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[PMID]: | 29254296 |
[Au] Autor: | Liu SY; Zhang L; Zhang Y; Zhen Y; Wu YF |
[Ad] Endereço: | Department of Neurosurgery, Second Hospital, Jilin University, Changchun, China. |
[Ti] Título: | Bioinformatic analysis of pivotal genes associated with septic shock. |
[So] Source: | J Biol Regul Homeost Agents;31(4):935-941, 2017 Oct-Dec. | [Is] ISSN: | 0393-974X |
[Cp] País de publicação: | Italy |
[La] Idioma: | eng |
[Ab] Resumo: | We aimed to identify important genes associated with septic shock and then explore the possibly significant mechanisms of this disease. We downloaded GSE26440 expression data of samples from 98 children with septic shock and 32 normal controls from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) in samples from patients with septic shock were analyzed in comparison with those in samples from normal controls using a limma package. Functional enrichment analysis for DEGs was performed using DAVID, and a protein–protein interaction (PPI) network was constructed. Upstream transcription factors for DEGs were predicted using the CHIPBase database, and a transcriptional regulation network was constructed. A total of 383 significantly DEGs, including 141 downregulated and 242 upregulated genes, were obtained in the sepsis shock group compared with the normal group. The top five nodes in the PPI network were lysine (K)-specific demethylase 6B (KDM6B), histone deacetylase 2 (HDAC2), V-Myc avian myelocytomatosis viral oncogene homolog (MYC), heat-shock protein 90 kDa alpha (cytosolic), class B member 1 (HSP90AB1), and poly (A)-binding protein, cytoplasmic 1 (PABPC1). Nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) was the transcription factor targeted by most genes, and it regulated the expression of KDM6B, HDAC2, MYC, HSP90AB1, and PABPC1. In conclusion, KDM6B, HDAC2, MYC, HSP90AB1, and PABPC1 may play important roles in the development of septic shock. Furthermore, NFκB may be involved in septic shock by regulating the expression of KDM6B, HDAC2, MYC, HSP90AB1, and PABPC1. |
[Mh] Termos MeSH primário: |
Biologia Computacional/métodos Redes Reguladoras de Genes NF-kappa B/genética Choque Séptico/genética Transcriptoma
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[Mh] Termos MeSH secundário: |
Estudos de Casos e Controles Criança Pré-Escolar Feminino Perfilação da Expressão Gênica Regulação da Expressão Gênica Proteínas de Choque Térmico HSP90/genética Proteínas de Choque Térmico HSP90/metabolismo Histona Desacetilase 2/genética Histona Desacetilase 2/metabolismo Seres Humanos Histona Desmetilases com o Domínio Jumonji/genética Histona Desmetilases com o Domínio Jumonji/metabolismo Masculino NF-kappa B/metabolismo Proteína I de Ligação a Poli(A)/genética Proteína I de Ligação a Poli(A)/metabolismo Mapeamento de Interação de Proteínas Proteínas Proto-Oncogênicas c-myc/genética Proteínas Proto-Oncogênicas c-myc/metabolismo Choque Séptico/metabolismo Choque Séptico/patologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (HSP90 Heat-Shock Proteins); 0 (HSP90AB1 protein, human); 0 (MYC protein, human); 0 (NF-kappa B); 0 (Poly(A)-Binding Protein I); 0 (Proto-Oncogene Proteins c-myc); EC 1.14.11.- (Jumonji Domain-Containing Histone Demethylases); EC 1.14.11.- (KDM6B protein, human); EC 3.5.1.98 (HDAC2 protein, human); EC 3.5.1.98 (Histone Deacetylase 2) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180222 |
[Lr] Data última revisão:
| 180222 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171220 |
[St] Status: | MEDLINE |
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