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Pesquisa : D12.776.325 [Categoria DeCS]
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[PMID]:29339070
[Au] Autor:Wang R; Li Y; Zhou Z; Liu Q; Zeng L; Xiao T
[Ad] Endereço:Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China; College of Life and Environment Sciences, Hunan University of Arts and Science, Changde, Hunan 415000, China.
[Ti] Título:Involvement of interferon regulatory factor 3 from the barbel chub Squaliobarbus curriculus in the immune response against grass carp reovirus.
[So] Source:Gene;648:5-11, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The barbel chub Squaliobarbus curriculus is an important commercial fish species in China, and has shown significant resistance to grass carp reovirus (GCRV). In this study, the cDNA sequence of interferon regulatory factors 3 (IRF3) from Squaliobarbus curriculus, designated as ScIRF3, was cloned, and its effect against GCRV was investigated. The full-length 1837 base pair (bp) cDNA of ScIRF3 contained a complete open reading frame of 1374 bp and encoded a putative polypeptide of 457 amino acid residues. The ScIRF3 protein contained conserved domains, including an N-terminal DNA-binding domain, a C-terminal IRF association domain, and a serine-rich domain. Phylogenetic analysis showed that ScIRF3 was closely clustered with IRF3s from Carassius auratus and Ctenopharyngodon idellus. Quantitative real-time polymerase chain reaction analysis showed that the expression levels of ScIRF3 in Squaliobarbus curriculus were the highest in the spleen and lowest in the muscle. After GCRV infection, expression levels of both ScIRF3 and type I interferon (IFN) were initially up-regulated and subsequently down-regulated in the spleen and intestine. Correlation analysis showed that the expression level of type I IFN is significantly positively correlated with that of ScIRF3 (Pearson correlation coefficient: 0.883, P: 0.004) in the intestine. The expression level of type I IFN was also significantly up-regulated and the GCRV titer was significantly decreased (P < .05) in GCRV-infected ScIRF3-overexpressing Ctenopharyngodon idellus kidney cells. These results indicate that ScIRF3 may play a role in the type I IFN immune response against GCRV in Squaliobarbus curriculus and can also inhibit GCRV replication in Ctenopharyngodon idellus kidney cells.
[Mh] Termos MeSH primário: Cyprinidae/imunologia
Doenças dos Peixes/imunologia
Proteínas de Peixes/imunologia
Fator Regulador 3 de Interferon/imunologia
Reoviridae/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Cyprinidae/metabolismo
Cyprinidae/virologia
Doenças dos Peixes/metabolismo
Doenças dos Peixes/virologia
Proteínas de Peixes/classificação
Proteínas de Peixes/metabolismo
Perfilação da Expressão Gênica/métodos
Interações Hospedeiro-Patógeno/imunologia
Fator Regulador 3 de Interferon/genética
Fator Regulador 3 de Interferon/metabolismo
Interferon Tipo I/genética
Interferon Tipo I/imunologia
Interferon Tipo I/metabolismo
Filogenia
Reoviridae/fisiologia
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Interferon Regulatory Factor-3); 0 (Interferon Type I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:29331483
[Au] Autor:Liu X; Li Z; Wang B; Zhu H; Liu Y; Qi J; Zhang Q
[Ad] Endereço:Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, 266003 Qingdao, Shandong, China.
[Ti] Título:GATA4 is a transcriptional regulator of R-spondin1 in Japanese flounder (Paralichthys olivaceus).
[So] Source:Gene;648:68-75, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:GATA4 is a well-known transcription factor of the GATA family implicated in regulation of sex determination and gonadal development in mammals. In this study, we cloned the full-length cDNA of Paralichthys olivaceus gata4 (Po-gata4). Phylogenetic, gene structure, and synteny analysis showed that Po-GATA4 is homologous to GATA4 of teleost and tetrapod. Po-gata4 transcripts were detected in Sertoli cells, spermatogonia, oogonia and oocytes, with higher transcript levels overall in the testis than the ovary. The promoter region of P. olivaceus R-spondin1was found to contain a GATA4-binding motif. Results of CBA (cleaved amplified polymorphic sequence-based binding assay) indicated that GATA4 could indeed bind to the promoter sequence of R-spondin1. Moreover, human GATA4 recombinant protein could upregulate R-spondin1 in P. olivaceus ovary cells and FBCs (flounder brain cell line). In FBCs, overexpression of Po-gata4 resulted in elevated transcript levels of R-spondin1. Taken together, our results indicate that Po-GATA4 is involved in gonadal development by regulating R-spondin1 expression.
