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[PMID]: 29339070 [Au] Autor: Wang R; Li Y; Zhou Z; Liu Q; Zeng L; Xiao T [Ad] Endereço: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China; College of Life and Environment Sciences, Hunan University of Arts and Science, Changde, Hunan 415000, China. [Ti] Título: Involvement of interferon regulatory factor 3 from the barbel chub Squaliobarbus curriculus in the immune response against grass carp reovirus. [So] Source: Gene;648:5-11, 2018 Mar 30. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The barbel chub Squaliobarbus curriculus is an important commercial fish species in China, and has shown significant resistance to grass carp reovirus (GCRV). In this study, the cDNA sequence of interferon regulatory factors 3 (IRF3) from Squaliobarbus curriculus, designated as ScIRF3, was cloned, and its effect against GCRV was investigated. The full-length 1837 base pair (bp) cDNA of ScIRF3 contained a complete open reading frame of 1374 bp and encoded a putative polypeptide of 457 amino acid residues. The ScIRF3 protein contained conserved domains, including an N-terminal DNA-binding domain, a C-terminal IRF association domain, and a serine-rich domain. Phylogenetic analysis showed that ScIRF3 was closely clustered with IRF3s from Carassius auratus and Ctenopharyngodon idellus. Quantitative real-time polymerase chain reaction analysis showed that the expression levels of ScIRF3 in Squaliobarbus curriculus were the highest in the spleen and lowest in the muscle. After GCRV infection, expression levels of both ScIRF3 and type I interferon (IFN) were initially up-regulated and subsequently down-regulated in the spleen and intestine. Correlation analysis showed that the expression level of type I IFN is significantly positively correlated with that of ScIRF3 (Pearson correlation coefficient: 0.883, P: 0.004) in the intestine. The expression level of type I IFN was also significantly up-regulated and the GCRV titer was significantly decreased (P < .05) in GCRV-infected ScIRF3-overexpressing Ctenopharyngodon idellus kidney cells. These results indicate that ScIRF3 may play a role in the type I IFN immune response against GCRV in Squaliobarbus curriculus and can also inhibit GCRV replication in Ctenopharyngodon idellus kidney cells. [Mh] Termos MeSH primário: Cyprinidae/imunologia Doenças dos Peixes/imunologia Proteínas de Peixes/imunologia Fator Regulador 3 de Interferon/imunologia Reoviridae/imunologia [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Cyprinidae/metabolismo Cyprinidae/virologia Doenças dos Peixes/metabolismo Doenças dos Peixes/virologia Proteínas de Peixes/classificação Proteínas de Peixes/metabolismo Perfilação da Expressão Gênica/métodos Interações Hospedeiro-Patógeno/imunologia Fator Regulador 3 de Interferon/genética Fator Regulador 3 de Interferon/metabolismo Interferon Tipo I/genética Interferon Tipo I/imunologia Interferon Tipo I/metabolismo Filogenia Reoviridae/fisiologia Homologia de Sequência de Aminoácidos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fish Proteins); 0 (Interferon Regulatory Factor-3); 0 (Interferon Type I) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180226 [Lr] Data última revisão: 180226 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180118 [St] Status: MEDLINE

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[PMID]: 29331483 [Au] Autor: Liu X; Li Z; Wang B; Zhu H; Liu Y; Qi J; Zhang Q [Ad] Endereço: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, 266003 Qingdao, Shandong, China. [Ti] Título: GATA4 is a transcriptional regulator of R-spondin1 in Japanese flounder (Paralichthys olivaceus). [So] Source: Gene;648:68-75, 2018 Mar 30. