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[PMID]:28453109
[Au] Autor:Tabima-Cubillos LY; Chaparro-Giraldo A; Trujillo-Güiza ML
[Ad] Endereço:Universidad Nacional de Colombia, Bogotá, lytabimac@unal.edu.co.
[Ti] Título:[Detection of transgenic proteins in maize flour marketed in Bogotá, Colombia].
[Ti] Título:Detección de proteínas transgénicas en harinas de maíz comercializadas en Bogotá, Colombia..
[So] Source:Rev Salud Publica (Bogota);18(3):470-483, 2016 Jun.
[Is] ISSN:0124-0064
[Cp] País de publicação:Colombia
[La] Idioma:spa
[Ab] Resumo:Objective To detect the presence or absence of transgenic proteins derived from GM crops in maize flour marketed in Bogota D.C., Colombia. Methods 11 extraction protocols for total protein were evaluated in 17 precooked flour, two uncooked and three positive controls. Subsequently, the presence of 7 transgenic proteins (CP4-EPSPS, Cry1Ab, Cry1Ac, Cry1F, Cry2A, Cry34Ab1 and Cry3Bb1) using commercial ELISA kits was determined. Results It was determined that the best protocol for total protein extraction was buffer with Triton X-100, which allowed obtaining protein concentrations greater than 0.5 mg per gram of flour and does not generate interference with the ELISA technique. Four transgenic proteins were detected: CP4EPSPS, Cry1F, Cry1Ab and Cry34Ab1 in precooked and uncooked flour with percentages varying between 20 and 100 %. Conclusion Seven of the 19 maize flours contain traces of transgenic protein (B2,B8,A3,O3,O1,C1 and C2) that provide resistance to lepidopterans and coleopterans, and tolerance to glyphosate herbicide, (CP4EPSPS- Cry1Ab, Cry1F, Cry34Ab1 and Cry3Bb1). All detected events are approved for human consumption in Colombia, according to the Ministry of Health and Social Protection.
[Mh] Termos MeSH primário: Farinha/análise
Plantas Geneticamente Modificadas/genética
Proteínas de Vegetais Comestíveis/genética
Zea mays/genética
[Mh] Termos MeSH secundário: Colômbia
Ensaio de Imunoadsorção Enzimática
Proteínas de Vegetais Comestíveis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vegetable Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  2 / 1616 MEDLINE  
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[PMID]:29045496
[Au] Autor:Revel A; Jarzaguet M; Peyron MA; Papet I; Hafnaoui N; Migné C; Mosoni L; Polakof S; Savary-Auzeloux I; Rémond D; Dardevet D
[Ad] Endereço:Université Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, PFEM, MetaboHUB-Clermont, CRNH Auvergne, Clermont-Ferrand, France.
[Ti] Título:At same leucine intake, a whey/plant protein blend is not as effective as whey to initiate a transient post prandial muscle anabolic response during a catabolic state in mini pigs.
[So] Source:PLoS One;12(10):e0186204, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Muscle atrophy has been explained by an anabolic resistance following food intake and an increase of dietary protein intake is recommended. To be optimal, a dietary protein has to be effective not only to initiate but also to prolong a muscle anabolic response in a catabolic state. To our knowledge, whether or not a dairy or a dairy/plant protein blend fulfills these criterions is unknown in a muscle wasting situation. OBJECTIVE: Our aim was, in a control and a catabolic state, to measure continuously muscle anabolism in term of intensity and duration in response to a meal containing casein (CAS), whey (WHEY) or a whey/ plant protein blend (BLEND) and to evaluate the best protein source to elicit the best post prandial anabolism according to the physio-pathological state. METHODS: Adult male Yucatan mini pigs were infused with U-13C-Phenylalanine and fed either CAS, WHEY or BLEND. A catabolic state was induced by a glucocorticoid treatment for 8 days (DEX). Muscle protein synthesis, proteolysis and balance were measured with the hind limb arterio-venous differences technique. Repeated time variance analysis were used to assess significant differences. RESULTS: In a catabolic situation, whey proteins were able to initiate muscle anabolism which remained transient in contrast to the stimulated muscle protein accretion with WHEY, CAS or BLEND in healthy conditions. Despite the same leucine intake compared to WHEY, BLEND did not restore a positive protein balance in DEX animals. CONCLUSIONS: Even with WHEY, the duration of the anabolic response was not optimal and has to be improved in a catabolic state. The use of BLEND remained of lower efficiency even at same leucine intake than whey.
