[So] Source:Molecules;21(11), 2016 Nov 23.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:(1) Background: A new family of nanosystems able to discern between normal and tumor cells and to release a therapeutic agent in controlled way were synthetized by e-beam irradiation. This technique permits to obtain biocompatible, sterile, carboxyl-functionalized polyvinylpyrrolidone (PVP-co-acrylic acid) nanogels (NGs); (2) Methods: Here, we performed a targeting strategy based on the recognition of over-expressed proteins on tumor cells, like the folate receptor. The selective targeting was demonstrated by co-culture studies and flow cytometry analysis, using folate conjugated NGs. Moreover, nanoparticles were conjugated to a chemotherapeutic drug or to a pro-apoptotic siRNA through a glutathione sensitive spacer, in order to obtain a controlled release mechanism, specific for cancer cells. The drug efficiency was tested on tumor and healthy cells by flow cytometric analysis, confocal and epifluorescence microscopy and cytotoxicity assay; the siRNA effect was investigated by RNAi experiment; (3) Results: The data obtained showed that the use of NGs permits a faster cargo release in cancer cells, in response to high cytosolic glutathione level, also improving their efficacy; (4) Conclusion: The possibility of releasing biological molecules in a controlled way and to recognize a specific tumor target allows overcoming the typical limits of the classic cancer therapy.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Doxorrubicina/farmacologia
Neoplasias/metabolismo
Polietilenoglicóis/química
Polietilenoimina/química
RNA Interferente Pequeno/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Linhagem Celular Tumoral
Ácido Fólico/química
Ácido Fólico/metabolismo
Transportadores de Ácido Fólico/antagonistas & inibidores
Células HeLa
Seres Humanos
Camundongos
Células NIH 3T3
Nanopartículas/química
Neoplasias/tratamento farmacológico
Oxirredução/efeitos dos fármacos
Tamanho da Partícula
Polietilenoglicóis/farmacologia
Polietilenoimina/farmacologia
Povidona/química
Povidona/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Folic Acid Transporters); 0 (NanoGel); 0 (RNA, Small Interfering); 30IQX730WE (Polyethylene Glycols); 80168379AG (Doxorubicin); 9002-98-6 (Polyethyleneimine); 935E97BOY8 (Folic Acid); FZ989GH94E (Povidone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


  7 / 117 MEDLINE  
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[PMID]:27703008
[Au] Autor:Dewar S; Sienkiewicz N; Ong HB; Wall RJ; Horn D; Fairlamb AH
[Ad] Endereço:From the Division of Biological Chemistry and Drug Discovery, Wellcome Trust Building, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom.
[Ti] Título:The Role of Folate Transport in Antifolate Drug Action in Trypanosoma brucei.
[So] Source:J Biol Chem;291(47):24768-24778, 2016 Nov 18.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to identify and characterize mechanisms of resistance to antifolate drugs in African trypanosomes. Genome-wide RNAi library screens were undertaken in bloodstream form Trypanosoma brucei exposed to the antifolates methotrexate and raltitrexed. In conjunction with drug susceptibility and folate transport studies, RNAi knockdown was used to validate the functions of the putative folate transporters. The transport kinetics of folate and methotrexate were further characterized in whole cells. RNA interference target sequencing experiments identified a tandem array of genes encoding a folate transporter family, TbFT1-3, as major contributors to antifolate drug uptake. RNAi knockdown of TbFT1-3 substantially reduced folate transport into trypanosomes and reduced the parasite's susceptibly to the classical antifolates methotrexate and raltitrexed. In contrast, knockdown of TbFT1-3 increased susceptibly to the non-classical antifolates pyrimethamine and nolatrexed. Both folate and methotrexate transport were inhibited by classical antifolates but not by non-classical antifolates or biopterin. Thus, TbFT1-3 mediates the uptake of folate and classical antifolates in trypanosomes, and TbFT1-3 loss-of-function is a mechanism of antifolate drug resistance.
[Mh] Termos MeSH primário: Transportadores de Ácido Fólico/metabolismo
Ácido Fólico/metabolismo
Metotrexato/farmacocinética
Proteínas de Protozoários/metabolismo
Trypanosoma brucei brucei/metabolismo
[Mh] Termos MeSH secundário: Transportadores de Ácido Fólico/genética
Estudo de Associação Genômica Ampla
Metotrexato/farmacologia
Proteínas de Protozoários/genética
Trypanosoma brucei brucei/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Folic Acid Transporters); 0 (Protozoan Proteins); 935E97BOY8 (Folic Acid); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE


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[PMID]:27682214
[Au] Autor:Luo CQ; Jang Y; Xing L; Cui PF; Qiao JB; Lee AY; Kim HJ; Cho MH; Jiang HL
[Ad] Endereço:State Key Laboratory of Natural Medicines, Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
[Ti] Título:Aerosol delivery of folate-decorated hyperbranched polyspermine complexes to suppress lung tumorigenesis via Akt signaling pathway.
[So] Source:Int J Pharm;513(1-2):591-601, 2016 Nov 20.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lung cancer has been a leading cause of cancer mortality worldwide and aerosol-mediated gene therapy endows numerous advantages compared to other traditional modalities. Here, we reported a folic acid (FA)-modified hyperbranched polyspermine (HPSPE) with prominent biocompatibility for lung cancer cell targeted gene therapy. FA was decorated to the HPSPE via an amidation reaction and the physicochemical properties of nanoplexes formed with DNA were characterized. Gel electrophoresis study elucidated that the designed polymer was capable to condense DNA and protect it from degradation by DNase I. Cell viability and transfection efficiency assay in vitro and in vivo indicated its increased transfection performance with lower toxicity. Furthermore, reduced tumor numbers and down-regulation of Akt1 protein after aerosol treatment containing FA-HPSPE/shAkt1 complexes proved its therapeutic potential for lung cancer suppression. Results obtained in this study suggested that FA-HPSPE with highly biocompatibility and targeting capability while forming complexes with shAkt1 and administrated through noninvasive aerosol could be prospective for inhibiting lung tumorigenesis.
[Mh] Termos MeSH primário: Antineoplásicos/administração & dosagem
DNA/administração & dosagem
Ácido Fólico/administração & dosagem
Proteínas Proto-Oncogênicas c-akt/metabolismo
Espermina/análogos & derivados
Espermina/administração & dosagem
[Mh] Termos MeSH secundário: Aerossóis
Animais
Antineoplásicos/química
Antineoplásicos/farmacologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
DNA/química
DNA/farmacologia
Ácido Fólico/química
Ácido Fólico/farmacologia
Transportadores de Ácido Fólico/genética
Técnicas de Transferência de Genes
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Seres Humanos
Neoplasias Pulmonares/metabolismo
Neoplasias Pulmonares/terapia
Masculino
Camundongos Endogâmicos C57BL
Espermina/química
Espermina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Antineoplastic Agents); 0 (Folic Acid Transporters); 147336-22-9 (Green Fluorescent Proteins); 2FZ7Y3VOQX (Spermine); 9007-49-2 (DNA); 935E97BOY8 (Folic Acid); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160930
[St] Status:MEDLINE


