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[PMID]: 28483911 [Au] Autor: Lee KJ; Kim Y; Yoo YH; Kim MS; Lee SH; Kim CG; Park K; Jeoung D; Lee H; Ko IY; Hahn JH [Ad] Endereço: Department of Anatomy and Cell Biology, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea. [Ti] Título: CD99-Derived Agonist Ligands Inhibit Fibronectin-Induced Activation of ß1 Integrin through the Protein Kinase A/SHP2/Extracellular Signal-Regulated Kinase/PTPN12/Focal Adhesion Kinase Signaling Pathway. [So] Source: Mol Cell Biol;37(14), 2017 Jul 15. [Is] ISSN: 1098-5549 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The human CD99 protein is a 32-kDa glycosylated transmembrane protein that regulates various cellular responses, including cell adhesion and leukocyte extravasation. We previously reported that CD99 activation suppresses ß1 integrin activity through dephosphorylation of focal adhesion kinase (FAK) at Y397. We explored a molecular mechanism underlying the suppression of ß1 integrin activity by CD99 agonists and its relevance to tumor growth CD99-Fc fusion proteins or a series of CD99-derived peptides suppressed ß1 integrin activity by specifically interacting with three conserved motifs of the CD99 extracellular domain. CD99CRIII3, a representative CD99-derived 3-mer peptide, facilitated protein kinase A-SHP2 interaction and subsequent activation of the HRAS/RAF1/MEK/ERK signaling pathway. Subsequently, CD99CRIII3 induced FAK phosphorylation at S910, which led to the recruitment of PTPN12 and PIN1 to FAK, followed by FAK dephosphorylation at Y397. Taken together, these results indicate that CD99-derived agonist ligands inhibit fibronectin-mediated ß1 integrin activation through the SHP2/ERK/PTPN12/FAK signaling pathway. [Mh] Termos MeSH primário: Adesão Celular/fisiologia Movimento Celular/fisiologia Fibronectinas/metabolismo Proteína-Tirosina Quinases de Adesão Focal/metabolismo Cadeias beta de Integrinas/metabolismo Transdução de Sinais [Mh] Termos MeSH secundário: Antígeno 12E7/metabolismo Linhagem Celular Tumoral Proteínas Quinases Dependentes de AMP Cíclico/metabolismo MAP Quinases Reguladas por Sinal Extracelular/metabolismo Seres Humanos Ligantes Fosforilação Proteína Tirosina Fosfatase não Receptora Tipo 11/metabolismo Proteína Tirosina Fosfatase não Receptora Tipo 12/metabolismo Transdução de Sinais/fisiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); 0 (CD99 protein, human); 0 (Fibronectins); 0 (Integrin beta Chains); 0 (Ligands); EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 3.1.3.48 (PTPN12 protein, human); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 12) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170510 [St] Status: MEDLINE

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[PMID]: 28420430 [Au] Autor: Husak Z; Dworzak MN [Ad] Endereço: St. Anna Kinderkrebsforschung, Children's Cancer Research Institute, Zimmermannplatz 10, 1090, Vienna, Austria. zvenyslava.husak@ccri.at. [Ti] Título: Chronic stress induces CD99, suppresses autophagy, and affects spontaneous adipogenesis in human bone marrow stromal cells. [So] Source: Stem Cell Res Ther;8(1):83, 2017 Apr 18. [Is] ISSN: 1757-6512 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Bone marrow-derived mesenchymal stromal cells (MSCs) are multipotent cells with a high constitutive level of autophagy and low expression of CD99. Under certain conditions, MSCs may develop tumorigenic properties. However, these transformation-induced conditions are largely unknown. Recently, we have identified an association between Hsp70, a main participant in cellular stress response and tumorigenesis, and CD99. Preliminary observations had revealed upregulation of both proteins in stressed long-term cultured MSCs. And so we hypothesized that CD99 is implicated