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  1 / 3165 MEDLINE  
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[PMID]:28873459
[Au] Autor:Cen K; Li B; Lu Y; Zhang S; Wang C
[Ad] Endereço:CAS Key Laboratory of Insect Developmental and Evolutionary Biology, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
[Ti] Título:Divergent LysM effectors contribute to the virulence of Beauveria bassiana by evasion of insect immune defenses.
[So] Source:PLoS Pathog;13(9):e1006604, 2017 Sep.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lysin motif (LysM) containing proteins can bind chitin and are ubiquitous in various organisms including fungi. In plant pathogenic fungi, a few LysM proteins have been characterized as effectors to suppress chitin-induced immunity in plant hosts and therefore contribute to fungal virulence. The effector mechanism is still questioned in fungus-animal interactions. In this study, we found that LysM proteins are also present in animal pathogenic fungi and have evolved divergently. The genome of the insect pathogen Beauveria bassiana encodes 12 LysM proteins, and the genes were differentially transcribed by the fungus when grown in different conditions. Deletion of six genes that were expressed by the fungus growing in insects revealed that two, Blys2 and Blys5, were required for full fungal virulence. Both proteins could bind chitin and Blys5 (containing two LysM domains) could additionally bind chitosan and cellulose. Truncation analysis of Blys2 (containing five LysM domains) indicated that the combination of LysM domains could determine protein-binding affinity and specificity for different carbohydrates. Relative to the wild-type strain, loss of Blys2 or Blys5 could impair fungal propagation in insect hemocoels and lead to the upregulation of antifungal gene in insects. Interestingly, the virulence defects of ΔBlys2 and ΔBlys5 could be fully restored by complementation with the Slp1 effector from the rice blast fungus Magnaporthe oryzae. In contrast to Slp1 and Blys2, Blys5 could potentially protect fungal hyphae against chitinase hydrolysis. The results of this study not only advance the understanding of LysM protein evolution but also establish the effector mechanism of fungus-animal interactions.
[Mh] Termos MeSH primário: Beauveria/imunologia
Genes Fúngicos/imunologia
Magnaporthe/imunologia
Mucoproteínas/isolamento & purificação
[Mh] Termos MeSH secundário: Magnaporthe/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mucoproteins); 0 (lysin, gastropoda)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006604


  2 / 3165 MEDLINE  
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[PMID]:28665039
[Au] Autor:Zhu Q; Mangukiya HB; Mashausi DS; Guo H; Negi H; Merugu SB; Wu Z; Li D
[Ad] Endereço:School of Pharmacy, Shanghai Jiao Tong University, China.
[Ti] Título:Anterior gradient 2 is induced in cutaneous wound and promotes wound healing through its adhesion domain.
[So] Source:FEBS J;284(17):2856-2869, 2017 Sep.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Anterior gradient 2 (AGR2), a member of protein disulfide isomerase (PDI) family, is both located in cytoplasm and secreted into extracellular matrix. The orthologs of AGR2 have been linked to limb regeneration in newt and wound healing in zebrafish. In mammals, AGR2 influences multiple cell signaling pathways in tumor formation and in normal cell functions related to new tissue formation like angiogenesis. However, the function of AGR2 in mammalian wound healing remains unknown. This study aimed to investigate AGR2 expression and its function during skin wound healing and the possible application of external AGR2 in cutaneous wound to accelerate the healing process. Our results showed that AGR2 expression was induced in the migrating epidermal tongue and hyperplastic epidermis after skin excision. Topical application of recombinant AGR2 significantly accelerated wound-healing process by increasing the migration of keratinocytes (Kera.) and the recruitment of fibroblasts (Fibro.) near the wounded area. External AGR2 also promoted the migration of Kera. and Fibro. in vitro in a dose-dependent manner. The adhesion domain of AGR2 was required for the formation of focal adhesions in migrating Fibro., leading to the directional migration along AGR2 gradient. These results indicate that recombinant AGR2 accelerates skin wound healing through regulation of Kera. and Fibro. migration, thus demonstrating its potential utility as an alternative strategy of the therapeutics to accelerate the healing of acute or chronic skin wounds.