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: GATA4 is a well-known transcription factor of the GATA family implicated in regulation of sex determination and gonadal development in mammals. In this study, we cloned the full-length cDNA of Paralichthys olivaceus gata4 (Po-gata4). Phylogenetic, gene structure, and synteny analysis showed that Po-GATA4 is homologous to GATA4 of teleost and tetrapod. Po-gata4 transcripts were detected in Sertoli cells, spermatogonia, oogonia and oocytes, with higher transcript levels overall in the testis than the ovary. The promoter region of P. olivaceus R-spondin1was found to contain a GATA4-binding motif. Results of CBA (cleaved amplified polymorphic sequence-based binding assay) indicated that GATA4 could indeed bind to the promoter sequence of R-spondin1. Moreover, human GATA4 recombinant protein could upregulate R-spondin1 in P. olivaceus ovary cells and FBCs (flounder brain cell line). In FBCs, overexpression of Po-gata4 resulted in elevated transcript levels of R-spondin1. Taken together, our results indicate that Po-GATA4 is involved in gonadal development by regulating R-spondin1 expression. [Mh] Termos MeSH primário: Embrião não Mamífero/metabolismo Proteínas de Peixes/genética Linguado/genética Fator de Transcrição GATA4/genética [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Sequência de Bases Linhagem Celular Embrião não Mamífero/citologia Embrião não Mamífero/embriologia Feminino Proteínas de Peixes/classificação Proteínas de Peixes/metabolismo Linguado/embriologia Linguado/metabolismo Fator de Transcrição GATA4/classificação Fator de Transcrição GATA4/metabolismo Regulação da Expressão Gênica no Desenvolvimento Masculino Oogônios/citologia Oogônios/metabolismo Filogenia Regiões Promotoras Genéticas/genética Ligação Proteica Espermatogônias/citologia Espermatogônias/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fish Proteins); 0 (GATA4 Transcription Factor) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180226 [Lr] Data última revisão: 180226 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180115 [St] Status: MEDLINE

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[PMID]: 27777016 [Au] Autor: Zhang Y; Loughery JR; Martyniuk CJ; Kieffer JD [Ad] Endereço: Department of Biological Sciences, University of New Brunswick, Saint John, New Brunswick, Canada; Department of Biological Science, University of Alberta, Edmonton, AB, Canada. [Ti] Título: Physiological and molecular responses of juvenile shortnose sturgeon (Acipenser brevirostrum) to thermal stress. [So] Source: Comp Biochem Physiol A Mol Integr Physiol;203:314-321, 2017 01. [Is] ISSN: 1531-4332 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The shortnose sturgeon (Acipenser brevirostrum LeSueur, 1818) is a vulnerable species that is found along the eastern coast of North America. Little is known about temperature tolerance in this species and with a rapidly changing global climate, it becomes increasingly important to define the thermal tolerance of this species to better predict population distribution. Using a modified critical thermal maximum test (CTMax), the objectives of this study were to determine the impact of heating rate (0.1, 0.2 and 0.25°Cmin ) on the thermal tolerance, associated hematological responses, and oxygen consumption in juvenile sturgeon. In addition, transcripts associated with physiological stress and heat shock (i.e., heat shock proteins) were also measured. Heating rate did not alter the CTMax values of shortnose sturgeon. Neither heating rate nor thermal stress affected plasma sodium and chloride levels, nor the expression of transcripts that included catalase, glucocorticoid receptor, heat shock protein70 (hsp70), heat shock protein 90α (hsp90α) and cytochrome P450 1a (cyp1a). However, regardless of heating rate, thermal stress increased both plasma potassium and lactate concentrations. Glucose levels were increased at heating rates of 0.2 and 0.25°Cmin , but not at 0.1°Cmin . Overall, oxygen consumption rates increased with thermal stress, but the response patterns were not affected by heating rate. These data support the hypothesis that shortnose sturgeon can tolerate acute heat stress, as many physiological and molecular parameters measured here were non-responsive to the thermal stress. [Mh] Termos MeSH primário: Glicemia/análise Peixes/fisiologia Ácido Láctico/sangue Potássio/sangue Estresse Fisiológico Termotolerância Regulação para Cima [Mh] Termos MeSH secundário: Algoritmos Animais Aquicultura Proteínas de Peixes/genética Proteínas de Peixes/metabolismo Peixes/sangue Peixes/crescimento & desenvolvimento Regulação da Expressão Gênica no Desenvolvimento Aquecimento Global Cinética Novo Brunswick Consumo de Oxigênio Distribuição Aleatória Rios [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Blood Glucose); 0 (Fish Proteins); 33X04XA5AT (Lactic Acid); RWP5GA015D (Potassium) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 180220 [Lr] Data última revisão: 180220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161107 [St] Status: MEDLINE

