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[PMID]:29329332
[Au] Autor:Goldman SM; Henderson BEP; Walters TJ; Corona BT
[Ad] Endereço:United States Army Institute of Surgical Research, JBSA Fort Sam Houston, Texas, United States of America.
[Ti] Título:Co-delivery of a laminin-111 supplemented hyaluronic acid based hydrogel with minced muscle graft in the treatment of volumetric muscle loss injury.
[So] Source:PLoS One;13(1):e0191245, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Minced muscle autografting mediates de novo myofiber regeneration and promotes partial recovery of neuromuscular strength after volumetric muscle loss injury (VML). A major limitation of this approach is the availability of sufficient donor tissue for the treatment of relatively large VMLs without inducing donor site morbidity. This study evaluated a laminin-111 supplemented hyaluronic acid based hydrogel (HA+LMN) as a putative myoconductive scaffolding to be co-delivered with minced muscle grafts. In a rat tibialis anterior muscle VML model, delivery of a reduced dose of minced muscle graft (50% of VML defect) within HA+LMN resulted in a 42% improvement of peak tetanic torque production over unrepaired VML affected limbs. However, the improvement in strength was not improved compared to a 50% minced graft-only control group. Moreover, histological analysis revealed that the improvement in in vivo functional capacity mediated by minced grafts in HA+LMN was not accompanied by a particularly robust graft mediated regenerative response as determined through donor cell tracking of the GFP+ grafting material. Characterization of the spatial distribution and density of macrophage and satellite cell populations indicated that the combination therapy damps the heightened macrophage response while re-establishing satellite content 14 days after VML to a level consistent with an endogenously healing ischemia-reperfusion induced muscle injury. Moreover, regional analysis revealed that the combination therapy increased satellite cell density mostly in the remaining musculature, as opposed to the defect area. Based on the results, the following salient conclusions were drawn: 1) functional recovery mediated by the combination therapy is likely due to a superposition of de novo muscle fiber regeneration and augmented repair of muscle fibers within the remaining musculature, and 2) The capacity for VML therapies to augment regeneration and repair within the remaining musculature may have significant clinical impact and warrants further exploration.
[Mh] Termos MeSH primário: Ácido Hialurônico/administração & dosagem
Laminina/administração & dosagem
Músculo Esquelético/lesões
Músculo Esquelético/transplante
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Hidrogéis
Masculino
Força Muscular
Músculo Esquelético/fisiologia
Isoformas de Proteínas/administração & dosagem
Ratos
Ratos Endogâmicos Lew
Regeneração/efeitos dos fármacos
Regeneração/fisiologia
Traumatismo por Reperfusão/terapia
Tecidos Suporte/química
Transplante Autólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hydrogels); 0 (Laminin); 0 (Protein Isoforms); 9004-61-9 (Hyaluronic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191245


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[PMID]:29395078
[Au] Autor:Han S; Lee JY; Heo EY; Kwon IK; Yune TY; Youn I
[Ad] Endereço:Biomedical Research Institute, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02791, Republic of Korea.
[Ti] Título:Implantation of a Matrigel-loaded agarose scaffold promotes functional regeneration of axons after spinal cord injury in rat.
[So] Source:Biochem Biophys Res Commun;496(3):785-791, 2018 02 12.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An agarose scaffold can be useful for supporting and guiding injured axons after spinal cord injury (SCI), but the electrophysiological signal of regenerated axon in scaffolds has not yet been determined. The current study investigated whether a Matrigel-loaded agarose scaffold would enhance the regeneration of axons after SCI. Moreover, the functional connectivity of regenerated axons within the channels of the scaffold was evaluated by directly recording motor evoked potentials. Our data showed that the agarose scaffold containing Matrigel can support and enhance linearly organized axon regeneration after SCI. Additionally, motor evoked potentials were successfully recorded from regenerated axons. These results demonstrate that an agarose scaffold loaded with Matrigel could promote the regeneration of axons and guide the reconnection of functional axons after SCI.
