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  1 / 999 MEDLINE  
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[PMID]:28791819
[Au] Autor:Kosacka M; Porebska I; Jankowska R
[Ad] Endereço:Department of Pulmonology and Lung Cancer, Wroclaw Medical University, Poland.
[Ti] Título:Decreased sL-selectin serum levels in sleep apnea syndrome patients with cardiovascular diseases.
[So] Source:Adv Clin Exp Med;26(3):449-453, 2017 May-Jun.
[Is] ISSN:1899-5276
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Obstructive sleep apnea syndrome (OSA) is a common disorder associated with an increased risk of cardiovascular diseases. OBJECTIVES: sL-selectin is an adhesion molecule released from the surface of leukocytes as they are activated and may inhibit leukocyte attachment to the endothelium. The aim of this study was to evaluate sL-selectin serum levels in OSA patients with cardiovascular diseases. MATERIAL AND METHODS: A total of 163 OSA patients were enrolled in the study. The mean age was 55.41 ± 8.63 years and the mean AHI (apnea hypopnea index) was 35.02 ± 22.28/h. A control group was composed of 59 healthy subjects. All subjects underwent a nocturnal respiratory polygraphy. sL-selectin serum levels were measured using the enzyme-linked immunosorbent assay (ELISA) method. RESULTS: sL-selectin serum levels were significantly lower in OSA patients than in the control group (1080.02 ± 175.29 vs 1350.73 ± 569.75 ng/mL, p < 0.05). In addition, there was a negative correlation between sL-selectin levels and AHI and DI and a positive correlation between sL-selectin levels and mean and minimum saturation. sL-selectin levels were lower in OSA patients with cardiovascular diseases than in those without co-morbidities. We also found that sL-selectin correlated positively with HDL-cholesterol (high density lipoprotein) and negatively with uric acid and CRP (C-reactive protein). CONCLUSIONS: Our work, together with observations relating to other diseases and experimental studies, suggests that lower sL-selectin levels could play a role in an increased risk of cardiovascular complications in sleep apnea syndrome. However future studies are needed to understand the role of sL-selectin in sleep apnea syndrome.
[Mh] Termos MeSH primário: Selectinas/sangue
Síndromes da Apneia do Sono/sangue
Apneia Obstrutiva do Sono/sangue
[Mh] Termos MeSH secundário: Biomarcadores/sangue
Proteína C-Reativa/metabolismo
Doenças Cardiovasculares/sangue
Doenças Cardiovasculares/metabolismo
HDL-Colesterol/sangue
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Seres Humanos
Masculino
Meia-Idade
Polissonografia/métodos
Fatores de Risco
Síndromes da Apneia do Sono/metabolismo
Apneia Obstrutiva do Sono/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cholesterol, HDL); 0 (Selectins); 9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.17219/acem/62215


  2 / 999 MEDLINE  
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[PMID]:28628637
[Au] Autor:Veach RA; Liu Y; Zienkiewicz J; Wylezinski LS; Boyd KL; Wynn JL; Hawiger J
[Ad] Endereço:Immunotherapy Program at Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America.
[Ti] Título:Survival, bacterial clearance and thrombocytopenia are improved in polymicrobial sepsis by targeting nuclear transport shuttles.
