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[PMID]:28095357
[Au] Autor:Luo C; Chen S; Xu N; Sai WB; Zhao W; Li YC; Hu XJ; Tian H; Gao XD; Yao WB
[Ad] Endereço:Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009 China.
[Ti] Título:Establishment of a fluorescence-based method to evaluate endocytosis of desialylated glycoproteins in vitro.
[So] Source:Biomed Pharmacother;88:87-94, 2017 Apr.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Insufficient sialylation can result in rapid clearance of therapeutic glycoproteins by intracellular degradation, which is mainly mediated by asialoglycoprotein receptors (ASGPRs) on hepatic cells. In contrast, for glycoproteins, a long half-life is often related to high level of terminal sialic acid. These could be extremely important for insufficient sialylated biomedicines in clinic, and development of therapeutic glycoproteins in laboratory. However, how the desialylated glycoproteins are removed and how to evaluate the ASGPRs mediated endocytosis in vitro needs further investigate. Herein we described an integrative characterization of ASGPRs in vitro to elucidate its endocytosis properties. The endocytosis was determined by a fluorescence-based quantization method. The results showed that the ASGPRs could bind to poorly sialylated glycoproteins including asialofetuin and low sialylated recombinant Factor VIIa with a relatively higher ASGPRs binding affinity, and induce a more rapid endocytosis in vitro. Moreover, the mechanism under the internalization of ASGPRs was also investigated, which was found to depend on clathrin and caveolin. Utilizing the relative fluorescence quantification can be suitable for measurement of insufficient sialylated glycoprotein endocytosis and quality control of therapeutic glycoproteins, which could be useful for the understanding of the development of therapeutic glycoproteins.
[Mh] Termos MeSH primário: Endocitose
Fluorometria/métodos
Glicoproteínas/metabolismo
Ácido N-Acetilneuramínico/metabolismo
[Mh] Termos MeSH secundário: Animais
Receptor de Asialoglicoproteína
Assialoglicoproteínas/metabolismo
Células CHO
Caveolinas/metabolismo
Clatrina/metabolismo
Cricetinae
Cricetulus
Dinaminas/metabolismo
Endossomos/metabolismo
Fator VIIa/metabolismo
Fetuínas/metabolismo
Fluoresceína-5-Isotiocianato/metabolismo
Fluorescência
Células Hep G2
Seres Humanos
Lisossomos/metabolismo
Ligação Proteica
Proteínas Recombinantes/metabolismo
Reprodutibilidade dos Testes
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Asialoglycoprotein Receptor); 0 (Asialoglycoproteins); 0 (Caveolins); 0 (Clathrin); 0 (Fetuins); 0 (Glycoproteins); 0 (Recombinant Proteins); 0 (asialofetuin); AC71R787OV (recombinant FVIIa); EC 3.4.21.21 (Factor VIIa); EC 3.6.5.5 (Dynamins); GZP2782OP0 (N-Acetylneuraminic Acid); I223NX31W9 (Fluorescein-5-isothiocyanate)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170118
[St] Status:MEDLINE


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[PMID]:27892637
[Au] Autor:Wakasa I; Hayashi M; Abe Y; Suzuki H
[Ad] Endereço:Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
[Ti] Título:Distribution of follicles in canine ovarian tissues and xenotransplantation of cryopreserved ovarian tissues with even distribution of follicles.
[So] Source:Reprod Domest Anim;52 Suppl 2:219-223, 2017 Apr.
