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[PMID]:28450830
[Au] Autor:Elliott KL; Kersigo J; Pan N; Jahan I; Fritzsch B
[Ad] Endereço:Department of Biology, University of IowaIowa City, IA, USA.
[Ti] Título:Spiral Ganglion Neuron Projection Development to the Hindbrain in Mice Lacking Peripheral and/or Central Target Differentiation.
[So] Source:Front Neural Circuits;11:25, 2017.
[Is] ISSN:1662-5110
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:We investigate the importance of the degree of peripheral or central target differentiation for mouse auditory afferent navigation to the organ of Corti and auditory nuclei in three different mouse models: first, a mouse in which the differentiation of hair cells, but not central auditory nuclei neurons is compromised ( ); second, a mouse in which hair cell defects are combined with a delayed defect in central auditory nuclei neurons ( ), and third, a mouse in which both hair cells and central auditory nuclei are absent ( ). Our results show that neither differentiated peripheral nor the central target cells of inner ear afferents are needed (hair cells, cochlear nucleus neurons) for segregation of vestibular and cochlear afferents within the hindbrain and some degree of base to apex segregation of cochlear afferents. These data suggest that inner ear spiral ganglion neuron processes may predominantly rely on temporally and spatially distinct molecular cues in the region of the targets rather than interaction with differentiated target cells for a crude topological organization. These developmental data imply that auditory neuron navigation properties may have evolved before auditory nuclei.
[Mh] Termos MeSH primário: Fatores de Transcrição Hélice-Alça-Hélice Básicos/deficiência
Diferenciação Celular/genética
Células Ciliadas Auditivas/fisiologia
Malformações do Sistema Nervoso/patologia
Fator de Transcrição PAX2/deficiência
Rombencéfalo/patologia
Gânglio Espiral da Cóclea
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Vias Auditivas/embriologia
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Núcleo Coclear/citologia
Núcleo Coclear/embriologia
Núcleo Coclear/crescimento & desenvolvimento
Embrião de Mamíferos
Camundongos
Camundongos Knockout
Malformações do Sistema Nervoso/genética
Fator de Transcrição PAX2/genética
Gânglio Espiral da Cóclea/embriologia
Gânglio Espiral da Cóclea/crescimento & desenvolvimento
Gânglio Espiral da Cóclea/patologia
beta-Galactosidase/genética
beta-Galactosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Atoh1 protein, mouse); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (PAX2 Transcription Factor); 0 (Pax2 protein, mouse); EC 3.2.1.23 (beta-Galactosidase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.3389/fncir.2017.00025


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[PMID]:29262504
[Au] Autor:Song Y; Zuo J; Huang X; Shen GH; Liu XY; Zhang X
[Ad] Endereço:Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peiking Union Medical College, Beijing 100021, China.
[Ti] Título:[Expressions and clinical significances of paired box gene 2 and cyclin D1 in advanced ovarian serous carcinoma].
[So] Source:Zhonghua Zhong Liu Za Zhi;39(12):891-895, 2017 Dec 23.
[Is] ISSN:0253-3766
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the expressions and clinical significances of paired box gene 2 (Pax2) and cyclin D1 protein in advanced ovarian serous carcinoma. From January 2003 to December 2013, the pathologic tissues of 202 patients with advanced ovarian serous cancer (â…¢-â…£) who underwent initial cytoreductive surgery were collected. The expressions of Pax2 and cyclin D1 protein were detected by immunohistochemistry in tissue microarray. The relationships of their expressions with the clinicopathological features and prognosis of the patients were analyzed. The positive rate of Pax2 protein expression of the 202 patients with ovarian serous adenocarcinoma was 24.8% (50/202) and that of cyclin D1 was 25.2% (51/202). The expressions of Pax2 and cyclin D1 were not significantly related with age, clinical stage and pathological grade of ovarian serous adenocarcinoma patients ( >0.05). The median overall survival (OS) time of Pax2-negative patients was 53 months and the progression-free survival (PFS) time was 29 months. The median OS time of Pax2-positive patients was 66 months and PFS time was 33 months, the OS of Pax2-negative patients was significant different from that of Pax2-positive patients ( (2)=4.06, =0.04). The median PFS time of Pax2-negative patients was not significant different from that of Pax2-positive patients ( (2)=2.43, =0.11). The median OS time of cyclin D1-negative patients was 62 months and PFS time was 30 months. The median OS time of cyclin D1-positive patients was 48 months and PFS time was 22 months. The median OS time of cyclin D1-negative patients was significantly different from that of cyclin D1-positive patients ( (2)=4.71, =0.03), while the median PFS time of cyclin D1-negative patients was marginally different from that of cyclin D1-positive patients ( (2)=0.59, =0.41). Multivariate analysis showed that the expression of Pax2 was an independent factor of the prognosis for patients with ovarian serous adenocarcinoma ( =0.597, 95% 0.371-0.962, <0.034). The expressions of Pax2 and cyclin D1 are associated with the prognosis of patients with advanced ovarian serous adenocarcinoma while Pax2 is an independent prognostic factor.
