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Pesquisa : D12.776.377.715.390 [Categoria DeCS]
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[PMID]:29381744
[Au] Autor:Biniaminov N; Bandt S; Roth A; Haertel S; Neumann R; Bub A
[Ad] Endereço:Institute of Sports and Sports Science, Karlsruhe Institute of Technology, Karlsruhe, Germany.
[Ti] Título:Irisin, physical activity and fitness status in healthy humans: No association under resting conditions in a cross-sectional study.
[So] Source:PLoS One;13(1):e0189254, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Regular physical activity and physical fitness are closely related to a positive health status in humans. In this context, the muscle becomes more important due to its function as an endocrine organ. Muscle tissue secretes "myokines" in response to physical activity and it is speculated that these myokines are involved in physical activity induced positive health effects. Recently, the newly discovered myokine Irisin thought to be secreted by the muscle in response to physical activity and might be related to the health inducing effect by inducing browning of white adipose tissue. Speculating that myokines at least partly mediate exercise related health effects one would assume that regular physical activity and physical fitness are associated with resting Irisin concentrations in healthy humans. To investigate the association between resting Irisin concentration and either short-term physical activity, habitual physical activity, or physical fitness, data of 300 healthy participants from the cross-sectional KarMeN-study were analyzed. By applying different activity measurements we determined short-term and habitual physical activity, as well as physical fitness. Fasting serum samples were collected to determine resting Irisin concentrations by Enzyme-linked Immunosorbent Assay. Multivariate linear regression analysis served to investigate associations of the individual physical activity parameters with Irisin concentrations. Therefore, lean body mass and total fat mass (both determined by dual-energy X-ray absorptiometry) as well as age and parameters of glucose metabolism were included as confounders in multivariate linear regression analysis. Results showed that Irisin serum concentrations were not related to measures of physical activity and physical fitness in healthy humans under resting conditions, irrespective of the applied methods. Therefore we assume that if physical activity related effects are partly induced by myokines, permanently increased Irisin serum concentration may not be necessary to induce health-related exercise effects.
[Mh] Termos MeSH primário: Fibronectinas/metabolismo
Aptidão Física
Descanso
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Calorimetria
Estudos Transversais
Feminino
Seres Humanos
Masculino
Meia-Idade
Músculo Esquelético/secreção
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (FNDC5 protein, human); 0 (Fibronectins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189254


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[PMID]:29310440
[Au] Autor:Kodama H; Kumai Y; Nishimoto K; Toya Y; Miyamaru S; Furushima S; Yumoto E
[Ad] Endereço:1 Department of Otolaryngology Head and Neck Surgery, Kumamoto University Graduate School of Medicine, Kumamoto, Japan.
[Ti] Título:The Ferret as a Surgical Model for Vocal Fold Scar Creation and Treatment.
[So] Source:Ann Otol Rhinol Laryngol;127(3):146-154, 2018 Mar.
[Is] ISSN:1943-572X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To develop a vocal fold (VF) scarring procedure in the ferret, characterize the scars histologically, and test the injectability of the lamina propria (LP). Secondarily, to compare laryngeal anatomy of the ferret with rat and rabbit. MATERIALS AND METHODS: The larynges of 18 male ferrets were prepared by unilateral scarring, and normal larynges from 6 female Wistar rats and 5 male albino rabbits were used for comparative purposes. For scarring, the right VF were electrocauterized, ablating the entire LP. Prior to harvesting the larynges at 4 and 16 weeks, each ferret was re-anesthetized, and in 3 animals, India ink was injected into the LPs of both normal and scarred VFs. RESULTS: Laryngoscopic methods and instrumentation for precise visualization, scarring, and injection were developed. The scarred VFs had reduced hyaluronic acid and increased collagen type I, III, and fibronectin compared with normal VFs. The 2 timepoints (4 and 16 weeks) differed significantly only in collagen type III level (levels were higher at 4 weeks). Injected ink migrated from scarred LP to muscle layer just beneath the scarred tissue 3 hours after injection. CONCLUSION: The ferret is a promising species for creation and experimental treatment of vocal fold scar.
