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Pesquisa : D12.776.260.643.415 [Categoria DeCS]
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[PMID]:28452458
[Au] Autor:Patel M; Moon HJ; Hong JH; Jeong B
[Ad] Endereço:Department of Chemistry and Nanoscience, Ewha Womans University , 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760 Korea.
[Ti] Título:Chiro-Optical Modulation for NURR1 Production from Stem Cells.
[So] Source:ACS Chem Neurosci;8(7):1455-1458, 2017 07 19.
[Is] ISSN:1948-7193
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nuclear receptor related 1 (NURR1) is an essential protein for maintenance of dopaminergic neurons in adult midbrain of which deficiency leads to Parkinson's disease. To enhance the NURR1 production of neural cells, various approaches are under investigation. Here we report that NURR1 is highly expressed in stem cells by exposure to an L-polarized blue light emitting diode (LED). Compared to stem cells cultured in the absence of a LED, under polarized green and red LEDs, the stem cells exposed to a polarized blue LED significantly enhanced neuronal biomarkers such as neurofilament M (NFM) and neuron specific enolase (NSE) at both mRNA and protein levels. In particular, NURR1 was selectively enhanced by the stem cells exposed to the L-polarized blue LED. Stem cells exposed to the L-polarized blue LED increased mitochondrial ATP and intracellular calcium ions, which support neuronal differentiation of the stem cells. This study suggests that chiro-optical treatments by using polarized light with a specific wavelength can be used for engineering of stem cells with enhanced specific biochemicals, which may open a new method for a specific disease.
[Mh] Termos MeSH primário: Luz
Células Mesenquimais Estromais/metabolismo
Neurogênese
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/biossíntese
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Cálcio/metabolismo
Técnicas de Cultura de Células/instrumentação
Sobrevivência Celular
Criança
Feminino
Imunofluorescência
Expressão Gênica/efeitos da radiação
Seres Humanos
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/efeitos da radiação
Mitocôndrias/metabolismo
Mitocôndrias/efeitos da radiação
Proteínas de Neurofilamentos/biossíntese
Proteínas de Neurofilamentos/genética
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/genética
Tonsila Palatina
Fosfopiruvato Hidratase/biossíntese
Fosfopiruvato Hidratase/genética
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (NR4A2 protein, human); 0 (Neurofilament Proteins); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (RNA, Messenger); 111365-29-8 (neurofilament protein M); 8L70Q75FXE (Adenosine Triphosphate); EC 4.2.1.11 (Phosphopyruvate Hydratase); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1021/acschemneuro.7b00136


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[PMID]:29254292
[Au] Autor:Cantore S; Ballini A; De Vito D; Martelli FS; Georgakopoulos I; Almasri M; Dibello V; Altini V; Farronato G; Dipalma G; Farronato D; Inchingolo F
[Ad] Endereço:Department of Interdisciplinary Medicine, School of Medicine, University of Bari Aldo Moro, Bari, Italy.
[Ti] Título:Characterization of human apical papilla-derived stem cells.
[So] Source:J Biol Regul Homeost Agents;31(4):901-910, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:Dental tissues represent an alternative and promising source of post-natal Mesenchymal stem cells (MSCs) for tissue engineering. Furthermore, dental stem cells from apical papilla (SCAPs) cells can be obtained from the wisdom tooth which is unnecessary for human masticatory function and frequently extracted for orthodontic reasons or dysodontiasis. More precisely, apical papilla is the immature, mostly uncalcified, precursor of the tooth root, therefore is composed of more undifferentiated cells than dental pulp. In addition, tooth extraction, especially by piezosurgery technique, can be considered less invasive in comparison to bone marrow or other tissues biopsy. Our work is aimed to investigate the safety of and predictable procedure on surgical immature third molar extraction and to provide new insight on SCAP research for future biomedical applications. The isolated cells were examined for stem cell properties by analyzing their colony-forming efficiency, differentiation characteristics and the expression of MSC markers.