[Mh] Termos MeSH primário: Mucoproteínas/genética
Ativação Transcricional
[Mh] Termos MeSH secundário: Animais
Movimento Celular
Células Cultivadas
Epiderme/metabolismo
Epiderme/patologia
Epiderme/fisiopatologia
Fibroblastos/fisiologia
Células Endoteliais da Veia Umbilical Humana/fisiologia
Queratinócitos/fisiologia
Sistema de Sinalização das MAP Quinases
Masculino
Camundongos Endogâmicos BALB C
Mucoproteínas/metabolismo
Domínios Proteicos
Cicatrização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Agr2 protein, mouse); 0 (Mucoproteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1111/febs.14155


  3 / 3165 MEDLINE  
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[PMID]:28637865
[Au] Autor:Munoz-Munoz J; Cartmell A; Terrapon N; Baslé A; Henrissat B; Gilbert HJ
[Ad] Endereço:From the Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom.
[Ti] Título:An evolutionarily distinct family of polysaccharide lyases removes rhamnose capping of complex arabinogalactan proteins.
[So] Source:J Biol Chem;292(32):13271-13283, 2017 Aug 11.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The human gut microbiota utilizes complex carbohydrates as major nutrients. The requirement for efficient glycan degrading systems exerts a major selection pressure on this microbial community. Thus, we propose that this microbial ecosystem represents a substantial resource for discovering novel carbohydrate active enzymes. To test this hypothesis we screened the potential enzymatic functions of hypothetical proteins encoded by genes of that were up-regulated by arabinogalactan proteins or AGPs. Although AGPs are ubiquitous in plants, there is a paucity of information on their detailed structure, the function of these glycans , and the mechanisms by which they are depolymerized in microbial ecosystems. Here we have discovered a new polysaccharide lyase family that is specific for the l-rhamnose-α1,4-d-glucuronic acid linkage that caps the side chains of complex AGPs. The reaction product generated by the lyase, Δ4,5-unsaturated uronic acid, is removed from AGP by a glycoside hydrolase located in family GH105, producing the final product 4-deoxy-ß-l-threo-hex-4-enepyranosyl-uronic acid. The crystal structure of a member of the novel lyase family revealed a catalytic domain that displays an (α/α) barrel-fold. In the center of the barrel is a deep pocket, which, based on mutagenesis data and amino acid conservation, comprises the active site of the lyase. A tyrosine is the proposed catalytic base in the ß-elimination reaction. This study illustrates how highly complex glycans can be used as a scaffold to discover new enzyme families within microbial ecosystems where carbohydrate metabolism is a major evolutionary driver.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Bacteroides thetaiotaomicron/enzimologia
Loci Gênicos
Modelos Moleculares
Mucoproteínas/metabolismo
Polissacarídeo-Liase/metabolismo
Ramnose/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Biocatálise
Domínio Catalítico
Sequência Conservada
Cristalografia por Raios X
Bases de Dados de Proteínas
Hidrólise
Isoenzimas
Cinética
Filogenia
Proteínas de Plantas/metabolismo
Polissacarídeo-Liase/química
Polissacarídeo-Liase/genética
Conformação Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Estereoisomerismo
Especificidade por Substrato
Tirosina
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Isoenzymes); 0 (Mucoproteins); 0 (Plant Proteins); 0 (Recombinant Proteins); 0 (arabinogalactan proteins); 42HK56048U (Tyrosine); EC 4.2.2.- (Polysaccharide-Lyases); QN34XC755A (Rhamnose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.794578


  4 / 3165 MEDLINE  
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[PMID]:28622512
[Au] Autor:Raj I; Sadat Al Hosseini H; Dioguardi E; Nishimura K; Han L; Villa A; de Sanctis D; Jovine L
[Ad] Endereço:Department of Biosciences and Nutrition and Center for Innovative Medicine, Karolinska Institutet, Huddinge, SE-141 83, Sweden.
[Ti] Título:Structural Basis of Egg Coat-Sperm Recognition at Fertilization.
[So] Source:Cell;169(7):1315-1326.e17, 2017 Jun 15.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1-3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here. Whereas sequence-divergent repeat 1 does not bind lysin, repeat 3 binds it non-species specifically via a high-affinity, largely hydrophobic interface. Due to its intermediate binding affinity, repeat 2 selectively interacts with lysin from the same species. Exposure of a highly positively charged surface of VERL-bound lysin suggests that complex formation both disrupts the organization of egg coat filaments and triggers their electrostatic repulsion, thereby opening a hole for sperm penetration and fusion.