[Mh] Termos MeSH primário: Ciclina D1/metabolismo
Cistadenocarcinoma Seroso/metabolismo
Neoplasias Ovarianas/metabolismo
Fator de Transcrição PAX2/metabolismo
[Mh] Termos MeSH secundário: Cistadenocarcinoma Seroso/patologia
Intervalo Livre de Doença
Feminino
Seres Humanos
Imuno-Histoquímica
Neoplasias Ovarianas/patologia
Prognóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCND1 protein, human); 0 (PAX2 Transcription Factor); 136601-57-5 (Cyclin D1)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0253-3766.2017.12.003


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[PMID]:29054766
[Au] Autor:Liu J; Wang P; Huang J; Yu Z
[Ad] Endereço:Department of Pediatrics, Fuzhou Dongfang Hospital, Second Military Medical University, Fuzhou 350025, Fujian, China.
[Ti] Título:Rethinking genotype-phenotype correlations in papillorenal syndrome: a case report on an unusual congenital camptodactyly and skeletal deformity with a heterogeneous PAX2 mutation of hexanucleotide duplication.
[So] Source:Gene;641:74-77, 2018 Jan 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Papillorenal syndrome (PRS), an autosomal dominant inherited condition, is clinically featured by renal hypoplasia and optic nerve dysplasia. Based on current knowledge of genotype-phenotype correlations in PRS, mutations in the Paired box 2 (PAX2) gene have been recognized as a critical pathogenesis of typical renal and optic disease manifestations. However, little information is currently available on the skeletal abnormalities of PRS and the potential contribution of PAX2 mutations. Here, we present a case of a 10-year-old female PRS patient with the typical features of chronic renal failure and severe myopia, but was unexpectedly discovered camptodactyly of her left middle finger which affects the proximal interphalangeal joint. Pathologically, the camptodactyly was further indicated by radiology as a skeletal deformity, demonstrating a decline of bone mineral density and disappearance of joint space. Molecular diagnostics revealed a heterozygous mutation, 220_225dup, in the exon 3 of her PAX2 gene, which is de novo considering the lack of this mutation in her non-consanguineous parents. This mutation leads to duplication of glutamic acid at position 74 and tyrosine at position 75 in PAX2 protein, which may influence the DNA-binding function. Besides, the absence of Spalt like transcription factor 4 (SALL4) mutation excluded the diagnosis of acro-renal-ocular syndrome (AROS), of which clinical characteristics are similar to our patient's. This case unravels a previously unrecognized phenotype of camptodactyly due to a significant skeletal deformity of PRS with a heterogeneous PAX2 mutation of hexanucleotide duplication. This report challenges against the current belief of genotype-phenotype correlations in PRS.