[Is] ISSN:1439-0531
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Ovarian follicles are not homogeneously distributed within the ovarian cortex in several species of mammals. Yet to maximize the reproducibility of experimental results of ovarian transplantation, it is essential to assess the degree of density and distribution of follicles in ovarian tissues before their transplantation. In this study, the ovarian cortex from 13 immature bitches (ten purebred and three mongrels) was sectioned into 1.0- to 1.5-mm cubes, those were fixed, sectioned and stained with haematoxylin and eosin. To evaluate the density and distribution of follicles, the mean number of all stages of follicles per square millimetre was calculated after observation under a microscope. The distribution of follicles was considered even when the variance value was lower than 10 or between 10 and 16, with an absolute value of distortion inferior to 1. The mean number of follicles ranged from 3.24 to 28.34/mm in 25 ovaries from 13 bitches examined. The variance and distortion ranged from 0.35 to 119.64 and -1.87 to 4.40, respectively. The distribution of follicles within the ovarian cortex was judged uneven in 12 of 25 ovaries. These results indicated that follicles were not homogeneously distributed within the ovarian cortex in a large proportion of ovaries. In addition, cryopreserved ovarian fragments with even distribution of follicles were transplanted to NSG mice with or without 400 U/kg of disialylated erythropoietin (asialo EPO). After removing both sides of ovary, a piece of ovarian fragment was placed under the kidney capsule in both sides of kidney. At 4 weeks after transplantation, the fragments were recovered from the mice and the number of primordial, primary, secondary and antral follicles was counted. Total number of follicles and survival rates of follicles in transplanted fragments with asialo EPO were higher than without asialo EPO in four bitches examined. These findings suggest that asialo EPO might be effective on the follicular survival of canine ovarian tissues after xenotransplantation. Knowing the degree of density and distribution of follicles in ovarian tissues before transplantation is expected to contribute to the precise interpretation of results after transplantation of the ovarian tissues.
[Mh] Termos MeSH primário: Cães
Folículo Ovariano/anatomia & histologia
Ovário/anatomia & histologia
Ovário/transplante
Transplante Heterólogo/veterinária
[Mh] Termos MeSH secundário: Animais
Assialoglicoproteínas/farmacologia
Criopreservação/veterinária
Eritropoetina/análogos & derivados
Eritropoetina/farmacologia
Feminino
Camundongos
Folículo Ovariano/efeitos dos fármacos
Folículo Ovariano/crescimento & desenvolvimento
Transplante Heterólogo/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Asialoglycoproteins); 0 (asialoerythropoietin); 11096-26-7 (Erythropoietin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161129
[St] Status:MEDLINE
[do] DOI:10.1111/rda.12857


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[PMID]:27832720
[Au] Autor:Sugiyama Y; Katoh T; Honda Y; Gotoh A; Ashida H; Kurihara S; Yamamoto K; Katayama T
[Ad] Endereço:a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Japan.
[Ti] Título:Application study of 1,2-α-l-fucosynthase: introduction of Fucα1-2Gal disaccharide structures on N-glycan, ganglioside, and xyloglucan oligosaccharide.
[So] Source:Biosci Biotechnol Biochem;81(2):283-291, 2017 Feb.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We have recently generated a highly efficient 1,2-α-l-fucosynthase (BbAfcA N423H mutant) by protein engineering of 1,2-α-l-fucosidase from Bifidobacterium bifidum JCM 1254. This synthase could specifically introduce H-antigens (Fucα1-2Gal) into the non-reducing ends of oligosaccharides and in O-linked glycans in mucin glycoprotein. In the present study, we show an extended application of the engineered 1,2-α-l-fucosynthase by demonstrating its ability to insert Fuc residues into N- and O-glycans in fetuin glycoproteins, GM1 ganglioside, and a plant-derived xyloglucan nonasaccharide. This application study broadens the feasibility of this novel H-antigen synthesis technique in functional glycomics.