[Mh] Termos MeSH primário: Polpa Dentária/citologia
Células Mesenquimais Estromais/citologia
Osteogênese/genética
Raiz Dentária/citologia
[Mh] Termos MeSH secundário: Adolescente
Biomarcadores/metabolismo
Diferenciação Celular
Proliferação Celular
Separação Celular
Criança
Ensaio de Unidades Formadoras de Colônias
Polpa Dentária/metabolismo
Feminino
Expressão Gênica
Seres Humanos
Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/genética
Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/metabolismo
Masculino
Células Mesenquimais Estromais/metabolismo
Dente Molar/cirurgia
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/genética
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo
Engenharia Tecidual
Extração Dentária
Raiz Dentária/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Insulin-Like Growth Factor Binding Proteins); 0 (JunB protein, human); 0 (NR4A2 protein, human); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (Transcription Factors)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


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[PMID]:29291419
[Au] Autor:Liu Q; Qin Q; Sun H; Zhong D; An R; Tian Y; Chen H; Jin J; Wang H; Li G
[Ad] Endereço:Department of Neurology, The First Affiliated Hospital of Harbin Medical University, China.
[Ti] Título:Neuroprotective effect of olfactory ensheathing cells co-transfected with Nurr1 and Ngn2 in both in vitro and in vivo models of Parkinson's disease.
[So] Source:Life Sci;194:168-176, 2018 Feb 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: The aim of the study is to evaluate the neuroprotective effects of olfactory ensheathing cells (OECs) with the overexpression of nuclear receptor-related factor 1 (Nurr1) and neurogenin 2 (Ngn2) in experimental models of Parkinson's disease (PD) and to elucidate the potential mechanism underlying the neuroprotective effects of OECs-Nurr1-Ngn2. MATERIALS AND METHODS: In vitro study, OECs-Nurr1-Ngn2 conditioned medium (CM) was added to MPP -treated PC12 cells for 24h, and then the viability of PC12 cells, oxidative stress and apoptosis were detected. In vivo study, 48 male Sprague-Dawley (SD) rats were randomly divided into four groups. OECs/VMCs and OECs-Nurr1-Ngn2/VMCs groups were transplanted with 2×10 cells each of OECs or OECs-Nurr1-Ngn2 and VMCs into the right striatum one week after a unilateral 6-OHDA lesion. Control and PD groups were injected with 0.9% NaCl and 0.2% ascorbic acid into the same region. Rotational behavior was determined at 2, 4, 6 and 8weeks after injection or implantation in all groups. Neuronal differentiation markers, oxidative stress- and apoptosis-related indicators were detected at 8weeks post-grafting. KEY FINDINGS: OECs-Nurr1-Ngn2 increased the viability of PC12 cells, inhibited oxidative stress and apoptosis, and these effects could be reversed by pre-treatment of k252a, a TrkB receptor inhibitor. The behavioral deficits of PD rat were ameliorated by the transplantation of OECs-Nurr1-Ngn2/VMCs. SIGNIFICANCE: These results suggest that OECs-Nurr1-Ngn2 exhibits substantial neuroprotective, anti-oxidant, and anti-apoptotic effects against PD via the up-regulation of the neurotrophic factor-TrkB pathway.
[Mh] Termos MeSH primário: Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Proteínas do Tecido Nervoso/genética
Neuroglia/transplante
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/genética
Bulbo Olfatório/citologia
Doença de Parkinson/metabolismo
Doença de Parkinson/terapia
Transfecção
[Mh] Termos MeSH secundário: Animais
Fatores de Transcrição Hélice-Alça-Hélice Básicos/uso terapêutico
Células Cultivadas
Modelos Animais de Doenças
Terapia Genética/métodos
Masculino
Proteínas do Tecido Nervoso/uso terapêutico
Neuroglia/metabolismo
Neuroproteção
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/uso terapêutico
Células PC12
Doença de Parkinson/genética
Doença de Parkinson/patologia
Ratos
Ratos Sprague-Dawley
Transfecção/métodos
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Nerve Tissue Proteins); 0 (Ngn2 protein, rat); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180102
[St] Status:MEDLINE


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[PMID]:27771535
[Au] Autor:Sim Y; Park G; Eo H; Huh E; Gu PS; Hong SP; Pak YK; Oh MS
[Ad] Endereço:Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
[Ti] Título:Protective effects of a herbal extract combination of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein.
