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[PMID]:29371677
[Au] Autor:An Z; Sabalic M; Bloomquist RF; Fowler TE; Streelman T; Sharpe PT
[Ad] Endereço:Centre for Craniofacial and Regenerative Biology, Dental Institute, Kings College London, London, SE1 9RT, UK.
[Ti] Título:A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.
[So] Source:Nat Commun;9(1):378, 2018 01 25.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The extent to which heterogeneity within mesenchymal stem cell (MSC) populations is related to function is not understood. Using the archetypal MSC in vitro surface marker, CD90/Thy1, here we show that 30% of the MSCs in the continuously growing mouse incisor express CD90/Thy1 and these cells give rise to 30% of the differentiated cell progeny during postnatal development. In adulthood, when growth rate homeostasis is established, the CD90/Thy1 MSCs decrease dramatically in number. When adult incisors are cut, the growth rate increases to rapidly re-establish tooth length and homeostasis. This accelerated growth rate correlates with the re-appearance of CD90/Thy MSCs and re-establishment of their contribution to cell differentiation. A population of Celsr1 quiescent cells becomes mitotic following clipping and replenishes the CD90/Thy1 population. A sub-population of MSCs thus exists in the mouse incisor, distinguished by expression of CD90/Thy1 that plays a specific role only during periods of increased growth rate.
[Mh] Termos MeSH primário: Linhagem da Célula/genética
Incisivo/citologia
Células Mesenquimais Estromais/citologia
Osteogênese/genética
Antígenos Thy-1/genética
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Contagem de Células
Diferenciação Celular
Proliferação Celular
Citometria de Fluxo
Expressão Gênica
Incisivo/crescimento & desenvolvimento
Incisivo/metabolismo
Células Mesenquimais Estromais/metabolismo
Camundongos
Camundongos Transgênicos
Mitose
Receptores Acoplados a Proteínas-G/genética
Receptores Acoplados a Proteínas-G/metabolismo
Antígenos Thy-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Celsr1 protein, mouse); 0 (Receptors, G-Protein-Coupled); 0 (Thy-1 Antigens)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02785-6


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[PMID]:28454682
[Au] Autor:Priglinger E; Wurzer C; Steffenhagen C; Maier J; Hofer V; Peterbauer A; Nuernberger S; Redl H; Wolbank S; Sandhofer M
[Ad] Endereço:AUVA Research Center, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Linz, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria. Electronic address: Eleni.Priglinger@trauma.lbg.ac.at.
[Ti] Título:The adipose tissue-derived stromal vascular fraction cells from lipedema patients: Are they different?
[So] Source:Cytotherapy;19(7):849-860, 2017 07.
[Is] ISSN:1477-2566
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AIMS: Lipedema is a hormone-related disease of women characterized by enlargement of the extremities caused by subcutaneous deposition of adipose tissue. In healthy patients application of autologous adipose tissue-derived cells has shown great potential in several clinical studies for engrafting of soft tissue reconstruction in recent decades. The majority of these studies have used the stromal vascular fraction (SVF), a heterogeneous cell population containing adipose-derived stromal/stem cells (ASC), among others. Because cell identity and regenerative properties might be affected by the health condition of patients, we characterized the SVF cells of 30 lipedema patients in comparison to 22 healthy patients. METHODS: SVF cells were analyzed regarding cell yield, viability, adenosine triphosphate content, colony forming units and proliferative capacity, as well as surface marker profile and differentiation potential in vitro. RESULTS: Our results demonstrated a significantly enhanced SVF cell yield isolated from lipedema compared with healthy patients. In contrast, the adipogenic differentiation potential of SVF cells isolated from lipedema patients was significantly reduced compared with healthy patients. Interestingly, expression of the mesenchymal marker CD90 and the endothelial/pericytic marker CD146 was significantly enhanced when isolated from lipedema patients. DISCUSSION: The enhanced number of CD90 and CD146 cells could explain the increased cell yield because the other tested surface marker were not reduced in lipedema patients. Because the cellular mechanism and composition in lipedema is largely unknown, our findings might contribute to a better understanding of its etiology.