[Mh] Termos MeSH primário: Axônios/patologia
Colágeno/química
Regeneração Tecidual Guiada/instrumentação
Laminina/química
Regeneração Nervosa/fisiologia
Proteoglicanas/química
Traumatismos da Medula Espinal/patologia
Traumatismos da Medula Espinal/terapia
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Materiais Biomiméticos/síntese química
Combinação de Medicamentos
Desenho de Equipamento
Análise de Falha de Equipamento
Masculino
Crescimento Neuronal
Próteses e Implantes
Ratos
Ratos Sprague-Dawley
Recuperação de Função Fisiológica
Sefarose/química
Traumatismos da Medula Espinal/fisiopatologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drug Combinations); 0 (Laminin); 0 (Proteoglycans); 119978-18-6 (matrigel); 9007-34-5 (Collagen); 9012-36-6 (Sepharose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180204
[St] Status:MEDLINE


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[PMID]:27770219
[Au] Autor:Marshall LE; Koomullil R; Frost AR; Berry JL
[Ad] Endereço:Department of Biomedical Engineering, University of Alabama at Birmingham, Shelby Biomedical Research Bldg. Rm. 802, 1825 University Blvd, Birmingham, AL, 35294, USA.
[Ti] Título:Computational and Experimental Analysis of Fluid Transport Through Three-Dimensional Collagen-Matrigel Hydrogels.
[So] Source:Ann Biomed Eng;45(4):1027-1038, 2017 04.
[Is] ISSN:1573-9686
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A preclinical testing model for cancer therapeutics that replicates in vivo physiology is needed to accurately describe drug delivery and efficacy prior to clinical trials. To develop an in vitro model of breast cancer that mimics in vivo drug/nutrient delivery as well as physiological size and bio-composition, it is essential to describe the mass transport quantitatively. The objective of the present study was to develop in vitro and computational models to measure mass transport from a perfusion system into a 3D extracellular matrix (ECM). A perfusion-flow bioreactor system was used to control and quantify the mass transport of a macromolecule within an ECM hydrogel with embedded through-channels. The material properties, fluid mechanics, and structure of the construct quantified in the in vitro model were input into, and served as validation of, the computational fluid dynamics (CFD) simulation. Results showed that advection and diffusion played a complementary role in mass transport. As the CFD simulation becomes more complex with embedded blood vessels and cancer cells, it will become more recapitulative of in vivo breast cancers. This study is a step toward development of a preclinical testing platform that will be more predictive of patient response to therapeutics than two-dimensional cell culture.
[Mh] Termos MeSH primário: Neoplasias da Mama
Colágeno
Simulação por Computador
Hidrogéis
Laminina
Modelos Biológicos
Neovascularização Patológica
Proteoglicanas
[Mh] Termos MeSH secundário: Transporte Biológico Ativo
Neoplasias da Mama/irrigação sanguínea
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Combinação de Medicamentos
Feminino
Seres Humanos
Hidrodinâmica
Neovascularização Patológica/metabolismo
Neovascularização Patológica/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Drug Combinations); 0 (Hydrogels); 0 (Laminin); 0 (Proteoglycans); 119978-18-6 (matrigel); 9007-34-5 (Collagen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1007/s10439-016-1748-6


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[PMID]:29369589
[Au] Autor:Karimov DD; Erdman VV; Nasibullin TR; Tuktarova IA; Somova RS; Timasheva YR; Mustafina OE
[Ti] Título:[Alu insertion-deletion polymorphism of COL13A1 and LAMA2 genes: The analysis of association with longevity].
[So] Source:Genetika;52(10):1185-93, 2016 Oct.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The distribution of allele and genotype frequencies of Alu(I/D) polymorphic sites in the COL13A1 and LAMA2 genes coding extracellular matrix protein subunits was characterized in an ethnically homogeneous group (Tatars from the Republic of Bashkortostan, Russia). It was established that the frequency of individuals with the COL13A1*D/*D genotype was higher in the senile age period. The LAMA2*I/*D genotype was predisposing to longevity among women. According to the observed results, the frequency of the LAMA2*I/*D genotype was increased in senile individuals older than 90 years. The observed associations can be explained on the basis of the contemporary view by the importance of Alu elements in gene expression regulation at transcriptional and post-transcriptional levels, the involvement of collagen and laminin in maintaining the structure and function of the extracellular matrix, and the relationship between the extracellular matrix state, pathological changes and aging.