[Mh] Termos MeSH primário: Dissacarídeos/química
Dissacarídeos/metabolismo
Fucose/química
Gangliosídeos/química
Glucanos/química
Oligossacarídeos/química
Xilanos/química
alfa-L-Fucosidase/metabolismo
[Mh] Termos MeSH secundário: Assialoglicoproteínas/metabolismo
Bifidobacterium/enzimologia
Fetuínas/metabolismo
Fucose/metabolismo
Gangliosídeos/metabolismo
Glucanos/metabolismo
Glicolipídeos/química
Glicolipídeos/metabolismo
Mutação
Oligossacarídeos/metabolismo
Plantas/química
Engenharia de Proteínas
Xilanos/metabolismo
alfa-L-Fucosidase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Asialoglycoproteins); 0 (Disaccharides); 0 (Fetuins); 0 (Gangliosides); 0 (Glucans); 0 (Glycolipids); 0 (Oligosaccharides); 0 (Xylans); 0 (asialofetuin); 28RYY2IV3F (Fucose); 37294-28-3 (xyloglucan); EC 3.2.1.51 (alpha-L-Fucosidase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161112
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2016.1254532


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[PMID]:27667543
[Au] Autor:Lou R; Xie H; Zheng H; Ren Y; Gao M; Guo X; Song Y; Yu W; Liu X; Ma X
[Ad] Endereço:Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China.
[Ti] Título:Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications.
[So] Source:Int J Biol Macromol;93(Pt A):1133-1140, 2016 Dec.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Alginate-galactosylated chitosan/polylysine (AGCP) microcapsules with excellent stability and high permeability were developed and employed in primary hepatocyte applications. The galactosylated chitosan (GC), synthesized via the covalent coupling of lactobionic acid (LA) with low molecular weight and water-soluble chitosan (CS), was ingeniously introduced into the core of alginate microcapsules by regulating the pH of gelling bath. The internal GC of the microcapsules simultaneously provided a large number of binding sites for the hepatocytes and further promoted the hepatocyte-matrix interactions via the recognition of asialoglycoprotein receptors (ASGPRs) on the hepatocyte surface, and afforded the AGCP microcapsules an excellent stability via the electrostatic interactions with alginate. As a consequence, primary hepatocytes in AGCP microcapsules demonstrated enhanced viability, urea synthesis, albumin secretion, and P-450 enzyme activity, showing great prospects for hepatocyte applications in microcapsule system.
[Mh] Termos MeSH primário: Alginatos/química
Alginatos/metabolismo
Quitosana/química
Galactose/química
Hepatócitos/metabolismo
[Mh] Termos MeSH secundário: Alginatos/farmacologia
Animais
Assialoglicoproteínas/metabolismo
Transporte Biológico
Cápsulas
Sobrevivência Celular/efeitos dos fármacos
Ácido Glucurônico/química
Ácido Glucurônico/metabolismo
Ácido Glucurônico/farmacologia
Hepatócitos/citologia
Hepatócitos/efeitos dos fármacos
Ácidos Hexurônicos/química
Ácidos Hexurônicos/metabolismo
Ácidos Hexurônicos/farmacologia
Masculino
Fenômenos Mecânicos
Peso Molecular
Permeabilidade
Polilisina/química
Ratos
Ratos Sprague-Dawley
Solubilidade
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Asialoglycoproteins); 0 (Capsules); 0 (Hexuronic Acids); 059QF0KO0R (Water); 25104-18-1 (Polylysine); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 9012-76-4 (Chitosan); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170322
[Lr] Data última revisão:
170322
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160927
[St] Status:MEDLINE


  5 / 1294 MEDLINE  
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[PMID]:27195678
[Au] Autor:Cabral-Piccin MP; Guillermo LV; Vellozo NS; Filardy AA; Pereira-Marques ST; Rigoni TS; Pereira-Manfro WF; DosReis GA; Lopes MF
[Ad] Endereço:Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, CCS-IBCCF, Ilha do Fundão, Rio de Janeiro, RJ, Brazil.
[Ti] Título:Apoptotic CD8 T-lymphocytes disable macrophage-mediated immunity to Trypanosoma cruzi infection.
[So] Source:Cell Death Dis;7:e2232, 2016 May 19.