[Mh] Termos MeSH primário: Cicatriz
Eletrocoagulação/métodos
Laringoscopia
Membrana Mucosa
Prega Vocal/cirurgia
[Mh] Termos MeSH secundário: Animais
Cicatriz/etiologia
Cicatriz/metabolismo
Cicatriz/patologia
Colágeno Tipo I/análise
Colágeno Tipo III/análise
Feminino
Furões
Fibronectinas/análise
Laringoscopia/instrumentação
Laringoscopia/métodos
Modelos Anatômicos
Membrana Mucosa/patologia
Membrana Mucosa/cirurgia
Coelhos
Ratos
Prega Vocal/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type I); 0 (Collagen Type III); 0 (Fibronectins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1177/0003489417750165


  3 / 22529 MEDLINE  
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[PMID]:29297640
[Au] Autor:Kolesnikova LI; Madaeva IM; Semenova NV; Osipova EV; Darenskaya MA
[Ti] Título:Serum Myokines Levels in Patients with Endogenous Cushing Syndrome and Acromegaly: Cross-Sectional Case−Control Study.
[So] Source:Vestn Ross Akad Med Nauk;71(3):240-7, 2016.
[Is] ISSN:0869-6047
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Background: Myokines are produced and released by muscle cells in response to muscular contractions. Endogenous Cushing syndrome (CS) and acromegaly cause significant changes in muscle tissue leading to atrophy or hypertrophy. However, there is no data whether these endocrine abnormalities influence myokine secretion. Aims: To evaluate serum levels of myostatin, interleukin-6 (IL6) and irisin in patients with CS and acromegaly. Materials and Methods: Fasting serum samples were taken and stored in aliquot at ≤-20°C from consecutive subjects with clinically evident and biochemically confirmed active CS, acromegaly and healthy volunteers matched by age, sex and body mass index (BMI). Commercially available kits were used to assay serum myokine levels. Grip strength was measured by a dynamometer. Insulin-like growth factor-1 (IGF1) was measured by immunochemiluminescence assay (Liaison), twenty-four hours urine free cortisol (24hUFC) ­ by immunochemiluminescence assay (Vitros ECi), salivary free cortisol ­ by electrochemiluminescence assay (Cobas). One-way ANOVA was utilized to assess the difference between groups. Results: We enrolled 88 subjects: 30 patients suffered from CS (group 1), 28 ­ acromegaly (2) and 30 matched healthy controls (3) with no difference among the groups in sex, age and BMI (p=0.492, 0.062 and 0.174 respectively). Mean 24hUFC in subjects with CS and mean IGF1 in subjects with acromegaly were significantly higher as compared to other groups (p<0.001). Right-hand grip strength was lower in patients with CS as compared to both patients with acromegaly and healthy subjects (p=0.04). However, among these young adults we did not find statistically significant differences in measured myokines levels: irisin ­ p=0.15; IL6 ­ p=0.34; myostatin ­ p=0.50. There was a significant correlation between myostatin and irisin in the whole group of people and in every separately analyzed subset of patients (p<0.001), but no correlation was found between any measured myokines and 24hUFC or IGF1. Conclusions: Hypercortisolism or supraphysiological IGF1 levels do not significantly influence serum levels of myostatin, IL6 and irisin in young adults.
[Mh] Termos MeSH primário: Acromegalia
Fibronectinas/sangue
Fator de Crescimento Insulin-Like I
Interleucina-6/sangue
Músculo Esquelético
Miostatina/sangue
[Mh] Termos MeSH secundário: Acromegalia/etiologia
Acromegalia/metabolismo
Acromegalia/fisiopatologia
Adulto
Síndrome de Cushing/complicações
Feminino
Seres Humanos
Fator de Crescimento Insulin-Like I/análise
Fator de Crescimento Insulin-Like I/metabolismo
Masculino
Contração Muscular/fisiologia
Músculo Esquelético/metabolismo
Músculo Esquelético/fisiopatologia
Estatística como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FNDC5 protein, human); 0 (Fibronectins); 0 (Interleukin-6); 0 (Myostatin); 67763-96-6 (Insulin-Like Growth Factor I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.15690/vramn659


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[PMID]:29337054
[Au] Autor:Han SJ; Jung JK; Im SS; Lee SR; Jang BC; Park KM; Kim JI
[Ad] Endereço:Department of Anatomy and BK21 Plus, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea.
[Ti] Título:Deficiency of primary cilia in kidney epithelial cells induces epithelial to mesenchymal transition.