[Mh] Termos MeSH primário: Elementos Alu
Colágeno/genética
Mutação INDEL
Laminina/genética
Longevidade/genética
Polimorfismo Genético
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Colágeno/biossíntese
Feminino
Regulação da Expressão Gênica
Seres Humanos
Laminina/biossíntese
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Laminin); 0 (laminin alpha 2); 9007-34-5 (Collagen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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[PMID]:29198708
[Au] Autor:Lepage M; Seltana A; Thibault MP; Tremblay É; Beaulieu JF
[Ad] Endereço:Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec, Canada.
[Ti] Título:Knockdown of laminin α5 stimulates intestinal cell differentiation.
[So] Source:Biochem Biophys Res Commun;495(1):1510-1515, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Interactions between cells and the extracellular matrix regulate a wide range of cell processes such as proliferation and differentiation. Laminins are major components of the basement membrane that actively participate in most biological functions via their interactions with a variety of specific cell receptors. The α5-containing laminins (LAMA5) are one of the three main types of laminins identified at the epithelial basal lamina in the adult intestine. The aim of the present study was to investigate the role of α5-containing laminins on intestinal cell proliferation and differentiation. Using an shRNA targeting approach, the effects of knocking down the expression of LAMA5 were investigated in the enterocytic-like Caco-2/15 cell line, a well-characterized model for intestinal cell differentiation. Surprisingly, the abolition of the laminin α5 chain resulted in a drastic increase in the differentiation marker sucrase-isomaltase which was correctly expressed at the apical pole of the cells as observed by indirect immunofluorescence. Transient increases of dipeptidylpeptidase IV, villin, CDX2, HNF-1α, HNF-4α and transepithelial resistance as well as an apparent redistribution of the junctional components ZO-1 and E-cadherin were also observed at early stages of differentiation but no specific effect was observed on cell proliferation as evaluated by BrdU incorporation. Taken together, these data suggest that α5-containing laminins repress intestinal differentiation in its early stages.
[Mh] Termos MeSH primário: Diferenciação Celular/fisiologia
Enterócitos/citologia
Enterócitos/fisiologia
Proteínas da Matriz Extracelular/metabolismo
Intestinos/citologia
Laminina/metabolismo
[Mh] Termos MeSH secundário: Animais
Células CACO-2
Proliferação Celular/fisiologia
Técnicas de Silenciamento de Genes
Seres Humanos
Intestinos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (Laminin); 0 (laminin alpha5)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


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[PMID]:29232694
[Au] Autor:Perugini V; Meikle ST; Guildford AL; Santin M
[Ad] Endereço:Centre for Regenerative Medicine and Devices, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, United Kingdom.
[Ti] Título:Hyperbranched poly(ϵ-lysine) substrate presenting the laminin sequence YIGSR induces the formation of spheroids in adult bone marrow stem cells.