[Is] ISSN:2041-4889
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chagas disease is caused by infection with the protozoan Trypanosoma cruzi. CD8 T-lymphocytes help to control infection, but apoptosis of CD8 T cells disrupts immunity and efferocytosis can enhance parasite infection within macrophages. Here, we investigate how apoptosis of activated CD8 T cells affects M1 and M2 macrophage phenotypes. First, we found that CD8 T-lymphocytes and inflammatory monocytes/macrophages infiltrate peritoneum during acute T. cruzi infection. We show that treatment with anti-Fas ligand (FasL) prevents lymphocyte apoptosis, upregulates type-1 responses to parasite antigens, and reduces infection in macrophages cocultured with activated CD8 T cells. Anti-FasL skews mixed M1/M2 macrophage profiles into polarized M1 phenotype, both in vitro and following injection in infected mice. Moreover, inhibition of T-cell apoptosis induces a broad reprogramming of cytokine responses and improves macrophage-mediated immunity to T. cruzi. The results indicate that disposal of apoptotic CD8 T cells increases M2-macrophage differentiation and contributes to parasite persistence.
[Mh] Termos MeSH primário: Linfócitos T CD8-Positivos/imunologia
Doença de Chagas/imunologia
Proteína Ligante Fas/antagonistas & inibidores
Interações Hospedeiro-Parasita
Imunidade Celular/efeitos dos fármacos
Macrófagos/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/farmacologia
Apoptose/efeitos dos fármacos
Assialoglicoproteínas/genética
Assialoglicoproteínas/imunologia
Linfócitos T CD4-Positivos/efeitos dos fármacos
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD4-Positivos/parasitologia
Linfócitos T CD8-Positivos/efeitos dos fármacos
Linfócitos T CD8-Positivos/parasitologia
Comunicação Celular/efeitos dos fármacos
Movimento Celular/efeitos dos fármacos
Doença de Chagas/tratamento farmacológico
Doença de Chagas/genética
Doença de Chagas/parasitologia
Técnicas de Cocultura
Proteína Ligante Fas/genética
Proteína Ligante Fas/imunologia
Regulação da Expressão Gênica
Subunidade p35 da Interleucina-12/genética
Subunidade p35 da Interleucina-12/imunologia
Lectinas Tipo C/genética
Lectinas Tipo C/imunologia
Ativação Linfocitária
Macrófagos/efeitos dos fármacos
Macrófagos/parasitologia
Masculino
Proteínas de Membrana/genética
Proteínas de Membrana/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Fenótipo
Trypanosoma cruzi/crescimento & desenvolvimento
Trypanosoma cruzi/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Asialoglycoproteins); 0 (Fas Ligand Protein); 0 (Fasl protein, mouse); 0 (Interleukin-12 Subunit p35); 0 (Lectins, C-Type); 0 (Membrane Proteins); 0 (Mgl1 protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160520
[St] Status:MEDLINE
[do] DOI:10.1038/cddis.2016.135


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[PMID]:27150510
[Au] Autor:Kidani S; Kaneoka H; Okuzaki Y; Asai S; Kojima Y; Nishijima K; Iijima S
[Ad] Endereço:Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
[Ti] Título:Analyses of chicken sialyltransferases related to O-glycosylation.
[So] Source:J Biosci Bioeng;122(4):379-84, 2016 Oct.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The chicken ß-galactoside α2,3-sialyltransferase 1, 2, and 5 (ST3Gal1, 2, and 5) genes were cloned, and their enzymes were expressed in 293FT cells. ST3Gal1 and 2 exhibited enzymatic activities toward galactose-ß1,3-N-acetylgalactosamine and galactose-ß1,3-N-acetylglucosamine. ST3Gal5 only exhibited activity toward lactosylceramide. ST3Gal1 and 2 and previously cloned ST3Gal3 and 6 transferred CMP-sialic acid to asialofetuin. Reverse-transcription-quantitative PCR indicated that ST3Gal1 was expressed at higher levels in the trachea, lung, spleen, and magnum, and the strong expression of ST3Gal5 was observed in the spleen, magnum, and small and large intestines. ST3Gal1, 5, and 6 were also expressed in the tubular gland cells of the magnum, which secretes egg-white proteins. ST3Gal1, 5, and 6 were expressed in the egg chorioallantoic membrane, in which influenza viruses are propagated for the production of vaccines.
