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[PMID]:29247589
[Au] Autor:Udagawa C; Nakamura H; Ohnishi H; Tamura K; Shimoi T; Yoshida M; Yoshida T; Totoki Y; Shibata T; Zembutsu H
[Ad] Endereço:Division of Genetics, National Cancer Center Research Institute, Tokyo, Japan.
[Ti] Título:Whole exome sequencing to identify genetic markers for trastuzumab-induced cardiotoxicity.
[So] Source:Cancer Sci;109(2):446-452, 2018 Feb.
[Is] ISSN:1349-7006
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although trastuzumab-induced cardiotoxicity is an important determinant to limit the use of this drug, the molecular mechanism of risk for this toxicity is not well understood. To identify genetic variants determining the risk of trastuzumab-induced cardiotoxicity, we carried out whole exome sequencing of germline DNA samples from 9 patients with trastuzumab-induced cardiotoxicity, and conducted a case-control association study of 2258 genetic variants between 9 cases (with trastuzumab-induced cardiotoxicity) and general Japanese population controls registered in the Human Genetic Variation Database (HGVD). The top variant which showed the lowest P-value in the screening study was rs139503277 in PHD Finger Protein 3 (P = .00012, odds ratio [OR] = 51.23). To further validate the result of screening study, we carried out a replication study of 10 variants showing P < .001 in the screening study using 234 independent patients treated with trastuzumab, including 10 cases and 224 controls (without trastuzumab-induced cardiotoxicity). In the replication study, we observed that three variants had an effect in the same direction as in the screening study (rs78272919 in exon 2 of Keratin 15, rs5762940 in exon 2 of zinc and ring finger 3, and rs139944387 in exon 44 of Eyes shut homologs [EYS]). A combined result of the screening and the replication studies suggested an association of a locus on chromosome 6q12 with trastuzumab-induced cardiotoxicity (rs139944387 in EYS, combined P = .00056, OR = 13.73). This finding provides new insights into personalized trastuzumab therapy for patients with human epidermal growth factor receptor 2 (HER2)-positive cancer.
[Mh] Termos MeSH primário: Cardiotoxicidade/genética
Marcadores Genéticos/genética
Polimorfismo de Nucleotídeo Único
Trastuzumab/toxicidade
Sequenciamento Completo do Exoma/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Estudos de Casos e Controles
Proteínas do Olho/genética
Feminino
Mutação em Linhagem Germinativa
Seres Humanos
Queratina-15/genética
Masculino
Meia-Idade
Fatores de Transcrição/genética
Ubiquitina-Proteína Ligases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (EYS protein, human); 0 (Eye Proteins); 0 (Genetic Markers); 0 (KRT15 protein, human); 0 (Keratin-15); 0 (PHF3 protein, human); 0 (Transcription Factors); EC 2.3.2.27 (Ubiquitin-Protein Ligases); EC 2.3.2.27 (ZNRF3 protein, human); P188ANX8CK (Trastuzumab)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1111/cas.13471


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[PMID]:28481227
[Au] Autor:Giroux V; Lento AA; Islam M; Pitarresi JR; Kharbanda A; Hamilton KE; Whelan KA; Long A; Rhoades B; Tang Q; Nakagawa H; Lengner CJ; Bass AJ; Wileyto EP; Klein-Szanto AJ; Wang TC; Rustgi AK
[Ad] Endereço:Division of Gastroenterology, Department of Medicine, and.
[Ti] Título:Long-lived keratin 15+ esophageal progenitor cells contribute to homeostasis and regeneration.
[So] Source:J Clin Invest;127(6):2378-2391, 2017 Jun 01.
[Is] ISSN:1558-8238
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The esophageal lumen is lined by a stratified squamous epithelium comprised of proliferative basal cells that differentiate while migrating toward the luminal surface and eventually desquamate. Rapid epithelial renewal occurs, but the specific cell of origin that supports this high proliferative demand remains unknown. Herein, we have described a long-lived progenitor cell population in the mouse esophageal epithelium that is characterized by expression of keratin 15 (Krt15). Genetic in vivo lineage tracing revealed that the Krt15 promoter marks a long-lived basal cell population able to self-renew, proliferate, and generate differentiated cells, consistent with a progenitor/stem cell population. Transcriptional profiling demonstrated that Krt15+ basal cells are molecularly distinct from Krt15- basal cells. Depletion of Krt15-derived cells resulted in decreased proliferation, thereby leading to atrophy of the esophageal epithelium. Further, Krt15+ cells were radioresistant and contributed to esophageal epithelial regeneration following radiation-induced injury. These results establish the presence of a long-lived and indispensable Krt15+ progenitor cell population that provides additional perspective on esophageal epithelial biology and the widely prevalent diseases that afflict this epithelium.
[Mh] Termos MeSH primário: Esôfago/citologia
Queratina-15/metabolismo
Células-Tronco/fisiologia
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Movimento Celular
Proliferação Celular
Sobrevivência Celular/efeitos da radiação
Esôfago/fisiologia
Esôfago/efeitos da radiação
Homeostase
Seres Humanos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Membrana Mucosa/citologia
Regiões Promotoras Genéticas
Lesões Experimentais por Radiação/fisiopatologia
Regeneração
Células-Tronco/efeitos da radiação
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Keratin-15); 0 (Krt1-15 protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE


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[PMID]:28331065
[Au] Autor:Kullmann FA; Clayton DR; Ruiz WG; Wolf-Johnston A; Gauthier C; Kanai A; Birder LA; Apodaca G
[Ad] Endereço:University of Pittsburgh School of Medicine, Department of Medicine, Pittsburgh, Pennsylvania.
[Ti] Título:Urothelial proliferation and regeneration after spinal cord injury.
[So] Source:Am J Physiol Renal Physiol;313(1):F85-F102, 2017 Jul 01.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The basal, intermediate, and superficial cell layers of the urothelium undergo rapid and complete recovery following acute injury; however, the effects of chronic injury on urothelial regeneration have not been well defined. To address this discrepancy, we employed a mouse model to explore urothelial changes in response to spinal cord injury (SCI), a condition characterized by life-long bladder dysfunction. One day post SCI there was a focal loss of umbrella cells, which are large cells that populate the superficial cell layer and normally express uroplakins (UPKs) and KRT20, but not KRT5, KRT14, or TP63. In response to SCI, regions of urothelium devoid of umbrella cells were replaced with small superficial cells that lacked KRT20 expression and appeared to be derived in part from the underlying intermediate cell layer, including cells positive for KRT5 and TP63. We also observed KRT14-positive basal cells that extended thin cytoplasmic extensions, which terminated in the bladder lumen. Both KRT14-positive and KRT14-negative urothelial cells proliferated 1 day post SCI, and by 7 days, cells in the underlying lamina propria, detrusor, and adventitia were also dividing. At 28 days post SCI, the urothelium appeared morphologically patent, and the number of proliferative cells decreased to baseline levels; however, patches of small superficial cells were detected that coexpressed UPKs, KRT5, KRT14, and TP63, but failed to express KRT20. Thus, unlike the rapid and complete restoration of the urothelium that occurs in response to acute injuries, regions of incompletely differentiated urothelium were observed even 28 days post SCI.
[Mh] Termos MeSH primário: Proliferação Celular
Regeneração
Traumatismos da Medula Espinal/patologia
Bexiga Urinária/patologia
Urotélio/patologia
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Modelos Animais de Doenças
Feminino
Queratina-14/metabolismo
Queratina-15/metabolismo
Queratina-20/metabolismo
Camundongos Endogâmicos C57BL
Fenótipo
Fosfoproteínas/metabolismo
Traumatismos da Medula Espinal/metabolismo
Traumatismos da Medula Espinal/fisiopatologia
Fatores de Tempo
Transativadores/metabolismo
Bexiga Urinária/inervação
Bexiga Urinária/metabolismo
Bexiga Urinária/ultraestrutura
Urotélio/inervação
Urotélio/metabolismo
Urotélio/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Keratin-14); 0 (Keratin-15); 0 (Keratin-20); 0 (Krt1-14 protein, mouse); 0 (Krt1-15 protein, mouse); 0 (Krt20 protein, mouse); 0 (Phosphoproteins); 0 (Trans-Activators); 0 (Trp63 protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00592.2016