[So] Source:Biochem Biophys Res Commun;496(2):450-454, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Primary cilium is a microtubule-based non-motile organelle that plays critical roles in kidney pathophysiology. Our previous studies revealed that the lengths of primary cilia decreased upon renal ischemia/reperfusion injury and oxidative stress, and restored with recovery. Here, we tested the hypothesis that lack of primary cilium causes epithelial to mesenchymal transition (EMT) of kidney tubule cells. We investigated the alteration of length of primary cilia in TGF-ß-induced EMT via visualization of primary cilia by fluorescence staining against acetylated α-tubulin. EMT was determined by measuring mesenchymal protein expression using quantitative PCR and indirect fluorescence staining. As a result, TGF-ß treatment decreased ciliary length along with EMT. To test whether defect of primary cilia trigger onset of EMT, cilia formation was disturbed by knock down of ciliary protein using siRNA along with/without TGF-ß treatment. Knock down of Arl13b and Ift20 reduced cilia elongation and increased expression of EMT markers such as fibronectin, α-SMA, and collagen III. TGF-ß-induced EMT was greater as well in Arl13b and Ift20-knock down cells compared to control cells. Taken together, deficiency of primary cilia trigger EMT and exacerbates it under pro-fibrotic signals.
[Mh] Termos MeSH primário: Cílios/efeitos dos fármacos
Transição Epitelial-Mesenquimal/efeitos dos fármacos
Fator de Crescimento Transformador beta/farmacologia
Tubulina (Proteína)/genética
[Mh] Termos MeSH secundário: Fatores de Ribosilação do ADP/antagonistas & inibidores
Fatores de Ribosilação do ADP/genética
Fatores de Ribosilação do ADP/metabolismo
Actinas/genética
Actinas/metabolismo
Animais
Proteínas de Transporte/antagonistas & inibidores
Proteínas de Transporte/genética
Proteínas de Transporte/metabolismo
Tamanho Celular
Cílios/metabolismo
Cílios/ultraestrutura
Colágeno Tipo III/genética
Colágeno Tipo III/metabolismo
Cães
Transição Epitelial-Mesenquimal/genética
Fibronectinas/genética
Fibronectinas/metabolismo
Regulação da Expressão Gênica
Células Madin Darby de Rim Canino
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Transdução de Sinais
Tubulina (Proteína)/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (Carrier Proteins); 0 (Collagen Type III); 0 (Fibronectins); 0 (RNA, Small Interfering); 0 (Transforming Growth Factor beta); 0 (Tubulin); EC 3.6.5.2 (ADP-Ribosylation Factors)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


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[PMID]:28459181
[Au] Autor:Yang SL; Zhu LY; Han R; Sun LL; Dou JT
[Ad] Endereço:1 Department of Endocrinology, Chinese PLA General Hospital (301 Hospital), Beijing, China.
[Ti] Título:Effect of Negative Pressure Wound Therapy on Cellular Fibronectin and Transforming Growth Factor-ß1 Expression in Diabetic Foot Wounds.
[So] Source:Foot Ankle Int;38(8):893-900, 2017 Aug.
[Is] ISSN:1944-7876
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Chronic diabetic foot wounds are a leading cause of amputation, morbidity, and hospitalization for patients with diabetes. Negative-pressure wound therapy (NPWT) can putatively facilitate wound healing, but the underlying mechanisms remain unclear. Cellular fibronectin (cFN) and transforming growth factor-ß1 (TGF-ß1) play an important role in wound healing. This prospective randomized controlled trial evaluated the effects of NPWT on the production of cFN and the expression of TGF-ß1 in diabetic foot wounds of patients. METHODS: From January 2012 to January 2015, 40 patients with diabetic foot wounds were randomly and equally apportioned to receive either NPWT or advanced moist wound therapy (control) for 7 days. Granulation tissue was harvested before and after treatment. Immunohistochemistry and Western blot were performed to evaluate protein levels of cFN and TGF-ß1, and real-time polymerase chain reaction (PCR) to measure corresponding mRNA expressions. RESULTS: NPWT facilitated the expression of cFN and TGF-ß1 in diabetic foot wounds. Immunohistochemical analysis revealed higher levels of cFN and TGF-ß1 in the NPWT group than in the control group. Western blot and real-time PCR analysis further showed that protein and mRNA levels of cFN or TGF-ß1 were higher in the NPWT group than that in the control group ( P < .01, both). CONCLUSION: Our results showed that NPWT facilitated the production of cFN and the expression of TGF-ß1 in granulation tissue in diabetic foot ulcers. LEVEL OF EVIDENCE: Level I, randomized controlled study.