[Mh] Termos MeSH primário: Galinhas/genética
Sialiltransferases/genética
Sialiltransferases/metabolismo
[Mh] Termos MeSH secundário: Acetilgalactosamina/metabolismo
Acetilglucosamina/metabolismo
Animais
Antígenos CD/metabolismo
Assialoglicoproteínas/metabolismo
Linhagem Celular
Membrana Corioalantoide/metabolismo
Proteínas do Ovo/secreção
Fetuínas/metabolismo
Galactose/metabolismo
Glicosilação
Lactosilceramidas/metabolismo
Especificidade de Órgãos
Proteínas Recombinantes/análise
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Sialiltransferases/análise
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Asialoglycoproteins); 0 (Egg Proteins); 0 (Fetuins); 0 (Lactosylceramides); 0 (Recombinant Proteins); 0 (asialofetuin); 4682-48-8 (CDw17 antigen); EC 2.4.99.- (Sialyltransferases); EC 2.4.99.4 (beta-galactoside alpha-2,3-sialyltransferase); KM15WK8O5T (Acetylgalactosamine); V956696549 (Acetylglucosamine); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE


  7 / 1294 MEDLINE  
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[PMID]:26999763
[Au] Autor:Streng-Ouwehand I; Ho NI; Litjens M; Kalay H; Boks MA; Cornelissen LA; Kaur Singh S; Saeland E; Garcia-Vallejo JJ; Ossendorp FA; Unger WW; van Kooyk Y
[Ad] Endereço:Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, Netherlands.
[Ti] Título:Glycan modification of antigen alters its intracellular routing in dendritic cells, promoting priming of T cells.
[So] Source:Elife;5, 2016 Mar 21.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Antigen uptake by dendritic cells and intracellular routing of antigens to specific compartments is regulated by C-type lectin receptors that recognize glycan structures. We show that the modification of Ovalbumin (OVA) with the glycan-structure Lewis(X) (Le(X)) re-directs OVA to the C-type lectin receptor MGL1. Le(X)-modification of OVA favored Th1 skewing of CD4(+) T cells and enhanced cross-priming of CD8(+) T cells. While cross-presentation of native OVA requires high antigen dose and TLR stimuli, Le(X) modification reduces the required amount 100-fold and obviates its dependence on TLR signaling. The OVA-Le(X)-induced enhancement of T cell cross-priming is MGL1-dependent as shown by reduced CD8(+) effector T cell frequencies in MGL1-deficient mice. Moreover, MGL1-mediated cross-presentation of OVA-Le(X) neither required TAP-transporters nor Cathepsin-S and was still observed after prolonged intracellular storage of antigen in Rab11(+)LAMP1(+) compartments. We conclude that controlled neo-glycosylation of antigens can crucially influence intracellular routing of antigens, the nature and strength of immune responses and should be considered for optimizing current vaccination strategies.
[Mh] Termos MeSH primário: Antígenos/química
Antígenos/metabolismo
Células Dendríticas/metabolismo
Ovalbumina/química
Ovalbumina/metabolismo
Polissacarídeos/metabolismo
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Animais
Assialoglicoproteínas/deficiência
Assialoglicoproteínas/metabolismo
Lectinas Tipo C/deficiência
Lectinas Tipo C/metabolismo
Proteínas de Membrana/deficiência
Proteínas de Membrana/metabolismo
Camundongos
Camundongos Knockout
Transporte Proteico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens); 0 (Asialoglycoproteins); 0 (Lectins, C-Type); 0 (Membrane Proteins); 0 (Mgl1 protein, mouse); 0 (Polysaccharides); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160322
[St] Status:MEDLINE


  8 / 1294 MEDLINE  
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[PMID]:26912318
[Au] Autor:Jondle CN; Sharma A; Simonson TJ; Larson B; Mishra BB; Sharma J
[Ad] Endereço:Department of Basic Biomedical Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202.
[Ti] Título:Macrophage Galactose-Type Lectin-1 Deficiency Is Associated with Increased Neutrophilia and Hyperinflammation in Gram-Negative Pneumonia.
