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[PMID]:28759649
[Au] Autor:Earnest JT; Hantak MP; Li K; McCray PB; Perlman S; Gallagher T
[Ad] Endereço:Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL, United States of America.
[Ti] Título:The tetraspanin CD9 facilitates MERS-coronavirus entry by scaffolding host cell receptors and proteases.
[So] Source:PLoS Pathog;13(7):e1006546, 2017 Jul.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Infection by enveloped coronaviruses (CoVs) initiates with viral spike (S) proteins binding to cellular receptors, and is followed by proteolytic cleavage of receptor-bound S proteins, which prompts S protein-mediated virus-cell membrane fusion. Infection therefore requires close proximity of receptors and proteases. We considered whether tetraspanins, scaffolding proteins known to facilitate CoV infections, hold receptors and proteases together on cell membranes. Using knockout cell lines, we found that the tetraspanin CD9, but not the tetraspanin CD81, formed cell-surface complexes of dipeptidyl peptidase 4 (DPP4), the MERS-CoV receptor, and the type II transmembrane serine protease (TTSP) member TMPRSS2, a CoV-activating protease. This CD9-facilitated condensation of receptors and proteases allowed MERS-CoV pseudoviruses to enter cells rapidly and efficiently. Without CD9, MERS-CoV viruses were not activated by TTSPs, and they trafficked into endosomes to be cleaved much later and less efficiently by cathepsins. Thus, we identified DPP4:CD9:TTSP as the protein complexes necessary for early, efficient MERS-CoV entry. To evaluate the importance of these complexes in an in vivo CoV infection model, we used recombinant Adenovirus 5 (rAd5) vectors to express human DPP4 in mouse lungs, thereby sensitizing the animals to MERS-CoV infection. When the rAd5-hDPP4 vectors co-expressed small RNAs silencing Cd9 or Tmprss2, the animals were significantly less susceptible, indicating that CD9 and TMPRSS2 facilitated robust in vivo MERS-CoV infection of mouse lungs. Furthermore, the S proteins of virulent mouse-adapted MERS-CoVs acquired a CD9-dependent cell entry character, suggesting that CD9 is a selective agent in the evolution of CoV virulence.
[Mh] Termos MeSH primário: Infecções por Coronavirus/metabolismo
Infecções por Coronavirus/virologia
Dipeptidil Peptidase 4/metabolismo
Coronavírus da Síndrome Respiratória do Oriente Médio/fisiologia
Receptores Virais/metabolismo
Serina Endopeptidases/metabolismo
Tetraspanina-29/metabolismo
[Mh] Termos MeSH secundário: Animais
Infecções por Coronavirus/enzimologia
Infecções por Coronavirus/genética
Dipeptidil Peptidase 4/genética
Seres Humanos
Camundongos
Coronavírus da Síndrome Respiratória do Oriente Médio/genética
Receptores Virais/genética
Serina Endopeptidases/genética
Glicoproteína da Espícula de Coronavírus/genética
Glicoproteína da Espícula de Coronavírus/metabolismo
Tetraspanina 28/genética
Tetraspanina 28/metabolismo
Tetraspanina-29/genética
Internalização do Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Virus); 0 (Spike Glycoprotein, Coronavirus); 0 (Tetraspanin 28); 0 (Tetraspanin-29); EC 3.4.14.5 (Dipeptidyl Peptidase 4); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.- (TMPRSS2 protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006546


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[PMID]:28533221
[Au] Autor:Rocha-Perugini V; Martínez Del Hoyo G; González-Granado JM; Ramírez-Huesca M; Zorita V; Rubinstein E; Boucheix C; Sánchez-Madrid F
[Ad] Endereço:Servicio de Inmunología, Hospital de la Princesa, Instituto de Investigación Sanitaria La Princesa, Madrid, Spain vrperugini@externo.cnic.es fsmadrid@salud.madrid.org.