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[PMID]:28325419
[Au] Autor:Kolivras A; Thompson C
[Ad] Endereço:Department of Dermatology, Saint-Pierre, Brugmann and Queen Fabiola Children University Hospitals, Université Libre de Bruxelles, Brussels, Belgium; Department of Dermatopathology, Saint-Pierre, Brugmann and Queen Fabiola Children University Hospitals, Université Libre de Bruxelles, Brussels, Belgium. Electronic address: kolivras@gmail.com.
[Ti] Título:Reply to: "Lack of specificity of cytokeratin-15 loss in scarring alopecias".
[So] Source:J Am Acad Dermatol;76(4):e137-e138, 2017 04.
[Is] ISSN:1097-6787
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Cicatriz
Queratina-15
[Mh] Termos MeSH secundário: Alopecia
Folículo Piloso
Seres Humanos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:LETTER; COMMENT
[Nm] Nome de substância:
0 (Keratin-15)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE


  5 / 218 MEDLINE  
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[PMID]:28325418
[Au] Autor:Mahalingam M
[Ad] Endereço:Dermatopathology Section, Veterans Affairs Medical Center (VISN1), West Roxbury, Massachusetts. Electronic address: me@meeramahalingam.com.
[Ti] Título:Lack of specificity of cytokeratin-15 loss in scarring alopecias.
[So] Source:J Am Acad Dermatol;76(4):e135-e136, 2017 04.
[Is] ISSN:1097-6787
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Cicatriz
Queratina-15
[Mh] Termos MeSH secundário: Alopecia
Folículo Piloso
Seres Humanos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:LETTER; COMMENT
[Nm] Nome de substância:
0 (Keratin-15)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE


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[PMID]:27869817
[Au] Autor:Hidaka T; Ogawa E; Kobayashi EH; Suzuki T; Funayama R; Nagashima T; Fujimura T; Aiba S; Nakayama K; Okuyama R; Yamamoto M
[Ad] Endereço:Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Japan.
[Ti] Título:The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin.
[So] Source:Nat Immunol;18(1):64-73, 2017 Jan.
[Is] ISSN:1529-2916
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Atopic dermatitis is increasing worldwide in correlation with air pollution. Various organic components of pollutants activate the transcription factor AhR (aryl hydrocarbon receptor). Through the use of AhR-CA mice, whose keratinocytes express constitutively active AhR and that develop atopic-dermatitis-like phenotypes, we identified Artn as a keratinocyte-specific AhR target gene whose product (the neurotrophic factor artemin) was responsible for epidermal hyper-innervation that led to hypersensitivity to pruritus. The activation of AhR via air pollutants induced expression of artemin, alloknesis, epidermal hyper-innervation and inflammation. AhR activation and ARTN expression were positively correlated in the epidermis of patients with atopic dermatitis. Thus, AhR in keratinocytes senses environmental stimuli and elicits an atopic-dermatitis pathology. We propose a mechanism of air-pollution-induced atopic dermatitis via activation of AhR.
[Mh] Termos MeSH primário: Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Dermatite Atópica/imunologia
Epiderme/inervação
Queratina-15/metabolismo
Queratinócitos/fisiologia
Proteínas do Tecido Nervoso/metabolismo
Prurido/imunologia
Receptores de Hidrocarboneto Arílico/metabolismo
[Mh] Termos MeSH secundário: Poluentes Atmosféricos/efeitos adversos
Animais
Animais Recém-Nascidos
Orientação de Axônios/genética
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Células Cultivadas
Epiderme/patologia
Regulação da Expressão Gênica
Seres Humanos
Queratina-15/genética
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Proteínas do Tecido Nervoso/genética
Receptor EphB2/genética
Receptor EphB2/metabolismo
Receptores de Hidrocarboneto Arílico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AHR protein, human); 0 (ARTN protein, human); 0 (Ahr protein, mouse); 0 (Air Pollutants); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Keratin-15); 0 (Krt1-15 protein, mouse); 0 (Nerve Tissue Proteins); 0 (Receptors, Aryl Hydrocarbon); EC 2.7.10.1 (Ephb2 protein, mouse); EC 2.7.10.1 (Receptor, EphB2)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161122
[St] Status:MEDLINE
[do] DOI:10.1038/ni.3614