[So] Source:Neuroscience;340:166-175, 2017 Jan 06.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parkinson's disease (PD) is one of the progressive neurodegenerative diseases of whose condition is characterized by dopaminergic neuronal cell loss and dysfunction in the substantia nigra pars compacta (SNpc) and the striatum. Recent studies have demonstrated that the nuclear receptor-related 1 protein (Nurr1) is critical of dopaminergic phenotype induction in mesencephalic dopaminergic neurons. Further, Nurr1 engages in synthesizing and storing dopamine through regulating levels of tyrosine hydroxylase (TH), dopamine transporter (DAT) and vesicular monoamine transporter 2 (VMAT2). The aim of this study was to investigate the protective effects of a herbal extract combination, consisting of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica (MABH), on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD-like symptoms and to elucidate possible mechanisms of action focusing on Nurr1. In a subacute mouse model of MPTP-induced PD, MABH treatment resulted in recovery from movement impairments. MABH prevented dopamine depletion and protected against dopaminergic neuronal degradation induced by MPTP. Additionally, MABH increased Nurr1 expression in the SNpc of mice. To evaluate the effects of MABH on Nurr1 expression, we measured the protein levels of Nurr1 and its regulating factors using Western blot analysis in PC12 cells. MABH treatment induced the phosphorylation of extracellular signal-regulated kinase protein via increasing the protein expression levels of Nurr1 and ultimately the levels of TH, VMAT2, and DAT. These results indicate that MABH has protective effects on dopaminergic neurons in a mouse model of PD by regulating Nurr1.
[Mh] Termos MeSH primário: Angelica
Bupleurum
Intoxicação por MPTP/tratamento farmacológico
Fármacos Neuroprotetores/farmacologia
Paeonia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Dopamina/metabolismo
Expressão Gênica/efeitos dos fármacos
Intoxicação por MPTP/metabolismo
Intoxicação por MPTP/patologia
Masculino
Camundongos Endogâmicos C57BL
Neurônios/efeitos dos fármacos
Neurônios/metabolismo
Neurônios/patologia
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo
Células PC12
Parte Compacta da Substância Negra/efeitos dos fármacos
Parte Compacta da Substância Negra/metabolismo
Parte Compacta da Substância Negra/patologia
Fitoterapia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Nr4a2 protein, mouse); 0 (Nr4a2 protein, rat); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (Plant Extracts); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171224
[Lr] Data última revisão:
171224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28808448
[Au] Autor:Malhotra SS; Gupta SK
[Ad] Endereço:Reproductive Cell Biology Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, -110 067 India.
[Ti] Título:Relevance of the NR4A sub-family of nuclear orphan receptors in trophoblastic BeWo cell differentiation.
[So] Source:Cell Mol Biol Lett;22:15, 2017.
[Is] ISSN:1689-1392
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nur-77, a member of the NR4A sub-family of nuclear orphan receptors, is downregulated in the placentae of pre-eclamptic women. Here, we investigate the relevance of Nor-1, Nurr-1 and Nur-77 in trophoblastic cell differentiation. Their transcript levels were found to be significantly upregulated in BeWo cells treated with forskolin. The maximum increase was observed after 2 h, with a second peak in the expression levels after 48 h. The expression of NR4A sub-family members was also found to be upregulated in BeWo cells after treatment with hCG and GnRH. A similar significant increase was observed at the respective protein levels after 2 and 48 h of treatment with forskolin, hCG or GnRH. Silencing Nor-1, Nurr-1 or Nur-77 individually did not show any effect on forskolin-, hCG- and/or GnRH-mediated BeWo cell fusion and/or hCG secretion. After silencing any one member of the NR4A sub-family, an increase in the transcript levels of the other sub-family members was observed, indicating a compensatory effect due to their functional redundancy. Simultaneously silencing all three NR4A sub-family members significantly downregulated forskolin- and hCG-mediated BeWo cell fusion and/or hCG secretion. However, a considerable amount of cell death occurred after forskolin or hCG treatment as compared to the control siRNA-transfected cells. These results suggest that the NR4A sub-family of nuclear orphan receptors has a role in trophoblastic cell differentiation.