[Ti] Título:CD9 Regulates Major Histocompatibility Complex Class II Trafficking in Monocyte-Derived Dendritic Cells.
[So] Source:Mol Cell Biol;37(15), 2017 Aug 01.
[Is] ISSN:1098-5549
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Antigen presentation by dendritic cells (DCs) stimulates naive CD4 T cells, triggering T cell activation and the adaptive arm of the immune response. Newly synthesized major histocompatibility complex class II (MHC-II) molecules accumulate at MHC-II-enriched endosomal compartments and are transported to the plasma membrane of DCs after binding to antigenic peptides to enable antigen presentation. In DCs, MHC-II molecules are included in tetraspanin-enriched microdomains (TEMs). However, the role of tetraspanin CD9 in these processes remains largely undefined. Here, we show that CD9 regulates the T cell-stimulatory capacity of granulocyte-macrophage colony-stimulating factor (GM-CSF)-dependent bone marrow-derived DCs (BMDCs), without affecting antigen presentation by fms-like tyrosine kinase 3 ligand (Flt3L)-dependent BMDCs. CD9 knockout (KO) GM-CSF-dependent BMDCs, which resemble monocyte-derived DCs (MoDCs), induce lower levels of T cell activation than wild-type DCs, and this effect is related to a reduction in MHC-II surface expression in CD9-deficient MoDCs. Importantly, MHC-II targeting to the plasma membrane is largely impaired in immature CD9 KO MoDCs, in which MHC-II remains arrested in acidic intracellular compartments enriched in LAMP-1 (lysosome-associated membrane protein 1), and MHC-II internalization is also blocked. Moreover, CD9 participates in MHC-II trafficking in mature MoDCs, regulating its endocytosis and recycling. Our results demonstrate that the tetraspanin CD9 specifically regulates antigenic presentation in MoDCs through the regulation of MHC-II intracellular trafficking.
[Mh] Termos MeSH primário: Células Dendríticas/imunologia
Antígenos de Histocompatibilidade Classe II/imunologia
Monócitos/imunologia
Tetraspanina-29/imunologia
[Mh] Termos MeSH secundário: Animais
Apresentação do Antígeno
Linfócitos T CD4-Positivos/imunologia
Movimento Celular
Células Cultivadas
Células Dendríticas/citologia
Células Dendríticas/metabolismo
Deleção de Genes
Fator Estimulador de Colônias de Granulócitos e Macrófagos/imunologia
Antígenos de Histocompatibilidade Classe II/metabolismo
Ativação Linfocitária
Proteínas de Membrana/imunologia
Camundongos Endogâmicos C57BL
Monócitos/citologia
Monócitos/metabolismo
Transporte Proteico
Tetraspanina-29/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histocompatibility Antigens Class II); 0 (Membrane Proteins); 0 (Tetraspanin-29); 0 (flt3 ligand protein); 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE


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[PMID]:28442431
[Au] Autor:Scheltz T; von Bülow J; Beitz E
[Ad] Endereço:Pharmaceutical and Medicinal Chemistry, Christian-Albrechts-University of Kiel, Gutenbergstr. 76, 24118 Kiel, Germany.
[Ti] Título:Reducing isoform complexity of human tetraspanins by optimized expression in Dictyostelium discoideum enables high-throughput functional read-out.