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[PMID]:28155975
[Au] Autor:Upeniece I; Groma V; Skuja S; Cauce V
[Ad] Endereço:Department of Infectology and Dermatology, Riga Stradins University, Riga, Latvia. dr.ilzeupeniece@gmail.com.
[Ti] Título:Eradication of damaged keratinocytes in cutaneous lichen planus forms demonstrated by evaluation of epidermal and follicular expression of CK15, indices of apoptosis and regulatory protein S100.
[So] Source:Pol J Pathol;67(3):258-269, 2016.
[Is] ISSN:1233-9687
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:The study of cytoskeleton arrangement and its contribution to survival of cell-to-cell contacts appears to be essential for understanding of numerous cellular and tissue processes. Applying CK15, S100 labeling and TUNEL reaction to cutaneous lichen planus subtypes, we found CK15 expression in the outer and inner root sheath of hair follicles, the basal epidermal layer, and eccrine glands. Its follicular expression was decreased in nearby inflammatory infiltrates. The CK15 immunopositivity was mostly described as weak (92.3%) for lichen planus but equally subdivided into weak, moderate and strong in lichen planopilaris (2 = 32.514; df = 4; p < 0.001). The greatly varying apoptotic index was the highest in the lichen planopilaris involving the scalp: 81.2 ±10.7; 87.8 ±10.7 and 88.0 ±10.5 for the basal, spinous and upper epidermal layers, respectively. S100 positive epidermal and follicular cells did not differ in the lesions demonstrated in the study groups; still immunoreactivity was more pronounced in the scalp region of lichen planopilaris. Damage of cell-to-cell contacts was confirmed by electron microscopy. Apart from immunocyte-mediated keratinocyte death, cytoskeleton-based injury and loss of cell-to-cell and matrix contacts may be of great importance, leading to eradication of degrading cells and thus contributing to the pathogenesis of lichen planus.
[Mh] Termos MeSH primário: Queratinócitos/patologia
Líquen Plano/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Apoptose/fisiologia
Feminino
Seres Humanos
Imuno-Histoquímica
Marcação In Situ das Extremidades Cortadas
Queratina-15/análise
Queratina-15/biossíntese
Masculino
Microscopia Eletrônica de Transmissão
Meia-Idade
Proteínas S100/análise
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (KRT15 protein, human); 0 (Keratin-15); 0 (S100 Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE


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[PMID]:27797221
[Au] Autor:Shen YH; Xu CP; Shi ZM; Zhang YJ; Qiao YG; Zhao HP
[Ad] Endereço:Shanxi Medical University in China, Taiyuan, China E-mail : cuipingxu@hotmail.com.
[Ti] Título:Cytokeratin 15 is an Effective Indicator for Progression and Malignancy of Esophageal Squamous Cell Carcinomas.
[So] Source:Asian Pac J Cancer Prev;17(9):4217-4222, 2016.
[Is] ISSN:2476-762X
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To compare the expression level of CK 15 in normal esophageal and esophageal squamous-cell carcinoma (ESCC) tissues and analyse possible functions of CK15 in occurrence and development. MATERIALS AND METHODS: Immunohistochemistry was used to compare CK14, CK15 and proliferating cell nuclear antigen (PCNA) expression levels in ESCCs . Expression level of CK15 was also assessed by Western blotting. In addition, levels of CK15, cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) and PCNA were detected in serum by enzyme-linked immunosorbent assay (ELISA) and chemiluminescence methods. Relationships between clinicopathological parameters and CK14 and CK15 expression were then analyzed. RESULTS: According to immunohistochemistry, in esophageal and intraepithelial neoplasia (SIN) tissues, the expression of CK14, CK15 and PCNA localized to basal layer of the epithelium. CK14 and CK15 levels were higher in normal esophageal squamous epithelial tissue than in SIN and ESCC, and greater in highly differentiated than poorly differentiated carcinoma tissue. By Western blotting, we found more pronounced expression of CK15 in normal esophageal tissue, compared with carcinoma tissue. The specificity of changed CK15 and CYFRA21-1 expression was respectively 90.0% and 96.7% in serum of ESCC patients. Joint detection could improve the sensitivity of esophageal carcinoma diagnosis. Relationships between CK14, CK15 expression and clinical parameters were not statistically significant (P>0.05). Postoperative survival in patients of CK14, CK15 positive expression was longer than with negative expression (χ²=4.352, P=0.037; χ²=9.852, P=0.002). CONCLUSIONS: CK15 expression decreased in esophageal squamous cell carcinoma tissue and serum of esophageal squamous carcinoma patients. We infer that CK15 may play an important role for the occurrence and development of esophageal squamous-cell carcinoma. In the future, CK15 may be used for the diagnosis, treatment and prognostic evaluation of esophageal squamous-cell carcinoma.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Carcinoma de Células Escamosas/secundário
Neoplasias Esofágicas/patologia
Esôfago/metabolismo
Queratina-15/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Western Blotting
Carcinoma de Células Escamosas/metabolismo
Carcinoma de Células Escamosas/terapia
Terapia Combinada
Ensaio de Imunoadsorção Enzimática
Neoplasias Esofágicas/metabolismo
Neoplasias Esofágicas/terapia
Feminino
Seguimentos
Seres Humanos
Técnicas Imunoenzimáticas
Metástase Linfática
Masculino
Meia-Idade
Invasividade Neoplásica
Estadiamento de Neoplasias
Prognóstico
Taxa de Sobrevida
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Keratin-15)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


