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[PMID]: 29305261 [Au] Autor: Magni C; Sessa F; Capraro J; Duranti M; Maffioli E; Scarafoni A [Ad] Endereço: Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, Via G. Celoria, 2, 20133, Milan, Italy. [Ti] Título: Structural and functional insights into the basic globulin 7S of soybean seeds by using trypsin as a molecular probe. [So] Source: Biochem Biophys Res Commun;496(1):89-94, 2018 01 29. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The basic 7S globulin (Bg7S) is one of the major globulins of soybean seeds. Despite its dual subunit composition and oligomeric assembly, Bg7S has a compact 3D structure (PDB: 3AUP) which is stabilized by a network of inter- and intra-chain disulphide bridges. Bg7S shares several structural elements with a number of homologous proteins from other seeds, whose function is still uncertain. In this work, Bg7S native conformation was probed by using the proteolytic enzyme trypsin. In spite of the presence of many arginine and lysine residues, the protein resulted extremely recalcitrant to in vitro enzymatic cleavage. Indeed, only two scissile bonds located near the C- and N-termini of the large and small subunits, respectively, were cleaved. The partially cleaved products were stable even at prolonged incubation times. Although the generated small peptide fragments were not covalently bound to the remnant of the main chains, they were held in place, as assessed by denaturing and non-denaturing chromatographic approaches. Moreover, both the already observed pH-dependent association/dissociation behaviour of the protein and its insulin binding capacity were preserved both at neutral and acidic pH values. These results are in line with the growing view that the degradation of seed proteins, either storage and non-storage, may be a controlled process related to specific functionalities. [Mh] Termos MeSH primário: Globulinas/química Técnicas de Sonda Molecular Sementes/química Proteínas de Soja/química Feijão de Soja/química Tripsina/química [Mh] Termos MeSH secundário: Sítios de Ligação Modelos Químicos Modelos Moleculares Sondas Moleculares/química Ligação Proteica Conformação Proteica [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Globulins); 0 (Molecular Probes); 0 (Soybean Proteins); EC 3.4.21.4 (Trypsin) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180215 [Lr] Data última revisão: 180215 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180107 [St] Status: MEDLINE

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[PMID]: 29320758 [Au] Autor: Voutsadakis IA [Ad] Endereço: Division of Medical Oncology, Department of Internal Medicine, Sault Area Hospital, Sault Ste Marie, Ontario, Canada; Division of Clinical Sciences, Northern Ontario School of Medicine, Sudbury, Ontario, Canada. Electronic address: ivoutsadakis@nosm.ca. [Ti] Título: Polymerase epsilon mutations and concomitant ß2-microglobulin mutations in cancer. [So] Source: Gene;647:31-38, 2018 Mar 20. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Mutations in the exonuclease domain of polymerase epsilon (POLE), an enzyme of DNA synthesis, are involved in a newly described syndrome of colorectal polyposis and cancer, and have been associated with a high mutation burden with or without microsatellite instability (MSI) phenotype. The exonuclease domain of POLE executes a proofreading function that decreases the mutation rate during DNA replication by an estimated of one to two orders. The high mutation burden resulting from its loss of function could create a load of neo-antigens that would put the neoplastic cells in severe disadvantage of an immune attack if properly presented to the immune system. This paper investigates the mutagenic effect of different POLE mutations in various cancers, in published genomic studies and the effect that these POLE mutations have in selecting for mutations of the ß2 microglobulin (B2M) gene involved in antigen presentation. [Mh] Termos MeSH primário: DNA Polimerase II/genética Globulinas/genética Mutação/genética Neoplasias/genética Proteínas de Ligação a Poli-ADP-Ribose/genética [Mh] Termos MeSH secundário: Replicação do DNA/genética Exodesoxirribonucleases/genética Seres Humanos Instabilidade de Microssatélites Mutagênese/genética Taxa de Mutação Fenótipo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Globulins); 0 (Poly-ADP-Ribose Binding Proteins); EC 2.7.7.- (DNA Polymerase II); EC 2.7.7.7 (POLE protein, human); EC 3.1.- (Exodeoxyribonucleases) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180209 [Lr] Data última revisão: 180209 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180111 [St] Status: MEDLINE