[Mh] Termos MeSH primário: Fertilização
Invertebrados/fisiologia
Vertebrados/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Evolução Biológica
Proteínas do Ovo/química
Proteínas do Ovo/metabolismo
Seres Humanos
Invertebrados/química
Invertebrados/genética
Masculino
Modelos Moleculares
Mucoproteínas/química
Mucoproteínas/metabolismo
Óvulo/química
Óvulo/metabolismo
Alinhamento de Sequência
Especificidade da Espécie
Espermatozoides/química
Espermatozoides/metabolismo
Vertebrados/genética
Difração de Raios X
Glicoproteínas da Zona Pelúcida/química
Glicoproteínas da Zona Pelúcida/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Egg Proteins); 0 (Mucoproteins); 0 (Zona Pellucida Glycoproteins); 0 (lysin, gastropoda)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE


  5 / 3165 MEDLINE  
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[PMID]:28599122
[Au] Autor:Shi T; He Y; Sun W; Wu Y; Li L; Jie Z; Su X
[Ad] Endereço:Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200240, China.
[Ti] Título:Respiratory Syncytial virus infection compromises asthma tolerance by recruiting interleukin-17A-producing cells via CCR6-CCL20 signaling.
[So] Source:Mol Immunol;88:45-57, 2017 Aug.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Asthma tolerance can be induced by breast-feeding or oral feeding with ovalbumin (OVA). Anergy or deletion of specific T cells and generation of T regulatory cells might contribute to this process. However, whether respiratory syncytial virus (RSV) infection would affect asthma tolerance is not very clear. Here, we first established asthma and oral tolerance mouse models and then analyzed airway hypersensitivity and asthma-related genes in the lung, CCR6-expressing IL-17A cells in the lungs, hilar or mesenteric lymph nodes (HLN or MLN) among control, asthmatic, tolerized, RSV infection, and RSV-infected asthmatic and tolerized groups. We also administrated CCL20 or IL-17A neutralizing antibody to RSV-infected tolerized mice to test whether RSV infection would mobilize CCR6-expressing IL-17A cells from MLN to the infected lungs. We found that tolerized mice infected with RSV developed asthma-like responses manifested by increasing airway hypersensitivity, exacerbating peribronchial inflammation, elevating lung asthma-related genes (Il17a, Mu5ac, and Gob5), accumulating CCR6-expressing IL-17A cells in the lungs and HLN with a reduction of this cell population in MLN. CCL20-CCR6 co-expression in RSV-infected tolerized MLN was reduced. Neutralization of CCL20 reduced CD3 CD4 CCR6 cells in the RSV-infected tolerized HLN. Neutralization of IL-17A mitigated the compromising effects of RSV infection on asthma tolerance. Taken together, RSV infection impairs asthma tolerance by recruiting IL-17A-producing cells via CCR6-CCL20 signaling. The findings provide novel insight into exacerbation and therapeutic strategy of asthma under RSV infection.
[Mh] Termos MeSH primário: Asma/imunologia
Quimiocina CCL20/imunologia
Tolerância Imunológica/imunologia
Interleucina-17/imunologia
Receptores CCR6/imunologia
Infecções por Vírus Respiratório Sincicial/imunologia
[Mh] Termos MeSH secundário: Animais
Líquido da Lavagem Broncoalveolar/imunologia
Complexo CD3/metabolismo
Antígenos CD4/metabolismo
Linhagem Celular Tumoral
Canais de Cloreto/genética
Feminino
Imunoglobulina E/sangue
Interleucina-17/genética
Pulmão/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Mucoproteínas/genética
Ovalbumina/imunologia
Infecções por Vírus Respiratório Sincicial/virologia
Vírus Sinciciais Respiratórios/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCL20 protein, mouse); 0 (CCR6 protein, mouse); 0 (CD3 Complex); 0 (CD4 Antigens); 0 (Chemokine CCL20); 0 (Chloride Channels); 0 (Clca3 protein, mouse); 0 (Il17a protein, mouse); 0 (Interleukin-17); 0 (Mucoproteins); 0 (Receptors, CCR6); 37341-29-0 (Immunoglobulin E); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE


  6 / 3165 MEDLINE  
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[PMID]:28254574
[Au] Autor:Bera K; Ray S; Raja W; Ray B
[Ad] Endereço:Natural Products Laboratory, Department of Chemistry, Golapbag, Burdwan, The University of Burdwan, West Bengal 713 104, India.
[Ti] Título:Structural insight of an antioxidative arabinogalactan protein of Aegle marmelos fruit gum and it's interaction with ß-lactoglobulin.