[So] Source:Protein Expr Purif;135:8-15, 2017 Jul.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The human tetraspanin family of scaffold proteins comprises 33 isoforms. Being integral membrane proteins, they organize a so-called tetraspanin web via homomeric and heteromeric protein-protein interactions with integrins, immunoglobulins, growth factors, receptor tyrosine kinases, proteases, signaling proteins, and viral capsid proteins. Tetraspanins promote cellular effects, such as adhesion, migration, invasion, signaling, membrane fusion, protein trafficking, cancer progression, and infections. The ubiquitous expression of multiple tetraspanin isoforms and partner proteins hampers specific interaction studies. Here, we evaluated Dictyostelium discoideum as a non-mammalian expression system for human tetraspanins. Using high-content imaging we quantified tetraspanins in D. discoideum via fusion with green fluorescent protein. Three human tetraspanins, CD9, CD81, and CD151, served as test cases for which optimizations were carried out. We swapped the GFP domain between the N- and C-termini, added a Kozak sequence, and partially or fully adapted of the codon usage. This way, CD81 and CD151 were successfully produced. A conformation specific antibody further confirmed correct folding of CD81 and flow cytometry indicated an intracellular localization. Based on these data, we envision a D. discoideum-based co-expression platform with human partner proteins for studying tetraspanin interactions and their selective druggability on a large scale without the interference of endogenous human proteins.
[Mh] Termos MeSH primário: Dictyostelium/genética
Ensaios de Triagem em Larga Escala
Tetraspanina 24/genética
Tetraspanina 28/genética
Tetraspanina-29/genética
Transgenes
[Mh] Termos MeSH secundário: Animais
Anticorpos/química
Clonagem Molecular
Dictyostelium/metabolismo
Citometria de Fluxo
Expressão Gênica
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Seres Humanos
Conformação Proteica
Dobramento de Proteína
Isoformas de Proteínas/química
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Tetraspanina 24/química
Tetraspanina 24/metabolismo
Tetraspanina 28/química
Tetraspanina 28/metabolismo
Tetraspanina-29/química
Tetraspanina-29/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (CD151 protein, human); 0 (CD81 protein, human); 0 (CD9 protein, human); 0 (Protein Isoforms); 0 (Recombinant Fusion Proteins); 0 (Tetraspanin 24); 0 (Tetraspanin 28); 0 (Tetraspanin-29); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE


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[PMID]:28414312
[Au] Autor:Porpiglia E; Samusik N; Van Ho AT; Cosgrove BD; Mai T; Davis KL; Jager A; Nolan GP; Bendall SC; Fantl WJ; Blau HM
[Ad] Endereço:Blau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.
[Ti] Título:High-resolution myogenic lineage mapping by single-cell mass cytometry.
[So] Source:Nat Cell Biol;19(5):558-567, 2017 May.
[Is] ISSN:1476-4679
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Muscle regeneration is a dynamic process during which cell state and identity change over time. A major roadblock has been a lack of tools to resolve a myogenic progression in vivo. Here we capitalize on a transformative technology, single-cell mass cytometry (CyTOF), to identify in vivo skeletal muscle stem cell and previously unrecognized progenitor populations that precede differentiation. We discovered two cell surface markers, CD9 and CD104, whose combined expression enabled in vivo identification and prospective isolation of stem and progenitor cells. Data analysis using the X-shift algorithm paired with single-cell force-directed layout visualization defined a molecular signature of the activated stem cell state (CD44 /CD98 /MyoD ) and delineated a myogenic trajectory during recovery from acute muscle injury. Our studies uncover the dynamics of skeletal muscle regeneration in vivo and pave the way for the elucidation of the regulatory networks that underlie cell-state transitions in muscle diseases and ageing.