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[PMID]: 29372967 [Au] Autor: Pankova MV; Kukhlevsky AD; Brykov VA [Ti] Título: [Fish growth hormone genes: Divergence of coding sequences in salmonid fishes]. [So] Source: Genetika;53(2):201-13, 2017 Feb. [Is] ISSN: 0016-6758 [Cp] País de publicação: Russia (Federation) [La] Idioma: rus [Ab] Resumo: Comparison of coding nucleotide sequences of the paralogous GH1 and GH2 genes, as well as of the growth hormone amino acid sequences, in the species of closely related salmonid genera Salvelinus, Oncorhynchus, and Salmo was performed. It was demonstrated that, in different groups of salmonids, the amino acid substitution rates were considerably different. In some cases, an obvious discrepancy between the divergence of growth hormone genes and phylogenetic schemes based on other methods and approaches was revealed. These findings suggest that the reason may be multidirectional selection at duplicated genes at different stages of evolution. [Mh] Termos MeSH primário: Evolução Molecular Proteínas de Peixes/genética Hormônio do Crescimento/genética Filogenia Salmonidae/genética [Mh] Termos MeSH secundário: Animais Especificidade da Espécie [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fish Proteins); 9002-72-6 (Growth Hormone) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180216 [Lr] Data última revisão: 180216 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180127 [St] Status: MEDLINE

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[PMID]: 29372806 [Au] Autor: Slynko YV; Slynko EE; Karpova EP; Boltachev AR [Ti] Título: [Genetic variation of the mtDNA cyt b locus in topmouth gudgeon introduced into water bodies in the northern part of the Black Sea region]. [So] Source: Genetika;53(1):79-87, 2017 Jan. [Is] ISSN: 0016-6758 [Cp] País de publicação: Russia (Federation) [La] Idioma: rus [Ab] Resumo: The up-to-date phylogeographical distribution of the topmouth gudgeon Pseudorasbora parva Temminck et Schlegel 1846 in water bodies of the Northern Black Sea region is considered. Genetic variation of mtDNA cyt b gene is analyzed. It is established that topmouth gudgeon penetrated and spread in the basins of the Dnieper and Don rivers and in water bodies of Crimea from the secondary center of its dispersion­ water bodies of Central Europe. It is demonstrated that haplotypes of topmouth gudgeon in the Danube delta are the most homologous to the haplotypes in the native range of the species in China. A considerable decrease in the level of genetic variation in the populations in the Black Sea region is reported. [Mh] Termos MeSH primário: Cyprinidae/genética Citocromos b/genética DNA Mitocondrial/genética Proteínas de Peixes/genética Variação Genética Proteínas Mitocondriais/genética [Mh] Termos MeSH secundário: Animais Mar Negro [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Mitochondrial); 0 (Fish Proteins); 0 (Mitochondrial Proteins); 9035-37-4 (Cytochromes b) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180216 [Lr] Data última revisão: 180216 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180127 [St] Status: MEDLINE

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[PMID]: 29262703 [Au] Autor: Gyori J; Farkas A; Stolyar O; Székács A; Mörtl M; Vehovszky Á [Ad] Endereço: 1 Department of Experimental Zoology, MTA Centre for Ecological Research, Balaton Limnological Institute , H-8237 Tihany, POB 35 , Hungary. [Ti] Título: Inhibitory effects of four neonicotinoid active ingredients on acetylcholine esterase activity. [So] Source: Acta Biol Hung;68(4):345-357, 2017 Dec. [Is] ISSN: 0236-5383 [Cp] País de publicação: Hungary [La] Idioma: eng [Ab] Resumo: There is a great concern about the decline of pollinators, and neonicotinoids emerging bee disorders are assumed to play a significant role. Since changes in learning ability has been observed in honey bees exposed to some acetylcholine esterase (AChE) inhibitors, we therefore, tested in vitro the effect of four neonicotinoids on purified eel AChE. AChE activity was inhibited in a concentration-dependent manner, and calculated IC values for thiamethoxam (IC = 414 µM) and clothianidin (IC = 160 µM) were found to be much higher compared to acetamiprid (IC = 75.2 µM) and thiacloprid (IC = 87.8 µM). The Lineweaver-Burk reciprocal plots for acetamiprid shows unchanged V and increased K values with inhibitor concentrations, while analysis of Michaelis-Menten plots shows predominantly competitive mechanism. The inhibition constant value (K = 24.3 µM) indicates strong binding of the acetamiprid complex to AChE. Finally, the four tested neonicotinoids are not a uniform group regarding their blocking ability. Our results suggest a previously not established, direct AChE blocking mechanism of neonicotinoids tested, thus the neuronal AChE enzyme is likely among the direct targets of the neonicotinoid insecticides. We conclude, that these AChE inhibitory effects may also contribute to toxic effects on the whole exposed animal. [Mh] Termos MeSH primário: Acetilcolinesterase/química Inibidores da Colinesterase/química Electrophorus Proteínas de Peixes Guanidinas/química Neonicotinoides/química Nitrocompostos/química Oxazinas/química Tiazóis/química [Mh] Termos MeSH secundário: Animais Proteínas de Peixes/antagonistas & inibidores Proteínas de Peixes/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cholinesterase Inhibitors); 0 (Fish Proteins); 0 (Guanidines); 0 (Neonicotinoids); 0 (Nitro Compounds); 0 (Oxazines); 0 (Thiazoles); 2V9906ABKQ (clothianidin); 5HL5N372P0 (acetamiprid); 747IC8B487 (thiamethoxam); EC 3.1.1.7 (Acetylcholinesterase) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180216 [Lr] Data última revisão: 180216 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171222 [St] Status: MEDLINE [do] DOI: 10.1556/018.68.2017.4.1

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[PMID]: 29225165 [Au] Autor: Chakraborty A; Uechi T; Nakajima Y; Gazda HT; O'Donohue MF; Gleizes PE; Kenmochi N [Ad] Endereço: Division of Molecular Genetics and Cancer, NU Centre for Science Education & Research, Nitte University, Mangalore 18, India. Electronic address: anirban@nitte.edu.in. [Ti] Título: Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in vivo and in vitro models. [So] Source: Biochem Biophys Res Commun;495(2):1839-1845, 2018 01 08. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Mutations in genes encoding ribosomal proteins have been identified in Diamond-Blackfan anemia (DBA), a rare genetic disorder that presents with a prominent erythroid phenotype. TP53 has been implicated in the pathophysiology of DBA with ribosomal protein (RP) L11 playing a crucial role in the TP53 response. Interestingly, RPL11 also controls the transcriptional activity of c-Myc, an oncoprotein that positively regulates ribosome biogenesis. In the present study, we analyzed the consequences of rpl11 depletion on erythropoiesis and ribosome biogenesis in zebrafish. As expected, Rpl11-deficient zebrafish exhibited defects in ribosome biogenesis and an anemia phenotype. However, co-inhibition of Tp53 did not alleviate the erythroid aplasia in these fish. Next, we explored the role of c-Myc in RPL11-deficient cellular and animal models. c-Myc and its target nucleolar proteins showed upregulation and increased localization in the head region of Rpl11-deficient zebrafish, where the morphological abnormalities and tp53 expression were more pronounced. Interestingly, in blood cells derived from DBA patients with mutations in RPL11, the biogenesis of ribosomes was defective, but the expression level of c-Myc and its target nucleolar proteins was unchanged. The results suggest a model whereby RPL11 deficiency activates the synthesis of c-Myc target nucleolar proteins, which subsequently triggers a p53 response. These results further demonstrate that the induction of Tp53 mediates the morphological, but not erythroid, defects associated with RPL11 deficiency. [Mh] Termos MeSH primário: Anemia de Diamond-Blackfan/fisiopatologia Proteínas Ribossômicas/deficiência [Mh] Termos MeSH secundário: Anemia de Diamond-Blackfan/genética Anemia de Diamond-Blackfan/patologia Animais Modelos Animais de Doenças Eritropoese/genética Proteínas de Peixes/deficiência Proteínas de Peixes/genética Genes myc Genes p53 Seres Humanos Mutação Processamento Pós-Transcricional do RNA Proteínas Ribossômicas/genética Peixe-Zebra [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Fish Proteins); 0 (Ribosomal Proteins) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180212 [Lr] Data última revisão: 180212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171212 [St] Status: MEDLINE