[Mh] Termos MeSH primário: Coloboma/genética
Articulações dos Dedos/anormalidades
Deformidades Congênitas da Mão/genética
Fator de Transcrição PAX2/genética
Insuficiência Renal/genética
Refluxo Vesicoureteral/genética
[Mh] Termos MeSH secundário: Densidade Óssea/genética
Criança
Coloboma/patologia
Feminino
Seres Humanos
Falência Renal Crônica/genética
Miopia/genética
Insuficiência Renal/patologia
Fatores de Transcrição/genética
Refluxo Vesicoureteral/patologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (PAX2 Transcription Factor); 0 (PAX2 protein, human); 0 (SALL4 protein, human); 0 (Transcription Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171022
[St] Status:MEDLINE


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[PMID]:29060967
[Au] Autor:Mao YN; Zeng LX; Li YH; Liu YZ; Wu JY; Li L; Wang Q
[Ad] Endereço:Laboratory of Early Prevention and Treatment for Regional High Frequence Tumor Ministry of Education Key Laboratory, Affiliated Tumor Hospital, Guangxi Medical University, Nanning 530021, China.
[Ti] Título:[Significance and expression of PAX8, PAX2, p53 and RAS in ovary and fallopian tubes to origin of ovarian high grade serous carcinoma].
[So] Source:Zhonghua Fu Chan Ke Za Zhi;52(10):687-696, 2017 Oct 25.
[Is] ISSN:0529-567X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To explore the origin of ovarian high grade serous carcinoma (HGSC) through analysing the expression and significance of PAX8, PAX2, p53 and RAS in the ovary and fallopian tube of different types and grades of serous carcinoma. A total of 44 cases tissue samples of ovarian tumor including 34 malignant ovarian tumor and 10 normal normal tissue (as control group) were collected from the admitted patients in Affiliated Tumor Hospital of Guangxi Medical University from January 2015 to January 2016. Fallopian tube tissues were segmented in accordance with the fimbria, ampulla, isthmus and the corresponding ovarian tissues were by the side. There were 34 cases of patients with ovarian cancer including 29 cases of epithelial ovarian cancer (27 serous carcinoma, 1 mucinous carcinoma,1 endometrioid adenocarcinoma) and 5 non-epithelial ovarian cancer (sex cord-interstitial tumor). Among 27 cases of patients with ovarian serous cancer, there were 23 HGSC and 4 low-grade ovarian serous cancer (LGSC). One hundred fifty-three cases of samples were diagnosed as ovarian serous cancer by Shandong University Affiliated Qilu Hospital from 2005 to 2013 and these samples were made tissue microarray. (1) To analyze the expression and differences of PAX8, PAX2, p53 and RAS in the above tissues and tissue microarray from ovarian and tubal of HGSC and control women by immunohistochemistry methods. (2) To compare the expression levels of PAX8, PAX2, p53 and RAS in ovarian and fallopian tubes of ovarian cancer patients with different pathological types. (3) To analyze the correlations of tubal and ovarian tissue in PAX8, PAX2, p53 and RAS expression of HGSC. (4) To analyze the factors of the prognosis of ovarian serous cancer in tissue microarray by single factor analysis method. (1) PAX8, PAX2, p53 and RAS expression was negative in normal ovarian epithelium of control group, but the expression of PAX8, PAX2, p53 and RAS were strongly positive brown in secrete cells of normal fallopian tube epithelium. (2) p53 and RAS expression of fallopian tube epithelium in the epithelial ovarian cancer group were significantly higher than those in the non-epithelial ovarian cancer groups ( 0.05), but the expression of PAX8 and PAX2 in fallopian tube and the expression of PAX8, PAX2, p53 and RAS in ovarian tissue was not statistically significant in the groups ( 0.05). PAX8, PAX2 and p53 expression of the ovarian in HGSC group were significantly higher than those in LGSC group ( 0.05), while the expression of RAS was lower in the ovarian of the high-grade group ( 0.05), while the expression of PAX8, PAX2, p53 and RAS in fallopian tube was not statistically significant in the groups ( 0.05). (3) There was a significantly positive correlation between fallopian tube and the corresponding ovary of HGSC in PAX8 and PAX2 expression ( 0.422, 0.045; 0.693, 0.000), but not correlation in p53 and RAS expression ( 0.058, 0.793; 0.190, 0.384). (4) Univariate survival analysis showed that the progression free survival time in patients with ovarian serous cancer group was significantly correlated with the protein expression of PAX8, PAX2 and RAS ( 0.05), but there were not correlated with age, surgical staging, cell differentiation, lymph node metastasis and preoperative chemotherapy and p53 protein expression ( 0.05). The total survival time in patients with ovarian serous cancer group was significantly correlated with the protein expression of PAX8 ( 0.05), but there were not correlated with age,surgical staging, cell differentiation, lymph node metastasis and preoperative chemotherapy and the protein expression of PAX2, RAS and p53 ( 0.05). PAX8, PAX2, p53, RAS are of great significance for the study of origin of HGSC. HGSC may be derived from fallopian tube, but further investigation would be necessary to confirm this. PAX8, PAX2, p53, RAS could be expected to be used as predictors of survival prognosis in patients with ovarian serous cancer.