[Mh] Termos MeSH primário: Tecido Adiposo/patologia
Lipedema/patologia
Células Estromais/citologia
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Adipogenia/fisiologia
Tecido Adiposo/citologia
Adulto
Antígeno CD146/metabolismo
Estudos de Casos e Controles
Diferenciação Celular
Células Cultivadas
Feminino
Seres Humanos
Meia-Idade
Células-Tronco/citologia
Células-Tronco/patologia
Células Estromais/metabolismo
Células Estromais/patologia
Antígenos Thy-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD146 Antigen); 0 (MCAM protein, human); 0 (Thy-1 Antigens); 8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:28957409
[Au] Autor:Moreau JM; Cen S; Berger A; Furlonger C; Paige CJ
[Ad] Endereço:Princess Margaret Cancer Centre, University Health Network, Toronto, Canada.
[Ti] Título:Bone marrow basophils provide survival signals to immature B cells in vitro but are dispensable in vivo.
[So] Source:PLoS One;12(9):e0185509, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Immature B cells are the first B cell progenitors to express a fully formed B cell receptor and are therefore subject to extensive selection processes that act to mitigate the emergence of autoreactive clones. While it is well appreciated that most B cell generation in the bone marrow is highly dependent on access to molecules present in the local milieu, the existence of extrinsically provided factors that modulate immature B cell biology is ambiguous. Nonetheless, a population of CD49b+CD90lo cells has demonstrated in vitro potential to promote immature B cell survival. Using a mouse basophil reporter strain we confirmed the identity of these CD49b+CD90lo supportive cells as basophils. However, analysis of bone marrow B cell populations following lineage specific basophil depletion demonstrates that basophils do not have a significant role in vivo in modulating immature B cell biology during steady-state conditions.
[Mh] Termos MeSH primário: Basófilos/citologia
Células da Medula Óssea/citologia
Células Precursoras de Linfócitos B/citologia
[Mh] Termos MeSH secundário: Animais
Basófilos/metabolismo
Células da Medula Óssea/metabolismo
Linhagem da Célula
Sobrevivência Celular
Técnicas de Cocultura
Citoproteção
Feminino
Proteínas de Homeodomínio/metabolismo
Cadeias lambda de Imunoglobulina/metabolismo
Integrina alfa2/metabolismo
Contagem de Linfócitos
Camundongos
Células Precursoras de Linfócitos B/metabolismo
Antígenos Thy-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Immunoglobulin lambda-Chains); 0 (Integrin alpha2); 0 (Thy-1 Antigens); 128559-51-3 (RAG-1 protein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185509


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[PMID]:28758259
[Au] Autor:Ngoc Tran TD; Stovall KE; Suantawee T; Hu Y; Yao S; Yang LJ; Adisakwattana S; Cheng H
[Ad] Endereço:Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
[Ti] Título:Transient receptor potential melastatin 4 channel is required for rat dental pulp stem cell proliferation and survival.
[So] Source:Cell Prolif;50(5), 2017 Oct.
[Is] ISSN:1365-2184
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Investigate the role of the transient receptor potential melastatin 4 (TRPM4) channel in rat dental pulp stem cell (DPSC) proliferation and survival. MATERIALS AND METHODS: Immunofluorescence and FACS analysis were used to detect the stem cell marker CD90. Alizarin Red S and Oil Red O staining were used to identify osteoblast and adipocyte differentiation, respectively. To characterize TRPM4, patch-clamp recordings were obtained from single cells in the whole-cell configuration mode. The significance of TRPM4 for proliferation and survival was examined with 9-phenanthrol, a TRPM4 inhibitor during a 96-hour period of culture. Real-time Ca imaging analysis with Fura-2AM was used to investigate the impact of TRPM4 on intracellular Ca signals. RESULTS: DPSCs were CD90-positive and differentiated into osteoblasts. Patch-clamp recordings revealed currents typical of TRPM4 that were Ca -activated, voltage-dependent and Na -conducting. Inhibition of TRPM4 resulted in a significant reduction in the cell population after a 96-hr period of culture and transformed the biphasic pattern of intracellular Ca signalling into sustained oscillations. CONCLUSIONS: Rat DPSCs have stem cell characteristics and functional TRPM4 channels that are required for proliferation and survival. These data suggest that the shape and frequency of intracellular Ca signals may mediate stem cell proliferation and survival.