[So] Source:PLoS One;12(6):e0179468, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The rising tide of sepsis, a leading cause of death in the US and globally, is not adequately controlled by current antimicrobial therapies and supportive measures, thereby requiring new adjunctive treatments. Severe microvascular injury and multiple organ failure in sepsis are attributed to a "genomic storm" resulting from changes in microbial and host genomes encoding virulence factors and endogenous inflammatory mediators, respectively. This storm is mediated by stress-responsive transcription factors that are ferried to the nucleus by nuclear transport shuttles importins/karyopherins. We studied the impact of simultaneously targeting two of these shuttles, importin alpha 5 (Imp α5) and importin beta 1 (Imp ß1), with a cell-penetrating Nuclear Transport Modifier (NTM) in a mouse model of polymicrobial sepsis. NTM reduced nuclear import of stress-responsive transcription factors nuclear factor kappa B, signal transducer and activator of transcription 1 alpha, and activator protein 1 in liver, which was also protected from sepsis-associated metabolic changes. Strikingly, NTM without antimicrobial therapy improved bacterial clearance in blood, spleen, and lungs, wherein a 700-fold reduction in bacterial burden was achieved while production of proinflammatory cytokines and chemokines in blood plasma was suppressed. Furthermore, NTM significantly improved thrombocytopenia, a prominent sign of microvascular injury in sepsis, inhibited neutrophil infiltration in the liver, decreased L-selectin, and normalized plasma levels of E-selectin and P-selectin, indicating reduced microvascular injury. Importantly, NTM combined with antimicrobial therapy extended the median time to death from 42 to 83 hours and increased survival from 30% to 55% (p = 0.022) as compared to antimicrobial therapy alone. This study documents the fundamental role of nuclear signaling mediated by Imp α5 and Imp ß1 in the mechanism of polymicrobial sepsis and highlights the potential for targeting nuclear transport as an adjunctive therapy in sepsis management.
[Mh] Termos MeSH primário: Transporte Ativo do Núcleo Celular/efeitos dos fármacos
Peptídeos Penetradores de Células/farmacologia
Proteínas Nucleares/metabolismo
Sepse/patologia
alfa Carioferinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/uso terapêutico
Peptídeos Penetradores de Células/síntese química
Peptídeos Penetradores de Células/química
Quimiocinas/sangue
Citocinas/sangue
Modelos Animais de Doenças
Feminino
Fígado/imunologia
Fígado/metabolismo
Fígado/patologia
Camundongos
Camundongos Endogâmicos C57BL
Neutrófilos/citologia
Neutrófilos/imunologia
Proteínas Nucleares/antagonistas & inibidores
Selectinas/sangue
Sepse/tratamento farmacológico
Sepse/microbiologia
Sepse/mortalidade
Taxa de Sobrevida
Trombocitopenia/patologia
Fator de Transcrição RelA/metabolismo
alfa Carioferinas/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Cell-Penetrating Peptides); 0 (Chemokines); 0 (Cytokines); 0 (KPNA1 protein, mouse); 0 (Kpnb1 protein, mouse); 0 (Nuclear Proteins); 0 (Selectins); 0 (Transcription Factor RelA); 0 (alpha Karyopherins); 0 (cSN50.1 peptide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179468


  3 / 999 MEDLINE  
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[PMID]:28419109
[Au] Autor:Chen X; Zhang S; Cheng Z; Cooke JS; Werling D; Wathes DC; Pollott GE
[Ad] Endereço:Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China.
[Ti] Título:Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows.
[So] Source:PLoS One;12(4):e0175555, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Selectins are adhesion molecules, which mediate attachment between leucocytes and endothelium. They aid extravasation of leucocytes from blood into inflamed tissue during the mammary gland's response to infection. Selectins are also involved in attachment of the conceptus to the endometrium and subsequent placental development. Poor fertility and udder health are major causes for culling dairy cows. The three identified bovine selectin genes SELP, SELL and SELE are located in a gene cluster. SELP is the most polymorphic of these genes. Several SNP in SELP and SELE are associated with human vascular disease, while SELP SNP rs6127 has been associated with recurrent pregnancy loss in women. This study describes the results of a gene association study for SNP in SELP (n = 5), SELL (n = 2) and SELE (n = 1) with fertility, milk production and longevity traits in a population of 337 Holstein Friesian dairy cows. Blood samples for PCR-RFLP were collected at 6 months of age and animals were monitored until either culling or 2,340 days from birth. Three SNP in SELPEx4-6 formed a haplotype block containing a Glu/Ala substitution at rs42312260. This region was associated with poor fertility and reduced survival times. SELPEx8 (rs378218397) coded for a Val475Met variant locus in the linking region between consensus repeats 4 and 5, which may influence glycosylation. The synonymous SNP rs110045112 in SELEEx14 deviated from Hardy Weinberg equilibrium. For both this SNP and rs378218397 there were too few AA homozygotes present in the population and AG heterozygotes had significantly worse fertility than GG homozygotes. Small changes in milk production associated with some SNP could not account for the reduced fertility and only SELPEx6 showed any association with somatic cell count. These results suggest that polymorphisms in SELP and SELE are associated with the likelihood of successful pregnancy, potentially through compromised implantation and placental development.