[Mh] Termos MeSH primário: Linhagem da Célula
Separação Celular/métodos
Citometria de Fluxo/métodos
Desenvolvimento Muscular
Músculo Esquelético/metabolismo
Mioblastos Esqueléticos/metabolismo
Regeneração
Análise de Célula Única/métodos
Células-Tronco/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Proliferação Celular
Células Cultivadas
Venenos Elapídicos/toxicidade
Proteína-1 Reguladora de Fusão/metabolismo
Genes Reporter
Genótipo
Ensaios de Triagem em Larga Escala
Receptores de Hialuronatos/metabolismo
Integrina beta4/metabolismo
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Desenvolvimento Muscular/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/lesões
Músculo Esquelético/patologia
Proteína MyoD/metabolismo
Mioblastos Esqueléticos/efeitos dos fármacos
Mioblastos Esqueléticos/patologia
Fator de Transcrição PAX7/deficiência
Fator de Transcrição PAX7/genética
Fenótipo
Regeneração/efeitos dos fármacos
Células-Tronco/efeitos dos fármacos
Células-Tronco/patologia
Tetraspanina-29/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cd44 protein, mouse); 0 (Cd9 protein, mouse); 0 (Elapid Venoms); 0 (Fusion Regulatory Protein-1); 0 (Hyaluronan Receptors); 0 (Integrin beta4); 0 (Luminescent Proteins); 0 (MyoD Protein); 0 (MyoD1 myogenic differentiation protein); 0 (PAX7 Transcription Factor); 0 (Pax7 protein, mouse); 0 (Tetraspanin-29); 37223-96-4 (notexin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1038/ncb3507


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[PMID]:28414289
[Au] Autor:Tamkovich SN; Yunusova NV; Stakheeva МN; Somov AK; Frolova АY; Kirushina NА; Afanasyev SG; Grigoryeva АE; Laktionov PP; Kondakova IV
[Ad] Endereço:Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
[Ti] Título:[Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients].
[Ti] Título:Vydelenie i kharakterizatsiia ékzosom plazmy krovi bol'nykh rakom molochnoi zhelezy i kolorektal'nym rakom..
[So] Source:Biomed Khim;63(2):165-169, 2017 Mar.
[Is] ISSN:2310-6972
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.
[Mh] Termos MeSH primário: Neoplasias da Mama/sangue
Antígeno CD24/sangue
Neoplasias Colorretais/sangue
Exossomos/química
Tetraspanina 28/sangue
Tetraspanina-29/sangue
[Mh] Termos MeSH secundário: Biomarcadores/sangue
Neoplasias da Mama/patologia
Antígeno CD24/genética
Estudos de Casos e Controles
Neoplasias Colorretais/patologia
Feminino
Expressão Gênica
Seres Humanos
Masculino
Meia-Idade
Tetraspanina 28/genética
Tetraspanina-29/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (CD24 Antigen); 0 (CD24 protein, human); 0 (CD81 protein, human); 0 (CD9 protein, human); 0 (Tetraspanin 28); 0 (Tetraspanin-29)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.18097/PBMC20176302165


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[PMID]:28251957
[Au] Autor:Grigor'eva AE; Dyrkheeva NS; Bryzgunova OE; Tamkovich SN; Chelobanov BP; Ryabchikova EI
[Ad] Endereço:Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia.
[Ti] Título:[Contamination of exosome preparations, isolated from biological fluids].
[Ti] Título:Kontaminatsiia preparatov ékzosom, vydelennykh iz biologicheskikh zhidkostei..
[So] Source:Biomed Khim;63(1):91-96, 2017 Jan.
[Is] ISSN:2310-6972
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The aim of our study was to attract the attention of researchers at the problem of contamination of exosome preparations. Using a transmission electron microscope JEM-1400 ("JEOL", Japan) we have examined exosome preparations, isolated according to the conventional scheme of sequential centrifugation from different biological fluids: plasma and urine of healthy persons and patients with oncologic diseases, bovine serum, and culture fluid (MDCK, MDA-MB и MCF-7 cells). All exosome preparations (over 200) contained exosomes, which were identified by immuno-electron microscopy using antibodies to tetraspanins CD63 or CD9. Besides exosomes, all the studied preparations contained contaminating structures: distinct particles of low electron density without limiting membrane ("non-vesicles"). Two main kinds of the "non-vesicles" species were found in exosome preparations: 20-40 nm in size, representing 10-40% of all structures in the preparations; and 40-100 nm in size (identical to exosomes by size). Morphology of the "non-vesicles" allowed to identify them as lipoproteins of intermediate and low density (20-40 nm), and very low density (40-100 nm). The highest level of the contamination was detected in exosome preparations, isolated from blood samples. The results of our study indicate the need to control the composition of exosome preparation by electron microscopy and take into account the presence of contaminating structures in analysis of experimental data.