[Mh] Termos MeSH primário: Adipócitos/citologia
Proliferação Celular
Polpa Dentária/citologia
Osteoblastos/citologia
Células-Tronco/citologia
Canais de Cátion TRPM/metabolismo
[Mh] Termos MeSH secundário: Adipócitos/metabolismo
Adipogenia
Animais
Sinalização do Cálcio
Diferenciação Celular
Sobrevivência Celular
Células Cultivadas
Polpa Dentária/metabolismo
Osteoblastos/metabolismo
Osteogênese
Ratos
Células-Tronco/metabolismo
Antígenos Thy-1/análise
Antígenos Thy-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (TRPM Cation Channels); 0 (TRPM4 protein, rat); 0 (Thy-1 Antigens)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1111/cpr.12360


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[PMID]:28749943
[Au] Autor:Moussy A; Cosette J; Parmentier R; da Silva C; Corre G; Richard A; Gandrillon O; Stockholm D; Páldi A
[Ad] Endereço:Ecole Pratique des Hautes Etudes, PSL Research University, UMRS 951, INSERM, Univ-Evry, Evry, France.
[Ti] Título:Integrated time-lapse and single-cell transcription studies highlight the variable and dynamic nature of human hematopoietic cell fate commitment.
[So] Source:PLoS Biol;15(7):e2001867, 2017 Jul.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Individual cells take lineage commitment decisions in a way that is not necessarily uniform. We address this issue by characterising transcriptional changes in cord blood-derived CD34+ cells at the single-cell level and integrating data with cell division history and morphological changes determined by time-lapse microscopy. We show that major transcriptional changes leading to a multilineage-primed gene expression state occur very rapidly during the first cell cycle. One of the 2 stable lineage-primed patterns emerges gradually in each cell with variable timing. Some cells reach a stable morphology and molecular phenotype by the end of the first cell cycle and transmit it clonally. Others fluctuate between the 2 phenotypes over several cell cycles. Our analysis highlights the dynamic nature and variable timing of cell fate commitment in hematopoietic cells, links the gene expression pattern to cell morphology, and identifies a new category of cells with fluctuating phenotypic characteristics, demonstrating the complexity of the fate decision process (which is different from a simple binary switch between 2 options, as it is usually envisioned).
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Células-Tronco Hematopoéticas/metabolismo
Células-Tronco Multipotentes/metabolismo
Transcrição Genética
[Mh] Termos MeSH secundário: Antígeno AC133/genética
Antígeno AC133/metabolismo
Antígenos CD34/genética
Antígenos CD34/metabolismo
Biomarcadores/metabolismo
Diferenciação Celular
Linhagem da Célula
Proliferação Celular
Forma Celular
Rastreamento de Células
Células Cultivadas
Sangue Fetal/citologia
Perfilação da Expressão Gênica
Células-Tronco Hematopoéticas/citologia
Seres Humanos
Processamento de Imagem Assistida por Computador
Microscopia Confocal
Células-Tronco Multipotentes/citologia
Análise de Componente Principal
Análise de Célula Única
Antígenos Thy-1/genética
Antígenos Thy-1/metabolismo
Imagem com Lapso de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AC133 Antigen); 0 (Antigens, CD34); 0 (Biomarkers); 0 (PROM1 protein, human); 0 (Thy-1 Antigens)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2001867


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[PMID]:28687660
[Au] Autor:Resende M; Cardoso MS; Ribeiro AR; Flórido M; Borges M; Castro AG; Alves NL; Cooper AM; Appelberg R
[Ad] Endereço:IBMC - Instituto de Biologia Molecular e Celular and i3S - Instituto de Investigação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal; mrsilva@ibmc.up.pt.
[Ti] Título:Innate IFN-γ-Producing Cells Developing in the Absence of IL-2 Receptor Common γ-Chain.
