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[PMID]:28461410
[Au] Autor:Jin C; Kenny DT; Skoog EC; Padra M; Adamczyk B; Vitizeva V; Thorell A; Venkatakrishnan V; Lindén SK; Karlsson NG
[Ad] Endereço:From the ‡Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Box 440, Medicinaregatan 9A, 405 30 Gothenburg, Sweden.
[Ti] Título:Structural Diversity of Human Gastric Mucin Glycans.
[So] Source:Mol Cell Proteomics;16(5):743-758, 2017 May.
[Is] ISSN:1535-9484
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mucin -glycosylation of 10 individuals with and without gastric disease was examined in depth in order to generate a structural map of human gastric glycosylation. In the stomach, these mucins and their -glycosylation protect the epithelial surface from the acidic gastric juice and provide the first point of interaction for pathogens such as reported to cause gastritis, gastric and duodenal ulcers and gastric cancer. The rational of the present study was to map the -glycosylation that the pathogen may come in contact with. An enormous diversity in glycosylation was found, which varied both between individuals and within mucins from a single individual: mucin glycan chain length ranged from 2-13 residues, each individual carried 34-103 -glycan structures and in total over 258 structures were identified. The majority of gastric -glycans were neutral and fucosylated. Blood group I antigens, as well as terminal α1,4-GlcNAc-like and GalNAcß1-4GlcNAc-like (LacdiNAc-like), were common modifications of human gastric -glycans. Furthemore, each individual carried 1-14 glycan structures that were unique for that individual. The diversity and alterations in gastric -glycosylation broaden our understanding of the human gastric -glycome and its implications for gastric cancer research and emphasize that the high individual variation makes it difficult to identify gastric cancer specific structures. However, despite the low number of individuals, we could verify a higher level of sialylation and sulfation on gastric -glycans from cancerous tissue than from healthy stomachs.
[Mh] Termos MeSH primário: Mucinas Gástricas/química
Polissacarídeos/química
[Mh] Termos MeSH secundário: Antígenos de Grupos Sanguíneos/química
Cromatografia Líquida
Epitopos/metabolismo
Mucinas Gástricas/metabolismo
Seres Humanos
Mucina-5AC/química
Mucina-5AC/metabolismo
Polissacarídeos/metabolismo
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Group Antigens); 0 (Epitopes); 0 (Gastric Mucins); 0 (Mucin 5AC); 0 (Polysaccharides)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1074/mcp.M116.067983


  2 / 714 MEDLINE  
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[PMID]:29073245
[Au] Autor:Scott I; Umair S; Savoian MS; Simpson HV
[Ad] Endereço:Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand.
[Ti] Título:Abomasal dysfunction and cellular and mucin changes during infection of sheep with larval or adult Teladorsagia circumcincta.
[So] Source:PLoS One;12(10):e0186752, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This is the first integrated study of the effects on gastric secretion, inflammation and fundic mucins after infection with L3 T. circumcincta and in the very early period following transplantation of adult worms. At 3 months-of-age, 20 Coopworth lambs were infected intraruminally with 35,000 L3; infected animals were killed on Days 5, 10, 15, 20 and 30 post-infection and 6 controls on either Day 0 or 30 post-infection. Another 15 Romney cross lambs received 10,000 adult worms at 4-5 months-of-age though surgically-implanted abomasal cannulae and were killed after 6, 12, 24 and 72 hours; uninfected controls were also killed at 72 hours. Blood was collected at regular intervals from all animals for measurement of serum gastrin and pepsinogen and abomasal fluid for pH measurement from cannulated sheep. Tissues collected at necropsy were fixed in Bouin's fluid for light microscopy, immunocytochemistry and mucin staining and in Karnovsky's fluid for electron microscopy. Nodules around glands containing developing larvae were seen on Day 5 p.i., but generalised effects on secretion occurred only after parasite emergence and within hours after transplantation of adult worms. After L3 infection, there were maximum worm burdens on Days 10-15 post-infection, together with peak tissue eosinophilia, inhibition of gastric acid secretion, hypergastrinaemia, hyperpepsinogenaemia, loss of parietal cells, enlarged gastric pits containing less mucin and increased numbers of mucous neck cells. After adult transplantation, serum pepsinogen was significantly increased after 9 hours and serum gastrin after 18 hours. Parallel changes in host tissues and the numbers of parasites in the abomasal lumen suggest that luminal parasites, but not those in the tissues, are key drivers of the pathophysiology and inflammatory response in animals exposed to parasites for the first time. These results are consistent with initiation of the host response by parasite chemicals diffusing across the surface epithelium, possibly aided by components of ES products which increased permeability. Parietal cells appear to be a key target, resulting in secondary increases in serum gastrin, pit elongation, loss of surface mucins and inhibition of chief cell maturation. Inflammation occurs in parallel, and could either cause the pathology or exacerbate the direct effects of ES products.