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[PMID]: 29300750 [Au] Autor: Guo HW; Yuan TZ; Chen JX; Zheng Y [Ad] Endereço: Department of General Surgery, The Fourth Affiliated Hospital of Nan Chang University, Nanchang, Jiangxi, China. [Ti] Título: Prognostic value of pretreatment albumin/globulin ratio in digestive system cancers: A meta-analysis. [So] Source: PLoS One;13(1):e0189839, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The albumin/globulin ratio (AGR) has been widely reported to be a potential predictor of prognosis in digestive system cancers (DSCs), but convincing conclusions have not been made. Therefore, herein, we performed a meta-analysis of relevant studies regarding this topic to evaluate the prognostic value of AGR in patients with DSCs. Three databases, including PubMed, EMBase, and Web of science, were searched comprehensively for eligible studies through September 8, 2017. The outcomes of interest included overall survival (OS), disease-free survival (DFS), and cancer-specific survival (CSS). In our meta-analysis, pooled analysis of 13 studies with 9269 patients showed that a low AGR was significantly correlated with poor OS (HR = 1.94; 95% CI: 1.57-2.38; P <0.001). Five studies with 6538 participants involved DFS, and our pooled analysis of these studies also demonstrated that there was a significant association of a low AGR with worse DFS (HR = 1.49; 95% CI: 1.10 to 2.00; P < 0.001). In addition, only 2 studies referred to CSS, and we also detected a significant relationship between a low AGR and worse CSS from the results of our meta-analysis. In summary, a low pretreatment AGR was related to unfavorable survival in human digestive system cancers. A low pretreatment AGR may be a useful predictive prognostic biomarker in human digestive system cancers. [Mh] Termos MeSH primário: Albuminas/metabolismo Biomarcadores Tumorais/sangue Neoplasias do Sistema Digestório/patologia Globulinas/metabolismo [Mh] Termos MeSH secundário: Neoplasias do Sistema Digestório/sangue Seres Humanos Prognóstico Taxa de Sobrevida [Pt] Tipo de publicação: JOURNAL ARTICLE; META-ANALYSIS [Nm] Nome de substância: 0 (Albumins); 0 (Biomarkers, Tumor); 0 (Globulins) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180210 [Lr] Data última revisão: 180210 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180105 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0189839

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[PMID]: 29227096 [Au] Autor: Song B; Oehrle NW; Liu S; Krishnan HB [Ad] Endereço: Key Laboratory of Soybean Biology at the Chinese Ministry of Education, Northeast Agricultural University , Harbin 150030, China. [Ti] Título: Development and Characterization of a Soybean Experimental Line Lacking the α' Subunit of ß-Conglycinin and G1, G2, and G4 Glycinin. [So] Source: J Agric Food Chem;66(2):432-439, 2018 Jan 17. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A soybean experimental line (BSH-3) devoid of a subset of seed storage proteins was developed by crossing a mutant donor line "HS99B" with a Chinese cultivar "Dongnong47" (DN47). One-dimensional and high-resolution 2-D gel electrophoresis revealed the absence of G1 (A1 B , G2 (A B1 , and G4 (A A B glycinin and the α' subunit of ß-conglycinin in BSH-3 seeds. Despite the lack of these abundant seed proteins, BSH-3 seeds still accumulated 38% protein. BSH-3 seeds also accumulated high levels of free amino acids as compared with DN47 seeds, particularly arginine, and the amount