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[PMID]:29256284
[Au] Autor:Fábián R; Kovács A; Stéger V; Frank K; Egerszegi I; Oláh J; Bodó S
[Ad] Endereço:1 National Agricultural Research and Innovation Centre , Szent-Györgyi Albert u. 4, H-2100 Gödöllo , Hungary.
[Ti] Título:X- and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats.
[So] Source:Acta Vet Hung;65(4):500-504, 2017 12.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.
[Mh] Termos MeSH primário: Amelogenina/metabolismo
Transtornos do Desenvolvimento Sexual/veterinária
Doenças das Cabras/genética
Cromossomo X/genética
Cromossomo Y/genética
[Mh] Termos MeSH secundário: Amelogenina/genética
Animais
DNA/química
DNA/genética
Transtornos do Desenvolvimento Sexual/diagnóstico
Transtornos do Desenvolvimento Sexual/genética
Fezes/química
Feminino
Regulação da Expressão Gênica
Genótipo
Doenças das Cabras/diagnóstico
Cabras
Cabelo/química
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amelogenin); 9007-49-2 (DNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.047


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[PMID]:29205009
[Au] Autor:Huang JP; Yang F; Liu YN; Zou KN; Cao Y; Wu D; Chen RH; Ping Y; Zhou HG
[Ad] Endereço:Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
[Ti] Título:[Research Progress on Gene Alterations of Locus in Gender Identification].
[So] Source:Fa Yi Xue Za Zhi;32(5):371-377, 2016 Oct.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:There are two kinds of gene mutation, including mutation in primer-binding region of gene and micro deletion of Y chromosome encompassing gene, and the latter is more common. The mechanisms of mutation in primer-binding region of gene is nucleotide point mutation and the mechanism of micro deletion of Y chromosome encompassing gene maybe non-allelic homologous recombination or non-homologous end-joining. Among the population worldwide, there is a notably higher frequency of gene mutations in Indian population, Sri Lanka population and Nepalese population which reside within the Indian subcontinent. Though gene mutations have little impact on fertility and phenotype, they might cause incorrect result in gender identification. Using composite-amplification kit which including autosomal STR locus, gene locus and multiple Y-STR locus, could avoid wrong gender identification caused by gene mutation.
[Mh] Termos MeSH primário: Amelogenina/genética
Aberrações Cromossômicas
Cromossomos Humanos Y/genética
[Mh] Termos MeSH secundário: Alelos
Grupo com Ancestrais do Continente Asiático/genética
Seres Humanos
Índia
Masculino
Repetições de Microssatélites
Nepal
Reação em Cadeia da Polimerase
Deleção de Sequência
Sri Lanka
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Amelogenin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.05.013


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[PMID]:29337060
[Au] Autor:Ota MS; Kondo K; Li Y; Iseki S; Yamashita A; Gibson CW; Kondo T
[Ad] Endereço:Laboratory of Anatomy, Physiology and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan; Section of Molecular Craniofacial Embryology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Electro
[Ti] Título:Amelogenin X impacts age-dependent increase of frequency and number in labial incisor grooves in C57BL/6.
[So] Source:Biochem Biophys Res Commun;496(2):324-327, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Labial grooves in maxillary incisors have been reported in several wild-type rodent species. Previous studies have reported age-dependent labial grooves occur in moderate prevalence in C57BL/6 mice; however, very little is known about the occurrence of such grooves. In the present study, we observed age-dependent groove formation in C57BL/6 mice up to 26 months after birth and found that not only the frequency of the appearance of incisor grooves but also the number of grooves increased in an age-dependent manner. We examined the molecular mechanisms of age-dependent groove formation by performing DNA microarray analysis of the incisors of 12-month-old (12M) and 24-month-old (24M) mice. Amelx, encoding the major enamel matrix protein AMELOGENIN, was identified as a 12M-specific gene. Comparing with wild-type mice, the maxillary incisors of Amelx mutants indicated the increase of the frequency and number of labial grooves. These findings suggested that the Amelx gene impacts the age-dependent appearance of the labial incisor groove in C57BL/6 mice.
[Mh] Termos MeSH primário: Envelhecimento/genética
Amelogenina/genética
Esmalte Dentário/metabolismo
Dentina/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Incisivo/metabolismo
[Mh] Termos MeSH secundário: Envelhecimento/metabolismo
Envelhecimento/patologia
Amelogenina/deficiência
Animais
Esmalte Dentário/diagnóstico por imagem
Esmalte Dentário/patologia
Dentina/diagnóstico por imagem
Dentina/patologia
Incisivo/diagnóstico por imagem
Incisivo/patologia
Maxila/diagnóstico por imagem
Maxila/metabolismo
Maxila/patologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Análise de Sequência com Séries de Oligonucleotídeos
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amelogenin); 0 (Amelx protein, mouse)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