[Mh] Termos MeSH primário: Embrião não Mamífero/metabolismo
Proteínas de Peixes/genética
Linguado/genética
Fator de Transcrição GATA4/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Linhagem Celular
Embrião não Mamífero/citologia
Embrião não Mamífero/embriologia
Feminino
Proteínas de Peixes/classificação
Proteínas de Peixes/metabolismo
Linguado/embriologia
Linguado/metabolismo
Fator de Transcrição GATA4/classificação
Fator de Transcrição GATA4/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Masculino
Oogônios/citologia
Oogônios/metabolismo
Filogenia
Regiões Promotoras Genéticas/genética
Ligação Proteica
Espermatogônias/citologia
Espermatogônias/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (GATA4 Transcription Factor)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


  3 / 5812 MEDLINE  
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[PMID]:27777016
[Au] Autor:Zhang Y; Loughery JR; Martyniuk CJ; Kieffer JD
[Ad] Endereço:Department of Biological Sciences, University of New Brunswick, Saint John, New Brunswick, Canada; Department of Biological Science, University of Alberta, Edmonton, AB, Canada.
[Ti] Título:Physiological and molecular responses of juvenile shortnose sturgeon (Acipenser brevirostrum) to thermal stress.
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;203:314-321, 2017 01.
[Is] ISSN:1531-4332
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The shortnose sturgeon (Acipenser brevirostrum LeSueur, 1818) is a vulnerable species that is found along the eastern coast of North America. Little is known about temperature tolerance in this species and with a rapidly changing global climate, it becomes increasingly important to define the thermal tolerance of this species to better predict population distribution. Using a modified critical thermal maximum test (CTMax), the objectives of this study were to determine the impact of heating rate (0.1, 0.2 and 0.25°Cmin ) on the thermal tolerance, associated hematological responses, and oxygen consumption in juvenile sturgeon. In addition, transcripts associated with physiological stress and heat shock (i.e., heat shock proteins) were also measured. Heating rate did not alter the CTMax values of shortnose sturgeon. Neither heating rate nor thermal stress affected plasma sodium and chloride levels, nor the expression of transcripts that included catalase, glucocorticoid receptor, heat shock protein70 (hsp70), heat shock protein 90α (hsp90α) and cytochrome P450 1a (cyp1a). However, regardless of heating rate, thermal stress increased both plasma potassium and lactate concentrations. Glucose levels were increased at heating rates of 0.2 and 0.25°Cmin , but not at 0.1°Cmin . Overall, oxygen consumption rates increased with thermal stress, but the response patterns were not affected by heating rate. These data support the hypothesis that shortnose sturgeon can tolerate acute heat stress, as many physiological and molecular parameters measured here were non-responsive to the thermal stress.
[Mh] Termos MeSH primário: Glicemia/análise
Peixes/fisiologia
Ácido Láctico/sangue
Potássio/sangue
Estresse Fisiológico
Termotolerância
Regulação para Cima
[Mh] Termos MeSH secundário: Algoritmos
Animais
Aquicultura
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Peixes/sangue
Peixes/crescimento & desenvolvimento
Regulação da Expressão Gênica no Desenvolvimento
Aquecimento Global
Cinética
Novo Brunswick
Consumo de Oxigênio
Distribuição Aleatória
Rios
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Fish Proteins); 33X04XA5AT (Lactic Acid); RWP5GA015D (Potassium)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  4 / 5812 MEDLINE  
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[PMID]:29372967
[Au] Autor:Pankova MV; Kukhlevsky AD; Brykov VA
[Ti] Título:[Fish growth hormone genes: Divergence of coding sequences in salmonid fishes].
[So] Source:Genetika;53(2):201-13, 2017 Feb.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Comparison of coding nucleotide sequences of the paralogous GH1 and GH2 genes, as well as of the growth hormone amino acid sequences, in the species of closely related salmonid genera Salvelinus, Oncorhynchus, and Salmo was performed. It was demonstrated that, in different groups of salmonids, the amino acid substitution rates were considerably different. In some cases, an obvious discrepancy between the divergence of growth hormone genes and phylogenetic schemes based on other methods and approaches was revealed. These findings suggest that the reason may be multidirectional selection at duplicated genes at different stages of evolution.
[Mh] Termos MeSH primário: Evolução Molecular
Proteínas de Peixes/genética
Hormônio do Crescimento/genética
Filogenia
Salmonidae/genética
[Mh] Termos MeSH secundário: Animais
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 9002-72-6 (Growth Hormone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


  5 / 5812 MEDLINE  
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[PMID]:29372806
[Au] Autor:Slynko YV; Slynko EE; Karpova EP; Boltachev AR
[Ti] Título:[Genetic variation of the mtDNA cyt b locus in topmouth gudgeon introduced into water bodies in the northern part of the Black Sea region].