in stress-induced mechanisms of cellular transformation in MSCs. Hence, we investigated the effects of prolonged stress on MSCs and the role of CD99 and autophagy in their survival. METHODS: Human telomerase reverse transcriptase (hTERT) overexpressing immortalized MSCs and primary bone marrow stromal cells were used to investigate the influence of long-term serum deprivation and hypoxia on growth and differentiation of MSCs. Cell proliferation and apoptosis were evaluated using flow cytometry, differentiation capabilities of MSCs were assessed by immunohistochemical staining followed by microscopic examination. CD99, Hsp70 expression were analyzed using flow cytometry, western blotting, and reverse transcriptase polymerase chain reaction. Autophagy was explored with specific inhibitors using cell morphology examination and western blotting. RESULTS: Chronic stress factors are able to change the morphology of MSCs and to inhibit spontaneous differentiation into adipocyte lineage. Furthermore, CD99 elevation and downregulation of p53 and p21 accompanied defective autophagy, which is usually associated with tumor formation. We found that inhibition of autophagy by chloroquine promoted cell detachment and modulated CD99 expression level whereas incorporation of CD99 recombinant protein into the cells suppressed autophagy. CONCLUSIONS: Obtained results provide a model for chronic stress-induced transformation of MSCs via CD99 and may therefore be highly relevant to mesenchymal tumorigenesis. [Mh] Termos MeSH primário: Adipócitos/citologia Autofagia Células da Medula Óssea/metabolismo Diferenciação Celular Células Mesenquimais Estromais/metabolismo Estresse Fisiológico [Mh] Termos MeSH secundário: Antígeno 12E7/genética Antígeno 12E7/metabolismo Adipócitos/metabolismo Apoptose Células da Medula Óssea/citologia Hipóxia Celular Linhagem Celular Proliferação Celular Proteínas de Choque Térmico HSP70/genética Proteínas de Choque Térmico HSP70/metabolismo Seres Humanos Células Mesenquimais Estromais/citologia Telomerase/genética Telomerase/metabolismo Proteína Supressora de Tumor p53/genética Proteína Supressora de Tumor p53/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); 0 (HSP70 Heat-Shock Proteins); 0 (Tumor Suppressor Protein p53); EC 2.7.7.49 (TERT protein, human); EC 2.7.7.49 (Telomerase) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170420 [St] Status: MEDLINE [do] DOI: 10.1186/s13287-017-0532-3

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[PMID]: 28397481 [Au] Autor: Touni I; Tabll AA; Sobieh SS; Hewedy MA; Abd YS; Viazov S [Ti] Título: Diagnosis of Hepatitis C Virus Infection Using a Novel Mouse Monoclonal Antibody to Detect Serum HCV E1/E2 Antigens. [So] Source: Clin Lab;63(4):669-678, 2017 Apr 01. [Is] ISSN: 1433-6510 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: BACKGROUND: Hepatitis C virus (HCV) is a major cause of liver disease worldwide and in Egypt. The aim of this study was to detect HCV E1/E2 antigens using a novel mouse monoclonal antibody (mAb) designated (7G9) as a diagnostic and alternative approach for HCV detection. METHODS: The detection of HCV-E1/E2 antigens in 138 patients positive for HCV infection tested by RT-PCR and 25 healthy individuals negative for HCV as control group was done by an optimized in-house ELISA and DotELISA (based on the molecular mimicry of E2 to immunoglobulins). RESULTS: The mAb (7G9) was found to be IgM (heavy-chain)/kappa (light-chain) and characterization by western blot revealed two bands at 63 kDa for E2 and 31 kDa for E1. ELISA peptide mapping showed high reactivity with peptide derived from HCV E1 (a.a. 315 - 323) and low reactivity to peptides derived from HCV E2 (a.a. 517 - 531) and HCV E2 (a.a. 412 - 419). The mAb (7G9) showed no reactivity with HBV Ag, S. typhi