  9 / 117 MEDLINE  
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[PMID]:27404357
[Au] Autor:Chaudhari SN; Mukherjee M; Vagasi AS; Bi G; Rahman MM; Nguyen CQ; Paul L; Selhub J; Kipreos ET
[Ad] Endereço:Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
[Ti] Título:Bacterial Folates Provide an Exogenous Signal for C. elegans Germline Stem Cell Proliferation.
[So] Source:Dev Cell;38(1):33-46, 2016 Jul 11.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here we describe an in vitro primary culture system for Caenorhabditis elegans germline stem cells. This culture system was used to identify a bacterial folate as a positive regulator of germ cell proliferation. Folates are a family of B-complex vitamins that function in one-carbon metabolism to allow the de novo synthesis of amino acids and nucleosides. We show that germ cell proliferation is stimulated by the folate 10-formyl-tetrahydrofolate-Glun both in vitro and in animals. Other folates that can act as vitamins to rescue folate deficiency lack this germ cell stimulatory activity. The bacterial folate precursor dihydropteroate also promotes germ cell proliferation in vitro and in vivo, despite its inability to promote one-carbon metabolism. The folate receptor homolog FOLR-1 is required for the stimulation of germ cells by 10-formyl-tetrahydrofolate-Glun and dihydropteroate. This work defines a folate and folate-related compound as exogenous signals to modulate germ cell proliferation.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/crescimento & desenvolvimento
Proliferação Celular
Escherichia coli/metabolismo
Ácido Fólico/metabolismo
Células Germinativas/citologia
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/metabolismo
Proteínas de Caenorhabditis elegans/genética
Escherichia coli/citologia
Transportadores de Ácido Fólico/genética
Transportadores de Ácido Fólico/metabolismo
Células Germinativas/metabolismo
Células-Tronco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Folic Acid Transporters); 935E97BOY8 (Folic Acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160713
[St] Status:MEDLINE


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[PMID]:27122634
[Au] Autor:Meredith M; MacNeil AH; Trasler JM; Baltz JM
[Ad] Endereço:Ottawa Hospital Research Institute, Ottawa, Ontario, Canada Department of Obstetrics and Gynecology and Department of Cellular and Molecular Medicine, University of Ottawa Faculty of Medicine, Ottawa, Ontario, Canada.
[Ti] Título:Growing Mouse Oocytes Transiently Activate Folate Transport via Folate Receptors As They Approach Full Size.
[So] Source:Biol Reprod;94(6):125, 2016 Jun.
[Is] ISSN:1529-7268
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The folate cycle is central to cellular one-carbon metabolism, where folates are carriers of one-carbon units that are critical for synthesis of purines, thymidylate, and S-adenosylmethionine, the universal methyl donor that forms the cellular methyl pool. Although folates are well-known to be important for early embryo and fetal development, their role in oogenesis has not been clearly established. Here, folate transport proteins were detected in developing neonatal ovaries and growing oocytes by immunohistochemistry, Western blot, and immunofluorescence. The folate receptors FOLR1 and FOLR2 as well as reduced folate carrier 1 (RFC1, SLC19A1 protein) each appeared to be present in follicular cells including granulosa cells. In growing oocytes, however, only FOLR2 immunoreactivity appeared abundant. Localization of apparent FOLR2 immunofluorescence near the plasma membrane increased with oocyte growth and peaked in oocytes as they neared full size. We assessed folate transport using the model folate leucovorin (folinic acid). Unexpectedly, there was a transient burst of folate transport activity for a brief period during oocyte growth as they neared full size, while folate transport was otherwise undetectable for the rest of oogenesis and in fully grown germinal vesicle stage oocytes. This folate transport was inhibited by dynasore, an inhibitor of endocytosis, but insensitive to the anion transport inhibitor stilbene 4-acetamido-40-isothiocyanato-stilbene-2,20-disulfonic acid, consistent with folate receptor-mediated transport but not with RFC1-mediated transport. Thus, near the end of their growth, growing oocytes may take up folates that could support the final stage of oogenesis or be stored to provide the endogenous folates needed in early embryogenesis.
[Mh] Termos MeSH primário: Blastocisto/metabolismo
Transportadores de Ácido Fólico/metabolismo
Ácido Fólico/metabolismo
Oócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Camundongos
Oogênese
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Folic Acid Transporters); 935E97BOY8 (Folic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160429
[St] Status:MEDLINE
[do] DOI:10.1095/biolreprod.115.137687



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