[So] Source:Int J Biol Macromol;99:300-307, 2017 Jun.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The complication of arabinogalactan protein (AGP) structure, a significant ingredient of gum polysaccharides, not merely hinders the allocation of its role, but restricts its utilization as well. Here, we describe structural details of an AGP purified from Aegle marmelos fruit gum. This AGP (310×10 g/mol), which is water-soluble, contains ß-1,3-linked galactopyranosyl main chain substituted at O-6 position with side chains containing galactose and arabinose residues. Also data on sugar composition, ring size, glycosidic linkage pattern, anomeric configuration and sequence of monosaccharide units of a number of oligosaccharides produced from this AGP by chemical and enzymatic methods were acquired. Biochemical analysis reveals resemblance in antioxidative potential between this arabinogalactan protein and standard antioxidants. Moreover, mixture of AGP and ß-lactoglobulin form stable water soluble complex having binding constant K=2.38×10 /M. As gum polysaccharides are important raw materials of food industry discovering an antioxidative gum with the ability of creating stable water soluble complex with ß-lactoglobulin may have important implication.
[Mh] Termos MeSH primário: Aegle/química
Frutas/química
Lactoglobulinas/metabolismo
Mucoproteínas/química
Mucoproteínas/metabolismo
Gomas Vegetais/química
[Mh] Termos MeSH secundário: Hidrólise
Metilação
Peso Molecular
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lactoglobulins); 0 (Mucoproteins); 0 (Plant Gums); 0 (Plant Proteins); 0 (arabinogalactan proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


  7 / 3165 MEDLINE  
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[PMID]:28234963
[Au] Autor:Milewska-Hendel A; Baczewska AH; Sala K; Dmuchowski W; Bragoszewska P; Gozdowski D; Jozwiak A; Chojnacki T; Swiezewska E; Kurczynska E
[Ad] Endereço:Department of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland.
[Ti] Título:Quantitative and qualitative characteristics of cell wall components and prenyl lipids in the leaves of Tilia x euchlora trees growing under salt stress.
[So] Source:PLoS One;12(2):e0172682, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The study was focused on assessing the presence of arabinogalactan proteins (AGPs) and pectins within the cell walls as well as prenyl lipids, sodium and chlorine content in leaves of Tilia x euchlora trees. The leaves that were analyzed were collected from trees with and without signs of damage that were all growing in the same salt stress conditions. The reason for undertaking these investigations was the observations over many years that indicated that there are trees that present a healthy appearance and trees that have visible symptoms of decay in the same habitat. Leaf samples were collected from trees growing in the median strip between roadways that have been intensively salted during the winter season for many years. The sodium content was determined using atomic spectrophotometry, chloride using potentiometric titration and poly-isoprenoids using HPLC/UV. AGPs and pectins were determined using immunohistochemistry methods. The immunohistochemical analysis showed that rhamnogalacturonans I (RG-I) and homogalacturonans were differentially distributed in leaves from healthy trees in contrast to leaves from injured trees. In the case of AGPs, the most visible difference was the presence of the JIM16 epitope. Chemical analyses of sodium and chloride showed that in the leaves from injured trees, the level of these ions was higher than in the leaves from healthy trees. Based on chromatographic analysis, four poly-isoprenoid alcohols were identified in the leaves of T. x euchlora. The levels of these lipids were higher in the leaves from healthy trees. The results suggest that the differences that were detected in the apoplast and symplasm may be part of the defensive strategy of T. x euchlora trees to salt stress, which rely on changes in the chemical composition of the cell wall with respect to the pectic and AGP epitopes and an increased synthesis of prenyl lipids.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Parede Celular/efeitos dos fármacos
Lipídeos/biossíntese
Cloreto de Sódio/farmacologia
Estresse Fisiológico
Terpenos/metabolismo
Tilia/efeitos dos fármacos
[Mh] Termos MeSH secundário: Álcoois/isolamento & purificação
Álcoois/metabolismo
Parede Celular/química
Parede Celular/metabolismo
Lipídeos/isolamento & purificação
Mucoproteínas/biossíntese
Mucoproteínas/isolamento & purificação
Pectinas/biossíntese
Pectinas/isolamento & purificação
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Proteínas de Plantas/biossíntese
Proteínas de Plantas/isolamento & purificação
Salinidade
Solo/química
Terpenos/isolamento & purificação
Tilia/metabolismo
Árvores/efeitos dos fármacos
Árvores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alcohols); 0 (Lipids); 0 (Mucoproteins); 0 (Pectins); 0 (Plant Proteins); 0 (Soil); 0 (Terpenes); 0 (arabinogalactan proteins); 0 (rhamnogalacturonan I); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172682


  8 / 3165 MEDLINE  
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[PMID]:28134080
[Au] Autor:Nagafuchi Y; Shoda H; Sumitomo S; Nakachi S; Kato R; Tsuchida Y; Tsuchiya H; Sakurai K; Hanata N; Tateishi S; Kanda H; Fujio K; Yamamoto K
[Ad] Endereço:Department of Allergy and Rheumatology, Graduate School of Medicine, The University of Tokyo, Japan.