[Mh] Termos MeSH primário: Mucinas Gástricas/secreção
Doenças dos Ovinos
Ovinos
Gastropatias
Estômago de Ruminante
Trichostrongyloidea
Tricostrongiloidíase
[Mh] Termos MeSH secundário: Animais
Gastrinas/sangue
Larva
Pepsinogênio A/sangue
Ovinos/sangue
Ovinos/parasitologia
Doenças dos Ovinos/sangue
Doenças dos Ovinos/parasitologia
Gastropatias/sangue
Gastropatias/parasitologia
Estômago de Ruminante/parasitologia
Estômago de Ruminante/secreção
Tricostrongiloidíase/sangue
Tricostrongiloidíase/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gastric Mucins); 0 (Gastrins); 9001-10-9 (Pepsinogen A)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186752


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[PMID]:28376132
[Au] Autor:Mitsuishi T; Hamatani S; Hirooka S; Fukasawa N; Aizawa D; Hara Y; Dobashi A; Goda K; Fukuda T; Saruta M; Urashima M; Ikegami M
[Ad] Endereço:Department of Pathology, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.
[Ti] Título:Clinicopathological characteristics of duodenal epithelial neoplasms: Focus on tumors with a gastric mucin phenotype (pyloric gland-type tumors).
[So] Source:PLoS One;12(4):e0174985, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Epithelial tumors less commonly occur in the duodenum than in the stomach or large intestine. The clinicopathological characteristics of duodenal epithelial tumors remain a matter of debate. We therefore studied resected specimens to investigate the clinicopathological characteristics of duodenal epithelial tumors. MATERIALS AND METHODS: Among duodenal epithelial tumors resected endoscopically or surgically in our hospital, we studied the clinicopathological characteristics of 110 adenomas or intramucosal carcinomas. The grade of atypia of all tumors was classified into 3 groups according to the World Health Organization (WHO) 2010 classification. The tumors were immunohistochemically evaluated to determine the frequency of differentiation toward fundic glands. RESULTS: As for patient characteristics, there were 76 men (75.2%) and 25 women (24.8%), with a median age of 65 years (range, 34 to 84). The tumors most commonly arose in the first to second part of the duodenum. Many lesions were flat, and the median tumor diameter was 8.0 mm. The lesions were classified into 2 types according to mucin phenotype: intestinal-type tumors (98 lesions, 89.1%) and gastric-type tumors (12 lesions, 10.9%). Intestinal-type tumors were subdivided into 2 groups: tubular-type tumors (91 lesions, 82.7%) and tubulovillous-type tumors (7 lesions, 6.4%). Gastric-type tumors were classified into 2 types: foveolar type (3 lesions, 2.7%) and pyloric gland-type (PG) tumors (9 lesions, 8.2%). The grade of atypia was significantly higher in gastric-type tumors (p<0.01). PG tumors were gastric-type tumors characterized by pyloric glands and findings suggesting differentiation toward fundic glands. CONCLUSIONS: About 10% of the duodenal tumors had a gastric-type mucin phenotype. Gastric-type tumors showed high-grade atypia. In particular, PG tumors showed similarities to PG tumors of the stomach, such as differentiation toward fundic glands.
[Mh] Termos MeSH primário: Neoplasias Duodenais/patologia
[Mh] Termos MeSH secundário: Adenocarcinoma Mucinoso/metabolismo
Adenocarcinoma Mucinoso/patologia
Adenoma/metabolismo
Adenoma/patologia
Adulto
Idoso
Idoso de 80 Anos ou mais
Carcinoma/metabolismo
Carcinoma/patologia
Neoplasias Duodenais/classificação
Neoplasias Duodenais/metabolismo
Feminino
Mucinas Gástricas/metabolismo
Mucosa Gástrica/metabolismo
Mucosa Gástrica/patologia
ATPase Trocadora de Hidrogênio-Potássio/metabolismo
Seres Humanos
Masculino
Meia-Idade
Pepsinogênio A/metabolismo
Neoplasias Gástricas/metabolismo
Neoplasias Gástricas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gastric Mucins); 9001-10-9 (Pepsinogen A); EC 3.6.3.10 (H(+)-K(+)-Exchanging ATPase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174985


  4 / 714 MEDLINE  
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[PMID]:28088838
[Au] Autor:Hayakawa M; Nishikura K; Ajioka Y; Aoyagi Y; Terai S
[Ad] Endereço:Division of Molecular and Diagnostic Pathology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
[Ti] Título:Re-evaluation of Phenotypic Expression in Differentiated-type Early Adenocarcinoma of the Stomach.
