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[PMID]: | 28843521 |
[Au] Autor: | Liu T; Chi H; Chen J; Chen C; Huang Y; Xi H; Xue J; Si Y |
[Ad] Endereço: | Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, China; Department of Pathology, Yale University School of Medicine, New Haven 06520, USA; Shanghai Tenth People's Hospital, Medical School, Tongji University, Sha |
[Ti] Título: | Curcumin suppresses proliferation and in vitro invasion of human prostate cancer stem cells by ceRNA effect of miR-145 and lncRNA-ROR. |
[So] Source: | Gene;631:29-38, 2017 Oct 05. | [Is] ISSN: | 1879-0038 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | Many studies have demonstrated that curcumin can effectively inhibit the proliferation, invasion, and tumorigenesis of prostate cancer cells in vitro and in vivo. In this study, CD44 /CD133 human prostate cancer stem cells (HuPCaSCs) were isolated from the prostate cancer cell lines Du145 and 22RV1. Curcumin treatment of these cells resulted in the inhibition of in vitro proliferation and invasion, and cell cycle arrest. The expression levels of cell cycle proteins (Ccnd1 and Cdk4) and stem cell markers (Oct4, CD44, and CD133) were decreased in curcumin-treated HuPCaSCs. Microarray analysis and northern blotting assays indicated that miR-145 was overexpressed in curcumin-treated HuPCaSCs. Insights of the mechanism of competitive endogenous RNAs (ceRNAs) were gained from bioinformatic analysis, bioinformatics analysis and luciferase activity assays showed that the lncRNA-ROR and Oct4 mRNA both contain miR-145 binding sites, and Oct4 and lncRNA-ROR directly compete for microRNA binding. Curcumin induced high miR-145 expression and inhibited the expression of lncRNA-ROR. The tumorigenicity of curcumin- treated HuPCaSCs in nude mice was significantly reduced. In summary, reducing the expression of endogenous lncRNA-ROR could effectively increase the available concentration of miR-145 in HuPCaSCs, where miR-145 prevents cell proliferation by decreasing Oct4 expression. In particular, we hypothesized that lncRNA-ROR may act as a ceRNA, effectively becoming a sink for miR-145, thereby activating the derepression of core transcription factors Oct4. Thus, curcumin suppresses the proliferation, in vitro invasion, and tumorigenicity of HuPCaSCs through ceRNA effect of miR-145 and lncRNA-ROR caused. |
[Mh] Termos MeSH primário: |
Antineoplásicos Fitogênicos/farmacologia Proliferação Celular/efeitos dos fármacos Curcumina/farmacologia MicroRNAs/metabolismo Neoplasias da Próstata/tratamento farmacológico RNA Longo não Codificante/metabolismo RNA Neoplásico/efeitos dos fármacos
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[Mh] Termos MeSH secundário: |
Antígeno AC133 Animais Ligação Competitiva Biomarcadores/metabolismo Proteínas de Ciclo Celular/metabolismo Linhagem Celular Tumoral Seres Humanos Receptores de Hialuronatos Masculino Camundongos Endogâmicos BALB C Camundongos Nus Invasividade Neoplásica Neoplasias da Próstata/imunologia Ensaios Antitumorais Modelo de Xenoenxerto
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (AC133 Antigen); 0 (Antineoplastic Agents, Phytogenic); 0 (Biomarkers); 0 (Cell Cycle Proteins); 0 (Hyaluronan Receptors); 0 (Linc-RNA-RoR, human); 0 (MIRN145 microRNA, human); 0 (MicroRNAs); 0 (PROM1 protein, human); 0 (RNA, Long Noncoding); 0 (RNA, Neoplasm); IT942ZTH98 (Curcumin) |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 171116 |
[Lr] Data última revisão:
| 171116 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170828 |
[St] Status: | MEDLINE |
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