[Mh] Termos MeSH primário: Adenocarcinoma/química
Artefatos
Neoplasias da Mama/química
Exossomos/metabolismo
Lipoproteínas/química
Neoplasias da Próstata/química
[Mh] Termos MeSH secundário: Adenocarcinoma/sangue
Animais
Biomarcadores/metabolismo
Neoplasias da Mama/sangue
Fracionamento Celular
Micropartículas Derivadas de Células/metabolismo
Micropartículas Derivadas de Células/ultraestrutura
Cães
Exossomos/ultraestrutura
Feminino
Expressão Gênica
Seres Humanos
Lipoproteínas/ultraestrutura
Células MCF-7
Células Madin Darby de Rim Canino
Masculino
Microscopia Eletrônica de Transmissão
Tamanho da Partícula
Neoplasias da Próstata/urina
Tetraspanina 30/genética
Tetraspanina 30/metabolismo
Tetraspanina-29/genética
Tetraspanina-29/metabolismo
Ultracentrifugação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (CD63 protein, human); 0 (CD9 protein, human); 0 (Lipoproteins); 0 (Tetraspanin 30); 0 (Tetraspanin-29)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.18097/PBMC2017630191


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[PMID]:28215843
[Au] Autor:Marcelin G; Ferreira A; Liu Y; Atlan M; Aron-Wisnewsky J; Pelloux V; Botbol Y; Ambrosini M; Fradet M; Rouault C; Hénégar C; Hulot JS; Poitou C; Torcivia A; Nail-Barthelemy R; Bichet JC; Gautier EL; Clément K
[Ad] Endereço:Institute of Cardiometabolism and Nutrition, ICAN, Pitié-Salpêtrière Hospital, F-75013 Paris, France; INSERM, UMRS 1166 (teams 2, 4, and 6 NutriOmics), F-75013 Paris, France; Sorbonne Universités, UPMC Univ Paris 06, UMRS 1166, F-75013 Paris, France. Electronic address: genevieve_marcelin@yahoo.fr.
[Ti] Título:A PDGFRα-Mediated Switch toward CD9 Adipocyte Progenitors Controls Obesity-Induced Adipose Tissue Fibrosis.
[So] Source:Cell Metab;25(3):673-685, 2017 Mar 07.
[Is] ISSN:1932-7420
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Obesity-induced white adipose tissue (WAT) fibrosis is believed to accelerate WAT dysfunction. However, the cellular origin of WAT fibrosis remains unclear. Here, we show that adipocyte platelet-derived growth factor receptor-α-positive (PDGFRα ) progenitors adopt a fibrogenic phenotype in obese mice prone to visceral WAT fibrosis. More specifically, a subset of PDGFRα cells with high CD9 expression (CD9 ) originates pro-fibrotic cells whereas their CD9 counterparts, committed to adipogenesis, are almost completely lost in the fibrotic WAT. PDGFRα pathway activation promotes a phenotypic shift toward PDGFRα CD9 fibrogenic cells, driving pathological remodeling and altering WAT function in obesity. These findings translated to human obesity as the frequency of CD9 progenitors in omental WAT (oWAT) correlates with oWAT fibrosis level, insulin-resistance severity, and type 2 diabetes. Collectively, our data demonstrate that in addition to representing a WAT adipogenic niche, different PDGFRα cell subsets modulate obesity-induced WAT fibrogenesis and are associated with loss of metabolic fitness.