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[PMID]:28574578
[Au] Autor:Kim Y; Hur SW; Jeong BC; Oh SH; Hwang YC; Kim SH; Koh JT
[Ad] Endereço:Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, South Korea.
[Ti] Título:The Fam50a positively regulates ameloblast differentiation via interacting with Runx2.
[So] Source:J Cell Physiol;233(2):1512-1522, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Differentiated ameloblasts secret enamel matrix proteins such as amelogenin, ameloblastin, and enamelin. Expression levels of these proteins are regulated by various factors. To find a new regulatory factor for ameloblast differentiation, we performed 2D-PAGE analysis using mouse ameloblast lineage cell line (mALCs) cultured with mineralizing medium. Of identified proteins, family with sequence similarity 50 member A (Fam50a) was significantly increased during differentiation of mALCs. Fam50a protein was also highly expressed in secretory ameloblasts of mouse tooth germs. In mALCs cultures, forced expression of Fam50a up-regulated the expression of enamel matrix protein genes such as amelogenin, ameloblastin, and enamelin. In addition, up-regulation of Fam50a also increased ALP activity and mineralized nodule formation in a dose-dependent manner. In contrast, knockdown of Fam50a decreased expression levels of enamel matrix protein genes, ALP activity, and mineralized nodule formation. By fluorescence microscopy, endogenous Fam50a protein was found to be localized to the nucleus of ameloblasts. In addition, Fam50a synergistically increased Ambn transactivation by Runx2. Moreover, Fam50a increased binding affinity of Runx2 to Ambn promoter by physically interacting with Runx2. Taken together, these results suggest Fam50a might be a new positive regulator of ameloblast differentiation.
[Mh] Termos MeSH primário: Ameloblastos/metabolismo
Diferenciação Celular
Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo
Proteínas de Ligação a DNA/metabolismo
Dente Molar/metabolismo
Proteínas Nucleares/metabolismo
[Mh] Termos MeSH secundário: Fosfatase Alcalina/metabolismo
Amelogenina/genética
Amelogenina/metabolismo
Animais
Sítios de Ligação
Células Cultivadas
Subunidade alfa 1 de Fator de Ligação ao Core/genética
Proteínas de Ligação a DNA/genética
Proteínas do Esmalte Dentário/genética
Proteínas do Esmalte Dentário/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Camundongos Endogâmicos C57BL
Proteínas Nucleares/genética
Regiões Promotoras Genéticas
Transdução de Sinais
Fatores de Tempo
Calcificação de Dente
Transcrição Genética
Ativação Transcricional
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ambn protein, mouse); 0 (Amelogenin); 0 (Core Binding Factor Alpha 1 Subunit); 0 (DNA-Binding Proteins); 0 (Dental Enamel Proteins); 0 (FAM50A protein, human); 0 (Nuclear Proteins); 0 (Runx2 protein, mouse); 0 (tuftelin); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.26038


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[PMID]:28628900
[Au] Autor:Ristow PG; Barnes N; Murphy GP; Brown H; Cloete KW; D'Amato ME
[Ad] Endereço:Forensic DNA Laboratory, Department of Biotechnology, University of the Western Cape, Bellville, 7535, South Africa; Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark.
[Ti] Título:Evaluation of the InnoTyper 21 genotyping kit in multi-ethnic populations.
[So] Source:Forensic Sci Int Genet;30:43-50, 2017 Sep.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We report the findings of the evaluation of the InnoTyper 21 genotyping kit for the use of human identification (HID) and paternity testing in South Africa. This novel forensic kit evaluates 20 retrotransposable elements (AC4027, MLS26, ALU79712, NBC216, NBC106, RG148, NBC13, AC2265, MLS09, AC1141, TARBP, AC2305, HS4.69, NBC51, ACA1766, NBC120, NBC10, NBC102, SB19.12 and NBC148) and the Amelogenin locus for sex determination. The evaluation of the genotyping performance showed no significant spectral pull-up for peak heights between 100 and 30,000 RFUs. All loci presented biallelic patterns except the triallelic RG148 locus resulting from a variant insertion allele, named RG148I-1, observed exclusively in the Bantu. The InnoTyper 21 kit was found to be highly discriminatory between the 507 unrelated individuals of the Afrikaaner, Asian Indian, Coloured, amaXhosa and amaZulu groups. The HID parameters: the CPD ranged between 0.99999987 and 0.9999999845, and the CMP between 1.0335×10 and 1.5506×10 . The paternity parameters: the CPI ranged between 0.0202 and 0.3177, and the CPE between 0.9161 and 0.9749. There were no significant signs of deviations from HWE or linkage disequilibrium (LD) after applying a Bonferroni correction. This kit also showed minor levels of population structure which could differentiate between the African and non-African population groups. Finally, in challenging casework with severely degraded biological material, the InnoTyper 21 genotyping kit was compatible with GlobalFiler and Investigator DIPplex to increase the HID parameters.
[Mh] Termos MeSH primário: Impressões Digitais de DNA/instrumentação
Grupos Étnicos/genética
Genética Populacional
Genótipo
[Mh] Termos MeSH secundário: Alelos
Amelogenina/genética
Análise Discriminante
Seres Humanos
Repetições de Microssatélites
Análise para Determinação do Sexo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amelogenin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