[So] Source:Pathol Int;67(3):131-140, 2017 Mar.
[Is] ISSN:1440-1827
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:A total of 313 cases of differentiated-type early gastric adenocarcinomas, including 113 cases of small-sized carcinoma (5< × ≤10 mm) and 121 cases of microcarcinoma (0< × ≤5 mm), were examined immunohistochemically to clarify the phenotypic expressions. They were classified into four categories (gastric phenotype (G-type), intestinal phenotype, gastrointestinal phenotype, and null phenotype) by a two-step process: the phenotype based on an immunoprofile of mucin core proteins (MUCs) with CDX2 (w/.CDX2-assessment); and the phenotype of MUCs only (w/o.CDX2-assessment). CDX2 expression was observed in 89.1% (279/313); it was highly expressed in 87.6% (106/121) of microcarcinomas. MUC2 expression increased as tumor size increased (P < 0.05). Compared with w/o.CDX2-assessment, w/.CDX2-assessment showed significantly fewer G-type carcinomas (P < 0.05). Each phenotype marker was less expressed in the submucosal part than in the mucosal part. In conclusion, CDX2 was a sensitive marker for assessing intestinal phenotype. A large portion of the early differentiated-type adenocarcinomas expressed CDX2 from the very early stage of carcinogenesis, and the proportion of G-type was unexpectedly low. Lower expression of each phenotype marker was considered the cause of phenotype alteration during submucosal invasion.
[Mh] Termos MeSH primário: Adenocarcinoma/classificação
Adenocarcinoma/patologia
Biomarcadores Tumorais/análise
Neoplasias Gástricas/classificação
Neoplasias Gástricas/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Fator de Transcrição CDX2/análise
Fator de Transcrição CDX2/biossíntese
Feminino
Mucinas Gástricas/análise
Mucinas Gástricas/biossíntese
Seres Humanos
Imuno-Histoquímica
Masculino
Meia-Idade
Fenótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (CDX2 Transcription Factor); 0 (CDX2 protein, human); 0 (Gastric Mucins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170116
[St] Status:MEDLINE
[do] DOI:10.1111/pin.12506


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[PMID]:27919816
[Au] Autor:McColl J; Horvath R; Yakubov GE; Ramsden JJ
[Ad] Endereço:Department of Materials, Cranfield University, Bedfordshire, MK43 0AL, UK; Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK.
[Ti] Título:Surface rearrangement of adsorbed EGCG-mucin complexes on hydrophilic surfaces.
[So] Source:Int J Biol Macromol;95:704-712, 2017 Feb.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The kinetic adsorption-desorption behaviour of porcine gastric mucin in the presence of physiologically relevant concentrations of the polyphenol epigallocatechin gallate (EGCG) was investigated using high-resolution kinetic optical waveguide lightmode spectroscopy (OWLS) and atomic force microscopy (AFM). Comparison with dynamic light scattering results from EGCG-mucin mixtures indicates that discrete particles are formed whose size increases with increasing EGCG:mucin ratio. These particles are deduced to be the adsorbing entities, which fuse on the surface to form complex surface layers. At low molar EGCG:mucin ratios (<∼1000), aggregates fuse on the surface to form a monolayer similar to one of pure mucin. With increasing EGCG concentration, the surface assembly of aggregates becomes consistent with their rearrangement and spreading in the shape of a spherical segment. At the highest molar ratios investigated (>12,000) the particles begin to destabilize. The presence of EGCG leads to birefringence hysteresis during adsorption-desorption, indicating structural rearrangement, even at molar ratios ∼1000. The intensification of the phenomenon with increasing EGCG:mucin ratio mimics what was previously observed with the increase of mucin concentration in an EGCG-free system.