[Mh] Termos MeSH primário: Bovinos/genética
Fertilidade/genética
Família Multigênica/genética
Polimorfismo de Nucleotídeo Único
Selectinas/genética
[Mh] Termos MeSH secundário: Alelos
Animais
Éxons/genética
Feminino
Frequência do Gene
Genótipo
Desequilíbrio de Ligação
Leite/metabolismo
Fenótipo
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Isoformas de Proteínas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Isoforms); 0 (Selectins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175555


  4 / 999 MEDLINE  
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[PMID]:28196850
[Au] Autor:Wu F; Liu L; Zhou H
[Ad] Endereço:Department of Immunology, Bengbu Medical College, Bengbu, China.
[Ti] Título:Endothelial cell activation in central nervous system inflammation.
[So] Source:J Leukoc Biol;101(5):1119-1132, 2017 May.
[Is] ISSN:1938-3673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leukocyte migration across the endothelial barrier plays an essential role in CNS inflammation. The migration process requires complex endothelial adhesion molecules concentrated at the junctions of endothelial cells. Recent findings suggest that cerebral endothelial cells play an active role in the pathogenesis of CNS inflammatory diseases. This review describes our current understanding of the effects of various inflammatory mediators of leukocyte migration on cerebral endothelial cells, the mechanisms underlying the leukocyte-endothelial cell interactions, and the crosstalk between endothelial cells and glial cells or platelets. These emerging mechanisms may provide new therapeutic strategies for a variety of CNS inflammatory diseases.
[Mh] Termos MeSH primário: Encéfalo/imunologia
Células Endoteliais/imunologia
Endotélio Vascular/imunologia
Regulação da Expressão Gênica/imunologia
Inflamação/imunologia
Leucócitos/imunologia
[Mh] Termos MeSH secundário: Animais
Plaquetas/imunologia
Plaquetas/patologia
Barreira Hematoencefálica/imunologia
Barreira Hematoencefálica/patologia
Encéfalo/patologia
Comunicação Celular
Movimento Celular
Citocinas/genética
Citocinas/imunologia
Células Endoteliais/patologia
Endotélio Vascular/patologia
Seres Humanos
Inflamação/genética
Inflamação/patologia
Integrinas/genética
Integrinas/imunologia
Leucócitos/patologia
Neuroglia/imunologia
Neuroglia/patologia
Selectinas/genética
Selectinas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cytokines); 0 (Integrins); 0 (Selectins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1189/jlb.3RU0816-352RR


  5 / 999 MEDLINE  
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[PMID]:28178038
[Au] Autor:Abadier M; Ley K
[Ad] Endereço:Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA.
[Ti] Título:P-selectin glycoprotein ligand-1 in T cells.
[So] Source:Curr Opin Hematol;24(3):265-273, 2017 May.