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[PMID]:28511096
[Au] Autor:Berlyne S; Oz C; Einot N; Avraham S; Ram T; Goldberg MD; Gafny R
[Ad] Endereço:DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q., Jerusalem, Israel.
[Ti] Título:Improved amplification results following episodes of failure to amplify at the Amelogenin Locus using PowerPlex ESI 16 Fast System.
[So] Source:Forensic Sci Int Genet;29:257-260, 2017 Jul.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In 2012 the Israel Police DNA Casework laboratory adopted the 16 STR PowerPlex ESI kit for routine use. The Promega Company updated this kit and developed the PowerPlex ESI 16 Fast System in which all autosomal primer pairs remained identical to the original set, except at the amelogenin site. The master mix was improved and optimized which allowed for direct, faster and more robust amplification. Prior to implementing the PowerPlex ESI 16 Fast System in our lab, we conducted a preliminary assay where 213 casework samples were amplified using the new kit. These samples had previously been extracted by one of two extraction kits employed by our lab. (the PrepFiler ExpressTM and PrepFiler BTATM Forensic DNA Extraction Kits). The amplification results from these samples were compared to samples amplified using the original PowerPlex ESI 16 kit. Multiple incidents of failure to amplify at the amelogenin locus were noted using the new system with the recommended protocol at a rate of 13% (28 of 213 samples). Experiments were performed to understand whether these amplification failures could be a result of primer binding site mutations, extraction method reagents and/or inhibitors. The conclusions reached following these experiments, in conjunction with consultation with the manufacturer, led to the trial of a modified amplification protocol where the suggested annealing temperature was reduced by 2 degrees. To evaluate the efficiency of this altered protocol, a comparison study was undertaken where 88 additional casework samples were chosen and amplified using both the modified 58°C and the recommended 60°C annealing temperatures. We concluded that the most effective method in our laboratory for achieving a consistent and balanced amplification at the amelogenin locus was to reduce the annealing temperature from the manufacturer's recommended 60°C to 58°C. This modification resulted in a reduction of the failure to amplify at the amelogenin locus from 13% (28/213) to 1.1% (1/88) without any observed changes to the autosomal STR amplification results.
[Mh] Termos MeSH primário: Amelogenina/genética
Impressões Digitais de DNA
Repetições de Microssatélites
Reação em Cadeia da Polimerase Multiplex/instrumentação
Reação em Cadeia da Polimerase Multiplex/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amelogenin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE


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[PMID]:28391141
[Au] Autor:Brown H; Thompson R; Murphy G; Peters D; La Rue B; King J; Montgomery AH; Carroll M; Baus J; Sinha S; Wendt FR; Song B; Chakraborty R; Budowle B; Sinha SK
[Ad] Endereço:InnoGenomics Technologies, LLC, 1441 Canal Street, Suite 307, New Orleans, LA 70112, USA. Electronic address: hbrown@innogenomics.com.
[Ti] Título:Development and validation of a novel multiplexed DNA analysis system, InnoTyper 21.
[So] Source:Forensic Sci Int Genet;29:80-99, 2017 Jul.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We report here a novel multiplexed DNA analysis system consisting of 20 Alu markers and Amelogenin for analysis of highly degraded forensic biological samples. The key to the success of the system in obtaining results from degraded samples is the primer design yielding small amplicon size (60-125bp) for all 20 markers. The markers included in the InnoTyper 21 system are bi-allelic, having two possible allelic states (insertion or null) and thus termed INNULs. The markers are short interspersed nuclear elements (SINEs), a category of retrotransposable elements (REs) which are non-coding genomic DNA repeat sequences, or "mobile insertion elements," comprising approximately 40% of the human genome. Alu elements are primate specific SINEs that have reached a copy number in excess of one million in the human genome, which makes these markers highly sensitive and desirable for forensic samples with extremely degraded DNA. Until now however, due to the inherent size difference associated with insertion and no insertion alleles, the use of Alu REs has not been practical for forensic applications. The novel primer design described herein has allowed the development of a multiplexed Alu system yielding fragment sizes amenable to degraded DNA samples, as frequently encountered in missing persons cases or forensic samples such as hair shafts. Although use of Alus in human identity has been studied using single marker amplification and reported before, we report for the first time development and validation of a system with multiplexed RE markers. Studies performed include PCR optimization, species specificity, sensitivity, degradation and inhibition, precision and accuracy, nonprobative samples, mixture, and population database studies. A population study using 592 samples including five populations was performed using InnoTyper 21. The data indicated the random match probability for the combination of these 20 Alu markers was greater than 1 in 3.8 million for the populations studied, indicating the greater statistical power of these autosomal nuclear DNA markers over haplotype systems typically used in such degraded samples. Results demonstrate the system is successful in obtaining results from highly degraded DNA. A sensitivity study performed demonstrated at least 95% recovery of alleles from as low as 50pg of total input DNA, and partial profiles from as low as 25pg. This study has demonstrated that the bi-allelic INNULs in the InnoTyper 21 system provide a sensitivity of detection and a power of discrimination that makes them useful for human identification of extremely degraded samples.
[Mh] Termos MeSH primário: Impressões Digitais de DNA/instrumentação
[Mh] Termos MeSH secundário: Alelos
Elementos Alu/genética
Amelogenina/genética
Animais
Grupos de Populações Continentais/genética
Degradação Necrótica do DNA
Eletroforese Capilar
Marcadores Genéticos
Variação Genética
Seres Humanos
Reação em Cadeia da Polimerase
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Amelogenin); 0 (Genetic Markers)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170410
[St] Status:MEDLINE


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[PMID]:28363008
[Au] Autor:Gao HM; Wang C; Han SY; Sun SH; Xiao DJ; Wang YS; Li CT; Zhang MX
[Ad] Endereço:Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, China.
[Ti] Título:Analysis of the 19 Y-STR and 16 X-STR loci system in the Han population of Shandong province, China.
[So] Source:Genet Mol Res;16(1), 2017 Mar 30.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The sex-linked short tandem repeats (STR), Y-STR and X-STR, are important for autosomal STRs in forensic paternity testing. We evaluated the forensic parameters of 19 Y-STRs and 16 X-STRs in the Han population of Shandong province, China. A Goldeneye 20Y kit (DYS391, DYS389I, DYS390, DYS389II, DYS348, DYS456, Y-GATA-H4, DYS447, DYS19, DYS392, DYS393, DYS388, DYS439, DYS635, DYS448, DYS460, DYS458, DYS437, DYS385 a/b) was used to analyze the forensic parameters of 534 unrelated males. A Goldeneye17X system (DXS6795, DXS9902, DXS8378, HPRTB, GATA165B12, DXS7132, DXS7424, DXS6807, DXS6803, GATA172D05, DXS6800, DXS10134, GATA31E08, DXS10159, DXS6789, DXS6810, amelogenin) was used to analyze 97 unrelated males and 214 females. In addition, we used the kits to examine 5 cases with abnormal amelogenin test results, as well as a male child with agenosomia typed by autosomal STR. We found 203 Y-STR haplotypes with allele frequencies ranging from 0.0019 to 0.7959, and GD ranging from 0.3429 to 0.9667. Expect in DXS6803, the allele frequencies of the other 15 X-STR loci showed no differences between females and males. PD ranged from 0.5504 to 0.9638, while PD ranged from 0.3176 to 0.8377. With the exception of DXS6803 and DXS6810, the allele frequencies of other X-STR loci were in accordance with Hardy-Weinberg equilibrium in females. One amelogenin negative case was characterized as a deletion of Y-DYS458. This paper provided data regarding the genetic polymorphism of Y-STRs and X-STRs in the Han population, and demonstrated the importance of Y-STR and X-STR in forensic autosomal STR analysis.
[Mh] Termos MeSH primário: Cromossomos Humanos X
Cromossomos Humanos Y
Repetições de Microssatélites
[Mh] Termos MeSH secundário: Alelos
Amelogenina/genética
Grupo com Ancestrais do Continente Asiático/genética
China
Impressões Digitais de DNA
Grupos Étnicos/genética
Feminino
Genética Forense/métodos
Frequência do Gene/genética
Genética Populacional
Haplótipos
Seres Humanos
Masculino
Paternidade
Polimorfismo Genético
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amelogenin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16019573


