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Pesquisa : D12.776.157.725.500.750.800 [Categoria DeCS]
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[PMID]:28862756
[Au] Autor:Haßdenteufel S; Sicking M; Schorr S; Aviram N; Fecher-Trost C; Schuldiner M; Jung M; Zimmermann R; Lang S
[Ad] Endereço:Department of Medical Biochemistry and Molecular Biology, Saarland University, Homburg, Germany.
[Ti] Título:hSnd2 protein represents an alternative targeting factor to the endoplasmic reticulum in human cells.
[So] Source:FEBS Lett;591(20):3211-3224, 2017 Oct.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recently, understanding of protein targeting to the endoplasmic reticulum (ER) was expanded by the discovery of multiple pathways that function in parallel to the signal recognition particle (SRP). Guided entry of tail-anchored proteins and SRP independent (SND) are two such targeting pathways described in yeast. So far, no human SND component is functionally characterized. Here, we report hSnd2 as the first constituent of the human SND pathway able to support substrate-specific protein targeting to the ER. Similar to its yeast counterpart, hSnd2 is assumed to function as a membrane-bound receptor preferentially targeting precursors carrying C-terminal transmembrane domains. Our genetic and physical interaction studies show that hSnd2 is part of a complex network of targeting and translocation that is dynamically regulated.
[Mh] Termos MeSH primário: Retículo Endoplasmático/metabolismo
Proteínas de Membrana/genética
Proteínas Nucleares/genética
Subunidades Proteicas/genética
Receptores Citoplasmáticos e Nucleares/genética
Receptores de Peptídeos/genética
Canais de Translocação SEC/genética
Proteínas de Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Regulação da Expressão Gênica
Células HeLa
Seres Humanos
Proteínas de Membrana/antagonistas & inibidores
Proteínas de Membrana/metabolismo
Proteínas Nucleares/metabolismo
Peptídeos/síntese química
Peptídeos/metabolismo
Ligação Proteica
Isoformas de Proteínas/antagonistas & inibidores
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Precursores de Proteínas/genética
Precursores de Proteínas/metabolismo
Sinais Direcionadores de Proteínas/genética
Subunidades Proteicas/metabolismo
Transporte Proteico
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Coelhos
Receptores Citoplasmáticos e Nucleares/metabolismo
Receptores de Peptídeos/metabolismo
Canais de Translocação SEC/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/antagonistas & inibidores
Proteínas de Saccharomyces cerevisiae/metabolismo
Partícula de Reconhecimento de Sinal
Transdução de Sinais
Especificidade por Substrato
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Membrane Proteins); 0 (Nuclear Proteins); 0 (Peptides); 0 (Protein Isoforms); 0 (Protein Precursors); 0 (Protein Sorting Signals); 0 (Protein Subunits); 0 (RNA, Small Interfering); 0 (Receptors, Cytoplasmic and Nuclear); 0 (Receptors, Peptide); 0 (SEC Translocation Channels); 0 (Saccharomyces cerevisiae Proteins); 0 (Signal Recognition Particle); 0 (Snd2 protein, S cerevisiae); 0 (WRB protein, human); 0 (signal peptide receptor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170902
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12831


  2 / 1120 MEDLINE  
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[PMID]:28847176
[Au] Autor:Botos B; Nagy-Vincze M; Dankó K
[Ad] Endereço:I. Belgyógyászati Osztály, Borsod-Abaúj-Zemplén Megyei Központi Kórház és Egyetemi Oktatókórház Miskolc.
[Ti] Título:[Clinical features and therapeutic response of our anti-SRP positive patients with myositis].
[Ti] Título:Anti-SRP-pozitív myositises betegeink klinikai sajátosságai és terápiára adott válaszuk..
[So] Source:Orv Hetil;158(35):1382-1389, 2017 Sep.
[Is] ISSN:0030-6002
[Cp] País de publicação:Hungary
[La] Idioma:hun
[Ab] Resumo:INTRODUCTION: Idiopathic inflammatory myopathies are a group of clinically heterogeneous diseases, which have been classified by myositis specific antibodies recently. The anti-SRP positive subset of this group is characterized by more severe clinical prognosis than other myositis specific antibody positive types. AIM: Our goal was to compare 16 anti-SRP positive patients in the Division of Clinical Immunology, Department of Internal Medicine, University of Debrecen with 16 antibody negative ones. METHOD: Muscle strength validated in both groups by the manual muscle test proved to be significantly decreased both before and after therapy (χ = 0.006 and 0.019) in the anti-SRP positive group. RESULTS: Muscle-specific inflammatory laboratory parameters showed significant difference only in case of LDH-levels after therapy. Both groups showed good clinical response to first line steroid treatment, yet the significantly higher rate of second line administration suggests worse therapeutic response of the antibody positive group. CONCLUSION: Based on these facts we determined poor clinical prognosis and therapeutic response of the anti-SRP positive group. Orv Hetil. 2017; 158(35): 1382-1389.