[So] Source:J Immunol;199(4):1429-1439, 2017 Aug 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:IFN-γ is known to be predominantly produced by lymphoid cells such as certain subsets of T cells, NK cells, and other group 1 innate lymphoid cells. In this study, we used IFN-γ reporter mouse models to search for additional cells capable of secreting this cytokine. We identified a novel and rare population of nonconventional IFN-γ-producing cells of hematopoietic origin that were characterized by the expression of Thy1.2 and the lack of lymphoid, myeloid, and NK lineage markers. The expression of IFN-γ by this population was higher in the liver and lower in the spleen. Furthermore, these cells were present in mice lacking both the and the common γ-chain (γc) genes (Rag2 γc ), indicating their innate nature and their γc cytokine independence. Rag2 γc mice are as resistant to as Rag2 mice, whereas Rag2 mice lacking IFN-γ are more susceptible than either or Rag2 γc These lineage-negative CD45 /Thy1.2 cells are found within the mycobacterially induced granulomatous structure in the livers of infected Rag2 γc animals and are adjacent to macrophages that expressed inducible NO synthase, suggesting a potential protective role for these IFN-γ-producing cells. Accordingly, Thy1.2-specific mAb administration to infected Rag2 γc animals increased growth in the liver. Overall, our results demonstrate that a population of Thy1.2 non-NK innate-like cells present in the liver expresses IFN-γ and can confer protection against infection in immunocompromised mice.
[Mh] Termos MeSH primário: Células-Tronco Hematopoéticas/imunologia
Imunidade Inata
Interferon gama/biossíntese
Interferon gama/genética
Subunidade gama Comum de Receptores de Interleucina/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/administração & dosagem
Proteínas de Ligação a DNA/deficiência
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/imunologia
Granuloma/imunologia
Granuloma/microbiologia
Hospedeiro Imunocomprometido/imunologia
Interferon gama/imunologia
Subunidade gama Comum de Receptores de Interleucina/deficiência
Subunidade gama Comum de Receptores de Interleucina/genética
Células Matadoras Naturais/imunologia
Antígenos Comuns de Leucócito/genética
Antígenos Comuns de Leucócito/imunologia
Fígado/citologia
Fígado/imunologia
Fígado/microbiologia
Macrófagos/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Mycobacterium avium/crescimento & desenvolvimento
Mycobacterium avium/imunologia
Óxido Nítrico Sintase Tipo II/biossíntese
Óxido Nítrico Sintase Tipo II/genética
Óxido Nítrico Sintase Tipo II/imunologia
Baço/citologia
Baço/imunologia
Antígenos Thy-1/genética
Antígenos Thy-1/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (DNA-Binding Proteins); 0 (Interleukin Receptor Common gamma Subunit); 0 (Rag2 protein, mouse); 0 (Thy-1 Antigens); 82115-62-6 (Interferon-gamma); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, mouse); EC 3.1.3.48 (Leukocyte Common Antigens); EC 3.1.3.48 (Ptprc protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601701


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[PMID]:28645561
[Au] Autor:Liu Q; Chen K; Liu Z; Huang Y; Zhao R; Wei L; Yu X; He J; Liu J; Qi J; Qin Y; Li B
[Ad] Endereço:Division of Liver Transplantation, Department of Surgery, West China Hospital, Sichuan University, Chengdu 610041, China; Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
[Ti] Título:BORIS up-regulates OCT4 via histone methylation to promote cancer stem cell-like properties in human liver cancer cells.
[So] Source:Cancer Lett;403:165-174, 2017 Sep 10.
[Is] ISSN:1872-7980
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Accumulating evidence has revealed the importance of cancer stem cells (CSCs) in chemoresistance and recurrence. BORIS, a testes-specific CTCF paralog, has been shown to be associated with stemness traits of embryonic cancer cells and epithelial CSCs. We previously reported that BORIS is correlated with the expression of the CSC marker CD90 in hepatocellular carcinoma (HCC). These results encourage us to wonder whether BORIS exerts functions on CSC-like traits of human liver cancer cells. Here, we report that BORIS was enriched in HCC tissues. Exogenous overexpression of BORIS promoted CSC-like properties, including self-renewal, chemoresistance, migration and invasion in Huh7 and HCCLM3 cells. Conversely, BORIS knockdown suppressed CSC-like properties in SMMC-7721 and HepG2 cells and inhibited tumorigenicity in SMMC-7721 cells. Moreover, BORIS alteration did not affect the DNA methylation status of the minimal promoter and exon 1 region of OCT4. However, BORIS overexpression enhanced the amount of BORIS bound on the OCT4 promoter and increased H3K4me2, while reducing H3K27me3; BORIS depletion decreased BORIS and H3K4me2 on the OCT4 promoter, while increasing H3K27me3. These results revealed that BORIS is associated with the CSC-like traits of human liver cancer cells through the epigenetic regulation of OCT4.