[So] Source:Genetika;53(1):79-87, 2017 Jan.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The up-to-date phylogeographical distribution of the topmouth gudgeon Pseudorasbora parva Temminck et Schlegel 1846 in water bodies of the Northern Black Sea region is considered. Genetic variation of mtDNA cyt b gene is analyzed. It is established that topmouth gudgeon penetrated and spread in the basins of the Dnieper and Don rivers and in water bodies of Crimea from the secondary center of its dispersion­ water bodies of Central Europe. It is demonstrated that haplotypes of topmouth gudgeon in the Danube delta are the most homologous to the haplotypes in the native range of the species in China. A considerable decrease in the level of genetic variation in the populations in the Black Sea region is reported.
[Mh] Termos MeSH primário: Cyprinidae/genética
Citocromos b/genética
DNA Mitocondrial/genética
Proteínas de Peixes/genética
Variação Genética
Proteínas Mitocondriais/genética
[Mh] Termos MeSH secundário: Animais
Mar Negro
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Fish Proteins); 0 (Mitochondrial Proteins); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


  6 / 5812 MEDLINE  
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[PMID]:29262703
[Au] Autor:Gyori J; Farkas A; Stolyar O; Székács A; Mörtl M; Vehovszky Á
[Ad] Endereço:1 Department of Experimental Zoology, MTA Centre for Ecological Research, Balaton Limnological Institute , H-8237 Tihany, POB 35 , Hungary.
[Ti] Título:Inhibitory effects of four neonicotinoid active ingredients on acetylcholine esterase activity.
[So] Source:Acta Biol Hung;68(4):345-357, 2017 Dec.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:There is a great concern about the decline of pollinators, and neonicotinoids emerging bee disorders are assumed to play a significant role. Since changes in learning ability has been observed in honey bees exposed to some acetylcholine esterase (AChE) inhibitors, we therefore, tested in vitro the effect of four neonicotinoids on purified eel AChE. AChE activity was inhibited in a concentration-dependent manner, and calculated IC values for thiamethoxam (IC = 414 µM) and clothianidin (IC = 160 µM) were found to be much higher compared to acetamiprid (IC = 75.2 µM) and thiacloprid (IC = 87.8 µM). The Lineweaver-Burk reciprocal plots for acetamiprid shows unchanged V and increased K values with inhibitor concentrations, while analysis of Michaelis-Menten plots shows predominantly competitive mechanism. The inhibition constant value (K = 24.3 µM) indicates strong binding of the acetamiprid complex to AChE. Finally, the four tested neonicotinoids are not a uniform group regarding their blocking ability. Our results suggest a previously not established, direct AChE blocking mechanism of neonicotinoids tested, thus the neuronal AChE enzyme is likely among the direct targets of the neonicotinoid insecticides. We conclude, that these AChE inhibitory effects may also contribute to toxic effects on the whole exposed animal.
[Mh] Termos MeSH primário: Acetilcolinesterase/química
Inibidores da Colinesterase/química
Electrophorus
Proteínas de Peixes
Guanidinas/química
Neonicotinoides/química
Nitrocompostos/química
Oxazinas/química
Tiazóis/química
[Mh] Termos MeSH secundário: Animais
Proteínas de Peixes/antagonistas & inibidores
Proteínas de Peixes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Fish Proteins); 0 (Guanidines); 0 (Neonicotinoids); 0 (Nitro Compounds); 0 (Oxazines); 0 (Thiazoles); 2V9906ABKQ (clothianidin); 5HL5N372P0 (acetamiprid); 747IC8B487 (thiamethoxam); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.4.1


  7 / 5812 MEDLINE  
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[PMID]:29225165
[Au] Autor:Chakraborty A; Uechi T; Nakajima Y; Gazda HT; O'Donohue MF; Gleizes PE; Kenmochi N
[Ad] Endereço:Division of Molecular Genetics and Cancer, NU Centre for Science Education & Research, Nitte University, Mangalore 18, India. Electronic address: anirban@nitte.edu.in.
[Ti] Título:Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in vivo and in vitro models.