[Ti] Título:Enhanced gut homing receptor expression of unswitched memory B cells in rheumatoid arthritis.
[So] Source:Clin Exp Rheumatol;35(2):354-355, 2017 Mar-Apr.
[Is] ISSN:0392-856X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:****************************************************************************.
[Mh] Termos MeSH primário: Artrite Reumatoide/imunologia
Linfócitos B/imunologia
Microbioma Gastrointestinal/imunologia
Trato Gastrointestinal/imunologia
Memória Imunológica
Integrinas/imunologia
Receptores de Retorno de Linfócitos/imunologia
[Mh] Termos MeSH secundário: Artrite Reumatoide/sangue
Linfócitos B/metabolismo
Estudos de Casos e Controles
Disbiose
Trato Gastrointestinal/metabolismo
Trato Gastrointestinal/microbiologia
Interações Hospedeiro-Patógeno
Seres Humanos
Imunoglobulinas/imunologia
Imunoglobulinas/metabolismo
Integrinas/sangue
Ligantes
Tecido Linfoide/imunologia
Tecido Linfoide/metabolismo
Mucoproteínas/imunologia
Mucoproteínas/metabolismo
Fenótipo
Receptores de Retorno de Linfócitos/sangue
Fator Reumatoide/sangue
Fator Reumatoide/imunologia
Transdução de Sinais
Regulação para Cima
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Immunoglobulins); 0 (Integrins); 0 (Ligands); 0 (MADCAM1 protein, human); 0 (Mucoproteins); 0 (Receptors, Lymphocyte Homing); 0 (integrin alpha4beta7); 9009-79-4 (Rheumatoid Factor)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170131
[St] Status:MEDLINE


  9 / 3165 MEDLINE  
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[PMID]:28122511
[Au] Autor:Sala K; Malarz K; Barlow PW; Kurczynska EU
[Ad] Endereço:Department of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, 28 Jagiellonska St, 40-032, Katowice, Poland. ksala@us.edu.pl.
[Ti] Título:Distribution of some pectic and arabinogalactan protein epitopes during Solanum lycopersicum (L.) adventitious root development.
[So] Source:BMC Plant Biol;17(1):25, 2017 Jan 25.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The adventitious roots (AR) of plants share the same function as primary and lateral roots (LR), although their development is mainly an adaptive reaction to stress conditions. Regeneration of grafted plants is often accompanied by AR formation thus making the grafting technique a good model for studying AR initiation and development and their means of emergence. Pectins and arabinogalactan proteins (AGP) are helpful markers of particular cellular events, such as programmed cell death (PCD), elongation, proliferation or other differentiation events that accompany AR development. However, little is known about the distribution of pectins and AGPs during AR ontogeny, either in the primordium or stem tissues from which AR arise or their correspondence with these events during LR formation. RESULTS: AR were developed from different stem tissues such as parenchyma, xylem rays and the cambium, depending on the stem age and treatment (grafting versus cutting) of the parental tissue. Immunochemical analysis of the presence of pectic (LM8, LM19, LM20) and AGP (JIM8, JIM13, JIM16) epitopes in AR and AR-associated tissues showed differential, tissue-specific distributions of these epitopes. Two pectic epitopes (LM19, LM20) were developmentally regulated and the occurrence of the LM8 xylogalacturonan epitope in the root cap of the AR differed from other species described so far. AGP epitopes were abundantly present in the cytoplasmic compartments (mainly the tonoplast) and were correlated with the degree of cell vacuolisation. JIM8 and JIM13 epitopes were detected in the more advanced stages of primordium development, whereas the JIM16 epitope was present from the earliest division events of the initial AR cells. The comparison between AR and LR showed quantitative (AGP,) and qualitative (pectins) differences. CONCLUSION: The chemical compositions of adventitious and lateral root cells show differences that correlate with the different origins of these cells. In AR, developmental changes in the distribution of pectins and AGP suggest the turnover of wall compounds. Our data extend the knowledge about the distribution of pectin and AGP during non-embryogenic root development in a species that is important from an agronomic point of view.