[Mh] Termos MeSH primário: Carcinoma Hepatocelular/metabolismo
Proteínas de Ligação a DNA/metabolismo
Histonas/metabolismo
Neoplasias Hepáticas/metabolismo
Células-Tronco Neoplásicas/metabolismo
Fator 3 de Transcrição de Octâmero/metabolismo
[Mh] Termos MeSH secundário: Animais
Antibióticos Antineoplásicos/farmacologia
Sítios de Ligação
Carcinoma Hepatocelular/tratamento farmacológico
Carcinoma Hepatocelular/genética
Carcinoma Hepatocelular/patologia
Movimento Celular
Proliferação Celular
Autorrenovação Celular
Metilação de DNA
Proteínas de Ligação a DNA/genética
Doxorrubicina/farmacologia
Resistência a Medicamentos Antineoplásicos
Epigênese Genética
Regulação Neoplásica da Expressão Gênica
Células Hep G2
Seres Humanos
Neoplasias Hepáticas/tratamento farmacológico
Neoplasias Hepáticas/genética
Neoplasias Hepáticas/patologia
Metilação
Camundongos Nus
Invasividade Neoplásica
Células-Tronco Neoplásicas/patologia
Fator 3 de Transcrição de Octâmero/genética
Fenótipo
Regiões Promotoras Genéticas
Ligação Proteica
Proteínas Proto-Oncogênicas c-myc/genética
Proteínas Proto-Oncogênicas c-myc/metabolismo
Interferência de RNA
Fatores de Transcrição SOXB1/genética
Fatores de Transcrição SOXB1/metabolismo
Transdução de Sinais
Antígenos Thy-1/genética
Antígenos Thy-1/metabolismo
Fatores de Tempo
Transfecção
Carga Tumoral
Regulação para Cima
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (CTCFL protein, human); 0 (DNA-Binding Proteins); 0 (Histones); 0 (MYC protein, human); 0 (Octamer Transcription Factor-3); 0 (POU5F1 protein, human); 0 (Proto-Oncogene Proteins c-myc); 0 (SOX2 protein, human); 0 (SOXB1 Transcription Factors); 0 (Thy-1 Antigens); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE


  8 / 2974 MEDLINE  
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[PMID]:28445714
[Au] Autor:Stivers NS; Pelisch N; Orem BC; Williams J; Nally JM; Stirling DP
[Ad] Endereço:Kentucky Spinal Cord Injury Research Center and Departments of Neurological Surgery, Microbiology and Immunology, Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY, USA.
[Ti] Título:The toll-like receptor 2 agonist Pam3CSK4 is neuroprotective after spinal cord injury.
[So] Source:Exp Neurol;294:1-11, 2017 Aug.
[Is] ISSN:1090-2430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microglia/macrophage activation and recruitment following spinal cord injury (SCI) is associated with both detrimental and reparative functions. Stimulation of the innate immune receptor Toll-like receptor-2 (TLR2) has shown to be beneficial following SCI, and it increases axonal regeneration following optic nerve crush. However, the mechanism(s) remain unclear. As microglia express high levels of TLR2, we hypothesized that modulating the microglial response to injury using a specific TLR2 agonist, Pam3CSK4, would prevent secondary-mediated white matter degeneration following SCI. To test this hypothesis, we documented acute changes in microglia, axons, and oligodendroglia over time using two-photon excitation and an ex vivo laser-induced SCI (LiSCI) model. We utilized double transgenic mice that express GFP in either microglia or oligodendroglia, and YFP in axons, and we applied the lipophilic fluorescent dye (Nile Red) to visualize myelin. We found that treatment with Pam3CSK4 initiated one hour after injury induced a significant increase in the extent and timing of the microglial response to injury compared to vehicle controls. This enhanced response was observed 2 to 4h following SCI and was most prominent in areas closer to the ablation site. In addition, Pam3CSK4 treatment significantly reduced axonal dieback rostral and caudal to the ablation at 6h post-SCI. This protective effect of Pam3CSK4 was also mirrored when assessing secondary bystander axonal damage (i.e., axons spared by the primary injury that then succumb to secondary degeneration), and when assessing the survival of oligodendroglia. Following these imaging experiments, custom microarray analysis of the ex vivo spinal cord preparations revealed that Pam3CSK4-treatment induced an alternative (mixed M1:M2) microglial activation profile. In summary, our data suggest that by providing a second "sterile" activation signal to microglia through TLR2/TLR1 signaling, the microglial response to injury can be modulated in situ and is highly neuroprotective.