[Mh] Termos MeSH primário: Cistadenocarcinoma Seroso/patologia
Neoplasias das Tubas Uterinas/patologia
Tubas Uterinas/patologia
Proteínas Monoméricas de Ligação ao GTP/metabolismo
Neoplasias Epiteliais e Glandulares/patologia
Neoplasias Ovarianas/patologia
Fator de Transcrição PAX2/metabolismo
Fator de Transcrição PAX8/metabolismo
Proteína Supressora de Tumor p53/metabolismo
[Mh] Termos MeSH secundário: Carcinoma Endometrioide
China
Cistadenocarcinoma Seroso/genética
Cistadenocarcinoma Seroso/metabolismo
Epitélio
Neoplasias das Tubas Uterinas/genética
Neoplasias das Tubas Uterinas/metabolismo
Tubas Uterinas/metabolismo
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Imuno-Histoquímica
Proteínas Monoméricas de Ligação ao GTP/genética
Proteínas de Neoplasias/genética
Proteínas de Neoplasias/metabolismo
Neoplasias Epiteliais e Glandulares/genética
Neoplasias Epiteliais e Glandulares/metabolismo
Neoplasias Ovarianas/genética
Neoplasias Ovarianas/metabolismo
Fator de Transcrição PAX2/genética
Fator de Transcrição PAX8/genética
Proteína Supressora de Tumor p53/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neoplasm Proteins); 0 (PAX2 Transcription Factor); 0 (PAX2 protein, human); 0 (PAX8 Transcription Factor); 0 (PAX8 protein, human); 0 (Tumor Suppressor Protein p53); EC 3.6.5.2 (Monomeric GTP-Binding Proteins); EC 3.6.5.2 (REM2 protein, human)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-567X.2017.10.008


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[PMID]:28689736
[Au] Autor:Sedykh I; Yoon B; Roberson L; Moskvin O; Dewey CN; Grinblat Y
[Ad] Endereço:Department of Integrative Biology, University of Wisconsin, Madison, WI 53706, USA; Department of Neuroscience, University of Wisconsin, Madison, WI 53706, USA.
[Ti] Título:Zebrafish zic2 controls formation of periocular neural crest and choroid fissure morphogenesis.
[So] Source:Dev Biol;429(1):92-104, 2017 09 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The vertebrate retina develops in close proximity to the forebrain and neural crest-derived cartilages of the face and jaw. Coloboma, a congenital eye malformation, is associated with aberrant forebrain development (holoprosencephaly) and with craniofacial defects (frontonasal dysplasia) in humans, suggesting a critical role for cross-lineage interactions during retinal morphogenesis. ZIC2, a zinc-finger transcription factor, is linked to human holoprosencephaly. We have previously used morpholino assays to show zebrafish zic2 functions in the developing forebrain, retina and craniofacial cartilage. We now report that zebrafish with genetic lesions in zebrafish zic2 orthologs, zic2a and zic2b, develop with retinal coloboma and craniofacial anomalies. We demonstrate a requirement for zic2 in restricting pax2a expression and show evidence that zic2 function limits Hh signaling. RNA-seq transcriptome analysis identified an early requirement for zic2 in periocular neural crest as an activator of alx1, a transcription factor with essential roles in craniofacial and ocular morphogenesis in human and zebrafish. Collectively, these data establish zic2 mutant zebrafish as a powerful new genetic model for in-depth dissection of cell interactions and genetic controls during craniofacial complex development.