[Mh] Termos MeSH primário: Anabolizantes/administração & dosagem
Caseínas/administração & dosagem
Leucina/metabolismo
Atrofia Muscular/dietoterapia
Proteínas de Vegetais Comestíveis/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Ingestão de Alimentos
Glucocorticoides/administração & dosagem
Metabolismo/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/metabolismo
Atrofia Muscular/metabolismo
Atrofia Muscular/patologia
Período Pós-Prandial/efeitos dos fármacos
Suínos
Porco Miniatura
Soro do Leite/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anabolic Agents); 0 (Caseins); 0 (Glucocorticoids); 0 (Vegetable Proteins); GMW67QNF9C (Leucine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171019
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186204


  3 / 1616 MEDLINE  
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[PMID]:28723948
[Au] Autor:Abernathy J; Brezas A; Snekvik KR; Hardy RW; Overturf K
[Ad] Endereço:Hagerman Fish Culture Experiment Station, USDA-ARS, Hagerman, Idaho, United States of America.
[Ti] Título:Integrative functional analyses using rainbow trout selected for tolerance to plant diets reveal nutrigenomic signatures for soy utilization without the concurrence of enteritis.
[So] Source:PLoS One;12(7):e0180972, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Finding suitable alternative protein sources for diets of carnivorous fish species remains a major concern for sustainable aquaculture. Through genetic selection, we created a strain of rainbow trout that outperforms parental lines in utilizing an all-plant protein diet and does not develop enteritis in the distal intestine, as is typical with salmonids on long-term plant protein-based feeds. By incorporating this strain into functional analyses, we set out to determine which genes are critical to plant protein utilization in the absence of gut inflammation. After a 12-week feeding trial with our selected strain and a control trout strain fed either a fishmeal-based diet or an all-plant protein diet, high-throughput RNA sequencing was completed on both liver and muscle tissues. Differential gene expression analyses, weighted correlation network analyses and further functional characterization were performed. A strain-by-diet design revealed differential expression ranging from a few dozen to over one thousand genes among the various comparisons and tissues. Major gene ontology groups identified between comparisons included those encompassing central, intermediary and foreign molecule metabolism, associated biosynthetic pathways as well as immunity. A systems approach indicated that genes involved in purine metabolism were highly perturbed. Systems analysis among the tissues tested further suggests the interplay between selection for growth, dietary utilization and protein tolerance may also have implications for nonspecific immunity. By combining data from differential gene expression and co-expression networks using selected trout, along with ontology and pathway analyses, a set of 63 candidate genes for plant diet tolerance was found. Risk loci in human inflammatory bowel diseases were also found in our datasets, indicating rainbow trout selected for plant-diet tolerance may have added utility as a potential biomedical model.
[Mh] Termos MeSH primário: Ração Animal/análise
Fenômenos Fisiológicos da Nutrição Animal
Aquicultura/métodos
Dieta
Enterite/etiologia
Oncorhynchus mykiss/fisiologia
Proteínas de Vegetais Comestíveis/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Carnivoridade
Sequenciamento de Nucleotídeos em Larga Escala
Nutrigenômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vegetable Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180972


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[PMID]:28687361
[Au] Autor:Eya JC; Yossa R; Perera D; Okubajo O; Gannam A
[Ad] Endereço:Department of Biology/Gus R. Douglass Institute, West Virginia State University, Institute, WV 25112, USA. Electronic address: eyajc@wvstateu.edu.
[Ti] Título:Combined effects of diets and temperature on mitochondrial function, growth and nutrient efficiency in rainbow trout (Oncorhynchus mykiss).