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[PMID]: 29198705 [Au] Autor: Zeng J; Li Z; Lui EY; Lam SH; Swaminathan K [Ad] Endereço: Department of Biological Sciences, National University of Singapore 117543, Singapore. [Ti] Título: Tilapia and human CLIC2 structures are highly conserved. [So] Source: Biochem Biophys Res Commun;495(2):1752-1757, 2018 01 08. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Chloride intracellular channels (CLICs) exist in soluble and membrane bound forms. We have determined the crystal structure of soluble Clic2 from the euryhaline teleost fish Oreochromis mossambicus. Structural comparison of tilapia and human CLIC2 with other CLICs shows that these proteins are highly conserved. We have also compared the expression levels of clic2 in selected osmoregulatory organs of tilapia, acclimated to freshwater, seawater and hypersaline water. Structural conservation of vertebrate CLICs implies that they might play conserved roles. Also, tissue-specific responsiveness of clic2 suggests that it might be involved in iono-osmoregulation under extreme conditions in tilapia. [Mh] Termos MeSH primário: Canais de Cloreto/química Canais de Cloreto/genética Proteínas de Peixes/química Proteínas de Peixes/genética Tilápia/genética [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Canais de Cloreto/metabolismo Sequência Conservada Proteínas de Peixes/metabolismo Seres Humanos Modelos Moleculares Osmorregulação/genética Osmorregulação/fisiologia Filogenia Conformação Proteica RNA Mensageiro/genética RNA Mensageiro/metabolismo Salinidade Homologia de Sequência de Aminoácidos Tilápia/fisiologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (CLIC2 protein, human); 0 (Chloride Channels); 0 (Fish Proteins); 0 (RNA, Messenger) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180212 [Lr] Data última revisão: 180212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171205 [St] Status: MEDLINE

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[PMID]: 29329928 [Au] Autor: Dong Y; Zhao J; Chen J; Wang S; Liu Y; Zhang Q; You C; Monroig Ó; Tocher DR; Li Y [Ad] Endereço: School of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China. [Ti] Título: Cloning and characterization of ∆6/∆5 fatty acyl desaturase (Fad) gene promoter in the marine teleost Siganus canaliculatus. [So] Source: Gene;647:174-180, 2018 Mar 20. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The rabbitfish Siganus canaliculatus was the first marine teleost demonstrated to have the ability of biosynthesizing long-chain polyunsaturated fatty acids (LC-PUFA) from C PUFA precursors, and all genes encoding the key enzymes for LC-PUFA biosynthesis have been cloned and functionally characterized, which provides us a potential model to study the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. As the primary step to clarify such mechanisms, present research focused on promoter analysis of gene encoding ∆6/∆5 fatty acyl desaturase (Fad), a rate-limiting enzyme catalyzing the first step in the conversion of C PUFA to LC-PUFA. First, 2044 bp promoter sequence was cloned by genome walking, and the sequence from -456 bp to +51 bp was determined as core promoter by progressive deletion mutation. Moreover, binding sites of transcription factors (TF) such as CCAAT enhancer binding protein (C/EBP), nuclear factor 1 (NF-1), stimulatory protein 1 (Sp1), nuclear factor Y (NF-Y), activated protein 1 (AP1), sterol regulatory element (SRE), hepatocyte nuclear factor 4α (HNF4α) and peroxisome proliferator activated receptor γ (PPARγ) were identified in the core promoter by site-directed mutation and functional assays. Moreover, NF-1 and HNF4α were confirmed to interact with the core promoter region by gel shift