[Mh] Termos MeSH primário: Diferenciação Celular
Receptores Nucleares Órfãos/fisiologia
Trofoblastos/metabolismo
[Mh] Termos MeSH secundário: Gonadotropina Coriônica Humana Subunidade beta/farmacologia
Colforsina/farmacologia
Regulação da Expressão Gênica no Desenvolvimento
Hormônio Liberador de Gonadotropina/farmacologia
Seres Humanos
Membro 1 do Grupo A da Subfamília 4 de Receptores Nucleares/genética
Membro 1 do Grupo A da Subfamília 4 de Receptores Nucleares/fisiologia
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/genética
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/fisiologia
Membro 3 do Grupo A da Subfamília 4 de Receptores Nucleares/genética
Membro 3 do Grupo A da Subfamília 4 de Receptores Nucleares/fisiologia
Receptores Nucleares Órfãos/genética
Trofoblastos/efeitos dos fármacos
Trofoblastos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chorionic Gonadotropin, beta Subunit, Human); 0 (Nuclear Receptor Subfamily 4, Group A, Member 1); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (Nuclear Receptor Subfamily 4, Group A, Member 3); 0 (Orphan Nuclear Receptors); 1F7A44V6OU (Colforsin); 33515-09-2 (Gonadotropin-Releasing Hormone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1186/s11658-017-0046-0


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[PMID]:28800627
[Au] Autor:Suzuki D; Saito-Hakoda A; Ito R; Shimizu K; Parvin R; Shimada H; Noro E; Suzuki S; Fujiwara I; Kagechika H; Rainey WE; Kure S; Ito S; Yokoyama A; Sugawara A
[Ad] Endereço:Department of Pediatrics, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
[Ti] Título:Suppressive effects of RXR agonist PA024 on adrenal CYP11B2 expression, aldosterone secretion and blood pressure.
[So] Source:PLoS One;12(8):e0181055, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effects of retinoids on adrenal aldosterone synthase gene (CYP11B2) expression and aldosterone secretion are still unknown. We therefore examined the effects of nuclear retinoid X receptor (RXR) pan-agonist PA024 on CYP11B2 expression, aldosterone secretion and blood pressure, to elucidate its potential as a novel anti-hypertensive drug. We demonstrated that PA024 significantly suppressed angiotensin II (Ang II)-induced CYP11B2 mRNA expression, promoter activity and aldosterone secretion in human adrenocortical H295R cells. Human CYP11B2 promoter functional analyses using its deletion and point mutants indicated that the suppression of CYP11B2 promoter activity by PA024 was in the region from -1521 (full length) to -106 including the NBRE-1 and the Ad5 elements, and the Ad5 element may be mainly involved in the PA024-mediated suppression. PA024 also significantly suppressed the Ang II-induced mRNA expression of transcription factors NURR1 and NGFIB that bind to and activate the Ad5 element. NURR1 overexpression demonstrated that the decrease of NURR1 expression may contribute to the PA024-mediated suppression of CYP11B2 transcription. PA024 also suppressed the Ang II-induced mRNA expression of StAR, HSD3ß2 and CYP21A2, a steroidogenic enzyme group involved in aldosterone biosynthesis. Additionally, the PA024-mediated CYP11B2 transcription suppression was shown to be exerted via RXRα. Moreover, the combination of PPARγ agonist pioglitazone and PA024 caused synergistic suppressive effects on CYP11B2 mRNA expression. Finally, PA024 treatment significantly lowered both the systolic and diastolic blood pressure in Tsukuba hypertensive mice (hRN8-12 x hAG2-5). Thus, RXR pan-agonist PA024 may be a candidate anti-hypertensive drugs that acts via the suppression of aldosterone synthesis and secretion.