or B. Abortus Ag proving high specificity. AUC for HCV-E1/E2 detection was 0.96 for all HCV patients with sensitivity 87% (119/137), specificity 88% (22/25) and efficiency 87%. The HCV-E1/E2 antigens detection by Dot-ELISA showed 76.8% sensitivity, 88% specificity and the efficiency of the assay was 78.5%. Furthermore, no correlation was found between serum HCV viral load and HCV E1/E2 antigens detection. CONCLUSIONS: The ELISA and Dot-ELISA based on the monoclonal antibody (7G9) are reliable, rapid, easy and economic diagnostic assays for HCV infection. [Mh] Termos MeSH primário: Hepatite C [Mh] Termos MeSH secundário: Antígeno 12E7 Animais Egito Hepacivirus Anticorpos Anti-Hepatite C Seres Humanos Camundongos Proteínas do Envelope Viral [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); 0 (Hepatitis C Antibodies); 0 (Viral Envelope Proteins) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170412 [St] Status: MEDLINE [do] DOI: 10.7754/Clin.Lab.2016.161124

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[PMID]: 28392342 [Au] Autor: Takheaw N; Laopajon W; Chuensirikulchai K; Kasinrerk W; Pata S [Ad] Endereço: Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. [Ti] Título: Exploitation of human CD99 expressing mouse myeloma cells as immunogen for production of mouse specific polyclonal antibodies. [So] Source: Protein Expr Purif;134:82-88, 2017 Jun. [Is] ISSN: 1096-0279 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In this study, we describe the application of a molecular biology technique for the production of mouse polyclonal antibodies (pAbs) specific to human cell surface molecules. Production of the pAb specific to the human CD99 surface molecule was used as the study model. The retroviral expression system was employed to generate human CD99 expressing mouse myeloma cells. After cell sorting and single cell cloning, a myeloma clone which stably expressed high levels of human CD99 on its surface was established. The human CD99 expressing mouse myeloma cells were then used as the immunogen for immunization of BALB/c mice. As endogenous proteins of mouse myeloma cells possess self-non-immunogenicity for BALB/c mice, after immunization, only the expressed human CD99 molecules induce antibody response. After three immunizations, high titers of mouse anti-CD99 pAbs were successfully produced. The produced pAb specifically reacted to both recombinant human CD99 and native CD99 molecules expressed on human blood cells. The established technology is simple and valuable for the production of pAbs specific to human CD99 membrane proteins which can be used for characterization of the CD99 molecule. [Mh] Termos MeSH primário: Antígeno 12E7 Anticorpos Monoclonais Murinos/imunologia Imunização Mieloma Múltiplo [Mh] Termos MeSH secundário: Antígeno 12E7/biossíntese Antígeno 12E7/genética Antígeno 12E7/imunologia Animais Seres Humanos Camundongos Camundongos Endogâmicos BALB C Mieloma Múltiplo/genética Mieloma Múltiplo/imunologia Mieloma Múltiplo/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); 0 (Antibodies, Monoclonal, Murine-Derived); 0 (CD99 protein, human) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170411 [St] Status: MEDLINE

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[PMID]: 28376588 [Au] Autor: Ding ZY; Wang YF; Wang X; Rao Q [Ad] Endereço: Department of Pathology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China. [Ti] Título: [Expression and significance of STAT6 in solitary fibrous tumor]. [So] Source: Zhonghua Bing Li Xue Za Zhi;46(4):235-239, 2017 Apr 08. [Is] ISSN: 0529-5807 [Cp] País de publicação: China [La] Idioma: chi [Ab] Resumo: To study the clinicopathologic features, the differential diagnosis and the expression of STAT6 in solitary fibrous tumor (SFT). Eighty cases of SFT