[Mh] Termos MeSH primário: Catequina/análogos & derivados
Mucinas Gástricas/química
Interações Hidrofóbicas e Hidrofílicas
[Mh] Termos MeSH secundário: Adsorção
Catequina/química
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gastric Mucins); 8R1V1STN48 (Catechin); BQM438CTEL (epigallocatechin gallate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161207
[St] Status:MEDLINE


  6 / 714 MEDLINE  
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[PMID]:27599458
[Au] Autor:Nudelman R; Gloukhikh E; Rekun A; Richter S
[Ad] Endereço:School of Chemistry Faculty of Exact Sciences, Tel- Aviv University, Tel-Aviv, Israel.
[Ti] Título:Investigation of the pH-dependence of dye-doped protein-protein interactions.
[So] Source:Protein Sci;25(11):1918-1923, 2016 Nov.
[Is] ISSN:1469-896X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Proteins can dramatically change their conformation under environmental conditions such as temperature and pH. In this context, Glycoprotein's conformational determination is challenging. This is due to the variety of domains which contain rich chemical characters existing within this complex. Here we demonstrate a new, straightforward and efficient technique that uses the pH-dependent properties of dyes-doped Pig Gastric Mucin (PGM) for predicting and controlling protein-protein interaction and conformation. We utilize the PGM as natural host matrix which is capable of dynamically changing its conformational shape and adsorbing hydrophobic and hydrophilic dyes under different pH conditions and investigate and control the fluorescent properties of these composites in solution. It is shown at various pH conditions, a large variety of light emission from these complexes such as red, green and white is obtained. This phenomenon is explained by pH-dependent protein folding and protein-protein interactions that induce different emission spectra which are mediated and controlled by means of dye-dye interactions and surrounding environment. This process is used to form the technologically challenging white light-emitting liquid or solid coating for LED devices.
[Mh] Termos MeSH primário: Corantes Fluorescentes/química
Mucinas Gástricas/química
Dobramento de Proteína
[Mh] Termos MeSH secundário: Animais
Concentração de Íons de Hidrogênio
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Gastric Mucins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.1002/pro.3021


  7 / 714 MEDLINE  
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[PMID]:27568354
[Au] Autor:Oh S; Borrós S
[Ad] Endereço:Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, 08017, Spain; Sagetis-Biotech, Barcelona, Spain.
[Ti] Título:Mucoadhesion vs mucus permeability of thiolated chitosan polymers and their resulting nanoparticles using a quartz crystal microbalance with dissipation (QCM-D).
[So] Source:Colloids Surf B Biointerfaces;147:434-441, 2016 Nov 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this present study was to evaluate the combination properties between mucoadhesion/mucus permeability of thiolated chitosans (TC) and their resulting nanoparticles using a quartz crystal microbalance with dissipation (QCM-D). The QCM-D experiments were conducted at pH 4 or 6.8 to assess the interaction between thiolated polymers, with low (TCL), medium (TCM) and high (TCH) contents of free thiol groups, and native porcine gastric mucin (NPGM). TCL was chosen for further carriers as it showed higher permeability into the NPGM layer compared to TCM and TCH. In this study, we describe a formulation of a novel carrier comprised by positively charged TCL, negatively charged DNA and degradable oligopeptide-modified poly(ß-amino ester)s (PBAEs), which were employed in order to approach for tuning particle size and surface charge of complexes. TCL/PBAE complexes with or without DNA were characterized using dynamic light scattering. Mechanism of adsorption or permeation of the TCL/PBAE/DNA complexes into the NPGM barrier was investigated with QCM-D, which is a highly sensitive technique for studying nanomechanical (viscoelastic) changes of the substrates. This work might provide that the QCM-D technique would be a promising method to monitor the dynamic behaviour between complexes and NPGM.
[Mh] Termos MeSH primário: Quitosana/metabolismo
Mucinas Gástricas/metabolismo
Muco/metabolismo
Nanopartículas/química
Polímeros/metabolismo
Técnicas de Microbalança de Cristal de Quartzo/métodos
Compostos de Sulfidrila/química
[Mh] Termos MeSH secundário: Adesividade
Adsorção
Animais
Quitosana/química
Mucinas Gástricas/química
Muco/química
Tamanho da Partícula
Permeabilidade
Polímeros/química
Propriedades de Superfície
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gastric Mucins); 0 (Polymers); 0 (Sulfhydryl Compounds); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160829
[St] Status:MEDLINE


  8 / 714 MEDLINE  
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[PMID]:27563024
[Au] Autor:Yoo KS; Choi HS; Jun DW; Lee HL; Lee OY; Yoon BC; Lee KG; Paik SS; Kim YS; Lee J
[Ad] Endereço:Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
[Ti] Título:MUC Expression in Gallbladder Epithelial Tissues in Cholesterol-Associated Gallbladder Disease.
