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Pesquisa : D12.776.395.550.200.175 [Categoria DeCS]
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[PMID]:28259931
[Au] Autor:Tu M; Cai L; Zheng W; Su Z; Chen Y; Qi S
[Ad] Endereço:Department of Neurosurgery, NanFang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
[Ti] Título:CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma.
[So] Source:Mol Med Rep;15(4):1713-1721, 2017 Apr.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Cluster of differentiation 164 (CD164), a sialomucin, has been demonstrated to be involved in the regulation of proliferation, apoptosis, adhesion and differentiation in multiple cancers. CD164 is regarded to be a potential promotor of tumor growth. However, the involvement of CD164 in human glioma proliferation and apoptosis remains unknown. The aim of the present study was to investigate the expression and oncogenic function of CD164 in normal human astrocytes (NHA) and glioma cells in vitro and in vivo. The results of the present study demonstrated that CD164 mRNA and protein levels were significantly increased in human glioma cell lines and tissue samples. CD164 overexpression promoted the proliferation of NHA in vitro, and its tumorigenic effect was confirmed in a murine xenograft model. Knockdown of CD164 inhibited cell proliferation and promoted apoptosis of the U87 human glioma cell line in vitro and in vivo. In addition, knockdown of CD164 was demonstrated to upregulate the Bax/Bcl2 ratio and phosphatase and tensin homolog (PTEN) expression, reduce protein kinase B (AKT) phosphorylation and promote the expression of p53 in U87 cells. The results suggest that CD164 expression may have affected the proliferation and apoptosis of human glioma cells via the PTEN/phosphoinositide 3-kinase/AKT pathway, and may therefore present a potential target for the diagnosis and treatment of glioma.
[Mh] Termos MeSH primário: Apoptose
Neoplasias Encefálicas/enzimologia
Neoplasias Encefálicas/patologia
Glioma/enzimologia
Glioma/patologia
PTEN Fosfo-Hidrolase/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Neoplasias Encefálicas/genética
Linhagem Celular Tumoral
Proliferação Celular
Regulação para Baixo/genética
Endolina/genética
Endolina/metabolismo
Regulação Neoplásica da Expressão Gênica
Técnicas de Silenciamento de Genes
Inativação Gênica
Glioma/genética
Células HEK293
Seres Humanos
Imuno-Histoquímica
Camundongos Endogâmicos BALB C
Camundongos Nus
Fosfatidilinositol 3-Quinases/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/metabolismo
Transdução de Sinais
Regulação para Cima/genética
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD164 protein, human); 0 (Endolyn); 0 (RNA, Messenger); 0 (RNA, Small Interfering); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 3.1.3.67 (PTEN Phosphohydrolase); EC 3.1.3.67 (PTEN protein, human)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2017.6204


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[PMID]:27766406
[Au] Autor:Benoit BM; Jariwala N; O'Connor G; Oetjen LK; Whelan TM; Werth A; Troxel AB; Sicard H; Zhu L; Miller C; Takeshita J; McVicar DW; Kim BS; Rook AH; Wysocka M
[Ad] Endereço:Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, 421 Curie Blvd, 1049 BRB, Philadelphia, PA, 19104, USA.
[Ti] Título:CD164 identifies CD4 T cells highly expressing genes associated with malignancy in Sézary syndrome: the Sézary signature genes, FCRL3, Tox, and miR-214.
[So] Source:Arch Dermatol Res;309(1):11-19, 2017 Jan.