[Is] ISSN:1531-7048
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE OF REVIEW: We review P-selectin glycoprotein ligand-1 (PSGL-1) as a selectin and chemokine-binding adhesion molecule. PSGL-1 is widely studied in neutrophils. Here, we focus on T cells, because PSGL-1 was recently described as a major immunomodulatory molecule during viral infection. PSGL-1 also plays a crucial role in T-cell homeostasis by binding to lymphoid chemokines, and can induce tolerance by enhancing the functions of regulatory T cells. RECENT FINDINGS: PSGL-1 was originally described as a leukocyte ligand for P-selectin, but it is actually a ligand for all selectins (P-, L- and E-selectin), binds chemokines, activates integrins and profoundly affects T-cell biology. It has been shown recently that PSGL-1 can modulate T cells during viral infection by acting as a negative regulator for T-cell functions. Absence of PSGL-1 promotes effector CD4 and CD8 T-cell differentiation and prevents T-cell exhaustion. Consistent with this, tumor growth was significantly reduced in PSGL-1-deficient mice because of an enhanced number of effector T cells together with reduced levels of inhibitory receptors that induce T-cell exhaustion. SUMMARY: PSGL-1 is the best-studied selectin ligand and has become a posterchild of versatility in leukocyte adhesion, inflammation and immunology. The direct involvement of PSGL-1 in T-cell biology suggests that it might be a drug target. Indeed, PSGL-1 has been tested in some clinical trials and recently, PSGL-1 blockers were proposed as a potential cotherapy in cancer immunotherapy.
[Mh] Termos MeSH primário: Glicoproteínas de Membrana/genética
Glicoproteínas de Membrana/metabolismo
Linfócitos T/imunologia
Linfócitos T/metabolismo
[Mh] Termos MeSH secundário: Animais
Quimiocinas/metabolismo
Citoesqueleto/metabolismo
Glicosilação
Seres Humanos
Tolerância Imunológica
Imunomodulação
Integrinas/metabolismo
Glicoproteínas de Membrana/química
Microdomínios da Membrana/metabolismo
Ligação Proteica
Transporte Proteico
Selectinas/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Chemokines); 0 (Integrins); 0 (Membrane Glycoproteins); 0 (P-selectin ligand protein); 0 (Selectins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1097/MOH.0000000000000331


  6 / 999 MEDLINE  
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[PMID]:28115423
[Au] Autor:Feng Y; Ma X; Deng L; Yao B; Xiong Y; Wu Y; Wang L; Ma Q; Ma F
[Ad] Endereço:Sichuan University - The Chinese University of Hong Kong Joint Laboratory for Reproductive Medicine, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. Ch
[Ti] Título:Role of selectins and their ligands in human implantation stage.
[So] Source:Glycobiology;27(5):385-391, 2017 May 01.
[Is] ISSN:1460-2423
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Selectins are a family of calcium-dependent, type I transmembrane, carbohydrate-binding glycoproteins. Selectins and their ligands are not only involved in physiological processes such as leukocyte homing and pathological processes such as cancer, but also play an essential role in the human implantation. L-selectin and its ligands participate in the adhesion of the blastocyst to the endometrium at the maternal-fetal interface. P-selectin and E-selectin are involved in immune recognition of maternal decidua to the embedded embryo as well as trophoblast migration within decidual spiral arterioles. Moreover, altered expression of selectins and their ligands are found to be associated with some abnormal pregnancies and infertilities. This review focuses on the current progress of research on the role of selectins and their ligands in the human implantation process.
[Mh] Termos MeSH primário: Implantação do Embrião/genética
Selectinas/metabolismo
Trofoblastos/metabolismo
[Mh] Termos MeSH secundário: Decídua/crescimento & desenvolvimento
Decídua/metabolismo
Endométrio/crescimento & desenvolvimento
Endométrio/metabolismo
Feminino
Seres Humanos
Ligantes
Masculino
Gravidez
Selectinas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Ligands); 0 (Selectins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.1093/glycob/cwx009


  7 / 999 MEDLINE  
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[PMID]:27975143
[Au] Autor:Bhide GP; Colley KJ
[Ad] Endereço:Department of Biochemistry and Molecular Genetics, College of Medicine, The University of Illinois at Chicago, 900 S. Ashland Avenue, MC669, Chicago, IL, 60607, USA.