of several essential amino acids were significantly elevated in BSH-3 seeds. Elevated accumulation of α and ß-subunit of ß-conglycinin, G5 glycinin, Kunitz trypsin inhibitor, and Bowman-Birk protease inhibitor indicates seed proteome rebalancing in BSH-3 seeds. Immunoblot analysis using sera from soybean allergic patients demonstrated the complete lack of a major allergen (α' subunit of ß-conglycinin) in BSH-3 seeds. However, elevated levels of other allergens were found in BSH-3 seeds due to proteome rebalancing. Transmission electron microscopy observation of mature seeds of BSH-3 revealed striking differences in the appearance of the protein storage vacuoles when compared with DN47. [Mh] Termos MeSH primário: Antígenos de Plantas/análise Globulinas/análise Proteínas de Armazenamento de Sementes/análise Proteínas de Soja/análise Feijão de Soja/química [Mh] Termos MeSH secundário: Cruzamento Eletroforese em Gel Bidimensional Sementes/química Sementes/genética Feijão de Soja/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, Plant); 0 (Globulins); 0 (Seed Storage Proteins); 0 (Soybean Proteins); 0 (beta-conglycinin protein, Glycine max); 9007-93-6 (glycinin) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180205 [Lr] Data última revisão: 180205 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171212 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b05011

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[PMID]: 28460738 [Au] Autor: Oki S; Toiyama Y; Okugawa Y; Shimura T; Okigami M; Yasuda H; Fujikawa H; Okita Y; Yoshiyama S; Hiro J; Kobayashi M; Ohi M; Araki T; Inoue Y; Mohri Y; Kusunoki M [Ad] Endereço: Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Graduate School of Medicine, Mie University, Mie 514-8507, Japan. [Ti] Título: Clinical burden of preoperative albumin-globulin ratio in esophageal cancer patients. [So] Source: Am J Surg;214(5):891-898, 2017 Nov. [Is] ISSN: 1879-1883 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Lower albumin-globulin ratio (AGR) is associated with increased mortality in several cancers. However, no studies have evaluated the relationship between the AGR and prognostic outcome in esophageal cancer (EC) patients. METHODS: To identify indicators of early recurrence and poor prognosis, we assessed the clinicopathological findings and preoperative laboratory data (carcinoembryonic antigen [CEA], squamous cell carcinoma antigen, total protein, and albumin) of 112 EC patients who underwent surgery. The AGR was calculated as albumin/(total protein-albumin). RESULTS: A lower AGR was significantly associated with tumor progression. The CEA level was an independent predictor for overall survival (OS) and disease-free survival (DFS). The AGR and CEA combination was identified as a feasible indicator of poor prognosis and early recurrence. Among EC patients without lymph node metastasis, those with lower AGR had poorer DFS and OS than those with higher AGR. CONCLUSION: AGR was identified as a significant predictor of OS and DFS in EC patients. Among EC patients without lymph node metastasis, AGR may help identify candidates who might benefit from more intensive adjuvant therapy. [Mh] Termos MeSH primário: Neoplasias Esofágicas/sangue Neoplasias Esofágicas/cirurgia Globulinas/análise Albumina Sérica/análise [Mh] Termos MeSH secundário: Adulto Idoso Idoso de 80 Anos ou mais Neoplasias Esofágicas/mortalidade Feminino Seres Humanos Masculino Meia-Idade Recidiva Local de Neoplasia/epidemiologia Período Pré-Operatório Prognóstico Taxa de Sobrevida [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Globulins); 0 (Serum Albumin) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171128 [Lr] Data última revisão: 171128 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 170503 [St] Status: MEDLINE