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[PMID]:27444072
[Au] Autor:Kolivras A; Thompson N; Thompson C
[Ad] Endereço:Departments of Dermatology and Dermatopathology, Saint-Pierre, Brugmann, and Queen Fabiola Children's University Hospitals, Université Libre de Bruxelles, Brussels, Belgium. Electronic address: kolivras@gmail.com.
[Ti] Título:Loss of cytokeratin-15 (CK15) expression is not specific for lichen planopilaris (LPP).
[So] Source:J Am Acad Dermatol;75(2):428-9, 2016 Aug.
[Is] ISSN:1097-6787
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Folículo Piloso/metabolismo
Queratina-15/metabolismo
Líquen Plano/metabolismo
[Mh] Termos MeSH secundário: Seres Humanos
Células-Tronco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Keratin-15)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE


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[PMID]:27411280
[Au] Autor:Chen L; Xi J; Liu D; Zhang X; Lü Y; Li J; Wang J; Zhou J; Nan X; Yue W; Pei X
[Ti] Título:[CO-TRANSPLANTATION OF MOUSE EPIDERMIS AND DERMIS CELLS IN INDUCING HAIR FOLLICLE REGENERATION].
[So] Source:Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi;30(4):485-90, 2016 Apr.
[Is] ISSN:1002-1892
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To investigate the co-transplantation of C57-green fluorescent protein (GFP) mouse epidermis and dermis cells subcutaneously to induce the hair follicle regeneration. METHODS: C57-GFP mouse epidermis and dermis were harvested for isolation the mouse epidermis and dermis cells. The morphology of epidermis and dermis mixed cells at ratio of 1:1 of adult mouse, dermis cells of adult mouse, cultured 3rd generation dermis cells were observed by fluorescence microscope. Immunocytochemistry staining was used to detect hair follicle stem cells markers in cultured 3rd generation dermis cells from new born C57-GFP mouse. And then the epidermis and dermis mixed cells of adult mouse (group A), dermis cells of adult mouse (group B), cultured 3rd generation dermis cells of new born mouse (group C), and saline (group D) were transplanted subcutaneously into Balb/c nude mice. The skin surface of nude mice were observed at 4, 5, 6 weeks of transplantation and hair follicle formation were detected at 6 weeks by immunohistochemistry staining. RESULTS: The isolated C57-GFP mouse epidermis and dermis cells strongly expressed the GFP under the fluorescence microscope. Immunocytochemistry staining for hair follicle stem cells markers in cultured 3rd generation dermis cells showed strong expression of Vimentin and α-smooth muscle actin, indicating that the cells were dermal sheath cells; some cells expressed CD133, Versican, and cytokeratin 15. After transplanted for 4-6 weeks, the skin became black at the injection site in group A, indicating new hair follicle formation. However, no color change was observed in groups B, C, and D. Immunohistochemical staining showed that new complete hair follicles structures formed in group A. GFP expression could be only observed in the hair follicle dermal sheath and outer root sheath in group B, and it could also be observed in the hair follicle dermal sheath, outer root sheath, dermal papilla cells, and sweat gland in group C. The expression of GFP was negative in group D. CONCLUSION: Co-transplantation of mouse epidermis and dermis cells can induce the hair follicle regeneration by means of interaction of each other. And transplantation of isolated dermis cells or cultured dermis cells individually only partly involved in the hair follicles formation.
[Mh] Termos MeSH primário: Derme
Epiderme
Folículo Piloso/crescimento & desenvolvimento
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Alopecia/cirurgia
Animais
Separação Celular
Transplante de Células/métodos
Células Cultivadas
Derme/citologia
Epiderme/citologia
Proteínas de Fluorescência Verde
Folículo Piloso/anatomia & histologia
Queratina-15
Camundongos
Camundongos Nus
Regeneração
Pele/citologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Keratin-15); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160714
[Lr] Data última revisão:
160714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160715
[St] Status:MEDLINE



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