[Mh] Termos MeSH primário: Adipócitos/patologia
Tecido Adiposo/patologia
Obesidade/metabolismo
Obesidade/patologia
Receptor alfa de Fator de Crescimento Derivado de Plaquetas/metabolismo
Células-Tronco/metabolismo
Tetraspanina-29/metabolismo
[Mh] Termos MeSH secundário: Adipogenia
Tecido Adiposo Branco/metabolismo
Tecido Adiposo Branco/patologia
Adulto
Animais
Peso Corporal
Epididimo/metabolismo
Fibrose
Homeostase
Seres Humanos
Resistência à Insulina
Masculino
Camundongos Endogâmicos C57BL
Obesidade/fisiopatologia
Fator de Crescimento Derivado de Plaquetas/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Platelet-Derived Growth Factor); 0 (Tetraspanin-29); EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


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[PMID]:28188731
[Au] Autor:Dohutia C; Chetia D; Gogoi K; Sarma K
[Ad] Endereço:Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh 786004, India. Electronic address: chnadrajit@gmail.com.
[Ti] Título:Design, in silico and in vitro evaluation of curcumin analogues against Plasmodium falciparum.
[So] Source:Exp Parasitol;175:51-58, 2017 Apr.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The polyphenolic compound curcumin has been reported for its antimalarial properties in various scientific studies. Plasmodium falciparum ATP6, the parasite orthologue of mammalian sarcoplasmic Ca ATPase (SERCA) has been identified as a key molecular target of both artemisinin and curcumin. The work was thereby undertaken to study the anti-malarial properties of two different series of curcumin analogues based on their docking interactions with PfATP6 and correlating the results with their anti-malarial activity. The compounds were designed retaining similar functional groups as that of the parent curcumin nucleus while incorporating changes in the carbon chain length, unsaturated groups and the number of ketone groups. The compounds (1E, 4E)-1,5-bis(4-methylphenyl)penta-1,4-dien-3-one (CD-9), (1E, 4E)-1,5-bis(4-methoxyphenyl)penta-1,4-dien-3-one (CD-8) and (E)-1,3-bis(4-hydroxylphenyl)prop-2-en-1-one (CD-1) showed IC values of 1.642 µM, 1.764 µM and 2.59 µM in 3D7 strain and 3.039 µM, 7.40 µM and 11.3 µM in RKL-2 strain respectively. Detailed structure-activity relationship studies of the compounds showed that CD-9 and CD-8 had a common hydrophobic interaction with the residue Leu268 of the PfATP6 protein and has been postulated through our study to be the reason for their antimalarial activity as seen after corroborating the results with the in vitro study. The study provided valuable insight about the ligand-protein interaction of the various functional groups of curcumin and its analogues against the PfATP6 protein and their importance in imparting antimalarial action.
[Mh] Termos MeSH primário: Antimaláricos/farmacologia
Curcumina/análogos & derivados
Curcumina/farmacologia
Plasmodium falciparum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Acetofenonas/química
Antígenos CD1/metabolismo
Benzaldeídos/química
Antígenos CD8/metabolismo
Chalcona/análogos & derivados
Avaliação Pré-Clínica de Medicamentos
Concentração Inibidora 50
Ligantes
Simulação de Acoplamento Molecular
Pentanonas/química
Tetraspanina-29/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetophenones); 0 (Antigens, CD1); 0 (Antimalarials); 0 (Benzaldehydes); 0 (CD8 Antigens); 0 (Ligands); 0 (Pentanones); 0 (Tetraspanin-29); 5S5A2Q39HX (Chalcone); 9QXO7BCY9L (dibenzylidene acetone); IT942ZTH98 (Curcumin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE


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[PMID]:28160150
[Au] Autor:Miyado K; Kang W; Yamatoya K; Hanai M; Nakamura A; Mori T; Miyado M; Kawano N
[Ad] Endereço:Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan. miyado-k@ncchd.go.jp.
[Ti] Título:Exosomes versus microexosomes: Shared components but distinct functions.
[So] Source:J Plant Res;130(3):479-483, 2017 May.