[So] Source:Gut Liver;10(5):851-8, 2016 Sep 15.
[Is] ISSN:2005-1212
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Gallstone pathogenesis is linked to mucin hypersecretion and bacterial infection. Several mucin genes have been identified in gallbladder epithelial cells (GBECs). We investigated MUC expression in cholesterol-associated gallbladder disease and evaluated the relationship between mucin and bacterial infection. METHODS: The present study involved 20 patients with cholesterol stones with cholecystitis, five with cholesterol stones with cholesterolosis, six with cholesterol polyps, two with gallbladder cancer, and six controls. Canine GBECs treated with lipopolysaccharide were also studied. MUC3, MUC5AC, MUC5B, and MUC6 antibodies were used for dot/slot immunoblotting and immunohistochemical studies of the gallbladder epithelial tissues, canine GBECs, and bile. Reverse-transcription polymerase chain reaction was performed to evaluate MUC3 and MUC5B expression. RESULTS: MUC3, MUC5AC, MUC5B, and MUC6 were expressed in the normal gallbladder epithelium, and of those, MUC3 and MUC5B exhibited the highest expression levels. Greatly increased levels of MUC3 and MUC5B expression were observed in the cholesterol stone group, and slightly increased levels were observed in the cholesterol polyp group; MUC3 and MUC5B mRNA was also upregulated in those groups. Canine GBECs treated with lipopolysaccharide also showed upregulation of MUC3 and MUC5B. CONCLUSIONS: The mucin genes with the highest expression levels in gallbladder tissue in cholesterol-associated diseases were MUC3 and MUC5B. Cholesterol stones and gallbladder infections were associated with increased MUC3 and MUC5B expression.
[Mh] Termos MeSH primário: Colecistite/metabolismo
Células Epiteliais/metabolismo
Doenças da Vesícula Biliar/metabolismo
Mucinas Gástricas/metabolismo
Hipercolesterolemia/metabolismo
[Mh] Termos MeSH secundário: Animais
Estudos de Casos e Controles
Colecistite/etiologia
Cães
Vesícula Biliar/citologia
Doenças da Vesícula Biliar/etiologia
Seres Humanos
Hipercolesterolemia/complicações
Mucina-5AC/metabolismo
Mucina-3/metabolismo
Mucina-5B/metabolismo
Mucina-6/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gastric Mucins); 0 (Mucin 5AC); 0 (Mucin-3); 0 (Mucin-5B); 0 (Mucin-6)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160827
[St] Status:MEDLINE
[do] DOI:10.5009/gnl15600


  9 / 714 MEDLINE  
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[PMID]:27474724
[Au] Autor:Lou F; DiCaprio E; Li X; Dai X; Ma Y; Hughes J; Chen H; Kingsley DH; Li J
[Ad] Endereço:Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA Program in Food Science and Technology, The Ohio State University, Columbus, Ohio, USA.
[Ti] Título:Variable High-Pressure-Processing Sensitivities for Genogroup II Human Noroviruses.