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica/efeitos dos fármacos
Lipopeptídeos/farmacologia
Lipopeptídeos/uso terapêutico
Degeneração Neural/tratamento farmacológico
Traumatismos da Medula Espinal/tratamento farmacológico
[Mh] Termos MeSH secundário: 2',3'-Nucleotídeo Cíclico Fosfodiesterases/genética
2',3'-Nucleotídeo Cíclico Fosfodiesterases/metabolismo
Animais
Axônios/efeitos dos fármacos
Axônios/patologia
Receptor 1 de Quimiocina CX3C
Citocinas/metabolismo
Modelos Animais de Doenças
Regulação da Expressão Gênica/genética
Inflamação/tratamento farmacológico
Inflamação/etiologia
Inflamação/metabolismo
Terapia a Laser/efeitos adversos
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Ativação de Macrófagos
Glicoproteínas de Membrana/metabolismo
Camundongos
Camundongos Transgênicos
Microglia/efeitos dos fármacos
Degeneração Neural/etiologia
Fármacos Neuroprotetores/farmacologia
Fármacos Neuroprotetores/uso terapêutico
Receptores de Superfície Celular/metabolismo
Receptores de Quimiocinas/genética
Receptores de Quimiocinas/metabolismo
Traumatismos da Medula Espinal/complicações
Traumatismos da Medula Espinal/etiologia
Traumatismos da Medula Espinal/patologia
Antígenos Thy-1/genética
Antígenos Thy-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CX3C Chemokine Receptor 1); 0 (Cx3cr1 protein, mouse); 0 (Cytokines); 0 (Lipopeptides); 0 (Luminescent Proteins); 0 (MRC1 protein, mouse); 0 (Membrane Glycoproteins); 0 (Neuroprotective Agents); 0 (Pam(3)CSK(4) peptide); 0 (Receptors, Cell Surface); 0 (Receptors, Chemokine); 0 (Thy-1 Antigens); EC 3.1.4.- (2',3'-Cyclic-Nucleotide Phosphodiesterases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE


  9 / 2974 MEDLINE  
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[PMID]:28339035
[Au] Autor:Xue L; Li Y; Chen J
[Ad] Endereço:Department of Urology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710014, P.R. China.
[Ti] Título:Duration of simulated microgravity affects the differentiation of mesenchymal stem cells.
[So] Source:Mol Med Rep;15(5):3011-3018, 2017 May.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Previous evidence has suggested that physical microenvironments and mechanical stresses, independent of soluble factors, influence mesenchymal stem cell (MSC) fate. In the present study, simulated microgravity (SMG) was demonstrated to regulate the differentiation of mesenchymal stem cells. This may be a novel strategy for tissue engineering and regenerative medicine. Rat MSCs were cultured for 72 h or 10 days in either normal gravity or a clinostat to model microgravity, followed with culture in diverse differential media. A short period of stimulation (72 h) promoted MSCs to undergo endothelial, neuronal and adipogenic differentiation. In comparison, extended microgravity (10 days) promoted MSCs to differentiate into osteoblasts. A short period of exposure to SMG significantly decreased ras homolog family member A (RhoA) activity. However, RhoA activity significantly increased following prolonged exposure to SMG. When RhoA activity was inhibited, the effects of prolonged exposure to SMG were reversed. These results demonstrated that the duration of SMG regulates the differentiation fate of MSCs via the RhoA­associated pathway.