[Is] ISSN:1432-069X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Sézary syndrome (SS), a leukemic variant of cutaneous T-cell lymphoma (CTCL), is associated with a significantly shorter life expectancy compared to skin-restricted mycosis fungoides. Early diagnosis of SS is, therefore, key to achieving enhanced therapeutic responses. However, the lack of a biomarker(s) highly specific for malignant CD4 T cells in SS patients has been a serious obstacle in making an early diagnosis. We recently demonstrated the high expression of CD164 on CD4 T cells from Sézary syndrome patients with a wide range of circulating tumor burdens. To further characterize CD164 as a potential biomarker for malignant CD4 T cells, CD164 and CD164 CD4 T cells isolated from patients with high-circulating tumor burden, B2 stage, and medium/low tumor burden, B1-B0 stage, were assessed for the expression of genes reported to differentiate SS from normal controls, and associated with malignancy and poor prognosis. The expression of Sézary signature genes: T plastin, GATA-3, along with FCRL3, Tox, and miR-214, was significantly higher, whereas STAT-4 was lower, in CD164 compared with CD164 CD4 T cells. While Tox was highly expressed in both B2 and B1-B0 patients, the expression of Sézary signature genes, FCRL3, and miR-214 was associated predominantly with advanced B2 disease. High expression of CD164 mRNA and protein was also detected in skin from CTCL patients. CD164 was co-expressed with KIR3DL2 on circulating CD4 T cells from high tumor burden SS patients, further providing strong support for CD164 as a disease relevant surface biomarker.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/genética
Linfócitos T CD4-Positivos/química
Proteínas de Grupo de Alta Mobilidade/genética
Linfócitos do Interstício Tumoral/química
MicroRNAs/genética
Receptores Imunológicos/genética
Síndrome de Sézary/genética
Neoplasias Cutâneas/genética
[Mh] Termos MeSH secundário: Biomarcadores Tumorais/análise
Linfócitos T CD4-Positivos/imunologia
Estudos de Casos e Controles
Endolina/análise
Endolina/genética
Citometria de Fluxo
Perfilação da Expressão Gênica/métodos
Regulação Neoplásica da Expressão Gênica
Proteínas de Grupo de Alta Mobilidade/análise
Seres Humanos
Linfócitos do Interstício Tumoral/imunologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Receptores Imunológicos/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Síndrome de Sézary/diagnóstico
Síndrome de Sézary/imunologia
Síndrome de Sézary/metabolismo
Neoplasias Cutâneas/diagnóstico
Neoplasias Cutâneas/imunologia
Neoplasias Cutâneas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (CD164 protein, human); 0 (Endolyn); 0 (FCRL3 protein, human); 0 (High Mobility Group Proteins); 0 (MIRN214 microRNA, human); 0 (MicroRNAs); 0 (RNA, Messenger); 0 (Receptors, Immunologic); 0 (TOX protein, human)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161022
[St] Status:MEDLINE
[do] DOI:10.1007/s00403-016-1698-8


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[PMID]:26524186
[Au] Autor:Guenova E; Ignatova D; Chang YT; Contassot E; Mehra T; Saulite I; Navarini AA; Mitev V; Dummer R; Kazakov DV; French LE; Hoetzenecker W; Cozzio A
[Ad] Endereço:Department of Dermatology, University Hospital of Zürich, Gloriastr. 31, 8091 Zürich, Switzerland. Emmanuella.Guenova@usz.ch.
[Ti] Título:Expression of CD164 on Malignant T cells in Sézary Syndrome.
[So] Source:Acta Derm Venereol;96(4):464-7, 2016 May.
[Is] ISSN:1651-2057
[Cp] País de publicação:Sweden
[La] Idioma:eng
[Ab] Resumo:Sézary syndrome is a primary cutaneous T-cell lymphoma characterized by pruritic erythroderma, peripheral lymphadenopathy and the presence of malignant T cells in the blood. Unequivocal detection of malignant cells in patients with Sézary syndrome is of important diagnostic, prognostic and therapeutic value. However, no single Sézary syndrome specific cell surface marker has been identified. In a cohort of patients with Sézary syndrome, CD164 expression on total CD4+ lymphocytes was significantly upregulated compared with healthy controls. CD164 expression was in most cases limited to CD4+CD26- malignant T lymphocytes, unequivocally identified using flow-cytometry by the expression of a specific Vß clone for each patient. Increased expression of CD164 may be a promising diagnostic parameter and a potential target for a CD164-linked therapeutic approach in Sézary syndrome.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/sangue
Linfócitos T CD4-Positivos/imunologia
Síndrome de Sézary/sangue
Neoplasias Cutâneas/sangue
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Estudos de Casos e Controles
Células Cultivadas
Dipeptidil Peptidase 4/sangue
Endolina/sangue
Feminino
Citometria de Fluxo
Seres Humanos
Imunofenotipagem/métodos
Masculino
Meia-Idade
Fenótipo
Síndrome de Sézary/diagnóstico
Síndrome de Sézary/imunologia
Síndrome de Sézary/terapia
Neoplasias Cutâneas/diagnóstico
Neoplasias Cutâneas/imunologia
Neoplasias Cutâneas/terapia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (CD164 protein, human); 0 (Endolyn); EC 3.4.14.5 (DPP4 protein, human); EC 3.4.14.5 (Dipeptidyl Peptidase 4)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151103
[St] Status:MEDLINE
[do] DOI:10.2340/00015555-2264


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[PMID]:26197441
[Au] Autor:Nyegaard M; Rendtorff ND; Nielsen MS; Corydon TJ; Demontis D; Starnawska A; Hedemand A; Buniello A; Niola F; Overgaard MT; Leal SM; Ahmad W; Wikman FP; Petersen KB; Crüger DG; Oostrik J; Kremer H; Tommerup N; Frödin M; Steel KP; Tranebjærg L; Børglum AD
[Ad] Endereço:Department of Biomedicine, Aarhus University, Aarhus, Denmark; Centre for Integrative Sequencing (iSEQ), Aarhus University, Aarhus, Denmark.