[So] Source:Appl Environ Microbiol;82(19):6037-45, 2016 Oct 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Human norovirus (HuNoV) is a leading cause of foodborne diseases worldwide. High-pressure processing (HPP) is one of the most promising nonthermal technologies for the decontamination of viral pathogens in foods. However, the survival of HuNoVs after HPP is poorly understood because these viruses cannot be propagated in vitro In this study, we estimated the survival of different HuNoV strains within genogroup II (GII) after HPP treatment using viral receptor-binding ability as an indicator. Four HuNoV strains (one GII genotype 1 [GII.1] strain, two GII.4 strains, and one GII.6 strain) were treated at high pressures ranging from 200 to 600 MPa. After treatment, the intact viral particles were captured by porcine gastric mucin-conjugated magnetic beads (PGM-MBs) that contained histo-blood group antigens, the functional receptors for HuNoVs. The genomic RNA copies of the captured HuNoVs were quantified by real-time reverse transcriptase PCR (RT-PCR). Two GII.4 HuNoVs had similar sensitivities to HPP. The resistance of HuNoV strains against HPP ranked as follows: GII.1 > GII.6 > GII.4, with GII.4 being the most sensitive. Evaluation of temperature and matrix effects on HPP-mediated inactivation of HuNoV GII.4, GII.1, and GII.6 strains showed that HuNoV was more easily inactivated at lower temperatures and at a neutral pH. In addition, phosphate-buffered saline (PBS) and minimal essential medium (MEM) can provide protective effects against HuNoV inactivation compared to H2O. Collectively, this study demonstrated that (i) different HuNoV strains within GII exhibited different sensitivities to high pressure, and (ii) HPP is capable of inactivating HuNoV GII strains by optimizing pressure parameters. IMPORTANCE: Human norovirus (HuNoV) is a leading cause of foodborne disease worldwide. Noroviruses are highly diverse, both antigenically and genetically. Genogroup II (GII) contains the majority of HuNoVs, with GII genotype 4 (GII.4) being the most prevalent. Recently, GII.1 and GII.6 have emerged and caused many outbreaks worldwide. However, the survival of these GII HuNoVs is poorly understood because they are uncultivable in vitro Using a novel receptor-binding assay conjugated with real-time RT-PCR, we found that GII HuNoVs had variable susceptibilities to high-pressure processing (HPP), which is one of the most promising food-processing technologies. The resistance of HuNoV strains to HPP ranked as follows: GII.1 > GII.6 > GII.4. This study highlights the ability of HPP to inactivate HuNoV and the need to optimize processing conditions based on HuNoV strain variability and sample matrix.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/genética
Manipulação de Alimentos
Genoma Viral
Norovirus/fisiologia
[Mh] Termos MeSH secundário: Animais
Mucinas Gástricas/química
Genótipo
Seres Humanos
Separação Imunomagnética
Norovirus/genética
Reação em Cadeia da Polimerase em Tempo Real
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Gastric Mucins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01575-16


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[PMID]:27454489
[Au] Autor:Chimuro T; Kuroyama H; Goso Y; Ishihara K; Kurihara M
[Ad] Endereço:Isehara Research Laboratory, Kanto Chemical Co., Inc., 21 Suzukawa, Isehara, Kanagawa 259-1146, Japan.
[Ti] Título:Discrimination of rat Brunner's gland carbohydrate antigens by site-specific monoclonal antibodies.
[So] Source:Carbohydr Res;432:76-82, 2016 Sep 02.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mucus produced and secreted by gastrointestinal mucosa contains various types of mucins equipped with unique sugar chains considered to play critical roles in protecting mucous membranes; therefore, the identification and verification of mucin sugar chains is important for understanding the underlying mechanisms. In our previous work, we generated three monoclonal antibodies (mAbs), RGM22, RGM26, and RGM42, which recognize sugar chains in rat gastric mucin. Here, we immunohistochemically analyzed the rat gastrointestinal mucosa and found that the antigens recognized by RGM22 and RGM42 were expressed in the rat antrum and Brunner's glands, whereas that recognized by RGM26 was detected in the antrum, but rarely in Brunner's glands. We found that these antibodies reacted with porcine gastric mucin-derived oligosaccharides bearing a common structure: GalNAcα1-3(Fucα1-2)Galß1-4GlcNAcß1-6GalNAc-ol. Moreover, epitope analysis revealed that RGM42 and RGM22 recognized α-linked GalNAc and GalNAcα1-3Gal, respectively, on the GalNAcα1-3(Fucα1-2)Gal structure, whereas RGM26 was specific for GalNAcα1-3(Fucα1-2)Gal. These results indicate that rat Brunner's glands express specific antigens bearing GalNAcα1-3Gal that are recognized by RGM22 and RGM42. Thus, RGM22, RGM26, and RGM42 with their unique antigen specificities could be useful tools for investigation of oligosaccharide diversity among mucins.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/metabolismo
Glândulas Duodenais/imunologia
Carboidratos/química
Mucinas Gástricas/análise
[Mh] Termos MeSH secundário: Animais
Sequência de Carboidratos
Carboidratos/análise
Carboidratos/imunologia
Epitopos/metabolismo
Mucinas Gástricas/química
Mucinas Gástricas/imunologia
Mucosa Intestinal/imunologia
Ratos
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Carbohydrates); 0 (Epitopes); 0 (Gastric Mucins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160726
[St] Status:MEDLINE



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