[Mh] Termos MeSH primário: Autoanticorpos/imunologia
Músculo Esquelético/imunologia
Miosite/imunologia
Partícula de Reconhecimento de Sinal/imunologia
[Mh] Termos MeSH secundário: Adulto
Anti-Inflamatórios/uso terapêutico
Feminino
Seres Humanos
Masculino
Meia-Idade
Músculo Esquelético/patologia
Miosite/tratamento farmacológico
Miosite/patologia
Prognóstico
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Autoantibodies); 0 (Signal Recognition Particle)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1556/650.2017.30827


  3 / 1120 MEDLINE  
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[PMID]:28709002
[Au] Autor:Nabet BY; Qiu Y; Shabason JE; Wu TJ; Yoon T; Kim BC; Benci JL; DeMichele AM; Tchou J; Marcotrigiano J; Minn AJ
[Ad] Endereço:Department of Radiation Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
[Ti] Título:Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.
[So] Source:Cell;170(2):352-366.e13, 2017 Jul 13.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Interactions between stromal fibroblasts and cancer cells generate signals for cancer progression, therapy resistance, and inflammatory responses. Although endogenous RNAs acting as damage-associated molecular patterns (DAMPs) for pattern recognition receptors (PRRs) may represent one such signal, these RNAs must remain unrecognized under non-pathological conditions. We show that triggering of stromal NOTCH-MYC by breast cancer cells results in a POL3-driven increase in RN7SL1, an endogenous RNA normally shielded by RNA binding proteins SRP9/14. This increase in RN7SL1 alters its stoichiometry with SRP9/14 and generates unshielded RN7SL1 in stromal exosomes. After exosome transfer to immune cells, unshielded RN7SL1 drives an inflammatory response. Upon transfer to breast cancer cells, unshielded RN7SL1 activates the PRR RIG-I to enhance tumor growth, metastasis, and therapy resistance. Corroborated by evidence from patient tumors and blood, these results demonstrate that regulation of RNA unshielding couples stromal activation with deployment of RNA DAMPs that promote aggressive features of cancer. VIDEO ABSTRACT.
[Mh] Termos MeSH primário: Neoplasias da Mama/patologia
Exossomos/patologia
RNA não Traduzido/metabolismo
Células Estromais/patologia
Microambiente Tumoral
[Mh] Termos MeSH secundário: Neoplasias da Mama/metabolismo
Proteína DEAD-box 58/metabolismo
Exossomos/metabolismo
Seres Humanos
Fatores Reguladores de Interferon/metabolismo
Células MCF-7
Metástase Neoplásica
RNA Polimerase III/genética
RNA Polimerase III/metabolismo
Receptores de Reconhecimento de Padrão/metabolismo
Partícula de Reconhecimento de Sinal/metabolismo
Células Estromais/metabolismo
Viroses/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interferon Regulatory Factors); 0 (RNA, Untranslated); 0 (Receptors, Pattern Recognition); 0 (SRP14 protein, human); 0 (SRP9 protein, human); 0 (Signal Recognition Particle); EC 2.7.7.6 (RNA Polymerase III); EC 3.6.1.- (DDX58 protein, human); EC 3.6.4.13 (DEAD Box Protein 58)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE


  4 / 1120 MEDLINE  
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[PMID]:28704369
[Au] Autor:Martínez-Valencia AJ; Daza-Rivera CF; Rosales-Chilama M; Cossio A; Casadiego Rincón EJ; Desai MM; Saravia NG; Gómez MA
[Ad] Endereço:Centro Internacional de Entrenamiento e Investigaciones Médicas-CIDEIM, Cali, Colombia.
[Ti] Título:Clinical and parasitological factors in parasite persistence after treatment and clinical cure of cutaneous leishmaniasis.