[Ti] Título:A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
[So] Source:PLoS Genet;11(7):e1005386, 2015 Jul.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nonsyndromic hearing impairment (NSHI) is a highly heterogeneous condition with more than eighty known causative genes. However, in the clinical setting, a large number of NSHI families have unexplained etiology, suggesting that there are many more genes to be identified. In this study we used SNP-based linkage analysis and follow up microsatellite markers to identify a novel locus (DFNA66) on chromosome 6q15-21 (LOD 5.1) in a large Danish family with dominantly inherited NSHI. By locus specific capture and next-generation sequencing, we identified a c.574C>T heterozygous nonsense mutation (p.R192*) in CD164. This gene encodes a 197 amino acid transmembrane sialomucin (known as endolyn, MUC-24 or CD164), which is widely expressed and involved in cell adhesion and migration. The mutation segregated with the phenotype and was absent in 1200 Danish control individuals and in databases with whole-genome and exome sequence data. The predicted effect of the mutation was a truncation of the last six C-terminal residues of the cytoplasmic tail of CD164, including a highly conserved canonical sorting motif (YXXФ). In whole blood from an affected individual, we found by RT-PCR both the wild-type and the mutated transcript suggesting that the mutant transcript escapes nonsense mediated decay. Functional studies in HEK cells demonstrated that the truncated protein was almost completely retained on the plasma cell membrane in contrast to the wild-type protein, which targeted primarily to the endo-lysosomal compartments, implicating failed endocytosis as a possible disease mechanism. In the mouse ear, we found CD164 expressed in the inner and outer hair cells of the organ of Corti, as well as in other locations in the cochlear duct. In conclusion, we have identified a new DFNA locus located on chromosome 6q15-21 and implicated CD164 as a novel gene for hearing impairment.
[Mh] Termos MeSH primário: Endolina/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Linhagem Celular
Códon sem Sentido/genética
Surdez/genética
Dinamarca
Família
Feminino
Células HEK293
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Repetições de Microssatélites/genética
Órgão Espiral/metabolismo
Linhagem
Polimorfismo de Nucleotídeo Único/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Codon, Nonsense); 0 (Endolyn)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150722
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1005386


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[PMID]:25541484
[Au] Autor:Corvan SM; Agnew L; Andronicos NM
[Ad] Endereço:CSIRO Animal Health and Food Science, F.D McMaster Laboratory, Armidale NSW 2350, Australia; School of Science and Technology, University of New England, Armidale NSW 2350, Australia. Electronic address: Sinead.Corvan@csiro.au.
[Ti] Título:Trichostrongylus colubriformis induces IgE-independent CD13, CD164 and CD203c mediated activation of basophils in an in vitro intestinal epithelial cell co-culture model.