were evaluated. The expression of STAT6, CD34, CD99 and bcl-2 protein was studied in these cases and in other groups of soft tissue tumors by immunohistochemical EnVision method. The results were analyzed and relevant literature were reviewed. The expression rate of STAT6 in SFT was 97.5% (78/80) and that in other soft tissue tumors was 3.3% (3/90). The difference was significant ( <0.05). The expression rates of CD34, CD99 and bcl-2 were 88.8% (71/80), 76.3% (61/80) and 75.0% (60/80) in SFT, respectively, which were significantly different from STAT6 expression rate ( <0.05). The expression of STAT6 in SFT has high sensitivity (97.5%) and specificity (96.7%). The expression of STAT6 in SFT is higher than that of CD34, CD99 and bcl-2. STAT6 may be a useful marker for clinical diagnosis of SFT. [Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo Fator de Transcrição STAT6/metabolismo Neoplasias de Tecidos Moles/metabolismo Tumores Fibrosos Solitários/metabolismo [Mh] Termos MeSH secundário: Antígeno 12E7/metabolismo Antígenos CD34/metabolismo Diagnóstico Diferencial Feminino Seres Humanos Imuno-Histoquímica Masculino Proteínas Proto-Oncogênicas c-bcl-2/metabolismo Sensibilidade e Especificidade Neoplasias de Tecidos Moles/diagnóstico Tumores Fibrosos Solitários/diagnóstico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); 0 (Antigens, CD34); 0 (Biomarkers, Tumor); 0 (CD99 protein, human); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (STAT6 Transcription Factor); 0 (STAT6 protein, human) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170406 [St] Status: MEDLINE [do] DOI: 10.3760/cma.j.issn.0529-5807.2017.04.004

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[PMID]: 28003343 [Au] Autor: Bedau T; Peters F; Prox J; Arnold P; Schmidt F; Finkernagel M; Köllmann S; Wichert R; Otte A; Ohler A; Stirnberg M; Lucius R; Koudelka T; Tholey A; Biasin V; Pietrzik CU; Kwapiszewska G; Becker-Pauly C [Ad] Endereço: Unit for Degradomics of the Protease Web, Institute of Biochemistry, University of Kiel, Kiel, Germany. [Ti] Título: Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin ß and promotes transendothelial cell migration. [So] Source: FASEB J;31(3):1226-1237, 2017 Mar. [Is] ISSN: 1530-6860 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The adhesion molecule CD99 is essential for the transendothelial migration of leukocytes. In this study, we used biochemical and cellular assays to show that CD99 undergoes ectodomain shedding by the metalloprotease meprin ß and subsequent intramembrane proteolysis by γ-secretase. The cleavage site in CD99 was identified by mass spectrometry within an acidic region highly conserved through different vertebrate species. This finding fits perfectly to the unique cleavage specificity of meprin ß with a strong preference for aspartate residues and suggests coevolution of protease and substrate. We hypothesized that limited CD99 cleavage by meprin ß would alter cellular transendothelial migration (TEM) behavior in tissue remodeling processes, such as inflammation and cancer. Indeed, meprin ß induced cell migration of Lewis lung carcinoma cells in an TEM assay. Accordingly, deficiency of meprin ß in mice resulted in significantly increased CD99 protein levels in the lung. Therefore, meprin ß could serve as a therapeutic target, given that in a proof-of-concept approach we showed accumulation of CD99 protein in lungs of meprin ß inhibitor-treated mice.-Bedau, T., Peters, F., Prox, J., Arnold, P., Schmidt, F., Finkernagel, M., Köllmann, S., Wichert, R., Otte, A., Ohler, A., Stirnberg, M., Lucius, R., Koudelka, T., Tholey, A., Biasin, V., Pietrzik, C. U., Kwapiszewska, G., Becker-Pauly, C. Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin ß and promotes transendothelial cell migration. [Mh] Termos MeSH primário: Antígeno 12E7/metabolismo Sequência Conservada Metaloendopeptidases/metabolismo Proteólise Migração Transendotelial e Transepitelial [Mh] Termos MeSH secundário: Antígeno 12E7/química Animais Carcinoma Pulmonar de Lewis/metabolismo Células HEK293 Células HeLa Seres Humanos Camundongos Camundongos Endogâmicos C57BL [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); EC 3.4.24.- (Metalloendopeptidases); EC 3.4.24.18 (meprin A) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161223 [St] Status: MEDLINE [do] DOI: 10.1096/fj.201601113R