[Is] ISSN:1618-0860
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:In multicellular organisms, cellular components are constantly translocated within cells and are also transported exclusively between limited cells, regardless of their physical distance. Exosomes function as one of the key mediators of intercellular transportation. External vesicles were identified 50 years ago in plants and now reconsidered to be exosome-like vesicles. Meanwhile, a well-known exosomal component, tetraspanin CD9, regulates sperm-egg fusion in mammals. A number of Arabidopsis tetraspanins are also expressed in reproductive tissues at fertilization, and are localized at the plasma membrane of protoplasts. Moreover, CD9-containing structures (or 'microexosomes') are released from mouse eggs during their maturation and promote the sperm-egg fusion. This phenomenon implies that two types of shared-component intercellular carriers might be released from multiple types of plant and animal cells, which widely regulate biological phenomena. We herein highlight their discrete structures, formation processes, and functions.
[Mh] Termos MeSH primário: Exossomos/metabolismo
Exossomos/fisiologia
Fertilização/fisiologia
Interações Espermatozoide-Óvulo/fisiologia
Tetraspaninas/metabolismo
Tetraspaninas/fisiologia
[Mh] Termos MeSH secundário: Animais
Arabidopsis/fisiologia
Membrana Celular/fisiologia
Masculino
Fusão de Membrana/fisiologia
Camundongos
Oócitos/fisiologia
Fenômenos Fisiológicos Vegetais
Plantas
Vesículas Secretórias
Espermatozoides/fisiologia
Tetraspanina-29/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Tetraspanin-29); 0 (Tetraspanins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE
[do] DOI:10.1007/s10265-017-0907-7


  10 / 742 MEDLINE  
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[PMID]:28129113
[Au] Autor:Nishida-Aoki N; Tominaga N; Takeshita F; Sonoda H; Yoshioka Y; Ochiya T
[Ad] Endereço:Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, 5-1-1, Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.
[Ti] Título:Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic Strategy against Cancer Metastasis.
[So] Source:Mol Ther;25(1):181-191, 2017 Jan 04.
[Is] ISSN:1525-0024
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Metastasis is the main cause of cancer mortality for many types of cancer; however, difficulties remain in effectively preventing metastasis. It has been recently and widely reported that cancer-derived extracellular vesicles (EVs) contribute to cancer metastasis. Thus, therapeutic strategies targeting cancer-derived EVs hold great promise because of the possibility of EVs driving the cancer microenvironment toward metastasis. Here, we provide a novel strategy for therapeutic antibody treatment to target cancer-derived EVs and inhibit the metastasis of breast cancer in a mouse model, establishing a rationale for further clinical investigation. Treatment with human-specific anti-CD9 or anti-CD63 antibodies significantly decreased metastasis to the lungs, lymph nodes, and thoracic cavity, although no obvious effects on primary xenograft tumor growths were observed. In in vitro and in vivo experiments, the EVs incubated with the targeted antibodies were preferentially internalized by macrophages, suggesting that antibody-tagged cancer-derived EVs would be eliminated by macrophages. Our results suggested that therapeutic antibody administration effectively suppresses EV-triggered metastasis in cancer and that the removal of EVs could be a novel strategy for cancer therapy.
[Mh] Termos MeSH primário: Antineoplásicos
Vesículas Extracelulares/metabolismo
Neoplasias/metabolismo
Neoplasias/patologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/farmacologia
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Micropartículas Derivadas de Células/metabolismo
Modelos Animais de Doenças
Vesículas Extracelulares/imunologia
Seres Humanos
Macrófagos/efeitos dos fármacos
Macrófagos/imunologia
Macrófagos/metabolismo
Camundongos
Metástase Neoplásica
Neoplasias/imunologia
Neoplasias/terapia
Fagocitose
Tetraspanina 30/imunologia
Tetraspanina 30/metabolismo
Tetraspanina-29/imunologia
Tetraspanina-29/metabolismo
Carga Tumoral/efeitos dos fármacos
Carga Tumoral/imunologia
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antineoplastic Agents); 0 (Tetraspanin 30); 0 (Tetraspanin-29)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE



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