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;212:1-11, 2017 Oct.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A 4×3 factorial experiment was conducted to evaluate the effects of two dietary protein sources (mixed fishmeal/plant protein-, and plant protein- based diet), two dietary lipid levels (10% and 20%) and three water temperatures (10°C, 14°C, and 18°C) on the growth performance, nutrient utilization efficiencies and mitochondrial enzyme complex activities in rainbow trout Oncorhynchus mykiss (average weight±SD, 39.5±5g) over a 180day rearing period. At the end of the experiment, weight gain (WG), condition factor (CF), and feed efficiency (FE) were significantly affected by diet×temperature interaction (P<0.05). Specific growth rate (SGR) was significantly affected by increasing temperature (P<0.05). The plant protein-based diets led to a higher CF than the mixed fishmeal/plant protein-based diets. The protein productive value (PPV), protein efficiency ratio (PER), lipid efficiency ratio, (LER) and lipid productive value (LPV) were all significantly affected by diet×temperature interaction (P<0.05). The diet×temperature interaction also had significant effects on mitochondrial enzyme complexes II, V and citrate synthase in the liver, complexes II and IV in the intestine, and complex IV in the muscle (P<0.05). Temperature had a significant main effect on the activity of the enzymatic complexes I and III in the liver, complex III and citrate synthase in the intestine, and complexes I, II, III, V and citrate synthase in the muscle (P<0.05). Diet had a significant main effect on complexes I and III in the liver, complexes II and III for the intestine and complexes I and II in the muscle (P<0.05). The significant temperature x diet interaction observed has practical ecological implications explicitly demonstrating how changes in temperature regimens as anticipated in the rising global temperature can influence organismal performance in relation to changes in dietary formulations (replacing fishmeal based diet with plant protein based ingredients). To illustrate the practical application of the observations from this study, the most economical and cost effective way to produce rainbow trout would be to use 40/10PP diet at 14°C because fish fed this treatment had a weight gain comparable to that of the fish fed the more expensive experimental diets (40/10 FM/PP, 40/20 FM/PP, and 40/20 PP).
[Mh] Termos MeSH primário: Dieta
Oncorhynchus mykiss/metabolismo
[Mh] Termos MeSH secundário: Ração Animal/análise
Fenômenos Fisiológicos da Nutrição Animal
Animais
Proteínas na Dieta/administração & dosagem
Complexo de Proteínas da Cadeia de Transporte de Elétrons/metabolismo
Proteínas de Peixes/administração & dosagem
Aquecimento Global
Metabolismo dos Lipídeos
Mitocôndrias/metabolismo
Oncorhynchus mykiss/crescimento & desenvolvimento
Temperatura Ambiente
Proteínas de Vegetais Comestíveis/administração & dosagem
Ganho de Peso
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Proteins); 0 (Electron Transport Chain Complex Proteins); 0 (Fish Proteins); 0 (Vegetable Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE


  5 / 1616 MEDLINE  
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[PMID]:28487340
[Au] Autor:Ozawa M; Yoshida D; Hata J; Ohara T; Mukai N; Shibata M; Uchida K; Nagata M; Kitazono T; Kiyohara Y; Ninomiya T
[Ad] Endereço:From the Department of Epidemiology and Public Health (M.O., D.Y., J.H., T.O., N.M., M.S., T.N.), Center for Cohort Studies (D.Y., J.H., N.M., M.S., T.N.), Department of Medicine and Clinical Science (J.H., N.M., T.K.), and Department of Neuropsychiatry (T.O.), Graduate School of Medical Sciences, K
[Ti] Título:Dietary Protein Intake and Stroke Risk in a General Japanese Population: The Hisayama Study.
[So] Source:Stroke;48(6):1478-1486, 2017 Jun.
[Is] ISSN:1524-4628
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND PURPOSE: The influence of dietary protein intake on stroke risk is an area of interest. We investigated the association between dietary protein intake and stroke risk in Japanese, considering sources of protein. METHODS: A total of 2400 subjects aged 40 to 79 years were followed up for 19 years. Dietary protein intake was estimated using a 70-item semiquantitative food frequency questionnaire. The risk estimates for incident stroke and its subtypes were calculated using a Cox proportional hazards model. RESULTS: During the follow-up, 254 participants experienced stroke events; of these, 172 had ischemic stroke, and 58 had intracerebral hemorrhage. Higher total protein intake was significantly associated with lower risks of stroke and intracerebral hemorrhage (both for trend <0.05). With regard to sources of protein, the risks of total stroke and ischemic stroke significantly decreased by 40% (95% confidence interval, 12%-59%) and 40% (5%-62%), respectively, in subjects with the highest quartile of vegetable protein intake compared with those with the lowest one. In contrast, subjects with the highest quartile of animal protein intake had a 53% (4%-77%) lower risk of intracerebral hemorrhage. Vegetable protein intake was positively correlated with intakes of soybean products, vegetable, and algae, whereas animal protein intake was positively correlated with intakes of fish, meat, eggs, and milk/dairy products. Both types of protein intakes were negatively correlated with intakes of rice and alcohol. CONCLUSIONS: Our findings suggest that higher dietary protein intake is associated with a reduced risk of stroke in the general Japanese population.