[Mh] Termos MeSH primário: Lycopersicon esculentum/metabolismo
Mucoproteínas/metabolismo
Raízes de Plantas/metabolismo
[Mh] Termos MeSH secundário: Epitopos/imunologia
Epitopos/metabolismo
Imuno-Histoquímica
Lycopersicon esculentum/anatomia & histologia
Lycopersicon esculentum/crescimento & desenvolvimento
Mucoproteínas/imunologia
Pectinas/metabolismo
Proteínas de Plantas/imunologia
Proteínas de Plantas/metabolismo
Raízes de Plantas/anatomia & histologia
Raízes de Plantas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Epitopes); 0 (Mucoproteins); 0 (Pectins); 0 (Plant Proteins); 0 (arabinogalactan proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-016-0949-3


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[PMID]:27941872
[Au] Autor:Dumartin L; Alrawashdeh W; Trabulo SM; Radon TP; Steiger K; Feakins RM; di Magliano MP; Heeschen C; Esposito I; Lemoine NR; Crnogorac-Jurcevic T
[Ad] Endereço:Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, London, UK.
[Ti] Título:ER stress protein AGR2 precedes and is involved in the regulation of pancreatic cancer initiation.
[So] Source:Oncogene;36(22):3094-3103, 2017 Jun 01.
[Is] ISSN:1476-5594
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mechanisms of initiation of pancreatic ductal adenocarcinoma (PDAC) are still largely unknown. In the present study, we analysed the role of anterior gradient-2 (AGR2) in the earliest stages of pancreatic neoplasia. Immunohistochemical analysis of chronic pancreatitis (CP) and peritumoral areas in PDAC tissues showed that AGR2 was present in tubular complexes (TC) and early pancreatic intraepithelial neoplasia (PanINs). Moreover, AGR2 was also found in discrete subpopulations of non-transformed cells neighbouring these pre-neoplastic lesions. In primary cells derived from human patient-derived xenograft (PDX) model, flow-cytometry revealed that AGR2 was overexpressed in pancreatic cancer stem cells (CSC) compared with non-stem cancer cells. In LSL-Kras ;Pdx1-Cre (KC) mouse model Agr2 induction preceded the formation of pre-neoplastic lesions and their development was largely inhibited by Agr2 deletion in engineered LSL-Kras ;Pdx1-Cre; Agr2 mice. In vitro, AGR2 expression was stimulated by tunicamycin-induced endoplasmic reticulum (ER) stress in both KRAS wild-type normal pancreas cells, as well as in KRAS mutated pancreatic cancer cells and was essential for ER homoeostasis. The unfolded protein response proteins GRP78, ATF6 and XBP1s were found expressed in CP and PDAC peritumoral tissues, but in contrast to AGR2, their expression was switched off during TC and PanIN formation. Real-time PCR and ELISA analyses showed that ER stress induced a pro-inflammatory phenotype in pancreatic normal, cancer and stellate cells. Moreover, AGR2 expression was inducible by paracrine transfer of ER stress and pro-inflammation between different pancreatic cell types. Our findings demonstrate that AGR2 induced in ER-stressed and inflammatory pre-neoplastic pancreas is a potential marker of cancer progenitor cells with an important functional role in PDAC initiation.
[Mh] Termos MeSH primário: Carcinoma Ductal Pancreático/patologia
Estresse do Retículo Endoplasmático/fisiologia
Mucoproteínas/metabolismo
Neoplasias Pancreáticas/patologia
[Mh] Termos MeSH secundário: Animais
Carcinoma Ductal Pancreático/genética
Carcinoma Ductal Pancreático/metabolismo
Transformação Celular Neoplásica/genética
Transformação Celular Neoplásica/metabolismo
Transformação Celular Neoplásica/patologia
Modelos Animais de Doenças
Seres Humanos
Camundongos
Mucoproteínas/biossíntese
Mucoproteínas/deficiência
Mucoproteínas/genética
Neoplasias Pancreáticas/genética
Neoplasias Pancreáticas/metabolismo
Proteínas Proto-Oncogênicas p21(ras)/genética
Proteínas Proto-Oncogênicas p21(ras)/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Agr2 protein, mouse); 0 (Mucoproteins); EC 3.6.5.2 (Kras2 protein, mouse); EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1038/onc.2016.459



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