[So] Source:PLoS Negl Trop Dis;11(7):e0005713, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The determinants of parasite persistence or elimination after treatment and clinical resolution of cutaneous leishmaniasis (CL) are unknown. We investigated clinical and parasitological parameters associated with the presence and viability of Leishmania after treatment and resolution of CL caused by L. Viannia. METHODS: Seventy patients who were treated with meglumine antimoniate (n = 38) or miltefosine (n = 32) and cured, were included in this study. Leishmania persistence and viability were determined by detection of kDNA and 7SLRNA transcripts, respectively, before, at the end of treatment (EoT), and 13 weeks after initiation of treatment in lesions and swabs of nasal and tonsillar mucosa. RESULTS: Sixty percent of patients (42/70) had evidence of Leishmania persistence at EoT and 30% (9/30) 13 weeks after treatment initiation. A previous episode of CL was found to be a protective factor for detectable Leishmania persistence (OR: 0.16, 95%CI: 0.03-0.92). kDNA genotyping could not discern differences between parasite populations that persisted and those isolated at diagnosis. CONCLUSIONS: Leishmania persist in skin and mucosal tissues in a high proportion of patients who achieved therapeutic cure of CL. This finding prompts assessment of the contribution of persistent infection in transmission and endemicity of CL, and in disease reactivation and protective immunity.
[Mh] Termos MeSH primário: Leishmania/isolamento & purificação
Leishmaniose Cutânea/tratamento farmacológico
Leishmaniose Cutânea/parasitologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
DNA de Cinetoplasto/análise
DNA de Protozoário/análise
Feminino
Seguimentos
Seres Humanos
Leishmania/fisiologia
Masculino
Meia-Idade
Membrana Mucosa/parasitologia
Estudos Prospectivos
RNA de Protozoário/análise
RNA Citoplasmático Pequeno/análise
Partícula de Reconhecimento de Sinal/análise
Pele/parasitologia
Análise de Sobrevida
Fatores de Tempo
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (7SL RNA); 0 (DNA, Kinetoplast); 0 (DNA, Protozoan); 0 (RNA, Protozoan); 0 (RNA, Small Cytoplasmic); 0 (Signal Recognition Particle)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005713


  5 / 1120 MEDLINE  
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[PMID]:28696227
[Au] Autor:Nordholm J; Petitou J; Östbye H; da Silva DV; Dou D; Wang H; Daniels R
[Ad] Endereço:Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
[Ti] Título:Translational regulation of viral secretory proteins by the 5' coding regions and a viral RNA-binding protein.
[So] Source:J Cell Biol;216(8):2283-2293, 2017 Aug 07.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A primary function of 5' regions in many secretory protein mRNAs is to encode an endoplasmic reticulum (ER) targeting sequence. In this study, we show how the regions coding for the ER-targeting sequences of the influenza glycoproteins NA and HA also function as translational regulatory elements that are controlled by the viral RNA-binding protein (RBP) NS1. The translational increase depends on the nucleotide composition and 5' positioning of the ER-targeting sequence coding regions and is facilitated by the RNA-binding domain of NS1, which can associate with ER membranes. Inserting the ER-targeting sequence coding region of NA into different 5' UTRs confirmed that NS1 can promote the translation of secretory protein mRNAs based on the nucleotides within this region rather than the resulting amino acids. By analyzing human protein mRNA sequences, we found evidence that this mechanism of using 5' coding regions and particular RBPs to achieve gene-specific regulation may extend to human-secreted proteins.
[Mh] Termos MeSH primário: Glicoproteínas de Hemaglutininação de Vírus da Influenza/biossíntese
Vírus da Influenza A Subtipo H1N1/enzimologia
Neuraminidase/metabolismo
RNA Mensageiro/metabolismo
RNA Viral/metabolismo
Proteínas não Estruturais Virais/metabolismo
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
Regiões 5' não Traduzidas
Células A549
Animais
Sítios de Ligação
Células COS
Cercopithecus aethiops
Retículo Endoplasmático/enzimologia
Células HEK293
Células HeLa
Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética
Seres Humanos
Vírus da Influenza A Subtipo H1N1/genética
Neuraminidase/genética
Ligação Proteica
Biossíntese de Proteínas
Domínios Proteicos
RNA Mensageiro/genética
RNA Viral/genética
Partícula de Reconhecimento de Sinal/genética
Partícula de Reconhecimento de Sinal/metabolismo
Transfecção
Células Vero
Proteínas não Estruturais Virais/genética
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (5' Untranslated Regions); 0 (H1N1 virus hemagglutinin); 0 (Hemagglutinin Glycoproteins, Influenza Virus); 0 (INS1 protein, influenza virus); 0 (RNA, Messenger); 0 (RNA, Viral); 0 (Signal Recognition Particle); 0 (Viral Nonstructural Proteins); 0 (Viral Proteins); EC 3.2.1.18 (NA protein, influenza A virus); EC 3.2.1.18 (Neuraminidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201702102


  6 / 1120 MEDLINE  
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[PMID]:28367947
[Au] Autor:Kawakami N; Katsuyama Y; Hagiwara Y; Yoshida H; Kim K; Harada K
[Ad] Endereço:Department of Neurology, Shizuoka General Hospital.