[So] Source:Vet Parasitol;207(3-4):285-96, 2015 Jan 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Gastrointestinal nematodes pose a major risk to the farming of small ruminants worldwide. Infections are typically controlled by anthelmintics, however as resistance to anthelmintics increases, it is necessary that the mechanism of host responses are understood in order to develop alternative control options. It is hypothesised that basophils are involved in the initiation of an anti-parasite immune response, independent of IgE. In this study, the in vitro activation states of CD203c(+) basophil-like KU812 cells were determined in the presence of Trichostrongylus colubriformis parasitised HT29 epithelial cells with or without mucin. Cell surface expression of CD164, CD107a and CD13 antigens on gated CD203(+) cells were determined and qRT-PCR was used to examine gene expression changes of IL33 (a Th2 cytokine) and the high affinity IgE receptor (FcÉ›RIα) within the co-culture. When KU812 basophils encountered T. colubriformis and/or mucin in a parasitised epithelium, the basophils increased cell surface expression of CD13 and CD164 antigens, independent of IgE. T. colubriformis also increased the number of CD203c(+) KU812 cells that expressed CD13 and CD164 antigens. These data support the in vivo observations of T. colubriformis primary infections in guinea pigs and sheep.
[Mh] Termos MeSH primário: Antígenos CD/genética
Basófilos/imunologia
Células Epiteliais/parasitologia
Regulação da Expressão Gênica/imunologia
Intestinos/imunologia
Tricostrongilose/imunologia
Trichostrongylus/imunologia
[Mh] Termos MeSH secundário: Animais
Antígenos CD/imunologia
Antígenos CD13/genética
Linhagem Celular
Técnicas de Cocultura
Endolina/genética
Células Epiteliais/imunologia
Células HT29
Seres Humanos
Imunoglobulina E/imunologia
Larva/imunologia
Proteína 1 de Membrana Associada ao Lisossomo/genética
Atividade Motora/efeitos dos fármacos
Atividade Motora/imunologia
Mucinas/farmacologia
Diester Fosfórico Hidrolases/genética
Pirofosfatases/genética
Trichostrongylus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (ENPP3 protein, human); 0 (Endolyn); 0 (Lysosomal-Associated Membrane Protein 1); 0 (Mucins); 37341-29-0 (Immunoglobulin E); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.4.11.2 (CD13 Antigens); EC 3.6.1.- (Pyrophosphatases)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141227
[St] Status:MEDLINE


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[PMID]:24756834
[Au] Autor:Shi JA; Lu DL; Huang X; Tan W
[Ad] Endereço:Department of Psychiatry, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
[Ti] Título:miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164.
[So] Source:Int J Mol Med;34(1):237-43, 2014 Jul.
[Is] ISSN:1791-244X
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:It is known that microRNA-219 (miR-219) expression is downregulated in medulloblastoma. In the present study, we investigated the expression, targets and functional effects of miR-219 in D283-MED medulloblastoma cells. We first demonstrated that miR-219 not only inhibits proliferation, but also suppresses the invasion and migration of D283-MED cells. Moreover, the knockdown of miR-219 promoted the proliferation, migration and invasion of the D283-MED cells. Secondly, we predicted that miR-219 targets the 3' untranslated region (3'UTR) of CD164 and orthodenticle homeobox 2 (OTX2) and then confirmed that it significantly downregulated the protein expression of CD164 and OTX2 in D283-MED cells. Finally, we demonstrated that the proliferation, invasion and migration of D283-MED cells were promoted by theectopic expression of CD164. These results indicate that miR-219 suppresses the proliferation, migration and invasion of medulloblastoma cells by targeting CD164. The results also suggest that miR-219 may serve as a potential therapeutic agent for medulloblastoma.
[Mh] Termos MeSH primário: Regulação Neoplásica da Expressão Gênica
Meduloblastoma/genética
MicroRNAs/genética
Fatores de Transcrição Otx/genética
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
Sequência de Bases
Linhagem Celular Tumoral
Movimento Celular
Proliferação Celular
Endolina/genética
Endolina/metabolismo
Seres Humanos
Meduloblastoma/metabolismo
Meduloblastoma/patologia
MicroRNAs/antagonistas & inibidores
MicroRNAs/metabolismo
Dados de Sequência Molecular
Fatores de Transcrição Otx/metabolismo
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (CD164 protein, human); 0 (Endolyn); 0 (MIRN219 microRNA, human); 0 (MicroRNAs); 0 (OTX2 protein, human); 0 (Otx Transcription Factors); 0 (RNA, Small Interfering)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140424
[St] Status:MEDLINE
[do] DOI:10.3892/ijmm.2014.1749


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[PMID]:23792457
[Au] Autor:Wysocka M; Kossenkov AV; Benoit BM; Troxel AB; Singer E; Schaffer A; Kim B; Dentchev T; Nagata S; Ise T; Showe LC; Rook AH
[Ad] Endereço:Department of Dermatology, University of Pennsylvania, Philadelphia, Pennsylvania, USA. Electronic address: mwysocka@mail.med.upenn.edu.