[Ti] Título:Sialylation of N-glycans: mechanism, cellular compartmentalization and function.
[So] Source:Histochem Cell Biol;147(2):149-174, 2017 Feb.
[Is] ISSN:1432-119X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Sialylated N-glycans play essential roles in the immune system, pathogen recognition and cancer. This review approaches the sialylation of N-glycans from three perspectives. The first section focuses on the sialyltransferases that add sialic acid to N-glycans. Included in the discussion is a description of these enzymes' glycan acceptors, conserved domain organization and sequences, molecular structure and catalytic mechanism. In addition, we discuss the protein interactions underlying the polysialylation of a select group of adhesion and signaling molecules. In the second section, the biosynthesis of sialic acid, CMP-sialic acid and sialylated N-glycans is discussed, with a special emphasis on the compartmentalization of these processes in the mammalian cell. The sequences and mechanisms maintaining the sialyltransferases and other glycosylation enzymes in the Golgi are also reviewed. In the final section, we have chosen to discuss processes in which sialylated glycans, both N- and O-linked, play a role. The first part of this section focuses on sialic acid-binding proteins including viral hemagglutinins, Siglecs and selectins. In the second half of this section, we comment on the role of sialylated N-glycans in cancer, including the roles of ß1-integrin and Fas receptor N-glycan sialylation in cancer cell survival and drug resistance, and the role of these sialylated proteins and polysialic acid in cancer metastasis.
[Mh] Termos MeSH primário: Células/metabolismo
Polissacarídeos/metabolismo
Ácidos Siálicos/química
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Neoplasias/fisiopatologia
Polissacarídeos/química
Selectinas/química
Lectinas Semelhantes a Imunoglobulina de Ligação ao Ácido Siálico/química
Ácidos Siálicos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Polysaccharides); 0 (Selectins); 0 (Sialic Acid Binding Immunoglobulin-like Lectins); 0 (Sialic Acids); 0 (polysialic acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1007/s00418-016-1520-x


  8 / 999 MEDLINE  
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Registro de Ensaios Clínicos
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[PMID]:27881395
[Au] Autor:Joris PJ; Plat J; Kusters YH; Houben AJ; Stehouwer CD; Schalkwijk CG; Mensink RP
[Ad] Endereço:Departments of Human Biology, NUTRIM (School of Nutrition and Translational Research in Metabolism) and p.joris@maastrichtuniversity.nl.
[Ti] Título:Diet-induced weight loss improves not only cardiometabolic risk markers but also markers of vascular function: a randomized controlled trial in abdominally obese men.
[So] Source:Am J Clin Nutr;105(1):23-31, 2017 Jan.
[Is] ISSN:1938-3207
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Many trials assessing effects of dietary weight loss on vascular function have been performed without no-weight loss control groups and in individuals with obesity-related morbidities. Usually a limited set of vascular function markers has been investigated. OBJECTIVE: The objective of this study was to examine effects of diet-induced weight loss on various vascular function markers and differences between normal-weight and abdominally obese men at baseline and after weight reduction. DESIGN: Twenty-five healthy, normal-weight men (waist circumference: <94 cm) and 54 abdominally obese men (waist circumference: 102-110 cm) participated. Abdominally obese participants were randomly allocated to a dietary weight-loss or a no-weight loss control group. Individuals from the weight-loss group followed a calorie-restricted diet for 6 wk to obtain a waist circumference <102 cm followed by a weight-maintenance period of 2 wk. The control group maintained their habitual diet and physical activity levels. The primary outcome was the change in brachial artery flow-mediated vasodilation (FMD). RESULTS: Compared with the control group, FMD did not change in the weight-loss group, but carotid-to-femoral pulse wave velocity tended to decrease by 0.5 m/s (P = 0.065). The retinal arteriolar caliber increased by 5 µm (P < 0.001) and the arteriolar-to-venular ratio by 0.02 (P < 0.01). Soluble endothelial selectin and soluble intercellular adhesion molecule concentrations decreased (P < 0.001). Also, total cholesterol, low-density lipoprotein cholesterol, triacylglycerol, glucose, insulin, C-peptide, homeostasis model assessment of insulin resistance, and blood pressure improved (P < 0.05 for all variables). Except for FMD, these markers differed at baseline between normal-weight and abdominally obese men but became comparable after weight loss. CONCLUSIONS: In abdominally obese men, dietary weight loss targeting a waist circumference of <102 cm improved retinal microvascular caliber, plasma biomarkers of microvascular endothelial function, and the more conventional cardiometabolic risk markers. Aortic stiffness tended to decrease, but FMD was not changed. This trial was registered at clinicaltrials.gov as NCT01675401.