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[PMID]: 28867186 [Au] Autor: Ikaga R; Li D; Yamazaki T [Ad] Endereço: Department of Nutritional Science, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8636, Japan. [Ti] Título: Dietary ß-conglycinin prevents acute ethanol-induced fatty liver in mice. [So] Source: Biochem Biophys Res Commun;493(1):542-547, 2017 Nov 04. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Alcoholic fatty liver is the earliest stage of alcohol-induced liver disease leading to liver cirrhosis. ß-Conglycinin, one of the soy proteins, is known to prevent non-alcoholic fatty liver, hyperlipidemia and obesity. Therefore, we examined whether ß-conglycinin feeding has an effect on the prevention of acute ethanol-induced fatty liver in mice. Male C57BL/6J mice were fed with 20 energy% ß-conglycinin or casein for 4 weeks prior to ethanol administration and were then given ethanol or glucose, as a control, by gavage. Ethanol significantly increased liver triglyceride (TG) in mice fed casein due to the activation of peroxisome proliferator-activated receptor (PPAR) γ2, a nuclear transcription factor known for regulating lipid metabolism and de novo lipogenesis. The liver TG of ethanol-administered ß-conglycinin-fed mice was significantly lower than that in those fed casein, although ethanol increased the amount of liver TG in mice fed ß-conglycinin. The increased levels of PPARγ2 protein and its target gene CD36 in response to an ethanol were not observed in mice fed ß-conglycinin. Moreover, ß-conglycinin decreased the basal expression of de novo lipogenesis-related genes such as stearoyl-CoA desaturase-1, and therefore, the expressions of these genes were lower in the ethanol-administered ß-conglycinin-fed mice than in the casein-fed mice. In conclusion, ß-conglycinin supplementation appears to prevent the development of fatty liver in mice caused by ethanol consumption via the suppression of alcohol-induced activation of PPARγ2 and the downregulation of the basal expression of de novo lipogenesis. [Mh] Termos MeSH primário: Antígenos de Plantas/administração & dosagem Suplementos Nutricionais Globulinas/administração & dosagem Lipogênese/efeitos dos fármacos Hepatopatias Alcoólicas/metabolismo Hepatopatias Alcoólicas/prevenção & controle PPAR gama/metabolismo Proteínas de Armazenamento de Sementes/administração & dosagem Proteínas de Soja/administração & dosagem [Mh] Termos MeSH secundário: Animais Relação Dose-Resposta a Droga Etanol/envenenamento Hepatopatias Alcoólicas/etiologia Masculino Camundongos Camundongos Endogâmicos C57BL Resultado do Tratamento [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, Plant); 0 (Globulins); 0 (PPAR gamma); 0 (Seed Storage Proteins); 0 (Soybean Proteins); 0 (beta-conglycinin protein, Glycine max); 3K9958V90M (Ethanol) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171023 [Lr] Data última revisão: 171023 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170905 [St] Status: MEDLINE

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[PMID]: 28635255 [Au] Autor: Wu W; Zhang M; Ren Y; Cai X; Yin Z; Zhang X; Min T; Wu H [Ad] Endereço: College of Food Sciences and Engineering, South China University of Technology , Guangzhou, Guangdong 510640, People's Republic of China. [Ti] Título: Characterization and Immunomodulatory Activity of a Novel Peptide, ECFSTA, from Wheat Germ Globulin. [So] Source: J Agric Food Chem;65(27):5561-5569, 2017 Jul 12. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A novel peptide was extracted from wheat germ globulin and purified using ion-exchange chromatography, gel filtration chromatography, and semi-preparative reversed-phase high-performance liquid chromatography (RP-HPLC). The sequence of the peptide was found to be Glu-Cys-Phe-Ser-Thr-Ala (ECFSTA). Its immunomodulatory effects were evaluated, and the results showed that ECFSTA could enhance phagocytosis of RAW 264.7 cells and significantly increase their secretion of nitric oxide (NO), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and reactive oxygen species (ROS). ECFSTA activated macrophages mainly through the pattern recognition receptors (PRRs) of toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4), and the production of ROS simultaneously stimulated macrophages to produce TNF-α. Thus, ECFSTA could be used as an immunomodulator and might be a promising component of functional