[Ti] Título:CD164 and FCRL3 are highly expressed on CD4+CD26- T cells in Sézary syndrome patients.
[So] Source:J Invest Dermatol;134(1):229-236, 2014 Jan.
[Is] ISSN:1523-1747
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sézary syndrome (SS) cells express cell surface molecules also found on normal activated CD4 T cells. In an effort to find a more specific surface marker for malignant SS cells, a microarray analysis of gene expression was performed. Results showed significantly increased levels of mRNA for CD164, a sialomucin found on human CD34+ hematopoietic stem cells, and FCRL3, a molecule present on a subset of human natural T regulatory cells. Both markers were increased in CD4 T cells from SS patients compared with healthy donors (HD). Flow cytometry studies confirmed the increased expression of CD164 and FCRL3 primarily on CD4+CD26- T cells of SS patients. Importantly, a statistically significant correlation was found between an elevated percentage of CD4+CD164+ T cells and an elevated percentage of CD4+CD26- T cells in all tested SS patients but not in patients with mycosis fungoides and atopic dermatitis or HD. FCRL3 expression was significantly increased only in patients with high tumor burden. CD4+CD164+ cells displayed cerebriform morphology and their loss correlated with clinical improvement in treated patients. Our results suggest that CD164 can serve as a marker for diagnosis and for monitoring progression of cutaneous T-cell lymphoma (CTCL)/SS and that FCRL3 expression correlates with a high circulating tumor burden.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Endolina/imunologia
Células Neoplásicas Circulantes/imunologia
Receptores Imunológicos/imunologia
Síndrome de Sézary/imunologia
[Mh] Termos MeSH secundário: Biomarcadores/metabolismo
Linfócitos T CD4-Positivos/metabolismo
Forma Celular/imunologia
Dipeptidil Peptidase 4/genética
Dipeptidil Peptidase 4/imunologia
Dipeptidil Peptidase 4/metabolismo
Progressão da Doença
Endolina/genética
Endolina/metabolismo
Citometria de Fluxo
Seres Humanos
Glicoproteínas de Membrana/genética
Proteínas dos Microfilamentos/genética
Células Neoplásicas Circulantes/metabolismo
Receptores Imunológicos/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Endolyn); 0 (FCRL3 protein, human); 0 (Membrane Glycoproteins); 0 (Microfilament Proteins); 0 (Receptors, Immunologic); 0 (plastin); EC 3.4.14.5 (DPP4 protein, human); EC 3.4.14.5 (Dipeptidyl Peptidase 4)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130625
[St] Status:MEDLINE


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[PMID]:24094005
[Au] Autor:Huang AF; Chen MW; Huang SM; Kao CL; Lai HC; Chan JY
[Ad] Endereço:Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan, Republic of China. jchan9473@gmail.com.
[Ti] Título:CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1α/CXCR4 axis.
[So] Source:Mol Cancer;12(1):115, 2013 Oct 05.