[Mh] Termos MeSH primário: Isquemia Encefálica/epidemiologia
Hemorragia Cerebral/epidemiologia
Dieta/estatística & dados numéricos
Proteínas Dietéticas do Ovo
Carne/estatística & dados numéricos
Proteínas do Leite
Acidente Vascular Cerebral/epidemiologia
Proteínas de Vegetais Comestíveis
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Seguimentos
Seres Humanos
Japão/epidemiologia
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Egg Proteins, Dietary); 0 (Milk Proteins); 0 (Vegetable Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1161/STROKEAHA.116.016059


  6 / 1616 MEDLINE  
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[PMID]:28317712
[Au] Autor:Babini E; Tagliazucchi D; Martini S; Dei Più L; Gianotti A
[Ad] Endereço:Department of Agri-Food Sciences and Technologies, Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy. Electronic address: elena.babini2@unibo.it.
[Ti] Título:LC-ESI-QTOF-MS identification of novel antioxidant peptides obtained by enzymatic and microbial hydrolysis of vegetable proteins.
[So] Source:Food Chem;228:186-196, 2017 Aug 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bioactive antioxidant peptides are more and more attracting the attention of food manufacturers for their potential to transform food in functional food, able to prevent a variety of chronic diseases associated with oxidative stress. In the present study proteins extracted from different vegetable sources (KAMUT® khorasan wheat, emmer, lupine and pea) were hydrolyzed with commercial enzymes and Lactobacillus spp. strains. Hydrolysates were separated by size exclusion chromatography and purified fractions were analyzed for their antioxidant activity. Peptides from the fractions with the highest activity were identified by nanoLC-ESI-QTOF-MS and thirteen peptides were selected for synthesis on the basis of their sequence. Four peptides (VLPPQQQY, TVTSLDLPVLRW, VTSLDLPVLRW, FVPY) were found able to scavenge superoxide anion and hydroxyl radicals, organic nitro-radicals (ABTS, DPPH) and to inhibit lipid peroxidation. The impact of this work is targeted to add hydrolysed vegetable proteins to reformulated functional food or to produce health-promoting ingredients and nutraceuticals.
[Mh] Termos MeSH primário: Antioxidantes/química
Cromatografia Líquida/métodos
Espectrometria de Massas/métodos
Peptídeos/química
Proteínas de Vegetais Comestíveis/química
[Mh] Termos MeSH secundário: Hidrólise
Peroxidação de Lipídeos
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Peptides); 0 (Vegetable Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE


  7 / 1616 MEDLINE  
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[PMID]:28302185
[Au] Autor:Grosse Brinkhaus A; Wyss U; Arrigo Y; Girard M; Bee G; Zeitz JO; Kreuzer M; Dohme-Meier F
[Ad] Endereço:1Agroscope, Institute for Livestock Sciences (ILS),Tioleyre 4,1725 Posieux,Switzerland.
[Ti] Título:In vitro ruminal fermentation characteristics and utilisable CP supply of sainfoin and birdsfoot trefoil silages and their mixtures with other legumes.
[So] Source:Animal;11(4):580-590, 2017 Apr.
[Is] ISSN:1751-732X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The extensive protein degradation occurring during ensiling decreases the nutritive value of silages, but this might be counteracted by tannins. Therefore, silages from two legume species containing condensed tannins (CT) - sainfoin (SF) and birdsfoot trefoil (two cultivars: birdsfoot trefoil, cv. Bull (BTB) and birdsfoot trefoil, cv. Polom) - were compared for their in vitro ruminal fermentation characteristics. The effect of combining them with two CT-free legume silages (lucerne (LU) and red clover (RC)) was also determined. The supply of duodenally utilisable CP (uCP) in the forages was emphasised. The legumes were each harvested from three field sites. After 24 h of wilting on the field, the legumes were ensiled in laboratory silos for 86 days. Proximate constituents, silage fermentation characteristics, CT content and CP fractions were determined. Subsequently, silage samples and 1 : 1 mixtures of the CT-containing and CT-free silages were incubated for 24 h in batch cultures using ruminal fluid and buffer (1 : 2, v/v). Each treatment was replicated six times in six runs. The effects on pH, ammonia and volatile fatty acid concentrations, protozoal counts, and total gas and methane production were determined. uCP content was calculated by considering the CP in the silage and the ammonia in the incubation fluid from treatments and blanks. Statistical evaluation compared data from single plants alone and together with that from the mixtures. Among treatments, SF silage contained the least CP and the most CT. The non-protein nitrogen content was lower, favouring neutral detergent soluble and insoluble protein fractions, in the SF and RC silages. Absolute uCP content was lowest in SF and SF mixtures, although the ratio to total CP was the highest. In comparison with LU, the ammonia concentration of the incubation fluid was lower for SF, RC and BTB and for the mixture of SF with LU. The total gas and methane production was similar among the treatments, and the total volatile fatty acid production was decreased with the CT-containing legumes. Protozoal count was increased with the mixtures containing LU and either SF or BTB compared with single LU. In conclusion, compared with the other legumes, SF and RC have similar advantages as they show limited proteolysis during ensiling. In addition, SF supplies more uCP relative to total CP. The CT-containing legumes also differed in their effect on ruminal fermentation and ammonia formation, probably because of their different CT contents. Thus, SF and its mixtures appear promising for improving the protein utilisation of ruminants.