[Mh] Termos MeSH primário: Corioide/embriologia
Corioide/metabolismo
Morfogênese
Crista Neural/metabolismo
Fatores de Transcrição/metabolismo
Proteínas de Peixe-Zebra/metabolismo
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Cartilagem/efeitos dos fármacos
Cartilagem/metabolismo
Linhagem da Célula/efeitos dos fármacos
Linhagem da Célula/genética
Coloboma/patologia
Face/embriologia
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Morfogênese/efeitos dos fármacos
Morfogênese/genética
Mutação/genética
Crista Neural/citologia
Crista Neural/efeitos dos fármacos
Fator de Transcrição PAX2/genética
Fator de Transcrição PAX2/metabolismo
Retina/efeitos dos fármacos
Retina/embriologia
Análise de Sequência de RNA
Homologia de Sequência de Aminoácidos
Crânio/embriologia
Fatores de Transcrição/genética
Alcaloides de Veratrum/farmacologia
Peixe-Zebra/embriologia
Peixe-Zebra/genética
Proteínas de Peixe-Zebra/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (PAX2 Transcription Factor); 0 (Transcription Factors); 0 (Veratrum Alkaloids); 0 (Zebrafish Proteins); 0 (Zic2a protein, zebrafish); 0 (pax2a protein, zebrafish); ZH658AJ192 (cyclopamine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE


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[PMID]:28635231
[Au] Autor:Song Y; Huang X; Shen GH; Liu XY; Zhang X
[Ad] Endereço:Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
[Ti] Título:[Comparison of paired box genes 8 and 2 expression in epithelium tissues and the related tumors].
[So] Source:Zhonghua Zhong Liu Za Zhi;39(6):424-428, 2017 Jun 23.
[Is] ISSN:0253-3766
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To explore the expressional differences between paired box genes 2(Pax2) and 8 (Pax8) protein in different kinds of epitheliums and tumors, and to investigate the clinicopathologic significance. Expression levels of Pax2 and Pax8 protein were detected in 75 cases of different human epithelium tissues and 255 cases of different tumors on tissue microarray by immunohistochemistry. Pax2 and Pax8 selectively expressed in different tissues. The positive rates of Pax8 protein expressed in the normal epithelium of the thyroid, urinary system and female reproductive system were 100% (2/2), 60.0% (3/5) and 76.9% (10/13), respectively. The positive rates of Pax2 expressed in the epithelium tissues of urinary system and the female reproductive system were 40.0% (2/5) and 38.5% (5/13) respectively. However, the expression of Pax2 protein was not detected in the normal thyroid epithelium. The positive rate of Pax8 protein expressing in the epithelium of reproductive system was significantly higher than that of Pax2 protein ( <0.05). The tumors derived from different tissues also expressed different levels of protein Pax2 and Pax8. The positive rates of Pax8 in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were 65.2% (15/23), 66.7% (10/15) and 80.0% (4/5), respectively. The positive rates of Pax2 in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were 34.8% (8/23), 13.3% (2/15) and 20.0% (1/5), respectively. The positive rates of Pax8 protein expressed in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were significantly higher than those of Pax2 protein ( <0.05). The positive rates of Pax8 in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinoma were 92.9% (26/28), 81.8% (9/11) and 82.4% (14/17), respectively. The positive rates of Pax2 in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinoma were 28.6% (8/28), 9.1% (1/11) and 17.6% (3/17), respectively. The positive rates of Pax8 protein expressed in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinomawere significantly higher than those of Pax2 protein ( <0.05). Pax2 and Pax8 are specifically expressed in female reproductive system and uritany system. However, the positive expression of Pax8 is superior to that of Pax2. The combined expression of Pax8 and Pax2 can be used in the differential diagnosis of epithelial tumors derived from different origins.