[So] Source:PLoS One;12(12):e0187182, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Unlike the fibroblast-like cells formed upon monolayer culture of human mesenchymal stem cells, the natural stem cell niche of the bone marrow and other types of tissues favours the formation of 3-dimensional (3D) cell clusters. The structuring and biological activity of these clusters are regulated by the contacts established by cells with both the basement membrane and neighbour cells and results in their asymmetric division and the consequent maintenance of both a stem population and a committed progeny. The present work demonstrates the potential of a synthetic substrate to mimic the stem cell niche in vitro. The side amino groups of a linear Poly-L-lysine were modified with hyperbranched poly-(ϵ-lysine) peptides, named as dendrons, tethered with the laminin-mimicking sequence, YIGSR. These dendrons presented the YIGSR sequence at the uppermost molecular branching ensuring a controlled spacing of the bioligand. When used to coat the surface of tissue culture plates in a serum-free in vitro cell culture system, the substrate was able to mimic the most relevant features of the basement membrane of the stem cell niche, i.e. the mesh structure of Collagen Type IV and the availability of laminin bioligands relevant to integrin biorecognition. The substrate biomimetic properties were tested for their ability to support the formation of human bone marrow mesenchymal stem cells (hMSCs) 3D spheroids similar to those observed in the natural stem cell niches and their ability to maintain stem cell pluripotency markers. These features were related to the substrate-specific expression and localisation of (i) cell adhesion receptors (i.e. ß-integrin and N-cadherin), (ii) transcription factors of pluripotency markers and cytoskeleton protein and (iii) regulators of cell migration throughout cell culture passages 2 to 4. The results clearly demonstrate the formation of 3D spheroids starting from the asymmetric division of substrate-adhering spread cells, the clustering of relevant integrins and the expression of specific intracellular pathways controlling cytoskeleton formation suggesting their potential use as a substrate for the handling of stem cells prior to transplantation procedures.
[Mh] Termos MeSH primário: Células-Tronco Hematopoéticas/metabolismo
Laminina/metabolismo
Polilisina/metabolismo
[Mh] Termos MeSH secundário: Adulto
Sequência de Aminoácidos
Adesão Celular
Proliferação Celular
Células Cultivadas
Meios de Cultura Livres de Soro
Células-Tronco Hematopoéticas/citologia
Seres Humanos
Laminina/química
Ligantes
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media, Serum-Free); 0 (Laminin); 0 (Ligands); 25104-18-1 (Polylysine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187182


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[PMID]:27770373
[Au] Autor:Gómez-Contreras P; Ramiro-Díaz JM; Sierra A; Stipp C; Domann FE; Weigel RJ; Lal G
[Ad] Endereço:Division of Surgical Oncology and Endocrine Surgery, Department of Surgery, University of Iowa, 200 Hawkins Drive, 4641 JCP, Iowa City, IA, 52242, USA.
[Ti] Título:Extracellular matrix 1 (ECM1) regulates the actin cytoskeletal architecture of aggressive breast cancer cells in part via S100A4 and Rho-family GTPases.
[So] Source:Clin Exp Metastasis;34(1):37-49, 2017 01.
[Is] ISSN:1573-7276
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:ECM1 overexpression is an independent predictor of poor prognosis in primary breast carcinomas, however the mechanisms by which ECM1 affects tumor progression have not been completely elucidated. ECM1 was silenced in the triple-negative breast cancer cell lines Hs578T and MDAMB231 using siRNA and the cells were evaluated for changes in morphology, migration, invasion and adhesion. Actin cytoskeleton alterations were evaluated by fluorescent staining and levels of activated Rho GTPases by pull down assays. ECM1 downregulation led to significantly diminished cell migration (p = 0.0005 for Hs578T and p = 0.02 for MDAMB231) and cell adhesion (p < 0.001 for Hs578T and p = 0.01 for MDAMB231). Cell invasion (matrigel) was reduced only in the Hs578T cells (p < 0.01). Silencing decreased the expression of the prometastatic molecules S100A4 and TGFßR2 in both cell lines and CD44 in Hs578T cells. ECM1-silenced cells also exhibited alterations in cell shape and showed bundles of F-actin across the cell (stress fibers) whereas NT-siRNA treated cells showed peripheral membrane ruffling. Downregulation of ECM1 was also associated with an increased F/G actin ratio, when compared to the cells transfected with NT siRNA (p < 0.001 for Hs578T and p < 0.00035 for MDAMB231) and a concomitant decline of activated Rho A in the Hs578T cells. Re-expression of S100A4 in ECM1-silenced cells rescued the phenotype in the Hs578T cells but not the MDAMB231 cells. We conclude that ECM1 is a key player in the metastatic process and regulates the actin cytoskeletal architecture of aggressive breast cancer cells at least in part via alterations in S100A4 and Rho A.