[So] Source:J Immunol;196(7):3088-96, 2016 Apr 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:C-type lectin receptors (CLRs), the carbohydrate-recognizing molecules, orchestrate host immune response in homeostasis and in inflammation. In the present study we examined the function of macrophage galactose-type lectin-1 (MGL1), a mammalian CLR, in pneumonic sepsis, a deadly immune disorder frequently associated with a nonresolving hyperinflammation. In a murine model of pneumonic sepsis using pulmonary infection with Klebsiella pneumoniae, the expression of MGL1 was upregulated in the lungs of K. pneumoniae-infected mice, and the deficiency of this CLR in MGL1(-/-) mice resulted in significantly increased mortality to infection than in the MGL1-sufficient wild-type mice, despite a similar bacterial burden. The phagocytic cells from MGL1(-/-) mice did not exhibit any defects in bacterial uptake and intracellular killing and were fully competent in neutrophil extracellular trap formation, a recently identified extracellular killing modality of neutrophils. Instead, the increased susceptibility of MGL1(-/-) mice seemed to correlate with severe lung pathology, indicating that MGL1 is required for resolution of pulmonary inflammation. Indeed, the MGL1(-/-) mice exhibited a hyperinflammatory response, massive pulmonary neutrophilia, and an increase in neutrophil-associated immune mediators. Concomitantly, MGL1-deficient neutrophils exhibited an increased influx in pneumonic lungs of K. pneumoniae-infected mice. Taken together, these results show a previously undetermined role of MGL1 in controlling neutrophilia during pneumonic infection, thus playing an important role in resolution of inflammation. To our knowledge, this is the first study depicting a protective function of MGL1 in an acute pneumonic bacterial infection.
[Mh] Termos MeSH primário: Assialoglicoproteínas/deficiência
Infecções por Bactérias Gram-Negativas/genética
Infecções por Bactérias Gram-Negativas/imunologia
Lectinas Tipo C/deficiência
Macrófagos/imunologia
Proteínas de Membrana/deficiência
Neutrófilos/imunologia
Pneumonia Bacteriana/genética
Pneumonia Bacteriana/imunologia
[Mh] Termos MeSH secundário: Transferência Adotiva
Animais
Modelos Animais de Doenças
Armadilhas Extracelulares/imunologia
Expressão Gênica
Predisposição Genética para Doença
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/mortalidade
Infecções por Bactérias Gram-Negativas/patologia
Mediadores da Inflamação/metabolismo
Klebsiella pneumoniae
Leucocitose/imunologia
Macrófagos/metabolismo
Camundongos
Camundongos Knockout
Infiltração de Neutrófilos/genética
Infiltração de Neutrófilos/imunologia
Neutrófilos/patologia
Fagocitose/genética
Fagocitose/imunologia
Pneumonia Bacteriana/microbiologia
Pneumonia Bacteriana/mortalidade
Pneumonia Bacteriana/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Asialoglycoproteins); 0 (Inflammation Mediators); 0 (Lectins, C-Type); 0 (Membrane Proteins); 0 (Mgl1 protein, mouse)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160226
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1501790


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[PMID]:26568048
[Au] Autor:Bose PP; Mandal G; Kumar D; Duseja A; Chatterjee BP
[Ad] Endereço:Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Hajipur 844102, India. get.ppb@gmail.com ppb.mm@jcbose.ac.in.
[Ti] Título:Visual detection of serum asialohaptoglobin by plasmonic sandwich ELLSA--a new platform for cirrhosis diagnosis.
[So] Source:Analyst;141(1):76-84, 2016 Jan 07.