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[PMID]: 27866429 [Au] Autor: Zhou G; Yang W; Yu L; Yu T; Liu Z [Ad] Endereço: a Department of Gastroenterology , The Shanghai Tenth People's Hospital, Tongji University , Shanghai , China. [Ti] Título: CD99 refers to the activity of inflammatory bowel disease. [So] Source: Scand J Gastroenterol;52(3):359-364, 2017 Mar. [Is] ISSN: 1502-7708 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Inflammatory bowel disease (IBD), composed of Crohn's disease (CD) and ulcerative colitis (UC), is an inflammatory autoimmune disease. CD99 has been reported to participate in migration of leukocytes and T cell activation. However, the roles of CD99 in IBD are obscure. MATERIALS AND METHODS: CD99 expression was examined in peripheral blood mononuclear cells (PBMCs) and inflamed mucosa from IBD patients by qRT-PCR. Serum TNF-α and IL-17A levels were detected by ELISA. Correlations of CD99 expression with TNF-α, IL-17A, Crohn's disease activity index (CDAI), simple endoscopic score for CD (SES-CD), Mayo index, and Truelove grading were performed by Pearson's correlation. RESULTS: CD99 expression was increased in PBMCs and inflamed mucosa from active CD and UC patients, and CD99 expression was also increased in the inflamed mucosa compared with unaffected control from the same patients. Serum TNF-α and IL-17A levels were increased in active CD or UC patients, and positively correlated with CD99 expression in PBMCs (CD: r = .402, p = .009; r = .350, p = .025. UC: r = .289, p = .028; r = .322, p = .014). Moreover, CD99 expression in inflamed mucosa was correlated with CDAI, SES-CD, Mayo index, and Truelove grading (r = .410, p = .012; r = .341, p = .005; r = .366, p = .002; r = .312, p = .011). CONCLUSION: CD99 expression is increased in patients with active IBD, and positively correlated with disease activity. Therefore, CD99 expression can be used as an index to evaluate the activity of IBD. [Mh] Termos MeSH primário: Antígeno 12E7/metabolismo Doenças Inflamatórias Intestinais/metabolismo Interleucina-17/sangue Fator de Necrose Tumoral alfa/sangue [Mh] Termos MeSH secundário: Antígeno 12E7/genética Adolescente Adulto Estudos de Casos e Controles China Ensaio de Imunoadsorção Enzimática Feminino Seres Humanos Doenças Inflamatórias Intestinais/sangue Doenças Inflamatórias Intestinais/genética Mucosa Intestinal/metabolismo Leucócitos Mononucleares/metabolismo Masculino Meia-Idade Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); 0 (CD99 protein, human); 0 (Interleukin-17); 0 (Tumor Necrosis Factor-alpha) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161122 [St] Status: MEDLINE [do] DOI: 10.1080/00365521.2016.1256426