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos da Nutrição Animal
Bovinos/fisiologia
Fabaceae/química
Silagem/análise
Proteínas de Vegetais Comestíveis/metabolismo
[Mh] Termos MeSH secundário: Animais
Dieta/veterinária
Feminino
Fermentação
Loteae/química
Proantocianidinas/análise
Rúmen/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proanthocyanidins); 0 (Vegetable Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1017/S1751731116001816


  8 / 1616 MEDLINE  
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[PMID]:28242361
[Au] Autor:Caballero-Solares A; Hall JR; Xue X; Eslamloo K; Taylor RG; Parrish CC; Rise ML
[Ad] Endereço:Department of Ocean Sciences, Memorial University of Newfoundland, 1 Marine Lab Road, St. John's, NL A1C 5S7, Canada. Electronic address: acaballeroso@mun.ca.
[Ti] Título:The dietary replacement of marine ingredients by terrestrial animal and plant alternatives modulates the antiviral immune response of Atlantic salmon (Salmo salar).
[So] Source:Fish Shellfish Immunol;64:24-38, 2017 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effects of replacing marine ingredients by terrestrial ingredients on the health of Atlantic salmon (Salmo salar) are poorly understood. During a 14-week trial, Atlantic salmon fed a fish meal-fish oil based diet (MAR) showed similar growth performance to others fed a plant protein/vegetable oil based diet (VEG), whereas poorer performance was observed in those fed an animal by-product meal/vegetable oil based diet (ABP). At the end of the trial, salmon were injected with either phosphate-buffered saline (PBS) or the viral mimic polyriboinosinic polyribocytidylic acid (pIC) and sampled for head kidney RNA after 24 h. The levels of 27 immune-related transcripts, and of 5 others involved in eicosanoid synthesis (including paralogues in both cases) were measured in the head kidney of the salmon using qPCR. All of the assayed immune-related genes and cox2 were pIC-induced, while the other eicosanoid synthesis-related genes were pIC-repressed. Linear regression was used to establish correlations between different immune transcripts, elucidating the cascade of responses to pIC and specialization among paralogues. Regarding the effect of diet on the antiviral immune response, pIC-treated fish fed diets ABP and VEG showed higher transcript levels of tlr3, irf1b, stat1a, isg15b, and gig1 compared to those fed diet MAR. We infer that the observed dietary immunomodulation could be due to the lower proportion of arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) in diets ABP and VEG. Furthermore, our results suggest a major role of dietary ARA in Atlantic salmon immunity, as low ARA proportion in diet VEG coincided with the highest pIC-induction of some immune transcripts (tlr7, stat1c, mxb, and gig1) and the lowest levels of transcripts encoding eicosanoid-synthesizing enzymes (5loxa, 5loxb, and pgds). In contrast, the high ARA/EPA ratio of diet ABP appeared to favor increased expression of transcripts involved in the synthesis of pro-inflammatory eicosanoids (5loxa and 5loxb) and chemotaxis (ccl19b). In conclusion, our findings show that nutritionally balanced plant-based diets may enhance the immune response of Atlantic salmon. Future studies should explore the possible advantages of plant-based diets in Atlantic salmon exposed to a viral infection.
[Mh] Termos MeSH primário: Ração Animal/análise
Dieta/veterinária
Imunidade Inata
Salmo salar/imunologia
[Mh] Termos MeSH secundário: Animais
Antivirais/administração & dosagem
Óleos de Peixe/administração & dosagem
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Rim Cefálico/imunologia
Rim Cefálico/metabolismo
Óleos Vegetais/administração & dosagem
Poli I-C/farmacologia
RNA/genética
RNA/metabolismo
Distribuição Aleatória
Salmo salar/genética
Proteínas de Vegetais Comestíveis/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Fish Oils); 0 (Fish Proteins); 0 (Plant Oils); 0 (Vegetable Proteins); 63231-63-0 (RNA); O84C90HH2L (Poly I-C)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE


  9 / 1616 MEDLINE  
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[PMID]:28103724
[Au] Autor:Budd GR; Aitchison A; Day AS; Keenan JI
[Ad] Endereço:1 Department of Surgery, University of Otago Christchurch, Christchurch, New Zealand.