[So] Source:Biochem Biophys Res Commun;495(2):1839-1845, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mutations in genes encoding ribosomal proteins have been identified in Diamond-Blackfan anemia (DBA), a rare genetic disorder that presents with a prominent erythroid phenotype. TP53 has been implicated in the pathophysiology of DBA with ribosomal protein (RP) L11 playing a crucial role in the TP53 response. Interestingly, RPL11 also controls the transcriptional activity of c-Myc, an oncoprotein that positively regulates ribosome biogenesis. In the present study, we analyzed the consequences of rpl11 depletion on erythropoiesis and ribosome biogenesis in zebrafish. As expected, Rpl11-deficient zebrafish exhibited defects in ribosome biogenesis and an anemia phenotype. However, co-inhibition of Tp53 did not alleviate the erythroid aplasia in these fish. Next, we explored the role of c-Myc in RPL11-deficient cellular and animal models. c-Myc and its target nucleolar proteins showed upregulation and increased localization in the head region of Rpl11-deficient zebrafish, where the morphological abnormalities and tp53 expression were more pronounced. Interestingly, in blood cells derived from DBA patients with mutations in RPL11, the biogenesis of ribosomes was defective, but the expression level of c-Myc and its target nucleolar proteins was unchanged. The results suggest a model whereby RPL11 deficiency activates the synthesis of c-Myc target nucleolar proteins, which subsequently triggers a p53 response. These results further demonstrate that the induction of Tp53 mediates the morphological, but not erythroid, defects associated with RPL11 deficiency.
[Mh] Termos MeSH primário: Anemia de Diamond-Blackfan/fisiopatologia
Proteínas Ribossômicas/deficiência
[Mh] Termos MeSH secundário: Anemia de Diamond-Blackfan/genética
Anemia de Diamond-Blackfan/patologia
Animais
Modelos Animais de Doenças
Eritropoese/genética
Proteínas de Peixes/deficiência
Proteínas de Peixes/genética
Genes myc
Genes p53
Seres Humanos
Mutação
Processamento Pós-Transcricional do RNA
Proteínas Ribossômicas/genética
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Ribosomal Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE


  8 / 5812 MEDLINE  
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[PMID]:29198705
[Au] Autor:Zeng J; Li Z; Lui EY; Lam SH; Swaminathan K
[Ad] Endereço:Department of Biological Sciences, National University of Singapore 117543, Singapore.
[Ti] Título:Tilapia and human CLIC2 structures are highly conserved.
[So] Source:Biochem Biophys Res Commun;495(2):1752-1757, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chloride intracellular channels (CLICs) exist in soluble and membrane bound forms. We have determined the crystal structure of soluble Clic2 from the euryhaline teleost fish Oreochromis mossambicus. Structural comparison of tilapia and human CLIC2 with other CLICs shows that these proteins are highly conserved. We have also compared the expression levels of clic2 in selected osmoregulatory organs of tilapia, acclimated to freshwater, seawater and hypersaline water. Structural conservation of vertebrate CLICs implies that they might play conserved roles. Also, tissue-specific responsiveness of clic2 suggests that it might be involved in iono-osmoregulation under extreme conditions in tilapia.
[Mh] Termos MeSH primário: Canais de Cloreto/química
Canais de Cloreto/genética
Proteínas de Peixes/química
Proteínas de Peixes/genética
Tilápia/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Canais de Cloreto/metabolismo
Sequência Conservada
Proteínas de Peixes/metabolismo
Seres Humanos
Modelos Moleculares
Osmorregulação/genética
Osmorregulação/fisiologia
Filogenia
Conformação Proteica
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Salinidade
Homologia de Sequência de Aminoácidos
Tilápia/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CLIC2 protein, human); 0 (Chloride Channels); 0 (Fish Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


  9 / 5812 MEDLINE  
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[PMID]:29329928
[Au] Autor:Dong Y; Zhao J; Chen J; Wang S; Liu Y; Zhang Q; You C; Monroig Ó; Tocher DR; Li Y
[Ad] Endereço:School of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
[Ti] Título:Cloning and characterization of ∆6/∆5 fatty acyl desaturase (Fad) gene promoter in the marine teleost Siganus canaliculatus.