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[PMID]: 27500883 [Au] Autor: Ke Ch; Duan Q; Yang H; Zhu F; Yan M; Xu SP; Zhou S; Wan F; Shu K; Lei T; Xia LM [Ad] Endereço: Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China. [Ti] Título: Meningeal Ewing Sarcoma/Peripheral PNET: Clinicopathological, Immunohistochemical and FISH study of four cases. [So] Source: Neuropathology;37(1):35-44, 2017 02. [Is] ISSN: 1440-1789 [Cp] País de publicação: Australia [La] Idioma: eng [Ab] Resumo: Meningeal Ewing Sarcoma (ES)/peripheral primitive neuroectodermal tumor (pPNET) is a rare diagnostically challenging small round cell tumor in the CNS. This study investigates the clinical pathological features of four cases of this tumor from archives of 6 years in our hospital. Patients were within the median age of 21.5 years and male to female ratio was 1:1. The tumors distributed at the supra-tentorial location, posterior fossa and lumbar vertebral canal, usually presenting as the dura-sited nodule or having close connection with the meninges within the cranium or vertebral canal. Histopathologically, small round undifferentiated tumor cells with hypercellularities, scant cytoplasm and inconspicuous nucleoli were observed, although some components such as atypical larger vesicular nuclei, prominent nucleoli of tumor cells, necrotic foci and mesenchymal collagen proliferation forming the lobular structure, were also appreciated. Immunohistochemally, tumor cells displayed membranous positivity of CD99 (4/4), nuclear positivity of FLI-1 (4/4) and NKX2.2 (4/4), negativity of EMA, GFAP and synaptophysin expression. The histochemical PAS staining showed weak positivity in one case. Fluorescence in situ hybridization (FISH) test using EWSR1 (22q12) dual color break apart rearrangement probe showed positive results in two cases. Results suggest that using a panel of immunohistochemical markers, including NKX2.2, CD99, FLI-1, EMA, GFAP and synaptophysin, combined with the supplementary EWSR1 FISH test, helps to define the diagnosis of meningeal ES/pPNET of CNS. [Mh] Termos MeSH primário: Neoplasias Meníngeas/diagnóstico Tumores Neuroectodérmicos Primitivos Periféricos/diagnóstico Sarcoma de Ewing/diagnóstico [Mh] Termos MeSH secundário: Antígeno 12E7/metabolismo Adolescente Adulto Biomarcadores Tumorais/genética Biomarcadores Tumorais/metabolismo Proteínas de Ligação a Calmodulina/genética Proteínas de Ligação a Calmodulina/metabolismo Dura-Máter/metabolismo Dura-Máter/patologia Feminino Proteínas de Homeodomínio/metabolismo Seres Humanos Imuno-Histoquímica Hibridização in Situ Fluorescente Masculino Neoplasias Meníngeas/genética Neoplasias Meníngeas/metabolismo Neoplasias Meníngeas/patologia Tumores Neuroectodérmicos Primitivos Periféricos/genética Tumores Neuroectodérmicos Primitivos Periféricos/metabolismo Tumores Neuroectodérmicos Primitivos Periféricos/patologia Proteína Proto-Oncogênica c-fli-1/metabolismo Proteína EWS de Ligação a RNA Proteínas de Ligação a RNA/genética Proteínas de Ligação a RNA/metabolismo Sarcoma de Ewing/genética Sarcoma de Ewing/metabolismo Sarcoma de Ewing/patologia Fatores de Transcrição/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); 0 (Biomarkers, Tumor); 0 (Calmodulin-Binding Proteins); 0 (EWSR1 protein, human); 0 (FLI1 protein, human); 0 (Homeodomain Proteins); 0 (Nkx-2.2 homedomain protein); 0 (Proto-Oncogene Protein c-fli-1); 0 (RNA-Binding Protein EWS); 0 (RNA-Binding Proteins); 0 (Transcription Factors) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160809 [St] Status: MEDLINE [do] DOI: 10.1111/neup.12325

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[PMID]: 27482634 [Au] Autor: Hery AL; Ornvold K; Memoli V; Bridge J; Linos K [Ad] Endereço: Department of Pathology, Dartmouth-Hitchcock Medical Center, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA. [Ti] Título: A case of CIC-rearranged undifferentiated round-cell sarcoma with exclusive spindled morphology and diffuse CD99 positivity: a potential pitfall. [So] Source: Histopathology;70(2):314-316, 2017 Jan. [Is] ISSN: 1365-2559 [Cp] País de publicação: England [La] Idioma: eng [Mh] Termos MeSH primário: Antígeno 12E7/biossíntese Proteínas Repressoras/genética Sarcoma de Células Pequenas/diagnóstico [Mh] Termos MeSH secundário: Antígeno 12E7/análise Biomarcadores Tumorais/análise Criança Erros de Diagnóstico Feminino Seres Humanos Hibridização in Situ Fluorescente Recidiva Local de Neoplasia Sarcoma de Ewing/diagnóstico Sarcoma de Ewing/genética Sarcoma de Ewing/patologia Sarcoma de Células Pequenas/genética Sarcoma de Células Pequenas/patologia [Pt] Tipo de publicação: CASE REPORTS; LETTER [Nm] Nome de substância: 0 (12E7 Antigen); 0 (Biomarkers, Tumor); 0 (CD99 protein, human); 0 (CIC protein, human); 0 (Repressor Proteins) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160803 [St] Status: MEDLINE [do] DOI: 10.1111/his.13051