[Is] ISSN:1364-5528
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The cirrhotic condition of the liver has long been acknowledged as the preface to liver cancer. The desialylation status of the serum acute phase protein, haptoglobin, has been introduced as a new diagnostic analyte for liver cirrhosis. The reliability of this new diagnostic molecule has been evaluated in 30 liver cirrhosis patients having a history of earlier viral hepatitis C (HCV-LC). A novel enzyme linked lectinosorbent assay has been developed coupled with the plasmon mechanism of gold nanoparticle aggregation as the colorimetric read out which can visually distinguish the cirrhotic liver patients from the normal healthy and hepatitis C controls. The assay can be useful for rapid point-of-care detection, and even an untrained person can execute it without a specialized instrument. This method employs Sambucus nigra agglutinin (SNA) to detect the extent of α-2,6 sialylation of serum haptoglobin, the new diagnostic molecule for liver cirrhosis.
[Mh] Termos MeSH primário: Assialoglicoproteínas/sangue
Análise Química do Sangue/métodos
Ensaio de Imunoadsorção Enzimática/métodos
Cirrose Hepática/sangue
Cirrose Hepática/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Feminino
Haptoglobinas
Hepatite C/complicações
Seres Humanos
Cirrose Hepática/complicações
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Asialoglycoproteins); 0 (Haptoglobins); 0 (asialohaptoglobin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151117
[St] Status:MEDLINE
[do] DOI:10.1039/c5an02000j


  10 / 1294 MEDLINE  
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[PMID]:25101832
[Au] Autor:Rohilla R; Garg T; Goyal AK; Rath G
[Ad] Endereço:a Department of Pharmaceutics , ISF College of Pharmacy , Moga , Punjab , India.
[Ti] Título:Herbal and polymeric approaches for liver-targeting drug delivery: novel strategies and their significance.
[So] Source:Drug Deliv;23(5):1645-61, 2016 Jun.
[Is] ISSN:1521-0464
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The liver is a vital organ present in vertebrates, which performs many functions including detoxification, protein synthesis and production of various bio-chemicals which are very important for digestion. A large number of serious liver disorders affect millions of people worldwide which are very difficult to treat properly despite many efforts. There are several factors which are responsible for liver injuries, include plants (Crotalaria Senecio Heliotropium Symphytum officinale), drugs (analgesic and antibiotics), industrial toxins (mercury and lead), water, alcohol and so on. Herbal medicinal preparations can be used for the treatment of a large number of human liver disorders like cirrhosis, hepatitis, carcinomas, etc. Indian Medicinal Practitioner's Co-operative pharmacy and Stores (IMPCPS) approved herbal-based systems (Unani, Siddha and Ayurveda) for the treatment of various chronic liver disorders. Different types of the receptors are found on the surface of hepatocytes, Kupffer cell, hepatic stellate cell and sinusoidal endothelial cells, etc., which can be used for achieving liver targeting. These receptors bind to different types of ligands (galactosylated, lactobionic acid, asialofetuin, etc.) which can be used in the formulation to achieve targeted delivery of the drug. Various novel particulate approaches (liposomes, niosomes, nanoparticles, micelles, nanosuspensions, etc.) can be used to enhance the targeting efficiency of systems to receptors found on the surface of different cells present in the liver. In this review, we focused on the status of liver targeting via herbal and nanotechnology inspired formulation approaches.
[Mh] Termos MeSH primário: Assialoglicoproteínas/metabolismo
Dissacarídeos/metabolismo
Fetuínas/metabolismo
Hepatócitos/efeitos dos fármacos
Hepatopatias/tratamento farmacológico
Fígado/efeitos dos fármacos
Fígado/metabolismo
Nanopartículas/química
Polímeros/metabolismo
Polímeros/farmacologia
[Mh] Termos MeSH secundário: Assialoglicoproteínas/química
Dissacarídeos/química
Sistemas de Liberação de Medicamentos
Fetuínas/química
Hepatócitos/metabolismo
Seres Humanos
Ligantes
Lipossomos
Fígado/química
Nanotecnologia
Polímeros/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Asialoglycoproteins); 0 (Disaccharides); 0 (Fetuins); 0 (Ligands); 0 (Liposomes); 0 (Polymers); 0 (asialofetuin); 65R938S4DV (lactobionic acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140808
[St] Status:MEDLINE
[do] DOI:10.3109/10717544.2014.945018



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