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[PMID]:28326729
[Au] Autor:Kun T; Xiaoyun F; Qin D; Chuhang L; Xiaohua R
[Ad] Endereço:Dept. of Stomatology, Hospital of University of Electronic Science and Technology of China UESTC & Sichuan Provincial People's Hosptial, Chengdu 610072, China.
[Ti] Título:[Study of human leucine-rich amelogenin peptide and its regulation of mineralization by cryogenic transmission electron microscopy].
[So] Source:Hua Xi Kou Qiang Yi Xue Za Zhi;35(1):63-67, 2017 Feb 01.
[Is] ISSN:1000-1182
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: Recombinant human leucine-rich amelogenin peptide (LRAP) was studied by cryogenic transmission electron microscopy (TEM); evaluation focused on its self-assembly and crystal growth in vitro. METHODS: Human LRAP was recombined through prokaryotic expression vector pCold-SUMO and transformed into Escherichia coli BL21plys to acquire purified proteins. Cryogen TEM recorded assembly and self-assembling of LRAP from pH 3.5 to pH 8.0, and the hydroxyapatite crystal growth in the mixture of LRAP protein solution and artificial saliva was observed using TEM and selected area electron diffraction. RESULTS: More than 90% purity LRAP was expressed, purified and identified as described in methods. LRAP linked into oligomers, nanospheres, nanochains, and microribbons, whereas pH value increased from 3.5 to 8.0. Mature hydroxyapatite crystal growth was guided in artificial saliva filled with calcium phosphate. CONCLUSIONS: LRAP is simplified amelogenin functional domain and conserved the basic characters of amelogenin such as self-assembling and inducing crystallization along c axis. In the area of acellular synthesis of hydroxyapatite using extracellular enamel matrix protein, LRAP is one of candidate repair materials for irregular hard tissue defection.
.
[Mh] Termos MeSH primário: Densidade Óssea
[Mh] Termos MeSH secundário: Amelogenina
Fosfatos de Cálcio
Cristalização
Esmalte Dentário
Proteínas do Esmalte Dentário
Durapatita
Seres Humanos
Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amelogenin); 0 (Calcium Phosphates); 0 (Dental Enamel Proteins); 0 (enamel matrix proteins); 0 (leucine-rich amelogenin peptide); 91D9GV0Z28 (Durapatite); 97Z1WI3NDX (calcium phosphate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170412
[Lr] Data última revisão:
170412
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.7518/hxkq.2017.01.009


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[PMID]:28189975
[Au] Autor:Han J; Sun J; Zhao L; Zhao W; Liu Y; Li C
[Ad] Endereço:Key Laboratory of Forensic Genetics, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China; Technology Department of Chaoyang Sub-Bureau, Beijing Public Security Bureau, Beijing, 100102, China.
[Ti] Título:Validation study of a 15-plex rapid STR amplification system for human identification.
[So] Source:Forensic Sci Int Genet;28:71-81, 2017 May.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Conventional PCR amplification requires approximately 3h to complete and thus does not meet the requirements of rapid DNA analysis. The purpose of this study was to validate a rapid 15-plex PCR system that can amplify 14 autosomal short tandem repeat (STR) loci (i.e., D6S1043, D21S11, D7S820, CFS1PO, D2S1338, D3S1358, D13S317, D8S1179, D16S539, Penta E, D5S818, vWA, D18S51, and FGA) and Amelogenin. This system was validated by sensitivity, species specificity, inhibitor tests, sizing accuracy, stutter calculation, concordance tests, DNA mixture, and case sample tests according to the Scientific Working Group for DNA Analysis Methods (SWGDAM) guidelines and Chinese criteria. We found that the rapid 15-plex PCR system could shorten the amplification time to 37min and proved that it provides an alternative method for conventional PCR in human identification detection.
[Mh] Termos MeSH primário: Impressões Digitais de DNA/métodos
Repetições de Microssatélites
Reação em Cadeia da Polimerase Multiplex/instrumentação
[Mh] Termos MeSH secundário: Amelogenina/genética
Animais
Genética Forense
Seres Humanos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Amelogenin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170213
[St] Status:MEDLINE



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