[Mh] Termos MeSH primário: Pé Diabético/metabolismo
Pé Diabético/terapia
Fibronectinas/metabolismo
Tratamento de Ferimentos com Pressão Negativa
Fator de Crescimento Transformador beta1/metabolismo
Fator de Crescimento Transformador beta1/fisiologia
Fatores de Crescimento Transformadores/metabolismo
[Mh] Termos MeSH secundário: Amputação
Fibronectinas/fisiologia
Seres Humanos
Tratamento de Ferimentos com Pressão Negativa/métodos
Estudos Prospectivos
Fatores de Crescimento Transformadores/fisiologia
Cicatrização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fibronectins); 0 (Transforming Growth Factor beta1); 0 (migration stimulating factor, human); 76057-06-2 (Transforming Growth Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1177/1071100717704940


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[PMID]:29202303
[Au] Autor:Sankiewicz A; Romanowicz L; Pyc M; Hermanowicz A; Gorodkiewicz E
[Ad] Endereço:Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland. Electronic address: ania@uwb.edu.pl.
[Ti] Título:SPR imaging biosensor for the quantitation of fibronectin concentration in blood samples.
[So] Source:J Pharm Biomed Anal;150:1-8, 2018 Feb 20.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was presentation of a new biosensor capable of determination of fibronectin. This biosensor was based on the specific interaction of anti-fibronectin antibody produced in rabbit with fibronectin. The surface plasmon resonance imaging (SPRI) technique was used as a detecting method. Optimization and characterization properties of the biosensor were studied. The determination of fibronectin concentration in natural samples was done. The results were compared with a reference method (Enzyme-Linked Immunosorbent Assay-ELISA). The analytically useful dynamic response range of biosensor is between 5 and 400ngmL . The detection limit is 1.5ngmL and limit quantification is 5ngmL . The proposed SPRI biosensor showed good selectivity for potential interferences. It was applied to determine fibronectin concentrations in plasma of healthy donors and of patients after thermal injury. Good correlations between results obtained using the SPRI biosensor and ELISA test (correlation coefficients for healthy donors 0.996, for patients 0.984) were obtained. The average fibronectin concentration of healthy donors was 140.5±24.6µgmL and the average fibronectin concentration of patients was 601.5±72.1µgmL , which was in agreement with results obtained by other investigators. The obtained results indicate that the developed biosensor may be a candidate for monitoring fibronectin concentration in blood samples.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Queimaduras/sangue
Fibronectinas/sangue
Ressonância de Plasmônio de Superfície/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Estudos de Casos e Controles
Criança
Pré-Escolar
Ensaio de Imunoadsorção Enzimática
Fibronectinas/análise
Seres Humanos
Lactente
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fibronectins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


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[PMID]:28448446
[Au] Autor:Wu Y; Wang L; Deng D; Zhang Q; Liu W
[Ad] Endereço:Department of Nephrology, Affiliated Beijing Friendship Hospital, Faculty of Kidney Diseases, Capital Medical University, No. 95 Yong An Road, Xi Cheng District, Beijing 100050, China. wyryyyy@163.com.
[Ti] Título:Renalase Protects against Renal Fibrosis by Inhibiting the Activation of the ERK Signaling Pathways.
[So] Source:Int J Mol Sci;18(5), 2017 Apr 27.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Renal interstitial fibrosis is a common pathway for the progression of chronic kidney disease (CKD) to end-stage renal disease. Renalase, acting as a signaling molecule, has been reported to have cardiovascular and renal protective effects. However, its role in renal fibrosis remains unknown. In this study, we evaluated the therapeutic efficacy of renalase in rats with complete unilateral ureteral obstruction (UUO) and examined the inhibitory effects of renalase on transforming growth factor-ß1 (TGF-ß1)-induced epithelial-mesenchymal transition (EMT) in human proximal renal tubular epithelial (HK-2) cells. We found that in the UUO model, the expression of renalase was markedly downregulated and adenoviral-mediated expression of renalase significantly attenuated renal interstitial fibrosis, as evidenced by the maintenance of E-cadherin expression and suppressed expression of α-smooth muscle actin (α-SMA), fibronectin and collagen-I. In vitro, renalase inhibited TGF-ß1-mediated upregulation of α-SMA and downregulation of E-cadherin. Increased levels of Phospho-extracellular regulated protein kinases (p-ERK1/2) in TGF-ß1-stimulated cells were reversed by renalase cotreatment. When ERK1 was overexpressed, the inhibition of TGF-ß1-induced EMT and fibrosis mediated by renalase was attenuated. Our study provides the first evidence that renalase can ameliorate renal interstitial fibrosis by suppression of tubular EMT through inhibition of the ERK pathway. These results suggest that renalase has potential renoprotective effects in renal interstitial fibrosis and may be an effective agent for slowing CKD progression.