[Ti] Título:A case of amyloid myopathy diagnosed during the treatment of myopathy associated with anti-signal recognition particle antibodies.
[So] Source:Rinsho Shinkeigaku;57(4):168-173, 2017 04 28.
[Is] ISSN:1882-0654
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:A 78-year-old man presented with subacute progressive proximal weakness and dysphagia. A biopsy specimen from the left biceps femoris revealed evidence of necrotic and regenerating muscle fibers, but lymphocyte infiltration was not noted. The patient was diagnosed with necrotizing myopathy with anti-signal recognition particle (SRP) antibodies. Concomitant therapy with prednisolone and azathioprine caused the serum CK level to return to normal and it caused clinical manifestations to abate. One year later, however, muscle weakness worsened. Immunoelectrophoresis of serum revealed IgG M protein, and muscle pathology revealed amyloid deposits in numerous blood vessels and at the periphery of a few muscle fibers, and deposits stained positive for anti-λ light chain antibody. The patient was diagnosed with amyloid myopathy, and therapy for systemic amyloid light chain amyloidosis caused muscle weakness to diminish. Amyloidosis is believed to be the primary pathology in this case based on the patient's response to treatment reaction, but the significance of a case involving both amyloid myopathy and necrotizing myopathy warranted examination.
[Mh] Termos MeSH primário: Amiloidose/diagnóstico
Autoanticorpos/sangue
Doenças Musculares/diagnóstico
Partícula de Reconhecimento de Sinal/imunologia
[Mh] Termos MeSH secundário: Idoso
Amiloide/metabolismo
Amiloidose/tratamento farmacológico
Amiloidose/patologia
Biomarcadores/sangue
Biomarcadores/metabolismo
Glicoproteínas/sangue
Seres Humanos
Imunoglobulina G/sangue
Cadeias lambda de Imunoglobulina/metabolismo
Imagem por Ressonância Magnética
Masculino
Músculos/diagnóstico por imagem
Músculos/metabolismo
Músculos/patologia
Doenças Musculares/tratamento farmacológico
Doenças Musculares/patologia
Necrose
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amyloid); 0 (Autoantibodies); 0 (Biomarkers); 0 (Glycoproteins); 0 (Immunoglobulin G); 0 (Immunoglobulin lambda-Chains); 0 (Signal Recognition Particle); 0 (protein M (glycoprotein))
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.5692/clinicalneurol.cn-000974


  7 / 1120 MEDLINE  
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[PMID]:28224701
[Au] Autor:Arouche-Delaperche L; Allenbach Y; Amelin D; Preusse C; Mouly V; Mauhin W; Tchoupou GD; Drouot L; Boyer O; Stenzel W; Butler-Browne G; Benveniste O
[Ad] Endereço:Pierre and Marie Curie University, Sorbonne Universities, National Institute of Health and Medical Research, National Center for Scientific Research, Myology Research Center, Pitié-Salpêtrière University Hospital, Paris, France.
[Ti] Título:Pathogenic role of anti-signal recognition protein and anti-3-Hydroxy-3-methylglutaryl-CoA reductase antibodies in necrotizing myopathies: Myofiber atrophy and impairment of muscle regeneration in necrotizing autoimmune myopathies.
[So] Source:Ann Neurol;81(4):538-548, 2017 Apr.