[Is] ISSN:1476-4598
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: CD164 (endolyn), a sialomucin, has been reported to play a role in the proliferation, adhesion, and differentiation of hematopoietic stem cells. The potential association of CD164 with tumorigenicity remains unclear. METHODS: The clinicopathological correlation of ovarian cancer with CD164 was assessed in a 97-patient tumor tissue microarray. Overexpression or silence CD164 was to analyze the effect of CD164 on the proliferation, colony formation and apoptosis via a mouse xenograft and western blotting analysis. The subcellular localization of CD164 was collected in the immunohistochemical and confocal analysis. RESULTS: Our data demonstrated that higher expression levels of CD164 were identified in malignant ovarian cancer cell lines, such as SKOV3 and HeyA8. The clinicopathological correlation analysis showed that the upregulation of CD164 protein was significantly associated with tumor grade and metastasis. The overexpression of CD164 in human ovarian epithelial surface cells promoted cellular proliferation and colony formation and suppressed apoptosis. These tumorigenicity effects of CD164 were reconfirmed in a mouse xenograft model. We also found that the overexpression of CD164 proteins increased the amounts of CXCR4 and SDF-1α and activated the SDF-1α/CXCR4 axis, inducing colony and sphere formation. Finally, we identified the subcellular localization of CD164 in the nucleus and cytosol and found that nuclear CD164 might be involved in the regulation of the activity of the CXCR4 promoter. CONCLUSIONS: Our findings suggest that the increased expression of CD164 is involved in ovarian cancer progression via the SDF-1α/CXCR4 axis, which promotes tumorigenicity. Thus, targeting CD164 may serve as a potential ovarian cancer biomarker, and targeting CD164 may serve as a therapeutic modality in the management of high-grade ovarian tumors.
[Mh] Termos MeSH primário: Quimiocina CXCL12/metabolismo
Neoplasias Epiteliais e Glandulares/metabolismo
Neoplasias Ovarianas/metabolismo
Receptores CXCR4/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores Tumorais/metabolismo
Linhagem Celular Tumoral
Transformação Celular Neoplásica/metabolismo
Endolina/fisiologia
Células Epiteliais/metabolismo
Células Epiteliais/patologia
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Camundongos
Camundongos Nus
Meia-Idade
Transplante de Neoplasias
Neoplasias Epiteliais e Glandulares/patologia
Células-Tronco Neoplásicas/metabolismo
Neoplasias Ovarianas/patologia
Fosfatidilinositol 3-Quinases/metabolismo
Prognóstico
Regiões Promotoras Genéticas
Proteínas Proto-Oncogênicas c-akt/metabolismo
Receptores CXCR4/genética
Análise Serial de Tecidos
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Ativação Transcricional
Carga Tumoral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (CD164 protein, human); 0 (CXCR4 protein, human); 0 (Chemokine CXCL12); 0 (Endolyn); 0 (Receptors, CXCR4); 0 (Transcription Factors); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131008
[St] Status:MEDLINE
[do] DOI:10.1186/1476-4598-12-115


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[PMID]:22976307
[Au] Autor:Mo D; Ihrke G; Costa SA; Brilli L; Labilloy A; Halfter W; Cianciolo Cosentino C; Hukriede NA; Weisz OA
[Ad] Endereço:Renal Electrolyte Division, University of Pittsburgh School of Medicine Pittsburgh, PA 15261 USA.
[Ti] Título:Apical targeting and endocytosis of the sialomucin endolyn are essential for establishment of zebrafish pronephric kidney function.
[So] Source:J Cell Sci;125(Pt 22):5546-54, 2012 Nov 15.
[Is] ISSN:1477-9137
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Kidney function requires the appropriate distribution of membrane proteins between the apical and basolateral surfaces along the kidney tubule. Further, the absolute amount of a protein at the cell surface versus intracellular compartments must be attuned to specific physiological needs. Endolyn (CD164) is a transmembrane protein that is expressed at the brush border and in apical endosomes of the proximal convoluted tubule and in lysosomes of more distal segments of the kidney. Endolyn has been shown to regulate CXCR4 signaling in hematopoietic precursor cells and myoblasts; however, little is known about endolyn function in the adult or developing kidney. Here we identify endolyn as a gene important for zebrafish pronephric kidney function. Zebrafish endolyn lacks the N-terminal mucin-like domain of the mammalian protein, but is otherwise highly conserved. Using in situ hybridization we show that endolyn is expressed early during development in zebrafish brain, eye, gut and pronephric kidney. Embryos injected with a translation-inhibiting morpholino oligonucleotide targeted against endolyn developed pericardial edema, hydrocephaly and body curvature. The pronephric kidney appeared normal morphologically, but clearance of fluorescent dextran injected into the common cardinal vein was delayed, consistent with a defect in the regulation of water balance in morphant embryos. Heterologous expression of rat endolyn rescued the morphant phenotypes. Interestingly, rescue experiments using mutant rat endolyn constructs revealed that both apical sorting and endocytic/lysosomal targeting motifs are required for normal pronephric kidney function. This suggests that both polarized targeting and postendocytic trafficking of endolyn are essential for the protein's proper function in mammalian kidney.