[Mh] Termos MeSH primário: Sistema de Sinalização das MAP Quinases/fisiologia
Monoaminoxidase/metabolismo
[Mh] Termos MeSH secundário: Adenoviridae/genética
Animais
Linhagem Celular
Modelos Animais de Doenças
Regulação para Baixo
Transição Epitelial-Mesenquimal/efeitos dos fármacos
Fibronectinas/metabolismo
Fibrose/fisiopatologia
Vetores Genéticos/genética
Vetores Genéticos/metabolismo
Seres Humanos
Nefropatias/etiologia
Nefropatias/metabolismo
Nefropatias/patologia
Masculino
Proteína Quinase 1 Ativada por Mitógeno/genética
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Proteína Quinase 3 Ativada por Mitógeno/genética
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Monoaminoxidase/sangue
Monoaminoxidase/genética
Ratos
Ratos Sprague-Dawley
Fator de Crescimento Transformador beta1/farmacologia
Regulação para Cima
Obstrução Ureteral/complicações
Obstrução Ureteral/metabolismo
Obstrução Ureteral/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fibronectins); 0 (Transforming Growth Factor beta1); EC 1.4.3.4 (Monoamine Oxidase); EC 1.4.3.4. (renalase); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


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[PMID]:29244922
[Au] Autor:Kuznik BI; Davydov SO; Stepanov AV; Morar NV
[Ti] Título:The Effect of Kinesitherapy Exercises on the Level of Irisin among Females with Cardio-vascular diseases depending on the body mass and hormonal status.
[So] Source:Patol Fiziol Eksp Ter;60(4):47-51, 2016 Oct-Dec.
[Is] ISSN:0031-2991
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The observation was conducted on 41 female subjects age 32 to 69 with compensated cardio-vascular diseases. 23 of the subjects had an increased body mass index (BMI). It was established that the older the females the less of the irisin muscle hormone is found in the blood. In the subjects with a higher BMI the level of irisin in the blood is also higher. Direct correlations were found between the level of irisin and the level of female sex hormones - estrogen and progesterone. Under the effect of kinesitherapy exercises the level of irisin in females with normal BMI increases; whereas in the females with a higher BMI it generally stays the same or is decreased. The characteristics of irisin's response to the kinesitherapy exercises depends on its original level, the intensity of physical exercise and the subject's physique.
[Mh] Termos MeSH primário: Índice de Massa Corporal
Doenças Cardiovasculares
Estrogênios/sangue
Terapia por Exercício
Fibronectinas/sangue
Progesterona/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Doenças Cardiovasculares/sangue
Doenças Cardiovasculares/terapia
Feminino
Seres Humanos
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogens); 0 (FNDC5 protein, human); 0 (Fibronectins); 4G7DS2Q64Y (Progesterone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE


  9 / 22529 MEDLINE  
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[PMID]:29196262
[Au] Autor:Nishida S; Yoshizaki H; Yasui Y; Kuwahara T; Kiyokawa E; Kohno M
[Ad] Endereço:Department of Pediatric Surgery, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa 920-0293, Japan.
[Ti] Título:Collagen VI suppresses fibronectin-induced enteric neural crest cell migration by downregulation of focal adhesion proteins.
[So] Source:Biochem Biophys Res Commun;495(1):1461-1467, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The enteric nervous system (ENS) is a network of neurons and glia that are derived from enteric neural crest cells (ENCCs) and essential for regulating peristaltic activity of the colon. ENCCs migrate along the gastrointestinal tract to form the ENS, and disruption of ENCC motility leads to ENS disorders, such as Hirschsprung's disease. Previous ENCC-transplant experiments show that ENCCs can invade into isolated mouse intestines by age E13.5, but not after E15.5. We hypothesized that altered age-specific micro-environments in the intestine are responsible for ENCC invasion/migration. Here, we compared gene expression in the intestine between at E11.5 and E15.5 and identified 1355 differentially expressed transcripts. Among these, we found that genes encoding extracellular matrix (ECM) proteins were enriched. Notably, collagen VI (ColVI) family members were upregulated in the E15.5 mouse intestine at the mRNA and protein levels, whereas fibronectin (FN) was downregulated; however, both proteins showed colocalization at E15.5. To understand the mechanisms of ColVI and FN in ENCC migration, we examined neurosphere or individual ENCC-adherence capabilities toward the ECM. ColVI suppressed FN-induced ENCC spreading/migration, whereas ColVI induced morphologically narrow ENCC spreading and weak stress-fiber formation as compared with those with FN. Additionally, in ENCCs cultured on plates containing ColVI, the expression and phosphorylation of p130 , a members of focal adhesion complexes, was reduced. These data indicated an inhibitory role of ColVI in ENCC migration and suggested that ColVI suppression in the intestine might represent a novel therapeutic strategy for aganglionic colonic diseases.