[Mh] Termos MeSH primário: 2-Naftilamina/análogos & derivados
Córtex Suprarrenal/metabolismo
Aldosterona/secreção
Pressão Sanguínea/efeitos dos fármacos
Citocromo P-450 CYP11B2/metabolismo
Pirimidinas/farmacologia
Receptores X Retinoide/antagonistas & inibidores
[Mh] Termos MeSH secundário: 2-Naftilamina/farmacologia
Córtex Suprarrenal/efeitos dos fármacos
Animais
Apoptose/efeitos dos fármacos
Peso Corporal/efeitos dos fármacos
Cálcio/metabolismo
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Citocromo P-450 CYP11B2/genética
Frequência Cardíaca/efeitos dos fármacos
Seres Humanos
Hidrocortisona/secreção
Íons
Camundongos Transgênicos
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo
Mutação Puntual/genética
Regiões Promotoras Genéticas
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Receptores X Retinoide/metabolismo
Deleção de Sequência/genética
Esteroides/biossíntese
Tiazolidinedionas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ions); 0 (NR4A2 protein, human); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (PA024 compound); 0 (Pyrimidines); 0 (RNA, Messenger); 0 (Retinoid X Receptors); 0 (Steroids); 0 (Thiazolidinediones); 4964P6T9RB (Aldosterone); CKR7XL41N4 (2-Naphthylamine); EC 1.14.15.4 (Cytochrome P-450 CYP11B2); SY7Q814VUP (Calcium); WI4X0X7BPJ (Hydrocortisone); X4OV71U42S (pioglitazone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181055


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[PMID]:28637666
[Au] Autor:Prince LR; Prosseda SD; Higgins K; Carlring J; Prestwich EC; Ogryzko NV; Rahman A; Basran A; Falciani F; Taylor P; Renshaw SA; Whyte MKB; Sabroe I
[Ad] Endereço:Department of Infection, Immunity and Cardiovascular Disease and.
[Ti] Título:NR4A orphan nuclear receptor family members, NR4A2 and NR4A3, regulate neutrophil number and survival.
[So] Source:Blood;130(8):1014-1025, 2017 Aug 24.
[Is] ISSN:1528-0020
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lifespan of neutrophils is plastic and highly responsive to factors that regulate cellular survival. Defects in neutrophil number and survival are common to both hematologic disorders and chronic inflammatory diseases. At sites of inflammation, neutrophils respond to multiple signals that activate protein kinase A (PKA) signaling, which positively regulates neutrophil survival. The aim of this study was to define transcriptional responses to PKA activation and to delineate the roles of these factors in neutrophil function and survival. In human neutrophil gene array studies, we show that PKA activation upregulates a significant number of apoptosis-related genes, the most highly regulated of these being and Direct PKA activation by the site-selective PKA agonist pair N6/8-AHA (8-AHA-cAMP and N6-MB-cAMP) and treatment with endogenous activators of PKA, including adenosine and prostaglandin E2, results in a profound delay of neutrophil apoptosis and concomitant upregulation of NR4A2/3 in a PKA-dependent manner. NR4A3 expression is also increased at sites of neutrophilic inflammation in a human model of intradermal inflammation. PKA activation also promotes survival of murine neutrophil progenitor cells, and small interfering RNA to decreases neutrophil production in this model. Antisense knockdown of and homologs in zebrafish larvae significantly reduces the absolute neutrophil number without affecting cellular migration. In summary, we show that NR4A2 and NR4A3 are components of a downstream transcriptional response to PKA activation in the neutrophil, and that they positively regulate neutrophil survival and homeostasis.
[Mh] Termos MeSH primário: Neutrófilos/citologia
Neutrófilos/metabolismo
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo
Membro 3 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Contagem de Células
Proliferação Celular
Sobrevivência Celular
Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Dinoprostona/metabolismo
Ativação Enzimática
Deleção de Genes
Técnicas de Silenciamento de Genes
Seres Humanos
Inflamação/patologia
Larva/metabolismo
Camundongos
Família Multigênica
Análise de Sequência com Séries de Oligonucleotídeos
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Transdução de Sinais
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (Nuclear Receptor Subfamily 4, Group A, Member 3); 0 (RNA, Messenger); 0 (RNA, Small Interfering); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1182/blood-2017-03-770164


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[PMID]:28621822
[Au] Autor:Takahashi H; Tsuboi H; Asashima H; Hirota T; Kondo Y; Moriyama M; Matsumoto I; Nakamura S; Sumida T
[Ad] Endereço:Department of Internal Medicine, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
[Ti] Título:cDNA microarray analysis identifies NR4A2 as a novel molecule involved in the pathogenesis of Sjögren's syndrome.