[Mh] Termos MeSH primário: Epitélio/metabolismo
Expressão Gênica
Proteínas de Neoplasias/genética
Fator de Transcrição PAX2/genética
Fator de Transcrição PAX8/genética
[Mh] Termos MeSH secundário: Adenocarcinoma de Células Claras/metabolismo
Carcinoma de Células Renais/metabolismo
Diagnóstico Diferencial
Feminino
Genitália Feminina/metabolismo
Seres Humanos
Imuno-Histoquímica
Masculino
Proteínas de Neoplasias/metabolismo
Neoplasias Epiteliais e Glandulares/metabolismo
Especificidade de Órgãos
Neoplasias Ovarianas/metabolismo
Fator de Transcrição PAX2/metabolismo
Fator de Transcrição PAX8/metabolismo
Glândula Tireoide/metabolismo
Análise Serial de Tecidos
Sistema Urinário/metabolismo
Neoplasias Uterinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neoplasm Proteins); 0 (PAX2 Transcription Factor); 0 (PAX8 Transcription Factor)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0253-3766.2017.06.005


  7 / 780 MEDLINE  
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[PMID]:28615245
[Au] Autor:Ye H; Wang X; Constans MM; Sussman CR; Chebib FT; Irazabal MV; Young WF; Harris PC; Kirschner LS; Torres VE
[Ad] Endereço:Mayo Clinic, Rochester Minnesota; and.
[Ti] Título:The regulatory 1α subunit of protein kinase A modulates renal cystogenesis.
[So] Source:Am J Physiol Renal Physiol;313(3):F677-F686, 2017 Sep 01.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The failure of the polycystins (PCs) to function in primary cilia is thought to be responsible for autosomal dominant polycystic kidney disease (ADPKD). Primary cilia integrate multiple cellular signaling pathways, including calcium, cAMP, Wnt, and Hedgehog, which control cell proliferation and differentiation. It has been proposed that mutated PCs result in reduced intracellular calcium, which in turn upregulates cAMP, protein kinase A (PKA) signaling, and subsequently other proliferative signaling pathways. However, the role of PKA in ADPKD has not been directly ascertained in vivo, although the expression of the main regulatory subunit of PKA in cilia and other compartments (PKA-RIα, encoded by ) is increased in a mouse model orthologous to ADPKD. Therefore, we generated a kidney-specific knockout of to examine the consequences of constitutive upregulation of PKA on wild-type and hypomorphic ( ) backgrounds. Kidney-specific loss of induced renal cystic disease and markedly aggravated cystogenesis in the models. In both settings, it was accompanied by upregulation of Src, Ras, MAPK/ERK, mTOR, CREB, STAT3, Pax2 and Wnt signaling. On the other hand, Gli3 repressor activity was enhanced, possibly contributing to hydronephrosis and impaired glomerulogenesis in some animals. To assess the relevance of these observations in humans we looked for and found evidence for kidney and liver cystic phenotypes in the Carney complex, a tumoral syndrome caused by mutations in These observations expand our understanding of the pathogenesis of ADPKD and demonstrate the importance of highlighting PKA as a therapeutic target in ADPKD.
[Mh] Termos MeSH primário: Complexo de Carney/enzimologia
Subunidade RIalfa da Proteína Quinase Dependente de AMP Cíclico/metabolismo
Cistos/enzimologia
Rim/enzimologia
Hepatopatias/enzimologia
Rim Policístico Autossômico Dominante/enzimologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Complexo de Carney/diagnóstico por imagem
Complexo de Carney/genética
Proliferação Celular
Criança
Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo
Subunidade RIalfa da Proteína Quinase Dependente de AMP Cíclico/deficiência
Subunidade RIalfa da Proteína Quinase Dependente de AMP Cíclico/genética
Cistos/diagnóstico por imagem
Cistos/genética
Modelos Animais de Doenças
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Feminino
Predisposição Genética para Doença
Seres Humanos
Rim/patologia
Rim/fisiopatologia
Fatores de Transcrição Kruppel-Like/genética
Fatores de Transcrição Kruppel-Like/metabolismo
Hepatopatias/diagnóstico por imagem
Hepatopatias/genética
Masculino
Camundongos da Linhagem 129