[Mh] Termos MeSH primário: Proteínas da Matriz Extracelular/genética
Proteínas Serina-Treonina Quinases/biossíntese
Receptores de Fatores de Crescimento Transformadores beta/biossíntese
Proteína A4 de Ligação a Cálcio da Família S100/biossíntese
Neoplasias de Mama Triplo Negativas/genética
[Mh] Termos MeSH secundário: Citoesqueleto de Actina/genética
Adesão Celular/genética
Linhagem Celular Tumoral
Movimento Celular/genética
Colágeno
Combinação de Medicamentos
Matriz Extracelular/genética
Proteínas da Matriz Extracelular/biossíntese
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Receptores de Hialuronatos/genética
Laminina
Invasividade Neoplásica/genética
Invasividade Neoplásica/patologia
Proteínas Serina-Treonina Quinases/genética
Proteoglicanas
Receptores de Fatores de Crescimento Transformadores beta/genética
Proteína A4 de Ligação a Cálcio da Família S100/genética
Neoplasias de Mama Triplo Negativas/patologia
Proteínas rho de Ligação ao GTP/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Drug Combinations); 0 (ECM1 protein, human); 0 (Extracellular Matrix Proteins); 0 (Hyaluronan Receptors); 0 (Laminin); 0 (Proteoglycans); 0 (Receptors, Transforming Growth Factor beta); 0 (S100 Calcium-Binding Protein A4); 119978-18-6 (matrigel); 142662-27-9 (S100A4 protein, human); 9007-34-5 (Collagen); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.30 (transforming growth factor-beta type II receptor); EC 3.6.5.2 (rho GTP-Binding Proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1007/s10585-016-9827-5


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[PMID]:29185592
[Au] Autor:Leonel LCPC; Miranda CMFC; Coelho TM; Ferreira GAS; Caãada RR; Miglino MA; Lobo SE
[Ad] Endereço:Setor de Anatomia, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
[Ti] Título:Decellularization of placentas: establishing a protocol.
[So] Source:Braz J Med Biol Res;51(1):e6382, 2017 Nov 17.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for further analyses using histology, scanning electron microscopy, immunofluorescence and DNA quantification. Sodium dodecyl sulfate (SDS) was the most effective detergent for cell removal. Freezing placentas before decellularization required longer periods of incubation in different detergents. Both perfusion and immersion methods were capable of removing cells. Placentas decellularized using Protocol I (1% SDS, 5 mM EDTA, 50 mM TRIS, and 0.5% antibiotic) preserved the ECM structure better, but Protocol I was less efficient to remove cells and DNA content from the ECM than Protocol II (1% SDS, 5 mM EDTA, 0.05% trypsin, and 0.5% antibiotic).
[Mh] Termos MeSH primário: Matriz Extracelular
Feto/citologia
Placenta/citologia
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis
Temperatura Baixa
Colágeno/análise
Cães
Ácido Edético
Feminino
Fibronectinas/análise
Imunofluorescência
Imersão
Laminina/análise
Microscopia Eletrônica de Varredura
Gravidez
Reprodutibilidade dos Testes
Dodecilsulfato de Sódio/farmacologia
Tensoativos/farmacologia
Engenharia Tecidual/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Fibronectins); 0 (Laminin); 0 (Surface-Active Agents); 368GB5141J (Sodium Dodecyl Sulfate); 9007-34-5 (Collagen); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE


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[PMID]:28746726
[Au] Autor:Mackenzie SJ; Yi JL; Singla A; Russell TM; Osterhout DJ; Calancie B
[Ad] Endereço:Department of Neuroscience, Upstate Medical University, Syracuse, New York, USA.
[Ti] Título:Cauda equina repair in the rat: Part 3. Axonal regeneration across Schwann cell-Seeded collagen foam.
[So] Source:Muscle Nerve;57(1):E78-E84, 2018 Jan.