[Ti] Título:The effect of polymeric formula on enterocyte differentiation.
[So] Source:Innate Immun;23(3):240-248, 2017 Apr.
[Is] ISSN:1753-4267
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Exclusive enteral nutrition is established as an initial therapy to induce remission in active Crohn's disease (CD), especially in children, but the mechanisms of action of this therapy are yet to be fully defined. Intestinal alkaline phosphatase (IAP), a recognised marker of enterocyte differentiation, is implicated in the innate gut immune response to enteric pathogens. Using the Caco-2 human adenocarcinoma cell line, this study showed that the incubation of human cells with a polymeric formula (PF) resulted in a dose-dependent increase in the expression of IAP on the cell surface. While further investigation is required to determine the pathway(s) involved, this finding suggests that cell surface-associated IAP may be an aspect of the gut's innate immune response to pathogenic bacteria that is strengthened by PF in the setting of CD.
[Mh] Termos MeSH primário: Fosfatase Alcalina/metabolismo
Carboidratos/farmacologia
Caseínas/farmacologia
Doença de Crohn/terapia
Nutrição Enteral
Enterócitos/efeitos dos fármacos
Lipídeos/farmacologia
Proteínas de Vegetais Comestíveis/farmacologia
[Mh] Termos MeSH secundário: Fosfatase Alcalina/genética
Células CACO-2
Diferenciação Celular/efeitos dos fármacos
Enterócitos/fisiologia
Alimentos Formulados
Proteínas Ligadas por GPI/genética
Proteínas Ligadas por GPI/metabolismo
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Imunidade Inata
Indução de Remissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Caseins); 0 (GPI-Linked Proteins); 0 (Lipids); 0 (Vegetable Proteins); 102257-18-1 (Osmolite); EC 3.1.3.1 (ALPI protein, human); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1177/1753425916689333


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[PMID]:27859974
[Au] Autor:Wang L; Oishi K; Sato Y; Nakanishi T; Hirooka H; Takahashi K; Kumagai H
[Ad] Endereço:Laboratory of Animal Husbandry Resources, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, Japan.
[Ti] Título:In vitro ruminal fermentation and in situ ruminal degradation of tamarind kernel powder extract residue in wethers.
[So] Source:Anim Sci J;88(7):966-973, 2017 Jul.
[Is] ISSN:1740-0929
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:We examined the in vitro fermentation characteristics and in situ dry matter (DM) and crude protein (CP) degradability of tamarind kernel powder extract residue (TKPER), a by-product of polysaccharides thickener processing. Two types of TKPER (I and II), of which the CP and neutral detergent fiber organic matter basis contents (%) were 41.4 and 42.0 and 1.4 and 0.5, respectively, were compared with dry heat soybean (SB), soybean meal (SBM) and dry soybean curd residue (SBCR). The TKPERs had significantly lower in vitro gas production compared to the other products at each observation time (P < 0.05). The in vitro DM and CP digestibility (%) of TKPER I and II were 67.7 and 64.9, and 64.5 and 58.0, respectively, significantly lower than those of SB and SBM (P < 0.05). We used four wethers (55.6 ± 4.6 kg) with ruminal cannulas to investigate the in situ degradability of TKPER I, SB, SBM and SBCR. At the ruminal outflow rate of 0.05/h, the effective degradability (%) values of the DM and CP of TKPER I were 64.1 and 49.7, respectively, which were similar to those of SBM. In conclusion, TKPER had high CP and exhibited in situ degradability similar to that of SBM, suggesting that TKPER could be used as a protein source feed.
[Mh] Termos MeSH primário: Ração Animal
Dieta/veterinária
Fermentação
Proteólise
Rúmen/metabolismo
Tamarindus
Proteínas de Vegetais Comestíveis/metabolismo
[Mh] Termos MeSH secundário: Animais
Técnicas In Vitro
Masculino
Extratos Vegetais
Pós
Ovinos
Feijão de Soja
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Powders); 0 (Vegetable Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1111/asj.12720



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