Camundongos Endogâmicos C57BL
Camundongos Knockout
Meia-Idade
Proteínas do Tecido Nervoso/genética
Proteínas do Tecido Nervoso/metabolismo
Fator de Transcrição PAX2/metabolismo
Fenótipo
Rim Policístico Autossômico Dominante/genética
Rim Policístico Autossômico Dominante/patologia
Rim Policístico Autossômico Dominante/fisiopatologia
Fator de Transcrição STAT3/metabolismo
Serina-Treonina Quinases TOR/metabolismo
Canais de Cátion TRPP/genética
Via de Sinalização Wnt
Adulto Jovem
Proteína Gli3 com Dedos de Zinco
Proteínas ras/metabolismo
Quinases da Família src/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Creb1 protein, mouse); 0 (Cyclic AMP Response Element-Binding Protein); 0 (Cyclic AMP-Dependent Protein Kinase RIalpha Subunit); 0 (Gli3 protein, mouse); 0 (Kruppel-Like Transcription Factors); 0 (Nerve Tissue Proteins); 0 (PAX2 Transcription Factor); 0 (PRKAR1A protein, human); 0 (Pax2 protein, mouse); 0 (Prkar1a protein, mouse); 0 (STAT3 Transcription Factor); 0 (Stat3 protein, mouse); 0 (TRPP Cation Channels); 0 (Zinc Finger Protein Gli3); 0 (polycystic kidney disease 1 protein); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.1.1 (mTOR protein, mouse); EC 2.7.10.2 (src-Family Kinases); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 3.6.5.2 (ras Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00119.2017


  8 / 780 MEDLINE  
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[PMID]:28576690
[Au] Autor:Grajales-Esquivel E; Luz-Madrigal A; Bierly J; Haynes T; Reis ES; Han Z; Gutierrez C; McKinney Z; Tzekou A; Lambris JD; Tsonis PA; Del Rio-Tsonis K
[Ad] Endereço:Department of Biology, Miami University and Center for Visual Sciences at Miami University (CVSMU), Oxford, OH 45056, USA. Electronic address: esquiveg@miamioh.edu.
[Ti] Título:Complement component C3aR constitutes a novel regulator for chick eye morphogenesis.
[So] Source:Dev Biol;428(1):88-100, 2017 08 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Complement components have been implicated in a wide variety of functions including neurogenesis, proliferation, cell migration, differentiation, cancer, and more recently early development and regeneration. Following our initial observations indicating that C3a/C3aR signaling induces chick retina regeneration, we analyzed its role in chick eye morphogenesis. During eye development, the optic vesicle (OV) invaginates to generate a bilayer optic cup (OC) that gives rise to the retinal pigmented epithelium (RPE) and neural retina. We show by immunofluorescence staining that C3 and the receptor for C3a (the cleaved and active form of C3), C3aR, are present in chick embryos during eye morphogenesis in the OV and OC. Interestingly, C3aR is mainly localized in the nuclear compartment at the OC stage. Loss of function studies at the OV stage using morpholinos or a blocking antibody targeting the C3aR (anti-C3aR Ab), causes eye defects such as microphthalmia and defects in the ventral portion of the eye that result in coloboma. Such defects were not observed when C3aR was disrupted at the OC stage. Histological analysis demonstrated that microphthalmic eyes were unable to generate a normal optic stalk or a closed OC. The dorsal/ventral patterning defects were accompanied by an expansion of the ventral markers Pax2, cVax and retinoic acid synthesizing enzyme raldh-3 (aldh1a3) domains, an absence of the dorsal expression of Tbx5 and raldh-1 (aldh1a1) and a re-specification of the ventral RPE to neuroepithelium. In addition, the eyes showed overall decreased expression of Gli1 and a change in distribution of nuclear ß-catenin, suggesting that Shh and Wnt pathways have been affected. Finally, we observed prominent cell death along with a decrease in proliferating cells, indicating that both processes contribute to the microphthalmic phenotype. Together our results show that C3aR is necessary for the proper morphogenesis of the OC. This is the first report implicating C3aR in eye development, revealing an unsuspected hitherto regulator for proper chick eye morphogenesis.