[Mh] Termos MeSH primário: Movimento Celular/fisiologia
Colágeno Tipo VI/metabolismo
Sistema Nervoso Entérico/metabolismo
Proteínas da Matriz Extracelular/metabolismo
Fibronectinas/metabolismo
Adesões Focais/metabolismo
Crista Neural/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Regulação para Baixo/fisiologia
Sistema Nervoso Entérico/citologia
Camundongos
Camundongos Endogâmicos C57BL
Crista Neural/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Collagen Type VI); 0 (Extracellular Matrix Proteins); 0 (Fibronectins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE


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[PMID]:29174387
[Au] Autor:Zhao C; Xu Z; Wang Z; Suo C; Tao J; Han Z; Gu M; Tan R
[Ad] Endereço:Department of Urology, Nanjing Medical University First Affiliated Hospital, Nanjing 210029, China.
[Ti] Título:Role of tumor necrosis factor-α in epithelial-to-mesenchymal transition in transplanted kidney cells in recipients with chronic allograft dysfunction.
[So] Source:Gene;642:483-490, 2018 Feb 05.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Chronic allograft dysfunction (CAD) is characterized by allograft kidney interstitial fibrosis, the underlying mechanism of which is unclear. Our aim was to elucidate the role and mechanism of TNF-α-induced epithelial-to-mesenchymal transition (EMT) in transplant kidney tubular interstitial fibrosis. METHODS: Human kidney tissues from normal volunteers and CAD patients were assessed using periodic acid-Schiff, Masson trichrome and immunohistochemical staining. mRNA and protein expression of E-cadherin, α-smooth muscle actin (SMA) and fibronectin(FN) in renal proximal tubule epithelial (HK-2) cells after treatment with TNF-α under different conditions were assessed using western blot and qRT-PCR analysis. Cell motility and migration were assessed using wound healing and transwell assays. Expression of Smurf2 and TNF-α-signaling pathway-related proteins in HK-2 cells treated with TNF-α was detected by western blotting. E-cadherin and α-SMA expression was also assessed in Smurf2 plasmid-transfected or Smurf2 siRNA-treated HK-2 cells. RESULTS: The expression of TNF-α, Smurf2, α-SMA, and fibronectin was significantly upregulated, while the expression of E-cad was downregulated in the CAD group compared with the normal group. The in vitro results showed that TNF-α remarkably upregulated the expression of Smurf2, α-SMA and fibronectin and downregulated the expression of E-cadherin in HK-2 cells and enhanced motility and migration in HK-2 cells. Overexpression of Smurf2 could promote the expression of α-SMA and inhibit the expression of E-cad, whereas knockdown of Smurf2 expression reversed TNF-α-induced upregulation of α-SMA and prohibited the reduction of E-cad expression. Furthermore, TNF-α-induced Smurf2 expression promoted EMT through the Akt signaling pathway. CONCLUSIONS: TNF-α induced EMT via the TNF-α/Akt/Smurf2 signaling pathways, and it may play a role in aggravating allograft kidney interstitial fibrosis in CAD patients.
[Mh] Termos MeSH primário: Transição Epitelial-Mesenquimal
Transplante de Rim/efeitos adversos
Disfunção Primária do Enxerto/patologia
Fator de Necrose Tumoral alfa/metabolismo
[Mh] Termos MeSH secundário: Actinas/genética
Actinas/metabolismo
Adulto
Caderinas/genética
Caderinas/metabolismo
Linhagem Celular
Movimento Celular
Feminino
Fibronectinas/genética
Fibronectinas/metabolismo
Regulação da Expressão Gênica
Seres Humanos
Masculino
Disfunção Primária do Enxerto/genética
Disfunção Primária do Enxerto/metabolismo
Transdução de Sinais
Fator de Necrose Tumoral alfa/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ACTA2 protein, human); 0 (Actins); 0 (CDH1 protein, human); 0 (Cadherins); 0 (Fibronectins); 0 (TNF protein, human); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE



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