| [PMID]: | 29216247 |
| [Au] Autor: | Brinkmann C; Hoffmann M; Lübke A; Nehlmeier I; Krämer-Kühl A; Winkler M; Pöhlmann S |
| [Ad] Endereço: | Infection Biology Unit, German Primate Center, Kellnerweg 4, Göttingen, Germany. |
| [Ti] Título: | The glycoprotein of vesicular stomatitis virus promotes release of virus-like particles from tetherin-positive cells. |
| [So] Source: | PLoS One;12(12):e0189073, 2017. |
| [Is] ISSN: | 1932-6203 |
| [Cp] País de publicação: | United States |
| [La] Idioma: | eng |
| [Ab] Resumo: | Vesicular stomatitis virus (VSV) release from infected cells is inhibited by the interferon (IFN)-inducible antiviral host cell factor tetherin (BST-2, CD317). However, several viruses encode tetherin antagonists and it is at present unknown whether residual VSV spread in tetherin-positive cells is also promoted by a virus-encoded tetherin antagonist. Here, we show that the viral glycoprotein (VSV-G) antagonizes tetherin in transfected cells, although with reduced efficiency as compared to the HIV-1 Vpu protein. Tetherin antagonism did not involve alteration of tetherin expression and was partially dependent on a GXXXG motif in the transmembrane domain of VSV-G. However, mutation of the GXXXG motif did not modulate tetherin sensitivity of infectious VSV. These results identify VSV-G as a tetherin antagonist in transfected cells but fail to provide evidence for a contribution of tetherin antagonism to viral spread. |
| [Mh] Termos MeSH primário: |
Antígeno 2 do Estroma da Médula Óssea/metabolismo
Vesiculovirus/metabolismo
Proteínas Virais/fisiologia
Vírion/fisiologia
|
| [Mh] Termos MeSH secundário: |
Animais
Antígeno 2 do Estroma da Médula Óssea/imunologia
Linhagem Celular
Proteínas de Ligação ao GTP/imunologia
Seres Humanos
Suínos
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| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Bone Marrow Stromal Antigen 2); 0 (Viral Proteins); EC 3.6.1.- (GTP-Binding Proteins) |
| [Em] Mês de entrada: | 1712 |
| [Cu] Atualização por classe: | 171226 |
[Lr] Data última revisão:
| 171226 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 171208 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.1371/journal.pone.0189073 |
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