assay and mass spectrometry. This is the first report of the promoter structure of a ∆6/∆5 Fad in a marine teleost, and a novel discovery of NF-1 and HNF4α binding to the ∆6/∆5 Fad promoter. [Mh] Termos MeSH primário: Ácidos Graxos Insaturados/genética Peixes/genética Regiões Promotoras Genéticas/genética [Mh] Termos MeSH secundário: Animais Sítios de Ligação/genética Fator de Ligação a CCAAT/genética Clonagem Molecular/métodos Proteínas de Peixes Fator de Transcrição Sp1/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (CCAAT-Binding Factor); 0 (Fatty Acids, Unsaturated); 0 (Fish Proteins); 0 (Sp1 Transcription Factor); 0 (nuclear factor Y) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180209 [Lr] Data última revisão: 180209 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180114 [St] Status: MEDLINE

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[PMID]: 27776996 [Au] Autor: Holm HJ; Skugor S; Bjelland AK; Radunovic S; Wadsworth S; Koppang EO; Evensen Ø [Ad] Endereço: Faculty of Veterinary Medicine and Biosciences, Department of Basic Sciences and Aquatic Medicine, Sea Lice Research Centre, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: Helle.Holm@nmbu.no. [Ti] Título: Contrasting expression of immune genes in scaled and scaleless skin of Atlantic salmon infected with young stages of Lepeophtheirus salmonis. [So] Source: Dev Comp Immunol;67:153-165, 2017 02. [Is] ISSN: 1879-0089 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Atlantic salmon skin tissues with and without scales were taken from two preferred sites of salmon louse (Lepeophtheirus salmonis) attachment, behind the dorsal fin (scaled) and from the top of the head (scaleless), respectively. Tissues were profiled by qPCR of 32 genes to study responses to copepodids, 4 days post infection (dpi), and during the moult of copepodids to the chalimus stage, at 8 dpi. Basal/constitutive differences were found for many immune-related genes between the two skin sites; e.g., mannose binding protein C was over 100 fold higher expressed in the scaled skin from the back in comparison to the skin without scales from the head. With lice-infection, at 4 dpi most genes in both tissues showed lower values than in the non-infected control. By 8 dpi, the majority of responses increased towards the control levels, including cytokines of Th1, Th17 and Th2 pathways. Immunohistochemistry of three immune factors revealed an even distribution of MHC class II positive cells throughout epidermis, including the top layer of keratinocytes, marked compartmentalization of Mx and CD8α cells close to stratum basale, and an increase in numbers of CD8α cells in response to infection. In conclusion, suppression of immune genes during the copepodid stage likely sets off a beneficial situation for the parasite. At the moult to chalimus stage 8 dpi, only few genes surpassed the non-infected control levels, including CD8α. The gene expression pattern was reflected in the increased number of CD8α expressing cells, thus revealing a relatively minor activation of skin T-cell defenses in Atlantic salmon in response to L. salmonis infection. [Mh] Termos MeSH primário: Escamas de Animais/fisiologia Copépodes/imunologia Proteínas de Peixes/metabolismo Infestações por Piolhos/imunologia Lectina de Ligação a Manose/metabolismo Salmo salar/imunologia Pele/imunologia [Mh] Termos MeSH secundário: Escamas de Animais/parasitologia Animais Células Cultivadas Citocinas/metabolismo Proteínas de Peixes/genética Imunidade/genética Infestações por Piolhos/genética Estágios do Ciclo de Vida Lectina de Ligação a Manose/genética Salmo salar/parasitologia Pele/parasitologia Fenômenos Fisiológicos da Pele/genética Células Th1/imunologia Células Th17/imunologia Células Th2/imunologia Transcriptoma [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Cytokines); 0 (Fish Proteins); 0 (MBL2 protein, human); 0 (Mannose-Binding Lectin) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 180210 [Lr] Data última revisão: 180210 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161026 [St] Status: MEDLINE

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2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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