[Mh] Termos MeSH primário: Doenças Cardiovasculares/etiologia
Endotélio Vascular/fisiologia
Ingestão de Energia
Obesidade Abdominal/dietoterapia
Vasodilatação
Circunferência da Cintura
Perda de Peso/fisiologia
[Mh] Termos MeSH secundário: Adulto
Artérias/fisiologia
Biomarcadores/sangue
Glicemia/metabolismo
Pressão Sanguínea
Proteína C-Reativa/metabolismo
Doenças Cardiovasculares/sangue
Doenças Cardiovasculares/fisiopatologia
Doenças Cardiovasculares/prevenção & controle
Moléculas de Adesão Celular/sangue
Seres Humanos
Resistência à Insulina
Lipídeos/sangue
Masculino
Meia-Idade
Obesidade Abdominal/complicações
Obesidade Abdominal/fisiopatologia
Análise de Onda de Pulso
Fatores de Risco
Selectinas/sangue
Rigidez Vascular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Biomarkers); 0 (Blood Glucose); 0 (Cell Adhesion Molecules); 0 (Lipids); 0 (Selectins); 9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161125
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.3945/ajcn.116.143552


  9 / 999 MEDLINE  
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[PMID]:27566832
[Au] Autor:Buffone A; Nasirikenari M; Manhardt CT; Lugade A; Bogner PN; Sackstein R; Thanavala Y; Neelamegham S; Lau JT
[Ad] Endereço:Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York, USA.
[Ti] Título:Leukocyte-borne α(1,3)-fucose is a negative regulator of ß2-integrin-dependent recruitment in lung inflammation.
[So] Source:J Leukoc Biol;101(2):459-470, 2017 Feb.
[Is] ISSN:1938-3673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leukocyte recruitment in inflammation is a multistep, sequential cascade where the initial step is the selectin-dependent tethering, followed by the formation of firmer integrin-mediated adhesive forces leading to extravasation. The α(1,3)-fucose-containing sialyl-Lewis X (sLe ) is the archetypical ligand on leukocyte surfaces mediating selectin interactions. Canonically, disruption of α(1,3)-fucose formation ablates selectin-mediated adhesion, dramatically reducing trafficking. We report a paradoxical response to α(1,3)-fucose deficiency in which the loss exacerbated rather than attenuated leukocyte recruitment in a murine model of acute airway inflammation. The architecture of the capillary-dominated vasculature in the lung minimized the importance of the selectin dependent step, and we observed that α(1,3)-fucose deficiency augmented CXCR2-mediated Rap1-GTP signaling to enhance the ß -integrin-ICAM-1-binding axis. The data disclose a previously unknown function for α(1,3)-fucose, in which this structure negatively regulates the integrin activation step in leukocyte recruitment.