[So] Source:Clin Exp Immunol;190(1):96-109, 2017 Oct.
[Is] ISSN:1365-2249
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To examine genes expressed specifically in labial salivary glands (LSGs) of patients with Sjögren's syndrome (SS) in comparison with those of patients with immunoglobulin (Ig)G4-related disease (IgG4-RD), and to identify the genes involved in the pathogenesis of SS. Gene expression in LSGs of SS patients, IgG4-RD patients and healthy controls (HC) was analysed by cDNA microarray. Quantitative polymerase chain reaction (qPCR) was used to validate the up-regulation of differentially expressed genes (DEGs) in SS. Protein production of the validated gene in LSGs was examined by immunofluorescence (IF) assay. The association of molecular functions of the gene with the pathological conditions in SS was examined using peripheral blood lymphocytes. Among 1320 DEGs up-regulated in SS, qPCR confirmed the up-regulation of NR4A2 in LSGs of SS compared with IgG4-RD. IF staining showed higher production of NR4A2 in nuclei of CD4 T cells and interleukin (IL)-17-producing cells in LSGs of SS, compared with IgG4-RD. Over-expression of NR4A2 mRNA was observed in peripheral CD4 T cells of SS patients, compared with HC. Nuclear NR4A2 expression in T helper type 17 (Th17)-polarized CD4 T cells determined by cellular IF was significantly higher in SS than in HC. Importazole, an inhibitor of importin-ß, inhibited nuclear transport of NR4A2 and Th17 polarization along with IL-21 expression in naive CD4 T cells under Th17-polarizing conditions, but did not alter retinoic acid receptor-related orphan receptor C (RORC) expression. NR4A2 seems to promote Th17 polarization via increased expression and intranuclear localization in CD4 T cells of SS patients, which could play a critical role in the pathogenesis of SS.
[Mh] Termos MeSH primário: Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo
Quinazolinas/uso terapêutico
Glândulas Salivares/fisiologia
Síndrome de Sjogren/metabolismo
Células Th17/imunologia
[Mh] Termos MeSH secundário: Transporte Ativo do Núcleo Celular/efeitos dos fármacos
Adulto
Idoso
Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
DNA Complementar/análise
Feminino
Perfilação da Expressão Gênica
Seres Humanos
Doenças do Sistema Imune/genética
Doenças do Sistema Imune/metabolismo
Imunoglobulina G/imunologia
Imunoglobulina G/metabolismo
Interleucinas/metabolismo
Meia-Idade
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/genética
Quinazolinas/farmacologia
Glândulas Salivares/patologia
Síndrome de Sjogren/tratamento farmacológico
Síndrome de Sjogren/genética
Células Th17/efeitos dos fármacos
Análise Serial de Tecidos/métodos
beta Carioferinas/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Immunoglobulin G); 0 (Interleukins); 0 (NR4A2 protein, human); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (Quinazolines); 0 (beta Karyopherins); 0 (importazole); 0 (interleukin-21)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1111/cei.13000


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[PMID]:28414330
[Au] Autor:Honeycutt JB; Thayer WO; Baker CE; Ribeiro RM; Lada SM; Cao Y; Cleary RA; Hudgens MG; Richman DD; Garcia JV
[Ad] Endereço:Division of Infectious Diseases, Center for AIDS Research, University of North Carolina, School of Medicine, Chapel Hill, North Carolina, USA.
[Ti] Título:HIV persistence in tissue macrophages of humanized myeloid-only mice during antiretroviral therapy.
[So] Source:Nat Med;23(5):638-643, 2017 May.
[Is] ISSN:1546-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite years of fully suppressive antiretroviral therapy (ART), HIV persists in its hosts and is never eradicated. One major barrier to eradication is that the virus infects multiple cell types that may individually contribute to HIV persistence. Tissue macrophages are critical contributors to HIV pathogenesis; however, their specific role in HIV persistence during long-term suppressive ART has not been established. Using humanized myeloid-only mice (MoM), we demonstrate that HIV infection of tissue macrophages is rapidly suppressed by ART, as reflected by a rapid drop in plasma viral load and a dramatic decrease in the levels of cell-associated viral RNA and DNA. No viral rebound was observed in the plasma of 67% of the ART-treated animals at 7 weeks after ART interruption, and no replication-competent virus was rescued from the tissue macrophages obtained from these animals. In contrast, in a subset of animals (∼33%), a delayed viral rebound was observed that is consistent with the establishment of persistent infection in tissue macrophages. These observations represent the first direct evidence, to our knowledge, of HIV persistence in tissue macrophages in vivo.