foods. [Mh] Termos MeSH primário: Globulinas/farmacologia Fatores Imunológicos/farmacologia Peptídeos/farmacologia Triticum/química [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Globulinas/química Fatores Imunológicos/química Macrófagos/efeitos dos fármacos Macrófagos/imunologia Camundongos Dados de Sequência Molecular Mapeamento de Peptídeos Peptídeos/química Células RAW 264.7 [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Globulins); 0 (Immunologic Factors); 0 (Peptides) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170720 [Lr] Data última revisão: 170720 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170622 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b01360

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[PMID]: 28632187 [Au] Autor: Hsiao YH; Hsia SY; Chan YC; Hsieh JF [Ad] Endereço: Ph.D. Program in Nutrition & Food Science, Fu Jen Catholic University, Taipei 24205, Taiwan. ariel.66@yahoo.com.tw. [Ti] Título: Complex Coacervation of Soy Proteins, Isoflavones and Chitosan. [So] Source: Molecules;22(6), 2017 Jun 20. [Is] ISSN: 1420-3049 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: In this study, the chitosan-induced coacervation of soy protein-isoflavone complexes in soymilk was investigated. Most of the soymilk proteins, including ß-conglycinin (7S), glycinin (11S), and isoflavones, were found to coacervate into the soymilk pellet fraction (SPF) following the addition of 0.5% chitosan. The total protein in the soymilk supernatant fraction (SSF) decreased from 18.1 ± 0.3 mg/mL to 1.6 ± 0.1 mg/mL, and the pH values decreased slightly, from 6.6 ± 0.0 to 6.0 ± 0.0. The results of SDS-PAGE revealed that the 7S α', 7S α, 7S ß, 11S A3, and 11S acidic subunits, as well as the 11S basic proteins in the SSF, decreased to 0.7 ± 0.5%, 0.2 ± 0.1%, 0.1 ± 0.0%, 0.2 ± 0.2%, 0.2 ± 0.2% and 0.3 ± 0.2%, respectively. We also found that isoflavones in the SSF, including daidzein, glycitein, and genistein, decreased to 9.6 ± 2.3%, 5.7 ± 0.9% and 5.9 ± 1.5%, respectively. HPLC analysis indicated that isoflavones mixed with soy proteins formed soy protein-isoflavone complexes and were precipitated into the SPF by 0.5% chitosan. [Mh] Termos MeSH primário: Quitosana/química Proteínas de Soja/química [Mh] Termos MeSH secundário: Antígenos de Plantas/química Cromatografia Líquida de Alta Pressão Análise de Alimentos Globulinas/química Isoflavonas/química Avaliação Nutricional Proteínas de Armazenamento de Sementes/química Leite de Soja/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, Plant); 0 (Globulins); 0 (Isoflavones); 0 (Seed Storage Proteins); 0 (Soybean Proteins); 0 (beta-conglycinin protein, Glycine max); 9012-76-4 (Chitosan); 92M5F28TVF (glycitein) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170630 [Lr] Data última revisão: 170630 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170621 [St] Status: MEDLINE

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[PMID]: 28590128 [Au] Autor: Zhao GP; Li YQ; Sun GJ; Mo HZ [Ad] Endereço: School of Food Science & Engineering, Qilu University of Technology , No. 3501 University Road of Changqing District, 250353, Jinan, Shandong Province China. [Ti] Título: Antibacterial Actions of Glycinin Basic Peptide against Escherichia coli. [So] Source: J Agric Food Chem;65(25):5173-5180, 2017 Jun 28. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Glycinin basic peptide (GBP) is an antibacterial ingredient that occurs naturally in the basic parts of soybean glycinin. The antibacterial actions of GBP against Escherichia coli ATCC 8739 were investigated in this study. The minimum inhibitory concentration of GBP against E. coli was 200 µg/mL. The exposure of E. coli cells to GBP induced significant cell damage and inactivated intracellular esterases (stressed and dead cells, 70.9% ± 0.04 for 200 µg/mL of GBP and 91.9% ± 0.06 for 400 µg/mL of GBP), as determined through dual staining in flow cytometry. GBP resulted in the exposure of phosphatidylserine in E. coli cells. The analyses of flow cytometry-manifested GBP treatment led to the shrinkage of the cell