[So] Source:Gene;647:174-180, 2018 Mar 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The rabbitfish Siganus canaliculatus was the first marine teleost demonstrated to have the ability of biosynthesizing long-chain polyunsaturated fatty acids (LC-PUFA) from C PUFA precursors, and all genes encoding the key enzymes for LC-PUFA biosynthesis have been cloned and functionally characterized, which provides us a potential model to study the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. As the primary step to clarify such mechanisms, present research focused on promoter analysis of gene encoding ∆6/∆5 fatty acyl desaturase (Fad), a rate-limiting enzyme catalyzing the first step in the conversion of C PUFA to LC-PUFA. First, 2044 bp promoter sequence was cloned by genome walking, and the sequence from -456 bp to +51 bp was determined as core promoter by progressive deletion mutation. Moreover, binding sites of transcription factors (TF) such as CCAAT enhancer binding protein (C/EBP), nuclear factor 1 (NF-1), stimulatory protein 1 (Sp1), nuclear factor Y (NF-Y), activated protein 1 (AP1), sterol regulatory element (SRE), hepatocyte nuclear factor 4α (HNF4α) and peroxisome proliferator activated receptor γ (PPARγ) were identified in the core promoter by site-directed mutation and functional assays. Moreover, NF-1 and HNF4α were confirmed to interact with the core promoter region by gel shift assay and mass spectrometry. This is the first report of the promoter structure of a ∆6/∆5 Fad in a marine teleost, and a novel discovery of NF-1 and HNF4α binding to the ∆6/∆5 Fad promoter.
[Mh] Termos MeSH primário: Ácidos Graxos Insaturados/genética
Peixes/genética
Regiões Promotoras Genéticas/genética
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação/genética
Fator de Ligação a CCAAT/genética
Clonagem Molecular/métodos
Proteínas de Peixes
Fator de Transcrição Sp1/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCAAT-Binding Factor); 0 (Fatty Acids, Unsaturated); 0 (Fish Proteins); 0 (Sp1 Transcription Factor); 0 (nuclear factor Y)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180114
[St] Status:MEDLINE


  10 / 5812 MEDLINE  
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[PMID]:27776996
[Au] Autor:Holm HJ; Skugor S; Bjelland AK; Radunovic S; Wadsworth S; Koppang EO; Evensen Ø
[Ad] Endereço:Faculty of Veterinary Medicine and Biosciences, Department of Basic Sciences and Aquatic Medicine, Sea Lice Research Centre, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: Helle.Holm@nmbu.no.
[Ti] Título:Contrasting expression of immune genes in scaled and scaleless skin of Atlantic salmon infected with young stages of Lepeophtheirus salmonis.
[So] Source:Dev Comp Immunol;67:153-165, 2017 02.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Atlantic salmon skin tissues with and without scales were taken from two preferred sites of salmon louse (Lepeophtheirus salmonis) attachment, behind the dorsal fin (scaled) and from the top of the head (scaleless), respectively. Tissues were profiled by qPCR of 32 genes to study responses to copepodids, 4 days post infection (dpi), and during the moult of copepodids to the chalimus stage, at 8 dpi. Basal/constitutive differences were found for many immune-related genes between the two skin sites; e.g., mannose binding protein C was over 100 fold higher expressed in the scaled skin from the back in comparison to the skin without scales from the head. With lice-infection, at 4 dpi most genes in both tissues showed lower values than in the non-infected control. By 8 dpi, the majority of responses increased towards the control levels, including cytokines of Th1, Th17 and Th2 pathways. Immunohistochemistry of three immune factors revealed an even distribution of MHC class II positive cells throughout epidermis, including the top layer of keratinocytes, marked compartmentalization of Mx and CD8α cells close to stratum basale, and an increase in numbers of CD8α cells in response to infection. In conclusion, suppression of immune genes during the copepodid stage likely sets off a beneficial situation for the parasite. At the moult to chalimus stage 8 dpi, only few genes surpassed the non-infected control levels, including CD8α. The gene expression pattern was reflected in the increased number of CD8α expressing cells, thus revealing a relatively minor activation of skin T-cell defenses in Atlantic salmon in response to L. salmonis infection.
[Mh] Termos MeSH primário: Escamas de Animais/fisiologia
Copépodes/imunologia
Proteínas de Peixes/metabolismo
Infestações por Piolhos/imunologia
Lectina de Ligação a Manose/metabolismo
Salmo salar/imunologia
Pele/imunologia
[Mh] Termos MeSH secundário: Escamas de Animais/parasitologia
Animais
Células Cultivadas
Citocinas/metabolismo
Proteínas de Peixes/genética
Imunidade/genética
Infestações por Piolhos/genética
Estágios do Ciclo de Vida
Lectina de Ligação a Manose/genética
Salmo salar/parasitologia
Pele/parasitologia
Fenômenos Fisiológicos da Pele/genética
Células Th1/imunologia
Células Th17/imunologia
Células Th2/imunologia
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Fish Proteins); 0 (MBL2 protein, human); 0 (Mannose-Binding Lectin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE



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