[Mh] Termos MeSH primário: Infecções por HIV/virologia
HIV-1/fisiologia
Macrófagos/virologia
[Mh] Termos MeSH secundário: Animais
Fármacos Anti-HIV/uso terapêutico
Terapia Antirretroviral de Alta Atividade
Medula Óssea
DNA Viral
Eletroforese em Gel de Campo Pulsado
Infecções por HIV/tratamento farmacológico
HIV-1/genética
Transplante de Células-Tronco Hematopoéticas
Seres Humanos
Imuno-Histoquímica
Lactonas
Leucócitos Mononucleares
Fígado
Macrófagos Alveolares/virologia
Camundongos
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares
Fenóis
RNA Viral
Baço
Linfócitos T
Carga Viral
Latência Viral
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-HIV Agents); 0 (DNA, Viral); 0 (Lactones); 0 (NR4A2 protein, human); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (Phenols); 0 (RNA, Viral); 141731-75-1 (NG 011)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1038/nm.4319


  10 / 646 MEDLINE  
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[PMID]:28177524
[Au] Autor:Goodings L; He J; Wood AJ; Harris WA; Currie PD; Jusuf PR
[Ad] Endereço:Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
[Ti] Título:In vivo expression of Nurr1/Nr4a2a in developing retinal amacrine subtypes in zebrafish Tg(nr4a2a:eGFP) transgenics.
[So] Source:J Comp Neurol;525(8):1962-1979, 2017 Jun 01.
[Is] ISSN:1096-9861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Nuclear receptor subfamily 4 group A member 2 (Nr4a2) is crucial for the formation or maintenance of dopaminergic neurons in the central nervous system including the retina, where dopaminergic amacrine cells contribute to visual function. Little is known about which cells express Nr4a2 at which developmental stage. Furthermore, whether Nr4a2 functions in combination with other genes is poorly understood. Thus, we generated a novel transgenic to visualize Nr4a2 expression in vivo during zebrafish retinogenesis. A 4.1 kb fragment of the nr4a2a promoter was used to drive green fluorescent protein expression in this Tg(nr4a2a:eGFP) line. In situ hybridization showed that transgene expression follows endogenous RNA expression at a cellular level. Temporal expression and lineages were quantified using in vivo time-lapse imaging in embryos. Nr4a2 expressing retinal subtypes were characterized immunohistochemically. Nr4a2a:eGFP labeled multiple neuron subtypes including 24.5% of all amacrine interneurons. Nr4a2a:eGFP labels all tyrosine hydroxylase labeled dopaminergic amacrine cells, and other nondopaminergic GABAergic amacrine populations. Nr4a2a:eGFP is confined to a specific progenitor lineage identified by sequential expression of the bhlh transcription factor Atonal7 (Atoh7) and Pancreas transcription factor 1a (Ptf1a), and labels postmitotic postmigratory amacrine cells. Thus, developmental Nr4a2a expression indicates a role during late differentiation of specific amacrine interneurons. Tg(nr4a2a:eGFP) is an early marker of distinct neurons including dopaminergic amacrine cells. It can be utilized to assess consequences of gene manipulations and understand whether Nr4a2 only carries out its role in the presence of specific coexpressed genes. This will allow Nr4a2 use to be refined for regenerative approaches.
[Mh] Termos MeSH primário: Células Amácrinas/citologia
Células Amácrinas/metabolismo
Neurogênese/fisiologia
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/biossíntese
Proteínas de Peixe-Zebra/biossíntese
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Diferenciação Celular/fisiologia
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Imuno-Histoquímica
Hibridização In Situ
Transcriptoma
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (Zebrafish Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1002/cne.24185



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