surface and the complication of cell granularity. The observations in transmission electron microscopy demonstrated that 400 µg/mL of GBP severely disrupted the membrane integrity, resulting in ruptures or pores in the membrane, outflows of intracellular contents, or aggregation of the cytoplasm. Release of alkaline phosphatase, lipopolysaccharide, and reducing sugar further verified that the membrane damage was due to GBP. In addition, GBP treatment changed the helicity and base staking of DNA, as determined by circular dichroism spectroscopy. These results showed that GBP had strong antibacterial activity against E. coli via membrane damage and DNA perturbation. Additionally, GBP exhibited no cytotoxicity on the viability of human embryonic kidney cells. Thus, GBP may be a promising candidate as a natural antibacterial agent. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Escherichia coli/efeitos dos fármacos Globulinas/farmacologia Peptídeos/farmacologia Proteínas de Soja/farmacologia [Mh] Termos MeSH secundário: Membrana Celular/efeitos dos fármacos Escherichia coli/citologia Escherichia coli/crescimento & desenvolvimento Infecções por Escherichia coli/microbiologia Seres Humanos Testes de Sensibilidade Microbiana [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Globulins); 0 (Peptides); 0 (Soybean Proteins); 9007-93-6 (glycinin) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170707 [Lr] Data última revisão: 170707 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170608 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b02295

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[PMID]: 28526454 [Au] Autor: Liu WG; Wang YP; Zhang ZJ; Wang M; Lv QX; Liu HW; Meng LC; Lu M [Ad] Endereço: Jilin Academy of Agricultural Sciences, Changchun, 130124, People's Republic of China. [Ti] Título: Generation and characterization of caprine chymosin in corn seed. [So] Source: Protein Expr Purif;135:78-82, 2017 Jul. [Is] ISSN: 1096-0279 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Chymosin is widely used in the dairy industry, and much is produced through recombinant DNA in organisms such as bacteria and tobacco. In this study, we used a new transgenic method to express caprine chymosin in corn seeds with lower cost and better storage capability. The recombinant chymosin protein was successfully expressed at an average level of 0.37 mg/g dry weight, which is 0.27% of the total soluble protein in the corn seed. Prochymosin can be activated to produce a chymosin protein with the ability to induce clotting in milk, similar to the commercial protein. The activity of the purified recombinant chymosin was as high as 178.5 U/mg. These results indicate that we have successfully established a technology for generating corn seed-derived caprine chymosin for potential use in the dairy industry. [Mh] Termos MeSH primário: Quimosina/biossíntese Vetores Genéticos/química Plantas Geneticamente Modificadas Sementes/genética Zea mays/genética [Mh] Termos MeSH secundário: Agrobacterium tumefaciens/genética Agrobacterium tumefaciens/metabolismo Animais Quimosina/genética Quimosina/isolamento & purificação Quimosina/farmacologia Clonagem Molecular Ensaios Enzimáticos Floculação/efeitos dos fármacos Tecnologia de Alimentos Expressão Gênica Vetores Genéticos/metabolismo Globulinas/genética Globulinas/metabolismo Cabras Cinética Leite/química Leite/efeitos dos fármacos Proteínas de Plantas/genética Proteínas de Plantas/metabolismo Regiões Promotoras Genéticas Proteínas Recombinantes/biossíntese Proteínas Recombinantes/genética Proteínas Recombinantes/isolamento & purificação Proteínas Recombinantes/farmacologia Sementes/enzimologia Transformação Genética Zea mays/enzimologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Globulins); 0 (Plant Proteins); 0 (Recombinant Proteins); EC 3.4.23.4 (Chymosin) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170731 [Lr] Data última revisão: 170731 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170521 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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