[Mh] Termos MeSH primário: Antígenos CD18/metabolismo
Fucose/metabolismo
Leucócitos/metabolismo
Pneumonia/metabolismo
Pneumonia/patologia
[Mh] Termos MeSH secundário: Animais
Adesão Celular
Comunicação Celular
Modelos Animais de Doenças
Fucosiltransferases/deficiência
Fucosiltransferases/metabolismo
Molécula 1 de Adesão Intercelular/metabolismo
Ligantes
Pulmão/patologia
Camundongos
Camundongos Endogâmicos C57BL
Modelos Biológicos
Ácido N-Acetilneuramínico/metabolismo
Neutrófilos/metabolismo
Neutrófilos/patologia
Peritônio/patologia
Receptores de Interleucina-8B/metabolismo
Selectinas/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD18 Antigens); 0 (Ligands); 0 (Receptors, Interleukin-8B); 0 (Selectins); 126547-89-5 (Intercellular Adhesion Molecule-1); 28RYY2IV3F (Fucose); EC 2.4.1.- (Fucosyltransferases); GZP2782OP0 (N-Acetylneuraminic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE
[do] DOI:10.1189/jlb.3A0516-215RR


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[PMID]:27623324
[Au] Autor:Cumba Garcia LM; Huseby Kelcher AM; Malo CS; Johnson AJ
[Ad] Endereço:Immunology Graduate Program, Mayo Clinic, Rochester, MN, United States.
[Ti] Título:Superior isolation of antigen-specific brain infiltrating T cells using manual homogenization technique.
[So] Source:J Immunol Methods;439:23-28, 2016 Dec.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Effective recovery of activated brain infiltrating lymphocytes is critical for investigations involving murine neurological disease models. To optimize lymphocyte recovery, we compared two isolation methods using brains harvested from seven-day Theiler's murine encephalomyelitis virus (TMEV) and TMEV-OVA infected mice. Brains were processed using either a manual dounce based approach or enzymatic digestion using type IV collagenase. The resulting cell suspensions from these two techniques were transferred to a percoll gradient, centrifuged, and lymphocytes were recovered. Flow cytometric analysis of CD45 cells showed greater percentage of CD44 CD62 activated lymphocytes and CD19+ B cells using the dounce method. In addition, we achieved a 3-fold greater recovery of activated virus-specific CD8 T cells specific for the immunodominant D :VP2 and engineered K :OVA epitopes through manual dounce homogenization approach as compared to collagenase digest. A greater percentage of viable cells was also achieved through dounce homogenization. Therefore, we conclude that manual homogenization is a superior approach to isolate activated T cells from the mouse brain.
[Mh] Termos MeSH primário: Antígenos/imunologia
Encéfalo/imunologia
Linfócitos T CD8-Positivos/imunologia
Infecções por Cardiovirus/imunologia
Separação Celular/métodos
Ativação Linfocitária
Ovalbumina/imunologia
Theilovirus/imunologia
[Mh] Termos MeSH secundário: Animais
Antígenos CD19/imunologia
Linfócitos B/imunologia
Encéfalo/metabolismo
Proteínas do Capsídeo/imunologia
Infecções por Cardiovirus/virologia
Sobrevivência Celular
Centrifugação com Gradiente de Concentração
Colágeno Tipo IV/metabolismo
Colagenases/metabolismo
Modelos Animais de Doenças
Epitopos
Feminino
Citometria de Fluxo
Receptores de Hialuronatos/imunologia
Masculino
Camundongos Endogâmicos C57BL
Povidona/química
Selectinas/imunologia
Dióxido de Silício/química
Theilovirus/patogenicidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Antigens, CD19); 0 (CD19 antigen, mouse); 0 (Capsid Proteins); 0 (Cd44 protein, mouse); 0 (Collagen Type IV); 0 (Epitopes); 0 (Hyaluronan Receptors); 0 (Selectins); 0 (VP2 protein, Theilovirus); 65455-52-9 (Percoll); 7631-86-9 (Silicon Dioxide); 9006-59-1 (Ovalbumin); EC 3.4.24.- (Collagenases); FZ989GH94E (Povidone)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE



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