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[PMID]: | 28632187 |
[Au] Autor: | Hsiao YH; Hsia SY; Chan YC; Hsieh JF |
[Ad] Endereço: | Ph.D. Program in Nutrition & Food Science, Fu Jen Catholic University, Taipei 24205, Taiwan. ariel.66@yahoo.com.tw. |
[Ti] Título: | Complex Coacervation of Soy Proteins, Isoflavones and Chitosan. |
[So] Source: | Molecules;22(6), 2017 Jun 20. | [Is] ISSN: | 1420-3049 |
[Cp] País de publicação: | Switzerland |
[La] Idioma: | eng |
[Ab] Resumo: | In this study, the chitosan-induced coacervation of soy protein-isoflavone complexes in soymilk was investigated. Most of the soymilk proteins, including ß-conglycinin (7S), glycinin (11S), and isoflavones, were found to coacervate into the soymilk pellet fraction (SPF) following the addition of 0.5% chitosan. The total protein in the soymilk supernatant fraction (SSF) decreased from 18.1 ± 0.3 mg/mL to 1.6 ± 0.1 mg/mL, and the pH values decreased slightly, from 6.6 ± 0.0 to 6.0 ± 0.0. The results of SDS-PAGE revealed that the 7S α', 7S α, 7S ß, 11S A3, and 11S acidic subunits, as well as the 11S basic proteins in the SSF, decreased to 0.7 ± 0.5%, 0.2 ± 0.1%, 0.1 ± 0.0%, 0.2 ± 0.2%, 0.2 ± 0.2% and 0.3 ± 0.2%, respectively. We also found that isoflavones in the SSF, including daidzein, glycitein, and genistein, decreased to 9.6 ± 2.3%, 5.7 ± 0.9% and 5.9 ± 1.5%, respectively. HPLC analysis indicated that isoflavones mixed with soy proteins formed soy protein-isoflavone complexes and were precipitated into the SPF by 0.5% chitosan. |
[Mh] Termos MeSH primário: |
Quitosana/química Proteínas de Soja/química
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[Mh] Termos MeSH secundário: |
Antígenos de Plantas/química Cromatografia Líquida de Alta Pressão Análise de Alimentos Globulinas/química Isoflavonas/química Avaliação Nutricional Proteínas de Armazenamento de Sementes/química Leite de Soja/química
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antigens, Plant); 0 (Globulins); 0 (Isoflavones); 0 (Seed Storage Proteins); 0 (Soybean Proteins); 0 (beta-conglycinin protein, Glycine max); 9012-76-4 (Chitosan); 92M5F28TVF (glycitein) |
[Em] Mês de entrada: | 1706 |
[Cu] Atualização por classe: | 170630 |
[Lr] Data última revisão:
| 170630 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170621 |
[St] Status: | MEDLINE |
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