[Is] ISSN:1531-8249
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Immune-mediated necrotizing myopathies (IMNM) may be associated with either anti-signal recognition protein (SRP) or anti-3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) antibodies (Abs), and the titer of these Abs is correlated with disease activity. We investigated whether anti-SRP and anti-HMGCR Abs could be involved in muscle damage. METHODS: Muscle biopsies of patients were analyzed for atrophy and regeneration by measuring fiber size and by performing immunostaining of neonatal myosin heavy chain. To further understand the role of the Abs in the pathology, we performed muscle cell coculture with the Abs. Atrophy and regeneration were evaluated based on the myotube surface area as well as gene and cytokine profiles. RESULTS: In muscle biopsies of patients with anti-SRP and anti-HMGCR Abs, a large number of small fibers corresponding to both atrophic and regenerating fibers were observed. In vitro, anti-SRP and anti-HMGCR Abs induced muscle fiber atrophy and increased the transcription of MAFbx and TRIM63. In addition, the muscle fiber atrophy was associated with high levels of inflammatory cytokines: tumor necrosis factor, interleukin (IL)-6, and reactive oxygen species. In the presence of anti-SRP or anti-HMGCR Abs, mechanisms involved in muscle regeneration were also impaired due to a defect of myoblast fusion. This defect was associated with a decreased production of IL-4 and IL-13. The addition of IL-4 and/or IL-13 totally rescued fusion capacity. INTERPRETATION: These data show that molecular mechanisms of atrophy and regeneration are affected and contribute to loss of muscle function occurring in IMNM. This emphasizes the potential interest of targeted therapies addressing these mechanisms. Ann Neurol 2017;81:538-548.
[Mh] Termos MeSH primário: Autoanticorpos/imunologia
Doenças Autoimunes
Hidroximetilglutaril-CoA Redutases/imunologia
Fibras Musculares Esqueléticas
Doenças Musculares
Regeneração/fisiologia
Partícula de Reconhecimento de Sinal/imunologia
Bancos de Tecidos
[Mh] Termos MeSH secundário: Atrofia/patologia
Doenças Autoimunes/imunologia
Doenças Autoimunes/patologia
Doenças Autoimunes/fisiopatologia
Técnicas de Cultura de Células
Seres Humanos
Fibras Musculares Esqueléticas/imunologia
Fibras Musculares Esqueléticas/patologia
Fibras Musculares Esqueléticas/fisiologia
Doenças Musculares/imunologia
Doenças Musculares/patologia
Doenças Musculares/fisiopatologia
Necrose/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autoantibodies); 0 (Signal Recognition Particle); EC 1.1.1.- (HMGCR protein, human); EC 1.1.1.- (Hydroxymethylglutaryl CoA Reductases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1002/ana.24902


  8 / 1120 MEDLINE  
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[PMID]:28129347
[Au] Autor:Russo J; Lee JE; López CM; Anderson J; Nguyen TP; Heck AM; Wilusz J; Wilusz CJ
[Ad] Endereço:Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.
[Ti] Título:The CELF1 RNA-Binding Protein Regulates Decay of Signal Recognition Particle mRNAs and Limits Secretion in Mouse Myoblasts.
[So] Source:PLoS One;12(1):e0170680, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We previously identified several mRNAs encoding components of the secretory pathway, including signal recognition particle (SRP) subunit mRNAs, among transcripts associated with the RNA-binding protein CELF1. Through immunoprecipitation of RNAs crosslinked to CELF1 in myoblasts and in vitro binding assays using recombinant CELF1, we now provide evidence that CELF1 directly binds the mRNAs encoding each of the subunits of the SRP. Furthermore, we determined the half-lives of the Srp transcripts in control and CELF1 knockdown myoblasts. Our results indicate CELF1 is a destabilizer of at least five of the six Srp transcripts and that the relative abundance of the SRP proteins is out of balance when CELF1 is depleted. CELF1 knockdown myoblasts exhibit altered secretion of a luciferase reporter protein and are impaired in their ability to migrate and close a wound, consistent with a defect in the secreted extracellular matrix. Importantly, similar defects in wound healing are observed when SRP subunit imbalance is induced by over-expression of SRP68. Our studies support the existence of an RNA regulon containing Srp mRNAs that is controlled by CELF1. One implication is that altered function of CELF1 in myotonic dystrophy may contribute to changes in the extracellular matrix of affected muscle through defects in secretion.
[Mh] Termos MeSH primário: Proteínas CELF1/genética
Estabilidade de RNA/genética
Proteínas de Ligação a RNA/genética
Partícula de Reconhecimento de Sinal/genética
[Mh] Termos MeSH secundário: Animais
Camundongos
Mioblastos/metabolismo
RNA/genética
RNA/metabolismo
RNA Mensageiro/genética
Partícula de Reconhecimento de Sinal/metabolismo
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CELF1 Protein); 0 (CELF1 protein, mouse); 0 (RNA, Messenger); 0 (RNA-Binding Proteins); 0 (Signal Recognition Particle); 63231-63-0 (RNA)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170680


  9 / 1120 MEDLINE  
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[PMID]:28086075
[Au] Autor:Zhao G; Hou J; Xu G; Xiang A; Kang Y; Yan Y; Zhang X; Yang G; Xiao S; Sun S
[Ad] Endereço:1​College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, PR China.