[Mh] Termos MeSH primário: Padronização Corporal/fisiologia
Complemento C3a/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Receptores de Complemento/metabolismo
Epitélio Pigmentado da Retina/embriologia
[Mh] Termos MeSH secundário: Aldeído Desidrogenase/metabolismo
Animais
Apoptose/fisiologia
Proliferação Celular/fisiologia
Embrião de Galinha
Proteínas Hedgehog/metabolismo
Microftalmia/embriologia
Morfogênese/fisiologia
Fator de Transcrição PAX2/metabolismo
Receptores de Complemento/genética
Retinal Desidrogenase/metabolismo
Proteínas com Domínio T-Box/metabolismo
Via de Sinalização Wnt/fisiologia
Proteína GLI1 em Dedos de Zinco/biossíntese
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Hedgehog Proteins); 0 (PAX2 Transcription Factor); 0 (Receptors, Complement); 0 (T-Box Domain Proteins); 0 (T-box transcription factor 5); 0 (Zinc Finger Protein GLI1); 0 (beta Catenin); 0 (complement C3a receptor); 80295-42-7 (Complement C3a); EC 1.2.1.3 (Aldehyde Dehydrogenase); EC 1.2.1.36 (Retinal Dehydrogenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE


  9 / 780 MEDLINE  
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[PMID]:28535371
[Au] Autor:Naiman N; Fujioka K; Fujino M; Valerius MT; Potter SS; McMahon AP; Kobayashi A
[Ad] Endereço:Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
[Ti] Título:Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
[So] Source:Dev Cell;41(4):349-365.e3, 2017 May 22.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The kidney contains the functional units, the nephrons, surrounded by the renal interstitium. Previously we discovered that, once Six2-expressing nephron progenitor cells and Foxd1-expressing renal interstitial progenitor cells form at the onset of kidney development, descendant cells from these populations contribute exclusively to the main body of nephrons and renal interstitial tissues, respectively, indicating a lineage boundary between the nephron and renal interstitial compartments. Currently it is unclear how lineages are regulated during kidney organogenesis. We demonstrate that nephron progenitor cells lacking Pax2 fail to differentiate into nephron cells but can switch fates into renal interstitium-like cell types. These data suggest that Pax2 function maintains nephron progenitor cells by repressing a renal interstitial cell program. Thus, the lineage boundary between the nephron and renal interstitial compartments is maintained by the Pax2 activity in nephron progenitor cells during kidney organogenesis.
[Mh] Termos MeSH primário: Padronização Corporal
Néfrons/citologia
Néfrons/embriologia
Fator de Transcrição PAX2/metabolismo
Células-Tronco/citologia
Células-Tronco/metabolismo
[Mh] Termos MeSH secundário: Alelos
Animais
Padronização Corporal/genética
Transdiferenciação Celular
Fatores de Transcrição Forkhead/metabolismo
Perfilação da Expressão Gênica
Proteínas de Homeodomínio/metabolismo
Mesoderma/citologia
Mesoderma/embriologia
Mesoderma/metabolismo
Camundongos Endogâmicos C57BL
Néfrons/metabolismo
Organogênese/genética
Análise de Sequência de RNA
Análise de Célula Única
Células Estromais/metabolismo
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Forkhead Transcription Factors); 0 (Foxd1 protein, mouse); 0 (Homeodomain Proteins); 0 (PAX2 Transcription Factor); 0 (Six2 protein, mouse); 0 (Transcription Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE


  10 / 780 MEDLINE  
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[PMID]:28535367
[Au] Autor:Davies J
[Ad] Endereço:College of Medicine, University of Edinburgh, Edinburgh EH8 9XB, UK. Electronic address: jamie.davies@ed.ac.uk.
[Ti] Título:Pax2: A "Keep to the Path" Sign on Waddington's Epigenetic Landscape.
[So] Source:Dev Cell;41(4):331-332, 2017 05 22.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Developing kidneys have Foxd1 stromogenic stem cells and [Six2 , Pax2 ] nephrogenic stem cells. In this issue of Developmental Cell, Naiman et al. (2017) show that targeted Pax2 deletion converts nephrogenic cells to the stromogenic path, suggesting that Pax2 normally represses an otherwise inevitable transition between sister lineages.
[Mh] Termos MeSH primário: Epigenômica
Fator de Transcrição PAX2
[Mh] Termos MeSH secundário: Organogênese
Células-Tronco
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Nm] Nome de substância:
0 (PAX2 Transcription Factor)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE



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