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[PMID]: 27764235 [Au] Autor: Cox CV; Diamanti P; Moppett JP; Blair A [Ad] Endereço: Bristol Institute for Transfusion Sciences, NHS Blood and Transplant, Filton, Bristol, United Kingdom. [Ti] Título: Investigating CD99 Expression in Leukemia Propagating Cells in Childhood T Cell Acute Lymphoblastic Leukemia. [So] Source: PLoS One;11(10):e0165210, 2016. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A significant number of children with T-lineage acute lymphoblastic leukemia (T-ALL) fail to respond to therapy and experience early relapse. CD99 has been shown to be overexpressed on T-ALL cells and is considered to be a reliable detector of the disease. However, the relevance of CD99 overexpression in ALL has not been investigated in a functional context. The aim of this study was to investigate the functional capacity of CD99+ cells in childhood ALL and determine the suitability of CD99 as a therapeutic target. Flow cytometric analyses confirmed higher expression of CD99 in ALL blasts (81.5±22.7%) compared to normal hemopoietic stem cells (27.5±21.9%) and T cells (3.1±5.2%, P≤0.004). When ALL cells were sorted and assessed in functional assays, all 4 subpopulations (CD34+/CD99+, CD34+/CD99-, CD34-/CD99+ and CD34-/CD99-) could proliferate in vitro and establish leukemia in NSG mice. Leukemia propagating cell frequencies ranged from 1 in 300 to 1 in 7.4x104 but were highest in the CD34+/CD99- subpopulation. In addition, all four subpopulations had self-renewal ability in secondary NSG mice. Cells in each subpopulation contained patient specific TCR rearrangements and karyotypic changes that were preserved with passage through serial NSG transplants. Despite high levels of CD99 antigen on the majority of blast cells, leukemia initiating capacity in vivo was not restricted to cells that express this protein. Consequently, targeting CD99 alone would not eliminate all T-ALL cells with the ability to maintain the disease. The challenge remains to develop therapeutic strategies that can eliminate all leukemia cells with self-renewal capacity in vivo. [Mh] Termos MeSH primário: Antígeno 12E7/metabolismo Leucemia-Linfoma Linfoblástico de Células T Precursoras/patologia [Mh] Termos MeSH secundário: Adolescente Animais Antígenos CD34/metabolismo Células da Medula Óssea/citologia Células da Medula Óssea/metabolismo Proliferação Celular Células Cultivadas Criança Pré-Escolar Feminino Citometria de Fluxo Células-Tronco Hematopoéticas/citologia Células-Tronco Hematopoéticas/metabolismo Seres Humanos Hibridização in Situ Fluorescente Cariótipo Masculino Camundongos Camundongos Endogâmicos NOD Leucemia-Linfoma Linfoblástico de Células T Precursoras/diagnóstico Leucemia-Linfoma Linfoblástico de Células T Precursoras/metabolismo Receptores de Antígenos de Linfócitos T/genética Receptores de Antígenos de Linfócitos T/metabolismo Linfócitos T/citologia Linfócitos T/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (12E7 Antigen); 0 (Antigens, CD34); 0 (Receptors, Antigen, T-Cell) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161021 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0165210

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