[Is] ISSN:1097-4598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Treatments for patients with cauda equina injury are limited. METHODS: In this study, we first used retrograde labeling to determine the relative contributions of cauda equina motor neurons to intrinsic and extrinsic rat tail muscles. Next, we transected cauda equina ventral roots and proceeded to bridge the proximal and distal stumps with either a type I collagen scaffold coated in laminin (CL) or a collagen-laminin scaffold that was also seeded with Schwann cells (CLSC). Regeneration was assessed by way of serial retrograde labeling. RESULTS: After accounting for the axonal contributions to intrinsic vs. extrinsic tail muscles, we noted a higher degree of double labeling in the CLSC group (58.0 ± 39.6%) as compared with the CL group (27.8 ± 16.0%; P = 0.02), but not the control group (33.5 ± 18.2%; P = 0.10). DISCUSSION: Our findings demonstrate the feasibility of using CLSCs in cauda equina injury repair. Muscle Nerve 57: E78-E84, 2018.
[Mh] Termos MeSH primário: Axônios/fisiologia
Cauda Equina/lesões
Colágeno Tipo I/farmacologia
Regeneração Nervosa/fisiologia
Células de Schwann/fisiologia
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Contagem de Células
Feminino
Laminina/farmacologia
Neurônios Motores
Músculo Esquelético/inervação
Músculo Esquelético/fisiologia
Ratos
Ratos Endogâmicos F344
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type I); 0 (Laminin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1002/mus.25751


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[PMID]:29044412
[Au] Autor:Kálmán M; Tóth L; Szöllosi D; Oszwald E; Mahalek J; Sadeghian S
[Ad] Endereço:Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary.
[Ti] Título:Correlation Between Extravasation and Alterations of Cerebrovascular Laminin and ß-Dystroglycan Immunoreactivity Following Cryogenic Lesions in Rats.
[So] Source:J Neuropathol Exp Neurol;76(11):929-941, 2017 Nov 01.
[Is] ISSN:1554-6578
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The blood-brain barrier becomes "leaky" following lesions. Former studies revealed that following lesions the immunoreactivity of cerebrovascular laminin becomes detectable whereas that of ß-dystroglycan disappears. These alterations may be indicators of glio-vascular decoupling that may result in the impairment of the blood-brain-barrier. This study investigates correlation between the post-lesion extravasation and the above-mentioned immunohistochemical alterations. Following cryogenic lesions, the survival periods lasted 5, 10, 30 minutes, 1 or 12 hours, or 1 day. Some brains were fixed immediately post-lesion. Immunofluorescent reactions were performed in floating sections. The extravasation was detected with immunostaining for plasma fibronectin and rat immunoglobulins. When the survival period was 30 minutes or longer, the area of extravasation corresponded to the area of altered laminin and ß-dystroglycan immunoreactivities. Following immediate fixation some laminin immunoreactivity was already detected. The extravasation seemed to precede this early appearance of laminin immunoreactivity. The ß-dystroglycan immunoreactivity disappeared later. When the extravasation spread into the corpus callosum, vascular laminin immunoreactivity appeared but the ß-dystroglycan immunoreactivity persisted. It seems that extravasation separates the glial and vascular basal laminae, which results in the appearance of laminin immunoreactivity. The disappearance of ß-dystroglycan immunoreactivity is neither a condition nor an inevitable consequence of the 2 other phenomena.
[Mh] Termos MeSH primário: Distroglicanas/análise
Extravasamento de Materiais Terapêuticos e Diagnósticos/patologia
Congelamento/efeitos adversos
Laminina/análise
Lobo Parietal/química
Lobo Parietal/patologia
[Mh] Termos MeSH secundário: Animais
Barreira Hematoencefálica/química
Barreira Hematoencefálica/patologia
Circulação Cerebrovascular/fisiologia
Feminino
Masculino
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Laminin); 146888-27-9 (Dystroglycans)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171019
[St] Status:MEDLINE
[do] DOI:10.1093/jnen/nlx081



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