[Mh] Termos MeSH primário: Células Mesenquimais Estromais/citologia
Simulação de Ausência de Peso/métodos
[Mh] Termos MeSH secundário: Adipócitos/citologia
Adipogenia
Animais
Diferenciação Celular/imunologia
Diferenciação Celular/fisiologia
Células Cultivadas
Citoesqueleto/fisiologia
Receptores de Hialuronatos/metabolismo
Masculino
Neurônios/citologia
Osteoblastos/metabolismo
Osteogênese/fisiologia
Ratos
Ratos Wistar
Antígenos Thy-1/metabolismo
Fatores de Tempo
Engenharia Tecidual/métodos
Ausência de Peso
Proteína rhoA de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hyaluronan Receptors); 0 (Thy-1 Antigens); EC 3.6.5.2 (rhoA GTP-Binding Protein)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2017.6357


  10 / 2974 MEDLINE  
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[PMID]:28320452
[Au] Autor:Pu L; Meng M; Wu J; Zhang J; Hou Z; Gao H; Xu H; Liu B; Tang W; Jiang L; Li Y
[Ad] Endereço:Department of Cardiovascular Surgery, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, 245, East of Renmin Road, Kunming, 650051, Yunnan, People's Republic of China.
[Ti] Título:Compared to the amniotic membrane, Wharton's jelly may be a more suitable source of mesenchymal stem cells for cardiovascular tissue engineering and clinical regeneration.
[So] Source:Stem Cell Res Ther;8(1):72, 2017 Mar 21.
[Is] ISSN:1757-6512
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The success of developing cardiovascular tissue engineering (CTE) grafts greatly needs a readily available cell substitute for endothelial and interstitial cells. Perinatal annexes have been proposed as a valuable source of mesenchymal stem cells (MSCs) for tissue engineering and regenerative medicine. The objective of the present study is to evaluate the potential of human Wharton's jelly MSCs (WJ-MSCs) and amniotic membrane MSCs (AM-MSCs) as a seeding cell in CTE and cardiovascular regenerative medicine. METHODS: WJ-MSCs/AM-MSCs were isolated and characterized in vitro according to their morphology, proliferation, self-renewal, phenotype, and multipotency. More importantly, the characteristics of hemocompatibility, extracellular matrix deposition, and gene expression and viability of both MSCs were investigated. RESULTS: Fibroblast-like human WJ-MSCs and AM-MSCs were successfully isolated and positively expressed the characteristic markers CD73, CD90, and CD105 but were negative for CD34, CD45, and HLA-DR. Both MSCs shared trilineage differentiation toward the adipogenic, osteogenic, and chondrogenic lineages. The proliferative and self-renewal capacity of WJ-MSCs was significantly higher than that of AM-MSCs (P < 0.001). WJ-MSCs provided comparable properties of antiplatelet adhesion and did not activate the coagulation cascade to endothelial cells. However, aggregated platelets were visualized on the surface of AM-MSCs-derived cell sheets and the intrinsic pathway was activated. Furthermore, WJ-MSCs have superior properties of collagen deposition and higher viability than AM-MSCs during cell sheet formation. CONCLUSIONS: This study highlights that WJ-MSCs could act as a functional substitute of endothelial and interstitial cells, which could serve as an appealing and practical single-cell source for CTE and regenerative therapy.
[Mh] Termos MeSH primário: Âmnio/citologia
Sistema Cardiovascular/citologia
Células Mesenquimais Estromais/citologia
Engenharia Tecidual
Geleia de Wharton/citologia
[Mh] Termos MeSH secundário: 5'-Nucleotidase/genética
5'-Nucleotidase/metabolismo
Adipócitos/citologia
Adipócitos/metabolismo
Âmnio/metabolismo
Biomarcadores/metabolismo
Sistema Cardiovascular/metabolismo
Comunicação Celular
Diferenciação Celular
Proliferação Celular
Separação Celular
Condrócitos/citologia
Condrócitos/metabolismo
Endoglina/genética
Endoglina/metabolismo
Matriz Extracelular/química
Matriz Extracelular/metabolismo
Feminino
Proteínas Ligadas por GPI/genética
Proteínas Ligadas por GPI/metabolismo
Expressão Gênica
Seres Humanos
Células Mesenquimais Estromais/metabolismo
Osteoblastos/citologia
Osteoblastos/metabolismo
Ativação Plaquetária
Agregação Plaquetária
Gravidez
Antígenos Thy-1/genética
Antígenos Thy-1/metabolismo
Geleia de Wharton/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (ENG protein, human); 0 (Endoglin); 0 (GPI-Linked Proteins); 0 (Thy-1 Antigens); EC 3.1.3.5 (5'-Nucleotidase); EC 3.1.3.5 (NT5E protein, human)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1186/s13287-017-0501-x



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