[Mh] Termos MeSH primário: Polaridade Celular
Endocitose
Endolina/metabolismo
Rim/embriologia
Rim/metabolismo
Pronefro/embriologia
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Envelhecimento/metabolismo
Animais
Polaridade Celular/efeitos dos fármacos
Embrião não Mamífero/efeitos dos fármacos
Embrião não Mamífero/metabolismo
Endocitose/efeitos dos fármacos
Endolina/química
Técnicas de Silenciamento de Genes
Rim/anatomia & histologia
Rim/citologia
Células Madin Darby de Rim Canino
Mamíferos/embriologia
Mamíferos/metabolismo
Morfolinos/farmacologia
Especificidade de Órgãos
Pronefro/metabolismo
Estrutura Terciária de Proteína
Ratos
Relação Estrutura-Atividade
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Endolyn); 0 (Morpholinos)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120915
[St] Status:MEDLINE
[do] DOI:10.1242/jcs.111468


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[PMID]:22855528
[Au] Autor:Mo D; Costa SA; Ihrke G; Youker RT; Pastor-Soler N; Hughey RP; Weisz OA
[Ad] Endereço:Renal Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
[Ti] Título:Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism.
[So] Source:Mol Biol Cell;23(18):3636-46, 2012 Sep.
[Is] ISSN:1939-4586
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sialomucin endolyn is implicated in adhesion, migration, and differentiation of various cell types. Along rat kidney tubules, endolyn is variously localized to the apical surface and endosomal/lysosomal compartments. Apical delivery of newly synthesized rat endolyn predominates over direct lysosomal delivery in polarized Madin-Darby canine kidney cells. Apical sorting depends on terminal processing of a subset of lumenal N-glycans. Here we dissect the requirements of N-glycan processing for apical targeting and investigate the underlying mechanism. Modulation of glycan branching and subsequent polylactosamine elongation by knockdown of N-acetylglucosaminyltransferase III or V had no effect on apical delivery of endolyn. In contrast, combined but not individual knockdown of sialyltransferases ST3Gal-III, ST3Gal-IV, and ST6Gal-I, which together are responsible for addition of α2,3- and α2,6-linked sialic acids on N-glycans, dramatically decreased endolyn surface polarity. Endolyn synthesized in the presence of kifunensine, which blocks terminal N-glycan processing, reduced its interaction with several recombinant canine galectins, and knockdown of galectin-9 (but not galectin-3, -4, or -8) selectively disrupted endolyn polarity. Our data suggest that sialylation enables recognition of endolyn by galectin-9 to mediate efficient apical sorting. They raise the intriguing possibility that changes in glycosyltransferase expression patterns and/or galectin-9 distribution may acutely modulate endolyn trafficking in the kidney.
[Mh] Termos MeSH primário: Endolina/metabolismo
Galectinas/metabolismo
Rim/metabolismo
Polissacarídeos/metabolismo
[Mh] Termos MeSH secundário: Alcaloides/farmacologia
Animais
Cães
Eletroforese em Gel de Poliacrilamida
Endolina/genética
Galectinas/genética
Expressão Gênica
Rim/citologia
Rim/efeitos dos fármacos
Túbulos Renais/metabolismo
Lisossomos/metabolismo
Células Madin Darby de Rim Canino
Microscopia Confocal
N-Acetilglucosaminiltransferases/genética
N-Acetilglucosaminiltransferases/metabolismo
Interferência de RNA
Ratos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Ácidos Siálicos/metabolismo
Sialiltransferases/genética
Sialiltransferases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Endolyn); 0 (Galectins); 0 (Polysaccharides); 0 (Sialic Acids); 0 (galectin 9, rat); 0NI8960271 (kifunensine); EC 2.4.1.- (N-Acetylglucosaminyltransferases); EC 2.4.1.144 (beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase); EC 2.4.1.155 (alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase); EC 2.4.99.- (Sialyltransferases); EC 2.4.99.4 (beta-galactoside alpha-2,3-sialyltransferase)
[Em] Mês de entrada:1303
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120803
[St] Status:MEDLINE
[do] DOI:10.1091/mbc.E12-04-0329



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