[Ti] Título:Cellular microRNA miR-10a-5p inhibits replication of porcine reproductive and respiratory syndrome virus by targeting the host factor signal recognition particle 14.
[So] Source:J Gen Virol;98(4):624-632, 2017 Apr.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important viruses affecting the swine industry worldwide. MicroRNAs have recently been demonstrated to play vital roles in virus-host interactions. Our previous research on small RNA deep sequencing showed that the expression level of miR-10a increased during the viral life cycle. The present study sought to determine the function of miR-10a and its molecular mechanism during PRRSV infection. In the current study, the result of PRRSV infection inducing miR-10a expression was validated by quantitative reverse transcriptase PCR. Overexpression of miR-10a-5p using its mimics markedly reduced the expression level of intracellular PRRSV ORF7 mRNA and N protein. Simultaneously, overexpression of miR-10a-5p also significantly decreased the expression level of extracellular viral RNA and virus titres in the supernatants. These results demonstrated that miR-10a-5p could suppress the replication of PRRSV. A direct interaction between miR-10a-5p and signal recognition particle 14 (SRP14) was confirmed using bioinformatic prediction and experimental verification. miR-10a-5p could directly target the 3'UTR of pig SRP14 mRNA in a sequence-specific manner and decrease SRP14 expression through translational repression but not mRNA degradation. Further, knockdown of SRP14 by small interfering RNA also inhibits the replication of PRRSV. Collectively, these results suggested that miR-10a-5p inhibits PRRSV replication through suppression of SRP14 expression, which not only provides new insights into virus-host interactions during PRRSV infection but also suggests potential new antiviral strategies against PRRSV infection.
[Mh] Termos MeSH primário: Interações Hospedeiro-Patógeno
MicroRNAs/metabolismo
Vírus da Síndrome Respiratória e Reprodutiva Suína/imunologia
Vírus da Síndrome Respiratória e Reprodutiva Suína/fisiologia
Partícula de Reconhecimento de Sinal/antagonistas & inibidores
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Perfilação da Expressão Gênica
MicroRNAs/biossíntese
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Suínos
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Signal Recognition Particle)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000708


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[PMID]:28076289
[Au] Autor:Ziehe D; Dünschede B; Schünemann D
[Ad] Endereço:Molecular Biology of Plant Organelles, Ruhr University Bochum, Universitätsstraße 150, D-44780 Bochum.
[Ti] Título:From bacteria to chloroplasts: evolution of the chloroplast SRP system.
[So] Source:Biol Chem;398(5-6):653-661, 2017 May 01.
[Is] ISSN:1437-4315
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Chloroplasts derive from a prokaryotic symbiont that lost most of its genes during evolution. As a result, the great majority of chloroplast proteins are encoded in the nucleus and are posttranslationally imported into the organelle. The chloroplast genome encodes only a few proteins. These include several multispan thylakoid membrane proteins which are synthesized on thylakoid-bound ribosomes and cotranslationally inserted into the membrane. During evolution, ancient prokaryotic targeting machineries were adapted and combined with novel targeting mechanisms to facilitate post- and cotranslational protein transport in chloroplasts. This review focusses on the chloroplast signal recognition particle (cpSRP) protein transport system, which has been intensively studied in higher plants. The cpSRP system derived from the prokaryotic SRP pathway, which mediates the cotranslational protein transport to the bacterial plasma membrane. Chloroplasts contain homologs of several components of the bacterial SRP system. The function of these conserved components in post- and/or cotranslational protein transport and chloroplast-specific modifications of these transport mechanisms are described. Furthermore, recent studies of cpSRP systems in algae and lower plants are summarized and their impact on understanding the evolution of the cpSRP system are discussed.
[Mh] Termos MeSH primário: Bactérias/citologia
Bactérias/metabolismo
Cloroplastos/metabolismo
Evolução Molecular
Partícula de Reconhecimento de Sinal/metabolismo
[Mh] Termos MeSH secundário: Filogenia
Partícula de Reconhecimento de Sinal/química
Partícula de Reconhecimento de Sinal/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Signal Recognition Particle)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE



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